-- dump date 20140619_080843 -- class Genbank::Feature -- table feature -- table main -- field 1 id -- field 2 type -- field 3 name -- field 4 contig -- field 5 start_pos -- field 6 end_pos -- field 7 strand -- field 8 description -- field 9 chrom_position -- field 10 organism -- field 11 GeneID -- header -- id type name contig start_pos end_pos strand description chrom_position organism GeneID 866768000001 SEQ_END SEQ_END NC_012947.1 4570938 4570938 DR NC_012947.1; contig end 4570938..4570938 Escherichia coli 'BL21-Gold(DE3)pLysS AG' YP_003034267.1 CDS dnaA NC_012947.1 347 1750 D binds to the dnaA-box as an ATP-bound complex at the origin of replication during the initiation of chromosomal replication; can also affect transcription of multiple genes including itself.; chromosomal replication initiation protein 347..1750 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156697 YP_003034268.1 CDS ECBD_0002 NC_012947.1 1755 2855 D binds the polymerase to DNA and acts as a sliding clamp; DNA polymerase III subunit beta 1755..2855 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157826 YP_003034269.1 CDS recF NC_012947.1 2855 3928 D Required for DNA replication; binds preferentially to single-stranded, linear DNA; recombination protein F 2855..3928 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157827 YP_003034270.1 CDS gyrB NC_012947.1 3957 6371 D negatively supercoils closed circular double-stranded DNA; DNA gyrase subunit B 3957..6371 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157828 YP_003034271.1 CDS ECBD_0005 NC_012947.1 6611 7009 D PFAM: protein of unknown function DUF937; KEGG: ssn:SSON_3648 hypothetical protein; hypothetical protein 6611..7009 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157019 YP_003034272.1 CDS ECBD_0006 NC_012947.1 7124 7936 D YidA; catalyzes the dephosphorylation of erythrose 4-phosphate (preferred substrate), mannose 1-phosphate and p-nitrophenyl phosphate; hydrolyzes the alpha-D-glucose-1-phosphate but not the beta form; member of the haloacid dehalogenase-like hydrolases superfamily and Cof family of proteins; sugar phosphatase 7124..7936 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160210 YP_003034273.1 CDS ECBD_0007 NC_012947.1 7982 8638 R KEGG: sfl:SF3768 hypothetical protein; hypothetical protein complement(7982..8638) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160211 YP_003034274.1 CDS ECBD_0008 NC_012947.1 8916 9605 D PFAM: GntR family transcriptional regulator; regulatory protein GntR HTH; SMART: regulatory protein GntR HTH; KEGG: sfx:S4002 regulator protein for dgo operon; GntR family transcriptional regulator 8916..9605 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160212 YP_003034275.1 CDS ECBD_0009 NC_012947.1 9602 10480 D PFAM: 2-keto-3-deoxy-galactonokinase; KEGG: ssn:SSON_3644 2-oxo-3-deoxygalactonate kinase; 2-dehydro-3-deoxygalactonokinase 9602..10480 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160213 YP_003034276.1 CDS ECBD_0010 NC_012947.1 10464 11081 D catalyzes the formation of D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-galactonate 6-phosphate; functions in galactonate metabolism; 2-dehydro-3-deoxy-6-phosphogalactonate aldolase 10464..11081 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160214 YP_003034277.1 CDS ECBD_0011 NC_012947.1 11078 12226 D PFAM: mandelate racemase/muconate lactonizing protein; galactonate dehydratase 11078..12226 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160215 YP_003034278.1 CDS ECBD_0012 NC_012947.1 12346 13638 D TIGRFAM: d-galactonate transporter; phosphoglycerate transporter; PFAM: major facilitator superfamily MFS_1; general substrate transporter; KEGG: ssn:SSON_3642 D-galactonate transport; d-galactonate transporter 12346..13638 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160216 YP_003034279.1 CDS ECBD_0013 NC_012947.1 13635 14699 R FAD/NAD(P)-binding domain; oxidoreductase complement(13635..14699) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160217 YP_003034280.1 CDS ECBD_0014 NC_012947.1 14800 16014 D KEGG: ssn:SSON_3640 hypothetical protein; hypothetical protein 14800..16014 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160218 YP_003034281.1 CDS ECBD_0015 NC_012947.1 16016 16423 R PFAM: protein of unknown function DUF1375; KEGG: sfv:SFV_3823 hypothetical protein; hypothetical protein complement(16016..16423) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160219 YP_003034282.1 CDS ECBD_0016 NC_012947.1 16654 17067 D with IbpB associates with aggregated proteins to stabilize and protect them from irreversible denaturation and extensive proteolysis during heat shock and oxidative stress; heat shock protein IbpA 16654..17067 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160220 YP_003034283.1 CDS ECBD_0017 NC_012947.1 17179 17607 D 16 kDa heat shock protein B; associates with aggregated proteins, together with ibpA, to stabilize and protect them from irreversible denaturation and extensive proteolysis during heat shock and oxidative stress; ATP-independent; heat shock chaperone IbpB 17179..17607 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160221 YP_003034284.1 CDS ECBD_0018 NC_012947.1 17803 19464 D TIGRFAM: YidE/YbjL duplication; PFAM: YidE/YbjL duplication domain protein; TrkA-C domain protein; KEGG: sfx:S3992 hypothetical protein; hypothetical protein 17803..19464 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160222 YP_003034285.1 CDS ECBD_0020 NC_012947.1 20394 22010 D TIGRFAM: PTS system, alpha-glucoside-specific IIBC subunit; PTS system, maltose and glucose-specific subfamily, IIC subunit; PTS system, glucose-like IIB subunint; PFAM: phosphotransferase system EIIC; phosphotransferase system PTS EIIB protein; KEGG: ssn:SSON_3634 phosphotransferase system enzyme IIBC component; PTS system, alpha-glucoside-specific IIBC subunit 20394..22010 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160224 YP_003034286.1 CDS ECBD_0021 NC_012947.1 22010 23332 D PFAM: glycoside hydrolase family 4; KEGG: sdy:SDY_4168 6-phospho-beta-glucosidase; glycoside hydrolase family 4 22010..23332 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160225 YP_003034287.1 CDS ECBD_0022 NC_012947.1 23329 24222 R PFAM: helix-turn-helix- domain containing protein AraC type; SMART: helix-turn-helix- domain containing protein AraC type; AraC family transcriptional regulator complement(23329..24222) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160226 YP_003034288.1 CDS ECBD_0023 NC_012947.1 24389 26104 D uncharacterized member of the SSS superfamily of sodium-dependent solute transporters; unknown function; symporter YidK 24389..26104 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160227 YP_003034289.1 CDS ECBD_0024 NC_012947.1 26101 27594 D PFAM: sulfatase; sulfatase 26101..27594 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160228 YP_003034290.1 CDS ECBD_0025 NC_012947.1 27641 28090 R KEGG: ssn:SSON_3631 hypothetical protein; hypothetical protein complement(27641..28090) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160229 YP_003034291.1 CDS ECBD_0026 NC_012947.1 28199 28546 D PFAM: protein of unknown function DUF202; KEGG: sdy:SDY_4160 hypothetical protein; hypothetical protein 28199..28546 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160230 YP_003034292.1 CDS ECBD_0027 NC_012947.1 28536 28898 D KEGG: sfv:SFV_3835 hypothetical protein; hypothetical protein 28536..28898 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160231 YP_003034293.1 CDS ECBD_0028 NC_012947.1 28895 29392 D KEGG: sfv:SFV_3836 transcriptional regulator; transcriptional regulator 28895..29392 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160232 YP_003034294.1 CDS emrD NC_012947.1 29400 30584 R multidrug efflux protein involved in adaptation to low energy shock; multidrug resistance protein D complement(29400..30584) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160233 YP_003034295.1 CDS ECBD_0030 NC_012947.1 30741 30989 D KEGG: sbc:SbBS512_E4246 hypothetical protein; hypothetical protein 30741..30989 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160234 YP_003034296.1 CDS ECBD_0031 NC_012947.1 31241 31723 R KEGG: ses:SARI_03851 hypothetical protein; hypothetical protein complement(31241..31723) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160235 YP_003034297.1 CDS ECBD_0032 NC_012947.1 31722 33410 D TIGRFAM: acetolactate synthase, large subunit, biosynthetic type; PFAM: thiamine pyrophosphate protein TPP binding domain protein; thiamine pyrophosphate protein central region; thiamine pyrophosphate protein domain protein TPP-binding; KEGG: elf:LF82_1102 acetolactate synthase isozyme I large subunit; acetolactate synthase catalytic subunit 31722..33410 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160236 YP_003034298.1 CDS ECBD_0033 NC_012947.1 33414 33704 D with IlvB catalyzes the formation of 2-acetolactate from pyruvate, the small subunit is required for full activity and valine sensitivity; E.coli produces 3 isoenzymes of acetolactate synthase which differ in specificity to substrates, valine sensitivity and affinity for cofactors; also known as acetolactate synthase small subunit; acetolactate synthase 1 regulatory subunit 33414..33704 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160237 YP_003034299.1 CDS ECBD_0034 NC_012947.1 33779 34369 D response regulator in two-component regulatory system wtih UhpB; phosphorylated UhpA is a positive activator uhpT, a hexose phosphates transporter; DNA-binding transcriptional activator UhpA 33779..34369 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160238 YP_003034300.1 CDS ECBD_0035 NC_012947.1 34369 35871 D Member of the two-component regulatory system UhpB/UhpA involved in the regulation of the uptake of hexose ph; sensory histidine kinase UhpB 34369..35871 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160239 YP_003034301.1 CDS ECBD_0036 NC_012947.1 35881 37200 D membrane protein regulates uhpT expression; regulatory protein UhpC 35881..37200 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160240 YP_003034302.1 CDS uhpT NC_012947.1 37338 38729 D cytoplasmic membrane protein that functions as a monomer; catalyzes the active transport of sugar-phosphates such as glucose-6-phosphate with the obligatory exchange of inorganic phosphate or organophosphate; sugar phosphate antiporter 37338..38729 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160241 YP_003034303.1 CDS ECBD_0038 NC_012947.1 38775 40541 R catalyzes the formation of hypoxanthine from adenine; in E. coli this activity has been detected in mutant strains but not in wild type; cryptic adenine deaminase complement(38775..40541) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160242 YP_003034304.1 CDS ECBD_0039 NC_012947.1 40716 42050 D PFAM: xanthine/uracil/vitamin C permease; sulfate transporter; KEGG: sbc:SbBS512_E4255 inorganic anion transporter sulfate permease (SulP) family; xanthine/uracil/vitamin C permease 40716..42050 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160243 YP_003034305.1 CDS ECBD_0040 NC_012947.1 42103 42555 D PFAM: protein of unknown function DUF1198; KEGG: sdy:SDY_4144 hypothetical protein; hypothetical protein 42103..42555 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160244 YP_003034306.1 CDS nepI NC_012947.1 42766 43956 D experimental results in Escherichia coli suggest that this gene encodes an inosine export protein; member of major facilitator superfamily; MFS; ribonucleoside transporter 42766..43956 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160245 YP_003034307.1 CDS ECBD_0042 NC_012947.1 43997 44290 R KEGG: sbo:SBO_3710 transporter; transporter complement(43997..44290) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160246 YP_003034308.1 CDS ECBD_0043 NC_012947.1 44295 44414 R hypothetical protein complement(44295..44414) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160247 YP_003034309.1 CDS ECBD_0044 NC_012947.1 44512 45330 D TIGRFAM: lipoprotein, YaeC family; PFAM: NLPA lipoprotein; KEGG: sdy:SDY_4499 cytoplasmic membrane lipoprotein-28; cytoplasmic membrane lipoprotein-28 44512..45330 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160248 YP_003034310.1 CDS ECBD_0045 NC_012947.1 45334 46257 R TIGRFAM: carboxylate/amino acid/amine transporter; PFAM: protein of unknown function DUF6 transmembrane; KEGG: elf:LF82_3359 uncharacterized inner membrane transporter YicL; carboxylate/amino acid/amine transporter complement(45334..46257) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160249 YP_003034311.1 CDS ECBD_0046 NC_012947.1 46368 47552 R TIGRFAM: sugar efflux transporter; PFAM: major facilitator superfamily MFS_1; sugar efflux transporter complement(46368..47552) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160250 YP_003034312.1 CDS ECBD_0047 NC_012947.1 47934 48080 R KEGG: ypb:YPTS_4098 hypothetical protein; hypothetical protein complement(47934..48080) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160251 YP_003034313.1 CDS ECBD_0048 NC_012947.1 48342 49187 R KEGG: seh:SeHA_C4673 hypothetical protein; hypothetical protein complement(48342..49187) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160252 YP_003034314.1 CDS ECBD_0049 NC_012947.1 49272 49469 R PFAM: protein of unknown function DUF957; KEGG: sfl:SF3001 hypothetical protein; hypothetical protein complement(49272..49469) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160253 YP_003034315.1 CDS ECBD_0050 NC_012947.1 49481 49969 R KEGG: sfx:S3205 hypothetical protein; hypothetical protein complement(49481..49969) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160254 YP_003034316.1 CDS ECBD_0051 NC_012947.1 49966 50331 R PFAM: protein of unknown function DUF1219; KEGG: sfx:S3204 hypothetical protein; hypothetical protein complement(49966..50331) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160255 YP_003034317.1 CDS ECBD_0052 NC_012947.1 50229 51353 R PFAM: Integrase catalytic region; Integrase catalytic region complement(50229..51353) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160256 YP_003034318.1 CDS ECBD_0053 NC_012947.1 51310 51666 R PFAM: transposase IS3/IS911 family protein; KEGG: sfv:SFV_4359 ISEhe3 orfA; transposase IS3/IS911 family protein complement(51310..51666) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160257 YP_003034319.1 CDS ECBD_0054 NC_012947.1 52699 56196 R TIGRFAM: outer membrane autotransporter barrel domain protein; autotransporter-associated beta strand repeat protein; PFAM: Autotransporter beta- domain protein; Pertactin; KEGG: cko:CKO_00426 hypothetical protein; outer membrane autotransporter barrel domain protein complement(52699..56196) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160258 YP_003034320.1 CDS ECBD_0056 NC_012947.1 57475 57630 D hypothetical protein 57475..57630 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160260 YP_003034321.1 CDS ECBD_0057 NC_012947.1 57679 58053 D KEGG: kpn:KPN_pKPN5p08236 hypothetical protein; hypothetical protein 57679..58053 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160261 YP_003034322.1 CDS ECBD_0058 NC_012947.1 58206 58517 D PFAM: transposase IS3/IS911 family protein; transposase IS3/IS911 family protein 58206..58517 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160262 YP_003034323.1 CDS ECBD_0059 NC_012947.1 58364 59527 R KEGG: seh:SeHA_A0075 TrbC; TrbC complement(58364..59527) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160263 YP_003034324.1 CDS ECBD_0060 NC_012947.1 59581 60066 R TIGRFAM: single-strand binding protein; PFAM: single-strand binding protein/Primosomal replication protein n; KEGG: cko:CKO_03842 single-stranded DNA-binding protein; single-strand binding protein complement(59581..60066) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160264 YP_003034325.1 CDS ECBD_0061 NC_012947.1 60714 63095 R PFAM: glycoside hydrolase family 31; KEGG: ssn:SSON_0800 glucosidase; glucan 1,3-alpha-glucosidase complement(60714..63095) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160265 YP_003034326.1 CDS ECBD_0062 NC_012947.1 63110 64411 R PFAM: major facilitator superfamily MFS_1; KEGG: ssn:SSON_0801 hexuronate transporter; major facilitator superfamily MFS_1 complement(63110..64411) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160266 YP_003034327.1 CDS ECBD_0063 NC_012947.1 64673 65743 D PFAM: periplasmic binding protein/LacI transcriptional regulator; regulatory protein LacI; SMART: regulatory protein LacI; KEGG: ssn:SSON_0802 transcriptional regulator; LacI family transcriptional regulator 64673..65743 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160267 YP_003034328.1 CDS ECBD_0064 NC_012947.1 65768 65998 R hypothetical protein complement(65768..65998) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160268 YP_003034329.1 CDS ECBD_0065 NC_012947.1 66141 67184 R KEGG: sfv:SFV_3869 hypothetical protein; hypothetical protein complement(66141..67184) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160269 YP_003034330.1 CDS ECBD_0066 NC_012947.1 67595 68779 R PFAM: integrase; KEGG: ssn:SSON_3744 integrase; integrase complement(67595..68779) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160270 YP_003034331.1 CDS ECBD_0067 NC_012947.1 69466 70848 D may be involved in the transport of galactosides-pentoses-hexuronides; transporter 69466..70848 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160272 YP_003034332.1 CDS ECBD_0068 NC_012947.1 70858 73176 D catalyzes the transfer of alpha-xylosyl residue from alpha-xyloside to xylose, glucose, mannose, fructose, maltose, isomaltose, nigerose, kojibiose, sucrose, and trehalose; shows higher activity against alpha-xylosyl fluoride, isoprimeverose (6-O-alpha-xylopyranosyl-glucopyranose), and alpha-xyloside in xyloglucan oligosaccharides; alpha-xylosidase YicI 70858..73176 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160273 YP_003034333.1 CDS ECBD_0069 NC_012947.1 73229 74938 R PFAM: AsmA family protein; KEGG: sfx:S4074 hypothetical protein; AsmA family protein complement(73229..74938) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160274 YP_003034334.1 CDS ECBD_0070 NC_012947.1 75059 76450 R TIGRFAM: uracil-xanthine permease; PFAM: xanthine/uracil/vitamin C permease; KEGG: ssn:SSON_3751 transport protein; uracil-xanthine permease complement(75059..76450) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160275 YP_003034335.1 CDS ECBD_0071 NC_012947.1 76730 77935 D TIGRFAM: sodium/glutamate symporter; PFAM: sodium/glutamate symporter; KEGG: sdy:SDY_4085 glutamate transport; sodium/glutamate symporter 76730..77935 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160276 YP_003034336.1 CDS ECBD_0072 NC_012947.1 77938 78804 D KEGG: efe:EFER_3945 hypothetical protein; hypothetical protein 77938..78804 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160277 YP_003034337.1 CDS ECBD_0073 NC_012947.1 78789 80870 R catalyzes branch migration in Holliday junction intermediates; ATP-dependent DNA helicase RecG complement(78789..80870) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160278 YP_003034338.1 CDS ECBD_0074 NC_012947.1 80876 81565 R specifically modifies tRNA at position G18; tRNA guanosine-2'-O-methyltransferase complement(80876..81565) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160279 YP_003034339.1 CDS ECBD_0075 NC_012947.1 81572 83680 R KEGG: sfv:SFV_3879 bifunctional (p)ppGpp synthetase II/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase; TIGRFAM: RelA/SpoT family protein; PFAM: RelA/SpoT domain protein; TGS domain protein; metal-dependent phosphohydrolase HD sub domain; SMART: metal-dependent phosphohydrolase HD region; bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase complement(81572..83680) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160280 YP_003034340.1 CDS rpoZ NC_012947.1 83699 83974 R promotes RNA polymerase assembly or stability; latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and subunit alphas; DNA-directed RNA polymerase subunit omega complement(83699..83974) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160281 YP_003034341.1 CDS gmk NC_012947.1 84029 84652 R Essential for recycling GMP and indirectly, cGMP; guanylate kinase complement(84029..84652) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160282 YP_003034342.1 CDS ligB NC_012947.1 84910 86592 D this ligase is similar to LigA but it lacks the C-terminal BRCT domain; catalyzes strand joining of nicked DNA in the presence of a divalent cation and NAD+; NAD-dependent DNA ligase LigB 84910..86592 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160283 YP_003034343.1 CDS ECBD_0079 NC_012947.1 86589 87206 R PFAM: protein of unknown function UPF0126; KEGG: sdy:SDY_4077 hypothetical protein; hypothetical protein complement(86589..87206) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160284 YP_003034344.1 CDS dinD NC_012947.1 87497 88321 R KEGG: sbc:SbBS512_E4096 DNA-damage-inducible protein D; DNA-damage-inducible protein D complement(87497..88321) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160285 YP_003034345.1 CDS ECBD_0081 NC_012947.1 88385 88525 D KEGG: sbc:SbBS512_E4095 hypothetical protein; hypothetical protein 88385..88525 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160286 YP_003034346.1 CDS ECBD_0082 NC_012947.1 88542 89405 R PFAM: YicC domain protein; domain of unknown function DUF1732; KEGG: ssn:SSON_3762 hypothetical protein; hypothetical protein complement(88542..89405) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160287 YP_003034347.1 CDS rph NC_012947.1 89532 90248 D RNase PH; tRNA nucleotidyltransferase; forms hexamers in Bacillus subtilis; phosphoroltic 3'-5' exoribonuclease; involved in maturation of tRNA precursors and removes terminal nucleotides near CCA acceptor arms of mature tRNAs; ribonuclease PH 89532..90248 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160288 YP_003034348.1 CDS pyrE NC_012947.1 90314 90955 D involved in fifth step of pyrimidine biosynthesis; converts orotidine 5'-phosphate and diphosphate to orotate and 5-phospho-alpha-D-ribose 1-diphosphate; orotate phosphoribosyltransferase 90314..90955 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160289 YP_003034349.1 CDS slmA NC_012947.1 90992 91588 R FtsZ binding protein; synthetically lethal with a defect in the Min system; this protein is the first identified nucleoid occlusion factor which works along with the Min system to properly position the FtsZ ring assembly; nucleoid occlusion protein complement(90992..91588) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160290 YP_003034350.1 CDS dut NC_012947.1 91695 92153 R catalyzes the formation of dUMP from dUTP; deoxyuridine 5'-triphosphate nucleotidohydrolase complement(91695..92153) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160373 YP_003034351.1 CDS ECBD_0087 NC_012947.1 92131 93351 R catalyzes the conjugation of cysteine to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, which is then decarboxylated to form 4'-phosphopantotheine; bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase complement(92131..93351) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156337 YP_003034352.1 CDS radC NC_012947.1 93523 94191 D Involved in DNA double-strand break repair and recombination. Promotes the annealing of complementary single-stranded DNA and by simulation of RAD51 recombinase; DNA repair protein RadC 93523..94191 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156338 YP_003034353.1 CDS rpmB NC_012947.1 94408 94644 D required for 70S ribosome assembly; 50S ribosomal protein L28 94408..94644 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156339 YP_003034354.1 CDS rpmG NC_012947.1 94665 94832 D in Escherichia coli BM108, a mutation that results in lack of L33 synthesis had no effect on ribosome synthesis or function; there are paralogous genes in several bacterial genomes, and a CXXC motif for zinc binding and an upstream regulation region of the paralog lacking this motif that are regulated by zinc similar to other ribosomal proteins like L31; the proteins in this group lack the CXXC motif; 50S ribosomal protein L33 94665..94832 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156340 YP_003034355.1 CDS ECBD_0091 NC_012947.1 94930 95739 D Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases; formamidopyrimidine-DNA glycosylase 94930..95739 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156341 YP_003034356.1 CDS coaD NC_012947.1 95778 96257 R Catalyzes the conversion of ATP and pantetheine 4'-phosphate to diphosphate and 3'-dephospho-coA; phosphopantetheine adenylyltransferase complement(95778..96257) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156342 YP_003034357.1 CDS ECBD_0093 NC_012947.1 96265 97542 R catalyzes the transfer of 2-keto-3-deoxy-D-manno-octulosonic acid to lipid A; 3-deoxy-D-manno-octulosonic-acid transferase complement(96265..97542) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156343 YP_003034358.1 CDS ECBD_0094 NC_012947.1 97991 99013 D TIGRFAM: lipopolysaccharide heptosyltransferase III; PFAM: glycosyl transferase family 9; KEGG: sdy:SDY_4062 lipopolysaccharide core biosynthesis protein; lipopolysaccharide core biosynthesis protein 97991..99013 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156344 YP_003034359.1 CDS ECBD_0095 NC_012947.1 99010 100134 D PFAM: glycosyl transferase group 1; KEGG: sfv:SFV_3900 LPS alpha1,3-glucosyltransferase; glycosyl transferase group 1 99010..100134 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156345 YP_003034360.1 CDS ECBD_0096 NC_012947.1 100127 100924 D PFAM: lipopolysaccharide kinase; KEGG: sdy:SDY_4060 LPS biosynthesis enzyme; lipopolysaccharide kinase 100127..100924 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156346 YP_003034361.1 CDS ECBD_0097 NC_012947.1 100940 101956 D PFAM: glycosyl transferase family 8; glycosyl transferase family 8 domain protein; KEGG: sbo:SBO_3631 UDP-D-galactose:(glucosyl)lipopolysaccharide-alpha-1, 3-D-galactosylt ransferase; Lipopolysaccharide 3-alpha-galactosyltransferase 100940..101956 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156347 YP_003034362.1 CDS ECBD_0099 NC_012947.1 102527 102802 D PFAM: Insertion element protein; KEGG: dps:DP0861 insertion element iso-IS1d protein (InsA); Insertion element protein 102527..102802 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156349 YP_003034363.1 CDS ECBD_0100 NC_012947.1 102721 103224 D PFAM: IS1 transposase; KEGG: dps:DP0860 insertion element IS1 1/5/6 protein (InsB); IS1 transposase 102721..103224 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156350 YP_003034364.1 CDS ECBD_0101 NC_012947.1 103754 104446 D PFAM: lipopolysaccharide core biosynthesis protein; KEGG: sdy:SDY_4057 lipopolysaccharide core biosynthesis protein; lipopolysaccharide core biosynthesis protein 103754..104446 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156351 YP_003034365.1 CDS ECBD_0102 NC_012947.1 104472 105497 D PFAM: glycosyl transferase family 8; glycosyl transferase family 8 domain protein; KEGG: ssn:SSON_3781 UDP-galactose:(galactosyl) LPS alpha1,2-galactosyltransferase; Lipopolysaccharide glucosyltransferase I 104472..105497 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156352 YP_003034366.1 CDS ECBD_0103 NC_012947.1 105582 106565 D PFAM: glycosyl transferase family 2; KEGG: ssn:SSON_3782 beta1,3-glucosyltransferase; glycosyl transferase family 2 105582..106565 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156353 YP_003034367.1 CDS ECBD_0104 NC_012947.1 106611 107864 D PFAM: O-antigen polymerase; KEGG: ssn:SSON_3783 lipid A-core, surface polymer ligase; O-antigen polymerase 106611..107864 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156354 YP_003034368.1 CDS ECBD_0105 NC_012947.1 107934 108914 R TIGRFAM: lipopolysaccharide heptosyltransferase I; PFAM: glycosyl transferase family 9; KEGG: sbc:SbBS512_E4048 lipopolysaccharide heptosyltransferase I; ADP-heptose:LPS heptosyl transferase I complement(107934..108914) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156355 YP_003034369.1 CDS ECBD_0106 NC_012947.1 108918 109964 R catalyzes the transfer of the second heptose to the heptosyl-KDO2 moiety of the lipopolysaccharide inner core; ADP-heptose:LPS heptosyltransferase II complement(108918..109964) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156356 YP_003034370.1 CDS rfaD NC_012947.1 109974 110906 R catalyzes the interconversion between ADP-D-glycero-beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose; ADP-L-glycero-D-mannoheptose-6-epimerase complement(109974..110906) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156357 YP_003034371.1 CDS ECBD_0108 NC_012947.1 111120 112316 D catalyzes the formation of 2-amino-3-oxobutanoate from acetyl-CoA and glycine; 2-amino-3-ketobutyrate coenzyme A ligase 111120..112316 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156358 YP_003034372.1 CDS tdh NC_012947.1 112326 113351 D converts threonine and NAD to 1,2-amino-3-oxobutanoate and NADH; functions in threonine catabolism; L-threonine 3-dehydrogenase 112326..113351 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156359 YP_003034373.1 CDS ECBD_0110 NC_012947.1 113590 114624 D PFAM: glycosyl transferase family 2; glycosyl transferase 113590..114624 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156360 YP_003034374.1 CDS ECBD_0111 NC_012947.1 114611 115570 R PFAM: protein of unknown function DUF610 YibQ; KEGG: sbc:SbBS512_E4043 polysaccharide deacetylase family protein; hypothetical protein complement(114611..115570) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156361 YP_003034375.1 CDS ECBD_0112 NC_012947.1 115574 116833 R PFAM: peptidase M23; hypothetical protein complement(115574..116833) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156362 YP_003034376.1 CDS ECBD_0113 NC_012947.1 116867 118411 R catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate; phosphoglyceromutase complement(116867..118411) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156363 YP_003034377.1 CDS ECBD_0114 NC_012947.1 118656 119087 D PFAM: rhodanese; SMART: Rhodanese domain protein; KEGG: sbc:SbBS512_E4039 rhodanese domain protein; rhodanese 118656..119087 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156364 YP_003034378.1 CDS ECBD_0115 NC_012947.1 119229 119480 D TIGRFAM: glutaredoxin; PFAM: glutaredoxin; KEGG: sfv:SFV_3921 glutaredoxin; glutaredoxin 119229..119480 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156365 YP_003034379.1 CDS ECBD_0116 NC_012947.1 119543 120010 D molecular chaperone that is required for the normal export of envelope proteins out of the cell cytoplasm; in Escherichia coli this proteins forms a homotetramer in the cytoplasm and delivers proteins to be exported to SecA; preprotein translocase subunit SecB 119543..120010 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156366 YP_003034380.1 CDS gpsA NC_012947.1 120010 121029 D catalyzes the NAD(P)H-dependent reduction of glycerol 3-phosphate to glycerone phosphate; NAD(P)H-dependent glycerol-3-phosphate dehydrogenase 120010..121029 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156367 YP_003034381.1 CDS cysE NC_012947.1 121109 121930 D catalyzes the O-acetylation of serine; serine acetyltransferase 121109..121930 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156368 YP_003034382.1 CDS ECBD_0119 NC_012947.1 121983 122456 R member of the SPOUT superfamily of RNA methyltransferases; no methyltransferase activity observed with certain tRNA substrates; tRNA/rRNA methyltransferase YibK complement(121983..122456) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156369 YP_003034383.1 CDS lldD NC_012947.1 122610 123800 R flavin mononucleotide-dependent dehydrogenase; functions in aerobic respiration and also has a role in anaerobic nitrate respiration; L-lactate dehydrogenase complement(122610..123800) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156370 YP_003034384.1 CDS ECBD_0121 NC_012947.1 123797 124573 R represses the lctPRD operon; DNA-binding transcriptional repressor LldR complement(123797..124573) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156371 YP_003034385.1 CDS ECBD_0122 NC_012947.1 124573 126228 R TIGRFAM: L-lactate transport; PFAM: L-lactate permease; KEGG: sbc:SbBS512_E4029 L-lactate permease; L-lactate permease complement(124573..126228) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156372 YP_003034386.1 CDS ECBD_0123 NC_012947.1 126596 131446 R PFAM: YadA domain protein; Hep_Hag repeat-containing protein; Haemagluttinin domain protein; KEGG: ssn:SSON_3803 adhesin; YadA domain protein complement(126596..131446) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156373 YP_003034387.1 CDS ECBD_0124 NC_012947.1 131490 132173 R KEGG: sfv:SFV_3931 inner membrane lipoprotein; inner membrane lipoprotein complement(131490..132173) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156374 YP_003034388.1 CDS ECBD_0125 NC_012947.1 132717 133079 R KEGG: sbc:SbBS512_E4020 hypothetical protein; hypothetical protein complement(132717..133079) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156375 YP_003034389.1 CDS ECBD_0126 NC_012947.1 133364 133573 D KEGG: sfv:SFV_3935 cytoplasmic protein; cytoplasmic protein 133364..133573 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156376 YP_003034390.1 CDS mtlR NC_012947.1 133585 134172 R Acts as a repressor of the mtlAD operon; mannitol repressor protein complement(133585..134172) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156377 YP_003034391.1 CDS ECBD_0128 NC_012947.1 134172 135320 R PFAM: Mannitol dehydrogenase domain; Mannitol dehydrogenase rossman domain; KEGG: elf:LF82_1407 mannitol-1-phosphate 5-dehydrogenase; mannitol-1-phosphate 5-dehydrogenase complement(134172..135320) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156378 YP_003034392.1 CDS ECBD_0129 NC_012947.1 135550 137463 R TIGRFAM: PTS system, mannitol-specific IIC subunit; PFAM: phosphoenolpyruvate-dependent sugar phosphotransferase system EIIA 2; phosphotransferase system EIIC; phosphotransferase system lactose/cellobiose-specific IIB subunit; PTS system, mannitol-specific IIC subunit complement(135550..137463) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156379 YP_003034393.1 CDS ECBD_0130 NC_012947.1 138000 138362 D KEGG: ssn:SSON_3810 hypothetical protein; hypothetical protein 138000..138362 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156380 YP_003034394.1 CDS ECBD_0131 NC_012947.1 138365 139501 D PFAM: secretion protein HlyD; secretion protein HlyD 138365..139501 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156381 YP_003034395.1 CDS ECBD_0132 NC_012947.1 139961 140398 R hypothetical protein complement(139961..140398) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156382 YP_003034396.1 CDS ECBD_0134 NC_012947.1 141105 141566 R KEGG: sbo:SBO_3594 hypothetical protein; hypothetical protein complement(141105..141566) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156384 YP_003034397.1 CDS ECBD_0135 NC_012947.1 141578 142516 R PFAM: RHS protein; KEGG: sbo:SBO_3593 RhsA; RHS protein complement(141578..142516) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156385 YP_003034398.1 CDS ECBD_0136 NC_012947.1 142564 143406 R PFAM: PBS lyase HEAT domain protein repeat-containing protein; KEGG: sei:SPC_1578 hypothetical protein; hypothetical protein complement(142564..143406) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156386 YP_003034399.1 CDS ECBD_0137 NC_012947.1 143427 144299 R KEGG: sbo:SBO_0563 RhsC protein in rhs element; Rhs family protein-like protein complement(143427..144299) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156387 YP_003034400.1 CDS ECBD_0138 NC_012947.1 144528 145136 D PFAM: glutathione S-transferase domain; KEGG: sbc:SbBS512_E4007 glutathione S-transferase; glutathione S-transferase 144528..145136 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156388 YP_003034401.1 CDS ECBD_0139 NC_012947.1 145234 146625 D catalyzes the formation of selenocysteinyl-tRNA(Sec) from seryl-tRNA(Sec) and L-selenophosphate in selenoprotein biosynthesis; selenocysteine synthase 145234..146625 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156389 YP_003034402.1 CDS ECBD_0140 NC_012947.1 146622 148466 D TIGRFAM: selenocysteine-specific translation elongation factor; PFAM: protein synthesis factor GTP-binding; Elongation factor SelB winged helix 3; Elongation factor SelB winged helix 2; elongation factor Tu domain 2 protein; KEGG: elf:LF82_2111 selenocysteine-specific elongation factor; selenocysteinyl-tRNA-specific translation factor 146622..148466 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156390 YP_003034403.1 CDS ECBD_0141 NC_012947.1 148656 149807 D PFAM: iron-containing alcohol dehydrogenase; KEGG: elf:LF82_3344 alcohol dehydrogenase; alcohol dehydrogenase 148656..149807 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156391 YP_003034404.1 CDS ECBD_0142 NC_012947.1 149972 151510 D PFAM: aldehyde dehydrogenase; KEGG: sbc:SbBS512_E4003 aldehyde dehydrogenase B; aldehyde dehydrogenase (NAD(+)) 149972..151510 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156392 YP_003034405.1 CDS ECBD_0143 NC_012947.1 151729 151941 R KEGG: sfv:SFV_3946 hypothetical protein; hypothetical protein complement(151729..151941) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156393 YP_003034406.1 CDS ECBD_0144 NC_012947.1 152055 152378 D hypothetical protein 152055..152378 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156394 YP_003034407.1 CDS ECBD_0145 NC_012947.1 152384 153520 D PFAM: secretion protein HlyD; secretion protein HlyD 152384..153520 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156395 YP_003034408.1 CDS ECBD_0146 NC_012947.1 153517 154491 R PFAM: regulatory protein LysR; LysR substrate-binding; LysR family transcriptional regulator complement(153517..154491) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156396 YP_003034409.1 CDS ECBD_0147 NC_012947.1 154615 155355 D PFAM: MltA-interacting MipA family protein; KEGG: sfv:SFV_3952 outer membrane protein; MltA-interacting MipA family protein 154615..155355 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156397 YP_003034410.1 CDS ECBD_0148 NC_012947.1 155476 157455 R PFAM: protein of unknown function DUF1680; KEGG: sfv:SFV_3953 hypothetical protein; hypothetical protein complement(155476..157455) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156398 YP_003034411.1 CDS ECBD_0149 NC_012947.1 157466 158866 R TIGRFAM: sugar (glycoside-Pentoside-Hexuronide) transporter; KEGG: sfv:SFV_3954 permease; sugar (glycoside-Pentoside-Hexuronide) transporter complement(157466..158866) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156399 YP_003034412.1 CDS ECBD_0150 NC_012947.1 159092 159907 D PFAM: helix-turn-helix- domain containing protein AraC type; AraC protein arabinose-binding/dimerisation; SMART: helix-turn-helix- domain containing protein AraC type; KEGG: sfv:SFV_3955 transcriptional regulator; AraC family transcriptional regulator 159092..159907 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156400 YP_003034413.1 CDS sgbE NC_012947.1 159939 160634 R catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate in the anaerobic catabolism of L-ascorbate; links the arabinose metabolic pathway to the pentose phosphate pathway and allows the bacteria to use arabinose as an energy source; L-ribulose-5-phosphate 4-epimerase complement(159939..160634) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156401 YP_003034414.1 CDS ECBD_0152 NC_012947.1 160628 161488 R L-xylulose 5-phosphate 3-epimerase activity not yet demonstrated; may be involved in the utilization of 2,3-diketo-L-gulonate; L-xylulose 5-phosphate 3-epimerase complement(160628..161488) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156402 YP_003034415.1 CDS sgbH NC_012947.1 161481 162143 R catalyzes the formation of L-xylulose-5-phosphate from 3-keto-L-gulonate-6-phosphate; 3-keto-L-gulonate-6-phosphate decarboxylase complement(161481..162143) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156403 YP_003034416.1 CDS ECBD_0154 NC_012947.1 162140 163636 R PFAM: carbohydrate kinase FGGY; KEGG: sfv:SFV_3959 L-xylulose kinase, cryptic; carbohydrate kinase FGGY complement(162140..163636) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156404 YP_003034417.1 CDS ECBD_0155 NC_012947.1 163640 164626 R TIGRFAM: TRAP dicarboxylate transporter DctP subunit; PFAM: TRAP dicarboxylate transporter- DctP subunit; KEGG: sfv:SFV_3960 solute-binding transporter; TRAP dicarboxylate transporter DctP subunit complement(163640..164626) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156405 YP_003034418.1 CDS ECBD_0156 NC_012947.1 164639 165916 R TIGRFAM: TRAP dicarboxylate transporter DctM subunit; PFAM: TRAP C4-dicarboxylate transporter permease DctM subunit; TRAP dicarboxylate transporter DctM subunit complement(164639..165916) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156406 YP_003034419.1 CDS ECBD_0157 NC_012947.1 165919 166392 R membrane-spanning protein involved in uptake of 2,3-diketo-L-gulonate; part of the tri-partite ATP-independent periplasmic transporter (TRAP) YiaMNO; 2,3-diketo-L-gulonate TRAP transporter small permease protein YiaM complement(165919..166392) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156407 YP_003034420.1 CDS ECBD_0158 NC_012947.1 166510 166977 R PFAM: conserved hypothetical protein; KEGG: efe:EFER_3574 hypothetical protein; hypothetical protein complement(166510..166977) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156408 YP_003034421.1 CDS ECBD_0159 NC_012947.1 166989 167987 R NAD(P)H-dependent; catalyzes the reduction of 2,3-diketo-L-gulonate to 3-keto-L-gulonate; 2,3-diketo-L-gulonate reductase complement(166989..167987) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156409 YP_003034422.1 CDS ECBD_0160 NC_012947.1 168188 169036 D PFAM: transcriptional regulator IclR ; regulatory protein IclR; SMART: regulatory protein IclR; KEGG: sbo:SBO_3582 regulator; transcriptional regulator, IclR family 168188..169036 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156410 YP_003034423.1 CDS ECBD_0161 NC_012947.1 169138 169611 D PFAM: 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: sbo:SBO_3581 hypothetical protein; 4Fe-4S ferredoxin iron-sulfur binding domain protein 169138..169611 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156411 YP_003034424.1 CDS avtA NC_012947.1 169762 171015 R transaminase C; catalyzes transamination of alanine, valine, and 2-aminobutyrate with their respective 2-keto acids; also catalyzes terminal step in valine biosynthesis; valine--pyruvate transaminase complement(169762..171015) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156412 YP_003034425.1 CDS malS NC_012947.1 171193 173223 R periplasmic enzyme that degrades maltodextrins that enter via the outer membrane porin LamB; hydrolyzes alpha-(1,4) glycosidic linkages; contains internal disulfide bonds; periplasmic alpha-amylase precursor complement(171193..173223) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156413 YP_003034426.1 CDS ECBD_0164 NC_012947.1 173543 174367 D PFAM: mannosyl-glycoprotein endo-beta-N-acetylglucosamidase; SMART: Lysozyme subfamily 2; KEGG: sfv:SFV_3970 hypothetical protein; hypothetical protein 173543..174367 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156414 YP_003034427.1 CDS ECBD_0165 NC_012947.1 174563 175741 R PFAM: helix-turn-helix- domain containing protein AraC type; SMART: helix-turn-helix- domain containing protein AraC type; KEGG: sbc:SbBS512_E3953 xylose operon regulatory protein; AraC family transcriptional regulator complement(174563..175741) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156415 YP_003034428.1 CDS ECBD_0166 NC_012947.1 175819 177000 R PFAM: inner-membrane translocator; KEGG: sbc:SbBS512_E3954 D-xylose ABC transporter permease; inner-membrane translocator complement(175819..177000) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156416 YP_003034429.1 CDS xylF NC_012947.1 178595 179587 R periplasmic substrate-binding component of the ATP-dependent xylose transporter; high affinity; D-xylose transporter subunit XylF complement(178595..179587) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157020 YP_003034430.1 CDS ECBD_0169 NC_012947.1 179953 181275 D catalyzes the interconversion of D-xylose to D-xylulose; xylose isomerase 179953..181275 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157021 YP_003034431.1 CDS ECBD_0170 NC_012947.1 181347 182801 D TIGRFAM: xylulokinase; PFAM: carbohydrate kinase FGGY; KEGG: sfv:SFV_3976 xylulokinase; xylulokinase 181347..182801 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157022 YP_003034432.1 CDS ECBD_0171 NC_012947.1 182970 183311 D PFAM: YiaAB two helix domain protein; hypothetical protein 182970..183311 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157023 YP_003034433.1 CDS ECBD_0172 NC_012947.1 183357 183794 D PFAM: YiaAB two helix domain protein; KEGG: sfv:SFV_3978 hypothetical protein; hypothetical protein 183357..183794 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157024 YP_003034434.1 CDS ECBD_0173 NC_012947.1 183836 184831 R PFAM: acyltransferase 3; KEGG: sdy:SDY_4340 hypothetical protein; acyltransferase 3 complement(183836..184831) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157025 YP_003034435.1 CDS ECBD_0174 NC_012947.1 185006 185305 D KEGG: sfv:SFV_3980 lipoprotein; lipoprotein 185006..185305 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157026 YP_003034436.1 CDS glyQ NC_012947.1 185400 186311 D glycine--tRNA ligase alpha chain; GlyRS; class II aminoacyl tRNA synthetase; tetramer of alpha(2)beta(2); catalyzes a two-step reaction; first charging a glycine molecule by linking its carboxyl group to the alpha-phosphate of ATP; second by transfer of the aminoacyl-adenylate to its tRNA; glycyl-tRNA synthetase subunit alpha 185400..186311 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157027 YP_003034437.1 CDS glyS NC_012947.1 186321 188390 D glycine--tRNA ligase beta chain; glyS; class II aminoacyl tRNA synthetase; tetramer of alpha(2)beta(2); catalyzes a two-step reaction; first charging a glycine molecule by linking the carboxyl group to the alpha-phosphate of ATP; second by transfer of the aminoacyl-adenylate to its tRNA; glycyl-tRNA synthetase subunit beta 186321..188390 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157028 YP_003034438.1 CDS ECBD_0177 NC_012947.1 188669 189520 R PFAM: Integrase catalytic region; KEGG: sfv:SFV_3530 IS150 ORF2; Integrase catalytic region complement(188669..189520) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157029 YP_003034439.1 CDS ECBD_0178 NC_012947.1 189517 190038 R KEGG: sfx:S1483 IS103 orf; IS103 orf complement(189517..190038) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157030 YP_003034440.1 CDS ECBD_0179 NC_012947.1 190118 190270 D PFAM: Hok/gef cell toxic protein; KEGG: elf:LF82_1021 protein HokA; small toxic polypeptide 190118..190270 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157031 YP_003034441.1 CDS ECBD_0180 NC_012947.1 190457 190669 R PFAM: Cold-shock protein DNA-binding; SMART: Cold shock protein; KEGG: sea:SeAg_B3864 major cold shock protein; major cold shock protein complement(190457..190669) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157032 YP_003034442.1 CDS ECBD_0181 NC_012947.1 190950 191240 R SMART: helix-turn-helix domain protein; KEGG: sdy:SDY_4348 transcriptional regulator; transcriptional regulator complement(190950..191240) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157033 YP_003034443.1 CDS ECBD_0182 NC_012947.1 191405 191557 D hypothetical protein 191405..191557 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157034 YP_003034444.1 CDS ECBD_0183 NC_012947.1 191674 192384 D KEGG: sdy:SDY_4349 hypothetical protein; hypothetical protein 191674..192384 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157035 YP_003034445.1 CDS ECBD_0184 NC_012947.1 192434 193408 R PFAM: D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding; D-isomer specific 2-hydroxyacid dehydrogenase catalytic region; KEGG: sbc:SbBS512_E3973 2-ketogluconate reductase; D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding complement(192434..193408) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157036 YP_003034446.1 CDS ECBD_0185 NC_012947.1 193512 194171 R PFAM: OmpA/MotB domain protein; 17 kDa surface antigen; outer membrane lipoprotein complement(193512..194171) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157037 YP_003034447.1 CDS ECBD_0186 NC_012947.1 194324 196657 D KEGG: sbc:SbBS512_E3976 biotin sulfoxide reductase; TIGRFAM: molybdopterin guanine dinucleotide-containing S/N-oxide reductase; PFAM: molybdopterin oxidoreductase; molydopterin dinucleotide-binding region; molybdopterin guanine dinucleotide-containing S/N-oxide reductase 194324..196657 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157038 YP_003034448.1 CDS ECBD_0187 NC_012947.1 196626 197066 R PFAM: GCN5-related N-acetyltransferase; KEGG: sbc:SbBS512_E3975 acetyltransferase, GNAT family; hypothetical protein complement(196626..197066) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157039 YP_003034449.1 CDS ECBD_0188 NC_012947.1 197063 197626 R constitutive, catalyzes the hydrolysis of alkylated DNA, releasing 3-methyladenine; 3-methyl-adenine DNA glycosylase I complement(197063..197626) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157040 YP_003034450.1 CDS ECBD_0189 NC_012947.1 197784 198482 D KEGG: sbc:SbBS512_E3978 hypothetical protein; hypothetical protein 197784..198482 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157041 YP_003034451.1 CDS ECBD_0190 NC_012947.1 198711 199919 D TIGRFAM: Oxalate/formate antiporter; PFAM: major facilitator superfamily MFS_1; KEGG: ssn:SSON_3843 resistance protein; Oxalate/formate antiporter 198711..199919 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157042 YP_003034452.1 CDS ECBD_0191 NC_012947.1 200243 201934 D catalyzes the addition of a phosphoethanolamine group to the outer Kdo residue of lipopolysaccharide; phosphoethanolamine transferase 200243..201934 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157043 YP_003034453.1 CDS ECBD_0192 NC_012947.1 203013 204620 D PFAM: extracellular solute-binding protein family 5; KEGG: sfv:SFV_3543 dipeptide transporter; extracellular solute-binding protein family 5 203013..204620 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157045 YP_003034454.1 CDS ECBD_0193 NC_012947.1 204928 205947 D transports peptides consisting of two or three amino acids; dipeptide transporter permease DppB 204928..205947 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157046 YP_003034455.1 CDS ECBD_0194 NC_012947.1 205957 206859 D PFAM: binding-protein-dependent transporters inner membrane component; dipeptide transporter 205957..206859 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157047 YP_003034456.1 CDS dppD NC_012947.1 206870 207853 D DppD and DppF are the ATP-binding components of the ABC dipeptide transporter DppABCDF; dipeptide transporter ATP-binding subunit 206870..207853 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157048 YP_003034457.1 CDS dppF NC_012947.1 207850 208854 D Part of the ABC transporter complex DppABCDF involved in the transport of dipeptides; dipeptide transporter ATP-binding subunit 207850..208854 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157049 YP_003034458.1 CDS ECBD_0197 NC_012947.1 208884 210155 R TIGRFAM: serine transporter; KEGG: ssn:SSON_3851 transporter protein; serine transporter complement(208884..210155) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157050 YP_003034459.1 CDS ECBD_0198 NC_012947.1 210631 210738 D KEGG: sbc:SbBS512_E3995 hypothetical protein; hypothetical protein 210631..210738 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157051 YP_003034460.1 CDS ECBD_0199 NC_012947.1 211114 211221 D KEGG: sbc:SbBS512_E3995 hypothetical protein; hypothetical protein 211114..211221 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157052 YP_003034461.1 CDS ECBD_0200 NC_012947.1 211597 211704 D KEGG: sfl:SF3573 hypothetical protein; hypothetical protein 211597..211704 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157053 YP_003034462.1 CDS ECBD_0201 NC_012947.1 211791 213470 R TIGRFAM: cellulose synthase operon protein YhjU; KEGG: sfv:SFV_3549 hypothetical protein; cellulose synthase operon protein YhjU complement(211791..213470) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157054 YP_003034463.1 CDS ECBD_0202 NC_012947.1 213467 213658 R KEGG: sbc:SbBS512_E3999 cellulose biosynthesis protein BcsF; cellulose biosynthesis protein BcsF complement(213467..213658) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157055 