-- dump date   	20140619_022322
-- class       	Genbank::Contig
-- table       	contig_comment
-- id	comment
NC_015913.1	PROVISIONAL REFSEQ: This record has not yet been subject to finalPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents ofPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determinedPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) andPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequencePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 largePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled usingPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) withPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) with a total of 25,257 Sanger sequencing reads from plasmid libraries inPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) with a total of 25,257 Sanger sequencing reads from plasmid libraries in the 3 kb and 10 kb size ranges. Gap closing and re-sequencing ofPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) with a total of 25,257 Sanger sequencing reads from plasmid libraries in the 3 kb and 10 kb size ranges. Gap closing and re-sequencing of low quality regions were performed by sequencing of PCR productsPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) with a total of 25,257 Sanger sequencing reads from plasmid libraries in the 3 kb and 10 kb size ranges. Gap closing and re-sequencing of low quality regions were performed by sequencing of PCR products and appropriate plasmid clones. Automated annotation of the genomePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) with a total of 25,257 Sanger sequencing reads from plasmid libraries in the 3 kb and 10 kb size ranges. Gap closing and re-sequencing of low quality regions were performed by sequencing of PCR products and appropriate plasmid clones. Automated annotation of the genome sequence was carried out with the Microbial Genome AnnotationPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) with a total of 25,257 Sanger sequencing reads from plasmid libraries in the 3 kb and 10 kb size ranges. Gap closing and re-sequencing of low quality regions were performed by sequencing of PCR products and appropriate plasmid clones. Automated annotation of the genome sequence was carried out with the Microbial Genome Annotation Pipeline (MiGAP)PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) with a total of 25,257 Sanger sequencing reads from plasmid libraries in the 3 kb and 10 kb size ranges. Gap closing and re-sequencing of low quality regions were performed by sequencing of PCR products and appropriate plasmid clones. Automated annotation of the genome sequence was carried out with the Microbial Genome Annotation Pipeline (MiGAP) (https://migap.lifesciencedb.jp/mgap/jsp/index.jsp), and manualPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) with a total of 25,257 Sanger sequencing reads from plasmid libraries in the 3 kb and 10 kb size ranges. Gap closing and re-sequencing of low quality regions were performed by sequencing of PCR products and appropriate plasmid clones. Automated annotation of the genome sequence was carried out with the Microbial Genome Annotation Pipeline (MiGAP) (https://migap.lifesciencedb.jp/mgap/jsp/index.jsp), and manual curation was done using the in silico Molecular Cloning GenomicsPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) with a total of 25,257 Sanger sequencing reads from plasmid libraries in the 3 kb and 10 kb size ranges. Gap closing and re-sequencing of low quality regions were performed by sequencing of PCR products and appropriate plasmid clones. Automated annotation of the genome sequence was carried out with the Microbial Genome Annotation Pipeline (MiGAP) (https://migap.lifesciencedb.jp/mgap/jsp/index.jsp), and manual curation was done using the in silico Molecular Cloning Genomics Edition (IMC-GE) software (Insilico biology, Yokohama, Japan).PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) with a total of 25,257 Sanger sequencing reads from plasmid libraries in the 3 kb and 10 kb size ranges. Gap closing and re-sequencing of low quality regions were performed by sequencing of PCR products and appropriate plasmid clones. Automated annotation of the genome sequence was carried out with the Microbial Genome Annotation Pipeline (MiGAP) (https://migap.lifesciencedb.jp/mgap/jsp/index.jsp), and manual curation was done using the in silico Molecular Cloning Genomics Edition (IMC-GE) software (Insilico biology, Yokohama, Japan). sequencing center: Center for Omics and Bioinfomatics, UniversityPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) with a total of 25,257 Sanger sequencing reads from plasmid libraries in the 3 kb and 10 kb size ranges. Gap closing and re-sequencing of low quality regions were performed by sequencing of PCR products and appropriate plasmid clones. Automated annotation of the genome sequence was carried out with the Microbial Genome Annotation Pipeline (MiGAP) (https://migap.lifesciencedb.jp/mgap/jsp/index.jsp), and manual curation was done using the in silico Molecular Cloning Genomics Edition (IMC-GE) software (Insilico biology, Yokohama, Japan). sequencing center: Center for Omics and Bioinfomatics, University of Tokyo.