-- dump date   	20140618_212427
-- class       	Genbank::Contig
-- table       	contig_comment
-- id	comment
NC_005945.1	REVIEWED REFSEQ: This record has been curated by NCBI staff. TheREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225.REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference GenomeREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt GenomeREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmidREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the mainREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplishedREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  MostREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives ofREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still useREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation toREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull,REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull, PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries wereREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull, PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL),REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull, PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production GenomicsREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull, PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. to a coverage of 16x.REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull, PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. to a coverage of 16x. Finishing was performed at JGI-LANL starting with 39 contigs and 15REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull, PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. to a coverage of 16x. Finishing was performed at JGI-LANL starting with 39 contigs and 15 scaffolds. Repetitive regions were identified, assembled andREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull, PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. to a coverage of 16x. Finishing was performed at JGI-LANL starting with 39 contigs and 15 scaffolds. Repetitive regions were identified, assembled and finished individually using a local software package called RepFin.REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull, PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. to a coverage of 16x. Finishing was performed at JGI-LANL starting with 39 contigs and 15 scaffolds. Repetitive regions were identified, assembled and finished individually using a local software package called RepFin. Twenty four gaps were closed with primer walks and 15 by PCR. GeneREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull, PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. to a coverage of 16x. Finishing was performed at JGI-LANL starting with 39 contigs and 15 scaffolds. Repetitive regions were identified, assembled and finished individually using a local software package called RepFin. Twenty four gaps were closed with primer walks and 15 by PCR. Gene predictions were performed using Glimmer and tRNAs were identifiedREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull, PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. to a coverage of 16x. Finishing was performed at JGI-LANL starting with 39 contigs and 15 scaffolds. Repetitive regions were identified, assembled and finished individually using a local software package called RepFin. Twenty four gaps were closed with primer walks and 15 by PCR. Gene predictions were performed using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions wasREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull, PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. to a coverage of 16x. Finishing was performed at JGI-LANL starting with 39 contigs and 15 scaffolds. Repetitive regions were identified, assembled and finished individually using a local software package called RepFin. Twenty four gaps were closed with primer walks and 15 by PCR. Gene predictions were performed using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by searching the coding sequence against the PFam,REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull, PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. to a coverage of 16x. Finishing was performed at JGI-LANL starting with 39 contigs and 15 scaffolds. Repetitive regions were identified, assembled and finished individually using a local software package called RepFin. Twenty four gaps were closed with primer walks and 15 by PCR. Gene predictions were performed using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by searching the coding sequence against the PFam, BLOCKS, and Prodom databases. For the most part, gene definitionsREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull, PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. to a coverage of 16x. Finishing was performed at JGI-LANL starting with 39 contigs and 15 scaffolds. Repetitive regions were identified, assembled and finished individually using a local software package called RepFin. Twenty four gaps were closed with primer walks and 15 by PCR. Gene predictions were performed using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by searching the coding sequence against the PFam, BLOCKS, and Prodom databases. For the most part, gene definitions were transferred directly from the TIGR Bacillus anthracis AmesREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull, PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. to a coverage of 16x. Finishing was performed at JGI-LANL starting with 39 contigs and 15 scaffolds. Repetitive regions were identified, assembled and finished individually using a local software package called RepFin. Twenty four gaps were closed with primer walks and 15 by PCR. Gene predictions were performed using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by searching the coding sequence against the PFam, BLOCKS, and Prodom databases. For the most part, gene definitions were transferred directly from the TIGR Bacillus anthracis Ames GenBank records using a bi-directional best hit mapping between theREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull, PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. to a coverage of 16x. Finishing was performed at JGI-LANL starting with 39 contigs and 15 scaffolds. Repetitive regions were identified, assembled and finished individually using a local software package called RepFin. Twenty four gaps were closed with primer walks and 15 by PCR. Gene predictions were performed using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by searching the coding sequence against the PFam, BLOCKS, and Prodom databases. For the most part, gene definitions were transferred directly from the TIGR Bacillus anthracis Ames GenBank records using a bi-directional best hit mapping between the two genomes. A total of 5415 features have been annotated on theREVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull, PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. to a coverage of 16x. Finishing was performed at JGI-LANL starting with 39 contigs and 15 scaffolds. Repetitive regions were identified, assembled and finished individually using a local software package called RepFin. Twenty four gaps were closed with primer walks and 15 by PCR. Gene predictions were performed using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by searching the coding sequence against the PFam, BLOCKS, and Prodom databases. For the most part, gene definitions were transferred directly from the TIGR Bacillus anthracis Ames GenBank records using a bi-directional best hit mapping between the two genomes. A total of 5415 features have been annotated on the sequence record.REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AE017225. RefSeq Category: Reference Genome             UPR: UniProt Genome The Sterne strain of Bacillus anthracis is lacking the plasmid pXO2, resulting in an avirulent phenotype that maintains the main anthrax toxin. Prevention of anthrax in cattle can be accomplished by vaccination with living spores of the Sterne strain.  Most livestock vaccines in use throughout the world are derivatives of the live spore vaccine formulated by Sterne in 1937 and still use descendants of his strain 34F2 (Anthrax: the disease in relation to vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984 2:125-132 and Anthrax vaccines: past, present and future. Turnbull, PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. to a coverage of 16x. Finishing was performed at JGI-LANL starting with 39 contigs and 15 scaffolds. Repetitive regions were identified, assembled and finished individually using a local software package called RepFin. Twenty four gaps were closed with primer walks and 15 by PCR. Gene predictions were performed using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by searching the coding sequence against the PFam, BLOCKS, and Prodom databases. For the most part, gene definitions were transferred directly from the TIGR Bacillus anthracis Ames GenBank records using a bi-directional best hit mapping between the two genomes. A total of 5415 features have been annotated on the sequence record. COMPLETENESS: full length.