YP_003034464.1 CDS ECBD_0203 NC_012947.1 213655 215214 R TIGRFAM: cellulose biosynthesis protein BcsE; KEGG: sfx:S4199 protease; cellulose biosynthesis protein BcsE complement(213655..215214) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157056 YP_003034465.1 CDS ECBD_0204 NC_012947.1 215499 215687 D KEGG: sfv:SFV_3553 hypothetical protein; hypothetical protein 215499..215687 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157057 YP_003034466.1 CDS ECBD_0205 NC_012947.1 215699 216451 D TIGRFAM: cellulose synthase operon protein YhjQ; PFAM: YhjQ family protein; KEGG: sbc:SbBS512_E3940 YhjQ protein; cell division protein 215699..216451 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157058 YP_003034467.1 CDS bcsA NC_012947.1 216448 219066 D polymerizes uridine 5'-diphosphate glucose to cellulose; acts with BcsB, BcsZ and BcsC in cellulose biosynthesis; cellulose synthase catalytic subunit 216448..219066 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157059 YP_003034468.1 CDS ECBD_0207 NC_012947.1 219113 221416 D binds the cellulose synthase activator, bis-(3'-5') cyclic diguanylic acid (c-di-GMP); cellulose synthase regulator protein 219113..221416 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157060 YP_003034469.1 CDS ECBD_0208 NC_012947.1 221423 222529 D catalyzes the hydrolysis of 1,4-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans; endo-1,4-D-glucanase 221423..222529 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157061 YP_003034470.1 CDS ECBD_0209 NC_012947.1 222511 225984 D cellulose is produced by the multicellular morphotypes of E. coli and Salmonella typhimurium; the cellulose biosynthesis genes bcsA, bcsB, bcsZ and bcsC are constitutively transcribed, however, cellulose synthesis is dependent on the expression of adrA; cellulose synthase subunit BcsC 222511..225984 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157062 YP_003034471.1 CDS ECBD_0210 NC_012947.1 226105 228054 D HmsP in Yersinia pestis plays a role in invasion of epithelial cells; the EAL-domain portion of HmsP from Y. pestis shows phosphodiesterase activity which is required for the inhibition of biofilm formation; inner membrane protein; similar to a phosphodiesterase protein from E. coli; phosphodiesterase 226105..228054 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157063 YP_003034472.1 CDS ECBD_0211 NC_012947.1 228237 229523 D involved in the transport of C4-dicarboxylates across the membrane; C4-dicarboxylate transporter DctA 228237..229523 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157064 YP_003034473.1 CDS ECBD_0212 NC_012947.1 229744 231240 D PFAM: peptidase M16 domain protein; KEGG: sdy:SDY_4547 hypothetical protein; peptidase M16 domain protein 229744..231240 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157065 YP_003034474.1 CDS ECBD_0213 NC_012947.1 231336 232265 R PFAM: PfkB domain protein; PfkB domain protein complement(231336..232265) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157066 YP_003034475.1 CDS ECBD_0214 NC_012947.1 232497 233264 D in Escherichia coli this protein is involved in flagellar function; EAL domain-containing protein 232497..233264 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157067 YP_003034476.1 CDS ECBD_0215 NC_012947.1 233319 235394 D PFAM: AsmA family protein; KEGG: sbo:SBO_3523 hypothetical protein; AsmA family protein 233319..235394 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157068 YP_003034477.1 CDS ECBD_0216 NC_012947.1 235576 236898 R TIGRFAM: metabolite/H+ symporter, major facilitator superfamily (MFS); PFAM: general substrate transporter; major facilitator superfamily MFS_1; KEGG: ssn:SSON_3868 transporter; metabolite/H+ symporter, major facilitator superfamily (MFS) complement(235576..236898) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157069 YP_003034478.1 CDS ECBD_0217 NC_012947.1 237309 238322 R TIGRFAM: ribonuclease; PFAM: ribonuclease BN; KEGG: sbc:SbBS512_E3927 ribonuclease; ribonuclease complement(237309..238322) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157070 YP_003034479.1 CDS ECBD_0218 NC_012947.1 238371 239342 R PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: sbo:SBO_3520 transcriptional regulator LysR-type; LysR family transcriptional regulator complement(238371..239342) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157071 YP_003034480.1 CDS ECBD_0219 NC_012947.1 239790 240392 D PFAM: regulatory protein LuxR; Sigma-70 region 4 type 2; SMART: regulatory protein LuxR; KEGG: sbc:SbBS512_E3925 transcriptional regulator, LuxR family; LuxR family transcriptional regulator 239790..240392 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157072 YP_003034481.1 CDS treF NC_012947.1 240443 242092 R cytoplasmic; catalyzes the hydrolysis of trehalose to glucose; trehalase complement(240443..242092) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157073 YP_003034482.1 CDS ECBD_0221 NC_012947.1 242497 243894 D PFAM: Di-haem cytochrome C peroxidase; KEGG: sfv:SFV_3990 cytochrome C peroxidase; cytochrome-c peroxidase 242497..243894 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157074 YP_003034483.1 CDS ECBD_0222 NC_012947.1 244105 245505 D TIGRFAM: glutamate decarboxylase; PFAM: pyridoxal-dependent decarboxylase; KEGG: sbo:SBO_3516 glutamate decarboxylase isozyme; glutamate decarboxylase 244105..245505 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157075 YP_003034484.1 CDS ECBD_0223 NC_012947.1 245620 245790 D hypothetical protein 245620..245790 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157076 YP_003034485.1 CDS ECBD_0224 NC_012947.1 245875 246699 D regulates genes in response to acid and/or during stationary phase; DNA-binding transcriptional regulator GadX 245875..246699 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157077 YP_003034486.1 CDS ECBD_0225 NC_012947.1 247067 247795 D PFAM: helix-turn-helix- domain containing protein AraC type; SMART: helix-turn-helix- domain containing protein AraC type; KEGG: sfv:SFV_3987 AraC-type regulatory protein; AraC family transcriptional regulator 247067..247795 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157078 YP_003034487.1 CDS ECBD_0226 NC_012947.1 248158 251271 R TIGRFAM: transporter hydrophobe/amphiphile efflux-1 (HAE1) family; PFAM: acriflavin resistance protein; KEGG: sdy:SDY_3537 transporter permease; transporter hydrophobe/amphiphile efflux-1 (HAE1) family complement(248158..251271) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157079 YP_003034488.1 CDS ECBD_0227 NC_012947.1 251296 252453 R with MdtF and tolC is involved in resistance to rhodamine 6G, erythromycin, doxorubicin and other compounds; multidrug efflux system protein MdtE complement(251296..252453) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157080 YP_003034489.1 CDS ECBD_0228 NC_012947.1 252513 252791 D hypothetical protein 252513..252791 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157081 YP_003034490.1 CDS ECBD_0229 NC_012947.1 252792 253319 R KEGG: sfv:SFV_3526 hypothetical protein; LuxR family transcriptional regulator complement(252792..253319) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157082 YP_003034491.1 CDS ECBD_0230 NC_012947.1 254118 254690 R KEGG: sbc:SbBS512_E3909 HdeD protein; acid-resistance membrane protein complement(254118..254690) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157083 YP_003034492.1 CDS ECBD_0231 NC_012947.1 254945 255277 D inactive form; acid-resistance protein 254945..255277 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157084 YP_003034493.1 CDS hdeB NC_012947.1 255381 255719 D KEGG: sfx:S4224 acid-resistance protein; acid-resistance protein 255381..255719 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157085 YP_003034494.1 CDS ECBD_0233 NC_012947.1 255783 256430 D inner membrane protein involved in cell density-dependent acid resistance; part of the acid fitness island (AFI) of E. coli; Mg(2+) transport ATPase 255783..256430 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157086 YP_003034495.1 CDS ECBD_0234 NC_012947.1 256472 257002 R PFAM: regulatory protein LuxR; SMART: regulatory protein LuxR; LuxR family transcriptional regulator complement(256472..257002) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157087 YP_003034496.1 CDS ECBD_0235 NC_012947.1 257158 257724 R TIGRFAM: outer membrane lipoprotein, Slp family; PFAM: outer membrane lipoprotein Slp; KEGG: sbc:SbBS512_E3904 outer membrane lipoprotein, Slp family; outer membrane lipoprotein, Slp family complement(257158..257724) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157088 YP_003034497.1 CDS ECBD_0236 NC_012947.1 257972 259195 R KEGG: sfv:SFV_3519 hypothetical protein; hypothetical protein complement(257972..259195) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157089 YP_003034498.1 CDS ECBD_0237 NC_012947.1 259824 260249 R KEGG: sbc:SbBS512_E3818 arsenate reductase; TIGRFAM: arsenate reductase; PFAM: arsenate reductase; arsenate reductase complement(259824..260249) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157090 YP_003034499.1 CDS ECBD_0238 NC_012947.1 260262 261551 R TIGRFAM: arsenical pump membrane protein; PFAM: Arsenical pump membrane protein; citrate transporter; KEGG: sbo:SBO_3500 arsenical pump membrane protein; arsenical pump membrane protein complement(260262..261551) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157091 YP_003034500.1 CDS ECBD_0239 NC_012947.1 261605 261958 R regulates the expression of of the arsRBC involved in resistance to arsenic; DNA-binding transcriptional repressor ArsR complement(261605..261958) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157092 YP_003034501.1 CDS ECBD_0240 NC_012947.1 262497 262670 R KEGG: efe:EFER_3494 hypothetical protein; hypothetical protein complement(262497..262670) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157093 YP_003034502.1 CDS ECBD_0241 NC_012947.1 262835 264187 R catalyzes the reduction of 2 glutathione to glutathione disulfide; maintains high levels of reduced glutathione in the cytosol; involved in redox regulation and oxidative defense; glutathione reductase complement(262835..264187) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157094 YP_003034503.1 CDS ECBD_0242 NC_012947.1 264259 265101 R PFAM: protein of unknown function DUF519; KEGG: sbo:SBO_3497 hypothetical protein; hypothetical protein complement(264259..265101) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157095 YP_003034504.1 CDS ECBD_0243 NC_012947.1 265304 267346 D PFAM: peptidase M3A and M3B thimet/oligopeptidase F; oligopeptidase A 265304..267346 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157096 YP_003034505.1 CDS ECBD_0244 NC_012947.1 267354 268106 D predicted SAM-dependent methyltransferase; methyltransferase 267354..268106 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157097 YP_003034506.1 CDS ECBD_0245 NC_012947.1 268155 269624 R member of the POT family of peptide transporters; probable proton-dependent peptide transporter function; inner membrane transporter YhiP complement(268155..269624) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157098 YP_003034507.1 CDS ECBD_0246 NC_012947.1 269942 270376 R PFAM: UspA domain protein; KEGG: cko:CKO_04943 hypothetical protein; UspA domain protein complement(269942..270376) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157099 YP_003034508.1 CDS ECBD_0247 NC_012947.1 270767 271102 D ppGpp-dependent, membrane associated, stress protein produced under conditions of nutrient deprivation, osmotic shock and oxidative stress; universal stress protein UspB 270767..271102 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159320 YP_003034509.1 CDS ECBD_0248 NC_012947.1 271346 272845 R PFAM: phosphate transporter; KEGG: sdy:SDY_3569 low-affinity phosphate transport; phosphate transporter complement(271346..272845) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159321 YP_003034510.1 CDS ECBD_0249 NC_012947.1 273077 274279 D PFAM: HI0933 family protein; FAD-dependent pyridine nucleotide-disulphide oxidoreductase; FAD dependent oxidoreductase; KEGG: sbc:SbBS512_E3830 pyridine nucleotide-disulfide oxidoreductase family protein; HI0933 family protein 273077..274279 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159322 YP_003034511.1 CDS ECBD_0250 NC_012947.1 274594 275646 R hypothetical protein complement(274594..275646) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159323 YP_003034512.1 CDS ECBD_0251 NC_012947.1 276029 277636 D KEGG: sfl:SF3505 hypothetical protein; hypothetical protein 276029..277636 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159324 YP_003034513.1 CDS ECBD_0252 NC_012947.1 277898 279520 D KEGG: sfx:S4259 hypothetical protein; hypothetical protein 277898..279520 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159325 YP_003034514.1 CDS ECBD_0253 NC_012947.1 279886 280953 D PFAM: secretion protein HlyD; KEGG: sfv:SFV_3488 hypothetical protein; secretion protein HlyD 279886..280953 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159326 YP_003034515.1 CDS ECBD_0254 NC_012947.1 280950 283685 D PFAM: ABC transporter; ABC-2 type transporter; SMART: ATPase AAA; KEGG: sbc:SbBS512_E3836 ABC transporter ATP binding protein; ABC transporter 280950..283685 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159327 YP_003034516.1 CDS ECBD_0255 NC_012947.1 283685 284809 D PFAM: ABC transporter; KEGG: sfv:SFV_3486 transporter; ABC transporter 283685..284809 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159328 YP_003034517.1 CDS ECBD_0256 NC_012947.1 284974 286110 R PFAM: transposase IS4 family protein; KEGG: ssn:SSON_0260 receptor; transposase IS4 family protein complement(284974..286110) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159329 YP_003034518.1 CDS ECBD_0257 NC_012947.1 286356 286601 R KEGG: sei:SPC_1578 hypothetical protein; hypothetical protein complement(286356..286601) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159330 YP_003034519.1 CDS ECBD_0258 NC_012947.1 286706 287089 R hypothetical protein complement(286706..287089) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159331 YP_003034520.1 CDS ECBD_0259 NC_012947.1 287061 291296 R TIGRFAM: hypothetical protein; PFAM: RHS protein; YD repeat-containing protein; hypothetical protein complement(287061..291296) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159332 YP_003034521.1 CDS ECBD_0260 NC_012947.1 291499 291900 R Inhibits transcription at high concentrations of nickel; nickel responsive regulator complement(291499..291900) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159333 YP_003034522.1 CDS nikE NC_012947.1 291906 292712 R with NikABCD is involved with nickel transport into the cell; nickel transporter ATP-binding protein NikE complement(291906..292712) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159334 YP_003034523.1 CDS nikD NC_012947.1 292709 293473 R with NikABCE is involved in nickel transport into the cell; nickel transporter ATP-binding protein NikD complement(292709..293473) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159335 YP_003034524.1 CDS nikC NC_012947.1 293473 294306 R with NikABDE is involved in nickel transport into the cell; nickel transporter permease NikC complement(293473..294306) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159336 YP_003034525.1 CDS ECBD_0264 NC_012947.1 294303 295247 R with NikACDE is involved in nickel transport into the cell; nickel transporter permease NikB complement(294303..295247) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159337 YP_003034526.1 CDS ECBD_0265 NC_012947.1 295247 296821 R TIGRFAM: nickel ABC transporter periplasmic nickel-binding protein; PFAM: extracellular solute-binding protein family 5; KEGG: sfx:S4269 periplasmic binding protein for nickel; nickel ABC transporter periplasmic nickel-binding protein complement(295247..296821) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159338 YP_003034527.1 CDS ECBD_0266 NC_012947.1 296932 297519 R PFAM: 4'-phosphopantetheinyl transferase; KEGG: sbo:SBO_3472 holo-(acyl carrier protein) synthase 2; holo-(acyl carrier protein) synthase 2 complement(296932..297519) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159339 YP_003034528.1 CDS ECBD_0267 NC_012947.1 297574 298623 R PFAM: protein of unknown function UPF0118; hypothetical protein complement(297574..298623) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159340 YP_003034529.1 CDS ECBD_0268 NC_012947.1 298722 299972 D PFAM: major facilitator superfamily MFS_1; KEGG: sbc:SbBS512_E3849 transporter major facilitator family; major facilitator superfamily transporter 298722..299972 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159341 YP_003034530.1 CDS ECBD_0269 NC_012947.1 299976 300533 R KEGG: sdy:SDY_3623 hypothetical protein; hypothetical protein complement(299976..300533) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159342 YP_003034531.1 CDS ECBD_0270 NC_012947.1 300606 301271 R TIGRFAM: conserved hypothetical protein; PFAM: protein of unknown function DUF165; KEGG: sbc:SbBS512_E3851 hypothetical protein; hypothetical protein complement(300606..301271) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159343 YP_003034532.1 CDS ECBD_0271 NC_012947.1 301492 301737 D TusA; transfers sulfur to TusBCD complex; involved in thiouridation of U34 position of some tRNAs; sulfur transfer protein SirA 301492..301737 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159344 YP_003034533.1 CDS zntA NC_012947.1 301839 304037 R P-type ATPase involved in the export of lead, cadmium, zinc and mercury; zinc/cadmium/mercury/lead-transporting ATPase complement(301839..304037) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159345 YP_003034534.1 CDS ECBD_0273 NC_012947.1 304111 304737 R PFAM: YhhN family protein; KEGG: sbc:SbBS512_E3854 hypothetical protein; YhhN family protein complement(304111..304737) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159346 YP_003034535.1 CDS ECBD_0274 NC_012947.1 304878 305237 D KEGG: sfv:SFV_3470 receptor; receptor 304878..305237 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159347 YP_003034536.1 CDS ECBD_0275 NC_012947.1 305240 305509 R PFAM: protein of unknown function DUF1145; KEGG: sdy:SDY_3617 hypothetical protein; hypothetical protein complement(305240..305509) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159348 YP_003034537.1 CDS rsmD NC_012947.1 305499 306095 R catalyzes the methylation of 16S rRNA at position G966; 16S rRNA m(2)G966-methyltransferase complement(305499..306095) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159349 YP_003034538.1 CDS ECBD_0277 NC_012947.1 306245 307738 D signal recognition protein receptor; functions in the targeting and insertion of membrane proteins; cell division protein FtsY 306245..307738 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159350 YP_003034539.1 CDS ECBD_0278 NC_012947.1 307741 308409 D ATP-binding protein of an ATP-binding cassette transporter; when bound to FtsX, FtsEX localizes to the cell division site and plays a role in the assembly or stability of the septal ring under low-salt growth conditions; cell division protein FtsE 307741..308409 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159351 YP_003034540.1 CDS ftsX NC_012947.1 308402 309460 D ABC transporter membrane protein; cell division protein FtsX 308402..309460 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159352 YP_003034541.1 CDS ECBD_0280 NC_012947.1 309705 310559 D binds with the catalytic core of RNA polymerase to produce the holoenzyme; this sigma factor is responsible for the expression of heat shock promoters; RNA polymerase factor sigma-32 309705..310559 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159353 YP_003034542.1 CDS ECBD_0281 NC_012947.1 310830 311933 D PFAM: extracellular ligand-binding receptor; KEGG: sfv:SFV_3463 Leu/Ile/Val-binding protein precursor; extracellular ligand-binding receptor 310830..311933 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159354 YP_003034543.1 CDS ECBD_0282 NC_012947.1 312121 312504 R PFAM: GCN5-related N-acetyltransferase; KEGG: sdy:SDY_3610 hypothetical protein; GCN5-related N-acetyltransferase complement(312121..312504) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159355 YP_003034544.1 CDS ECBD_0283 NC_012947.1 312928 314037 D PFAM: extracellular ligand-binding receptor; KEGG: sbc:SbBS512_E3864 high-affinity branched-chain amino acid ABC transporter periplasmic leucine-specific-binding protein LivK; extracellular ligand-binding receptor 312928..314037 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159356 YP_003034545.1 CDS ECBD_0284 NC_012947.1 314085 315011 D LivHMGF is the membrane component of the LIV-I/LS branched-chain amino acid transporter; branched-chain amino acid transporter permease subunit LivH 314085..315011 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159357 YP_003034546.1 CDS livM NC_012947.1 315008 316285 D Part of the ABC transporter complex LivFGHMJ and LivFGHMK involved in the high-affinity transport of branched-chain amino acids; LivFGHMK is specific for the transport of leucine, while LivFGHMJ is a transporter for leucine, isoleucine, and valine; leucine/isoleucine/valine transporter permease subunit 315008..316285 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159358 YP_003034547.1 CDS livG NC_012947.1 316282 317049 D Part of the ABC transporter complexes LivFGHMJ and LivFGHMK involved in the high-affinity transport of branched-chain amino acids; LivFGHMK is specific for the transport of leucine, while LivFGHMJ is a transporter for leucine, isoleucine, and valine; leucine/isoleucine/valine transporter ATP-binding subunit 316282..317049 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159359 YP_003034548.1 CDS livF NC_012947.1 317051 317764 D with LivGHMJ and LivGHMK is part of the high-affinity branched-chain amino acid transporter; LivFGHMK is specific for the transport of leucine, while LivFGHMJ is a transporter for leucine, isoleucine, and valine; leucine/isoleucine/valine transporter ATP-binding subunit 317051..317764 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159360 YP_003034549.1 CDS ECBD_0288 NC_012947.1 318163 319479 D with UgpACE is involved in the uptake of glycerol-3-phosphate; glycerol-3-phosphate transporter periplasmic binding protein 318163..319479 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159361 YP_003034550.1 CDS ECBD_0289 NC_012947.1 319577 320464 D with UgpEC is involved in the uptake of glycerol-3-phosphate; glycerol-3-phosphate transporter permease 319577..320464 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159362 YP_003034551.1 CDS ECBD_0290 NC_012947.1 320461 321306 D with UgpABC is involved in uptake of glycerol-3-phosphate; glycerol-3-phosphate transporter membrane protein 320461..321306 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159363 YP_003034552.1 CDS ugpC NC_012947.1 321308 322378 D part of the UgpABCE glycerol-3-phosphate uptake system; glycerol-3-phosphate transporter ATP-binding subunit 321308..322378 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159364 YP_003034553.1 CDS ugpQ NC_012947.1 322375 323118 D hydrolyzes diesters during transport at the inner face of the cytoplasmic membrane to glycerol-3-phosphate and alcohol; induced when cells are starved for inorganic phosphate; cytoplasmic glycerophosphodiester phosphodiesterase 322375..323118 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159365 YP_003034554.1 CDS ECBD_0293 NC_012947.1 323105 323545 R KEGG: sfl:SF3465 hypothetical protein; hypothetical protein complement(323105..323545) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159366 YP_003034555.1 CDS ggt NC_012947.1 323665 325407 D periplasmic enzyme; post-translationally processed into two subunits which are required for wild-type enzyme activity; cleaves the gammaglutamyl linkages of compounds such as glutathione and transfer the gammaglutamyl group to other amino acids and peptides; gamma-glutamyltranspeptidase 323665..325407 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159367 YP_003034556.1 CDS ECBD_0295 NC_012947.1 325445 325729 R KEGG: sbc:SbBS512_E3877 hypothetical protein; hypothetical protein complement(325445..325729) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159368 YP_003034557.1 CDS ECBD_0296 NC_012947.1 325929 326084 R hypothetical protein complement(325929..326084) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159369 YP_003034558.1 CDS ECBD_0298 NC_012947.1 326730 327005 R PFAM: Insertion element protein; KEGG: hdu:HD1971 insertion element IS1 1/2/3/5/6 protein InsA; Insertion element protein complement(326730..327005) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159371 YP_003034559.1 CDS ECBD_0299 NC_012947.1 327034 327450 R KEGG: elf:LF82_3698 uncharacterized protein YrhA; hypothetical protein complement(327034..327450) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159372 YP_003034560.1 CDS ECBD_0300 NC_012947.1 327447 328625 R TIGRFAM: type VI secretion system effector, Hcp1 family; PFAM: protein of unknown function DUF796; HNH endonuclease; KEGG: sbc:SbBS512_E3883 HNH endonuclease domain protein; type VI secretion system effector, Hcp1 family complement(327447..328625) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159373 YP_003034561.1 CDS ECBD_0301 NC_012947.1 328862 329350 R YhhY; regulated by the fur regulator; unknown function; acetyltransferase YhhY complement(328862..329350) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159374 YP_003034562.1 CDS ECBD_0302 NC_012947.1 329683 330720 D PFAM: oxidoreductase domain protein; oxidoreductase domain; dehydrogenase 329683..330720 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159375 YP_003034563.1 CDS ECBD_0303 NC_012947.1 330843 331538 D PFAM: pirin; Cupin 2 conserved barrel domain protein; KEGG: sbc:SbBS512_E3886 pirin family protein; pirin 330843..331538 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159376 YP_003034564.1 CDS ECBD_0304 NC_012947.1 331535 331684 R hypothetical protein complement(331535..331684) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159377 YP_003034565.1 CDS ECBD_0305 NC_012947.1 331762 332757 D PFAM: periplasmic binding protein/LacI transcriptional regulator; regulatory protein LacI; SMART: regulatory protein LacI; KEGG: efe:EFER_3414 DNA-binding transcriptional repressor; LacI family transcriptional regulator 331762..332757 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159378 YP_003034566.1 CDS gntK NC_012947.1 332935 333423 D thermoresistant; catalyzes the formation of 6-phospho-D-gluconate from gluconate; gluconate kinase 1 332935..333423 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159379 YP_003034567.1 CDS ECBD_0307 NC_012947.1 333427 334767 D TIGRFAM: gluconate transporter; PFAM: gluconate transporter; citrate transporter; KEGG: sdy:SDY_3587 gluconate transporter low affinity GNT 1 system; low affinity gluconate transporter 333427..334767 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159380 YP_003034568.1 CDS ECBD_0308 NC_012947.1 334824 335417 R YhgN; Ec0197; hydrolyzes nucleoside triphosphates with an O6 atom-containing purine base to nucleoside monophosphate and pyrophosphate; seems to play a role in the elimination of aberrant purine-derived nucleotides containing the 6-keto group; shows preference for deoxyinosine triphosphate and xanthosine triphosphate over standard nucleoside triphosphates (dATP, dCTP, dGTP, and dTTP); dITP- and XTP- hydrolase complement(334824..335417) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159381 YP_003034569.1 CDS ECBD_0309 NC_012947.1 335610 336713 D catalyzes the formation of 4-aspartyl phosphate from aspartate 4-semialdehyde; aspartate-semialdehyde dehydrogenase 335610..336713 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159382 YP_003034570.1 CDS ECBD_0310 NC_012947.1 336986 339172 D catalyzes the transfer of a segment of a 1,4-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain; glycogen branching enzyme 336986..339172 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159383 YP_003034571.1 CDS ECBD_0311 NC_012947.1 339169 341142 D catalyzes the hydrolysis of the alpha-1,6-glucosidic linkages in partially depolymerized glycogen; glycogen debranching enzyme 339169..341142 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159384 YP_003034572.1 CDS glgC NC_012947.1 341160 342455 D catalyzes the formation of ADP-glucose and diphosphate from ATP and alpha-D-glucose 1-phosphate; glucose-1-phosphate adenylyltransferase 341160..342455 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159385 YP_003034573.1 CDS glgA NC_012947.1 342455 343888 D catalyzes the formation of alpha-1,4-glucan chains from ADP-glucose; glycogen synthase 342455..343888 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159386 YP_003034574.1 CDS ECBD_0314 NC_012947.1 343907 346354 D KEGG: sfv:SFV_3437 glycogen phosphorylase; TIGRFAM: glycogen/starch/alpha-glucan phosphorylase; PFAM: glycosyl transferase family 35; glycogen/starch/alpha-glucan phosphorylase 343907..346354 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159387 YP_003034575.1 CDS ECBD_0315 NC_012947.1 346483 346764 D KEGG: sfv:SFV_3434 hypothetical protein; hypothetical protein 346483..346764 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159388 YP_003034576.1 CDS glpD NC_012947.1 346970 348475 R in Escherichia coli this homodimeric enzyme is expressed under aerobic conditions; anaerobic expression is repressed by the arcAB system; converts sn-glycerol-3-phosphate and ubiquinone-8 to dihydroxy acetone phosphate and ubiquinol-8; associates with the cytoplasmic membrane; glycerol-3-phosphate dehydrogenase complement(346970..348475) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159389 YP_003034577.1 CDS glpE NC_012947.1 348665 348991 D belongs to rhodanese family; thiosulfate thiotransferase; in Escherichia coli this enzyme catalyzes the formation of thiocyanate from thiosulfate and cyanide with low efficiency; contains an active site cysteine; thiosulfate sulfurtransferase 348665..348991 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159390 YP_003034578.1 CDS ECBD_0318 NC_012947.1 349036 349866 D protease responsible for the cleavage between Ser and Asp residues of proteins in regions of high local hydrophilicity; intramembrane serine protease GlpG 349036..349866 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159391 YP_003034579.1 CDS ECBD_0319 NC_012947.1 349883 350641 D represses the glpD, glpFK, glpTQ, and glpACB operons involved in glycerol-3-phosphate metabolism; DNA-binding transcriptional repressor GlpR 349883..350641 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159392 YP_003034580.1 CDS ECBD_0320 NC_012947.1 350623 352221 R PFAM: regulator of RNA terminal phosphate cyclase; sigma-54 factor interaction domain-containing protein; ATPase associated with various cellular activities AAA_5; SMART: ATPase AAA; KEGG: sfv:SFV_3429 2-component regulator; Sigma 54 interacting domain protein complement(350623..352221) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159393 YP_003034581.1 CDS ECBD_0321 NC_012947.1 352410 353636 D PFAM: protein of unknown function UPF0027; hypothetical protein 352410..353636 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159394 YP_003034582.1 CDS ECBD_0322 NC_012947.1 353640 354656 D catalyzes the conversion of terminal3'-phosphate of RNA to the 2',3'-cyclicphosphodiester; RNA 3'-terminal-phosphate cyclase 353640..354656 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159395 YP_003034583.1 CDS ECBD_0325 NC_012947.1 356294 357499 R PFAM: major facilitator superfamily MFS_1; KEGG: seh:SeHA_C4724 tetracycline resistance protein, class B; major facilitator superfamily MFS_1 complement(356294..357499) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159398 YP_003034584.1 CDS ECBD_0326 NC_012947.1 357581 358204 D PFAM: Tetracyclin repressor domain protein; regulatory protein TetR; KEGG: apa:APP7_1823 tetracycline repressor protein TetR; TetR family transcriptional regulator 357581..358204 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159399 YP_003034585.1 CDS ECBD_0328 NC_012947.1 360950 363343 D KEGG: ssn:SSON_3549 maltodextrin phosphorylase; TIGRFAM: glycogen/starch/alpha-glucan phosphorylase; PFAM: glycosyl transferase family 35; glycogen/starch/alpha-glucan phosphorylase 360950..363343 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158908 YP_003034586.1 CDS malQ NC_012947.1 363353 365437 D amylomaltase; acts to release glucose from maltodextrins; 4-alpha-glucanotransferase 363353..365437 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155935 YP_003034587.1 CDS ECBD_0330 NC_012947.1 365548 366864 R TIGRFAM: gluconate transporter; PFAM: gluconate transporter; citrate transporter; KEGG: sbc:SbBS512_E3796 high-affinity gluconate transporter; gluconate transporter complement(365548..366864) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155936 YP_003034588.1 CDS ECBD_0331 NC_012947.1 367224 367799 R cytoplasmic protein that may be involved in the utilization of double-stranded DNA as a carbon source; DNA uptake protein complement(367224..367799) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155937 YP_003034589.1 CDS ECBD_0332 NC_012947.1 367858 368541 R involved in high-affinity gluconate transport; gluconate periplasmic binding protein complement(367858..368541) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155938 YP_003034590.1 CDS ECBD_0333 NC_012947.1 368579 369349 D Shows carboxylesterase activity with a preference for short chain fatty acid esters; involved in pimeloyl-CoA synthesis; carboxylesterase BioH 368579..369349 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155939 YP_003034591.1 CDS ECBD_0334 NC_012947.1 369378 370256 R PFAM: transposase YhgA family protein; KEGG: sbc:SbBS512_E3792 transposase, YhgA; hypothetical protein complement(369378..370256) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155940 YP_003034592.1 CDS ECBD_0335 NC_012947.1 370459 370695 R PFAM: protein of unknown function DUF1920; KEGG: sdy:SDY_3666 hypothetical protein; hypothetical protein complement(370459..370695) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155941 YP_003034593.1 CDS feoB NC_012947.1 370695 373016 R cytoplasmic membrane ferrous uptake system permease; mutations disrupt the ability of Escherichia coli to take up ferrous iron; GTP-binding protein which requires GTP for efficient iron(II) uptake; ferrous iron transporter B complement(370695..373016) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155942 YP_003034594.1 CDS feoA NC_012947.1 373033 373260 R PFAM: FeoA family protein; KEGG: sdy:SDY_3668 ferrous iron transporter A; ferrous iron transporter A complement(373033..373260) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155943 YP_003034595.1 CDS ECBD_0338 NC_012947.1 373717 376038 R PFAM: RNA binding S1 domain protein; SMART: Resolvase RNase H domain protein fold; RNA binding S1 domain protein; KEGG: sbc:SbBS512_E3786 transcriptional accessory protein; RNA binding S1 domain protein complement(373717..376038) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155944 YP_003034596.1 CDS greB NC_012947.1 376135 376611 R necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites; arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus; transcription elongation factor GreB complement(376135..376611) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155945 YP_003034597.1 CDS ompR NC_012947.1 376839 377558 D part of two-component system EnvZ/OmpR; regulates transcription of outer membrane porin genes ompC/F; under high osmolarity EnvZ functions as kinase/phosphotransferase and phosphorylates OmpR; the result is increased expression of ompC and repression of ompF; also functions in regulation of other genes; forms dimers upon phosphorylation; osmolarity response regulator 376839..377558 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155946 YP_003034598.1 CDS envZ NC_012947.1 377555 378907 D membrane-localized osmosensor; histidine kinase; in high osmolarity EnvZ autophosphorylates itself and transfers phosphoryl group to OmpR; osmolarity sensor protein 377555..378907 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155947 YP_003034599.1 CDS ECBD_0342 NC_012947.1 378983 380605 R PEP carboxykinase; PEP carboxylase; PEPCK; catalyzes the phosphorylation and decarboxylation of oxaloacetate to form phosphoenolpyruvate using ATP; phosphoenolpyruvate carboxykinase complement(378983..380605) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155948 YP_003034600.1 CDS ECBD_0343 NC_012947.1 380850 380978 D KEGG: sbc:SbBS512_E3781 hypothetical protein; hypothetical protein 380850..380978 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155949 YP_003034601.1 CDS ECBD_0344 NC_012947.1 380984 382708 D KEGG: sbc:SbBS512_E3780 hypothetical protein; hypothetical protein 380984..382708 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155950 YP_003034602.1 CDS hslO NC_012947.1 382771 383649 R becomes active under oxidative stress; four conserved cysteines bind a zinc atom when they are in the reduced state and the enzyme is inactive; oxidative stress results in oxidized cysteines, release of zinc, and binding of Hsp33 to aggregation-prone proteins; forms dimers and higher order oligomers; Hsp33-like chaperonin complement(382771..383649) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155951 YP_003034603.1 CDS ECBD_0346 NC_012947.1 383674 384075 R PFAM: RNA-binding S4 domain protein; SMART: RNA-binding S4 domain protein; KEGG: sdy:SDY_3678 ribosome-associated heat shock protein HSP15; ribosome-associated heat shock protein Hsp15 complement(383674..384075) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155952 YP_003034604.1 CDS ECBD_0347 NC_012947.1 384086 384754 R TIGRFAM: HAD-superfamily hydrolase, subfamily IA, variant 3; PFAM: haloacid dehalogenase; KEGG: sfv:SFV_3404 phosphatase; HAD-superfamily hydrolase, subfamily IA, variant 3 complement(384086..384754) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155953 YP_003034605.1 CDS ECBD_0348 NC_012947.1 384819 386942 R PFAM: Intracellular growth attenuator IgaA; KEGG: sfv:SFV_3403 dehydrogenase; Intracellular growth attenuator IgaA complement(384819..386942) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155954 YP_003034606.1 CDS ECBD_0349 NC_012947.1 386951 387070 R KEGG: sbc:SbBS512_E3775 hypothetical protein; hypothetical protein complement(386951..387070) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155955 YP_003034607.1 CDS nudE NC_012947.1 387274 387834 D ADP-sugar pyrophosphatase; catalyzes the formation of D-ribose 5-phosphate from ADP-ribose; can also act on ADP-mannose and ADP-glucose; ADP-ribose diphosphatase NudE 387274..387834 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155956 YP_003034608.1 CDS mrcA NC_012947.1 388002 390554 R bifunctional murein transglycosylase/murein transpeptidase; penicillin-binding protein 1A; involved in the synthesis of cross-linked peptidoglycan from the lipid intermediates in cell wall formation; penicillin-insensitive transglycosylase catalyzes the formation of linear glycan strands and the penicillin-sensitive transpeptidase catalyzes the cross-linking of the peptide subunits; peptidoglycan synthetase complement(388002..390554) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155957 YP_003034609.1 CDS ECBD_0352 NC_012947.1 390674 391453 D KEGG: ssn:SSON_3526 hypothetical protein; hypothetical protein 390674..391453 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155958 YP_003034610.1 CDS ECBD_0353 NC_012947.1 391453 391992 D PFAM: fimbrial assembly family protein; KEGG: sfl:SF3412 hypothetical protein; fimbrial assembly family protein 391453..391992 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155959 YP_003034611.1 CDS ECBD_0354 NC_012947.1 391976 392416 D KEGG: sfv:SFV_3398 hypothetical protein; hypothetical protein 391976..392416 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155960 YP_003034612.1 CDS ECBD_0355 NC_012947.1 392406 392810 D KEGG: sdy:SDY_3687 hypothetical protein; hypothetical protein 392406..392810 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155961 YP_003034613.1 CDS hofQ NC_012947.1 392722 393960 D outer membrane porin probably involved in uptake of extracellular double-stranded DNA; similar to outer membrane competence protein ComE from Haemophilus influenzae and outer membrane protein PilQ involved in type IV pilus production from Pseudomonas aeruginosa; outer membrane porin HofQ 392722..393960 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155962 YP_003034614.1 CDS aroK NC_012947.1 394205 394882 D type I enzyme similar to type II but differentially regulated; major shikimate kinase in fully repressed cells; catalyzes the formation of shikimate 3-phosphate from shikimate in aromatic amino acid biosynthesis; shikimate kinase I 394205..394882 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155963 YP_003034615.1 CDS aroB NC_012947.1 394939 396027 D catalyzes the formation of 3-dehydroquinate from 3-deoxy-arabino-heptulonate 7-phosphate; functions in aromatic amino acid biosynthesis; 3-dehydroquinate synthase 394939..396027 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155964 YP_003034616.1 CDS ECBD_0359 NC_012947.1 396119 397405 D PFAM: sporulation domain protein; KEGG: sbo:SBO_3375 hypothetical protein; hypothetical protein 396119..397405 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155965 YP_003034617.1 CDS ECBD_0360 NC_012947.1 397512 398348 D KEGG: sbc:SbBS512_E3764 DNA adenine methylase; TIGRFAM: DNA adenine methylase; PFAM: D12 class N6 adenine-specific DNA methyltransferase; DNA adenine methylase 397512..398348 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155966 YP_003034618.1 CDS ECBD_0361 NC_012947.1 398366 399043 D catalyzes the interconversion of D-ribulose 5-phosphate to xylulose 5-phosphate; ribulose-phosphate 3-epimerase 398366..399043 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155967 YP_003034619.1 CDS ECBD_0362 NC_012947.1 399036 399794 D catalyzes the dephosphorylation of 2-phosphoglycolate to form glycolate and phosphate; phosphoglycolate phosphatase 399036..399794 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155968 YP_003034620.1 CDS ECBD_0363 NC_012947.1 399787 400791 D catalyzes a two-step reaction, first charging a tryptophan molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; tryptophanyl-tRNA synthetase 399787..400791 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155969 YP_003034621.1 CDS ECBD_0364 NC_012947.1 401081 401986 D hypothetical protein 401081..401986 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155970 YP_003034622.1 CDS ECBD_0365 NC_012947.1 402003 402365 D KEGG: sbc:SbBS512_E3759 hypothetical protein; hypothetical protein 402003..402365 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155971 YP_003034623.1 CDS ECBD_0366 NC_012947.1 402449 403612 D PFAM: alanine racemase domain protein; KEGG: sbc:SbBS512_E3758 alanine racemase family; alanine racemase domain protein 402449..403612 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155972 YP_003034624.1 CDS ECBD_0367 NC_012947.1 403612 404838 D PFAM: phosphopentomutase domain protein; metalloenzyme domain protein; KEGG: elf:LF82_3289 uncharacterized protein YhfW; mutase 403612..404838 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155973 YP_003034625.1 CDS ECBD_0368 NC_012947.1 404835 405713 D phosphotriesterase homology protein; PhP; YhfV; member of a family of proteins related to phosphotriesterase (PTE); hydrolase 404835..405713 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155974 YP_003034626.1 CDS ECBD_0369 NC_012947.1 405724 406077 D KEGG: sfv:SFV_3383 hypothetical protein; hypothetical protein 405724..406077 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155975 YP_003034627.1 CDS ECBD_0370 NC_012947.1 406089 407393 D KEGG: elf:LF82_3287 uncharacterized protein YhfT; hypothetical protein 406089..407393 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155976 YP_003034628.1 CDS ECBD_0372 NC_012947.1 407934 408209 D PFAM: Insertion element protein; KEGG: hdu:HD1971 insertion element IS1 1/2/3/5/6 protein InsA; Insertion element protein 407934..408209 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155978 YP_003034629.1 CDS ECBD_0373 NC_012947.1 408128 408631 D PFAM: IS1 transposase; IS1 transposase 408128..408631 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155979 YP_003034630.1 CDS ECBD_0374 NC_012947.1 409420 410151 R may act as a transcriptional regulator of a fructoselysine-induced operon containing the yhfM, yhfN, yhfO, yhfP, yhfQ, and yhfR genes; DNA-binding transcriptional regulator FrlR complement(409420..410151) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155980 YP_003034631.1 CDS ECBD_0375 NC_012947.1 410251 411036 R PFAM: PfkB domain protein; KEGG: sbc:SbBS512_E3751 kinase, PfkB family; fructoselysine 6-kinase complement(410251..411036) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155981 YP_003034632.1 CDS ECBD_0376 NC_012947.1 411033 411863 R YhfOP; YhfP; YhfO; FrlC; catalyzes the interconversion of fructoselysine and psicoselysine; fructoselysine 3-epimerase complement(411033..411863) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155982 YP_003034633.1 CDS frlB NC_012947.1 411913 412935 R catalyzes the conversion of fructoselysine 6-phosphate to glucose 6-phosphate and lysine; fructoselysine-6-P-deglycase complement(411913..412935) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155983 YP_003034634.1 CDS frlA NC_012947.1 412956 414293 R inner membrane protein possibly involved in fructoselysine transport; member of the flr operon; fructoselysine transporter complement(412956..414293) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155984 YP_003034635.1 CDS ECBD_0379 NC_012947.1 414588 414755 R KEGG: sbc:SbBS512_E3747 lipoprotein; lipoprotein complement(414588..414755) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155985 YP_003034636.1 CDS cysG NC_012947.1 415002 416375 R multifunction enzyme consisting of uroporphyrin-III C-methyltransferase, precorrin-2 dehydrogenase and sirohydrochlorin ferrochelatase; catalyzes the methylation of uroporphyrinogen III to form precorrin-2, then catalyzes formation of sirohydrochlorin from precorrin-2 and finally catalyzed the formation of siroheme from sirohydrochlorin; siroheme synthase complement(415002..416375) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155986 YP_003034637.1 CDS ECBD_0381 NC_012947.1 416394 417200 R member of the FNT family of formate and nitrite transporters; nitrite transporter NirC complement(416394..417200) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155987 YP_003034638.1 CDS nirD NC_012947.1 417326 417652 R involved in reducing nitrite to ammonium to detoxify nitrite accumulation in anaerobic nitrate-respiring cells and regenerate NAD+; bounds to NirB, the cytoplasmic subunit, whose expression is induced at high nitrate concentrations; nitrite reductase small subunit complement(417326..417652) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155988 YP_003034639.1 CDS ECBD_0383 NC_012947.1 417649 420192 R TIGRFAM: nitrite reductase [NAD(P)H], large subunit; PFAM: nitrite and sulphite reductase 4Fe-4S region; FAD-dependent pyridine nucleotide-disulphide oxidoreductase; BFD domain protein [2Fe-2S]-binding domain protein; nitrite/sulfite reductase hemoprotein beta-component ferrodoxin domain protein; KEGG: sbc:SbBS512_E3741 nitrite reductase [NAD(P)H], large subunit; nitrite reductase (NAD(P)H), large subunit complement(417649..420192) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155989 YP_003034640.1 CDS ECBD_0384 NC_012947.1 420454 421635 R PFAM: major facilitator superfamily MFS_1; KEGG: sbo:SBO_3346 hypothetical protein; hypothetical protein complement(420454..421635) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155990 YP_003034641.1 CDS ECBD_0385 NC_012947.1 421906 422478 D PFAM: peptidyl-prolyl cis-trans isomerase cyclophilin type; KEGG: sbc:SbBS512_E3738 peptidyl-prolyl cis-trans isomerase A; peptidyl-prolyl cis-trans isomerase A (rotamase A) 421906..422478 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155991 YP_003034642.1 CDS ECBD_0386 NC_012947.1 422583 422750 D KEGG: sfv:SFV_3368 hypothetical protein; hypothetical protein 422583..422750 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155992 YP_003034643.1 CDS ECBD_0387 NC_012947.1 422740 423342 D PFAM: filamentation induced by cAMP protein Fic; KEGG: sfv:SFV_3367 cell filamentation protein Fic; cell filamentation protein Fic 422740..423342 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155993 YP_003034644.1 CDS ECBD_0388 NC_012947.1 423374 423937 D aminodeoxychorismate synthase subunit PabA; with PabB catalyzes the formation of 4-amino-4-deoxychorismate from chorismate and glutamine in para-aminobenzoate synthesis; PabA provides the glutamine amidotransferase activity; para-aminobenzoate synthase component II 423374..423937 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155994 YP_003034645.1 CDS argD NC_012947.1 424023 425243 D DapATase; bifunctional enzyme that functions in arginine and lysine biosynthetic pathways; catalyzes the formation of N-acetyl-L-glutamate 5-semialdehyde from 2-oxoglutarate and N(2)-acetyl-L-ornithine or N-succinyl-2-L-amino-6-oxoheptanedioate from 2-oxoglutarate and N-succinyl-L-2,6-diaminoheptanedioate; bifunctional N-succinyldiaminopimelate-aminotransferase/acetylornithine transaminase protein 424023..425243 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155995 YP_003034646.1 CDS ECBD_0390 NC_012947.1 425310 427400 R TIGRFAM: integral membrane protein, YccS/YhfK family; PFAM: protein of unknown function DUF893 YccS/YhfK; KEGG: sbc:SbBS512_E3734 integral membrane protein, YccS/YhfK family; integral membrane protein, YccS/YhfK family complement(425310..427400) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155996 YP_003034647.1 CDS ECBD_0391 NC_012947.1 427451 428083 R complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons; cAMP-regulatory protein complement(427451..428083) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155997 YP_003034648.1 CDS ECBD_0392 NC_012947.1 428385 428789 D PFAM: OsmC family protein; KEGG: sdy:SDY_3518 hypothetical protein; hypothetical protein 428385..428789 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155998 YP_003034649.1 CDS ECBD_0393 NC_012947.1 428844 429713 R PFAM: phosphoribulokinase/uridine kinase; KEGG: sfv:SFV_3361 phosphoribulokinase; phosphoribulokinase complement(428844..429713) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155999 YP_003034650.1 CDS ECBD_0394 NC_012947.1 429767 429985 R PFAM: protein of unknown function UPF0270; KEGG: sbc:SbBS512_E3729 hypothetical protein; hypothetical protein complement(429767..429985) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156000 YP_003034651.1 CDS ECBD_0395 NC_012947.1 429979 431001 R PFAM: alpha/beta hydrolase fold; KEGG: ssn:SSON_3484 predicted hydrolase; hydrolase complement(429979..431001) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156001 YP_003034652.1 CDS ECBD_0396 NC_012947.1 431001 432914 R PFAM: ABC transporter; SMART: ATPase AAA; KEGG: ssn:SSON_3483 ABC transporter ATP-binding protein; ABC transporter ATP-binding protein complement(431001..432914) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156002 YP_003034653.1 CDS ECBD_0397 NC_012947.1 433045 433596 D required for KefB activity; glutathione-regulated potassium-efflux system ancillary protein KefG 433045..433596 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156003 YP_003034654.1 CDS ECBD_0398 NC_012947.1 433596 435401 D involved in potassium efflux; glutathione-regulated potassium-efflux system protein KefB 433596..435401 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156004 YP_003034655.1 CDS ECBD_0399 NC_012947.1 435411 435611 D KEGG: sdy:SDY_3511 hypothetical protein; hypothetical protein 435411..435611 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156005 YP_003034656.1 CDS ECBD_0400 NC_012947.1 435706 436296 D rotamase; FKBP-type peptidyl-prolyl cis-trans isomerase 435706..436296 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156006 YP_003034657.1 CDS ECBD_0401 NC_012947.1 436345 436563 R PFAM: SlyX family protein; KEGG: sfv:SFV_3353 hypothetical protein; hypothetical protein complement(436345..436563) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156007 YP_003034658.1 CDS ECBD_0402 NC_012947.1 436784 437596 D rotamase; FKBP-type peptidyl-prolyl cis-trans isomerase 436784..437596 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156008 YP_003034659.1 CDS ECBD_0403 NC_012947.1 437763 438485 D PFAM: YheO domain protein; KEGG: sfx:S4398 