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) with a total of 25,257 Sanger sequencing reads from plasmid libraries in the 3 kb and 10 kb size ranges. Gap closing and re-sequencing of low quality regions were performed by sequencing of PCR products and appropriate plasmid clones. Automated annotation of the genome sequence was carried out with the Microbial Genome Annotation Pipeline (MiGAP) (https://migap.lifesciencedb.jp/mgap/jsp/index.jsp), and manual curation was done using the in silico Molecular Cloning Genomics Edition (IMC-GE) software (Insilico biology, Yokohama, Japan). sequencing center: Center for Omics and Bioinfomatics, University of Tokyo. ##Genome-Assembly-Data-START##PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) with a total of 25,257 Sanger sequencing reads from plasmid libraries in the 3 kb and 10 kb size ranges. Gap closing and re-sequencing of low quality regions were performed by sequencing of PCR products and appropriate plasmid clones. Automated annotation of the genome sequence was carried out with the Microbial Genome Annotation Pipeline (MiGAP) (https://migap.lifesciencedb.jp/mgap/jsp/index.jsp), and manual curation was done using the in silico Molecular Cloning Genomics Edition (IMC-GE) software (Insilico biology, Yokohama, Japan). sequencing center: Center for Omics and Bioinfomatics, University of Tokyo. ##Genome-Assembly-Data-START## Assembly Method       :: Newbler; PhrapPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) with a total of 25,257 Sanger sequencing reads from plasmid libraries in the 3 kb and 10 kb size ranges. Gap closing and re-sequencing of low quality regions were performed by sequencing of PCR products and appropriate plasmid clones. Automated annotation of the genome sequence was carried out with the Microbial Genome Annotation Pipeline (MiGAP) (https://migap.lifesciencedb.jp/mgap/jsp/index.jsp), and manual curation was done using the in silico Molecular Cloning Genomics Edition (IMC-GE) software (Insilico biology, Yokohama, Japan). sequencing center: Center for Omics and Bioinfomatics, University of Tokyo. ##Genome-Assembly-Data-START## Assembly Method       :: Newbler; Phrap Genome Coverage       :: 30xPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) with a total of 25,257 Sanger sequencing reads from plasmid libraries in the 3 kb and 10 kb size ranges. Gap closing and re-sequencing of low quality regions were performed by sequencing of PCR products and appropriate plasmid clones. Automated annotation of the genome sequence was carried out with the Microbial Genome Annotation Pipeline (MiGAP) (https://migap.lifesciencedb.jp/mgap/jsp/index.jsp), and manual curation was done using the in silico Molecular Cloning Genomics Edition (IMC-GE) software (Insilico biology, Yokohama, Japan). sequencing center: Center for Omics and Bioinfomatics, University of Tokyo. ##Genome-Assembly-Data-START## Assembly Method       :: Newbler; Phrap Genome Coverage       :: 30x Sequencing Technology :: 454 GS FLX; SangerPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) with a total of 25,257 Sanger sequencing reads from plasmid libraries in the 3 kb and 10 kb size ranges. Gap closing and re-sequencing of low quality regions were performed by sequencing of PCR products and appropriate plasmid clones. Automated annotation of the genome sequence was carried out with the Microbial Genome Annotation Pipeline (MiGAP) (https://migap.lifesciencedb.jp/mgap/jsp/index.jsp), and manual curation was done using the in silico Molecular Cloning Genomics Edition (IMC-GE) software (Insilico biology, Yokohama, Japan). sequencing center: Center for Omics and Bioinfomatics, University of Tokyo. ##Genome-Assembly-Data-START## Assembly Method       :: Newbler; Phrap Genome Coverage       :: 30x Sequencing Technology :: 454 GS FLX; Sanger ##Genome-Assembly-Data-END##PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AP012202. The genomic DNA of SFB was extracted from the caecal contents of SFB-gnotobiotic mice. The genome sequence of SFB was determined using a combined strategy of 454 pyrosequencing (GS FLX; Roche) and Sanger sequencing. A total 234,915 reads counting up to 34,357,230 bases, were obtained from a GS FLX sequencing run. The sequence reads were assembled with the GS Assembler software into 242 large contigs (_ 500 bp). The GS FLX contigs were then reassembled using the Phred/Phrap/Consed software package (http://www.phrap.com) with a total of 25,257 Sanger sequencing reads from plasmid libraries in the 3 kb and 10 kb size ranges. Gap closing and re-sequencing of low quality regions were performed by sequencing of PCR products and appropriate plasmid clones. Automated annotation of the genome sequence was carried out with the Microbial Genome Annotation Pipeline (MiGAP) (https://migap.lifesciencedb.jp/mgap/jsp/index.jsp), and manual curation was done using the in silico Molecular Cloning Genomics Edition (IMC-GE) software (Insilico biology, Yokohama, Japan). sequencing center: Center for Omics and Bioinfomatics, University of Tokyo. ##Genome-Assembly-Data-START## Assembly Method       :: Newbler; Phrap Genome Coverage       :: 30x Sequencing Technology :: 454 GS FLX; Sanger ##Genome-Assembly-Data-END## COMPLETENESS: full length.