-- dump date   	20140618_222506
-- class       	Genbank::Contig
-- table       	contig_comment
-- id	comment
NC_009673.1	PROVISIONAL REFSEQ: This record has not yet been subject to finalPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.govPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei SorokinPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr)PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org)PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-StanfordPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGFPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps withPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. EachPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total errorPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data toPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of thisPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborativePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376)PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes:PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminantPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) byPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified asPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was furtherPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identifiedPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necroticPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to bePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studiesPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strainPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereusPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereus group (Sorokin et al, Appl Env Microbiol, 2006, 72, 1569). GenomicPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereus group (Sorokin et al, Appl Env Microbiol, 2006, 72, 1569). Genomic sequence clearly indicated that the strain is very different fromPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereus group (Sorokin et al, Appl Env Microbiol, 2006, 72, 1569). Genomic sequence clearly indicated that the strain is very different from other representatives of the group and should be assigned a newPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereus group (Sorokin et al, Appl Env Microbiol, 2006, 72, 1569). Genomic sequence clearly indicated that the strain is very different from other representatives of the group and should be assigned a new species status, for which we propose a name Bacillus cytotoxis.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereus group (Sorokin et al, Appl Env Microbiol, 2006, 72, 1569). Genomic sequence clearly indicated that the strain is very different from other representatives of the group and should be assigned a new species status, for which we propose a name Bacillus cytotoxis. However, assigning of the novel species status is hampered by thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereus group (Sorokin et al, Appl Env Microbiol, 2006, 72, 1569). Genomic sequence clearly indicated that the strain is very different from other representatives of the group and should be assigned a new species status, for which we propose a name Bacillus cytotoxis. However, assigning of the novel species status is hampered by the fact that only one such strain is described at the moment ofPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereus group (Sorokin et al, Appl Env Microbiol, 2006, 72, 1569). Genomic sequence clearly indicated that the strain is very different from other representatives of the group and should be assigned a new species status, for which we propose a name Bacillus cytotoxis. However, assigning of the novel species status is hampered by the fact that only one such strain is described at the moment of genomic sequencing being done (2005).PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000765. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereus group (Sorokin et al, Appl Env Microbiol, 2006, 72, 1569). Genomic sequence clearly indicated that the strain is very different from other representatives of the group and should be assigned a new species status, for which we propose a name Bacillus cytotoxis. However, assigning of the novel species status is hampered by the fact that only one such strain is described at the moment of genomic sequencing being done (2005). COMPLETENESS: full length.
NC_009674.1	PROVISIONAL REFSEQ: This record has not yet been subject to finalPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.govPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei SorokinPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr)PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org)PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-StanfordPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGFPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps withPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. EachPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total errorPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data toPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of thisPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborativePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376)PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes:PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminantPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) byPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified asPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was furtherPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identifiedPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necroticPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to bePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studiesPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strainPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereusPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereus group (Sorokin et al, Appl Env Microbiol, 2006, 72, 1569). GenomicPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereus group (Sorokin et al, Appl Env Microbiol, 2006, 72, 1569). Genomic sequence clearly indicated that the strain is very different fromPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereus group (Sorokin et al, Appl Env Microbiol, 2006, 72, 1569). Genomic sequence clearly indicated that the strain is very different from other representatives of the group and should be assigned a newPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereus group (Sorokin et al, Appl Env Microbiol, 2006, 72, 1569). Genomic sequence clearly indicated that the strain is very different from other representatives of the group and should be assigned a new species status, for which we propose a name Bacillus cytotoxis.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereus group (Sorokin et al, Appl Env Microbiol, 2006, 72, 1569). Genomic sequence clearly indicated that the strain is very different from other representatives of the group and should be assigned a new species status, for which we propose a name Bacillus cytotoxis. However, assigning of the novel species status is hampered by thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereus group (Sorokin et al, Appl Env Microbiol, 2006, 72, 1569). Genomic sequence clearly indicated that the strain is very different from other representatives of the group and should be assigned a new species status, for which we propose a name Bacillus cytotoxis. However, assigning of the novel species status is hampered by the fact that only one such strain is described at the moment ofPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereus group (Sorokin et al, Appl Env Microbiol, 2006, 72, 1569). Genomic sequence clearly indicated that the strain is very different from other representatives of the group and should be assigned a new species status, for which we propose a name Bacillus cytotoxis. However, assigning of the novel species status is hampered by the fact that only one such strain is described at the moment of genomic sequencing being done (2005).PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000764. URL -- http://www.jgi.doe.gov JGI Project ID: 4000557 Source DNA and bacteria available from Alexei Sorokin (alexei.sorokine@jouy.inra.fr) Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)           Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Finishing done by JGI-PGF Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of a least two independent clones. Each base pair has a minumum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376) Additional notes: Bacillus cereus NVH 398-91 was originally isolated as a contaminant of vegetable puree in a retirement house in Essone (France) by Marie-Laure De Buyser (AFSSA, France). The strain was identified as a cause of a severe food poisoning case. It was further characterized biochemically and a novel cytotoxin K was identified as a major cause of epithelial cell lysis leading to necrotic enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the strain was identified as Bacillus cereus, it appeared to be different from all known strains. Detailed phylogenetic studies using MLST approach placed the strain rather far from main strain clusters, but confirmed that the strain belongs to the B. cereus group (Sorokin et al, Appl Env Microbiol, 2006, 72, 1569). Genomic sequence clearly indicated that the strain is very different from other representatives of the group and should be assigned a new species status, for which we propose a name Bacillus cytotoxis. However, assigning of the novel species status is hampered by the fact that only one such strain is described at the moment of genomic sequencing being done (2005). COMPLETENESS: full length.