hypothetical protein; YheO domain protein 437763..438485 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156009 YP_003034660.1 CDS ECBD_0404 NC_012947.1 438485 438871 D in Escherichai coli the heterohexameric TusBCD complex is involved in sulfur related that results in thiouridation to U34 position in some tRNAs; sulfur transfer complex subunit TusD 438485..438871 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156010 YP_003034661.1 CDS ECBD_0405 NC_012947.1 438871 439230 D in Escherichai coli the heterohexameric TusBCD complex is involved in sulfur related that results in thiouridation to U34 position in some tRNAs; sulfur relay protein TusC 438871..439230 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156011 YP_003034662.1 CDS ECBD_0406 NC_012947.1 439238 439525 D in Escherichai coli the heterohexameric TusBCD complex is involved in sulfur related that results in thiouridation to U34 position in some tRNAs; sulfur transfer complex subunit TusB 439238..439525 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156012 YP_003034663.1 CDS rpsL NC_012947.1 439651 440025 D interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone; located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side; mutations in the S12 gene confer streptomycin resistance; 30S ribosomal protein S12 439651..440025 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156013 YP_003034664.1 CDS ECBD_0408 NC_012947.1 440122 440592 D binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit; 30S ribosomal protein S7 440122..440592 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156014 YP_003034665.1 CDS ECBD_0409 NC_012947.1 440689 442803 D EF-G; promotes GTP-dependent translocation of the ribosome during translation; many organisms have multiple copies of this gene; elongation factor G 440689..442803 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156015 YP_003034666.1 CDS ECBD_0410 NC_012947.1 442874 444058 D EF-Tu; promotes GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis; when the tRNA anticodon matches the mRNA codon, GTP hydrolysis results; the inactive EF-Tu-GDP leaves the ribosome and release of GDP is promoted by elongation factor Ts; many prokaryotes have two copies of the gene encoding EF-Tu; elongation factor Tu 442874..444058 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159968 YP_003034667.1 CDS ECBD_0411 NC_012947.1 444107 444265 D hypothetical protein 444107..444265 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159401 YP_003034668.1 CDS ECBD_0412 NC_012947.1 444350 446410 D KEGG: elf:LF82_0302 bifunctional chitinase/lysozyme; PFAM: carbohydrate-binding family V/XII; SMART: carbohydrate-binding family V/XII; Lysozyme 444350..446410 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159402 YP_003034669.1 CDS ECBD_0413 NC_012947.1 446413 446529 D hypothetical protein 446413..446529 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159403 YP_003034670.1 CDS ECBD_0414 NC_012947.1 446579 446773 D PFAM: BFD domain protein [2Fe-2S]-binding domain protein; KEGG: elf:LF82_0223 bacterioferritin-associated ferredoxin; bacterioferritin-associated ferredoxin 446579..446773 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159404 YP_003034671.1 CDS ECBD_0415 NC_012947.1 446845 447321 D iron storage protein; bacterioferritin 446845..447321 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159405 YP_003034672.1 CDS ECBD_0416 NC_012947.1 447350 448027 R PFAM: peptidase A24A prepilin type IV; KEGG: kpn:KPN_00149 pullulanase Q protein; prepilin peptidase complement(447350..448027) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159406 YP_003034673.1 CDS ECBD_0417 NC_012947.1 448027 448488 R PFAM: general secretion pathway M protein; KEGG: tau:Tola_0358 general secretion pathway M protein; general secretion pathway protein M complement(448027..448488) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159407 YP_003034674.1 CDS ECBD_0418 NC_012947.1 448485 449648 R TIGRFAM: general secretion pathway protein L; PFAM: general secretion pathway L; KEGG: elf:LF82_0949 general secretion pathway protein L; general secretion pathway protein L complement(448485..449648) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159408 YP_003034675.1 CDS ECBD_0419 NC_012947.1 449663 450646 R PFAM: general secretion pathway protein K; KEGG: elf:LF82_0948 general secretion pathway protein K; general secretion pathway protein K complement(449663..450646) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159409 YP_003034676.1 CDS ECBD_0420 NC_012947.1 450639 451226 R TIGRFAM: general secretion pathway protein J; KEGG: elf:LF82_0947 general secretion pathway protein J; general secretion pathway protein J complement(450639..451226) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159410 YP_003034677.1 CDS ECBD_0421 NC_012947.1 451219 451596 R TIGRFAM: general secretion pathway protein I; PFAM: type II secretion system protein I/J; KEGG: elf:LF82_0946 general secretion pathway protein I; general secretion pathway protein I complement(451219..451596) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159411 YP_003034678.1 CDS ECBD_0422 NC_012947.1 451593 452102 R TIGRFAM: general secretion pathway protein H; KEGG: elf:LF82_0945 general secretion pathway protein H; general secretion pathway protein H complement(451593..452102) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159412 YP_003034679.1 CDS ECBD_0423 NC_012947.1 452110 452547 R TIGRFAM: general secretion pathway protein G; PFAM: type II secretion system protein G; KEGG: elf:LF82_0944 general secretion pathway protein G; general secretion pathway protein G complement(452110..452547) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159413 YP_003034680.1 CDS ECBD_0424 NC_012947.1 452557 453753 R TIGRFAM: general secretion pathway protein F; PFAM: type II secretion system protein; general secretion pathway protein F complement(452557..453753) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159414 YP_003034681.1 CDS ECBD_0425 NC_012947.1 453750 455231 R KEGG: elf:LF82_0942 general secretion pathway protein E; TIGRFAM: general secretory pathway protein E; PFAM: type II secretion system protein E; SMART: ATPase AAA; general secretory pathway protein E complement(453750..455231) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159415 YP_003034682.1 CDS ECBD_0426 NC_012947.1 455241 456257 R PFAM: type II and III secretion system protein; NolW domain protein; KEGG: elf:LF82_0941 general secretion pathway protein D; type II and III secretion system protein complement(455241..456257) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159416 YP_003034683.1 CDS ECBD_0427 NC_012947.1 456241 457056 R TIGRFAM: general secretion pathway protein C; KEGG: elf:LF82_0940 general secretion pathway protein C; general secretion pathway protein C complement(456241..457056) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159417 YP_003034684.1 CDS ECBD_0428 NC_012947.1 457236 458705 D PFAM: peptidoglycan-binding protein; KEGG: elf:LF82_0938 general secretion pathway protein A; peptidoglycan-binding protein 457236..458705 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159418 YP_003034685.1 CDS ECBD_0429 NC_012947.1 458707 459126 D calcium-binding protein that may be required for the initiation of chromosome replication; chromosome replication protein PioO 458707..459126 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159419 YP_003034686.1 CDS rpsJ NC_012947.1 459364 459675 D NusE; involved in assembly of the 30S subunit; in the ribosome, this protein is involved in the binding of tRNA; in Escherichia coli this protein was also found to be involved in transcription antitermination; NusB/S10 heterodimers bind boxA sequences in the leader RNA of rrn operons which is required for antitermination; binding of NusB/S10 to boxA nucleates assembly of the antitermination complex; 30S ribosomal protein S10 459364..459675 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159420 YP_003034687.1 CDS rplC NC_012947.1 459708 460337 D binds directly near the 3' end of the 23S rRNA, where it nucleates assembly of the 50S subunit; essential for peptidyltransferase activity; mutations in this gene confer resistance to tiamulin; 50S ribosomal protein L3 459708..460337 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159421 YP_003034688.1 CDS rplD NC_012947.1 460348 460953 D L4 is important during the early stages of 50S assembly; it initially binds near the 5' end of the 23S rRNA; 50S ribosomal protein L4 460348..460953 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159422 YP_003034689.1 CDS rplW NC_012947.1 460950 461252 D binds third domain of 23S rRNA and protein L29; part of exit tunnel; 50S ribosomal protein L23 460950..461252 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159423 YP_003034690.1 CDS rplB NC_012947.1 461270 462091 D one of the primary rRNA-binding proteins; required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation; 50S ribosomal protein L2 461270..462091 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159424 YP_003034691.1 CDS rpsS NC_012947.1 462108 462386 D protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA; 30S ribosomal protein S19 462108..462386 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159425 YP_003034692.1 CDS rplV NC_012947.1 462401 462733 D binds specifically to 23S rRNA during the early stages of 50S assembly; makes contact with all 6 domains of the 23S rRNA in the assembled 50S subunit and ribosome; mutations in this gene result in erythromycin resistance; located near peptidyl-transferase center; 50S ribosomal protein L22 462401..462733 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159426 YP_003034693.1 CDS rpsC NC_012947.1 462751 463452 D forms a complex with S10 and S14; binds the lower part of the 30S subunit head and the mRNA in the complete ribosome to position it for translation; 30S ribosomal protein S3 462751..463452 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159427 YP_003034694.1 CDS rplP NC_012947.1 463465 463875 D located in the peptidyl transferase center and may be involved in peptidyl transferase activity; similar to archaeal L10e; 50S ribosomal protein L16 463465..463875 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159428 YP_003034695.1 CDS ECBD_0439 NC_012947.1 463875 464066 D one of the stabilizing components for the large ribosomal subunit; 50S ribosomal protein L29 463875..464066 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159429 YP_003034696.1 CDS rpsQ NC_012947.1 464066 464320 D primary binding protein; helps mediate assembly; involved in translation fidelity; 30S ribosomal protein S17 464066..464320 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159430 YP_003034697.1 CDS rplN NC_012947.1 464485 464856 D binds to the 23S rRNA between the centers for peptidyl transferase and GTPase; 50S ribosomal protein L14 464485..464856 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159431 YP_003034698.1 CDS rplX NC_012947.1 464867 465181 D assembly initiator protein; binds to 5' end of 23S rRNA and nucleates assembly of the 50S; surrounds polypeptide exit tunnel; 50S ribosomal protein L24 464867..465181 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159432 YP_003034699.1 CDS rplE NC_012947.1 465196 465735 D part of 50S and 5S/L5/L18/L25 subcomplex; contacts 5S rRNA and P site tRNA; forms a bridge to the 30S subunit in the ribosome by binding to S13; 50S ribosomal protein L5 465196..465735 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159433 YP_003034700.1 CDS rpsN NC_012947.1 465750 466055 D located in the peptidyl transferase center and involved in assembly of 30S ribosome subunit; similar to what is observed with proteins L31 and L33, some proteins in this family contain CXXC motifs that are involved in zinc binding; if two copies are present in a genome, then the duplicated copy appears to have lost the zinc-binding motif and is instead regulated by zinc; the proteins in this group do not appear to have the zinc-binding motif; 30S ribosomal protein S14 465750..466055 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159434 YP_003034701.1 CDS rpsH NC_012947.1 466089 466481 D binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit; 30S ribosomal protein S8 466089..466481 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159435 YP_003034702.1 CDS rplF NC_012947.1 466494 467027 D ribosomal protein L6 appears to have arisen as a result of an ancient gene duplication as based on structural comparison of the Bacillus stearothermophilus protein; RNA-binding appears to be in the C-terminal domain; mutations in the L6 gene confer resistance to aminoglycoside antibiotics such as gentamicin and these occur in truncations of the C-terminal domain; it has been localized to a region between the base of the L7/L12 stalk and the central protuberance; 50S ribosomal protein L6 466494..467027 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159436 YP_003034703.1 CDS rplR NC_012947.1 467037 467390 D binds 5S rRNA along with protein L5 and L25; 50S ribosomal protein L18 467037..467390 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159437 YP_003034704.1 CDS rpsE NC_012947.1 467405 467908 D located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body; contacts S4 and S8; with S4 and S12 plays a role in translational accuracy; mutations in this gene result in spectinomycin resistance; 30S ribosomal protein S5 467405..467908 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159438 YP_003034705.1 CDS rpmD NC_012947.1 467912 468091 D L30 binds domain II of the 23S rRNA and the 5S rRNA; similar to eukaryotic protein L7; 50S ribosomal protein L30 467912..468091 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159439 YP_003034706.1 CDS rplO NC_012947.1 468095 468529 D late assembly protein; 50S ribosomal protein L15 468095..468529 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159440 YP_003034707.1 CDS secY NC_012947.1 468537 469868 D forms heterotrimeric complex in the membrane; in bacteria the complex consists of SecY which forms the channel pore and SecE and SecG; the SecG subunit is not essential; in bacteria translocation is driven via the SecA ATPase; preprotein translocase subunit SecY 468537..469868 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159441 YP_003034708.1 CDS rpmJ NC_012947.1 469900 470016 D smallest protein in the large subunit; similar to what is found with protein L31 and L33 several bacterial genomes contain paralogs which may be regulated by zinc; the protein from Thermus thermophilus has a zinc-binding motif and contains a bound zinc ion; the proteins in this group have the motif; 50S ribosomal protein L36 469900..470016 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159442 YP_003034709.1 CDS rpsM NC_012947.1 470163 470519 D located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA; makes contact with the large subunit via RNA-protein interactions and via protein-protein interactions with L5; contacts P-site tRNA; 30S ribosomal protein S13 470163..470519 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159443 YP_003034710.1 CDS ECBD_0454 NC_012947.1 470536 470925 D located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA; forms part of the Shine-Dalgarno cleft in the 70S ribosome; interacts with S7 and S18 and IF-3; 30S ribosomal protein S11 470536..470925 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159444 YP_003034711.1 CDS rpsD NC_012947.1 470959 471579 D primary rRNA binding protein; nucleates 30S assembly; involved in translational accuracy with proteins S5 and S12; interacts with protein S5; involved in autogeneously regulating ribosomal proteins by binding to pseudoknot structures in the polycistronic mRNA; interacts with transcription complex and functions similar to protein NusA in antitermination; 30S ribosomal protein S4 470959..471579 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159445 YP_003034712.1 CDS ECBD_0456 NC_012947.1 471605 472594 D catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Dimerization of the alpha subunit is the first step in the sequential assembly of subunits to form the holoenzyme; DNA-directed RNA polymerase subunit alpha 471605..472594 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159446 YP_003034713.1 CDS rplQ NC_012947.1 472635 473018 D is a component of the macrolide binding site in the peptidyl transferase center; 50S ribosomal protein L17 472635..473018 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159447 YP_003034714.1 CDS ECBD_0458 NC_012947.1 473125 473493 D KEGG: ssn:SSON_3434 hypothetical protein; hypothetical protein 473125..473493 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159448 YP_003034715.1 CDS zntR NC_012947.1 473504 473929 D mediates expression of the zinc export protein ZntA in response to high levels of zinc; member of MerR family of transcriptional regulators; zinc-responsive transcriptional regulator 473504..473929 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159449 YP_003034716.1 CDS ECBD_0460 NC_012947.1 473985 474203 D PFAM: protein of unknown function DUF331; KEGG: ssn:SSON_3432 hypothetical protein; hypothetical protein 473985..474203 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159450 YP_003034717.1 CDS mscL NC_012947.1 474200 474610 R forms homopentamer; channel that opens in response to pressure or hypoosmotic shock; large-conductance mechanosensitive channel complement(474200..474610) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159451 YP_003034718.1 CDS trkA NC_012947.1 474740 476116 R involved in potassium uptake; found to be peripherally associated with the inner membrane in Escherichia coli; contains an NAD-binding domain; potassium transporter peripheral membrane component complement(474740..476116) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159452 YP_003034719.1 CDS ECBD_0463 NC_012947.1 476138 477427 R catalyzes the methylation of cytosine at position 967 (m5C967) of 16S rRNA; SAM-dependent methyltransferase; 16S rRNA methyltransferase B complement(476138..477427) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159453 YP_003034720.1 CDS fmt NC_012947.1 477473 478420 R modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet) which is important in translation initiation; inactivation of this gene in Escherichia coli severely impairs growth; methionyl-tRNA formyltransferase complement(477473..478420) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159454 YP_003034721.1 CDS def NC_012947.1 478435 478944 R cleaves off formyl group from N-terminal methionine residues of newly synthesized proteins; binds iron(2+); peptide deformylase complement(478435..478944) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159455 YP_003034722.1 CDS ECBD_0466 NC_012947.1 479074 480198 D TIGRFAM: DNA protecting protein DprA; PFAM: SMF family protein; KEGG: elf:LF82_2142 protein smf; DNA protecting protein DprA 479074..480198 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159456 YP_003034723.1 CDS ECBD_0467 NC_012947.1 480170 480643 D PFAM: protein of unknown function DUF494; KEGG: sfv:SFV_3304 hypothetical protein; hypothetical protein 480170..480643 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159457 YP_003034724.1 CDS ECBD_0468 NC_012947.1 480672 481214 D PFAM: DNA topoisomerase type IA zn finger domain protein; DNA topoisomerase type IA zn finger domain protein 480672..481214 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159458 YP_003034725.1 CDS ECBD_0469 NC_012947.1 481219 481791 D RimN; YrdC; required for maturation of 16s RNA; binds preferentially double stranded RNA; ribosome maturation factor 481219..481791 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159459 YP_003034726.1 CDS aroE NC_012947.1 481796 482614 D AroE; catalyzes the conversion of shikimate to 3-dehydroshikimate; shikimate 5-dehydrogenase 481796..482614 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159460 YP_003034727.1 CDS ECBD_0471 NC_012947.1 482611 482868 D PFAM: protein of unknown function DUF1488; KEGG: sfv:SFV_3301 hypothetical protein; hypothetical protein 482611..482868 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159461 YP_003034728.1 CDS ECBD_0472 NC_012947.1 482844 483398 R KEGG: sfv:SFV_3300 transferase; transferase complement(482844..483398) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159462 YP_003034729.1 CDS ECBD_0473 NC_012947.1 489453 490211 R PFAM: ABC transporter; SMART: ATPase AAA; ABC transporter complement(489453..490211) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159470 YP_003034730.1 CDS ECBD_0474 NC_012947.1 490219 491322 R TIGRFAM: polar amino acid ABC transporter inner membrane subunit; PFAM: binding-protein-dependent transporters inner membrane component; KEGG: sdy:SDY_3447 transporter permease; polar amino acid ABC transporter permease complement(490219..491322) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159471 YP_003034731.1 CDS ECBD_0475 NC_012947.1 491332 492513 R TIGRFAM: polar amino acid ABC transporter inner membrane subunit; PFAM: binding-protein-dependent transporters inner membrane component; polar amino acid ABC transporter permease complement(491332..492513) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159472 YP_003034732.1 CDS ECBD_0476 NC_012947.1 492581 493606 R PFAM: extracellular solute-binding protein family 3; SMART: extracellular solute-binding protein family 3; KEGG: elf:LF82_3270 amino-acid ABC transporter-binding protein YhdW; extracellular solute-binding protein family 3 complement(492581..493606) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159473 YP_003034733.1 CDS ECBD_0477 NC_012947.1 493635 493811 R KEGG: sfv:SFV_3289 IS1 encoded protein; IS1 encoded protein complement(493635..493811) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159474 YP_003034734.1 CDS ECBD_0478 NC_012947.1 494037 494258 R hypothetical protein complement(494037..494258) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159475 YP_003034735.1 CDS ECBD_0479 NC_012947.1 494511 497615 R TIGRFAM: transporter hydrophobe/amphiphile efflux-1 (HAE1) family; PFAM: acriflavin resistance protein; KEGG: sbo:SBO_3260 integral transmembrane protein; transporter hydrophobe/amphiphile efflux-1 (HAE1) family complement(494511..497615) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159476 YP_003034736.1 CDS ECBD_0480 NC_012947.1 497627 498784 R TIGRFAM: efflux transporter RND family, MFP subunit; PFAM: secretion protein HlyD; KEGG: sbc:SbBS512_E3648 acriflavine resistance protein E; efflux transporter RND family, MFP subunit complement(497627..498784) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159477 YP_003034737.1 CDS ECBD_0481 NC_012947.1 499183 499845 D PFAM: Tetracycline transcriptional repressor MAATS-type domain protein; regulatory protein TetR; KEGG: sbc:SbBS512_E3647 transcriptional regulator EnvR; DNA-binding transcriptional regulator EnvR 499183..499845 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159478 YP_003034738.1 CDS ECBD_0482 NC_012947.1 499848 500027 R hypothetical protein complement(499848..500027) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159479 YP_003034739.1 CDS ECBD_0483 NC_012947.1 500111 500995 R PFAM: DNA methylase N-4/N-6 domain protein; KEGG: ssn:SSON_3403 methyltransferase; methyltransferase complement(500111..500995) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159480 YP_003034740.1 CDS fis NC_012947.1 501081 501377 R Stimulates excision of phage lambda; affects Mu development; acts as an activator of rRNA and iRNA transcription; DNA-binding protein Fis complement(501081..501377) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158996 YP_003034741.1 CDS ECBD_0485 NC_012947.1 501403 502368 R TIGRFAM: hypothetical protein; PFAM: dihydrouridine synthase; KEGG: sdy:SDY_3437 tRNA-dihydrouridine synthase B; tRNA-dihydrouridine synthase B complement(501403..502368) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158997 YP_003034742.1 CDS prmA NC_012947.1 502697 503578 R methylates ribosomal protein L11 at multiple amino acid positions; mutations of these genes in Escherichia coli or Thermus thermophilus has no apparent phenotype; ribosomal protein L11 methyltransferase complement(502697..503578) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158998 YP_003034743.1 CDS panF NC_012947.1 503590 505041 R mediates high affinitiy panthothenate transport which is stimulated by the presence of sodium ions; member of SSS family of sodium/solute symporters; the imported panthothenate is phosphorylated by panthothenate kinase; sodium/panthothenate symporter complement(503590..505041) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158999 YP_003034744.1 CDS ECBD_0488 NC_012947.1 505031 505273 R PFAM: protein of unknown function DUF997; KEGG: sdy:SDY_3434 hypothetical protein; hypothetical protein complement(505031..505273) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159000 YP_003034745.1 CDS ECBD_0489 NC_012947.1 505382 506731 R an AccC homodimer forms the biotin carboxylase subunit of the acetyl CoA carboxylase, an enzyme that catalyzes the formation of malonyl-CoA, which in turn controls the rate of fatty acid metabolism; acetyl-CoA carboxylase biotin carboxylase subunit complement(505382..506731) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159001 YP_003034746.1 CDS ECBD_0490 NC_012947.1 506742 507212 R composes the biotin carboxyl carrier protein subunit of the acetyl-CoA carboxylase complex, the enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, which in turn controls the rate of fatty acid metabolism; acetyl-CoA carboxylase biotin carboxyl carrier protein subunit complement(506742..507212) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159002 YP_003034747.1 CDS ECBD_0491 NC_012947.1 507354 507515 D hypothetical protein 507354..507515 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159003 YP_003034748.1 CDS ECBD_0493 NC_012947.1 508190 509164 R TIGRFAM: quinone oxidoreductase, YhdH/YhfP family; PFAM: alcohol dehydrogenase zinc-binding domain protein; alcohol dehydrogenase GroES domain protein; KEGG: ssn:SSON_3394 dehydrogenase; quinone oxidoreductase, YhdH/YhfP family complement(508190..509164) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159005 YP_003034749.1 CDS ECBD_0494 NC_012947.1 509316 511256 D regulates the degradation of the small RNAs CsrB and CsrC; may function to targate RNase E to specific RNA molecules; regulatory protein CsrD 509316..511256 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159006 YP_003034750.1 CDS ECBD_0495 NC_012947.1 511265 511453 R KEGG: sbc:SbBS512_E3283 hypothetical protein; hypothetical protein complement(511265..511453) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159007 YP_003034751.1 CDS ECBD_0496 NC_012947.1 511561 512604 D functions in MreBCD complex in some organisms; rod shape-determining protein MreB 511561..512604 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159008 YP_003034752.1 CDS ECBD_0497 NC_012947.1 512670 513773 D in some organisms this protein is a transmembrane protein while in others it is periplasmic; involved in some organisms with other components of the MreBCD complex and with penicillin binding proteins in the periplasm or cell wall; rod shape-determining protein MreC 512670..513773 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159009 YP_003034753.1 CDS ECBD_0498 NC_012947.1 513773 514261 D part of cell wall structural complex MreBCD; transmembrane component; rod shape-determining protein MreD 513773..514261 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159010 YP_003034754.1 CDS ECBD_0499 NC_012947.1 514270 514863 D Maf; overexpression in Bacillus subtilis inhibits septation in the dividing cell; Maf-like protein 514270..514863 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159011 YP_003034755.1 CDS ECBD_0500 NC_012947.1 514853 516322 D involved in the processing of the 5'end of 16S rRNA; ribonuclease G 514853..516322 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159012 YP_003034756.1 CDS ECBD_0501 NC_012947.1 516390 520190 D KEGG: elf:LF82_3266 uncharacterized protein YhdP; hypothetical protein 516390..520190 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159013 YP_003034757.1 CDS tldD NC_012947.1 520620 522065 D responsible for the proteolytic maturation of the E. coli pMccB17 plasmid-encoded microcin B17, an exported protein that targets the essential topoisomerase II DNA gyrase; degrades the E. coli plasmid F-encoded CcdA; protease TldD 520620..522065 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159014 YP_003034758.1 CDS ECBD_0503 NC_012947.1 522193 523122 R KEGG: sbo:SBO_3144 DNA-binding transcriptional regulator; DNA-binding transcriptional regulator complement(522193..523122) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159015 YP_003034759.1 CDS ECBD_0504 NC_012947.1 523305 523508 D membrane protein AaeX; the gene is a member of the aaeXAB operon; hypothetical protein 523305..523508 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159016 YP_003034760.1 CDS ECBD_0505 NC_012947.1 523516 524448 D with AaeB forms an efflux pump whose substrates are p-hydroxybenzoic acid, 6-hydroxy-2-naphthoic and 2-hydroxycinnamate; p-hydroxybenzoic acid efflux subunit AaeA 523516..524448 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159017 YP_003034761.1 CDS ECBD_0506 NC_012947.1 524454 526421 D with AaeA forms an efflux pump whose substrates are p-hydroxybenzoic acid 6-hydroxy-2-naphthoic and 2-hydroxycinnamate; p-hydroxybenzoic acid efflux subunit AaeB 524454..526421 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159018 YP_003034762.1 CDS ECBD_0507 NC_012947.1 526513 526785 D KEGG: sdy:SDY_3415 hypothetical protein; hypothetical protein 526513..526785 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159019 YP_003034763.1 CDS ECBD_0508 NC_012947.1 526841 527104 R KEGG: sbc:SbBS512_E3538 protein YcfR; protein YcfR complement(526841..527104) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159020 YP_003034764.1 CDS ECBD_0509 NC_012947.1 527469 527939 R regulates arginine biosynthesis when complexed with arginine by binding at site that overlap the promotors of the arginine biosynthesis genes; arginine repressor complement(527469..527939) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159021 YP_003034765.1 CDS ECBD_0510 NC_012947.1 527923 528087 R KEGG: spq:SPAB_04187 hypothetical protein; hypothetical protein complement(527923..528087) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159022 YP_003034766.1 CDS ECBD_0511 NC_012947.1 528374 529312 D oxidizes malate to oxaloacetate; malate dehydrogenase 528374..529312 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159023 YP_003034767.1 CDS ECBD_0512 NC_012947.1 529374 530441 R KEGG: sfv:SFV_3262 serine endoprotease; TIGRFAM: periplasmic serine protease DegS; PFAM: peptidase S1 and S6 chymotrypsin/Hap; PDZ/DHR/GLGF domain protein; SMART: PDZ/DHR/GLGF domain protein; serine endoprotease complement(529374..530441) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159024 YP_003034768.1 CDS ECBD_0513 NC_012947.1 530531 531898 R KEGG: sbc:SbBS512_E3542 serine peptidase DegQ; TIGRFAM: protease Do; PFAM: PDZ/DHR/GLGF domain protein; peptidase S1 and S6 chymotrypsin/Hap; SMART: PDZ/DHR/GLGF domain protein; serine endoprotease complement(530531..531898) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159025 YP_003034769.1 CDS ECBD_0514 NC_012947.1 532052 532450 R PFAM: protein of unknown function DUF1043; KEGG: elf:LF82_3254 cytochrome d ubiquinol oxidase subunit III; cytochrome d ubiquinol oxidase subunit III complement(532052..532450) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159026 YP_003034770.1 CDS ECBD_0515 NC_012947.1 532644 533771 D PFAM: AFG1-family ATPase; KEGG: sfv:SFV_3259 hypothetical protein; AFG1-family ATPase 532644..533771 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159027 YP_003034771.1 CDS rplM NC_012947.1 533990 534418 D in Escherichia coli this protein is one of the earliest assembly proteins in the large subunit; 50S ribosomal protein L13 533990..534418 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159028 YP_003034772.1 CDS rpsI NC_012947.1 534434 534826 D forms a direct contact with the tRNA during translation; 30S ribosomal protein S9 534434..534826 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159029 YP_003034773.1 CDS sspA NC_012947.1 535221 535859 D transcriptional activator; required for activation of bacteriophage P1 late promoter; induced by starvation; stringent starvation protein A 535221..535859 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159030 YP_003034774.1 CDS ECBD_0519 NC_012947.1 535865 536362 D PFAM: stringent starvation protein B; KEGG: sfx:S3483 ClpXP protease specificity-enhancing factor; ClpXP protease specificity-enhancing factor 535865..536362 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159031 YP_003034775.1 CDS ECBD_0520 NC_012947.1 536405 537772 R YhcL; uncharacterized member of the DcuC family of anaerobic dicarboxylate transporters; probable role in transport of carboxylates across the inner membrane from the periplasm; its encoding gene is not essential for growth and is probably cryptic; cryptic C4-dicarboxylate transporter DcuD complement(536405..537772) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159032 YP_003034776.1 CDS ECBD_0521 NC_012947.1 538152 538943 D transcriptional repressor of the nan operon that encodes proteins involved in sialic acid utilization; transcriptional regulator NanR 538152..538943 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159033 YP_003034777.1 CDS ECBD_0522 NC_012947.1 539065 539958 D catalyzes the formation of pyruvate and N-acetylmannosamine from N-acetylneuraminic acid; N-acetylneuraminate lyase 539065..539958 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159034 YP_003034778.1 CDS ECBD_0523 NC_012947.1 540067 541557 D TIGRFAM: sialic acid transporter; PFAM: major facilitator superfamily MFS_1; general substrate transporter; KEGG: sbc:SbBS512_E3552 MFS transporter sialate:H+ symporter (SHS) family; sialic acid transporter 540067..541557 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159035 YP_003034779.1 CDS ECBD_0524 NC_012947.1 541605 542294 D Converts N-acetylmannosamine-6-phosphate to N-acetylglucosamine-6-phosphate; N-acetylmannosamine-6-phosphate 2-epimerase 541605..542294 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159036 YP_003034780.1 CDS ECBD_0525 NC_012947.1 542291 543166 D catalyzes the phosphorylation of the N-acetylmannosamine (ManNAc) liberated from N-acetyl-neuraminic acid by the nanA protein; N-acetylmannosamine kinase 542291..543166 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159037 YP_003034781.1 CDS ECBD_0526 NC_012947.1 543163 543627 D PFAM: conserved hypothetical protein; KEGG: sfv:SFV_3246 hypothetical protein; hypothetical protein 543163..543627 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159038 YP_003034782.1 CDS ECBD_0527 NC_012947.1 543703 544008 R KEGG: sfx:S3473 IS600 orf; IS600 orf complement(543703..544008) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159039 YP_003034783.1 CDS ECBD_0528 NC_012947.1 544025 544504 R PFAM: protein of unknown function DUF1016; KEGG: elf:LF82_3256 uncharacterized protein YhcG; hypothetical protein complement(544025..544504) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159040 YP_003034784.1 CDS gltD NC_012947.1 544688 546106 R glutamate synthase is composed of subunits alpha and beta; subunit beta is a flavin adenine dinucleotide-NADPH dependent oxidoreductase; provides electrons to the subunit alpha, which binds L-glutamine and 2-oxoglutarate and forms L-glutamate; glutamate synthase subunit beta complement(544688..546106) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159041 YP_003034785.1 CDS gltB NC_012947.1 546119 550672 R catalyzes the formation of glutamate from glutamine and alpha-ketoglutarate; glutamate synthase subunit alpha complement(546119..550672) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159042 YP_003034786.1 CDS ECBD_0531 NC_012947.1 551254 552183 D KEGG: sbo:SBO_3171 hypothetical protein; TIGRFAM: conserved hypothetical protein; PFAM: radical SAM protein; SMART: Elongator protein 3/MiaB/NifB; hypothetical protein 551254..552183 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159043 YP_003034787.1 CDS ECBD_0532 NC_012947.1 552279 554615 D sensor-regulator protein which regulates the expression of many genes in response to respiratory growth conditions including anaerobic repression of the arc modulon; hybrid sensory histidine kinase in two-component regulatory system with ArcA; aerobic respiration control sensor protein ArcB 552279..554615 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159044 YP_003034788.1 CDS ECBD_0533 NC_012947.1 554845 555498 D PFAM: ThiJ/PfpI domain protein; KEGG: sfv:SFV_3239 isoprenoid biosynthesis protein with amidotransferase-like domain; isoprenoid biosynthesis protein with amidotransferase-like domain 554845..555498 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159045 YP_003034789.1 CDS mtgA NC_012947.1 555495 556223 D glycosyltransferase; polymerizes glycan strands in the peptidoglycan; monofunctional biosynthetic peptidoglycan transglycosylase 555495..556223 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159046 YP_003034790.1 CDS ECBD_0535 NC_012947.1 556220 556852 R KEGG: sfx:S3465 hypothetical protein; hypothetical protein complement(556220..556852) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159047 YP_003034791.1 CDS ECBD_0536 NC_012947.1 557066 557338 R TIGRFAM: phosphocarrier, HPr family; PFAM: phosphoryl transfer system HPr; KEGG: sfv:SFV_3236 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr); phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) complement(557066..557338) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159048 YP_003034792.1 CDS ECBD_0537 NC_012947.1 557335 558189 R PFAM: conserved hypothetical protein; KEGG: sbc:SbBS512_E3565 hypothetical protein; hypothetical protein complement(557335..558189) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159049 YP_003034793.1 CDS ECBD_0538 NC_012947.1 558235 558726 R TIGRFAM: PTS IIA-like nitrogen-regulatory protein PtsN; PFAM: phosphoenolpyruvate-dependent sugar phosphotransferase system EIIA 2; KEGG: sfv:SFV_3234 PTS IIA-like nitrogen-regulatory protein PtsN; PTS system transporter subunit IIA-like nitrogen-regulatory protein PtsN complement(558235..558726) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159050 YP_003034794.1 CDS ECBD_0539 NC_012947.1 558844 559131 R YhbH; resting ribosome-binding protein involved in ribosome stabilization and preservation in stationary phase; binds specifically 100S ribosomes (an inactive ribosome product of a 70S ribosome dimerization); seems to be involved in modulation of the sigma(54) (RpoN) activity for quorum sensing; sigma(54) modulation protein complement(558844..559131) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159051 YP_003034795.1 CDS ECBD_0540 NC_012947.1 559154 560587 R sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; sigma 54 factor is responsible for the expression of enzymes involved in nitrogen assimilation and metabolism; the rhizobia often have 2 copies of this sigma factor; in Rhizobium etli RpoN1 shown to be involved in the assimilation of several nitrogen and carbon sources during free-living aerobic growth and RpoN2 is involved in symbiotic nitrogen fixation; in Bradyrhizobium both RpoN1 and N2 are functional in free-living and symbiotic conditions, rpoN1 gene was regulated in response to oxygen; RNA polymerase factor sigma-54 complement(559154..560587) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159052 YP_003034796.1 CDS ECBD_0541 NC_012947.1 560635 561360 R PFAM: ABC transporter; SMART: ATPase AAA; KEGG: sdy:SDY_3382 ABC transporter ATP-binding protein YhbG; ABC transporter ATP-binding protein complement(560635..561360) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159053 YP_003034797.1 CDS ECBD_0542 NC_012947.1 561367 561924 R LptA; periplasmic binding protein part of a ABC superfamily of uptake transporters; interacts with LptB protein, the ATP binding component of the uptake system; lipopolysaccharide transport periplasmic protein LptA complement(561367..561924) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159054 YP_003034798.1 CDS ECBD_0543 NC_012947.1 561893 562468 R PFAM: protein of unknown function DUF1239; KEGG: sfv:SFV_3229 hypothetical protein; hypothetical protein complement(561893..562468) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159055 YP_003034799.1 CDS ECBD_0544 NC_012947.1 562465 563031 R forms homotetramers; catalyzes hydrolysis of KDO 8-P to KDO and inorganic phosphate; functions in lipopolysaccharide biosynthesis; 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase complement(562465..563031) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159056 YP_003034800.1 CDS ECBD_0545 NC_012947.1 563052 564038 R KEGG: sbc:SbBS512_E3573 arabinose 5-phosphate isomerase; TIGRFAM: KpsF/GutQ family protein; PFAM: sugar isomerase (SIS); CBS domain containing protein; D-arabinose 5-phosphate isomerase complement(563052..564038) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159057 YP_003034801.1 CDS ECBD_0546 NC_012947.1 564052 565029 R YrbG; inner membrane protein involved in cell envelope integrity; sodium ion/calcium ion exchanger; in E. coli it is non essential for cell viability; member of the YRBG family of cation/Ca2+ exchangers; calcium/sodium:proton antiporter complement(564052..565029) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159058 YP_003034802.1 CDS ECBD_0547 NC_012947.1 565239 566048 D ATP-binding subunit of a ABC toluene efflux transporter; ABC transporter ATP-binding protein 565239..566048 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159059 YP_003034803.1 CDS ECBD_0548 NC_012947.1 566056 566838 D PFAM: protein of unknown function DUF140; KEGG: sbc:SbBS512_E3576 toluene ABC transporter permease; hypothetical protein 566056..566838 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159060 YP_003034804.1 CDS ECBD_0549 NC_012947.1 566843 567394 D PFAM: hypothetical protein; KEGG: sbc:SbBS512_E3577 Mce family protein; hypothetical protein 566843..567394 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159061 YP_003034805.1 CDS ECBD_0550 NC_012947.1 567413 568048 D PFAM: toluene tolerance family protein; KEGG: sfv:SFV_3222 hypothetical protein; toluene tolerance family protein 567413..568048 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159062 YP_003034806.1 CDS ECBD_0551 NC_012947.1 568048 568341 D KEGG: sfv:SFV_3221 hypothetical protein; hypothetical protein 568048..568341 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159063 YP_003034807.1 CDS ECBD_0552 NC_012947.1 568486 568755 D PFAM: BolA family protein; KEGG: sfv:SFV_3220 hypothetical protein; BolA family protein 568486..568755 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159064 YP_003034808.1 CDS ECBD_0553 NC_012947.1 568810 570069 D adds enolpyruvyl to UDP-N-acetylglucosamine as a component of cell wall formation; gram-positive bacteria have 2 copies of MurA which are active; UDP-N-acetylglucosamine 1-carboxyvinyltransferase 568810..570069 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159065 YP_003034809.1 CDS ECBD_0554 NC_012947.1 570117 570395 R activator of maltose metabolism genes; DNA-binding transcriptional regulator Nlp complement(570117..570395) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159066 YP_003034810.1 CDS ECBD_0555 NC_012947.1 570624 571595 R PFAM: polyprenyl synthetase; KEGG: sbo:SBO_3195 octaprenyl diphosphate synthase; octaprenyl diphosphate synthase complement(570624..571595) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159067 YP_003034811.1 CDS rplU NC_012947.1 571854 572165 D TIGRFAM: ribosomal protein L21; PFAM: ribosomal protein L21; KEGG: sdy:SDY_3367 50S ribosomal protein L21; 50S ribosomal protein L21 571854..572165 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159068 YP_003034812.1 CDS rpmA NC_012947.1 572186 572443 D involved in the peptidyltransferase reaction during translation; 50S ribosomal protein L27 572186..572443 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159069 YP_003034813.1 CDS ECBD_0558 NC_012947.1 572570 573535 D PFAM: protein of unknown function DUF6 transmembrane; KEGG: sbo:SBO_3198 hypothetical protein; hypothetical protein 572570..573535 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159070 YP_003034814.1 CDS obgE NC_012947.1 573551 574723 D essential GTPase; exhibits high exchange rate for GTP/GDP; associates with 50S ribosomal subunit; involved in regulation of chromosomal replication; GTPase ObgE 573551..574723 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159071 YP_003034815.1 CDS ECBD_0560 NC_012947.1 574909 576342 R penicillin binding protein 4; penicillin sensitive; catalyzes the formation of D-alanine from D-alanyl-D-alanine; one of four, DD-carboxypeptidase low-molecular weight penicillin-binding proteins that remove terminal D-alanine from pentapeptide side chains; D-alanyl-D-alanine carboxypeptidase/endopeptidase complement(574909..576342) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159072 YP_003034816.1 CDS greA NC_012947.1 576590 577066 D necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites; arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus; transcription elongation factor GreA 576590..577066 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159073 YP_003034817.1 CDS ECBD_0562 NC_012947.1 577222 577515 R RNA binding protein found associated to pre-50S subunit of the ribosome; role in ribosome assembly; necessary for optimal growth but not cell viability; RNA-binding protein YhbY complement(577222..577515) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159074 YP_003034818.1 CDS rrmJ NC_012947.1 577641 578270 D Specifically methylates the uridine in position 2552 of 23S rRNA in the fully assembled 50S ribosomal subunit; 23S rRNA methyltransferase J 577641..578270 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159075 YP_003034819.1 CDS hflB NC_012947.1 578361 580304 D inner membrane metalloprotease; may be involved in degradation of aberrant cytoplasmic and membrane proteins; ATP-dependent metalloprotease 578361..580304 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159076 YP_003034820.1 CDS folP NC_012947.1 580394 581242 D catalyzes the formation of dihydropteroate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate; dihydropteroate synthase 580394..581242 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159725 YP_003034821.1 CDS glmM NC_012947.1 581235 582572 D catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate; phosphoglucosamine mutase 581235..582572 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158745 YP_003034822.1 CDS secG NC_012947.1 582800 583132 D TIGRFAM: preprotein translocase, SecG subunit; PFAM: Preprotein translocase SecG subunit; KEGG: efe:EFER_3152 preprotein translocase membrane subunit; preprotein translocase subunit SecG 582800..583132 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158746 YP_003034823.1 CDS ECBD_0568 NC_012947.1 583692 585317 D PFAM: sulfatase; KEGG: elf:LF82_3252 UPF0141 inner membrane protein YhbX; sulfatase 583692..585317 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158748 YP_003034824.1 CDS ECBD_0569 NC_012947.1 585325 586668 R catalyzes the formation of arginosuccinate from citrulline and aspartate in arginine biosynthesis; argininosuccinate synthase complement(585325..586668) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158749 YP_003034825.1 CDS ECBD_0570 NC_012947.1 587329 587751 D in Streptococcus pneumoniae this gene was found to be essential; structure determination of the Streptococcus protein shows that it is similar to a number of other proteins; hypothetical protein 587329..587751 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158751 YP_003034826.1 CDS nusA NC_012947.1 587779 589266 D modifies transcription through interactions with RNA polymerase affecting elongation, readthrough, termination, and antitermination; transcription elongation factor NusA 587779..589266 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158752 YP_003034827.1 CDS infB NC_012947.1 589291 591963 D Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits during initiation of protein synthesis. