-- dump date   	20140618_225706
-- class       	Genbank::Contig
-- table       	contig_comment
-- id	comment
NC_008598.1	PROVISIONAL REFSEQ: This record has not yet been subject to finalPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspectedPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations SpecialPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracisPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L,PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, andPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid andPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in LosPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGIPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. DraftPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries providedPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed softwarePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and qualityPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled withPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). PossiblePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombingPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps betweenPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCRPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessaryPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achievesPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error ratePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error rate less than 1 in 100,000. Gene predictions were obtained usingPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error rate less than 1 in 100,000. Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysisPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error rate less than 1 in 100,000. Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequencesPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error rate less than 1 in 100,000. Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, BLOCKS and Prodom databases. Gene definitions andPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error rate less than 1 in 100,000. Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators atPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error rate less than 1 in 100,000. Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators at JGI-LANL, using BLAST results in addition to information from thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error rate less than 1 in 100,000. Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators at JGI-LANL, using BLAST results in addition to information from the basic analysis.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error rate less than 1 in 100,000. Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators at JGI-LANL, using BLAST results in addition to information from the basic analysis. COMPLETENESS: full length.
NC_008600.1	PROVISIONAL REFSEQ: This record has not yet been subject to finalPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspectedPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations SpecialPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracisPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L,PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, andPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid andPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in LosPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGIPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. DraftPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries providedPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed softwarePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and qualityPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled withPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). PossiblePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombingPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps betweenPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCRPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessaryPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achievesPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error ratePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error rate less than 1 in 100,000. Gene predictions were obtained usingPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error rate less than 1 in 100,000. Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysisPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error rate less than 1 in 100,000. Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequencesPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error rate less than 1 in 100,000. Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, BLOCKS and Prodom databases. Gene definitions andPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error rate less than 1 in 100,000. Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators atPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error rate less than 1 in 100,000. Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators at JGI-LANL, using BLAST results in addition to information from thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error rate less than 1 in 100,000. Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators at JGI-LANL, using BLAST results in addition to information from the basic analysis.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000485. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome.  The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment.  After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error rate less than 1 in 100,000. Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators at JGI-LANL, using BLAST results in addition to information from the basic analysis. COMPLETENESS: full length.