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex; translation initiation factor IF-2 589291..591963 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158753 YP_003034828.1 CDS rbfA NC_012947.1 592127 592528 D associates with free 30S ribosomal subunits; essential for efficient processing of 16S rRNA; in Escherichia coli rbfA is induced by cold shock; ribosome-binding factor A 592127..592528 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158754 YP_003034829.1 CDS truB NC_012947.1 592528 593472 D catalyzes isomerization of specific uridines in RNA to pseudouridine; responsible for residues in T loops of many tRNAs; tRNA pseudouridine synthase B 592528..593472 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158755 YP_003034830.1 CDS rpsO NC_012947.1 593621 593890 D primary rRNA binding protein; helps nucleate assembly of 30S; binds directly to the 16S rRNA and an intersubunit bridge to the 23S rRNA; autoregulates translation through interactions with the mRNA leader sequence; 30S ribosomal protein S15 593621..593890 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158756 YP_003034831.1 CDS ECBD_0576 NC_012947.1 594137 596272 D KEGG: ssn:SSON_3310 polynucleotide phosphorylase/polyadenylase; PFAM: 3' exoribonuclease; polynucleotide phosphorylase, phosphorolytic RNA-binding, bacterial/organelle-type; RNA binding S1 domain protein; Exoribonuclease, phosphorolytic domain 2; KH type 1 domain protein; SMART: RNA binding S1 domain protein; KH domain protein; polynucleotide phosphorylase/polyadenylase 594137..596272 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158757 YP_003034832.1 CDS ECBD_0577 NC_012947.1 596381 597265 D lipoprotein that appears to be involved in cell division; interacts with the periplasmic protease Prc and may be activated by protease processing; lipoprotein NlpI 596381..597265 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158758 YP_003034833.1 CDS ECBD_0578 NC_012947.1 597445 599334 D participates in the assembly of the large subunit of the ribosome; plays a key role in optimal cell growth at low temperature and is required for normal cell division; ATP-dependent RNA helicase DeaD 597445..599334 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158759 YP_003034834.1 CDS ECBD_0579 NC_012947.1 599488 600732 D tryptophan transporter of high affinity; tryptophan permease 599488..600732 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158760 YP_003034835.1 CDS ECBD_0580 NC_012947.1 600850 601857 R PFAM: Luciferase-like monooxygenase; KEGG: sbo:SBO_3222 hypothetical protein; hypothetical protein complement(600850..601857) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158761 YP_003034836.1 CDS ECBD_0581 NC_012947.1 601938 602816 R PFAM: peptidase U32; KEGG: sdy:SDY_3338 hypothetical protein; peptidase U32 complement(601938..602816) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158762 YP_003034837.1 CDS ECBD_0582 NC_012947.1 602825 603820 R PFAM: peptidase U32; KEGG: sfv:SFV_3188 collagenase; peptidase U32 complement(602825..603820) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158763 YP_003034838.1 CDS ECBD_0583 NC_012947.1 604029 604553 D PFAM: sterol-binding domain protein; KEGG: efe:EFER_3120 lipid carrier protein; sterol-binding domain protein 604029..604553 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158764 YP_003034839.1 CDS ECBD_0584 NC_012947.1 604547 605050 D PFAM: GCN5-related N-acetyltransferase; KEGG: sdy:SDY_3335 hypothetical protein; GCN5-related N-acetyltransferase 604547..605050 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158765 YP_003034840.1 CDS ECBD_0585 NC_012947.1 605037 605348 R PFAM: excinuclease ABC C subunit domain protein; SMART: excinuclease ABC C subunit domain protein; KEGG: sfv:SFV_3185 GIY-YIG nuclease superfamily protein; GIY-YIG nuclease superfamily protein complement(605037..605348) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158766 YP_003034841.1 CDS ECBD_0586 NC_012947.1 605390 605833 D KEGG: sfv:SFV_3184 hypothetical protein; hypothetical protein 605390..605833 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158767 YP_003034842.1 CDS ECBD_0587 NC_012947.1 605813 606331 R TIGRFAM: intracellular protease PfpI family; PFAM: ThiJ/PfpI domain protein; KEGG: sdy:SDY_3332 hypothetical protein; intracellular protease PfpI family complement(605813..606331) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158768 YP_003034843.1 CDS ECBD_0588 NC_012947.1 606459 607094 D PFAM: NAD-dependent epimerase/dehydratase; KEGG: elf:LF82_3683 uncharacterized protein YraR; NAD-dependent epimerase/dehydratase 606459..607094 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158769 YP_003034844.1 CDS ECBD_0589 NC_012947.1 607167 608207 D PFAM: permease; KEGG: ssn:SSON_3297 hypothetical protein; permease 607167..608207 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158770 YP_003034845.1 CDS ECBD_0590 NC_012947.1 608321 608896 R PFAM: transport-associated; SMART: transport-associated and nodulation region; KEGG: elf:LF82_3681 uncharacterized protein YraP; hypothetical protein complement(608321..608896) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158771 YP_003034846.1 CDS ECBD_0591 NC_012947.1 608906 609496 R Required for the timely initiation of chromosomal replication via direct interactions with the dnaA initiator protein; DnaA initiator-associating protein DiaA complement(608906..609496) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158772 YP_003034847.1 CDS ECBD_0592 NC_012947.1 609516 609911 R PFAM: protein of unknown function UPF0102; KEGG: ssn:SSON_3294 hypothetical protein; hypothetical protein complement(609516..609911) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158773 YP_003034848.1 CDS ECBD_0593 NC_012947.1 609869 611905 R PFAM: LppC family lipoprotein; KEGG: ssn:SSON_3293 glycosylase; LppC family lipoprotein complement(609869..611905) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158774 YP_003034849.1 CDS ECBD_0594 NC_012947.1 611970 612830 D PFAM: uroporphyrin-III C/tetrapyrrole (Corrin/Porphyrin) methyltransferase; KEGG: sdy:SDY_3325 hypothetical protein; uroporphyrin-III C/tetrapyrrole methyltransferase 611970..612830 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158775 YP_003034850.1 CDS ECBD_0595 NC_012947.1 612873 613964 R KEGG: sfv:SFV_3175 fimbrial protein; fimbrial protein complement(612873..613964) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158776 YP_003034851.1 CDS ECBD_0596 NC_012947.1 613975 616491 R PFAM: fimbrial biogenesis outer membrane usher protein; fimbrial biogenesis outer membrane usher protein complement(613975..616491) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158777 YP_003034852.1 CDS ECBD_0597 NC_012947.1 616521 617216 R PFAM: Pili assembly chaperone, N-terminal; Pili assembly chaperone, C-terminal; KEGG: sfv:SFV_3171 chaperone; Pili assembly chaperone, N-terminal complement(616521..617216) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158778 YP_003034853.1 CDS ECBD_0598 NC_012947.1 617296 617880 R PFAM: fimbrial protein; fimbrial protein complement(617296..617880) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158779 YP_003034854.1 CDS ECBD_0599 NC_012947.1 618281 618982 R PFAM: glucosamine/galactosamine-6-phosphate isomerase; KEGG: sfx:S3393 galactosamine-6-phosphate isomerase; galactosamine-6-phosphate isomerase complement(618281..618982) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158780 YP_003034855.1 CDS ECBD_0600 NC_012947.1 619037 619828 R catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane; involved in N-acetylgalactosamine transport; PTS system N-acetylgalactosamine-specific transporter subunit IID complement(619037..619828) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158781 YP_003034856.1 CDS ECBD_0601 NC_012947.1 619818 620621 R catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane;protein IIA transfers a phosphoryl group to IIB which then transfers the phosphoryl group to the sugar; IIC forms the translocation channel for the sugar uptake; involved in N-acetylgalactosamine transport; PTS system N-acetylgalactosamine-specific transporter subunit IIC complement(619818..620621) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158782 YP_003034857.1 CDS ECBD_0602 NC_012947.1 620660 621136 R catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane; protein IIA transfers a phosphoryl group to IIB which then transfers the phosphoryl group to the sugar; IIC forms the translocation channel for the sugar uptake; involved in N-acetylgalactosamine transport; PTS system N-acetylgalactosamine-specific transporter subunit IIB complement(620660..621136) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158783 YP_003034858.1 CDS kbaY NC_012947.1 621303 622163 R catalyzes the reversible reaction of dihydroxyacetone phosphate with glyceraldehyde 3-phosphate to produce tagatose 1,6-bisphosphate; in enteric bacteria there are two D-tagatose 1,6-bisphosphate-specific aldolases: KbaY (also called AgaY), involved in catabolism of N-acetyl-galactosamine and D-galactosamine, and GatY which is part of the galactitol catabolism pathway; tagatose-bisphosphate aldolase complement(621303..622163) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158784 YP_003034859.1 CDS ECBD_0604 NC_012947.1 622176 623330 R TIGRFAM: sugar isomerase, AgaS family; PFAM: sugar isomerase (SIS); sugar isomerase, AgaS family complement(622176..623330) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158785 YP_003034860.1 CDS ECBD_0605 NC_012947.1 623681 624814 R KEGG: elf:LF82_p515 N-acetylgalactosamine-6-phosphate deacetylase; TIGRFAM: N-acetylglucosamine-6-phosphate deacetylase; PFAM: amidohydrolase; N-acetylglucosamine-6-phosphate deacetylase complement(623681..624814) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158786 YP_003034861.1 CDS ECBD_0606 NC_012947.1 624811 625245 R PFAM: PTS system fructose subfamily IIA component; KEGG: elf:LF82_p514 phosphotransferase system enzyme subunit IIA component); PTS system fructose subfamily IIA component complement(624811..625245) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158787 YP_003034862.1 CDS ECBD_0607 NC_012947.1 625263 626141 R PFAM: PTS system mannose/fructose/sorbose family IID component; KEGG: efe:EFER_4358 hexose/hexosamine family PTS permease IID component; PTS system mannose/fructose/sorbose family IID component complement(625263..626141) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158788 YP_003034863.1 CDS ECBD_0608 NC_012947.1 626131 626910 R PFAM: phosphotransferase system PTS sorbose-specific IIC subunit; phosphotransferase system PTS sorbose-specific IIC subunit complement(626131..626910) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158789 YP_003034864.1 CDS ECBD_0609 NC_012947.1 626921 627394 R catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane; protein IIA transfers a phosphoryl group to IIB which then transfers the phosphoryl group to the sugar; IIC forms the translocation channel for the sugar uptake;involved in N-acetylgalactosamine transport; PTS system N-acetylgalactosamine-specific transporter subunit IIB complement(626921..627394) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158790 YP_003034865.1 CDS ECBD_0610 NC_012947.1 627417 628697 R TIGRFAM: D-tagatose-bisphosphate aldolase, class II, non-catalytic subunit; PFAM: D-tagatose-bisphosphate aldolase class II accessory protein AgaZ; KEGG: sfx:S3382 tagatose 6-phosphate kinase 2; D-tagatose-bisphosphate aldolase, class II, non-catalytic subunit complement(627417..628697) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158791 YP_003034866.1 CDS ECBD_0611 NC_012947.1 628946 629755 D transcriptional repressor for the agaZVWA and agaSYBCDI operons; DNA-binding transcriptional regulator AgaR 628946..629755 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158792 YP_003034867.1 CDS ECBD_0613 NC_012947.1 630274 630609 R SohA; PrlF; involved in protein secretion; when overproduced or mutated, it induces growth defect and increased export of a reporter protein; a PrlF mutation induces the activity of the Lon protease and a Lon-deficient strain suppresses the phenotype conferred by the PrlF mutation; regulator PrlF complement(630274..630609) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158794 YP_003034868.1 CDS ECBD_0614 NC_012947.1 630757 632328 R TIGRFAM: galactarate dehydratase; PFAM: D-galactarate dehydratase/altronate hydrolase domain protein; SAF domain protein; galactarate dehydratase complement(630757..632328) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158795 YP_003034869.1 CDS ECBD_0615 NC_012947.1 632703 634037 D TIGRFAM: d-galactonate transporter; PFAM: major facilitator superfamily MFS_1; d-galactonate transporter 632703..634037 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158796 YP_003034870.1 CDS ECBD_0616 NC_012947.1 634053 634823 D cleaves 5-dehydro-4-deoxy-glucarate and 2-dehydro-3-deoxy-D-glucarate; alpha-dehydro-beta-deoxy-D-glucarate aldolase 634053..634823 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158797 YP_003034871.1 CDS garR NC_012947.1 634853 635743 D catalyzes the reduction of tartronate semialdehyde to glycerate; tartronate semialdehyde reductase 634853..635743 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158798 YP_003034872.1 CDS ECBD_0618 NC_012947.1 635840 636985 D KEGG: sbo:SBO_2989 glycerate kinase I; TIGRFAM: glycerate kinase; PFAM: glycerate kinase; glycerate kinase I 635840..636985 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158799 YP_003034873.1 CDS ECBD_0619 NC_012947.1 637987 639090 R KEGG: sfv:SFV_3162 hypothetical protein; hypothetical protein complement(637987..639090) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158801 YP_003034874.1 CDS ECBD_0620 NC_012947.1 639112 639651 R hypothetical protein complement(639112..639651) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158802 YP_003034875.1 CDS ECBD_0621 NC_012947.1 639907 640263 R participates in controlling the expression of several structural genes for the enzyme threonine dehydratase; TdcR activates the tdcABCDEFG operon; DNA-binding transcriptional activator TdcR complement(639907..640263) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158803 YP_003034876.1 CDS ECBD_0622 NC_012947.1 640440 641378 D regulates the tdcABCDEFG operon which is involved in amino acid degradation; DNA-binding transcriptional activator TdcA 640440..641378 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158804 YP_003034877.1 CDS ECBD_0623 NC_012947.1 641477 642466 D catalyzes the formation of 2-oxobutanoate from L-threonine; catabolic; threonine dehydratase 641477..642466 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158805 YP_003034878.1 CDS ECBD_0624 NC_012947.1 642488 643819 D involved in the import of threonine and serine in combination with the import of a proton; threonine/serine transporter TdcC 642488..643819 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158806 YP_003034879.1 CDS ECBD_0625 NC_012947.1 643845 645053 D catalyzes the formation of propanoyl phosphate from propanoate and ATP; TdcD also has acetate kinase activities and functions in anaerobic threonine catabolism; propionate/acetate kinase 643845..645053 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158807 YP_003034880.1 CDS ECBD_0626 NC_012947.1 645087 647381 D KEGG: sbc:SbBS512_E3243 formate acetyltransferase; TIGRFAM: formate acetyltransferase; PFAM: pyruvate formate-lyase PFL; formate C-acetyltransferase glycine radical; formate acetyltransferase 645087..647381 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158808 YP_003034881.1 CDS ECBD_0627 NC_012947.1 647395 647784 D TIGRFAM: endoribonuclease L-PSP; PFAM: endoribonuclease L-PSP; KEGG: sbc:SbBS512_E3244 endoribonuclease L-PSP; hypothetical protein 647395..647784 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158809 YP_003034882.1 CDS ECBD_0628 NC_012947.1 647856 649220 D KEGG: sbc:SbBS512_E3245 L-serine ammonia-lyase TdcG; TIGRFAM: L-serine dehydratase; PFAM: serine dehydratase alpha chain; serine dehydratase beta chain; L-serine dehydratase 647856..649220 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158810 YP_003034883.1 CDS ECBD_0629 NC_012947.1 649496 650827 D TIGRFAM: serine transporter; KEGG: ssn:SSON_3268 transporter permease; serine transporter 649496..650827 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158811 YP_003034884.1 CDS ECBD_0630 NC_012947.1 650855 652165 D PFAM: protein of unknown function DUF1063; hypothetical protein 650855..652165 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158812 YP_003034885.1 CDS ECBD_0631 NC_012947.1 652299 652463 R KEGG: sdy:SDY_3299 hypothetical protein; hypothetical protein complement(652299..652463) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158813 YP_003034886.1 CDS ECBD_0632 NC_012947.1 652486 653187 R PFAM: pirin; KEGG: sbc:SbBS512_E3251 pirin family protein; pirin complement(652486..653187) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158814 YP_003034887.1 CDS ECBD_0633 NC_012947.1 653292 654188 D PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: sbo:SBO_2972 transcriptional regulator LysR-type; LysR family transcriptional regulator 653292..654188 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158815 YP_003034888.1 CDS ECBD_0634 NC_012947.1 654239 654595 R PFAM: protein of unknown function DUF805; KEGG: ssn:SSON_3263 cytochrome; hypothetical protein complement(654239..654595) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158816 YP_003034889.1 CDS ECBD_0635 NC_012947.1 654837 655202 R PFAM: protein of unknown function DUF805; KEGG: sbc:SbBS512_E3258 inner membrane protein YhaH; hypothetical protein complement(654837..655202) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158817 YP_003034890.1 CDS ECBD_0636 NC_012947.1 655286 655435 R hypothetical protein complement(655286..655435) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158818 YP_003034891.1 CDS ECBD_0637 NC_012947.1 655495 656481 R KEGG: sdy:SDY_3291 transferase; glutathione transferase complement(655495..656481) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158819 YP_003034892.1 CDS ECBD_0638 NC_012947.1 656551 656943 R PFAM: DoxX family protein; DoxX family protein complement(656551..656943) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158820 YP_003034893.1 CDS ECBD_0639 NC_012947.1 657129 657428 R KEGG: sfv:SFV_3144 hypothetical protein; hypothetical protein complement(657129..657428) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158821 YP_003034894.1 CDS ECBD_0640 NC_012947.1 657418 657822 R KEGG: sbc:SbBS512_E3262 hypothetical protein; hypothetical protein complement(657418..657822) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158822 YP_003034895.1 CDS ECBD_0641 NC_012947.1 657825 658130 R PFAM: protein of unknown function DUF883 ElaB; KEGG: sdy:SDY_3285 hypothetical protein; hypothetical protein complement(657825..658130) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158823 YP_003034896.1 CDS ECBD_0642 NC_012947.1 658168 658536 R PFAM: protein of unknown function DUF1090; KEGG: elf:LF82_3670 protein YqjC; hypothetical protein complement(658168..658536) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158824 YP_003034897.1 CDS ECBD_0643 NC_012947.1 658683 659066 R KEGG: sbc:SbBS512_E3265 hypothetical protein; hypothetical protein complement(658683..659066) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158825 YP_003034898.1 CDS ECBD_0644 NC_012947.1 659070 659732 R PFAM: SNARE associated Golgi protein; KEGG: sdy:SDY_3282 hypothetical protein; hypothetical protein complement(659070..659732) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158662 YP_003034899.1 CDS ECBD_0645 NC_012947.1 659921 660052 R hypothetical protein complement(659921..660052) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158663 YP_003034900.1 CDS ECBD_0646 NC_012947.1 660077 660853 R regulates the exuT, uxaCA and uxuRAB operons which encode genes involved in hexuronate utilization.; DNA-binding transcriptional repressor ExuR complement(660077..660853) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158664 YP_003034901.1 CDS ECBD_0647 NC_012947.1 660983 662281 R TIGRFAM: d-galactonate transporter; PFAM: major facilitator superfamily MFS_1; KEGG: sfv:SFV_3134 transporter of hexuronates; d-galactonate transporter complement(660983..662281) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158665 YP_003034902.1 CDS ECBD_0648 NC_012947.1 662764 664176 D catalyzes the interconversion of D-glucuronate to D-fructuronate or D-galacturonate to D-tagaturonate; functions in glucuronic and galacturonic metabolism; glucuronate isomerase 662764..664176 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158666 YP_003034903.1 CDS ECBD_0649 NC_012947.1 664191 665678 D PFAM: D-galactarate dehydratase/altronate hydrolase domain protein; SAF domain protein; KEGG: sdy:SDY_3276 altronate hydrolase; altronate dehydratase 664191..665678 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158667 YP_003034904.1 CDS ECBD_0650 NC_012947.1 665761 666312 D KEGG: sfv:SFV_3131 hypothetical protein; hypothetical protein 665761..666312 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158668 YP_003034905.1 CDS ECBD_0651 NC_012947.1 666317 667561 R involved in the import of serine and threonine coupled with the import of sodium; serine/threonine transporter SstT complement(666317..667561) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158669 YP_003034906.1 CDS ECBD_0652 NC_012947.1 667766 668068 R KEGG: efe:EFER_1021 hypothetical protein; hypothetical protein complement(667766..668068) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158670 YP_003034907.1 CDS ECBD_0653 NC_012947.1 668177 669142 R PFAM: integral membrane protein TerC; KEGG: ssn:SSON_3117 transporter; integral membrane protein TerC complement(668177..669142) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158671 YP_003034908.1 CDS ECBD_0654 NC_012947.1 669235 669372 R KEGG: spq:SPAB_04023 hypothetical protein; hypothetical protein complement(669235..669372) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158672 YP_003034909.1 CDS ECBD_0655 NC_012947.1 669425 670411 R PFAM: oxidoreductase domain protein; oxidoreductase domain; KEGG: sfv:SFV_3128 hypothetical protein; oxidoreductase domain protein complement(669425..670411) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158673 YP_003034910.1 CDS ECBD_0656 NC_012947.1 670490 671182 R PFAM: protein of unknown function DUF218; KEGG: sfv:SFV_3127 hypothetical protein; hypothetical protein complement(670490..671182) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158674 YP_003034911.1 CDS ECBD_0657 NC_012947.1 671259 671762 R PFAM: protein of unknown function DUF45; KEGG: sdy:SDY_3268 hypothetical protein; hypothetical protein complement(671259..671762) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158675 YP_003034912.1 CDS ECBD_0658 NC_012947.1 671847 672983 D PFAM: methyltransferase small; methyltransferase type 12; KEGG: sdy:SDY_3267 enzyme; rRNA (guanine-N(2)-)-methyltransferase 671847..672983 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158676 YP_003034913.1 CDS ECBD_0659 NC_012947.1 673267 673581 D KEGG: sfv:SFV_3124 hypothetical protein; hypothetical protein 673267..673581 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158677 YP_003034914.1 CDS ECBD_0660 NC_012947.1 673578 673994 D PFAM: helix-turn-helix domain protein; SMART: helix-turn-helix domain protein; KEGG: sfv:SFV_3123 hypothetical protein; XRE family transcriptional regulator 673578..673994 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158678 YP_003034915.1 CDS ECBD_0661 NC_012947.1 674039 676057 R PFAM: NADH:flavin oxidoreductase/NADH oxidase; FAD-dependent pyridine nucleotide-disulphide oxidoreductase; FAD dependent oxidoreductase; KEGG: ssn:SSON_3124 NADPH dehydrogenase; NADH:flavin oxidoreductase/NADH oxidase complement(674039..676057) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158679 YP_003034916.1 CDS ECBD_0662 NC_012947.1 676483 678834 R KEGG: sfl:SF3120 glycosyl hydrolase; glycosyl hydrolase complement(676483..678834) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158680 YP_003034917.1 CDS ECBD_0663 NC_012947.1 678851 679921 R KEGG: sbc:SbBS512_E3514 hypothetical protein; hypothetical protein complement(678851..679921) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158681 YP_003034918.1 CDS ECBD_0664 NC_012947.1 680055 681488 R PFAM: amino acid permease-associated region; KEGG: sbo:SBO_2937 oxidoreductase; amino acid permease-associated region complement(680055..681488) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158682 YP_003034919.1 CDS ebgC NC_012947.1 681551 682000 R in Escherichia coli this is the second beta-galactosidase system; cryptic beta-D-galactosidase subunit beta complement(681551..682000) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158683 YP_003034920.1 CDS ebgA NC_012947.1 681997 685089 R in Escherichia coli this is the second beta-galactosidase system; cryptic beta-D-galactosidase subunit alpha complement(681997..685089) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158684 YP_003034921.1 CDS ECBD_0667 NC_012947.1 685273 686256 R PFAM: regulatory protein LacI; periplasmic binding protein/LacI transcriptional regulator; SMART: regulatory protein LacI; DNA-binding transcriptional repressor EbgR complement(685273..686256) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158685 YP_003034922.1 CDS ECBD_0668 NC_012947.1 686475 686807 D PFAM: t-RNA-binding domain protein; KEGG: sbo:SBO_2933 hypothetical protein; hypothetical protein 686475..686807 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158686 YP_003034923.1 CDS ECBD_0669 NC_012947.1 686849 688138 R catalyzes the formation of 4-aminobutyraldehyde from putrescine and 2-oxoglutarate; putrescine--2-oxoglutarate aminotransferase complement(686849..688138) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158687 YP_003034924.1 CDS ECBD_0670 NC_012947.1 688646 690166 D KEGG: ssn:SSON_3210 aerotaxis sensor receptor, flavoprotein; TIGRFAM: PAS sensor protein; PFAM: chemotaxis sensory transducer; PAS fold-3 domain protein; histidine kinase HAMP region domain protein; SMART: chemotaxis sensory transducer; PAC repeat-containing protein; methyl-accepting chemotaxis sensory transducer with Pas/Pac sensor 688646..690166 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158688 YP_003034925.1 CDS ECBD_0671 NC_012947.1 690320 690943 R PFAM: transcriptional regulator PadR family protein; KEGG: sfv:SFV_3112 hypothetical protein; PadR family transcriptional regulator complement(690320..690943) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158689 YP_003034926.1 CDS ECBD_0672 NC_012947.1 691231 691995 D PFAM: FAD-binding 9 siderophore-interacting domain protein; Siderophore-interacting protein; KEGG: sbc:SbBS512_E3506 siderophore-interacting protein; FAD-binding 9 siderophore-interacting domain protein 691231..691995 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158690 YP_003034927.1 CDS ECBD_0673 NC_012947.1 692249 692755 D PFAM: uracil-DNA glycosylase superfamily; KEGG: sfv:SFV_3109 G/U mismatch-specific DNA glycosylase; G/U mismatch-specific DNA glycosylase 692249..692755 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158692 YP_003034928.1 CDS ECBD_0674 NC_012947.1 692834 694675 R sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; this is the primary sigma factor of bacteria; RNA polymerase sigma factor RpoD complement(692834..694675) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158693 YP_003034929.1 CDS dnaG NC_012947.1 694870 696615 R synthesizes RNA primers at the replication forks; DNA primase complement(694870..696615) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158694 YP_003034930.1 CDS rpsU NC_012947.1 696726 696941 R a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA; 30S ribosomal protein S21 complement(696726..696941) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158695 YP_003034931.1 CDS ECBD_0677 NC_012947.1 697179 698192 D in most organisms, only the N-terminal domain is present in a single polypeptide; in some archaea this domain is fused to a kinase domain; this gene is essential for growth in Escherichia coli and Bacillus subtilis; the secreted glycoprotease from Pasteurella haemolytica showed specificity for O-sialoglycosylated proteins; the Pyrococcus structure shows DNA-binding properties, iron-binding, ATP-binding, and AP endonuclease activity; DNA-binding/iron metalloprotein/AP endonuclease 697179..698192 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158696 YP_003034932.1 CDS ECBD_0678 NC_012947.1 698235 699698 R TIGRFAM: anion transporter; PFAM: sodium/sulfate symporter; KEGG: sfv:SFV_3103 hypothetical protein; anion transporter complement(698235..699698) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158697 YP_003034933.1 CDS ECBD_0679 NC_012947.1 699746 700351 R Involved in the tartrate degradation pathway; L(+)-tartrate dehydratase subunit beta complement(699746..700351) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158698 YP_003034934.1 CDS ECBD_0680 NC_012947.1 700348 701259 R Involved in the tartrate degradation pathway; tartrate dehydratase subunit alpha complement(700348..701259) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158699 YP_003034935.1 CDS ECBD_0681 NC_012947.1 701466 702398 D activates expression of genes required for L-tartrate-dependent anaerobic growth on glycerol; transcriptional activator TtdR 701466..702398 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158700 YP_003034936.1 CDS ECBD_0682 NC_012947.1 702411 703028 R involved in acylation of glycerol-3-phosphate to form 1-acyl-glycerol-3 phosphate for use in phospholipid biosynthesis; functions with PlsX; glycerol-3-phosphate acyltransferase PlsY complement(702411..703028) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158701 YP_003034937.1 CDS folB NC_012947.1 703133 703501 D catalyzes the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin and can also catalyze the epimerization of carbon 2' of dihydroneopterin and dihydromonapterin; bifunctional dihydroneopterin aldolase/dihydroneopterin triphosphate 2'-epimerase 703133..703501 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158702 YP_003034938.1 CDS ECBD_0684 NC_012947.1 703592 704413 D BacA; phosphatase activity in Escherichia coli not kinase; involved in bacitracin resistance as bacitracin supposedly sequesters undecaprenyl disphosphate which reduces the pool of lipid carrier available to the cell; UDP pyrophosphate phosphatase 703592..704413 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158703 YP_003034939.1 CDS cca NC_012947.1 704576 705814 R catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template; phosphohydrolase activities include hydrolysis of pyrophosphate, 5'-nucleoside tri- and diphosphates, NADP, and 2'-AMP with the production of Pi, metal-dependent phosphodiesterase activity for 2',3'-cAMP, 2',3'-cGMP, and 2',3'-cCMP, and hydrolysis 2',3'-cyclic substrates with the formation of 2'-nucleotides and 3'-nucleotides; these phosphohydrolase activities are probably involved in the repair of the tRNA 3'-CCA terminus degraded by intracellular RNases; multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase complement(704576..705814) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158704 YP_003034940.1 CDS ECBD_0686 NC_012947.1 705878 706498 R SMART: SH3 domain protein; KEGG: sfv:SFV_3095 signal transduction protein; signal transduction protein complement(705878..706498) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158705 YP_003034941.1 CDS ECBD_0687 NC_012947.1 706740 708041 D PFAM: adenylate cyclase; CHAD domain containing protein; KEGG: sdy:SDY_3237 hypothetical protein; adenylate cyclase 706740..708041 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158706 YP_003034942.1 CDS ECBD_0688 NC_012947.1 708064 710904 D catalyzes the ATP-dependent addition of AMP to a subunit of glutamine synthetase; also catalyzes the reverse reaction - deadenylation; adenylation/deadenylation of glutamine synthetase subunits is important for the regulation of this enzyme; bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase 708064..710904 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158707 YP_003034943.1 CDS ECBD_0689 NC_012947.1 710952 712385 D catalyzes the phosphorylation of D-glycero-D-manno-heptose 7-phosphate to form D,D-heptose-1,7-bisphosphate and catalyzes transfer of ADP to D-glycero-D-manno-heptose 1-phosphate forming ADP-D,D-heptose; bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase 710952..712385 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158708 YP_003034944.1 CDS ECBD_0690 NC_012947.1 713178 714839 R PFAM: band 7 protein; SMART: band 7 protein; KEGG: ssn:SSON_3188 hypothetical protein; band 7 protein complement(713178..714839) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158709 YP_003034945.1 CDS ECBD_0691 NC_012947.1 714866 715495 R PFAM: protein of unknown function DUF1449; hypothetical protein complement(714866..715495) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158710 YP_003034946.1 CDS ECBD_0692 NC_012947.1 715764 715964 D Involved in glycogen synthesis. May be involved in glycogen priming; glycogen synthesis protein GlgS 715764..715964 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158711 YP_003034947.1 CDS ECBD_0693 NC_012947.1 716007 717071 R KEGG: ssn:SSON_3184 hypothetical protein; hypothetical protein complement(716007..717071) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158712 YP_003034948.1 CDS ECBD_0694 NC_012947.1 717073 717822 R PFAM: Pili assembly chaperone, N-terminal; KEGG: ssn:SSON_3183 hypothetical protein; Pili assembly chaperone, N-terminal complement(717073..717822) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158713 YP_003034949.1 CDS ECBD_0695 NC_012947.1 717838 720351 R PFAM: fimbrial biogenesis outer membrane usher protein; KEGG: elf:LF82_p510 hypothetical outer membrane usher protein YqiG; fimbrial biogenesis outer membrane usher protein complement(717838..720351) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158714 YP_003034950.1 CDS ECBD_0696 NC_012947.1 720402 720953 R PFAM: fimbrial protein; KEGG: ssn:SSON_3180 predicted fimbrial-like adhesin protein; fimbrial protein complement(720402..720953) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158715 YP_003034951.1 CDS ECBD_0697 NC_012947.1 721237 721527 R PFAM: protein of unknown function DUF526; KEGG: sfv:SFV_3086 hypothetical protein; hypothetical protein complement(721237..721527) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158716 YP_003034952.1 CDS ribB NC_012947.1 721901 722554 D DHBP synthase; functions during riboflavin biosynthesis; 3,4-dihydroxy-2-butanone 4-phosphate synthase 721901..722554 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158717 YP_003034953.1 CDS ECBD_0699 NC_012947.1 722816 722986 D KEGG: sbc:SbBS512_E3475 hypothetical protein; hypothetical protein 722816..722986 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158718 YP_003034954.1 CDS ECBD_0700 NC_012947.1 723044 723817 R PFAM: zinc/iron permease; KEGG: ssn:SSON_3177 zinc transporter ZupT; zinc transporter ZupT complement(723044..723817) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158719 YP_003034955.1 CDS ECBD_0701 NC_012947.1 723933 724748 D seems to be involved in biofilm formation; in asymptomatic bacteriuria E. coli strains 83972 and VR50, the ygiD gene is upregulated during biofilm formation in urine; hypothetical protein 723933..724748 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158720 YP_003034956.1 CDS ECBD_0702 NC_012947.1 724786 725946 R PFAM: glutathionylspermidine synthase; KEGG: sbc:SbBS512_E3470 glutathionylspermidine synthase family protein; trypanothione synthase complement(724786..725946) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158721 YP_003034957.1 CDS ECBD_0703 NC_012947.1 725952 726623 R PFAM: protein of unknown function DUF1190; KEGG: sfx:S3282 hypothetical protein; hypothetical protein complement(725952..726623) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158722 YP_003034958.1 CDS tolC NC_012947.1 726771 728252 R trimeric outer membrane protein involved in efflux of hydrophobic/amphipathic molecules; functions with a number of efflux systems in the inner membrane to transport molecules out of the cell; outer membrane channel protein complement(726771..728252) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158723 YP_003034959.1 CDS nudF NC_012947.1 728457 729086 D ADP-sugar pyrophosphatase; catalyzes the formation of D-ribose 5-phosphate from ADP-ribose; can also act on ADP-mannose and ADP-glucose; ADP-ribose pyrophosphatase NudF 728457..729086 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158724 YP_003034960.1 CDS ECBD_0706 NC_012947.1 729087 729509 D PFAM: protein of unknown function DUF1249; KEGG: sfv:SFV_3078 hypothetical protein; hypothetical protein 729087..729509 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158725 YP_003034961.1 CDS ECBD_0707 NC_012947.1 729534 730361 D PFAM: calcineurin phosphoesterase; metallophosphoesterase; KEGG: sdy:SDY_3208 cyclic 3',5'-adenosine monophosphate phosphodiesterase; cyclic 3',5'-adenosine monophosphate phosphodiesterase 729534..730361 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158726 YP_003034962.1 CDS ECBD_0708 NC_012947.1 730361 730942 D Displays esterase activity toward palmitoyl-CoA and pNP-butyrate; esterase YqiA 730361..730942 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158727 YP_003034963.1 CDS ECBD_0709 NC_012947.1 730971 732863 D decatenates newly replicated chromosomal DNA and relaxes positive and negative DNA supercoiling; DNA topoisomerase IV subunit B 730971..732863 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158728 YP_003034964.1 CDS ECBD_0710 NC_012947.1 732911 733225 R PFAM: antibiotic biosynthesis monooxygenase; KEGG: sfv:SFV_3074 hypothetical protein; antibiotic biosynthesis monooxygenase complement(732911..733225) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158729 YP_003034965.1 CDS ECBD_0711 NC_012947.1 733256 733837 R PFAM: NAD(P)H dehydrogenase (quinone); KEGG: ssn:SSON_3166 modulator of drug activity B; NAD(P)H dehydrogenase (quinone) complement(733256..733837) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158730 YP_003034966.1 CDS ECBD_0712 NC_012947.1 734156 734488 D KEGG: sfx:S3272 hypothetical protein; hypothetical protein 734156..734488 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158731 YP_003034967.1 CDS ECBD_0713 NC_012947.1 734534 735883 R PFAM: ATP-binding region ATPase domain protein; histidine kinase HAMP region domain protein; histidine kinase A domain protein; SMART: ATP-binding region ATPase domain protein; histidine kinase A domain protein; KEGG: ssn:SSON_3165 sensor protein QseC; sensor protein QseC complement(734534..735883) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158732 YP_003034968.1 CDS ECBD_0714 NC_012947.1 735880 736539 R response regulator in two-component regulatory system with QseC; regulates FlhCD which is the master regulator for flagella and motility genes; DNA-binding transcriptional regulator QseB complement(735880..736539) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158733 YP_003034969.1 CDS ECBD_0715 NC_012947.1 736691 737083 D PFAM: conserved hypothetical protein; KEGG: sdy:SDY_3215 hypothetical protein; hypothetical protein 736691..737083 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158734 YP_003034970.1 CDS ECBD_0716 NC_012947.1 737136 737618 D PFAM: transcription activator effector binding; KEGG: ssn:SSON_3162 hypothetical protein; transcription activator effector binding 737136..737618 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158735 YP_003034971.1 CDS ECBD_0717 NC_012947.1 737823 738119 D KEGG: yps:YPTB3124 hypothetical protein; hypothetical protein 737823..738119 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158736 YP_003034972.1 CDS ECBD_0718 NC_012947.1 738121 738516 D SMART: helix-turn-helix domain protein; KEGG: ypb:YPTS_3247 XRE family transcriptional regulator; XRE family transcriptional regulator 738121..738516 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158737 YP_003034973.1 CDS ECBD_0719 NC_012947.1 738649 740256 D YgiS; uncharacterized periplasmic protein that seems to be part of a binding-protein-dependent transporter; binding protein 738649..740256 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158738 YP_003034974.1 CDS ECBD_0720 NC_012947.1 740394 742652 D decatenates newly replicated chromosomal DNA and relaxes positive and negative DNA supercoiling; DNA topoisomerase IV subunit A 740394..742652 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158739 YP_003034975.1 CDS ECBD_0721 NC_012947.1 742683 742838 D hypothetical protein 742683..742838 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158740 YP_003034976.1 CDS ECBD_0722 NC_012947.1 742886 743623 D KEGG: sfv:SFV_3067 1-acyl-sn-glycerol-3-phosphate acyltransferase; TIGRFAM: 1-acyl-sn-glycerol-3-phosphate acyltransferase; PFAM: phospholipid/glycerol acyltransferase; SMART: phospholipid/glycerol acyltransferase; 1-acyl-sn-glycerol-3-phosphate acyltransferase 742886..743623 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158741 YP_003034977.1 CDS ECBD_0723 NC_012947.1 743698 745110 D PFAM: multicopper oxidase type 3; multicopper oxidase type 2; KEGG: sbc:SbBS512_E3454 protein SufI; repressor protein for FtsI 743698..745110 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158742 YP_003034978.1 CDS ECBD_0724 NC_012947.1 745221 747440 D PFAM: radical SAM protein; radical SAM domain protein; SMART: Elongator protein 3/MiaB/NifB; hypothetical protein 745221..747440 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158743 YP_003034979.1 CDS ECBD_0725 NC_012947.1 747483 747740 R KEGG: sfv:SFV_3064 outer membrane lipoprotein; outer membrane lipoprotein complement(747483..747740) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159481 YP_003034980.1 CDS ECBD_0726 NC_012947.1 747791 748717 R hypothetical protein complement(747791..748717) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159644 YP_003034981.1 CDS ECBD_0727 NC_012947.1 748802 748915 R KEGG: sbc:SbBS512_E3441 hypothetical protein; hypothetical protein complement(748802..748915) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159645 YP_003034982.1 CDS dkgA NC_012947.1 748917 749744 R methylglyoxal reductase/#-keto ester reductase; catalyzes the reduction of 2,5-diketo-D-gluconic acid to 2-keto-L-gulonic acid; also catalyzes the reduction of methylglyoxal, ethyl-2-methylacetoacetate and ethyl-acetoacetate; 2,5-diketo-D-gluconate reductase A complement(748917..749744) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159646 YP_003034983.1 CDS ECBD_0729 NC_012947.1 749849 751012 R PFAM: iron-containing alcohol dehydrogenase; KEGG: sbc:SbBS512_E3439 alcohol dehydrogenase YqhD; iron-containing alcohol dehydrogenase complement(749849..751012) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159647 YP_003034984.1 CDS ECBD_0730 NC_012947.1 751149 752105 D PFAM: AraC-type transcriptional regulator; helix-turn-helix- domain containing protein AraC type; SMART: helix-turn-helix- domain containing protein AraC type; KEGG: sbc:SbBS512_E3437 AraC family transcriptional regulator; AraC family transcriptional regulator 751149..752105 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159648 YP_003034985.1 CDS ECBD_0731 NC_012947.1 752145 752804 R PFAM: SNARE associated Golgi protein; KEGG: sfv:SFV_3059 hypothetical protein; hypothetical protein complement(752145..752804) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159649 YP_003034986.1 CDS ECBD_0732 NC_012947.1 752944 754131 R catalyzes the formation of L-homocysteine from cystathionine; cystathionine beta-lyase complement(752944..754131) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159650 YP_003034987.1 CDS ECBD_0733 NC_012947.1 754383 755117 D membrane spanning protein in TonB-ExbB-ExbD complex; involved in the tonB-independent energy-dependent transport iron-siderophore complexes and vitamin B12 into the cell; biopolymer transporter ExbB 754383..755117 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159651 YP_003034988.1 CDS ECBD_0734 NC_012947.1 755124 755549 D membrane spanning protein in TonB-ExbB-ExbD complex; involved in the tonB-independent energy-dependent transport iron-siderophore complexes and vitamin B12 into the cell; biopolymer transporter ExbD 755124..755549 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159652 YP_003034989.1 CDS ECBD_0735 NC_012947.1 755821 756705 R PFAM: short-chain dehydrogenase/reductase SDR; KEGG: sbc:SbBS512_E3432 oxidoreductase, short chain dehydrogenase/reductase family; oxidoreductase complement(755821..756705) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159653 YP_003034990.1 CDS ECBD_0736 NC_012947.1 756896 757390 D PFAM: conserved hypothetical protein; KEGG: sbc:SbBS512_E3431 hypothetical protein; hypothetical protein 756896..757390 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159654 YP_003034991.1 CDS ECBD_0737 NC_012947.1 757430 758470 R PFAM: aldo/keto reductase; KEGG: sfv:SFV_3053 aldo-keto reductase; aldo-keto reductase complement(757430..758470) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159655 YP_003034992.1 CDS ECBD_0738 NC_012947.1 758627 759514 D PFAM: dienelactone hydrolase; KEGG: ssn:SSON_3144 predicted hydrolase; carboxymethylenebutenolidase 758627..759514 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159656 YP_003034993.1 CDS ECBD_0739 NC_012947.1 759633 759920 D KEGG: sdy:SDY_3075 hypothetical protein; hypothetical protein 759633..759920 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159657 YP_003034994.1 CDS ECBD_0740 NC_012947.1 759917 760102 D KEGG: sea:SeAg_B3324 hypothetical protein; hypothetical protein 759917..760102 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159658 YP_003034995.1 CDS ECBD_0741 NC_012947.1 760109 761227 D involved in hydrogen uptake; hydrogenase 2 small subunit 760109..761227 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159659 YP_003034996.1 CDS ECBD_0742 NC_012947.1 761230 762216 D Fe-S ferrodoxin type component; participates in the periplasmic electron-transferring activity of hydrogenase 2; hydrogenase 2 protein HybA 761230..762216 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159660 YP_003034997.1 CDS ECBD_0743 NC_012947.1 762206 763384 D HybB; cytochrome b subunit of the hydrogenase 2 enzyme, composed of HybA, B, C, and O subunits; hydrogenase 2 b cytochrome subunit 762206..763384 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159661 YP_003034998.1 CDS ECBD_0744 NC_012947.1 763381 765084 D involved in hydrogen uptake; hydrogenase 2 large subunit 763381..765084 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159662 YP_003034999.1 CDS hybD NC_012947.1 765084 765578 D protease responsible for the cleavage of a 15 amino acid peptide from the C-terminal end of the large subunit of hydrogenase 2 (HybC); hydrogenase 2 maturation endopeptidase 765084..765578 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159663 YP_003035000.1 CDS ECBD_0746 NC_012947.1 765571 766059 D KEGG: sbc:SbBS512_E3419 hydrogenase-2 operon protein HybE; hydrogenase 2-specific chaperone 765571..766059 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159664 YP_003035001.1 CDS ECBD_0747 NC_012947.1 766052 766393 D plays a role in hydrogenase nickel cofactor insertion; hydrogenase nickel incorporation protein HybF 766052..766393 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159665 YP_003035002.1 CDS ECBD_0748 NC_012947.1 766406 766654 D TIGRFAM: hydrogenase assembly chaperone hypC/hupF; PFAM: hydrogenase expression/formation protein (HUPF/HYPC); KEGG: sdy:SDY_3083 hydrogenase 2 accessory protein HypG; hydrogenase 2 accessory protein HypG 766406..766654 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159666 YP_003035003.1 CDS ECBD_0749 NC_012947.1 766777 767643 R YghU; B2989; one of eight glutathione transferase proteins from E. coli; glutathione S-transferase YghU complement(766777..767643) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159667 YP_003035004.1 CDS ECBD_0750 NC_012947.1 767848 769707 D catalyzes the formation of glutathionylspermidine from glutathione and spermidine; also catalyzes the reverse reaction; bifunctional glutathionylspermidine amidase/glutathionylspermidine synthetase 767848..769707 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159668 YP_003035005.1 CDS ECBD_0751 NC_012947.1 769999 771498 D PFAM: phosphate transporter; KEGG: ssn:SSON_3132 low-affinity phosphate transport; phosphate transporter 769999..771498 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159669 YP_003035006.1 CDS ECBD_0752 NC_012947.1 771547 772239 R KEGG: sfx:S3234 hypothetical protein; hypothetical protein complement(771547..772239) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159670 YP_003035007.1 CDS ECBD_0753 NC_012947.1 772428 773126 D KEGG: elf:LF82_3207 uncharacterized ATP-binding protein YghS; ATP-binding protein 772428..773126 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159671 YP_003035008.1 CDS ECBD_0754 NC_012947.1 773158 773916 D hypothetical protein 773158..773916 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159672 YP_003035009.1 CDS ECBD_0755 NC_012947.1 773962 775029 D PFAM: polysaccharide biosynthesis protein; KEGG: elf:LF82_3205 inner membrane protein YghQ; polysaccharide biosynthesis protein 773962..775029 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159673 YP_003035010.1 CDS ECBD_0756 NC_012947.1 775176 776156 R PFAM: transposase IS4 family protein; transposase IS4 family protein complement(775176..776156) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159674 YP_003035011.1 CDS ECBD_0757 NC_012947.1 776191 777327 D hypothetical protein 776191..777327 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159675 YP_003035012.1 CDS ECBD_0758 NC_012947.1 777334 778098 R PFAM: GntR family transcriptional regulator; regulatory protein GntR HTH; SMART: regulatory protein GntR HTH; GlcC family transcriptional regulator complement(777334..778098) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159676 YP_003035013.1 CDS ECBD_0759 NC_012947.1 778349 779848 D TIGRFAM: glycolate oxidase subunit GlcD; PFAM: FAD linked oxidase domain protein; KEGG: elf:LF82_0831 glycolate oxidase subunit GlcD; glycolate oxidase subunit GlcD 778349..779848 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159677 YP_003035014.1 CDS glcE NC_012947.1 779848 780900 D PFAM: FAD linked oxidase; KEGG: elf:LF82_0832 glycolate oxidase subunit GlcE; glycolate oxidase FAD binding subunit 779848..780900 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159678 YP_003035015.1 CDS glcF NC_012947.1 780911 782134 D PFAM: protein of unknown function DUF224 cysteine-rich region domain protein; 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: elf:LF82_0833 glycolate oxidase iron-sulfur subunit; glycolate oxidase iron-sulfur subunit 780911..782134 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159679 YP_003035016.1 CDS ECBD_0762 NC_012947.1 782139 782543 D PFAM: protein of unknown function DUF336; KEGG: sfv:SFV_3032 hypothetical protein; hypothetical protein 782139..782543 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159680 YP_003035017.1 CDS ECBD_0763 NC_012947.1 782565 784736 D catalyzes the formation of malate from glyoxylate and acetyl-CoA; malate synthase G 782565..784736 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159681 YP_003035018.1 CDS ECBD_0764 NC_012947.1 785091 786773 D TIGRFAM: L-lactate transport; PFAM: L-lactate permease; glycolate transporter 785091..786773 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159682 YP_003035019.1 CDS ECBD_0765 NC_012947.1 787258 791820 D KEGG: elf:LF82_3203 lipoprotein AcfD homolog; lipoprotein AcfD-like protein 787258..791820 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159683 YP_003035020.1 CDS ECBD_0766 NC_012947.1 792018 792827 D PFAM: peptidase A24A domain protein; peptidase A24A prepilin type IV; KEGG: efe:EFER_2912 bifunctional prepilin leader peptidase and methylase; prepilin peptidase 792018..792827 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159684 YP_003035021.1 CDS ECBD_0767 NC_012947.1 792893 793303 D KEGG: elf:LF82_3202 uncharacterized lipoprotein YghG; hypothetical protein 792893..793303 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159685 YP_003035022.1 CDS ECBD_0768 NC_012947.1 793321 794316 D part of the general (type II) secretion pathway (GSP), a signal sequence-dependent process responsible for protein export; involved in trans-location of exoproteins from the cytoplasm to the periplasm; type II secretion protein GspC 793321..794316 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159686 YP_003035023.1 CDS ECBD_0769 NC_012947.1 794382 795242 D TIGRFAM: general secretion pathway protein L; PFAM: general secretion pathway L; KEGG: sbc:SbBS512_E3404 general secretion pathway protein GspL; general secretion pathway protein L 794382..795242 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159687 YP_003035024.1 CDS ECBD_0770 NC_012947.1 795244 795780 D PFAM: general secretion pathway M protein; KEGG: sbc:SbBS512_E3403 general secretion pathway protein M; general secretion pathway protein YghD 795244..795780 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159688 YP_003035025.1 CDS ECBD_0771 NC_012947.1 796110 796817 R PFAM: protein of unknown function DUF554; KEGG: ssn:SSON_3229 transporter; hypothetical protein complement(796110..796817) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159690 YP_003035026.1 CDS ECBD_0772 NC_012947.1 797215 799350 D PFAM: Orn/Lys/Arg decarboxylase major region; Orn/Lys/Arg decarboxylase domain protein; KEGG: elf:LF82_2159 ornithine decarboxylase, constitutive; ornithine decarboxylase 797215..799350 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159691 YP_003035027.1 CDS ECBD_0774 NC_012947.1 800015 801223 R PFAM: transposase IS4 family protein; KEGG: seh:SeHA_C4716 transposase (IS4 family); transposase IS4 family protein complement(800015..801223) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159693 YP_003035028.1 CDS ECBD_0775 NC_012947.1 801353 802561 R PFAM: transposase IS4 family protein; KEGG: apa:APP7_1819 iso-IS10R ORF; transposase IS4 family protein complement(801353..802561) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159694 YP_003035029.1 CDS mltC NC_012947.1 803534 804616 R Murein hydrolase C; membrane-bound; lytic; catalyzes the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues; murein transglycosylase C complement(803534..804616) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159695 YP_003035030.1 CDS ECBD_0777 NC_012947.1 804678 804953 R PFAM: Fe(II) trafficking protein YggX; KEGG: sfv:SFV_3020 hypothetical protein; hypothetical protein complement(804678..804953) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159696 YP_003035031.1 CDS ECBD_0778 NC_012947.1 804981 806033 R KEGG: sbo:SBO_3029 adenine DNA glycosylase; TIGRFAM: A/G-specific adenine glycosylase; PFAM: HhH-GPD family protein; helix-hairpin-helix motif; SMART: HhH-GPD family protein; iron-sulfur cluster loop; adenine DNA glycosylase complement(804981..806033) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159697 YP_003035032.1 CDS trmB NC_012947.1 806194 806913 D tRNA (guanine-N(7)-)-methyltransferase; catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA by transferring the methyl residue from S-adenosyl-L-methionine; tRNA (guanine-N(7)-)-methyltransferase 806194..806913 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159698 YP_003035033.1 CDS ECBD_0780 NC_012947.1 806913 807239 D PFAM: protein of unknown function DUF469; KEGG: sdy:SDY_3113 hypothetical protein; hypothetical protein 806913..807239 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159699 YP_003035034.1 CDS ECBD_0781 NC_012947.1 807262 807402 D KEGG: sbc:SbBS512_E3391 hypothetical protein; hypothetical protein 807262..807402 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159700 YP_003035035.1 CDS ECBD_0782 NC_012947.1 807423 808142 D KEGG: sdy:SDY_3114 hypothetical protein; hypothetical protein 807423..808142 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159701 YP_003035036.1 CDS ansB NC_012947.1 808318 809364 D catalyzes the formation of aspartate from asparagine, periplasmic; regulated by cyclic AMP receptor protein (CRP) and also induced by anaerobiosis; L-asparaginase II 808318..809364 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159702 YP_003035037.1 CDS ECBD_0784 NC_012947.1 809481 810488 D PFAM: protein of unknown function DUF1202; KEGG: sbc:SbBS512_E3388 hypothetical protein; hypothetical protein 809481..810488 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159703 YP_003035038.1 CDS ECBD_0785 NC_012947.1 810643 811779 R catalyzes the oxygen-independent formation of protoporphyrinogen-IX from coproporphyrinogen-III; coproporphyrinogen III oxidase complement(810643..811779) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159704 YP_003035039.1 CDS ECBD_0786 NC_012947.1 811772 812365 R HAM1-like protein; Rec-dependent growth; RgdB; yggV; it is suspected that this protein functions to remove misincorporated bases such as xanthine or hypoxanthine; deoxyribonucleotide triphosphate pyrophosphatase complement(811772..812365) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159705 YP_003035040.1 CDS ECBD_0787 NC_012947.1 812373 812663 R PFAM: protein of unknown function DUF167; KEGG: sbc:SbBS512_E3385 hypothetical protein; hypothetical protein complement(812373..812663) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159706 YP_003035041.1 CDS ECBD_0788 NC_012947.1 812660 813226 R PFAM: protein of unknown function YGGT; KEGG: sdy:SDY_3120 resistance protein; hypothetical protein complement(812660..813226) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159707 YP_003035042.1 CDS ECBD_0789 NC_012947.1 813244 813948 R PFAM: alanine racemase domain protein; KEGG: sfv:SFV_3005 hypothetical protein; alanine racemase domain protein complement(813244..813948) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159708 YP_003035043.1 CDS ECBD_0790 NC_012947.1 813966 814946 D KEGG: sfv:SFV_3004 transporter; TIGRFAM: twitching motility protein; PFAM: type II secretion system protein E; SMART: ATPase AAA; twitching motility protein 813966..814946 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159709 YP_003035044.1 CDS ECBD_0791 NC_012947.1 815130 815546 R similar to RuvC resolvase with substantial differences; NMR structural information suggests this protein is monomeric; unknown cellular function; Holliday junction resolvase-like protein complement(815130..815546) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159710 YP_003035045.1 CDS ECBD_0792 NC_012947.1 815546 816181 R PFAM: protein of unknown function DUF179; KEGG: sfv:SFV_3002 hypothetical protein; hypothetical protein complement(815546..816181) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159711 YP_003035046.1 CDS ECBD_0793 NC_012947.1 816218 817168 R catalyzes the second step in the glutathione biosynthesis pathway, where it synthesizes ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione; glutathione synthetase complement(816218..817168) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159712 YP_003035047.1 CDS ECBD_0794 NC_012947.1 817181 817912 R in Escherichia coli RsmE methylates the N3 position of the U1498 base in 16S rRNA; cells lacking this function can grow, but are outcompeted by wild-type; SAM-dependent m(3)U1498 methyltransferase; 16S ribosomal RNA methyltransferase RsmE complement(817181..817912) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159713 YP_003035048.1 CDS ECBD_0795 NC_012947.1 817992 818699 R PFAM: endonuclease I; KEGG: sbc:SbBS512_E3377 endonuclease I; deoxyribonuclease I complement(817992..818699) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159714 YP_003035049.1 CDS ECBD_0796 NC_012947.1 818794 819291 R PFAM: protein of unknown function DUF335 SprT; SMART: protein of unknown function SprT; KEGG: sbo:SBO_3046 hypothetical protein; hypothetical protein complement(818794..819291) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159715 YP_003035050.1 CDS ECBD_0797 NC_012947.1 819368 820762 R TIGRFAM: sugar transporter; PFAM: general substrate transporter; major facilitator superfamily MFS_1; KEGG: sdy:SDY_3129 galactose-proton symport of transporter; sugar transporter complement(819368..820762) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159716 YP_003035051.1 CDS ECBD_0798 NC_012947.1 821186 822340 R catalyzes the formation of S-adenosylmethionine from methionine and ATP; methionine adenosyltransferase; S-adenosylmethionine synthetase complement(821186..822340) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159717 YP_003035052.1 CDS ECBD_0799 NC_012947.1 822396 822647 D KEGG: sfv:SFV_2995 hypothetical protein; hypothetical protein 822396..822647 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159718 YP_003035053.1 CDS ECBD_0800 NC_012947.1 823135 825111 D catalyzes the formation of agmatine from arginine in putrescine and spermidine biosynthesis; arginine decarboxylase 823135..825111 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159719 YP_003035054.1 CDS ECBD_0801 NC_012947.1 825249 826169 D catalyzes the formation of putrescine from agmatine; agmatinase 825249..826169 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159720 YP_003035055.1 CDS ECBD_0802 NC_012947.1 826375 827133 R PFAM: peptidase M48 Ste24p; KEGG: sbc:SbBS512_E3369 peptidase, M48B family; peptidase M48 Ste24p complement(826375..827133) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159721 YP_003035056.1 CDS ECBD_0803 NC_012947.1 827411 829402 D catalyzes the formation of ribose 5-phosphate and xylulose 5-phosphate from sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate; can transfer ketol groups between several groups; in Escherichia coli there are two tkt genes, tktA expressed during exponential growth and the tktB during stationary phase; transketolase 827411..829402 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159722 YP_003035057.1 CDS ECBD_0804 NC_012947.1 829536 829706 D hypothetical protein 829536..829706 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159723 YP_003035058.1 CDS cmtB NC_012947.1 829716 830159 D TolM; with CmtA (IIBC), CmtB possibly forms the mannitol-like permease of the cryptic mannitol phosphotransferase system, which phosphorylates and transports various carbohydrates and polyhydric alcohols in Escherichia coli; cytoplasmic protein; mannitol phosphotransferase subunit EIIA 829716..830159 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159724 YP_003035059.1 CDS ECBD_0806 NC_012947.1 830187 831575 D TIGRFAM: PTS system, mannitol-specific IIC subunit; PFAM: phosphotransferase system EIIC; phosphotransferase system lactose/cellobiose-specific IIB subunit; KEGG: sbc:SbBS512_E3365 PTS system, mannitol-specific cryptic EIICB component; PTS system, mannitol-specific IIC subunit 830187..831575 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159726 YP_003035060.1 CDS ECBD_0807 NC_012947.1 831590 832867 D PFAM: alcohol dehydrogenase zinc-binding domain protein; alcohol dehydrogenase GroES domain protein; alcohol dehydrogenase zinc-binding domain protein 831590..832867 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159727 YP_003035061.1 CDS ECBD_0808 NC_012947.1 832864 833829 D KEGG: sfv:SFV_2982 fructose-1,6-bisphosphatase II-like protein; TIGRFAM: fructose-1,6-bisphosphatase, class II; PFAM: GlpX family protein; fructose-1,6-bisphosphatase II-like protein 832864..833829 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159728 YP_003035062.1 CDS ECBD_0809 NC_012947.1 833851 834360 D PFAM: Mannitol repressor; KEGG: sbo:SBO_3059 DNA-binding transcriptional regulator; DNA-binding transcriptional regulator 833851..834360 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159729 YP_003035063.1 CDS frcK NC_012947.1 834357 835070 D KEGG: efe:EFER_2860 conserved hypothetical protein; nucleoside triphosphate hydrolase domain; fructose transporter kinase 834357..835070 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159730 YP_003035064.1 CDS ECBD_0811 NC_012947.1 835355 836374 D NAD-dependent; catalyzes the formation of 4-phosphoerythronate from erythrose 4-phosphate in the biosynthesis of pyridoxine 5'-phosphate; erythrose 4-phosphate dehydrogenase 835355..836374 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159731 YP_003035065.1 CDS pgk NC_012947.1 836424 837587 D Converts 3-phospho-D-glycerate to 3-phospho-D-glyceroyl phosphate during the glycolysis pathway; phosphoglycerate kinase 836424..837587 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159732 YP_003035066.1 CDS ECBD_0813 NC_012947.1 837802 838881 D catalyzes the formation of glycerone phosphate and glyceraldehyde 3-phosphate from fructose 1,6, bisphosphate; fructose-bisphosphate aldolase 837802..838881 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159733 YP_003035067.1 CDS ECBD_0814 NC_012947.1 839239 840099 D participates in the regulation of osmotic pressure changes within the cel; mechanosensitive channel MscS 839239..840099 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159734 YP_003035068.1 CDS ECBD_0815 NC_012947.1 840238 840873 D Involved in the export of arginine; arginine exporter protein 840238..840873 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159735 YP_003035069.1 CDS ECBD_0816 NC_012947.1 840966 841706 D PFAM: protein of unknown function DUF541; KEGG: sdy:SDY_3160 hypothetical protein; hypothetical protein 840966..841706 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159736 YP_003035070.1 CDS ECBD_0817 NC_012947.1 841873 842769 D PFAM: regulatory protein LysR; LysR substrate-binding; KEGG: sbc:SbBS512_E3344 transcriptional regulator, LysR family; LysR family transcriptional regulator 841873..842769 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159737 YP_003035071.1 CDS ECBD_0818 NC_012947.1 842766 844244 R KEGG: ssn:SSON_3071 coenzyme A transferase; TIGRFAM: succinate CoA transferase; PFAM: acetyl-CoA hydrolase/transferase; succinate CoA transferase complement(842766..844244) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159738 YP_003035072.1 CDS ECBD_0819 NC_012947.1 844268 845053 R catalyzes the formation of propanoyl-CoA from methylmalonyl-CoA; methylmalonyl-CoA decarboxylase complement(844268..845053) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159739 YP_003035073.1 CDS ECBD_0820 NC_012947.1 845064 846059 R functions in transport of arginine/ornithine; inner membrane ATPase that cleaves ATP and phosphorylates two periplasmic proteins that function as two distinct transporters, the AO (arginine and ornithine) and LAO (lysine, arginine, and ornithine) periplasmic binding proteins; arginine/ornithine transporter ATPase complement(845064..846059) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159740 YP_003035074.1 CDS ECBD_0821 NC_012947.1 846052 848196 R MDM; functions in conversion of succinate to propionate; methylmalonyl-CoA mutase complement(846052..848196) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159741 YP_003035075.1 CDS ECBD_0822 NC_012947.1 848400 849293 R specific inhibitor of chromosomal initiation of replication in vitro; binds the three 13-mers in the origin (oriC) to block initiation of replication; also controls genes involved in arginine transport; chromosome replication initiation inhibitor protein complement(848400..849293) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159742 YP_003035076.1 CDS ECBD_0823 NC_012947.1 849435 849665 D KEGG: elf:LF82_3651 uncharacterized protein YqfE; hypothetical protein 849435..849665 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159743 YP_003035077.1 CDS ECBD_0824 NC_012947.1 849721 850380 D Catalyzes D-ribose 5-phosphate --> D-ribulose 5-phosphate in the nonoxidative branch of the pentose phosphate pathway; ribose-5-phosphate isomerase A 849721..850380 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159744 YP_003035078.1 CDS ECBD_0825 NC_012947.1 850636 851868 D catalyzes the formation of 3-phosphonooxypyruvate from 3-phospho-D-glycerate in serine biosynthesis; can also reduce alpha ketoglutarate to form 2-hydroxyglutarate; D-3-phosphoglycerate dehydrogenase 850636..851868 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159745 YP_003035079.1 CDS ECBD_0826 NC_012947.1 852257 852859 R ygfA expression increases five to eight fold in Escherichia coli cells growing as biofilms; unknown function; ligase complement(852257..852859) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159746 YP_003035080.1 CDS ECBD_0827 NC_012947.1 853105 853434 R PFAM: protein of unknown function DUF710; KEGG: sfv:SFV_2958 Z-ring-associated protein; Z-ring-associated protein complement(853105..853434) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159748 YP_003035081.1 CDS ECBD_0828 NC_012947.1 853596 854180 D the crystal structure of Haemophilus influenzae HI0817 showed that this protein forms dimers; function unknown; hypothetical protein 853596..854180 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159749 YP_003035082.1 CDS ECBD_0829 NC_012947.1 854206 855531 D exopeptidase able to cleave the peptide bond of the last amino acid if linked to a proline residue; substrate can be as short as a dipeptide; proline aminopeptidase P II 854206..855531 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159750 YP_003035083.1 CDS ECBD_0830 NC_012947.1 855528 856706 D Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone; 2-octaprenyl-6-methoxyphenyl hydroxylase 855528..856706 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159751 YP_003035084.1 CDS ECBD_0831 NC_012947.1 856729 857931 D TIGRFAM: Ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family; PFAM: monooxygenase FAD-binding; FAD dependent oxidoreductase; KEGG: ssn:SSON_3059 hypothetical protein; hypothetical protein 856729..857931 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159752 YP_003035085.1 CDS gcvT NC_012947.1 858378 859472 D catalyzes the transfer of a methylene carbon from the methylamine-loaded GcvH protein to tetrahydrofolate, causing the release of ammonia and the generation of reduced GcvH protein; glycine cleavage system aminomethyltransferase T 858378..859472 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159753 YP_003035086.1 CDS ECBD_0833 NC_012947.1 859496 859885 D part of multienzyme complex composed of H, L, P, and T proteins which catalyzes oxidation of glycine to yield carbon dioxide, ammonia, 5,10-CH2-H4folate and a reduced pyridine nucleotide; protein H is involved in transfer of methylamine group from the P to T protein; covalently bound to a lipoyl cofactor; glycine cleavage system protein H 859496..859885 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159754 YP_003035087.1 CDS ECBD_0834 NC_012947.1 860004 862877 D acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine; glycine dehydrogenase 860004..862877 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159755 YP_003035088.1 CDS ECBD_0835 NC_012947.1 863143 863886 D PFAM: short-chain dehydrogenase/reductase SDR; KR domain protein; NAD-dependent epimerase/dehydratase; KEGG: sbc:SbBS512_E3323 NAD dependent epimerase/dehydratase family; hypothetical protein 863143..863886 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159756 YP_003035089.1 CDS ECBD_0836 NC_012947.1 863943 865376 R PFAM: glycoside hydrolase family 1; KEGG: sbo:SBO_3091 6-phospho-beta-glucosidase A; cryptic; glycoside hydrolase family 1 complement(863943..865376) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159757 YP_003035090.1 CDS ECBD_0837 NC_012947.1 865421 865732 D PFAM: protein of unknown function DUF437; KEGG: ssn:SSON_3053 hypothetical protein; hypothetical protein 865421..865732 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159758 YP_003035091.1 CDS ECBD_0838 NC_012947.1 865896 866555 D TIGRFAM: channel protein, hemolysin III family; PFAM: Hly-III family protein; KEGG: sbc:SbBS512_E3320 channel protein, hemolysin III family; channel protein, hemolysin III family 865896..866555 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159759 YP_003035092.1 CDS ECBD_0839 NC_012947.1 866751 867731 R physiological role is not clear; not essential for growth but mutants grow poorly; mutations suppress hda null mutations; may be involved in regulation of ATP-DnaA levels; may also be involved in regulation of tRNA modifications; can bind folic acid and tetrahydrofolate; forms a three domain ring-like structure with a central channel; structurally similar to DMGO protein from A. globiformis; shows sequence similarity to yeast CAF17 transcriptional regulator; global regulator complement(866751..867731) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159760 YP_003035093.1 CDS ECBD_0840 NC_012947.1 867974 868240 D PFAM: protein of unknown function DUF339; KEGG: sbo:SBO_3095 hypothetical protein; hypothetical protein 867974..868240 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159761 YP_003035094.1 CDS ECBD_0841 NC_012947.1 868221 868628 D PFAM: protein of unknown function DUF1434; KEGG: sbc:SbBS512_E3316 hypothetical protein; hypothetical protein 868221..868628 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159762 YP_003035095.1 CDS ECBD_0842 NC_012947.1 868668 869189 R An electron-transfer protein; flavodoxin binds one FMN molecule, which serves as a redox-active prosthetic; flavodoxin FldB complement(868668..869189) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159763 YP_003035096.1 CDS xerD NC_012947.1 869301 870197 D site-specific tyrosine recombinase which cuts and rejoins DNA molecules; binds cooperatively to specific DNA consensus sites; forms a heterotetrameric complex with XerC; XerCD exhibit similar sequences; essential to convert chromosome dimers to monomers during cell division and functions during plasmid segregation; XerD specifically exchanges the bottom strands; cell division protein FtsK may regulate the XerCD complex; enzyme from Streptococcus group has unusual active site motifs; site-specific tyrosine recombinase XerD 869301..870197 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159764 YP_003035097.1 CDS ECBD_0844 NC_012947.1 870222 870932 D DsbC; periplasmic protein with protein disulfide isomerase activity; this protein also shows disulfide oxidoreductase activity; thiol:disulfide interchange protein DsbC 870222..870932 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159765 YP_003035098.1 CDS ECBD_0845 NC_012947.1 870938 872671 D 5'-3' single-stranded-DNA-specific exonuclease; ssDNA exonuclease RecJ 870938..872671 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159766 YP_003035099.1 CDS prfB NC_012947.1 872762 873860 D recognizes the termination signals UGA and UAA during protein translation a specificity which is dependent on amino acid residues residing in loops of the L-shaped tRNA-like molecule of RF2; in some organisms control of PrfB protein levels is maintained through a +1 ribosomal frameshifting mechanism; this protein is similar to release factor 1; peptide chain release factor 2 join(872762..872836,872838..873860) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159767 YP_003035100.1 CDS lysS NC_012947.1 873870 875387 D class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA molecules for lysine that exist in this organism and in addition can charge the tRNAPyl with lysine in the presence of LysRS1; lysyl-tRNA synthetase 873870..875387 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159768 YP_003035101.1 CDS ECBD_0848 NC_012947.1 875430 875978 R catalyzes the rearrangement of isopentenyl diphosphate to dimethylallyl phosphate; isopentenyl-diphosphate delta-isomerase complement(875430..875978) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159769 YP_003035102.1 CDS ECBD_0849 NC_012947.1 876101 876226 R KEGG: sbc:SbBS512_E3307 hypothetical protein; hypothetical protein complement(876101..876226) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159770 YP_003035103.1 CDS ECBD_0850 NC_012947.1 876228 877676 R TIGRFAM: xanthine permease; uracil-xanthine permease; PFAM: xanthine/uracil/vitamin C permease; xanthine permease complement(876228..877676) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159771 YP_003035104.1 CDS ECBD_0851 NC_012947.1 878112 880031 D unknown function; in E. coli the aegA gene is regulated by Fnr, NarXL, and NarQ (but not ArcA), induced under anaerobic conditions and repressed in the presence of nitrate (anaerobic); oxidoreductase Fe-S binding subunit 878112..880031 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159772 YP_003035105.1 CDS ECBD_0852 NC_012947.1 880031 880519 D PFAM: 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: ssn:SSON_3038 oxidoreductase, Fe-S subunit; 4Fe-4S ferredoxin iron-sulfur binding domain protein 880031..880519 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159773 YP_003035106.1 CDS ECBD_0853 NC_012947.1 880555 881922 R PFAM: xanthine/uracil/vitamin C permease; sulfate transporter; xanthine/uracil/vitamin C permease complement(880555..881922) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159774 YP_003035107.1 CDS ECBD_0854 NC_012947.1 881958 883274 R Catalyzes the deamination of guanine; guanine deaminase complement(881958..883274) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159775 YP_003035108.1 CDS ECBD_0855 NC_012947.1 883292 884692 R TIGRFAM: uracil-xanthine permease; xanthine permease; PFAM: xanthine/uracil/vitamin C permease; uracil-xanthine permease complement(883292..884692) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159776 YP_003035109.1 CDS ECBD_0856 NC_012947.1 884857 887727 R with YgfK and YgfM forms a selenate reductase, which seems to catalyze the reduction of selenate to selenite; molybdopterin-containing enzyme; oxidase activity toward hypoxanthine and xanthine can be detected if these substrates are present at high concentration; selenate reductase subunit YgfN complement(884857..887727) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159777 YP_003035110.1 CDS ECBD_0857 NC_012947.1 887724 888503 R with YgfK and YgfN forms a selenate reductase, which seems to catalyze the reduction of selenate to selenite; YgfM contains a FAD domain; selenate reductase subunit YgfM complement(887724..888503) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159778 YP_003035111.1 CDS ECBD_0858 NC_012947.1 888554 889882 R in Escherichia coli expression is induced in response to phase-specific signals and controlled by RpoS; chlorohydrolase/aminohydrolase complement(888554..889882) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159779 YP_003035112.1 CDS ECBD_0859 NC_012947.1 889885 892983 R with YgfM and YgfN forms a selenate reductase, which seems to catalyze the reduction of selenate to selenite; YgfK mutants are unable to reduce selenate; involved in purine salvage process; seems to act as an oxidoreductase, but sequence similarity suggests it is a dihydrothymine dehydrogenase; selenate reductase subunit YgfK complement(889885..892983) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159780 YP_003035113.1 CDS ECBD_0860 NC_012947.1 893304 893882 R TIGRFAM: molybdenum hydroxylase accessory protein, YgfJ family; KEGG: elf:LF82_3171 uncharacterized protein YgfJ; molybdenum hydroxylase accessory protein, YgfJ family complement(893304..893882) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159781 YP_003035114.1 CDS ECBD_0861 NC_012947.1 893985 894755 D hypothetical protein 893985..894755 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159782 YP_003035115.1 CDS ECBD_0862 NC_012947.1 894803 896428 D TIGRFAM: selenium-dependent molybdenum hydroxylase system protein, YqeB family; PFAM: protein of unknown function DUF182; biotin/lipoyl attachment domain-containing protein; KEGG: sbo:SBO_3109 synthase; selenium-dependent molybdenum hydroxylase system protein, YqeB family 894803..896428 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159783 YP_003035116.1 CDS ECBD_0863 NC_012947.1 896649 897581 R catalyzes the reversible synthesis of carbamate and ATP from carbamoyl phosphate and ADP; carbamate kinase complement(896649..897581) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159784 YP_003035117.1 CDS ECBD_0864 NC_012947.1 897629 899014 R catalyzes the hydrolytic cleavage of hydantoin with aromatic side chains at the 5'position; phenylhydantoinase complement(897629..899014) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159785 YP_003035118.1 CDS ECBD_0865 NC_012947.1 899067 900278 R Zn-dependent extracellular dipeptidase; similar to succinyl-diaminopimelate desuccinylases; peptidase complement(899067..900278) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159786 YP_003035119.1 CDS ECBD_0866 NC_012947.1 900336 901532 R catalyzes the formation of pyruvate from 2,3-diaminopropionate; diaminopropionate ammonia-lyase complement(900336..901532) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159787 YP_003035120.1 CDS ECBD_0867 NC_012947.1 901590 902777 R TIGRFAM: carbamoyltransferase YgeW; PFAM: aspartate/ornithine carbamoyltransferase carbamoyl-P binding domain; aspartate/ornithine carbamoyltransferase Asp/Orn-binding region; KEGG: elf:LF82_3165 uncharacterized protein YgeW; aspartate/ornithine carbamoyltransferase family protein complement(901590..902777) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159788 YP_003035121.1 CDS ECBD_0868 NC_012947.1 903256 905034 D PFAM: sigma-54 factor interaction domain-containing protein; helix-turn-helix Fis-type; SMART: ATPase AAA; PAS domain containing protein; KEGG: ssn:SSON_3020 transcriptional regulator; PAS modulated sigma54 specific transcriptional regulator, Fis family 903256..905034 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159789 YP_003035122.1 CDS ECBD_0869 NC_012947.1 905074 905553 R iron-sulfur-binding subunit; with XdhA and XdhB participates in purine salvage; xanthine dehydrogenase subunit XdhC complement(905074..905553) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159790 YP_003035123.1 CDS ECBD_0870 NC_012947.1 905550 906428 R FAD-binding subunit; with XdhA and XdhC participates in purine salvage; xanthine dehydrogenase subunit XdhB complement(905550..906428) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159791 YP_003035124.1 CDS ECBD_0871 NC_012947.1 906439 908736 R molybdenum cofactor-binding protein; participates in purine salvage; xanthine dehydrogenase subunit XdhA complement(906439..908736) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159792 YP_003035125.1 CDS ECBD_0872 NC_012947.1 909151 909906 D PFAM: peptidase M23; peptidoglycan-binding LysM; SMART: peptidoglycan-binding LysM; KEGG: elf:LF82_3163 uncharacterized lipoprotein YgeR; peptidase M23 909151..909906 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159793 YP_003035126.1 CDS ECBD_0873 NC_012947.1 910296 910847 D KEGG: sfv:SFV_2926 hypothetical protein; hypothetical protein 910296..910847 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159795 YP_003035127.1 CDS ECBD_0874 NC_012947.1 910861 911727 R PFAM: Integrase catalytic region; KEGG: sfv:SFV_1039 IS3 ORF2; Integrase catalytic region complement(910861..911727) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159796 YP_003035128.1 CDS ECBD_0875 NC_012947.1 911724 912023 R PFAM: transposase IS3/IS911 family protein; transposase IS3/IS911 family protein complement(911724..912023) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159797 YP_003035129.1 CDS ECBD_0876 NC_012947.1 912070 912639 R KEGG: ssn:SSON_3006 hypothetical protein; hypothetical protein complement(912070..912639) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159798 YP_003035130.1 CDS ECBD_0877 NC_012947.1 913099 914328 R TIGRFAM: serine transporter; PFAM: Amino acid transporter transmembrane; KEGG: elf:LF82_3648 inner membrane transporter YqeG; serine transporter complement(913099..914328) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159799 YP_003035131.1 CDS ECBD_0878 NC_012947.1 914584 915765 D Catalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation; in Rhizobia and Ralstonia is involved in PHB biosynthesis; acetyl-CoA acetyltransferase 914584..915765 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159800 YP_003035132.1 CDS ECBD_0879 NC_012947.1 916052 916888 D 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase; catalyzes the interconversion of 4-deoxy-L-threo-5-hexosulose uronate to 3-deoxy-D-glycero-2,5-hexodiulosonate; 5-keto-4-deoxyuronate isomerase 916052..916888 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159801 YP_003035133.1 CDS ECBD_0881 NC_012947.1 917511 918362 R PFAM: Integrase catalytic region; KEGG: sfv:SFV_3530 IS150 ORF2; Integrase catalytic region complement(917511..918362) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159803 YP_003035134.1 CDS ECBD_0882 NC_012947.1 918359 918880 R KEGG: sfx:S1483 IS103 orf; IS103 orf complement(918359..918880) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159804 YP_003035135.1 CDS ECBD_0883 NC_012947.1 919440 920858 D TIGRFAM: sugar transporter; PFAM: general substrate transporter; major facilitator superfamily MFS_1; KEGG: ssn:SSON_3001 low-affinity L-arabinose transporter proton symport protein; sugar transporter 919440..920858 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156698 YP_003035136.1 CDS ECBD_0884 NC_012947.1 920987 921679 D TIGRFAM: aspartate racemase; PFAM: Asp/Glu/hydantoin racemase; KEGG: sbc:SbBS512_E3023 Asp/Glu/hydantoin racemase; racemase 920987..921679 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156699 YP_003035137.1 CDS ECBD_0885 NC_012947.1 921666 922601 R activates the transcription of the lysA gene encoding diaminopimelate decarboxylase; LysR family transcriptional regulator complement(921666..922601) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156700 YP_003035138.1 CDS ECBD_0886 NC_012947.1 922723 923985 D catalyzes the decarboxylation of diaminopimelate into lysine; pyridoxal phosphate(PLP)-binding; diaminopimelate decarboxylase 922723..923985 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156701 YP_003035139.1 CDS ECBD_0887 NC_012947.1 923992 925023 R controls transcription of galETKM; DNA-binding transcriptional regulator GalR complement(923992..925023) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156702 YP_003035140.1 CDS ECBD_0888 NC_012947.1 925609 927768 D transfers fatty acids to the 1-position via an enzyme-bound acyl-ACP intermediate in the presence of ATP and magnesium. Its physiological function is to regenerate phosphatidylethanolamine from 2-acyl-glycero-3-phosphoethanolamine (2-acyl-GPE) formed by transacylation reactions or degradation by phospholipase A1; bifunctional acyl-[acyl carrier protein] synthetase/2-acylglycerophosphoethanolamine acyltransferase 925609..927768 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156703 YP_003035141.1 CDS ECBD_0889 NC_012947.1 927761 928954 D PFAM: major facilitator superfamily MFS_1; KEGG: sbc:SbBS512_E3027 lysophospholipid transporter; lysophospholipid transporter LplT 927761..928954 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156704 YP_003035142.1 CDS tas NC_012947.1 928986 930026 R complements the prephenate dehydrogenase dysfunction of an Escherichia coli tyrosine auxotroph strain; member of the aldo/keto reductase 2 family; crystal structure of purified Escherichia coli protein shows bound NADPH; aldo-keto reductase complement(928986..930026) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156705 YP_003035143.1 CDS ECBD_0891 NC_012947.1 930134 930352 R PFAM: protein of unknown function DUF903; KEGG: ssn:SSON_2990 hypothetical protein; hypothetical protein complement(930134..930352) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156706 YP_003035144.1 CDS ECBD_0892 NC_012947.1 930490 931203 R PFAM: integral membrane protein TerC; KEGG: sdy:SDY_3049 transporter; integral membrane protein TerC complement(930490..931203) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156707 YP_003035145.1 CDS ECBD_0893 NC_012947.1 931272 931961 R MutH; Sequence-specific endonuclease that cleaves unmethylated GATC sequences during DNA repair; DNA mismatch repair protein complement(931272..931961) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156708 YP_003035146.1 CDS ECBD_0894 NC_012947.1 932147 932293 D KEGG: elf:LF82_p452 hypothetical protein YgdT; hypothetical protein 932147..932293 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156709 YP_003035147.1 CDS ECBD_0895 NC_012947.1 932646 933176 D hydrolyzes diadenosine polyphosphate; dinucleoside polyphosphate hydrolase 932646..933176 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156710 YP_003035148.1 CDS ECBD_0896 NC_012947.1 933189 935435 D member of a second PTS chain involved in nitrogen metabolism; PtsP phosphorylates NPr; fused phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain 933189..935435 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156711 YP_003035149.1 CDS ECBD_0897 NC_012947.1 935586 936461 D transfers the N-acyl diglyceride moiety to the prospective N-terminal cysteine in prolipoprotein; prolipoprotein diacylglyceryl transferase 935586..936461 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156712 YP_003035150.1 CDS thyA NC_012947.1 936468 937262 D ThyA; catalyzes formation of dTMP and 7,8-dihydrofolate from 5,10-methylenetetrahydrofolate and dUMP; involved in deoxyribonucleotide biosynthesis; there are 2 copies in some Bacilli, one of which appears to be phage-derived; thymidylate synthase 936468..937262 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156713 YP_003035151.1 CDS ECBD_0899 NC_012947.1 937447 937917 D KEGG: sdy:SDY_3043 hypothetical protein; hypothetical protein 937447..937917 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156714 YP_003035152.1 CDS ECBD_0900 NC_012947.1 937908 938471 D KEGG: ssn:SSON_2982 hypothetical protein; hypothetical protein 937908..938471 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156715 YP_003035153.1 CDS ECBD_0901 NC_012947.1 938468 938875 D KEGG: sbc:SbBS512_E3038 hypothetical protein; hypothetical protein 938468..938875 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156716 YP_003035154.1 CDS ECBD_0902 NC_012947.1 938860 939183 D KEGG: sbc:SbBS512_E3039 prepilin peptidase-dependent protein C; hypothetical protein 938860..939183 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156717 YP_003035155.1 CDS recC NC_012947.1 939196 942564 D catalyzes ATP-dependent exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides; component of the RecBCD (Exo V) helicase/nuclease complex that is essential for recombination; exonuclease V subunit gamma 939196..942564 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156718 YP_003035156.1 CDS ECBD_0904 NC_012947.1 942740 945628 D PFAM: peptidase M16 domain protein; KEGG: sfv:SFV_2899 protease III; peptidase M16 domain protein 942740..945628 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156719 YP_003035157.1 CDS recB NC_012947.1 945621 949163 D helicase/nuclease; with RecCD catalyzes the exonucleolytic cleavage of DNA; RecD has 5' to 3' helicase activity; exonuclease V subunit beta 945621..949163 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156720 YP_003035158.1 CDS recD NC_012947.1 949163 950989 D helicase/nuclease; with RecBC catalyzes the exonucleolytic cleavage of DNA; RecD has 5' to 3' helicase activity; exonuclease V subunit alpha 949163..950989 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156721 YP_003035159.1 CDS ECBD_0907 NC_012947.1 951051 952382 R catalyzes the formation of N-acetyl-L-glutamate from L-glutamate and acetyl-CoA in arginine biosynthesis; N-acetylglutamate synthase complement(951051..952382) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156722 YP_003035160.1 CDS ECBD_0908 NC_012947.1 952614 953867 D KEGG: sbc:SbBS512_E3045 N-acetylmuramoyl-L-alanine amidase AmiC; PFAM: cell wall hydrolase/autolysin; SMART: cell wall hydrolase/autolysin; N-acetylmuramoyl-L-alanine amidase 952614..953867 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156723 YP_003035161.1 CDS mltA NC_012947.1 954446 955543 D membrane-bound, lytic; catalyzes the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues; murein transglycosylase A 954446..955543 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156727 YP_003035162.1 CDS ECBD_0910 NC_012947.1 955782 956588 D PFAM: UBA/THIF-type NAD/FAD binding protein; KEGG: ssn:SSON_2969 enzyme; UBA/THIF-type NAD/FAD binding protein 955782..956588 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156728 YP_003035163.1 CDS ECBD_0911 NC_012947.1 956639 957082 R TIGRFAM: cysteine desulfurase, sulfur acceptor subunit CsdE; PFAM: Fe-S metabolism associated SufE; KEGG: ssn:SSON_2968 hypothetical protein; cysteine desulfurase, sulfur acceptor subunit CsdE complement(956639..957082) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156729 YP_003035164.1 CDS ECBD_0912 NC_012947.1 957082 958287 R PFAM: aminotransferase class V; KEGG: ssn:SSON_2967 cysteine sulfinate desulfinase; cysteine sulfinate desulfinase complement(957082..958287) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156730 YP_003035165.1 CDS ECBD_0913 NC_012947.1 958479 958706 D PFAM: protein of unknown function DUF903; KEGG: ssn:SSON_2966 hypothetical protein; hypothetical protein 958479..958706 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156731 YP_003035166.1 CDS ECBD_0914 NC_012947.1 959057 959974 D glycine cleavage system transcriptional activator; activates the gcvTHP operon in the presence of glycine and represses the operon in its absence; GcvA family transcriptional regulator 959057..959974 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156732 YP_003035167.1 CDS ECBD_0915 NC_012947.1 959993 960388 D PFAM: protein of unknown function DUF423; KEGG: sdy:SDY_3025 hypothetical protein; hypothetical protein 959993..960388 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156733 YP_003035168.1 CDS ECBD_0916 NC_012947.1 960381 961481 D RNA 2'-O-ribose methyltransferase 960381..961481 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156734 YP_003035169.1 CDS ECBD_0917 NC_012947.1 961525 962256 R regulates expression of genes involved in L-fucose utilization; FucR family transcriptional regulator complement(961525..962256) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156735 YP_003035170.1 CDS ECBD_0918 NC_012947.1 962314 962736 R PFAM: RbsD or FucU transport; KEGG: sdy:SDY_3022 protein of fucose operon; RbsD or FucU transport complement(962314..962736) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156736 YP_003035171.1 CDS ECBD_0919 NC_012947.1 962738 964156 R PFAM: carbohydrate kinase FGGY; KEGG: ssn:SSON_2960 L-fuculokinase; L-fuculokinase complement(962738..964156) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156737 YP_003035172.1 CDS fucI NC_012947.1 964265 966040 R catalyzes the conversion of the aldose L-fucose into the corresponding ketose L-fuculose; L-fucose isomerase complement(964265..966040) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156738 YP_003035173.1 CDS ECBD_0921 NC_012947.1 966510 968009 D PFAM: ABC transporter; SMART: ATPase AAA; KEGG: atc:AGR_C_5112 ABC transporter protein, ATP binding component; ABC transporter 966510..968009 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156739 YP_003035174.1 CDS ECBD_0922 NC_012947.1 968013 968996 D PFAM: inner-membrane translocator; KEGG: hap:HAPS_1726 ABC D-ribose transporter permease RbsC; inner-membrane translocator 968013..968996 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156740 YP_003035175.1 CDS ECBD_0923 NC_012947.1 969070 970020 D PFAM: periplasmic binding protein/LacI transcriptional regulator; KEGG: bcj:BCAM2280 solute-binding component of ABC transporter; periplasmic binding protein/LacI transcriptional regulator 969070..970020 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156741 YP_003035176.1 CDS ECBD_0924 NC_012947.1 970073 971653 D PFAM: carbohydrate kinase FGGY; KEGG: pmr:PMI2941 probable carbohydrate kinase; carbohydrate kinase FGGY 970073..971653 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156742 YP_003035177.1 CDS ECBD_0925 NC_012947.1 971718 972716 D PFAM: periplasmic binding protein/LacI transcriptional regulator; SMART: regulatory protein LacI; KEGG: pmr:PMI2934 LacI-family transcriptional regulator; LacI family transcriptional regulator 971718..972716 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156743 YP_003035178.1 CDS ECBD_0927 NC_012947.1 973344 973619 D PFAM: Insertion element protein; KEGG: hdu:HD1971 insertion element IS1 1/2/3/5/6 protein InsA; Insertion element protein 973344..973619 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156745 YP_003035179.1 CDS ECBD_0928 NC_012947.1 973538 974041 D PFAM: IS1 transposase; KEGG: dps:DP0860 insertion element IS1 1/5/6 protein (InsB); IS1 transposase 973538..974041 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156746 YP_003035180.1 CDS ECBD_0929 NC_012947.1 974301 975449 D TIGRFAM: lactaldehyde reductase; PFAM: iron-containing alcohol dehydrogenase; L-1,2-propanediol oxidoreductase 974301..975449 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156747 YP_003035181.1 CDS xni NC_012947.1 975504 976349 R has 3'-5' exonuclease activity that preferentially acts on ssDNA; also 3'-phosphodiesterase activity at sites with 3' incised apurinic/apyrimidinic sites; can remove 3' phosphoglycolate groups; exonuclease IX complement(975504..976349) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156748 YP_003035182.1 CDS ECBD_0931 NC_012947.1 976371 977738 R KEGG: ssn:SSON_2954 L-serine dehydratase; TIGRFAM: L-serine dehydratase; PFAM: serine dehydratase alpha chain; serine dehydratase beta chain; L-serine dehydratase complement(976371..977738) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156749 YP_003035183.1 CDS ECBD_0932 NC_012947.1 977796 979085 R TIGRFAM: serine transporter; KEGG: ssn:SSON_2953 probable serine transporter; serine transporter complement(977796..979085) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156750 YP_003035184.1 CDS ECBD_0933 NC_012947.1 979427 979558 R hypothetical protein complement(979427..979558) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156751 YP_003035185.1 CDS ECBD_0934 NC_012947.1 979642 981006 R PFAM: conserved hypothetical protein; KEGG: sbo:SBO_2676 hypothetical protein; hypothetical protein complement(979642..981006) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156752 YP_003035186.1 CDS queF NC_012947.1 981118 981966 R catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) in queuosine biosynthesis; 7-cyano-7-deazaguanine reductase complement(981118..981966) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156753 YP_003035187.1 CDS ECBD_0936 NC_012947.1 982034 982579 D SecY regulator that either chelates excess SecY or negatively regulates the SecYE translocase function; SecY interacting protein Syd 982034..982579 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156754 YP_003035188.1 CDS ECBD_0937 NC_012947.1 983201 983530 D PFAM: protein of unknown function DUF446; KEGG: ssn:SSON_2949 hypothetical protein; hypothetical protein 983201..983530 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156755 YP_003035189.1 CDS ECBD_0938 NC_012947.1 983530 984312 D catalyzes formation of pseudouridine at position 65 in tRNA-Ile1 and tRNA-Asp; tRNA pseudouridine synthase C 983530..984312 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156756 YP_003035190.1 CDS ECBD_0939 NC_012947.1 984330 984779 D An electron-transfer protein; flavodoxin binds one FMN molecule, which serves as a redox-active prosthetic group; flavodoxin 984330..984779 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156757 YP_003035191.1 CDS ECBD_0940 NC_012947.1 985214 986566 D TIGRFAM: d-galactonate transporter; PFAM: major facilitator superfamily MFS_1; d-galactonate transporter 985214..986566 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156758 YP_003035192.1 CDS ECBD_0941 NC_012947.1 986568 987908 D PFAM: mandelate racemase/muconate lactonizing protein; KEGG: ssn:SSON_2945 glucarate dehydratase; mandelate racemase/muconate lactonizing protein 986568..987908 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156759 YP_003035193.1 CDS ECBD_0942 NC_012947.1 987929 989269 D KEGG: sfx:S2994 glucarate dehydratase; TIGRFAM: glucarate dehydratase; PFAM: mandelate racemase/muconate lactonizing protein; glucarate dehydratase 987929..989269 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156760 YP_003035194.1 CDS ECBD_0943 NC_012947.1 989502 992258 R part of the two-component regulatory system with UvrY; involved in the regulation of carbon metabolism via the csrA/csrB regulatory system; hybrid sensory histidine kinase BarA complement(989502..992258) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156761 YP_003035195.1 CDS rumA NC_012947.1 992315 993616 D in Escherichia coli this enzyme catalyzes the SAM-dependent methylation of U1939 in the 23S ribomal RNA; binds an iron-sulfur cluster [4Fe-4S]; 23S rRNA 5-methyluridine methyltransferase 992315..993616 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156762 YP_003035196.1 CDS relA NC_012947.1 993664 995898 D (p)ppGpp synthetase; catalyzes the formation of pppGpp and ppGpp from ATP and GTP or GDP; GDP/GTP pyrophosphokinase 993664..995898 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156763 YP_003035197.1 CDS ECBD_0946 NC_012947.1 995976 996224 D part of the MazF-MazE toxin-antitoxin system; conteracts MazF; antitoxin MazE 995976..996224 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156764 YP_003035198.1 CDS ECBD_0947 NC_012947.1 996224 996559 D MazF; endoribonuclease; toxin of the ChpA-ChpR toxin-antitoxin system; toxin ChpA 996224..996559 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156765 YP_003035199.1 CDS mazG NC_012947.1 996630 997421 D functions in degradation of stringent response intracellular messenger ppGpp; in Escherichia coli this gene is co-transcribed with the toxin/antitoxin genes mazEF; activity of MazG is inhibited by MazEF in vitro; ppGpp inhibits mazEF expression; MazG thus works in limiting the toxic activity of the MazF toxin induced during starvation; MazG also interacts with the GTPase protein Era; nucleoside triphosphate pyrophosphohydrolase 996630..997421 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156766 YP_003035200.1 CDS pyrG NC_012947.1 997649 999286 D CTP synthase; cytidine triphosphate synthetase; catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; in Escherichia coli this enzyme forms a homotetramer; CTP synthetase 997649..999286 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156767 YP_003035201.1 CDS eno NC_012947.1 999374 1000672 D enolase; catalyzes the formation of phosphoenolpyruvate from 2-phospho-D-glycerate in glycolysis; phosphopyruvate hydratase 999374..1000672 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156768 YP_003035202.1 CDS ECBD_0951 NC_012947.1 1000732 1001604 R PFAM: protein of unknown function DUF477; KEGG: sfv:SFV_2677 hypothetical protein; hypothetical protein complement(1000732..1001604) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156769 YP_003035203.1 CDS ECBD_0952 NC_012947.1 1001618 1001758 R hypothetical protein complement(1001618..1001758) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156770 YP_003035204.1 CDS ECBD_0953 NC_012947.1 1001897 1002568 D KEGG: sdy:SDY_2981 hypothetical protein; hypothetical protein 1001897..1002568 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156771 YP_003035205.1 CDS ECBD_0954 NC_012947.1 1004369 1005847 R PFAM: carbohydrate kinase FGGY; KEGG: sdy:SDY_2978 kinase; carbohydrate kinase FGGY complement(1004369..1005847) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156772 YP_003035206.1 CDS ECBD_0955 NC_012947.1 1005874 1007151 R PFAM: major facilitator superfamily MFS_1; KEGG: ssn:SSON_2927 transporter; major facilitator superfamily MFS_1 complement(1005874..1007151) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156773 YP_003035207.1 CDS ECBD_0956 NC_012947.1 1007470 1008255 D PFAM: short-chain dehydrogenase/reductase SDR; KEGG: elf:LF82_3153 uncharacterized oxidoreductase YgcW; short-chain dehydrogenase/reductase SDR 1007470..1008255 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156774 YP_003035208.1 CDS ECBD_0957 NC_012947.1 1008325 1009779 D PFAM: FAD linked oxidase; Alkylglycerone-phosphate synthase 1008325..1009779 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156775 YP_003035209.1 CDS ECBD_0958 NC_012947.1 1009801 1011210 D PFAM: major facilitator superfamily MFS_1; general substrate transporter; KEGG: ssn:SSON_2924 transporter; major facilitator superfamily MFS_1 1009801..1011210 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156776 YP_003035210.1 CDS ECBD_0959 NC_012947.1 1011188 1011967 D PFAM: electron transfer flavoprotein alpha/beta-subunit; KEGG: ssn:SSON_2923 transporter; electron transfer flavoprotein alpha/beta-subunit 1011188..1011967 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156777 YP_003035211.1 CDS ECBD_0960 NC_012947.1 1011964 1012824 D PFAM: electron transfer flavoprotein subunit alpha; KEGG: ssn:SSON_2922 flavoprotein; electron transfer flavoprotein subunit alpha 1011964..1012824 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156778 YP_003035212.1 CDS ECBD_0961 NC_012947.1 1012972 1013547 R PFAM: glycerol-3-phosphate responsive antiterminator; KEGG: elf:LF82_3148 uncharacterized protein YgcP; glycerol-3-phosphate responsive antiterminator, GlpP complement(1012972..1013547) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156779 YP_003035213.1 CDS ECBD_0962 NC_012947.1 1013564 1013824 R KEGG: sdy:SDY_2969 hypothetical protein; hypothetical protein complement(1013564..1013824) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158993 YP_003035214.1 CDS ECBD_0963 NC_012947.1 1013815 1015086 R PFAM: FAD dependent oxidoreductase; monooxygenase FAD-binding; HI0933 family protein; KEGG: ssn:SSON_2919 hypothetical protein; FAD dependent oxidoreductase complement(1013815..1015086) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156016 YP_003035215.1 CDS ECBD_0964 NC_012947.1 1015164 1015529 R TIGRFAM: 6-pyruvoyl tetrahydropterin synthase; PFAM: 6-pyruvoyl tetrahydropterin synthase and hypothetical protein; KEGG: ssn:SSON_2918 6-pyruvoyl tetrahydrobiopterin synthase; 6-pyruvoyl tetrahydropterin synthase complement(1015164..1015529) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156017 YP_003035216.1 CDS cysJ NC_012947.1 1015845 1017644 D catalyzes the reduction of sulfite to sulfide in the biosynthesis of L-cysteine from sulfate; a flavoprotein with flavin reductase activity; sulfite reductase subunit alpha 1015845..1017644 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156018 YP_003035217.1 CDS ECBD_0966 NC_012947.1 1017644 1019356 D hemoprotein; NADPH dependent; with the subunit alpha (a flavoprotein) catalyzes the reduction of sulfite to sulfide; sulfite reductase subunit beta 1017644..1019356 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156019 YP_003035218.1 CDS ECBD_0967 NC_012947.1 1019430 1020164 D catalyzes the reduction of 3'-phosphoadenylyl sulfate into sulfite; phosphoadenosine phosphosulfate reductase 1019430..1020164 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156020 YP_003035219.1 CDS ECBD_0968 NC_012947.1 1020429 1020581 D PFAM: Hok/gef cell toxic protein; KEGG: sbc:SbBS512_E3114 hok/gef family protein; Hok/gef cell toxic protein 1020429..1020581 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156021 YP_003035220.1 CDS ECBD_0969 NC_012947.1 1020611 1020751 R hypothetical protein complement(1020611..1020751) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156022 YP_003035221.1 CDS ECBD_0970 NC_012947.1 1020774 1021886 R PFAM: transposase IS4 family protein; transposase IS4 family protein complement(1020774..1021886) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156023 YP_003035222.1 CDS ECBD_0971 NC_012947.1 1022318 1023355 R catalyzes the sequential removal of 2 amino-terminal arginines from alkaline phosphatase isozyme 1 to form isozymes 2 and 3; alkaline phosphatase isozyme conversion aminopeptidase complement(1022318..1023355) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156024 YP_003035223.1 CDS ECBD_0972 NC_012947.1 1023607 1024515 D with CysN catalyzes the formation of adenylylsulfate from sulfate and ATP; sulfate adenylyltransferase subunit 2 1023607..1024515 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156025 YP_003035224.1 CDS cysN NC_012947.1 1024517 1025944 D may be GTPase that regulates ATP sulfurylase activity that is involved in converting ATP and sulfate to diphosphate and adenylylsulfate; in Escherichia coli this enzyme functions in cysteine biosynthesis in the first step; forms a heterodimer with CysD; part of the GTP-binding elongation factor family CysN/NodQ; sulfate adenylyltransferase subunit 1 1024517..1025944 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156026 YP_003035225.1 CDS ECBD_0974 NC_012947.1 1025944 1026549 D converts ATP and adenylyl sulfate to ADP and 3'-phosphoadenylyl sulfate; in Escherichia coli this enzyme functions in cysteine biosynthesis; adenylylsulfate kinase 1025944..1026549 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156027 YP_003035226.1 CDS ECBD_0975 NC_012947.1 1026599 1026922 D KEGG: sdy:SDY_2948 hypothetical protein; hypothetical protein 1026599..1026922 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156028 YP_003035227.1 CDS ftsB NC_012947.1 1027116 1027427 D forms a complex with FtsL and FtsQ; colocalizes to the septal region of the dividing cell; membrane protein; cell division protein FtsB 1027116..1027427 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156029 YP_003035228.1 CDS ispD NC_012947.1 1027446 1028156 D 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate; involved in isoprenoid and isopentenyl-PP biosynthesis; forms homodimers; 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1027446..1028156 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156030 YP_003035229.1 CDS ispF NC_012947.1 1028156 1028635 D catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase 1028156..1028635 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156031 YP_003035230.1 CDS truD NC_012947.1 1028632 1029681 D catalyzes the modification of U13 in tRNA(Glu); tRNA pseudouridine synthase D 1028632..1029681 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156032 YP_003035231.1 CDS surE NC_012947.1 1029662 1030423 D catalyzes the conversion of a phosphate monoester to an alcohol and a phosphate; stationary phase survival protein SurE 1029662..1030423 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156033 YP_003035232.1 CDS pcm NC_012947.1 1030417 1031043 D catalyzes the methyl esterification of L-isoaspartyl residues that are formed in damaged proteins; protein-L-isoaspartate O-methyltransferase 1030417..1031043 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156034 YP_003035233.1 CDS nlpD NC_012947.1 1031183 1032322 D outer membrane lipoprotein involved in stationary-phase cell survival; similar to LppB virulence determinant from Haemophilus somnus; lipoprotein NlpD 1031183..1032322 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156035 YP_003035234.1 CDS ECBD_0983 NC_012947.1 1032385 1033377 D sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; this sigma factor controls a regulon of genes required for protection against external stresses; RNA polymerase sigma factor RpoS 1032385..1033377 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156036 YP_003035235.1 CDS ECBD_0984 NC_012947.1 1033471 1034835 R member of the Gnt family of gluconate transporters; not involved in gluconate transport; unknown function; inner membrane permease YgbN complement(1033471..1034835) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156037 YP_003035236.1 CDS ECBD_0985 NC_012947.1 1034924 1035700 R PFAM: xylose isomerase; KEGG: sbc:SbBS512_E3135 AP endonuclease, family 2; hypothetical protein complement(1034924..1035700) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156038 YP_003035237.1 CDS ECBD_0986 NC_012947.1 1035705 1036343 R PFAM: class II aldolase/adducin family protein; KEGG: ssn:SSON_2886 aldolase; aldolase complement(1035705..1036343) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156039 YP_003035238.1 CDS ECBD_0987 NC_012947.1 1036340 1037602 R PFAM: type III effector Hrp-dependent outers; KEGG: ssn:SSON_2885 hypothetical protein; Hrp-dependent type III effector protein complement(1036340..1037602) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156040 YP_003035239.1 CDS ECBD_0988 NC_012947.1 1037599 1038507 R PFAM: 6-phosphogluconate dehydrogenase NAD-binding; KEGG: sbc:SbBS512_E3138 NAD binding domain of 6-phosphogluconate dehydrogenase; 2-hydroxy-3-oxopropionate reductase complement(1037599..1038507) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156041 YP_003035240.1 CDS ECBD_0989 NC_012947.1 1038703 1039470 D PFAM: regulatory protein DeoR; Helix-turn-helix type 11 domain protein; SMART: regulatory protein DeoR; KEGG: sbo:SBO_2785 DeoR-type transcriptional regulator; DeoR family transcriptional regulator 1038703..1039470 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156042 YP_003035241.1 CDS ECBD_0990 NC_012947.1 1039521 1040177 R PFAM: metallophosphoesterase; KEGG: sbo:SBO_2786 serine/threonine-specific protein phosphatase 2; serine/threonine-specific protein phosphatase 2 complement(1039521..1040177) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156043 YP_003035242.1 CDS ECBD_0991 NC_012947.1 1040283 1042844 R This protein performs the mismatch recognition step during the DNA repair process; DNA mismatch repair protein MutS complement(1040283..1042844) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156044 YP_003035243.1 CDS ECBD_0992 NC_012947.1 1042920 1043054 D hypothetical protein 1042920..1043054 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156045 YP_003035244.1 CDS ECBD_0993 NC_012947.1 1043131 1043484 D KEGG: ssn:SSON_2879 hypothetical protein; hypothetical protein 1043131..1043484 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156046 YP_003035245.1 CDS ECBD_0994 NC_012947.1 1043521 1045599 R PFAM: sigma-54 factor interaction domain-containing protein; helix-turn-helix Fis-type; GAF domain protein; ATPase associated with various cellular activities AAA_5; SMART: ATPase AAA; GAF domain protein; Fis family transcriptional regulator complement(1043521..1045599) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156047 YP_003035246.1 CDS ECBD_0995 NC_012947.1 1045673 1046683 R TIGRFAM: hydrogenase expression/formation protein HypE; PFAM: AIR synthase related protein domain protein; AIR synthase related protein; KEGG: sbc:SbBS512_E3147 hydrogenase expression/formation protein HypE; hydrogenase expression/formation protein HypE complement(1045673..1046683) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156048 YP_003035247.1 CDS ECBD_0996 NC_012947.1 1046680 1047801 R TIGRFAM: hydrogenase expression/formation protein HypD; PFAM: hydrogenase formation HypD protein; KEGG: sbc:SbBS512_E3148 hydrogenase expression/formation protein HypD; hydrogenase expression/formation protein HypD complement(1046680..1047801) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156049 YP_003035248.1 CDS ECBD_0997 NC_012947.1 1047801 1048073 R HypC; accessory protein necessary for maturation of the hydrogenase isoforms 1, 2 and 3; forms a complex with HypD, HypE, and HypF proteins, which is the site of ligand biosynthesis and attachment to the iron atom of the NiFe site in the hydrogenase; hydrogenase assembly chaperone complement(1047801..1048073) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156050 YP_003035249.1 CDS ECBD_0998 NC_012947.1 1048064 1048936 R GTP hydrolase involved in nickel liganding into hydrogenases; hydrogenase nickel incorporation protein HypB complement(1048064..1048936) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156051 YP_003035250.1 CDS hypA NC_012947.1 1048940 1049290 R plays a role in hydrogenase nickel cofactor insertion; hydrogenase nickel incorporation protein complement(1048940..1049290) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156052 YP_003035251.1 CDS ECBD_1000 NC_012947.1 1049502 1049963 D regulates several genes involved in the formate hydrogenlyase system; seems to prevent binding of FhlA transcriptional activator to the activator sequence of hyc operon; formate hydrogenlyase regulatory protein HycA 1049502..1049963 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156053 YP_003035252.1 CDS ECBD_1001 NC_012947.1 1050088 1050699 D PFAM: 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: sfv:SFV_2779 small subunit of hydrogenase-3, iron-sulfur protein (part of formate hydrogenlyase (FHL) complex); 4Fe-4S ferredoxin iron-sulfur binding domain protein 1050088..1050699 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156054 YP_003035253.1 CDS ECBD_1002 NC_012947.1 1050696 1052522 D catalyzes the oxidation of formate to carbon dioxide and molecular hydrogen; formate hydrogenlyase comprises of a formate dehydrogenase, unidentified electron carriers and a hydrogenase (subunit 3); formate hydrogenlyase subunit 3 1050696..1052522 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156055 YP_003035254.1 CDS ECBD_1003 NC_012947.1 1052525 1053448 D PFAM: respiratory-chain NADH dehydrogenase subunit 1; KEGG: ssn:SSON_2869 membrane-spanning protein of hydrogenase 3 (part of FHL complex); respiratory-chain NADH dehydrogenase subunit 1 1052525..1053448 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156056 YP_003035255.1 CDS ECBD_1004 NC_012947.1 1053466 1055175 D PFAM: NADH dehydrogenase (ubiquinone) 30 kDa subunit; KEGG: sbo:SBO_2797 large subunit of hydrogenase 3; NADH dehydrogenase (ubiquinone) 30 kDa subunit 1053466..1055175 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156057 YP_003035256.1 CDS ECBD_1005 NC_012947.1 1055185 1055727 D electron transfer protein for hydrogenase-3; the formate hydrogenlyase complex comprises of a formate dehydrogenase, unidentified electron carriers and hydrogenase-3; in this non-energy conserving pathway, molecular hydrogen and carbodioxide are released from formate; formate hydrogenlyase complex iron-sulfur subunit 1055185..1055727 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156058 YP_003035257.1 CDS ECBD_1006 NC_012947.1 1055727 1056494 D PFAM: NADH ubiquinone oxidoreductase 20 kDa subunit; KEGG: elf:LF82_1069 formate hydrogenlyase subunit 7; NADH ubiquinone oxidoreductase 20 kDa subunit 1055727..1056494 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156059 YP_003035258.1 CDS ECBD_1007 NC_012947.1 1056491 1056901 D PFAM: formate hydrogenlyase maturation HycH; KEGG: sbc:SbBS512_E3158 formate hydrogenlyase maturation protein; formate hydrogenlyase maturation HycH 1056491..1056901 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156060 YP_003035259.1 CDS hycI NC_012947.1 1056894 1057364 D involved in the C-terminal processing of the large subunit of hydrogenase 3 HycE; hydrogenase 3 maturation protease 1056894..1057364 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156061 YP_003035260.1 CDS ECBD_1009 NC_012947.1 1057523 1058947 R PFAM: glycoside hydrolase family 1; KEGG: ssn:SSON_2860 cryptic 6-phospho-beta-glucosidase; cryptic 6-phospho-beta-glucosidase complement(1057523..1058947) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156062 YP_003035261.1 CDS ECBD_1010 NC_012947.1 1058956 1060413 R phosphoenolpyruvate-dependent sugar phosphotransferase system; catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane; IIB is phosphorylated by IIA and then transfers the phosphoryl group to the sugar; IIC forms the translocation channel; PTS system cellobiose/arbutin/salicin-specific transporter subunits IIBC complement(1058956..1060413) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156063 YP_003035262.1 CDS ECBD_1011 NC_012947.1 1060670 1061683 D PFAM: regulatory protein LacI; periplasmic binding protein/LacI transcriptional regulator; SMART: regulatory protein LacI; KEGG: ssn:SSON_2858 ascBF operon repressor; LacI family transcriptional regulator 1060670..1061683 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156064 YP_003035263.1 CDS ECBD_1012 NC_012947.1 1061832 1062359 D involved in electron transport from formate to hydrogen; electron transporter HydN 1061832..1062359 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156065 YP_003035264.1 CDS ECBD_1013 NC_012947.1 1062512 1064764 D TIGRFAM: [NiFe] hydrogenase maturation protein HypF; PFAM: SUA5/yciO/yrdC domain; acylphosphatase; zinc finger HypF domain protein; KEGG: ssn:SSON_2856 transcriptional regulatory protein; (NiFe) hydrogenase maturation protein HypF 1062512..1064764 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156066 YP_003035265.1 CDS ECBD_1014 NC_012947.1 1064892 1066025 R catalyzes the reduction of the rubredoxin moiety of nitric oxide reductase; nitric oxide reductase complement(1064892..1066025) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156067 YP_003035266.1 CDS ECBD_1015 NC_012947.1 1066022 1067461 R detoxifies nitric oxide using NADH; anaerobic nitric oxide reductase flavorubredoxin complement(1066022..1067461) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156068 YP_003035267.1 CDS ECBD_1016 NC_012947.1 1067648 1069162 D Required for the expression of anaerobic nitric oxide (NO) reductase; acts as a transcriptional activator for the norVW operon; anaerobic nitric oxide reductase transcription regulator 1067648..1069162 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156069 YP_003035268.1 CDS gutQ NC_012947.1 1069159 1070124 R catalyzes the conversion of D-arabinose 5-phosphate to D-ribulose-5-phosphate; D-arabinose 5-phosphate isomerase complement(1069159..1070124) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156070 YP_003035269.1 CDS srlR NC_012947.1 1070117 1070890 R regulates genes involved in glucitol utilization; DNA-binding transcriptional repressor SrlR complement(1070117..1070890) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156071 YP_003035270.1 CDS ECBD_1019 NC_012947.1 1070957 1071316 R regulator for glucitol utilization; DNA-binding transcriptional activator GutM complement(1070957..1071316) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156072 YP_003035271.1 CDS ECBD_1020 NC_012947.1 1071422 1072201 R catalyzes the conversion of sorbitol 6-phosphate into fructose 6-phosphate; sorbitol-6-phosphate dehydrogenase complement(1071422..1072201) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156073 YP_003035272.1 CDS ECBD_1021 NC_012947.1 1072205 1072576 R phosphoenolpyruvate-dependent sugar phosphotransferase system; catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane; IIB is phosphorylated by IIA and then transfers the phosphoryl group to the sugar; IIC forms the translocation channel; PTS system glucitol/sorbitol-specific transporter subunit IIA complement(1072205..1072576) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156074 YP_003035273.1 CDS ECBD_1022 NC_012947.1 1072587 1073546 R KEGG: sbc:SbBS512_E3174 PTS system, glucitol/sorbitol-specific, IIB component; TIGRFAM: PTS system, glucitol/sorbitol-specific, IIBC subunit; PFAM: Sorbitol phosphotransferase protein II domain protein; PTS system, glucitol/sorbitol-specific, IIBC subunit complement(1072587..1073546) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156075 YP_003035274.1 CDS ECBD_1023 NC_012947.1 1073543 1074106 R TIGRFAM: PTS system, glucitol/sorbitol-specific, IIC subunit; PFAM: PTS system protein II sorbitol-specific factor; KEGG: sdy:SDY_2898 PTS system, glucitol/sorbitol-specific IIC component; PTS system, glucitol/sorbitol-specific, IIC subunit complement(1073543..1074106) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156076 YP_003035275.1 CDS ECBD_1024 NC_012947.1 1074362 1075447 D membrane-bound lytic murein transglycosylase B; catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine in peptidoglycan; murein hydrolase B 1074362..1075447 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156077 YP_003035276.1 CDS ECBD_1025 NC_012947.1 1075592 1076089 D PFAM: damage inducible protein CinA; KEGG: ssn:SSON_2844 competence damage-inducible protein A; competence damage-inducible protein A 1075592..1076089 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156078 YP_003035277.1 CDS recA NC_012947.1 1076169 1077230 D catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs; recombinase A 1076169..1077230 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156079 YP_003035278.1 CDS recX NC_012947.1 1077299 1077799 D binds RecA and inhibits RecA-mediated DNA strand exchange and ATP hydrolysis and coprotease activities; recombination regulator RecX 1077299..1077799 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156080 YP_003035279.1 CDS alaS NC_012947.1 1077927 1080557 D Catalyzes a two-step reaction, first charging an alanyl molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; alanyl-tRNA synthetase 1077927..1080557 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156081 YP_003035280.1 CDS ECBD_1029 NC_012947.1 1080792 1080977 D affects carbohydrate metabolism; has regulatory role in many processes; carbon storage regulator 1080792..1080977 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156082 YP_003035281.1 CDS ECBD_1030 NC_012947.1 1082435 1083001 D YqaB; catalyzes the dephosphorylation of fructose 1-phosphate, 6-phosphogluconate and p-nitrophenyl phosphate (pNPP); presents beta-phosphoglucomutase activity at a lower extent; fructose-1-phosphatase 1082435..1083001 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156088 YP_003035282.1 CDS ECBD_1031 NC_012947.1 1082998 1083426 D KEGG: sfv:SFV_2815 hypothetical protein; hypothetical protein 1082998..1083426 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156089 YP_003035283.1 CDS ECBD_1032 NC_012947.1 1083499 1085055 D involved in the first step of glutathione biosynthesis; glutamate--cysteine ligase 1083499..1085055 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156090 YP_003035284.1 CDS ECBD_1033 NC_012947.1 1085205 1085720 D catalyzes the hydrolysis of S-ribosylhomocysteine to homocysteine and autoinducer-2; S-ribosylhomocysteinase 1085205..1085720 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156091 YP_003035285.1 CDS ECBD_1034 NC_012947.1 1085784 1087322 R TIGRFAM: drug resistance transporter EmrB/QacA subfamily; PFAM: major facilitator superfamily MFS_1; drug resistance transporter EmrB/QacA subfamily complement(1085784..1087322) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156092 YP_003035286.1 CDS ECBD_1035 NC_012947.1 1087339 1088511 R TIGRFAM: efflux pump membrane protein; PFAM: secretion protein HlyD family protein; KEGG: ssn:SSON_2829 multidrug resistance secretion protein; efflux pump membrane protein complement(1087339..1088511) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156093 YP_003035287.1 CDS ECBD_1036 NC_012947.1 1088638 1089168 R DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux; negative regulator of the multidrug operon emrAB; transcriptional repressor MprA complement(1088638..1089168) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156094 YP_003035288.1 CDS ECBD_1037 NC_012947.1 1089259 1089594 R KEGG: sbc:SbBS512_E3193 hypothetical protein; hypothetical protein complement(1089259..1089594) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156095 YP_003035289.1 CDS ECBD_1038 NC_012947.1 1089584 1090321 R PFAM: AzlC family protein; KEGG: ssn:SSON_2826 hypothetical protein; AzlC family protein complement(1089584..1090321) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156096 YP_003035290.1 CDS ECBD_1039 NC_012947.1 1090445 1091629 R PFAM: major facilitator superfamily MFS_1; KEGG: sbo:SBO_2836 permease; major facilitator superfamily MFS_1 complement(1090445..1091629) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159482 YP_003035291.1 CDS proX NC_012947.1 1091921 1092913 R with ProVW, part of the high-affinity transporter for the osmoprotectant glycine betaine; glycine betaine transporter periplasmic subunit complement(1091921..1092913) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159483 YP_003035292.1 CDS ECBD_1041 NC_012947.1 1092971 1094035 R with ProVX is involved in the high-affinity uptake of glycine betaine; glycine betaine transporter membrane protein complement(1092971..1094035) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159484 YP_003035293.1 CDS ECBD_1042 NC_012947.1 1094028 1095230 R with ProWX is involved in the high-affinity uptake of glycine betaine; glycine betaine transporter ATP-binding subunit complement(1094028..1095230) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159485 YP_003035294.1 CDS nrdF NC_012947.1 1095584 1096543 R B2 or R2 protein; type 1b enzyme; catalyzes the rate-limiting step in dNTP synthesis; converts nucleotides to deoxynucleotides; forms a homodimer and then a multimeric complex with NrdE; ribonucleotide-diphosphate reductase subunit beta complement(1095584..1096543) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159486 YP_003035295.1 CDS ECBD_1044 NC_012947.1 1096553 1098697 R Catalyzes the rate-limiting step in dNTP synthesis; ribonucleotide-diphosphate reductase subunit alpha complement(1096553..1098697) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159487 YP_003035296.1 CDS nrdI NC_012947.1 1098670 1099080 R in Salmonella NrdI has a stimulatory effect on the ribonucleotide reductase activity of NrdH with NrdEF; ribonucleotide reductase stimulatory protein complement(1098670..1099080) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159488 YP_003035297.1 CDS ECBD_1046 NC_012947.1 1099077 1099322 R TIGRFAM: glutaredoxin-like protein NrdH; PFAM: glutaredoxin; KEGG: ssn:SSON_2818 glutaredoxin-like protein; glutaredoxin-like protein complement(1099077..1099322) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159489 YP_003035298.1 CDS ECBD_1047 NC_012947.1 1099570 1099899 R PFAM: protein of unknown function DUF883 ElaB; KEGG: sdy:SDY_2863 hypothetical protein; hypothetical protein complement(1099570..1099899) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159490 YP_003035299.1 CDS ECBD_1048 NC_012947.1 1100051 1100395 D KEGG: sbc:SbBS512_E3210 hypothetical protein; hypothetical protein 1100051..1100395 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159491 YP_003035300.1 CDS ECBD_1049 NC_012947.1 1100432 1100881 R PFAM: protein of unknown function DUF1144; KEGG: sbc:SbBS512_E3211 hypothetical protein; hypothetical protein complement(1100432..1100881) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159492 YP_003035301.1 CDS ECBD_1050 NC_012947.1 1101549 1101953 D PFAM: histone family protein nucleoid-structuring protein H-NS; SMART: histone family protein nucleoid-structuring protein H-NS; KEGG: sfx:S2883 DNA binding protein, nucleoid-associated; DNA binding protein, nucleoid-associated 1101549..1101953 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159493 YP_003035302.1 CDS ECBD_1051 NC_012947.1 1102000 1102524 R SMART: rhodanese; KEGG: sbc:SbBS512_E3213 inner membrane protein; rhodanese complement(1102000..1102524) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159494 YP_003035303.1 CDS ECBD_1052 NC_012947.1 1102534 1102833 R PFAM: regulatory protein ArsR; SMART: regulatory protein ArsR; KEGG: sbo:SBO_2852 hypothetical protein; ArsR family transcriptional regulator complement(1102534..1102833) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159495 YP_003035304.1 CDS ECBD_1053 NC_012947.1 1103016 1103174 D PFAM: protein of unknown function UPF0057; KEGG: sdy:SDY_2856 hypothetical protein; hypothetical protein 1103016..1103174 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159496 YP_003035305.1 CDS ECBD_1054 NC_012947.1 1103258 1103707 D PFAM: peptidoglycan-binding LysM; transport-associated; SMART: peptidoglycan-binding LysM; transport-associated and nodulation region; KEGG: ssn:SSON_2809 hypothetical protein; LysM domain/BON superfamily protein 1103258..1103707 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159497 YP_003035306.1 CDS ECBD_1055 NC_012947.1 1103708 1104370 R regulator of gab gene expression; DNA-binding transcriptional regulator CsiR complement(1103708..1104370) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159498 YP_003035307.1 CDS ECBD_1056 NC_012947.1 1104391 1105791 R TIGRFAM: GABA permease; PFAM: amino acid permease-associated region; KEGG: sdy:SDY_2853 gamma-aminobutyrate transporter; gamma-aminobutyrate transporter complement(1104391..1105791) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159499 YP_003035308.1 CDS ECBD_1057 NC_012947.1 1106102 1107382 R catalyzes the formation of succinate semialdehyde and glutamate from 4-aminobutanoate and 2-oxoglutarate; 4-aminobutyrate aminotransferase complement(1106102..1107382) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159500 YP_003035309.1 CDS gabD NC_012947.1 1107396 1108844 R catalyzes the formation of succinate from succinate semialdehyde; NADP dependent; succinate-semialdehyde dehydrogenase I complement(1107396..1108844) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159501 YP_003035310.1 CDS ECBD_1059 NC_012947.1 1108867 1110135 R catalyzed the formation of 2-ketoglutarate from 2-hydroxyglutarate; hydroxyglutarate oxidase complement(1108867..1110135) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159502 YP_003035311.1 CDS ECBD_1060 NC_012947.1 1110154 1111131 R in Escherichia coli this gene is induced by carbon starvation and depends on sigma S and cAMP-CRP; the structure of the Gab protein shows it is a member of non-heme iron (II)-dependent oxygenase superfamily which includes clavamini acid synthases; forms homotetramers in solution; hypothetical protein complement(1110154..1111131) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159503 YP_003035312.1 CDS ECBD_1061 NC_012947.1 1111437 1111940 R PFAM: IS1 transposase; IS1 transposase complement(1111437..1111940) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159504 YP_003035313.1 CDS ECBD_1062 NC_012947.1 1111859 1112134 R PFAM: Insertion element protein; KEGG: hdu:HD1971 insertion element IS1 1/2/3/5/6 protein InsA; Insertion element protein complement(1111859..1112134) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159505 YP_003035314.1 CDS ECBD_1063 NC_012947.1 1112533 1113429 R PFAM: RepB plasmid partition; SMART: ParB domain protein nuclease; KEGG: ent:Ent638_2890 RepB plasmid partition; RepB plasmid partition complement(1112533..1113429) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159506 YP_003035315.1 CDS ECBD_1064 NC_012947.1 1113426 1114322 R PFAM: ParB domain protein nuclease; SMART: ParB domain protein nuclease; KEGG: ent:Ent638_2889 nuclease; ParB domain protein nuclease complement(1113426..1114322) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159507 YP_003035316.1 CDS ECBD_1065 NC_012947.1 1114312 1115868 R PFAM: Recombinase; Resolvase domain; KEGG: ent:Ent638_2888 recombinase; Recombinase complement(1114312..1115868) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159508 YP_003035317.1 CDS ECBD_1066 NC_012947.1 1116151 1117380 R PFAM: integrase; KEGG: cko:CKO_03944 hypothetical protein; integrase complement(1116151..1117380) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159509 YP_003035318.1 CDS smpB NC_012947.1 1118124 1118606 R binds to ssrA RNA (tmRNA) and is required for its successful binding to ribosomes; also appears to function in the trans-translation step by promoting accommodation of tmRNA into the ribosomal A site; SmpB protects the tmRNA from RNase R degradation in Caulobacter crescentus; both the tmRNA and SmpB are regulated in cell cycle-dependent manner; functions in release of stalled ribosomes from damaged mRNAs and targeting proteins for degradation; SsrA-binding protein complement(1118124..1118606) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159511 YP_003035319.1 CDS ECBD_1068 NC_012947.1 1118738 1119214 D PFAM: cyclase/dehydrase; KEGG: efe:EFER_0454 hypothetical protein; hypothetical protein 1118738..1119214 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159512 YP_003035320.1 CDS ECBD_1069 NC_012947.1 1119204 1119494 D PFAM: protein of unknown function UPF0125; KEGG: sbc:SbBS512_E3007 RnfH family protein; hypothetical protein 1119204..1119494 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159513 YP_003035321.1 CDS ECBD_1070 NC_012947.1 1119556 1119897 R PFAM: SmpA/OmlA domain protein; KEGG: sdy:SDY_2790 hypothetical protein; hypothetical protein complement(1119556..1119897) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159514 YP_003035322.1 CDS ECBD_1071 NC_012947.1 1120046 1121707 R TIGRFAM: DNA repair protein RecN; PFAM: SMC domain protein; KEGG: ssn:SSON_2772 recombination and repair protein; recombination and repair protein complement(1120046..1121707) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159515 YP_003035323.1 CDS ppnK NC_012947.1 1121793 1122671 R catalyzes the phosphorylation of NAD to NADP; inorganic polyphosphate/ATP-NAD kinase complement(1121793..1122671) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159516 YP_003035324.1 CDS ECBD_1073 NC_012947.1 1122794 1123387 D with DnaK and DnaJ acts in response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins; may act as a thermosensor; heat shock protein GrpE 1122794..1123387 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159517 YP_003035325.1 CDS ECBD_1074 NC_012947.1 1123442 1124704 R PFAM: protein of unknown function DUF21; CBS domain containing protein; transporter-associated region; KEGG: sbc:SbBS512_E3001 CBS/transporter associated domain protein; hypothetical protein complement(1123442..1124704) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159518 YP_003035326.1 CDS ECBD_1075 NC_012947.1 1124749 1125615 R PFAM: cytochrome C assembly protein; KEGG: sdy:SDY_2785 hypothetical protein; cytochrome C assembly protein complement(1124749..1125615) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159519 YP_003035327.1 CDS ECBD_1076 NC_012947.1 1125707 1127068 D with 4.5S RNA forms a signal recognition particle involved in targeting and integration of inner membrane proteins; signal recognition particle protein 1125707..1127068 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159520 YP_003035328.1 CDS rpsP NC_012947.1 1127205 1127453 D binds to lower part of 30S body where it stabilizes two domains; required for efficient assembly of 30S; in Escherichia coli this protein has nuclease activity; 30S ribosomal protein S16 1127205..1127453 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159521 YP_003035329.1 CDS rimM NC_012947.1 1127472 1128020 D Essential for efficient processing of 16S rRNA; 16S rRNA-processing protein RimM 1127472..1128020 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159522 YP_003035330.1 CDS trmD NC_012947.1 1128051 1128818 D methylates guanosine-37 in various tRNAs; uses S-adenosyl-L-methionine to transfer methyl group to tRNA; tRNA (guanine-N(1)-)-methyltransferase 1128051..1128818 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159523 YP_003035331.1 CDS rplS NC_012947.1 1128860 1129207 D this protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site; 50S ribosomal protein L19 1128860..1129207 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159524 YP_003035332.1 CDS ECBD_1081 NC_012947.1 1129283 1129765 R PFAM: OmpA/MotB domain protein; KEGG: ssn:SSON_2762 predicted outer membrane lipoprotein; outer membrane lipoprotein complement(1129283..1129765) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159525 YP_003035333.1 CDS ECBD_1082 NC_012947.1 1129781 1131004 R TIGRFAM: diguanylate cyclase; PFAM: GGDEF domain containing protein; SMART: GGDEF domain containing protein; hypothetical protein complement(1129781..1131004) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159526 YP_003035334.1 CDS ECBD_1083 NC_012947.1 1130997 1131515 R KEGG: ssn:SSON_2755 hypothetical protein; hypothetical protein complement(1130997..1131515) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159527 YP_003035335.1 CDS ECBD_1084 NC_012947.1 1131665 1132030 R hypothetical protein complement(1131665..1132030) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159528 YP_003035336.1 CDS ECBD_1085 NC_012947.1 1132240 1133310 D catalyzes the formation of 3-deoxy-D-arabino-hept-2-ulosonate 7 phosphate from phosphoenolpyruvate and D-erythrose 4-phosphate, tyrosine sensitive; phospho-2-dehydro-3-deoxyheptonate aldolase 1132240..1133310 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159529 YP_003035337.1 CDS tyrA NC_012947.1 1133321 1134442 D catalyzes the formation of prephenate from chorismate and the formation of 4-hydroxyphenylpyruvate from prephenate in tyrosine biosynthesis; bifunctional chorismate mutase/prephenate dehydrogenase 1133321..1134442 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159530 YP_003035338.1 CDS pheA NC_012947.1 1134485 1135645 R catalyzing the formation of prephenate from chorismate and the formation of phenylpyruvate from prephenate in phenylalanine biosynthesis; bifunctional chorismate mutase/prephenate dehydratase complement(1134485..1135645) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159531 YP_003035339.1 CDS ECBD_1088 NC_012947.1 1135894 1136235 R associated with 30S ribosomal subunit; interferes with translation elongation; translation inhibitor protein RaiA complement(1135894..1136235) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159532 YP_003035340.1 CDS ECBD_1089 NC_012947.1 1136506 1137243 R with YaeT, NlpB and YfgL forms a complex involved in the proper assembly and/or targeting of OMPs to the outer membrane; involved in resistance to ampicillin and tetracycline; outer membrane protein assembly complex subunit YfiO complement(1136506..1137243) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159533 YP_003035341.1 CDS rluD NC_012947.1 1137378 1138358 D responsible for synthesis of pseudouridine from uracil at positions 1911, 1915 and 1917 in 23S ribosomal RNA; 23S rRNA pseudouridine synthase D 1137378..1138358 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159534 YP_003035342.1 CDS ECBD_1091 NC_012947.1 1138355 1139086 D PFAM: protein of unknown function DUF152; KEGG: sdy:SDY_2836 hypothetical protein; hypothetical protein 1138355..1139086 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159535 YP_003035343.1 CDS ECBD_1092 NC_012947.1 1139216 1141789 D KEGG: sbo:SBO_2627 protein disaggregation chaperone; TIGRFAM: ATP-dependent chaperone ClpB; PFAM: ATPase AAA-2 domain protein; ATPase AAA; Torsin; ATPase associated with various cellular activities AAA_5; Clp domain protein; SMART: ATPase AAA; protein disaggregation chaperone 1139216..1141789 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159536 YP_003035344.1 CDS ECBD_1093 NC_012947.1 1147558 1148856 D TIGRFAM: metabolite/H+ symporter, major facilitator superfamily (MFS); PFAM: general substrate transporter; major facilitator superfamily MFS_1; KEGG: sdy:SDY_2830 alpha-ketoglutarate transporter; alpha-ketoglutarate transporter 1147558..1148856 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159541 YP_003035345.1 CDS ECBD_1094 NC_012947.1 1148853 1149197 R KEGG: sbc:SbBS512_E2949 lipoprotein; hypothetical protein complement(1148853..1149197) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159542 YP_003035346.1 CDS pssA NC_012947.1 1149222 1150577 R catalyzes de novo synthesis of phosphatidylserine from CDP-diacylglycerol and L-serine which leads eventually to the production of phosphatidylethanolamine; bounds to the ribosome; phosphatidylserine synthase complement(1149222..1150577) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159543 YP_003035347.1 CDS ECBD_1096 NC_012947.1 1150691 1153351 R PFAM: GCN5-related N-acetyltransferase; CoA-binding domain protein; KEGG: sfx:S2819 hypothetical protein; GCN5-related N-acetyltransferase complement(1150691..1153351) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159544 YP_003035348.1 CDS ECBD_1097 NC_012947.1 1153383 1154081 R PFAM: DTW domain containing protein; KEGG: sdy:SDY_2826 hypothetical protein; DTW domain containing protein complement(1153383..1154081) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159545 YP_003035349.1 CDS ECBD_1098 NC_012947.1 1154150 1154569 R TIGRFAM: thioredoxin; PFAM: thioredoxin domain; KEGG: ssn:SSON_2708 thioredoxin 2; thioredoxin 2 complement(1154150..1154569) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159546 YP_003035350.1 CDS ECBD_1099 NC_012947.1 1154776 1155813 D PFAM: tRNA/rRNA methyltransferase (SpoU); RNA 2-O ribose methyltransferase substrate binding; KEGG: sdy:SDY_2824 predicted methyltransferase; methyltransferase 1154776..1155813 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159547 YP_003035351.1 CDS ECBD_1100 NC_012947.1 1155861 1156550 R Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine; uracil-DNA glycosylase complement(1155861..1156550) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159548 YP_003035352.1 CDS ECBD_1101 NC_012947.1 1156855 1157238 D stress-induced glycyl radical protein that can replace an oxidatively damaged pyruvate formate-lyase subunit; autonomous glycyl radical cofactor GrcA 1156855..1157238 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159549 YP_003035353.1 CDS ECBD_1102 NC_012947.1 1157294 1157881 R PFAM: lysine exporter (LYSE/YGGA); KEGG: sbc:SbBS512_E2958 translocator protein, LysE family; neutral amino-acid efflux protein complement(1157294..1157881) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159550 YP_003035354.1 CDS ECBD_1103 NC_012947.1 1157984 1158865 D PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: ssn:SSON_2703 transcriptional regulator LysR-type; LysR family transcriptional regulator 1157984..1158865 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159551 YP_003035355.1 CDS ECBD_1104 NC_012947.1 1159074 1160408 R facilitates an early step in the assembly of the 50S subunit of the ribosome; ATP-dependent RNA helicase SrmB complement(1159074..1160408) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159552 YP_003035356.1 CDS ECBD_1105 NC_012947.1 1160540 1161277 D PFAM: methyltransferase small; KEGG: sfv:SFV_2638 enzyme; methyltransferase small 1160540..1161277 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159553 YP_003035357.1 CDS ECBD_1106 NC_012947.1 1161262 1162884 R catalyzes the formation of oxaloacetate from L-aspartate; L-aspartate oxidase complement(1161262..1162884) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159554 YP_003035358.1 CDS ECBD_1107 NC_012947.1 1163140 1163295 D hypothetical protein 1163140..1163295 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159555 YP_003035359.1 CDS ECBD_1108 NC_012947.1 1163292 1163867 D Member of the extracytoplasmic function sigma factors which are active under specific conditions; binds with the catalytic core of RNA polymerase to produce the holoenzyme and directs bacterial core RNA polymerase to specific promoter elements to initiate transcription; this sigma factor is involved in heat shock and oxidative stress response; RNA polymerase sigma factor RpoE 1163292..1163867 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159556 YP_003035360.1 CDS ECBD_1109 NC_012947.1 1163900 1164550 D PFAM: anti sigma-E protein RseA family protein; anti sigma-E protein RseA domain protein; KEGG: efe:EFER_0502 anti-sigma factor; anti-RNA polymerase sigma factor SigE 1163900..1164550 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159557 YP_003035361.1 CDS rseB NC_012947.1 1164550 1165506 D periplasmic protein; interacts with the C-terminal domain of RseA and stimulates RseA binding to sigmaE via the cytoplasmic RseA N-terminal domain which then sequesters sigmaE in the membrane thereby preventing sigmaE associated with core RNAP; periplasmic negative regulator of sigmaE 1164550..1165506 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159558 YP_003035362.1 CDS ECBD_1111 NC_012947.1 1165503 1165982 D involved in the reduction of the SoxR iron-sulfur cluster; SoxR reducing system protein RseC 1165503..1165982 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159559 YP_003035363.1 CDS ECBD_1112 NC_012947.1 1166180 1167979 D binds to the ribosome on the universally-conserved alpha-sarcin loop; GTP-binding protein LepA 1166180..1167979 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159560 YP_003035364.1 CDS ECBD_1113 NC_012947.1 1167995 1168969 D catalyzes the cleavage of the amino-terminal leader peptide from secretory proteins; signal peptidase I 1167995..1168969 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159561 YP_003035365.1 CDS rnc NC_012947.1 1169242 1169922 D cytoplasmic enzyme involved in processing rRNA and some mRNAs; substrates typically have dsRNA regions; forms a homodimer; have N-terminal nuclease and C-terminal RNA-binding domains; requires magnesium as preferred ion for activity; ribonuclease III 1169242..1169922 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159562 YP_003035366.1 CDS era NC_012947.1 1169919 1170824 D Era; Escherichia coli Ras-like protein; Bex; Bacillus Era-complementing segment; essential protein in Escherichia coli that is involved in many cellular processes; GTPase; binds the cell membrane through apparent C-terminal domain; mutants are arrested during the cell cycle; Streptococcus pneumoniae Era binds to RNA and Escherichia coli Era binds 16S rRNA and 30S ribosome; GTP-binding protein Era 1169919..1170824 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159563 YP_003035367.1 CDS recO NC_012947.1 1170836 1171564 D involved in DNA repair and RecFOR pathway recombination; RecFOR proteins displace ssDNA-binding protein and facilitate the production of RecA-coated ssDNA; DNA repair protein RecO 1170836..1171564 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158994 YP_003035368.1 CDS ECBD_1117 NC_012947.1 1171576 1172307 D involved in the de novo synthesis of pyridoxine (Vitamin B6); pyridoxine 5'-phosphate synthase 1171576..1172307 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156780 YP_003035369.1 CDS acpS NC_012947.1 1172307 1172687 D Catalyzes the formation of holo-ACP, which mediates the essential transfer of acyl fatty acid intermediates during the biosynthesis of fatty acids and lipids; 4'-phosphopantetheinyl transferase 1172307..1172687 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156781 YP_003035370.1 CDS ECBD_1119 NC_012947.1 1172947 1173111 D KEGG: efe:EFER_0512 hypothetical protein; hypothetical protein 1172947..1173111 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156782 YP_003035371.1 CDS ECBD_1120 NC_012947.1 1173382 1173642 R PFAM: 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: sbc:SbBS512_E2927 4Fe-4S binding domain protein; 4Fe-4S ferredoxin iron-sulfur binding domain protein complement(1173382..1173642) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156783 YP_003035372.1 CDS ECBD_1121 NC_012947.1 1173698 1174546 R PFAM: helix-turn-helix protein RpiR; sugar isomerase (SIS); KEGG: ssn:SSON_2644 DNA-binding transcriptional regulator; DNA-binding transcriptional regulator complement(1173698..1174546) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156784 YP_003035373.1 CDS ECBD_1122 NC_012947.1 1174755 1175390 D TIGRFAM: HAD superfamily (subfamily IF) hydrolase, YfhB; KEGG: sbc:SbBS512_E2925 HAD hydrolase YhfB; hypothetical protein 1174755..1175390 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156785 YP_003035374.1 CDS ECBD_1123 NC_012947.1 1175448 1175951 D PFAM: CMP/dCMP deaminase zinc-binding; KEGG: sbc:SbBS512_E2923 tRNA-specific adenosine deaminase; tRNA-specific adenosine deaminase 1175448..1175951 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156786 YP_003035375.1 CDS ECBD_1124 NC_012947.1 1176017 1176520 R PFAM: IS1 transposase; KEGG: sty:HCM1.136a IS1 transposase; IS1 transposase complement(1176017..1176520) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156787 YP_003035376.1 CDS ECBD_1125 NC_012947.1 1176439 1176714 R PFAM: Insertion element protein; KEGG: dps:DP0861 insertion element iso-IS1d protein (InsA); Insertion element protein complement(1176439..1176714) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156788 YP_003035377.1 CDS ECBD_1126 NC_012947.1 1176748 1178280 R YfhD; uncharacterized member of the transglycosylase slt family; part of the rob operon, which plays a role in cellular resistance to antibiotics, bactericidal agents, and organic solvents; unknown function; transglycosylase complement(1176748..1178280) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156789 YP_003035378.1 CDS ECBD_1127 NC_012947.1 1178538 1182425 D catalyzes the formation of 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and L-glutamine in purine biosynthesis; phosphoribosylformylglycinamidine synthase 1178538..1182425 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156790 YP_003035379.1 CDS ECBD_1128 NC_012947.1 1183001 1184428 D PFAM: ATP-binding region ATPase domain protein; histidine kinase HAMP region domain protein; histidine kinase A domain protein; SMART: ATP-binding region ATPase domain protein; histidine kinase A domain protein; KEGG: sdy:SDY_2746 2-component sensor protein; histidine kinase 1183001..1184428 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156791 YP_003035380.1 CDS ECBD_1129 NC_012947.1 1184593 1185306 D KEGG: sfv:SFV_2603 hypothetical protein; hypothetical protein 1184593..1185306 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156792 YP_003035381.1 CDS ECBD_1130 NC_012947.1 1185296 1186630 D PFAM: sigma-54 factor interaction domain-containing protein; response regulator receiver; ATPase associated with various cellular activities AAA_5; SMART: response regulator receiver; ATPase AAA; KEGG: sbc:SbBS512_E2919 sigma-54 dependent response regulator; two component, sigma54 specific, Fis family transcriptional regulator 1185296..1186630 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156793 YP_003035382.1 CDS ECBD_1131 NC_012947.1 1186691 1187029 D indirectly regulates nitrogen metabolism; at high nitrogen levels P-II prevents the phosphorylation of NR-I, the transcriptional activator of the glutamine synthetase gene (glnA); at low nitrogen levels P-II is uridylylated to form PII-UMP and interacts with an adenylyltransferase (GlnE) that activates GlnA; nitrogen regulatory protein P-II 1 1186691..1187029 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156794 YP_003035383.1 CDS ECBD_1132 NC_012947.1 1187074 1188264 R flavohemoprotein; catalyzes the formation of nitrate from nitric oxide; can also catalyze the reduction of dihydropteridine; nitric oxide dioxygenase complement(1187074..1188264) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156795 YP_003035384.1 CDS glyA NC_012947.1 1188592 1189845 D catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate; serine hydroxymethyltransferase 1188592..1189845 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156796 YP_003035385.1 CDS ECBD_1134 NC_012947.1 1190043 1191236 R PFAM: ROK family protein; KEGG: sbo:SBO_2578 NAGC-like transcriptional regulator; ROK family protein complement(1190043..1191236) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156797 YP_003035386.1 CDS ECBD_1135 NC_012947.1 1191354 1194635 D PFAM: hypothetical protein; TPR repeat-containing protein; SMART: hypothetical protein; KEGG: sbo:SBO_2577 hypothetical protein; hypothetical protein 1191354..1194635 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156798 YP_003035387.1 CDS ECBD_1136 NC_012947.1 1194732 1195715 D PFAM: periplasmic binding protein/LacI transcriptional regulator; KEGG: sfv:SFV_2596 LacI-type transcriptional regulator; periplasmic binding protein/LacI transcriptional regulator 1194732..1195715 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156799 YP_003035388.1 CDS ECBD_1137 NC_012947.1 1195738 1197249 D PFAM: ABC transporter; SMART: ATPase AAA; KEGG: sbo:SBO_2573 ATP-binding component of a transporter; ABC transporter 1195738..1197249 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156800 YP_003035389.1 CDS ECBD_1138 NC_012947.1 1197274 1198272 D PFAM: inner-membrane translocator; KEGG: ssn:SSON_2629 transporter permease; inner-membrane translocator 1197274..1198272 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156801 YP_003035390.1 CDS ECBD_1139 NC_012947.1 1198338 1199399 D PFAM: alcohol dehydrogenase zinc-binding domain protein; alcohol dehydrogenase GroES domain protein; KEGG: ssn:SSON_2628 oxidoreductase; alcohol dehydrogenase zinc-binding domain protein 1198338..1199399 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156802 YP_003035391.1 CDS ECBD_1140 NC_012947.1 1199411 1200283 D PFAM: aldose 1-epimerase; KEGG: ssn:SSON_2627 hypothetical protein; aldose 1-epimerase 1199411..1200283 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156803 YP_003035392.1 CDS ECBD_1141 NC_012947.1 1200331 1200753 R PFAM: DoxX family protein; KEGG: sfx:S2762 hypothetical protein; DoxX family protein complement(1200331..1200753) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156804 YP_003035393.1 CDS ECBD_1142 NC_012947.1 1200850 1202052 R PFAM: FAD-dependent pyridine nucleotide-disulphide oxidoreductase; KEGG: ssn:SSON_2624 phenylpropionate dioxygenase, ferredoxin reductase subunit; phenylpropionate dioxygenase ferredoxin reductase subunit complement(1200850..1202052) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156805 YP_003035394.1 CDS ECBD_1143 NC_012947.1 1202062 1202874 R Converts cis-3-(3-carboxyethyl)-3,5-cyclohexadiene-1,2-diol (PP-dihydrodiol) into 3-(2,3-dihydroxylphenyl)propionate; 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase complement(1202062..1202874) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156806 YP_003035395.1 CDS ECBD_1144 NC_012947.1 1202871 1203191 R PFAM: Rieske [2Fe-2S] domain protein; KEGG: ssn:SSON_2622 3-phenylpropionate dioxygenase ferredoxin subunit; 3-phenylpropionate dioxygenase ferredoxin subunit complement(1202871..1203191) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156807 YP_003035396.1 CDS ECBD_1145 NC_012947.1 1203191 1203709 R PFAM: aromatic-ring-hydroxylating dioxygenase beta subunit; KEGG: sfx:S2758 3-phenylpropionate dioxygenase subunit beta; 3-phenylpropionate dioxygenase subunit beta complement(1203191..1203709) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156808 YP_003035397.1 CDS ECBD_1146 NC_012947.1 1203706 1205067 R PFAM: Aromatic-ring-hydroxylating dioxygenase, alpha subunit-like; Rieske [2Fe-2S] domain protein; KEGG: ssn:SSON_2620 large terminal subunit of phenylpropionate dioxygenase; Aromatic-ring-hydroxylating dioxygenase, alpha subunit-like protein complement(1203706..1205067) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156809 YP_003035398.1 CDS ECBD_1147 NC_012947.1 1205203 1206093 D activator of 3-phenylpropionic acid catabolism; DNA-binding transcriptional regulator HcaR 1205203..1206093 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156810 YP_003035399.1 CDS ECBD_1148 NC_012947.1 1206253 1207392 D transporter of 3-phenylpropionate across the inner membrane; member of the major facilitator superfamily of transporters; 3-phenylpropionic acid transporter 1206253..1207392 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156811 YP_003035400.1 CDS ECBD_1149 NC_012947.1 1207384 1208664 R PFAM: PRD domain protein; KEGG: sbo:SBO_2559 stationary phase inducible protein CsiE; stationary phase inducible protein CsiE complement(1207384..1208664) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156812 YP_003035401.1 CDS ECBD_1150 NC_012947.1 1208855 1209709 R KEGG: sdy:SDY_2730 enzyme; enzyme complement(1208855..1209709) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156813 YP_003035402.1 CDS ECBD_1151 NC_012947.1 1209854 1210657 R PFAM: inositol monophosphatase; KEGG: sbo:SBO_2557 inositol monophosphatase; inositol monophosphatase complement(1209854..1210657) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156814 YP_003035403.1 CDS ECBD_1152 NC_012947.1 1210776 1211516 D TIGRFAM: RNA methyltransferase, TrmH family, group 1; PFAM: tRNA/rRNA methyltransferase (SpoU); KEGG: sfv:SFV_2580 ATP synthase subunit beta; RNA methyltransferase, TrmH family, group 1 1210776..1211516 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156815 YP_003035404.1 CDS ECBD_1153 NC_012947.1 1211968 1212456 D regulates the expression of the iscRSUA operon; DNA-binding transcriptional regulator IscR 1211968..1212456 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156816 YP_003035405.1 CDS ECBD_1154 NC_012947.1 1212568 1213782 D catalyzes the removal of elemental sulfur from cysteine to produce alanine; involved in NAD biosynthesis; cysteine desulfurase 1212568..1213782 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156817 YP_003035406.1 CDS ECBD_1155 NC_012947.1 1213810 1214196 D TIGRFAM: FeS cluster assembly scaffold IscU; PFAM: nitrogen-fixing NifU domain protein; KEGG: ssn:SSON_2611 scaffold protein; scaffold protein 1213810..1214196 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156818 YP_003035407.1 CDS iscA NC_012947.1 1214213 1214536 D forms iron-sulfur clusters of ferredoxin [2FE-2S]; binds iron in the presence of the thioredoxin reductase system; forms homodimers and tetramers; similar to SufA protein; iron-sulfur cluster assembly protein 1214213..1214536 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156819 YP_003035408.1 CDS hscB NC_012947.1 1214632 1215147 D J-type co-chaperone that regulates the ATPase and peptide-binding activity of Hsc66 chaperone; may function in biogenesis of iron-sulfur proteins; co-chaperone HscB 1214632..1215147 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156820 YP_003035409.1 CDS hscA NC_012947.1 1215164 1217014 D involved in the maturation of iron-sulfur cluster-containing proteins; chaperone protein HscA 1215164..1217014 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156821 YP_003035410.1 CDS ECBD_1159 NC_012947.1 1217016 1217351 D TIGRFAM: ferredoxin 2Fe-2S type, ISC system; PFAM: ferredoxin; KEGG: sbc:SbBS512_E2900 ferredoxin 2Fe-2S type, ISC system; ferredoxin 2Fe-2S type, ISC system 1217016..1217351 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156822 YP_003035411.1 CDS ECBD_1160 NC_012947.1 1217363 1217563 D TIGRFAM: FeS assembly protein IscX; PFAM: protein of unknown function DUF528; KEGG: sdy:SDY_2720 hypothetical protein; hypothetical protein 1217363..1217563 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156823 YP_003035412.1 CDS ECBD_1161 NC_012947.1 1217621 1218904 D catalyzes the removal of an N-terminal amino acid from a peptide or arylamide; aminopeptidase B 1217621..1218904 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156824 YP_003035413.1 CDS ECBD_1162 NC_012947.1 1219046 1219822 D enhances serine sensitivity caused by inhibition of homoserine dehydrogenase I; enhanced serine sensitivity protein SseB 1219046..1219822 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156825 YP_003035414.1 CDS ECBD_1163 NC_012947.1 1220644 1221483 R PFAM: Integrase catalytic region; KEGG: bcj:BCAS0612 integrase; Integrase catalytic region complement(1220644..1221483) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156826 YP_003035415.1 CDS ECBD_1164 NC_012947.1 1221480 1222001 R KEGG: pnu:Pnuc_1431 ISPsy8, transposase OrfA; ISPsy8, transposase OrfA complement(1221480..1222001) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156827 YP_003035416.1 CDS sseA NC_012947.1 1222085 1222930 R catalyzes the transfer of a sulfur ion to cyanide or to other thiol compounds; 3-mercaptopyruvate sulfurtransferase complement(1222085..1222930) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156828 YP_003035417.1 CDS ECBD_1166 NC_012947.1 1223137 1228098 D PFAM: alpha-2-macroglobulin; alpha-2-macroglobulin 2; KEGG: ssn:SSON_2602 hypothetical protein; alpha-2-macroglobulin 1223137..1228098 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156829 YP_003035418.1 CDS ECBD_1167 NC_012947.1 1228099 1230411 D penicillin-insensitive transglycosylase/transpeptidase; penicillin-binding protein 1C 1228099..1230411 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156830 YP_003035419.1 CDS ndk NC_012947.1 1230560 1230991 D catalyzes the formation of nucleoside triphosphate from ATP and nucleoside diphosphate; nucleoside diphosphate kinase 1230560..1230991 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156831 YP_003035420.1 CDS ECBD_1169 NC_012947.1 1231141 1232295 D 23S rRNA m2A2503 methyltransferase; methylates the C2 position of the A2530 nucleotide in 23S rRNA; may be involved in antibiotic resistance; ribosomal RNA large subunit methyltransferase N 1231141..1232295 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156832 YP_003035421.1 CDS ECBD_1170 NC_012947.1 1232580 1233593 D PFAM: helix-turn-helix domain protein; SMART: helix-turn-helix domain protein; KEGG: sbc:SbBS512_E2891 helix-turn-helix DNA-binding domain protein; cytoskeletal protein RodZ 1232580..1233593 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156833 YP_003035422.1 CDS ispG NC_012947.1 1233620 1234738 D catalyzes the conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate into 4-hydroxy-3-methyl-2-en-1-yl diphosphate; involved in isoprenoid synthesis; 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase 1233620..1234738 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156834 YP_003035423.1 CDS hisS NC_012947.1 1234849 1236123 D catalyzes a two-step reaction, first charging a histidine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class II aminoacyl-tRNA synthetase; forms homodimers; some organisms have a paralogous gene, hisZ, that is similar to hisS and produces a protein that performs the first step in histidine biosynthesis along with HisG; histidyl-tRNA synthetase 1234849..1236123 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156835 YP_003035424.1 CDS ECBD_1173 NC_012947.1 1236141 1236761 D KEGG: sdy:SDY_2709 hypothetical protein; hypothetical protein 1236141..1236761 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156836 YP_003035425.1 CDS ECBD_1174 NC_012947.1 1236772 1237950 D with YaeT, YfiO, and NlpB forms a complex involved in outer membrane protein biogenesis; outer membrane protein assembly complex subunit YfgL 1236772..1237950 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156837 YP_003035426.1 CDS engA NC_012947.1 1238068 1239540 D EngA; essential Neisserial GTPase; synchronizes cellular events by interacting with multiple targets with tandem G-domains; overexpression in Escherichia coli suppresses rrmJ mutation; structural analysis of the Thermotoga maritima ortholog shows different nucleotide binding affinities in the two binding domains; GTP-binding protein EngA 1238068..1239540 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156838 YP_003035427.1 CDS ECBD_1176 NC_012947.1 1239610 1239825 D PFAM: protein of unknown function DUF1407; KEGG: sfv:SFV_2557 hypothetical protein; hypothetical protein 1239610..1239825 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156839 YP_003035428.1 CDS xseA NC_012947.1 1239822 1241192 R bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides; exodeoxyribonuclease VII large subunit complement(1239822..1241192) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156840 YP_003035429.1 CDS ECBD_1178 NC_012947.1 1241354 1242820 D catalyzes the synthesis of xanthosine monophosphate by the NAD+ dependent oxidation of inosine monophosphate; inosine 5'-monophosphate dehydrogenase 1241354..1242820 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156841 YP_003035430.1 CDS guaA NC_012947.1 1242889 1244466 D contains glutamine-hydrolyzing domain and glutamine amidotransferase; GMP-binding domain; functions to produce GMP from XMP in the IMP pathway; GMP synthase 1242889..1244466 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156842 YP_003035431.1 CDS ECBD_1180 NC_012947.1 1244559 1245098 R hypothetical protein complement(1244559..1245098) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156843 YP_003035432.1 CDS ECBD_1181 NC_012947.1 1245114 1245629 R PFAM: 17 kDa surface antigen; KEGG: elf:LF82_3095 uncharacterized lipoprotein YfgH; 17 kDa surface antigen complement(1245114..1245629) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156844 YP_003035433.1 CDS ECBD_1182 NC_012947.1 1245735 1245881 R hypothetical protein complement(1245735..1245881) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156845 YP_003035434.1 CDS ECBD_1183 NC_012947.1 1245943 1246134 R KEGG: ssn:SSON_2586 hypothetical protein; hypothetical protein complement(1245943..1246134) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156846 YP_003035435.1 CDS ECBD_1184 NC_012947.1 1246152 1246304 D hypothetical protein 1246152..1246304 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156847 YP_003035436.1 CDS ECBD_1185 NC_012947.1 1246486 1248729 D PFAM: MASE1 domain protein; EAL domain protein; SMART: EAL domain protein; GGDEF domain containing protein; KEGG: ssn:SSON_2585 cytochrome C biogenesis protein; diguanylate cyclase/phosphodiesterase 1246486..1248729 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156848 YP_003035437.1 CDS ECBD_1186 NC_012947.1 1248768 1250309 R PFAM: Ppx/GppA phosphatase; KEGG: ssn:SSON_2584 exopolyphosphatase; exopolyphosphatase complement(1248768..1250309) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156849 YP_003035438.1 CDS ECBD_1187 NC_012947.1 1250314 1252380 R catalyzes the reversible transfer of the terminal phosphate of ATP to form a long chain polyphosphate; polyphosphate kinase complement(1250314..1252380) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156850 YP_003035439.1 CDS purN NC_012947.1 1252551 1253189 R glycinamide ribonucleotide transformylase; GAR Tfase; catalyzes the synthesis of 5'-phosphoribosylformylglycinamide from 5'-phosphoribosylglycinamide and 10-formyltetrahydrofolate; PurN requires formyl folate for the reaction unlike PurT which uses formate; phosphoribosylglycinamide formyltransferase complement(1252551..1253189) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156851 YP_003035440.1 CDS ECBD_1189 NC_012947.1 1253189 1254226 R catalyzes the formation of 1-(5-phosphoribosyl)-5-aminoimidazole from 2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in purine biosynthesis; phosphoribosylaminoimidazole synthetase complement(1253189..1254226) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156852 YP_003035441.1 CDS upp NC_012947.1 1254551 1255177 D Catalyzes the formation of uracil and 5-phospho-alpha-D-ribosy 1-diphosphate from UMP and diphosphate; uracil phosphoribosyltransferase 1254551..1255177 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156853 YP_003035442.1 CDS ECBD_1191 NC_012947.1 1255263 1256552 D TIGRFAM: uracil-xanthine permease; PFAM: xanthine/uracil/vitamin C permease; KEGG: ssn:SSON_2579 uracil transporter; uracil transporter 1255263..1256552 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156854 YP_003035443.1 CDS ECBD_1192 NC_012947.1 1256602 1257348 D controls initiation of DNA replication by inhibiting re-initiation of replication, promotes hydrolysis of DnaA-bound ATP; DNA replication initiation factor 1256602..1257348 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156855 YP_003035444.1 CDS ECBD_1193 NC_012947.1 1257486 1257845 R TIGRFAM: arsenate reductase; PFAM: arsenate reductase and related; KEGG: sdy:SDY_2684 oxidoreductase; arsenate reductase complement(1257486..1257845) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156856 YP_003035445.1 CDS ECBD_1194 NC_012947.1 1257866 1259329 R PFAM: peptidase M48 Ste24p; KEGG: sfv:SFV_2539 hypothetical protein; peptidase M48 Ste24p complement(1257866..1259329) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156857 YP_003035446.1 CDS ECBD_1195 NC_012947.1 1259542 1260603 D PFAM: protein of unknown function UPF0118; KEGG: sdy:SDY_2682 permease; hypothetical protein 1259542..1260603 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156858 YP_003035447.1 CDS focB NC_012947.1 1260641 1261489 R inner membrane protein presumed to act as a formate transporter; member of the hyf operon; formate transporter complement(1260641..1261489) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156859 YP_003035448.1 CDS ECBD_1197 NC_012947.1 1261511 1263523 R PFAM: sigma-54 factor interaction domain-containing protein; helix-turn-helix Fis-type; GAF domain protein; SMART: GAF domain protein; ATPase AAA; KEGG: ssn:SSON_2572 2-component regulator, interaction with sigma 54; Fis family transcriptional regulator complement(1261511..1263523) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156860 YP_003035449.1 CDS ECBD_1198 NC_012947.1 1263553 1263966 R PFAM: formate hydrogenlyase maturation HycH; KEGG: sfl:SF2534 protein processing element; formate hydrogenlyase maturation HycH complement(1263553..1263966) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158487 YP_003035450.1 CDS ECBD_1199 NC_012947.1 1263959 1264717 R PFAM: NADH ubiquinone oxidoreductase 20 kDa subunit; KEGG: ssn:SSON_2570 hydrogenase 4 Fe-S subunit; NADH ubiquinone oxidoreductase 20 kDa subunit complement(1263959..1264717) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157343 YP_003035451.1 CDS ECBD_1200 NC_012947.1 1264714 1265253 R probable electron transfer protein for hydrogenase 4; hydrogenase 4 is believed to be silent in E. coli under conditions tested so far; hydrogenase 4 subunit H complement(1264714..1265253) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157344 YP_003035452.1 CDS ECBD_1201 NC_012947.1 1265263 1266930 R PFAM: NADH dehydrogenase (ubiquinone) 30 kDa subunit; KEGG: ssn:SSON_2568 hydrogenase 4 subunit; NADH dehydrogenase (ubiquinone) 30 kDa subunit complement(1265263..1266930) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157345 YP_003035453.1 CDS ECBD_1202 NC_012947.1 1266920 1268500 R PFAM: NADH/Ubiquinone/plastoquinone (complex I); KEGG: ssn:SSON_2567 hydrogenase 4 subunit F; hydrogenase 4 subunit F complement(1266920..1268500) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157346 YP_003035454.1 CDS hyfE NC_012947.1 1268505 1269155 R interacts with formate dehydrogenase to produce an active formate hydrogenlyase complex which cleaves formate to dihydrogen and carbon dioxide; hydrogenase 4 membrane subunit complement(1268505..1269155) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157347 YP_003035455.1 CDS ECBD_1204 NC_012947.1 1269170 1270519 R PFAM: NADH/Ubiquinone/plastoquinone (complex I); KEGG: sfv:SFV_2529 hydrogenase 4 subunit D; hydrogenase 4 subunit D complement(1269170..1270519) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157348 YP_003035456.1 CDS ECBD_1205 NC_012947.1 1270495 1270605 R KEGG: sfx:S2677 hydrogenase 4 subunit D; hydrogenase 4 subunit D complement(1270495..1270605) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157349 YP_003035457.1 CDS ECBD_1207 NC_012947.1 1271579 1273597 R PFAM: NADH/Ubiquinone/plastoquinone (complex I); KEGG: ssn:SSON_2563 hydrogenase 4 subunit B; hydrogenase 4 subunit B complement(1271579..1273597) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157351 YP_003035458.1 CDS ECBD_1208 NC_012947.1 1273597 1274214 R PFAM: 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: sfv:SFV_2526 hydrogenase 4 Fe-S subunit; 4Fe-4S ferredoxin iron-sulfur binding domain protein complement(1273597..1274214) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157352 YP_003035459.1 CDS bcp NC_012947.1 1274467 1274937 R bacterioferritin comigratory protein; thiol peroxidase; thioredoxin-dependent; hydroperoxide peroxidase; in Escherichia coli this enzyme preferentially reduces linoleic acid hydroperoxide; contains an active site cysteine; thioredoxin-dependent thiol peroxidase complement(1274467..1274937) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157353 YP_003035460.1 CDS gcvR NC_012947.1 1274937 1275509 R negative transcriptional regulator of the gcvTHP operon; glycine cleavage system transcriptional repressor complement(1274937..1275509) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157354 YP_003035461.1 CDS ECBD_1211 NC_012947.1 1275655 1276533 D catalyzes the formation of dihydrodipicolinate from L-aspartate 4-semialdehyde and pyruvate in lysine and diaminopimelate biosynthesis; dihydrodipicolinate synthase 1275655..1276533 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157355 YP_003035462.1 CDS ECBD_1212 NC_012947.1 1276550 1277584 D PFAM: NlpBDapX family lipoprotein; KEGG: ssn:SSON_2558 lipoprotein; lipoprotein 1276550..1277584 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157356 YP_003035463.1 CDS ECBD_1213 NC_012947.1 1277797 1278510 D catalyzes the formation of (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate and L-aspartate in purine biosynthesis; SAICAR synthase; phosphoribosylaminoimidazole-succinocarboxamide synthase 1277797..1278510 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157357 YP_003035464.1 CDS ECBD_1214 NC_012947.1 1278678 1279541 D PFAM: protein of unknown function zinc metallopeptidase KEGG: sfv:SFV_2520 hypothetical protein; hypothetical protein 1278678..1279541 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157358 YP_003035465.1 CDS ECBD_1215 NC_012947.1 1279556 1281571 D PFAM: protein of unknown function DUF699 ATPase ; GCN5-related N-acetyltransferase; KEGG: sfx:S2667 hypothetical protein; hypothetical protein 1279556..1281571 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157359 YP_003035466.1 CDS ECBD_1216 NC_012947.1 1281645 1282343 D Displays esterase activity toward palmitoyl-CoA and pNP-butyrate; esterase YpfH 1281645..1282343 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157360 YP_003035467.1 CDS ECBD_1217 NC_012947.1 1282453 1282653 R KEGG: ssn:SSON_2553 inner membrane protein; hypothetical protein complement(1282453..1282653) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157361 YP_003035468.1 CDS ECBD_1218 NC_012947.1 1282681 1283808 R dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE), catalyzes the hydrolysis of N-succinyl-L,Ldiaminopimelate L,L-SDAP to L,L-diaminopimelate and succinate. It is a metalloprotease containing dinuclear active sites. Its structure is similar to the carboxypeptidase G2 from Pseudomonas sp. strain RS-16 and the aminopeptidase from Aeromonas proteolytica.; succinyl-diaminopimelate desuccinylase complement(1282681..1283808) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157362 YP_003035469.1 CDS ECBD_1219 NC_012947.1 1283812 1284168 R PFAM: arsenate reductase; KEGG: ssn:SSON_2551 hypothetical protein; hypothetical protein complement(1283812..1284168) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157363 YP_003035470.1 CDS ECBD_1220 NC_012947.1 1284707 1287820 R TIGRFAM: transporter hydrophobe/amphiphile efflux-1 (HAE1) family; PFAM: acriflavin resistance protein; aminoglycoside/multidrug efflux system complement(1284707..1287820) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157364 YP_003035471.1 CDS ECBD_1221 NC_012947.1 1287984 1289684 R KEGG: sdy:SDY_2653 nitrate/nitrite sensor protein NarQ; PFAM: histidine kinase dimerisation and phosphoacceptor region; ATP-binding region ATPase domain protein; histidine kinase HAMP region domain protein; SMART: ATP-binding region ATPase domain protein; histidine kinase HAMP region domain protein; nitrate/nitrite sensor protein NarQ complement(1287984..1289684) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157365 YP_003035472.1 CDS ECBD_1222 NC_012947.1 1289890 1291869 D unknown function; in E. coli the aegA gene is regulated by Fnr, NarXL, and NarQ (but not ArcA), induced under anaerobic conditions and repressed in the presence of nitrate (anaerobic); oxidoreductase Fe-S binding subunit 1289890..1291869 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157366 YP_003035473.1 CDS ECBD_1223 NC_012947.1 1291937 1292512 D PFAM: NUDIX hydrolase; KEGG: ssn:SSON_2547 hypothetical protein; NUDIX hydrolase 1291937..1292512 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157367 YP_003035474.1 CDS ECBD_1224 NC_012947.1 1292638 1293681 D PFAM: protein of unknown function DUF1176; KEGG: sbo:SBO_2482 hypothetical protein; hypothetical protein 1292638..1293681 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157368 YP_003035475.1 CDS ECBD_1225 NC_012947.1 1293776 1295779 R catalyzes the formation of ribose 5-phosphate and xylulose 5-phosphate from sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate; can transfer ketol groups between several groups; in Escherichia coli there are two tkt genes, tktA expressed during exponential growth and the tktB during stationary phase; transketolase complement(1293776..1295779) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157369 YP_003035476.1 CDS ECBD_1226 NC_012947.1 1295799 1296749 R KEGG: elf:LF82_2215 transaldolase A; TIGRFAM: transaldolase; PFAM: transaldolase; transaldolase A complement(1295799..1296749) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157370 YP_003035477.1 CDS ECBD_1227 NC_012947.1 1297038 1299317 D NADP-dependent; catalyzes the oxidative decarboxylation of malate to form pyruvate; decarboxylates oxaloacetate; malic enzyme 1297038..1299317 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157371 YP_003035478.1 CDS ECBD_1228 NC_012947.1 1299610 1299945 D PFAM: microcompartments protein; KEGG: sfv:SFV_2506 hypothetical protein; microcompartments protein 1299610..1299945 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157372 YP_003035479.1 CDS ECBD_1229 NC_012947.1 1299958 1300437 D TIGRFAM: ethanolamine utilization protein, EutP; KEGG: sbc:SbBS512_E2827 ethanolamine utilization protein, EutP; ethanolamine utilization protein, EutP 1299958..1300437 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157373 YP_003035480.1 CDS ECBD_1230 NC_012947.1 1300412 1301113 D PFAM: ethanolamine utilisation EutQ family protein; protein of unknown function DUF861 cupin_3; KEGG: ssn:SSON_2540 hypothetical protein; ethanolamine utilisation EutQ family protein 1300412..1301113 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157374 YP_003035481.1 CDS ECBD_1231 NC_012947.1 1301110 1301919 D PFAM: cobalamin adenosyltransferase; KEGG: sbc:SbBS512_E2825 ethanolamine utilization cobalamin adenosyltransferase; Cob(I)yrinic acid a,c-diamide adenosyltransferase 1301110..1301919 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157375 YP_003035482.1 CDS eutD NC_012947.1 1301909 1302925 D in Salmonella this enzyme is required for ethanolamine catabolism; has higher affinity for CoA than Pta; phosphotransacetylase 1301909..1302925 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157376 YP_003035483.1 CDS ECBD_1233 NC_012947.1 1302964 1303257 D PFAM: microcompartments protein; KEGG: sbc:SbBS512_E2823 ethanolamine utilization protein EutM; microcompartments protein 1302964..1303257 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157377 YP_003035484.1 CDS ECBD_1234 NC_012947.1 1303364 1303651 D PFAM: ethanolamine utilization protein EutN/carboxysome structural protein Ccml; KEGG: sbc:SbBS512_E2822 ethanolamine utilization protein; ethanolamine utilization protein EutN/carboxysome structural protein Ccml 1303364..1303651 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157378 YP_003035485.1 CDS ECBD_1235 NC_012947.1 1303663 1305066 D PFAM: aldehyde dehydrogenase; KEGG: sbo:SBO_2470 ethanolamine utilization; aldehyde dehydrogenase 1303663..1305066 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157379 YP_003035486.1 CDS ECBD_1236 NC_012947.1 1305077 1305913 D TIGRFAM: ethanolamine utilization protein EutJ family protein; KEGG: sbc:SbBS512_E2820 ethanolamine utilization protein EutJ; ethanolamine utilization protein EutJ family protein 1305077..1305913 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157380 YP_003035487.1 CDS ECBD_1237 NC_012947.1 1305903 1307090 D PFAM: iron-containing alcohol dehydrogenase; KEGG: ssn:SSON_2533 EutG; iron-containing alcohol dehydrogenase 1305903..1307090 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157381 YP_003035488.1 CDS ECBD_1238 NC_012947.1 1307191 1307391 D hypothetical protein 1307191..1307391 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157382 YP_003035489.1 CDS ECBD_1239 NC_012947.1 1307407 1308633 D PFAM: ethanolamine utilisation protein EutH; KEGG: ssn:SSON_2532 EutH; ethanolamine utilisation protein EutH 1307407..1308633 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157383 YP_003035490.1 CDS eutA NC_012947.1 1308630 1310033 D ethanolamine utilization protein EutA; reactivating factor for ethanolamine ammonia lyase 1308630..1310033 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157384 YP_003035491.1 CDS ECBD_1241 NC_012947.1 1310045 1311406 D PFAM: ethanolamine ammonia lyase large subunit; KEGG: sbc:SbBS512_E2816 ethanolamine ammonia-lyase, large subunit; ethanolamine ammonia-lyase 1310045..1311406 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157385 YP_003035492.1 CDS ECBD_1242 NC_012947.1 1311427 1312314 D catalyzes the formation of acetaldehyde from ethanolamine; ethanolamine ammonia-lyase small subunit 1311427..1312314 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157386 YP_003035493.1 CDS ECBD_1243 NC_012947.1 1312324 1312983 D PFAM: microcompartments protein; KEGG: sbc:SbBS512_E2811 ethanolamine utilization protein EutL; microcompartments protein 1312324..1312983 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157387 YP_003035494.1 CDS ECBD_1244 NC_012947.1 1312996 1313496 D PFAM: microcompartments protein; KEGG: sbc:SbBS512_E2810 ethanolamine utilization protein EutK; microcompartments protein 1312996..1313496 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157388 YP_003035495.1 CDS ECBD_1245 NC_012947.1 1313542 1314594 D activates the transcription of the ethanolamine utilization operon; transcriptional regulator EutR 1313542..1314594 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157389 YP_003035496.1 CDS ECBD_1246 NC_012947.1 1314600 1315499 R catalyzes the conversion of the propionic acid groups of rings I and III to vinyl groups during heme synthesis; coproporphyrinogen III oxidase complement(1314600..1315499) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157390 YP_003035497.1 CDS ECBD_1247 NC_012947.1 1315503 1316372 R KEGG: ssn:SSON_2524 N-acetylmuramoyl-L-alanine amidase I; PFAM: cell wall hydrolase/autolysin; SMART: cell wall hydrolase/autolysin; N-acetylmuramoyl-l-alanine amidase I complement(1315503..1316372) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157391 YP_003035498.1 CDS ECBD_1248 NC_012947.1 1316586 1317011 D PFAM: GCN5-related N-acetyltransferase; KEGG: ssn:SSON_2523 acetyltransferase; acetyltransferase 1316586..1317011 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157392 YP_003035499.1 CDS ECBD_1249 NC_012947.1 1316998 1317447 D KEGG: ssn:SSON_2522 hypothetical protein; hypothetical protein 1316998..1317447 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157393 YP_003035500.1 CDS ECBD_1250 NC_012947.1 1317654 1318229 D PFAM: protein of unknown function DUF1131; KEGG: sbc:SbBS512_E2804 lipoprotein; hypothetical protein 1317654..1318229 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157394 YP_003035501.1 CDS ECBD_1251 NC_012947.1 1318325 1319224 D TIGRFAM: Dyp-type peroxidase family; PFAM: Dyp-type peroxidase; KEGG: sbc:SbBS512_E2803 dyp-type peroxidase family protein; Dyp-type peroxidase family 1318325..1319224 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157395 YP_003035502.1 CDS murP NC_012947.1 1319404 1320828 R belongs to PEP-dependent PTS system; contains the PTS EIIBC domains; involved in uptake of exogenous N-acetylmuramic acid (MurNAc); requires crr-encoded enzyme IIA-glucose component; PTS system N-acetylmuramic acid transporter subunits EIIBC complement(1319404..1320828) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157396 YP_003035503.1 CDS murQ NC_012947.1 1320832 1321728 R catalyzes the cleavage of the lactyl ether moiety of N-acetylmuramic acid-6-phosphate (MurNAc-6-P) to form N-acetylglucosamine-6-phosphate (GlcNAc-6-P) and lactate; involved in MurNAc dissimilation pathway; N-acetylmuramic acid-6-phosphate etherase complement(1320832..1321728) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157397 YP_003035504.1 CDS ECBD_1254 NC_012947.1 1321892 1322749 D PFAM: helix-turn-helix protein RpiR; sugar isomerase (SIS); KEGG: sbc:SbBS512_E2799 RpiR family transcriptional regulator; RpiR family transcriptional regulator 1321892..1322749 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157398 YP_003035505.1 CDS ECBD_1255 NC_012947.1 1322878 1323669 D PFAM: short-chain dehydrogenase/reductase SDR; KR domain protein; NAD-dependent epimerase/dehydratase; KEGG: ssn:SSON_2515 short chain dehydrogenase; short chain dehydrogenase 1322878..1323669 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157399 YP_003035506.1 CDS ECBD_1256 NC_012947.1 1323973 1324989 D TIGRFAM: sulfate ABC transporter periplasmic sulfate-binding protein; PFAM: extracellular solute-binding protein family 1; KEGG: sdy:SDY_2621 thiosulfate transporter subunit; thiosulfate transporter subunit 1323973..1324989 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157400 YP_003035507.1 CDS ECBD_1257 NC_012947.1 1324989 1325822 D TIGRFAM: sulfate ABC transporter inner membrane subunit; sulfate ABC transporter inner membrane subunit CysT; PFAM: binding-protein-dependent transporters inner membrane component; KEGG: sfv:SFV_2477 sulfate/thiosulfate transporter subunit; sulfate/thiosulfate transporter subunit 1324989..1325822 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157401 YP_003035508.1 CDS cysW NC_012947.1 1325822 1326697 D Part of the ABC transporter complex cysAWTP involved in sulfate/thiosulfate import; sulfate/thiosulfate transporter permease subunit 1325822..1326697 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157402 YP_003035509.1 CDS ECBD_1259 NC_012947.1 1326687 1327784 D KEGG: sbo:SBO_2447 sulfate/thiosulfate transporter subunit; TIGRFAM: sulfate ABC transporter ATPase; PFAM: ABC transporter related; SMART: ATPase AAA; sulfate/thiosulfate transporter subunit 1326687..1327784 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157403 YP_003035510.1 CDS cysM NC_012947.1 1327918 1328829 D catalyzes the formation of cysteine from 3-O-acetyl-L-serine and hydrogen sulfide; cysteine synthase B 1327918..1328829 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157404 YP_003035511.1 CDS ECBD_1261 NC_012947.1 1329034 1329768 R PFAM: WGR domain protein; SMART: WGR domain protein; KEGG: sfv:SFV_2472 hypothetical protein; WGR domain protein complement(1329034..1329768) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157405 YP_003035512.1 CDS ECBD_1262 NC_012947.1 1329801 1330175 R KEGG: sfv:SFV_2471 hypothetical protein; hypothetical protein complement(1329801..1330175) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157406 YP_003035513.1 CDS pdxK NC_012947.1 1330280 1331131 D catalyzes the formation of pyridoxal 5'-phosphate from pyridoxal; pyridoxal kinase 1330280..1331131 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157407 YP_003035514.1 CDS ECBD_1264 NC_012947.1 1331174 1331683 R functions in phosphoenolpyruvate-(PEP)-dependent phosphotransferase (PTS) system; functions in the transport and phosphorylation of glucose; PTS system glucose-specific transporter subunit complement(1331174..1331683) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157408 YP_003035515.1 CDS ECBD_1265 NC_012947.1 1331724 1333451 R phosphotransferase system, enzyme I; transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein; part of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active-transporter; phosphoenolpyruvate-protein phosphotransferase complement(1331724..1333451) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157409 YP_003035516.1 CDS ECBD_1266 NC_012947.1 1333496 1333753 R TIGRFAM: phosphocarrier, HPr family; PFAM: phosphoryl transfer system HPr; KEGG: sea:SeAg_B2576 phosphohistidinoprotein-hexose phosphotransferase component of PTS system (HPr); PTS system phosphohistidinoprotein-hexose phosphotransferase subunit Hpr complement(1333496..1333753) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157410 YP_003035517.1 CDS ECBD_1267 NC_012947.1 1334137 1335108 R CysK; forms a complex with serine acetyltransferase CysE; functions in cysteine biosynthesis; cysteine synthase A complement(1334137..1335108) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157411 YP_003035518.1 CDS ECBD_1268 NC_012947.1 1335101 1335265 R hypothetical protein complement(1335101..1335265) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157412 YP_003035519.1 CDS ECBD_1269 NC_012947.1 1335293 1336054 R role in sulfur assimilation; sulfate transporter CysZ complement(1335293..1336054) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157413 YP_003035520.1 CDS ECBD_1270 NC_012947.1 1336284 1337270 D interacts with cell division protein FitsZ and may be required to anchor septal ring structure; cell division protein ZipA 1336284..1337270 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157414 YP_003035521.1 CDS ligA NC_012947.1 1337341 1339356 D this protein catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction; essential for DNA replication and repair of damaged DNA; similar to ligase LigB; NAD-dependent DNA ligase LigA 1337341..1339356 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157415 YP_003035522.1 CDS ECBD_1272 NC_012947.1 1339358 1339576 D KEGG: ssn:SSON_2499 cytoplasmic protein; cytoplasmic protein 1339358..1339576 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157416 YP_003035523.1 CDS ECBD_1273 NC_012947.1 1339573 1340571 R PFAM: sodium symporter; sodium symporter complement(1339573..1340571) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157417 YP_003035524.1 CDS ECBD_1274 NC_012947.1 1340661 1341587 D PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: sbc:SbBS512_E2773 LysR family transcriptional regulator; LysR family transcriptional regulator 1340661..1341587 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157418 YP_003035525.1 CDS ECBD_1275 NC_012947.1 1341578 1341919 R KEGG: sfl:SF2463 hypothetical protein; hypothetical protein complement(1341578..1341919) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157419 YP_003035526.1 CDS gltX NC_012947.1 1342695 1344110 D Charges one glutamine molecule and pairs it to its corresponding RNA trinucleotide during protein translation; glutamyl-tRNA synthetase 1342695..1344110 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157910 YP_003035527.1 CDS ECBD_1277 NC_012947.1 1344162 1344554 R PFAM: protein of unknown function DUF1323; KEGG: sbc:SbBS512_E2780 hypothetical protein; hypothetical protein complement(1344162..1344554) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157911 YP_003035528.1 CDS ECBD_1278 NC_012947.1 1344556 1344900 R PFAM: protein of unknown function DUF1323; KEGG: ssn:SSON_2489 hypothetical protein; hypothetical protein complement(1344556..1344900) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157912 YP_003035529.1 CDS ECBD_1279 NC_012947.1 1345535 1347724 D PFAM: EAL domain protein; GGDEF domain containing protein; MASE1 domain protein; SMART: EAL domain protein; GGDEF domain containing protein; KEGG: sbo:SBO_2421 hypothetical protein; diguanylate cyclase/phosphodiesterase 1345535..1347724 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157915 YP_003035530.1 CDS ECBD_1280 NC_012947.1 1347774 1348976 R TIGRFAM: nucleoside transporter; PFAM: Na+ dependent nucleoside transporter domain protein; nucleoside recognition domain protein; Na+ dependent nucleoside transporter; KEGG: sfv:SFV_2450 permease of transport system for 3 nucleosides; nucleoside transporter complement(1347774..1348976) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157916 YP_003035531.1 CDS ECBD_1281 NC_012947.1 1349312 1350550 D TIGRFAM: Mn2+/Fe2+ transporter NRAMP family; PFAM: natural resistance-associated macrophage protein; KEGG: sbc:SbBS512_E2760 manganese transporter MntH; manganese transporter MntH 1349312..1350550 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157917 YP_003035532.1 CDS ECBD_1282 NC_012947.1 1350691 1351017 R KEGG: ssn:SSON_2482 hypothetical protein; hypothetical protein complement(1350691..1351017) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157918 YP_003035533.1 CDS ECBD_1283 NC_012947.1 1351132 1352388 R PFAM: chloride channel core; KEGG: sbc:SbBS512_E2758 chloride transporter chloride channel (ClC) family; hypothetical protein complement(1351132..1352388) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157919 YP_003035534.1 CDS glk NC_012947.1 1352592 1353557 D catalyzes the conversion of ATP and D-glucose to ADP and D-glucose 6-phosphate; glucokinase 1352592..1353557 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157920 YP_003035535.1 CDS ECBD_1285 NC_012947.1 1353776 1354102 D TIGRFAM: PTS system, fructose-specific, IIB subunnit; PFAM: phosphotransferase system PTS fructose-specific IIB subunit; KEGG: ssn:SSON_2479 PTS system enzyme IIB component; PTS system fructose-specific transporter subunit IIB 1353776..1354102 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157921 YP_003035536.1 CDS ECBD_1286 NC_012947.1 1354124 1355371 D TIGRFAM: PTS system, fructose subfamily, IIC subunit; PFAM: phosphotransferase system EIIC; KEGG: sbc:SbBS512_E2755 PTS system, fructose-like, IIC component; PTS system, fructose subfamily, IIC subunit 1354124..1355371 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157922 YP_003035537.1 CDS ECBD_1287 NC_012947.1 1355386 1356471 D Xaa-Pro aminopeptidase; limited methionine aminopeptidase; aminopeptidase 1355386..1356471 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157923 YP_003035538.1 CDS ECBD_1288 NC_012947.1 1356471 1357508 D metalloprotein; exoaminopeptidase 1356471..1357508 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157924 YP_003035539.1 CDS ECBD_1289 NC_012947.1 1357533 1360028 D TIGRFAM: phosphoenolpyruvate-protein phosphotransferase; PTS system, fructose subfamily, IIsubunit alpha; PFAM: PEP-utilizing protein; PEP-utilising protein mobile region; PEP-utilising protein domain protein; phosphoryl transfer system HPr; phosphoenolpyruvate-dependent sugar phosphotransferase system EIIA 2; KEGG: sfv:SFV_2441 PTS system enzyme IIA component, enzyme I; phosphoenolpyruvate-protein phosphotransferase 1357533..1360028 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157925 YP_003035540.1 CDS ECBD_1290 NC_012947.1 1360031 1360888 R PFAM: helix-turn-helix- domain containing protein AraC type; SMART: helix-turn-helix- domain containing protein AraC type; KEGG: ssn:SSON_2474 AraC-type regulatory protein; AraC family transcriptional regulator complement(1360031..1360888) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157926 YP_003035541.1 CDS ECBD_1291 NC_012947.1 1360901 1361635 R PFAM: response regulator receiver; LytTr DNA-binding region; SMART: response regulator receiver; KEGG: sfx:S2585 2-component transcriptional regulator; two component transcriptional regulator, LytTR family complement(1360901..1361635) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157927 YP_003035542.1 CDS ECBD_1292 NC_012947.1 1361650 1363347 R KEGG: sbo:SBO_2406 sensor protein; PFAM: histidine kinase internal region; GAF domain protein; 5TM Receptors of the LytS-YhcK type transmembrane region; ATP-binding region ATPase domain protein; SMART: ATP-binding region ATPase domain protein; signal transduction histidine kinase, LytS complement(1361650..1363347) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157928 YP_003035543.1 CDS ECBD_1293 NC_012947.1 1363724 1364962 D PFAM: aminotransferase class I and II; aminotransferase 1363724..1364962 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157929 YP_003035544.1 CDS ECBD_1294 NC_012947.1 1365454 1366374 R acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(palmitoleoyl)-lipid IVA in cells subjected to cold shock; lipid A biosynthesis palmitoleoyl acyltransferase complement(1365454..1366374) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157930 YP_003035545.1 CDS ECBD_1295 NC_012947.1 1366727 1366969 D KEGG: ssn:SSON_2468 hypothetical protein; hypothetical protein 1366727..1366969 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157931 YP_003035546.1 CDS ECBD_1296 NC_012947.1 1367046 1367321 R KEGG: ssn:SSON_2467 hypothetical protein; hypothetical protein complement(1367046..1367321) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157932 YP_003035547.1 CDS ECBD_1297 NC_012947.1 1367617 1368249 D KEGG: sdy:SDY_2573 hypothetical protein; hypothetical protein 1367617..1368249 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157933 YP_003035548.1 CDS ECBD_1298 NC_012947.1 1368762 1370012 D catalyzes the formation of oxalyl-CoA from oxalate and Formyl-CoA; formyl-coenzyme A transferase 1368762..1370012 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157934 YP_003035549.1 CDS ECBD_1299 NC_012947.1 1370066 1371760 D catalyzes the formation of formyl-CoA from oxalyl-CoA; oxalyl-CoA decarboxylase 1370066..1371760 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157935 YP_003035550.1 CDS ECBD_1300 NC_012947.1 1371830 1372774 D member of the AEC family of auxin efflux transporters; unknown function; transporter YfdV 1371830..1372774 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157936 YP_003035551.1 CDS ECBD_1301 NC_012947.1 1372848 1373993 D PFAM: L-carnitine dehydratase/bile acid-inducible protein F; KEGG: sbo:SBO_2397 hypothetical protein; hypothetical protein 1372848..1373993 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157937 YP_003035552.1 CDS ECBD_1302 NC_012947.1 1374049 1377642 R PFAM: response regulator receiver; extracellular solute-binding protein family 3; Hpt domain protein; ATP-binding region ATPase domain protein; histidine kinase A domain protein; SMART: response regulator receiver; histidine kinase A domain protein; Hpt domain protein; extracellular solute-binding protein family 3; ATP-binding region ATPase domain protein; KEGG: sfv:SFV_2429 hybrid sensory histidine kinase in two-component regulatory system with EvgA; hybrid sensory histidine kinase in two-component regulatory system with EvgA complement(1374049..1377642) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157938 YP_003035553.1 CDS ECBD_1303 NC_012947.1 1377647 1378261 R response regulator in two-component regulatory system with EvgS; phosphorylated EvgA activates ompC, emrKY, gadABC, hdeAB and others genes; involved in acid resistance, osmotic adaption, and drug resistance; EvgA family transcriptional regulator complement(1377647..1378261) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157939 YP_003035554.1 CDS ECBD_1304 NC_012947.1 1378677 1379840 D TIGRFAM: efflux pump membrane protein; PFAM: secretion protein HlyD family protein; KEGG: sbc:SbBS512_E2737 drug resistance MFS transporter membrane fusion protein (MFP) subunit EmrK; efflux pump membrane protein 1378677..1379840 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157940 YP_003035555.1 CDS ECBD_1305 NC_012947.1 1379840 1381378 D TIGRFAM: drug resistance transporter EmrB/QacA subfamily; PFAM: major facilitator superfamily MFS_1; KEGG: sbo:SBO_2393 multidrug resistance protein Y; drug resistance transporter EmrB/QacA subfamily 1379840..1381378 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157941 YP_003035556.1 CDS ECBD_1306 NC_012947.1 1381486 1382814 R catalyzes the formation of pyruvate from serine; D-serine dehydratase complement(1381486..1382814) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157942 YP_003035557.1 CDS ECBD_1307 NC_012947.1 1382832 1384169 R member of the Gnt family of gluconate transporters; not involved in gluconate transport; unknown function; permease DsdX complement(1382832..1384169) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157943 YP_003035558.1 CDS ECBD_1308 NC_012947.1 1384387 1385322 D regulates the synthesis and expression of the dsdXA operon and dadA gene; DNA-binding transcriptional regulator DsdC 1384387..1385322 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157944 YP_003035559.1 CDS ECBD_1309 NC_012947.1 1385568 1386500 R PFAM: formate/nitrite transporter; KEGG: sdy:SDY_2551 transport; formate/nitrite transporter complement(1385568..1386500) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157946 YP_003035560.1 CDS ECBD_1310 NC_012947.1 1386598 1386768 D KEGG: cko:CKO_00445 hypothetical protein; hypothetical protein 1386598..1386768 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157947 YP_003035561.1 CDS ECBD_1311 NC_012947.1 1386794 1387549 D PFAM: VacJ family lipoprotein; KEGG: sdy:SDY_2550 lipoprotein precursor; VacJ family lipoprotein 1386794..1387549 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157948 YP_003035562.1 CDS ECBD_1313 NC_012947.1 1388089 1388364 D PFAM: Insertion element protein; KEGG: hdu:HD1971 insertion element IS1 1/2/3/5/6 protein InsA; Insertion element protein 1388089..1388364 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157950 YP_003035563.1 CDS ECBD_1314 NC_012947.1 1388283 1388786 D PFAM: IS1 transposase; IS1 transposase 1388283..1388786 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157951 YP_003035564.1 CDS ECBD_1316 NC_012947.1 1389933 1391273 R PFAM: membrane protein involved in aromatic hydrocarbon degradation; KEGG: ssn:SSON_2400 long-chain fatty acid outer membrane transporter; long-chain fatty acid outer membrane transporter complement(1389933..1391273) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157953 YP_003035565.1 CDS ECBD_1317 NC_012947.1 1391645 1391929 D PFAM: conserved hypothetical protein; KEGG: elf:LF82_3071 UPF0381 protein YfcZ; hypothetical protein 1391645..1391929 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157954 YP_003035566.1 CDS fadI NC_012947.1 1392110 1393420 D FadI; fatty acid oxidation complex component beta; functions in a heterotetramer with FadJ; similar to FadA2B2 complex; functions in beta-oxidation of fatty acids during anaerobic growth; 3-ketoacyl-CoA thiolase 1392110..1393420 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157955 YP_003035567.1 CDS fadJ NC_012947.1 1393420 1395564 D multifunctional enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase/3-hydroxybutyryl-CoA epimerase; catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA; exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities: forms a heterotetramer with FadI; similar to FadA2B2 complex; involved in the anaerobic degradation of long and medium-chain fatty acids in the presence of nitrate; multifunctional fatty acid oxidation complex subunit alpha 1393420..1395564 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157956 YP_003035568.1 CDS ECBD_1320 NC_012947.1 1395767 1396252 D TIGRFAM: phosphohistidine phosphatase SixA; PFAM: phosphoglycerate mutase; KEGG: ssn:SSON_2396 phosphohistidine phosphatase; phosphohistidine phosphatase 1395767..1396252 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157957 YP_003035569.1 CDS ECBD_1321 NC_012947.1 1396932 1397495 D PFAM: fimbrial protein; KEGG: sfx:S2550 fimbrial-like protein; fimbrial protein 1396932..1397495 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157958 YP_003035570.1 CDS ECBD_1322 NC_012947.1 1397578 1400223 D PFAM: fimbrial biogenesis outer membrane usher protein; KEGG: ssn:SSON_2394 PapC-like porin protein; fimbrial biogenesis outer membrane usher protein 1397578..1400223 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157959 YP_003035571.1 CDS ECBD_1323 NC_012947.1 1400243 1400995 D PFAM: Pili assembly chaperone, N-terminal; KEGG: sfv:SFV_2405 chaperone; Pili assembly chaperone, N-terminal 1400243..1400995 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157960 YP_003035572.1 CDS ECBD_1324 NC_012947.1 1401012 1401518 D KEGG: elf:LF82_p369 hypothetical protein YfcR; hypothetical protein 1401012..1401518 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157961 YP_003035573.1 CDS ECBD_1325 NC_012947.1 1401515 1401994 D PFAM: fimbrial protein; KEGG: sdy:SDY_2533 hypothetical protein; fimbrial protein 1401515..1401994 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157962 YP_003035574.1 CDS ECBD_1326 NC_012947.1 1401991 1402542 D PFAM: fimbrial protein; KEGG: elf:LF82_p367 hypothetical fimbrial-like protein YfcP; fimbrial protein 1401991..1402542 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157963 YP_003035575.1 CDS ECBD_1327 NC_012947.1 1402544 1403368 D KEGG: sfv:SFV_2401 hypothetical protein; hypothetical protein 1402544..1403368 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157964 YP_003035576.1 CDS ECBD_1328 NC_012947.1 1403494 1404045 R PFAM: Smr protein/MutS2; SMART: Smr protein/MutS2; KEGG: elf:LF82_3070 UPF0115 protein YfcN; hypothetical protein complement(1403494..1404045) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157965 YP_003035577.1 CDS ECBD_1329 NC_012947.1 1404211 1405143 D involved in methylation of ribosomal protein L3; N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase 1404211..1405143 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157966 YP_003035578.1 CDS ECBD_1330 NC_012947.1 1405178 1406263 D catalyzes the formation of chorismate from 5-O-(1-carboxyvinyl)-3-phosphoshikimate in aromatic amino acid biosynthesis; chorismate synthase 1405178..1406263 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157967 YP_003035579.1 CDS mepA NC_012947.1 1406267 1407091 D D-alanyl-D-alanine endopeptidase; functions in hydrolyzing cell wall peptidoglycan; similar to LAS metallopeptidases; forms a dimer in periplasm; penicillin-insensitive murein endopeptidase 1406267..1407091 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157968 YP_003035580.1 CDS ECBD_1332 NC_012947.1 1407091 1407900 D PFAM: protein of unknown function DUF81; KEGG: sbc:SbBS512_E2705 hypothetical protein; hypothetical protein 1407091..1407900 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157969 YP_003035581.1 CDS ECBD_1333 NC_012947.1 1407900 1408448 D PFAM: protein of unknown function DUF462; KEGG: ssn:SSON_2384 transporting ATPase; hypothetical protein 1407900..1408448 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157970 YP_003035582.1 CDS ECBD_1334 NC_012947.1 1408482 1408760 D PFAM: YfcL protein; KEGG: sdy:SDY_2524 hypothetical protein; YfcL protein 1408482..1408760 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157971 YP_003035583.1 CDS mnmC NC_012947.1 1408881 1410887 R catalyzes the formation of 5-methylaminomethyl-2-thiouridine in position 34 of the anticodon of tRNA molecules; 5-methylaminomethyl-2-thiouridine methyltransferase complement(1408881..1410887) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157972 YP_003035584.1 CDS ECBD_1336 NC_012947.1 1411046 1412266 D FabB, beta-Ketoacyl-ACP synthase I, KASI; catalyzes a condensation reaction in fatty acid biosynthesis: addition of an acyl acceptor of two carbons from malonyl-ACP; required for the elongation of short-chain unsaturated acyl-ACP; 3-oxoacyl-(acyl carrier protein) synthase I 1411046..1412266 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157973 YP_003035585.1 CDS ECBD_1337 NC_012947.1 1412531 1413709 D PFAM: major facilitator superfamily MFS_1; KEGG: sfx:S2533 hypothetical protein; hypothetical protein 1412531..1413709 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157974 YP_003035586.1 CDS flk NC_012947.1 1413706 1414701 R in Salmonella typhimurium Flk regulates flagellar biosynthesis by inhibition of the flagellar-associated type III secretion system; lowers secretion levels of the anti-sigma28 factor FlgM to the periplasm; inner membrane-anchored protein; flagella biosynthesis regulator complement(1413706..1414701) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157975 YP_003035587.1 CDS ECBD_1339 NC_012947.1 1414800 1415936 D PFAM: D-isomer specific 2-hydroxyacid dehydrogenase catalytic region; D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding; KEGG: sbc:SbBS512_E2698 erythronate-4-phosphate dehydrogenase; D-isomer specific 2-hydroxyacid dehydrogenase catalytic region 1414800..1415936 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157976 YP_003035588.1 CDS ECBD_1340 NC_012947.1 1416002 1417015 D PFAM: semialdehyde dehydrogenase dimerisation region; semialdehyde dehydrogenase NAD - binding; KEGG: ssn:SSON_2377 hypothetical protein; semialdehyde dehydrogenase 1416002..1417015 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157977 YP_003035589.1 CDS truA NC_012947.1 1417015 1417827 D mediates pseudouridylation (positions 38, 39, 40) at the tRNA anticodon region which contributes to the structural stability; tRNA pseudouridine synthase A 1417015..1417827 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157978 YP_003035590.1 CDS ECBD_1342 NC_012947.1 1417910 1418569 D PFAM: SNARE associated Golgi protein; KEGG: sfv:SFV_2386 hypothetical protein; hypothetical protein 1417910..1418569 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157979 YP_003035591.1 CDS ECBD_1343 NC_012947.1 1418725 1419639 D catalyzes the carboxylation of acetyl-CoA to malonyl-CoA; forms a tetramer of AccA2D2 subunits; acetyl-CoA carboxylase subunit beta 1418725..1419639 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157980 YP_003035592.1 CDS ECBD_1344 NC_012947.1 1419709 1420977 D TIGRFAM: FolC bifunctional protein; PFAM: Mur ligase middle domain protein; cytoplasmic peptidoglycan synthetase domain protein; KEGG: sfv:SFV_2384 bifunctional folylpolyglutamate synthase/dihydrofolate synthase; bifunctional folylpolyglutamate synthase/ dihydrofolate synthase 1419709..1420977 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157981 YP_003035593.1 CDS ECBD_1345 NC_012947.1 1420967 1421629 D PFAM: sporulation domain protein; KEGG: ssn:SSON_2372 hypothetical protein; hypothetical protein 1420967..1421629 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157982 YP_003035594.1 CDS ECBD_1346 NC_012947.1 1421888 1422376 D membrane protein required for colicin V production; colicin V production protein 1421888..1422376 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157983 YP_003035595.1 CDS ECBD_1347 NC_012947.1 1422413 1423930 D Catalyzes first step of the de novo purine nucleotide biosynthetic pathway; amidophosphoribosyltransferase 1422413..1423930 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157984 YP_003035596.1 CDS ECBD_1348 NC_012947.1 1424025 1424594 D catalyzes the formation of 2-octaprenylphenol from 3-octaprenyl-4-hydroxybenzoate; 3-octaprenyl-4-hydroxybenzoate carboxy-lyase 1424025..1424594 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157985 YP_003035597.1 CDS ECBD_1349 NC_012947.1 1424860 1425642 D KEGG: sbc:SbBS512_E2688 lysine-arginine-ornithine-binding periplasmic protein; TIGRFAM: cationic amino acid ABC transporter periplasmic binding protein; PFAM: extracellular solute-binding protein family 3; SMART: extracellular solute-binding protein family 3; cationic amino acid ABC transporter periplasmic binding protein 1424860..1425642 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157986 YP_003035598.1 CDS ECBD_1350 NC_012947.1 1425863 1426645 D KEGG: ssn:SSON_2367 histidine-binding periplasmic protein of high-affinity histidine transporter; TIGRFAM: cationic amino acid ABC transporter periplasmic binding protein; PFAM: extracellular solute-binding protein family 3; SMART: extracellular solute-binding protein family 3; cationic amino acid ABC transporter periplasmic binding protein 1425863..1426645 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157987 YP_003035599.1 CDS ECBD_1351 NC_012947.1 1426735 1427421 D TIGRFAM: polar amino acid ABC transporter inner membrane subunit; PFAM: binding-protein-dependent transporters inner membrane component; KEGG: sdy:SDY_2507 histidine transporter permease; polar amino acid ABC transporter permease 1426735..1427421 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157988 YP_003035600.1 CDS ECBD_1352 NC_012947.1 1427418 1428134 D TIGRFAM: polar amino acid ABC transporter inner membrane subunit; PFAM: binding-protein-dependent transporters inner membrane component; KEGG: sdy:SDY_2506 histidine transport, membrane protein M; polar amino acid ABC transporter permease 1427418..1428134 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158579 YP_003035601.1 CDS ECBD_1353 NC_012947.1 1428142 1428915 D PFAM: ABC transporter; SMART: ATPase AAA; KEGG: sbc:SbBS512_E2684 histidine ABC transporter ATP-binding protein; histidine/lysine/arginine/ornithine transporter subunit 1428142..1428915 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156257 YP_003035602.1 CDS ECBD_1354 NC_012947.1 1429112 1430002 D PFAM: transposase YhgA family protein; KEGG: sfv:SFV_2372 hypothetical protein; transposase YhgA family protein 1429112..1430002 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156258 YP_003035603.1 CDS ECBD_1355 NC_012947.1 1430050 1430943 R PFAM: hypothetical protein; Male sterility domain; NAD-dependent epimerase/dehydratase; KEGG: sbo:SBO_2339 sugar nucleotide epimerase; hypothetical protein complement(1430050..1430943) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156259 YP_003035604.1 CDS folX NC_012947.1 1430964 1431326 R catalyzes the formation of dihydromonapterin triphosphate from dihydroneopterin triphosphate; D-erythro-7,8-dihydroneopterin triphosphate 2'-epimerase complement(1430964..1431326) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156260 YP_003035605.1 CDS ECBD_1357 NC_012947.1 1431383 1432030 R PFAM: glutathione S-transferase domain; KEGG: sbc:SbBS512_E2678 glutathione S-transferase; glutathione S-transferase complement(1431383..1432030) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156261 YP_003035606.1 CDS ECBD_1358 NC_012947.1 1432166 1432810 D PFAM: glutathione S-transferase domain; KEGG: sbc:SbBS512_E2677 glutathione S-transferase domain protein; glutathione S-transferase domain protein 1432166..1432810 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156262 YP_003035607.1 CDS ECBD_1359 NC_012947.1 1432866 1433417 D TIGRFAM: phosphodiesterase, MJ0936 family; PFAM: metallophosphoesterase; KEGG: ssn:SSON_2357 phosphodiesterase; phosphodiesterase 1432866..1433417 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156263 YP_003035608.1 CDS ECBD_1360 NC_012947.1 1433475 1434017 D PFAM: NUDIX hydrolase; KEGG: sbc:SbBS512_E2675 hydrolase, NUDIX family protein; NUDIX hydrolase 1433475..1434017 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156264 YP_003035609.1 CDS ECBD_1361 NC_012947.1 1434050 1435591 R PFAM: C4-dicarboxylate anaerobic carrier; KEGG: sbc:SbBS512_E2674 C4-dicarboxylate anaerobic carrier protein; hypothetical protein complement(1434050..1435591) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156265 YP_003035610.1 CDS ECBD_1362 NC_012947.1 1435760 1437904 R catalyzes the synthesis of acetylphosphate or propionylphosphate from acetyl-CoA or propionyl-CoA and inorganic phosphate; when using propionyl-CoA the enzyme is functioning in the anaerobic pathway catabolizing threonine to propionate; phosphate acetyltransferase complement(1435760..1437904) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156266 YP_003035611.1 CDS ECBD_1363 NC_012947.1 1437979 1439181 R AckA utilizes acetate and can acetylate CheY which increases signal strength during flagellar rotation; utilizes magnesium and ATP; also involved in conversion of acetate to aceyl-CoA; acetate kinase complement(1437979..1439181) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156267 YP_003035612.1 CDS ECBD_1364 NC_012947.1 1439520 1439975 D PFAM: protein of unknown function DUF412; KEGG: sdy:SDY_2491 hypothetical protein; hypothetical protein 1439520..1439975 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156268 YP_003035613.1 CDS ECBD_1365 NC_012947.1 1440058 1440552 D PFAM: YfbU family protein; KEGG: ssn:SSON_2351 hypothetical protein; hypothetical protein 1440058..1440552 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156269 YP_003035614.1 CDS ECBD_1366 NC_012947.1 1440563 1441213 D TIGRFAM: HAD-superfamily hydrolase, subfamily IA, variant 3; HAD-superfamily hydrolase, subfamily IA, variant 1; PFAM: Haloacid dehalogenase domain protein hydrolase; KEGG: elf:LF82_3055 phosphatase YfbT; phosphatase 1440563..1441213 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156270 YP_003035615.1 CDS ECBD_1367 NC_012947.1 1441300 1443132 D PFAM: citrate transporter; TrkA-C domain protein; TRAP C4-dicarboxylate transporter permease DctM subunit; KEGG: ssn:SSON_2349 transporter; citrate transporter 1441300..1443132 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156271 YP_003035616.1 CDS ECBD_1368 NC_012947.1 1443191 1443790 R PFAM: metal-dependent phosphohydrolase HD sub domain; SMART: metal-dependent phosphohydrolase HD region; hypothetical protein complement(1443191..1443790) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156272 YP_003035617.1 CDS ECBD_1369 NC_012947.1 1443874 1445091 R broad specificity; family IV; in Corynebacterium glutamicum this protein can use glutamate, 2-aminobutyrate, and aspartate as amino donors and pyruvate as the acceptor; aminotransferase AlaT complement(1443874..1445091) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156273 YP_003035618.1 CDS ECBD_1370 NC_012947.1 1445517 1445771 R hypothetical protein complement(1445517..1445771) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156274 YP_003035619.1 CDS ECBD_1371 NC_012947.1 1446011 1446949 D PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: elf:LF82_1233 HTH-type transcriptional regulator LrhA; LysR family transcriptional regulator 1446011..1446949 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156275 YP_003035620.1 CDS ECBD_1372 NC_012947.1 1447153 1447317 R hypothetical protein complement(1447153..1447317) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156276 YP_003035621.1 CDS ECBD_1373 NC_012947.1 1447580 1448023 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit A 1447580..1448023 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156277 YP_003035622.1 CDS ECBD_1374 NC_012947.1 1448039 1448701 D The point of entry for the majority of electrons that traverse the respiratory chain eventually resulting in the reduction of oxygen; NADH dehydrogenase subunit B 1448039..1448701 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156278 YP_003035623.1 CDS ECBD_1375 NC_012947.1 1448795 1450597 D NuoCD; NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain; subunits NuoCD, E, F, and G constitute the peripheral sector of the complex; in Escherichia coli this gene encodes a fusion protein of NuoC and NuoD that are found separate in other organisms; bifunctional NADH:ubiquinone oxidoreductase subunit C/D 1448795..1450597 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156279 YP_003035624.1 CDS ECBD_1376 NC_012947.1 1450600 1451100 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit E 1450600..1451100 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156280 YP_003035625.1 CDS ECBD_1377 NC_012947.1 1451097 1452434 D shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain; NuoF is part of the soluble NADH dehydrogenase fragment, which represents the electron input part of NADH dehydrogenase; NADH dehydrogenase I subunit F 1451097..1452434 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156281 YP_003035626.1 CDS ECBD_1378 NC_012947.1 1452487 1455213 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit G 1452487..1455213 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156282 YP_003035627.1 CDS ECBD_1379 NC_012947.1 1455210 1456187 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit H 1455210..1456187 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156283 YP_003035628.1 CDS ECBD_1380 NC_012947.1 1456202 1456744 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit I 1456202..1456744 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156284 YP_003035629.1 CDS ECBD_1381 NC_012947.1 1456756 1457310 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit J 1456756..1457310 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156285 YP_003035630.1 CDS ECBD_1382 NC_012947.1 1457307 1457609 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit K 1457307..1457609 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156286 YP_003035631.1 CDS ECBD_1383 NC_012947.1 1457606 1459447 D Catalyzes the transfer of electrons from NADH to ubiquinone; NADH dehydrogenase subunit L 1457606..1459447 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156287 YP_003035632.1 CDS ECBD_1384 NC_012947.1 1459678 1461207 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit M 1459678..1461207 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156288 YP_003035633.1 CDS ECBD_1385 NC_012947.1 1461214 1462671 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit N 1461214..1462671 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156289 YP_003035634.1 CDS ECBD_1386 NC_012947.1 1462739 1463578 R PFAM: Integrase catalytic region; KEGG: bcj:BCAS0612 integrase; Integrase catalytic region complement(1462739..1463578) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156290 YP_003035635.1 CDS ECBD_1387 NC_012947.1 1463575 1464096 R KEGG: pnu:Pnuc_1431 ISPsy8, transposase OrfA; ISPsy8, transposase OrfA complement(1463575..1464096) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156291 YP_003035636.1 CDS ECBD_1388 NC_012947.1 1464183 1464686 R PFAM: Domain of unknown function DUF1877; KEGG: sdy:SDY_2471 hypothetical protein; hypothetical protein complement(1464183..1464686) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156292 YP_003035637.1 CDS ECBD_1389 NC_012947.1 1465806 1467014 R ElaD; specific cysteine protease which targets ubiquitin and ubiquitin-like proteins covalently bound to target proteins; SseL in Salmonella is required for macrophage killing and virulence, and it is secreted by the salmonella pathogenicity island 2 type III secretion system; deubiquitinase complement(1465806..1467014) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156293 YP_003035638.1 CDS ECBD_1390 NC_012947.1 1467205 1468122 R member of metallo-beta-lactamase family; the purified enzyme from Escherichia coli forms dimeric zinc phosphodiesterase; in Bacillus subtilis this protein is a 3'-tRNA processing endoribonuclease and is essential while in Escherichia coli it is not; associates with two zinc ions; ribonuclease Z complement(1467205..1468122) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156294 YP_003035639.1 CDS ECBD_1391 NC_012947.1 1468187 1468648 D PFAM: GCN5-related N-acetyltransferase; KEGG: sfv:SFV_2338 hypothetical protein; hypothetical protein 1468187..1468648 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156295 YP_003035640.1 CDS ECBD_1392 NC_012947.1 1468703 1469008 D PFAM: protein of unknown function DUF883 ElaB; KEGG: sdy:SDY_2462 hypothetical protein; hypothetical protein 1468703..1469008 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156296 YP_003035641.1 CDS ECBD_1393 NC_012947.1 1469087 1470382 D TIGRFAM: isochorismate synthase; PFAM: chorismate binding-like; KEGG: sfx:S2478 menaquinone-specific isochorismate synthase; isochorismate synthase 1469087..1470382 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156297 YP_003035642.1 CDS ECBD_1394 NC_012947.1 1470471 1472141 D SEPHCHC synthase; forms 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1- carboxylate from 2-oxoglutarate and isochorismate in menaquinone biosynthesis; 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase 1470471..1472141 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156298 YP_003035643.1 CDS ECBD_1395 NC_012947.1 1472138 1472896 D catalyzes the hydrolysis of the thioester bond in palmitoyl-CoA; acyl-CoA thioester hydrolase YfbB 1472138..1472896 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156299 YP_003035644.1 CDS ECBD_1396 NC_012947.1 1472911 1473768 D catalyzes the formation of 1,4-dihydroxy-2-naphthoate from O-succinylbenzoyl-CoA; naphthoate synthase 1472911..1473768 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156300 YP_003035645.1 CDS ECBD_1397 NC_012947.1 1473768 1474730 D catalyzes the dehydration of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid to form O-succinylbenzoate; O-succinylbenzoate synthase 1473768..1474730 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156301 YP_003035646.1 CDS ECBD_1398 NC_012947.1 1474727 1476082 D TIGRFAM: O-succinylbenzoate-CoA ligase; PFAM: AMP-dependent synthetase and ligase; KEGG: sdy:SDY_2456 O-succinylbenzoic acid--CoA ligase; O-succinylbenzoic acid--CoA ligase 1474727..1476082 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156302 YP_003035647.1 CDS ECBD_1399 NC_012947.1 1476192 1476458 D KEGG: ssn:SSON_2320 polymyxin resistance protein B; polymyxin resistance protein B 1476192..1476458 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156303 YP_003035648.1 CDS ECBD_1400 NC_012947.1 1476452 1476838 R KEGG: ssn:SSON_2319 hypothetical protein; hypothetical protein complement(1476452..1476838) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156304 YP_003035649.1 CDS ECBD_1401 NC_012947.1 1476838 1477173 R PFAM: small multidrug resistance protein; KEGG: sbc:SbBS512_E2634 multidrug resistance protein, SMR family; small multidrug resistance protein complement(1476838..1477173) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156305 YP_003035650.1 CDS arnT NC_012947.1 1477170 1478822 R catalyzes the addition of 4-amino-4-deoxy-L-arabinose to lipid A; 4-amino-4-deoxy-L-arabinose transferase complement(1477170..1478822) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156306 YP_003035651.1 CDS ECBD_1403 NC_012947.1 1478822 1479712 R PFAM: polysaccharide deacetylase; KEGG: ssn:SSON_2317 hypothetical protein; polysaccharide deacetylase complement(1478822..1479712) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156307 YP_003035652.1 CDS ECBD_1404 NC_012947.1 1479709 1481691 R Bifunctional polymyxin resistance arnA protein; catalyzes the decarboxylation of UDP-glucuronic acid to UDP-4-keto-arabinose and the addition of a formyl group to UDP-4-amino-4-deoxy-L-arabinose to form UDP-L-4-formamido-arabinose; active in certain mutant strains; bifunctional UDP-glucuronic acid decarboxylase/UDP-4-amino-4-deoxy-L-arabinose formyltransferase complement(1479709..1481691) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156308 YP_003035653.1 CDS ECBD_1405 NC_012947.1 1481691 1482659 R catalyzes the transfer of 4-deoxy-4-formamido-L-arabinose from UDP to undecaprenyl phosphate; UDP phosphate 4-deoxy-4-formamido-L-arabinose transferase complement(1481691..1482659) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156309 YP_003035654.1 CDS ECBD_1406 NC_012947.1 1482663 1483802 R catalyzes the conversion of UDP-4-keto-arabinose to UDP-4-amino-4-deoxy-L-arabinose; UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase complement(1482663..1483802) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156310 YP_003035655.1 CDS ECBD_1407 NC_012947.1 1484110 1484712 D PFAM: phosphoglycerate mutase; KEGG: ssn:SSON_2313 protein induced by aluminum; phosphoglycerate mutase 1484110..1484712 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156311 YP_003035656.1 CDS ECBD_1408 NC_012947.1 1484751 1485176 R PFAM: NUDIX hydrolase; KEGG: elf:LF82_1535 NUDIX hydrolase YfaO; NUDIX hydrolase complement(1484751..1485176) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156312 YP_003035657.1 CDS ECBD_1409 NC_012947.1 1485455 1485997 D PFAM: YfaZ family protein; YfaZ family protein 1485455..1485997 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156313 YP_003035658.1 CDS ECBD_1410 NC_012947.1 1486097 1487299 D TIGRFAM: competence/damage-inducible protein CinA; molybdenum cofactor synthesis protein; PFAM: molybdopterin binding domain; KEGG: sfx:S2461 competence damage-inducible protein A; competence damage-inducible protein A 1486097..1487299 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156314 YP_003035659.1 CDS ECBD_1411 NC_012947.1 1487519 1488301 D PFAM: transcriptional regulator IclR ; regulatory protein IclR; SMART: regulatory protein IclR; KEGG: ssn:SSON_2309 regulator; transcriptional regulator, IclR family 1487519..1488301 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156315 YP_003035660.1 CDS ECBD_1412 NC_012947.1 1488316 1489521 D PFAM: mandelate racemase/muconate lactonizing protein; KEGG: sbo:SBO_2047 racemase; mandelate racemase/muconate lactonizing protein 1488316..1489521 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156316 YP_003035661.1 CDS ECBD_1413 NC_012947.1 1489578 1490867 D PFAM: major facilitator superfamily MFS_1; KEGG: sbo:SBO_2048 transporter; major facilitator superfamily MFS_1 1489578..1490867 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156317 YP_003035662.1 CDS ECBD_1414 NC_012947.1 1490885 1491688 D PFAM: HpcH/HpaI aldolase; KEGG: elf:LF82_3041 uncharacterized protein YfaU; aldolase 1490885..1491688 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156318 YP_003035663.1 CDS ECBD_1415 NC_012947.1 1491729 1492631 R PFAM: transposase YhgA family protein; KEGG: sbc:SbBS512_E2627 hypothetical protein; hypothetical protein complement(1491729..1492631) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156319 YP_003035664.1 CDS ECBD_1416 NC_012947.1 1492674 1492799 R KEGG: sbc:SbBS512_E2626 hypothetical protein; hypothetical protein complement(1492674..1492799) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156320 YP_003035665.1 CDS glpC NC_012947.1 1492824 1494014 R anaerobic; with GlpAB catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate; sn-glycerol-3-phosphate dehydrogenase subunit C complement(1492824..1494014) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156321 YP_003035666.1 CDS ECBD_1418 NC_012947.1 1494011 1495270 R sn-glycerol-3-phosphate dehydrogenase (anaerobic); catalyzes the formation of dihydroxyacetone from glycerol 3-phosphate; part of GlpABC complex; presumably this subunit is responsible for membrane interactions and contains iron-sulfur clusters; anaerobic glycerol-3-phosphate dehydrogenase subunit B complement(1494011..1495270) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156322 YP_003035667.1 CDS glpA NC_012947.1 1495260 1496888 R anaerobic, catalyzes the conversion of glycerol 3-phosphate to dihydroxyacetone using fumarate or nitrate as electron acceptor; sn-glycerol-3-phosphate dehydrogenase subunit A complement(1495260..1496888) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156323 YP_003035668.1 CDS glpT NC_012947.1 1497161 1498519 D catalyzes the uptake of glycerol-3-phosphate into the cell with the simultaneous export of inorganic phosphate from the cell; sn-glycerol-3-phosphate transporter 1497161..1498519 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156324 YP_003035669.1 CDS glpQ NC_012947.1 1498524 1499600 D hydrolyzes deacylated phospholipids to glycerol 3-phosphate and the corresponding alcohols; periplasmic; glycerophosphodiester phosphodiesterase 1498524..1499600 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156325 YP_003035670.1 CDS ECBD_1422 NC_012947.1 1499642 1499848 R KEGG: sfx:S2453 hypothetical protein; hypothetical protein complement(1499642..1499848) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156326 YP_003035671.1 CDS ECBD_1423 NC_012947.1 1500063 1500713 D PFAM: lipopolysaccharide kinase; KEGG: sfv:SFV_2310 hypothetical protein; hypothetical protein 1500063..1500713 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156327 YP_003035672.1 CDS ECBD_1424 NC_012947.1 1500767 1501021 R plays a role in maintenance and possibly the biosynthesis of diferric-tyrosyl radical cofactor, essential for nucleotide reduction catalyzed by ribonucleotide reductases; 2Fe-2S ferredoxin YfaE complement(1500767..1501021) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156328 YP_003035673.1 CDS nrdB NC_012947.1 1501021 1502151 R B2 or R2 protein; type 1a enzyme; catalyzes the rate-limiting step in dNTP synthesis; converts nucleotides to deoxynucleotides; forms a homodimer and then a multimeric complex with NrdA; ribonucleotide-diphosphate reductase subunit beta complement(1501021..1502151) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156329 YP_003035674.1 CDS ECBD_1426 NC_012947.1 1502297 1504582 R Catalyzes the rate-limiting step in dNTP synthesis; ribonucleotide-diphosphate reductase subunit alpha complement(1502297..1504582) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156330 YP_003035675.1 CDS ECBD_1427 NC_012947.1 1505326 1509030 D TIGRFAM: outer membrane autotransporter barrel domain protein; polymorphic outer membrane protein; autotransporter-associated beta strand repeat protein; PFAM: Autotransporter beta- domain protein; polymorphic membrane protein Chlamydia; Pertactin; adhesin 1505326..1509030 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156331 YP_003035676.1 CDS ECBD_1428 NC_012947.1 1509158 1509880 R Involved in ubiquinone biosynthesis; 3-demethylubiquinone-9 3-methyltransferase complement(1509158..1509880) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156332 YP_003035677.1 CDS ECBD_1429 NC_012947.1 1510027 1512654 D negatively supercoils closed circular double-stranded DNA; DNA gyrase subunit A 1510027..1512654 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156333 YP_003035678.1 CDS ECBD_1430 NC_012947.1 1512803 1514491 D KEGG: elf:LF82_3033 uncharacterized protein YfaA; hypothetical protein 1512803..1514491 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156334 YP_003035679.1 CDS ECBD_1431 NC_012947.1 1514488 1515111 D PFAM: protein of unknown function DUF1175; hypothetical protein 1514488..1515111 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156335 YP_003035680.1 CDS ECBD_1432 NC_012947.1 1515045 1519649 D PFAM: alpha-2-macroglobulin; alpha-2-macroglobulin 2; alpha-2-macroglobulin 1515045..1519649 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156336 YP_003035681.1 CDS ECBD_1433 NC_012947.1 1519650 1521299 D KEGG: ssn:SSON_2285 hypothetical protein; hypothetical protein 1519650..1521299 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157342 YP_003035682.1 CDS ECBD_1434 NC_012947.1 1521304 1522080 D KEGG: sfv:SFV_2294 hypothetical protein; hypothetical protein 1521304..1522080 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159969 YP_003035683.1 CDS ECBD_1435 NC_012947.1 1522154 1523338 R KEGG: ssn:SSON_2283 acetyl-CoA acetyltransferase; TIGRFAM: acetyl-CoA acetyltransferase; PFAM: thiolase; acetyl-CoA acetyltransferase complement(1522154..1523338) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159970 YP_003035684.1 CDS ECBD_1436 NC_012947.1 1523369 1524691 R PFAM: short chain fatty acid transporter; KEGG: elf:LF82_0189 short-chain fatty acids transporter; short chain fatty acid transporter complement(1523369..1524691) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159971 YP_003035685.1 CDS ECBD_1437 NC_012947.1 1524688 1525338 R KEGG: elf:LF82_0185 acetate CoA-transferase subunit beta; TIGRFAM: 3-oxoacid CoA-transferase, B subunit; PFAM: coenzyme A transferase; 3-oxoacid CoA-transferase, B subunit complement(1524688..1525338) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159972 YP_003035686.1 CDS ECBD_1438 NC_012947.1 1525338 1526000 R KEGG: ssn:SSON_2280 acetyl-CoA:acetoacetyl-CoA transferase subunit alpha; TIGRFAM: 3-oxoacid CoA-transferase, subunit alpha; PFAM: coenzyme A transferase; acetyl-CoA:acetoacetyl-CoA transferase subunit alpha complement(1525338..1526000) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159973 YP_003035687.1 CDS ECBD_1439 NC_012947.1 1526196 1527581 R with AtoS is a two-component regulatory system involved in the transcriptional regulation of the ato genes for acetoacetate metabolism; acetoacetate metabolism regulatory protein AtoC complement(1526196..1527581) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159974 YP_003035688.1 CDS ECBD_1440 NC_012947.1 1527578 1529404 R with AtoC is a member of a two-component regulatory system involved in the transcriptional regulation of the ato genes for acetoacetate metabolism; sensory histidine kinase AtoS complement(1527578..1529404) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159975 YP_003035689.1 CDS ECBD_1441 NC_012947.1 1529571 1532420 D PFAM: ATP-binding region ATPase domain protein; response regulator receiver; histidine kinase A domain protein; SMART: ATP-binding region ATPase domain protein; response regulator receiver; histidine kinase A domain protein; KEGG: ssn:SSON_2277 hybrid sensory kinase in two-component regulatory system with RcsB and YojN; hybrid sensory kinase in two-component regulatory system with RcsB and YojN 1529571..1532420 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159976 YP_003035690.1 CDS ECBD_1442 NC_012947.1 1532695 1532970 D PFAM: Insertion element protein; KEGG: hdu:HD1971 insertion element IS1 1/2/3/5/6 protein InsA; Insertion element protein 1532695..1532970 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159977 YP_003035691.1 CDS ECBD_1443 NC_012947.1 1532889 1533392 D PFAM: IS1 transposase; KEGG: sty:HCM1.136a IS1 transposase; IS1 transposase 1532889..1533392 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159978 YP_003035692.1 CDS ECBD_1445 NC_012947.1 1534509 1535564 D catalyzes the conversion of aminoimidazole ribotide to the 4-amino-5-hydroxymethyl-2-methyl pyrimidine moiety of thiamine; involved in the maintenance and/or assembly of sulfur clusters; periplasmic lipoporotein anchored to the inner membrane; part of the thiamine pyrophosphate biosynthesis pathway; thiamine biosynthesis lipoprotein ApbE 1534509..1535564 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159980 YP_003035693.1 CDS ECBD_1446 NC_012947.1 1535638 1536702 D KEGG: sbo:SBO_2094 O6-methylguanine-DNA methyltransferase; transcription activator/repressor; TIGRFAM: methylated-DNA/protein-cysteine methyltransferase; PFAM: methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding; Ada metal-binding domain protein; helix-turn-helix- domain containing protein AraC type; methylguanine DNA methyltransferase ribonuclease domain protein; SMART: helix-turn-helix- domain containing protein AraC type; AraC family transcriptional regulator 1535638..1536702 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159981 YP_003035694.1 CDS ECBD_1447 NC_012947.1 1536702 1537352 D TIGRFAM: alkylated DNA repair protein AlkB; PFAM: 2OG-Fe(II) oxygenase; KEGG: sbc:SbBS512_E0728 alkylated DNA repair protein AlkB; alkylated DNA repair protein AlkB 1536702..1537352 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159982 YP_003035695.1 CDS ECBD_1448 NC_012947.1 1537428 1539071 D efflux pump for the antibacterial peptide microcin J25; multidrug transporter membrane component/ATP-binding component 1537428..1539071 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159983 YP_003035696.1 CDS ECBD_1449 NC_012947.1 1539289 1540935 D malate dehydrogenase; catalyzes the oxidation of malate to oxaloacetate; malate:quinone oxidoreductase 1539289..1540935 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159984 YP_003035697.1 CDS ECBD_1450 NC_012947.1 1541765 1542253 R serine protease inhibitor, inhibits trypsin and other proteases; ecotin complement(1541765..1542253) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159985 YP_003035698.1 CDS ECBD_1451 NC_012947.1 1542388 1542552 R KEGG: sbc:SbBS512_E0732 hypothetical protein; hypothetical protein complement(1542388..1542552) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159986 YP_003035699.1 CDS ECBD_1452 NC_012947.1 1542661 1543155 D Predicted role in electron transfer to the periplasmic nitrate reductase protein NapA.; ferredoxin-type protein 1542661..1543155 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159987 YP_003035700.1 CDS ECBD_1453 NC_012947.1 1543145 1543408 D PFAM: NapD family protein; KEGG: ssn:SSON_2265 assembly protein for periplasmic nitrate reductase; assembly protein for periplasmic nitrate reductase 1543145..1543408 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159988 YP_003035701.1 CDS ECBD_1454 NC_012947.1 1543405 1545891 D periplasmic; catalytic subunit; with NapBC catalyzes the reduction of nitrate to nitrite; NapAB receives electrons from NapC; nitrate reductase catalytic subunit 1543405..1545891 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159989 YP_003035702.1 CDS napG NC_012947.1 1545898 1546593 D part of NapHG quinol dehydrogenase; couples electron transfer from ubiquinone-ubiquinol couple via NapC/B to NapA; secreted by twin arginine translocation pathway; quinol dehydrogenase periplasmic component 1545898..1546593 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159990 YP_003035703.1 CDS napH NC_012947.1 1546580 1547443 D part of NapHG quinol dehydrogenase; couples electron transfer from ubiquinone-ubiquinol couple via NapC/B to NapA; quinol dehydrogenase membrane component 1546580..1547443 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159991 YP_003035704.1 CDS napB NC_012947.1 1547440 1547889 D small subunit of periplasmic nitrate reductase; receives electrons from the membrane-bound NapC and passes them to NapA; citrate reductase cytochrome C subunit 1547440..1547889 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159992 YP_003035705.1 CDS ECBD_1458 NC_012947.1 1547899 1548501 D with NapABDFGH functions as a nitrate reductase; NapC functions as an electron shuttle between NapAB and NapGH or quinone; cytochrome C protein NapC 1547899..1548501 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159993 YP_003035706.1 CDS ECBD_1459 NC_012947.1 1548520 1549137 D ATP-binding protein; required for proper cytochrome C maturation; cytochrome C biogenesis protein CcmA 1548520..1549137 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159994 YP_003035707.1 CDS ECBD_1460 NC_012947.1 1549134 1549796 D PFAM: cytochrome C biogenesis protein CcmB; KEGG: sdy:SDY_0878 heme exporter protein B; cytochrome C biogenesis protein CcmB 1549134..1549796 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159995 YP_003035708.1 CDS ECBD_1461 NC_012947.1 1549838 1550575 D PFAM: cytochrome C assembly protein; KEGG: sdy:SDY_0879 heme exporter protein C; cytochrome C assembly protein 1549838..1550575 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159996 YP_003035709.1 CDS ECBD_1462 NC_012947.1 1550572 1550781 D PFAM: Heme exporter protein D (CcmD); KEGG: sdy:SDY_0880 heme exporter protein C; Heme exporter protein D (CcmD) 1550572..1550781 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159997 YP_003035710.1 CDS ECBD_1463 NC_012947.1 1550778 1551257 D CycJ; periplasmic heme chaperone that binds heme transiently via a histidine residue and delivers it to newly synthesized and exported c-type cytochromes; requires the ATP hydrolysis activity of the CcmA protein in order to transfer the heme to the apocytochrome; part of the cytochrome C maturation system; periplasmic protein anchored to the inner membrane; cytochrome C biogenesis protein CcmE 1550778..1551257 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159998 YP_003035711.1 CDS ECBD_1464 NC_012947.1 1551254 1553197 D PFAM: cytochrome C assembly protein; KEGG: sbo:SBO_2111 cytochrome C biogenesis protein; cytochrome C assembly protein 1551254..1553197 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159999 YP_003035712.1 CDS ECBD_1465 NC_012947.1 1553194 1553751 D PFAM: redoxin; alkyl hydroperoxide reductase/ thiol specific antioxidant/ Mal allergen; KEGG: sbc:SbBS512_E0746 thiol:disulfide interchange protein DsbE; redoxin 1553194..1553751 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160000 YP_003035713.1 CDS ECBD_1466 NC_012947.1 1553748 1554800 D PFAM: cytochrome C biogenesis protein; KEGG: sfv:SFV_2270 subunit of heme lyase; cytochrome C biogenesis protein 1553748..1554800 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160001 YP_003035714.1 CDS ECBD_1467 NC_012947.1 1555011 1555658 R nitrate/nitrite response regulator NarP; NarP is phosphorylated by NarX and NarQ and can activate fdnG and nitrite or nitrate reductase systems; represses expression of other anaerobic genes; transcriptional regulator NarP complement(1555011..1555658) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160002 YP_003035715.1 CDS ECBD_1468 NC_012947.1 1555978 1558569 D PFAM: Pertactin; Autotransporter beta- domain protein; KEGG: sfv:SFV_2268 ATP-binding component of a transport system; Pertactin 1555978..1558569 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160003 YP_003035716.1 CDS ECBD_1469 NC_012947.1 1558823 1560583 R PFAM: sulfatase; KEGG: elf:LF82_3032 inner membrane protein YejM; sulfatase complement(1558823..1560583) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160005 YP_003035717.1 CDS ECBD_1470 NC_012947.1 1560603 1560830 R PFAM: protein of unknown function DUF1414; KEGG: sbc:SbBS512_E0770 hypothetical protein; hypothetical protein complement(1560603..1560830) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160006 YP_003035718.1 CDS ECBD_1471 NC_012947.1 1561012 1562019 D PFAM: 37kDa nucleoid-associated protein; KEGG: sfv:SFV_2264 nucleoid-associated protein NdpA; nucleoid-associated protein NdpA 1561012..1562019 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160007 YP_003035719.1 CDS ECBD_1472 NC_012947.1 1562158 1562442 R the Ctc family of proteins consists of two types, one that contains the N-terminal ribosomal protein L25 domain only which in Escherichia coli binds the 5S rRNA while a subset of proteins contain a C-terminal extension that is involved in the stress response; 50S ribosomal protein L25 complement(1562158..1562442) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160008 YP_003035720.1 CDS ECBD_1473 NC_012947.1 1562567 1564327 R PFAM: type III restriction protein res subunit; helicase domain protein; DEAD/DEAH box helicase; SMART: DEAD-like helicase ; helicase; KEGG: sdy:SDY_0895 ATP-dependent helicase; type III restriction protein res subunit complement(1562567..1564327) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160009 YP_003035721.1 CDS ECBD_1474 NC_012947.1 1564476 1565171 D catalyzes the synthesis pseudouridine from uracil-516 in 16S ribosomal RNA; 16S rRNA pseudouridylate synthase A 1564476..1565171 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160010 YP_003035722.1 CDS ECBD_1475 NC_012947.1 1565199 1566389 D Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance; bicyclomycin/multidrug efflux system 1565199..1566389 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160011 YP_003035723.1 CDS ECBD_1476 NC_012947.1 1566722 1567066 D KEGG: sfv:SFV_2259 hypothetical protein; hypothetical protein 1566722..1567066 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160012 YP_003035724.1 CDS ECBD_1477 NC_012947.1 1567070 1568659 R PFAM: ABC transporter; SMART: ATPase AAA; KEGG: sdy:SDY_0899 ATP-binding component of a transporter; ABC transporter complement(1567070..1568659) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160013 YP_003035725.1 CDS ECBD_1478 NC_012947.1 1568661 1569686 R PFAM: binding-protein-dependent transporters inner membrane component; KEGG: sbo:SBO_2145 transporter permease protein; binding-protein-dependent transporters inner membrane component complement(1568661..1569686) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160014 YP_003035726.1 CDS ECBD_1479 NC_012947.1 1569686 1570780 R PFAM: binding-protein-dependent transporters inner membrane component; KEGG: ssn:SSON_2234 transporter permease protein; binding-protein-dependent transporters inner membrane component complement(1569686..1570780) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160015 YP_003035727.1 CDS ECBD_1480 NC_012947.1 1570781 1572595 R PFAM: extracellular solute-binding protein family 5; extracellular solute-binding protein family 5 complement(1570781..1572595) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160016 YP_003035728.1 CDS ECBD_1481 NC_012947.1 1572677 1574233 R PFAM: EAL domain protein; SMART: EAL domain protein; KEGG: sbc:SbBS512_E0782 cyclic diguanylate phosphodiesterase; hypothetical protein complement(1572677..1574233) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160017 YP_003035729.1 CDS spr NC_012947.1 1574414 1574980 R by similarity, Spr seems to have peptidase activity; involved in thermoresistance; outer membrane lipoprotein complement(1574414..1574980) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160018 YP_003035730.1 CDS ECBD_1483 NC_012947.1 1575392 1576105 R PFAM: phosphoesterase PA-phosphatase related; SMART: phosphoesterase PA-phosphatase related; KEGG: ssn:SSON_2230 hypothetical protein; phosphoesterase PA-phosphatase related complement(1575392..1576105) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160019 YP_003035731.1 CDS ECBD_1484 NC_012947.1 1576144 1577130 R PFAM: cobalamin synthesis protein P47K; cobalamin synthesis CobW domain protein; KEGG: sbc:SbBS512_E0785 CobW/P47K family protein; cobalamin synthesis protein P47K complement(1576144..1577130) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160020 YP_003035732.1 CDS ECBD_1485 NC_012947.1 1577248 1578714 R PFAM: Mannitol dehydrogenase domain; Mannitol dehydrogenase rossman domain; KEGG: ssn:SSON_2228 oxidoreductase; mannitol dehydrogenase complement(1577248..1578714) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160021 YP_003035733.1 CDS ECBD_1486 NC_012947.1 1578937 1579509 R Stimulates the peptidyltransferase activity of the 70S ribosome and enhances dipeptide synthesis with N-formylmethionyl-tRNA and puromycin in vitro, suggesting its involvement in the formation of the first peptide bond of a protein; elongation factor P complement(1578937..1579509) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160022 YP_003035734.1 CDS ECBD_1487 NC_012947.1 1579664 1579918 D PFAM: protein of unknown function UPF0153; KEGG: elf:LF82_3023 UPF0153 protein YeiW; hypothetical protein 1579664..1579918 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160023 YP_003035735.1 CDS ECBD_1488 NC_012947.1 1579915 1581096 R TIGRFAM: sugar efflux transporter; PFAM: major facilitator superfamily MFS_1; sugar efflux transporter complement(1579915..1581096) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160024 YP_003035736.1 CDS ECBD_1489 NC_012947.1 1581464 1582594 D phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system; catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane; IIA is phosphorylated by phospho-HP which then transfers the phosphoryl group to the IIB componentr; bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein 1581464..1582594 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160025 YP_003035737.1 CDS fruK NC_012947.1 1582594 1583532 D converts fructose-1-phosphate and ATP to fructose-1,6-bisphosphate and ADP; highly specific for fructose-1-phopshate; similar to PfkB; forms homodimers; 1-phosphofructokinase 1582594..1583532 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160026 YP_003035738.1 CDS ECBD_1491 NC_012947.1 1583549 1585240 D phosphoenolpyruvate-dependent sugar phosphotransferase system; catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane; IIB is phosphorylated by IIA and then transfers the phosphoryl group to the sugar; IIC forms the translocation channel; PTS system fructose-specific transporter subunits IIBC 1583549..1585240 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160027 YP_003035739.1 CDS ECBD_1492 NC_012947.1 1585664 1586605 D PFAM: PfkB domain protein; KEGG: sfv:SFV_2241 hypothetical protein; hypothetical protein 1585664..1586605 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160028 YP_003035740.1 CDS ECBD_1493 NC_012947.1 1586593 1587531 D PFAM: indigoidine synthase A family protein; KEGG: sfv:SFV_2240 hypothetical protein; indigoidine synthase A family protein 1586593..1587531 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160029 YP_003035741.1 CDS ECBD_1494 NC_012947.1 1587625 1588875 D TIGRFAM: nucleoside transporter; PFAM: Na+ dependent nucleoside transporter domain protein; nucleoside recognition domain protein; Na+ dependent nucleoside transporter; KEGG: sbc:SbBS512_E0801 nucleoside transporter NupC family; nucleoside transporter 1587625..1588875 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160030 YP_003035742.1 CDS ECBD_1495 NC_012947.1 1588946 1589644 R activator of nucleoside metabolism; DNA-binding transcriptional activator YeiL complement(1588946..1589644) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160031 YP_003035743.1 CDS rihB NC_012947.1 1589774 1590715 D Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively; ribonucleoside hydrolase 2 1589774..1590715 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160032 YP_003035744.1 CDS ECBD_1497 NC_012947.1 1590815 1592065 D TIGRFAM: nucleoside transporter; PFAM: Na+ dependent nucleoside transporter domain protein; nucleoside recognition domain protein; Na+ dependent nucleoside transporter; KEGG: ssn:SSON_2217 transport system permease; nucleoside transporter 1590815..1592065 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160033 YP_003035745.1 CDS ECBD_1498 NC_012947.1 1592121 1593209 R PFAM: PfkB domain protein; KEGG: sbc:SbBS512_E0806 kinase, PfkB family; hypothetical protein complement(1592121..1593209) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160034 YP_003035746.1 CDS ECBD_1499 NC_012947.1 1593212 1594069 R Assists in DNA repair by cleaving phosphodiester bonds at apurinic or apyrimidinic sties to produce new 5' ends that are base-free deoxyribose 5-phosphate residues; endonuclease IV complement(1593212..1594069) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160035 YP_003035747.1 CDS ECBD_1500 NC_012947.1 1594143 1595192 R PFAM: conserved hypothetical protein; KEGG: sbo:SBO_2169 hypothetical protein; hypothetical protein complement(1594143..1595192) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160036 YP_003035748.1 CDS ECBD_1501 NC_012947.1 1595291 1596172 D PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: ssn:SSON_2213 DNA-binding transcriptional regulator; DNA-binding transcriptional regulator 1595291..1596172 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160037 YP_003035749.1 CDS ECBD_1502 NC_012947.1 1596377 1597846 D PFAM: amino acid permease-associated region; KEGG: sdy:SDY_2110 lysine transporter; lysine transporter 1596377..1597846 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160038 YP_003035750.1 CDS ECBD_1503 NC_012947.1 1598140 1600131 D Cir; FeuA; CirA; receptor protein for siderophores (colicin IA, IB and V) and microcins (E492, H47, and M); TonB-dependent; able to transport monomers, dimer, and linear trimers of 2,3-dihydorxybenzoylserine; outer membrane protein; colicin I receptor 1598140..1600131 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160039 YP_003035751.1 CDS ECBD_1504 NC_012947.1 1600163 1600999 R KEGG: sbc:SbBS512_E0812 S-formylglutathione hydrolase; TIGRFAM: S-formylglutathione hydrolase; PFAM: esterase; S-formylglutathione hydrolase complement(1600163..1600999) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160040 YP_003035752.1 CDS folE NC_012947.1 1601257 1601925 D involved in the first step of tetrahydrofolate biosynthesis; catalyzes the formation of formate and 2-amino-4-hydroxy-6-(erythro-1,2, 3-trihydroxypropyl)dihydropteridine triphosphate from GTP and water; forms a homopolymer; GTP cyclohydrolase I 1601257..1601925 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160041 YP_003035753.1 CDS ECBD_1506 NC_012947.1 1601942 1603099 D PFAM: protein of unknown function DUF418; protein of unknown function DUF405; KEGG: sbc:SbBS512_E0814 hypothetical protein; hypothetical protein 1601942..1603099 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160042 YP_003035754.1 CDS ECBD_1507 NC_012947.1 1603241 1604281 D negative regulator of the mglBAC operon for galactose utilization; DNA-binding transcriptional regulator GalS 1603241..1604281 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160043 YP_003035755.1 CDS ECBD_1509 NC_012947.1 1605615 1607135 D with MglBC transports galactose or methyl galactoside into the cell; contains 2 ATP binding domains; galactose/methyl galaxtoside transporter ATP-binding protein 1605615..1607135 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160045 YP_003035756.1 CDS mglC NC_012947.1 1607151 1608161 D ABC transporter; functions in galactose transport; part of MglA2C2B transporter complex; beta-methylgalactoside transporter inner membrane component 1607151..1608161 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160046 YP_003035757.1 CDS ECBD_1511 NC_012947.1 1608404 1609639 R catalyzes the first step in pyrimidine degradation: the NADPH-dependent reduction of uracil and thymine to the corresponding 5,6-dihydropyrimidines; dihydropyrimidine dehydrogenase complement(1608404..1609639) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160047 YP_003035758.1 CDS ECBD_1512 NC_012947.1 1609633 1610871 R function undetermined; similar to glutamate synthase beta subunit and related oxidoreductases which transfer electrons from NADPH to an acceptor protein or protein domain; oxidoreductase complement(1609633..1610871) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160048 YP_003035759.1 CDS ECBD_1513 NC_012947.1 1611065 1611304 R KEGG: sbc:SbBS512_E0826 hypothetical protein; hypothetical protein complement(1611065..1611304) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160049 YP_003035760.1 CDS ECBD_1514 NC_012947.1 1611307 1612026 R PFAM: protein of unknown function DUF218; KEGG: sfv:SFV_2219 hypothetical protein; hypothetical protein complement(1611307..1612026) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159319 YP_003035761.1 CDS ECBD_1515 NC_012947.1 1612176 1613060 R Reclaims exogenous and endogenous cytidine and 2'-deoxycytidine molecules for UMP synthesis; cytidine deaminase complement(1612176..1613060) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157505 YP_003035762.1 CDS ECBD_1516 NC_012947.1 1613190 1613885 R PFAM: LrgB family protein; KEGG: sbc:SbBS512_E0829 LrgB family protein; hypothetical protein complement(1613190..1613885) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157506 YP_003035763.1 CDS ECBD_1517 NC_012947.1 1613882 1614280 R PFAM: LrgA family protein; KEGG: sbo:SBO_1000 hypothetical protein; hypothetical protein complement(1613882..1614280) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157507 YP_003035764.1 CDS ECBD_1518 NC_012947.1 1614518 1615465 D PFAM: dihydrouridine synthase; KEGG: elf:LF82_0533 tRNA-dihydrouridine synthase C; tRNA-dihydrouridine synthase C 1614518..1615465 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157508 YP_003035765.1 CDS ECBD_1520 NC_012947.1 1616145 1617581 D may be involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride; multidrug resistance outer membrane protein MdtQ 1616145..1617581 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157510 YP_003035766.1 CDS ECBD_1521 NC_012947.1 1617634 1618395 D PFAM: short-chain dehydrogenase/reductase SDR; KEGG: sbc:SbBS512_E0834 3-oxoacyl-[acyl-carrier-protein] reductase; acetoin dehydrogenase 1617634..1618395 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157511 YP_003035767.1 CDS ECBD_1522 NC_012947.1 1618525 1619103 R PFAM: SNARE associated Golgi protein; KEGG: sbc:SbBS512_E0835 hypothetical protein; hypothetical protein complement(1618525..1619103) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157512 YP_003035768.1 CDS ECBD_1523 NC_012947.1 1619273 1619860 D PFAM: protein of unknown function DUF1282; KEGG: sbo:SBO_1009 hypothetical protein; hypothetical protein 1619273..1619860 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157513 YP_003035769.1 CDS pbpG NC_012947.1 1620025 1620966 D specifically hydrolyze the DD-diaminopimelate-alanine bonds in high-molecular-mass murein sacculi; Penicillin-binding protein 7; D-alanyl-D-alanine endopeptidase 1620025..1620966 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157514 YP_003035770.1 CDS ECBD_1525 NC_012947.1 1621004 1622719 R component of the membrane-bound D-lactate oxidase FAD-binding, NADH independent; D-lactate dehydrogenase complement(1621004..1622719) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157515 YP_003035771.1 CDS ECBD_1526 NC_012947.1 1622915 1625212 D PFAM: glycoside hydrolase; KEGG: ssn:SSON_2189 beta-D-glucoside glucohydrolase, periplasmic; glycoside hydrolase 1622915..1625212 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157516 YP_003035772.1 CDS ECBD_1527 NC_012947.1 1625464 1626381 D PFAM: substrate-binding region of ABC-type glycine betaine transporter; KEGG: sbo:SBO_1013 transporter permease; glycine/betaine ABC transporter substrate-binding protein 1625464..1626381 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157517 YP_003035773.1 CDS ECBD_1528 NC_012947.1 1626388 1627545 D PFAM: binding-protein-dependent transporters inner membrane component; KEGG: sbo:SBO_1014 transporter permease protein; binding-protein-dependent transporters inner membrane component 1626388..1627545 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157518 YP_003035774.1 CDS ECBD_1529 NC_012947.1 1627538 1628464 D PFAM: ABC transporter; SMART: ATPase AAA; KEGG: elf:LF82_3005 uncharacterized ABC transporter ATP-binding protein; ABC transporter 1627538..1628464 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157519 YP_003035775.1 CDS ECBD_1530 NC_012947.1 1628469 1629200 D PFAM: binding-protein-dependent transporters inner membrane component; KEGG: sbc:SbBS512_E0845 ABC transporter quaternary amine uptake (QAT) family, permease; binding-protein-dependent transporters inner membrane component 1628469..1629200 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157520 YP_003035776.1 CDS ECBD_1531 NC_012947.1 1629181 1629288 R KEGG: sdy:SDY_2161 hypothetical protein; hypothetical protein complement(1629181..1629288) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157521 YP_003035777.1 CDS ECBD_1532 NC_012947.1 1629348 1630079 R PFAM: regulatory protein MerR; SMART: regulatory protein MerR; KEGG: elf:LF82_1354 HTH-type transcriptional regulator MlrA; MerR family transcriptional regulator complement(1629348..1630079) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157522 YP_003035778.1 CDS ECBD_1533 NC_012947.1 1630301 1631986 D KEGG: sfx:S2325 2-component sensor protein; PFAM: histidine kinase internal region; GAF domain protein; 5TM Receptors of the LytS-YhcK type transmembrane region; ATP-binding region ATPase domain protein; SMART: GAF domain protein; signal transduction histidine kinase, LytS 1630301..1631986 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157523 YP_003035779.1 CDS ECBD_1534 NC_012947.1 1631983 1632702 D unknown function; when overproduced it confers drug-resistance; two-component response-regulatory protein YehT 1631983..1632702 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157524 YP_003035780.1 CDS ECBD_1535 NC_012947.1 1632749 1633219 D PFAM: protein of unknown function DUF1456; KEGG: ssn:SSON_2170 hypothetical protein; hypothetical protein 1632749..1633219 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157525 YP_003035781.1 CDS ECBD_1536 NC_012947.1 1633260 1633721 R PFAM: protein of unknown function DUF1307; KEGG: elf:LF82_3000 uncharacterized lipoprotein YehR; hypothetical protein complement(1633260..1633721) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157526 YP_003035782.1 CDS ECBD_1537 NC_012947.1 1633846 1635846 R PFAM: zinc finger SWIM domain protein; KEGG: ssn:SSON_2168 hypothetical protein; zinc finger SWIM domain protein complement(1633846..1635846) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157527 YP_003035783.1 CDS ECBD_1538 NC_012947.1 1635843 1636979 R PFAM: VWA containing CoxE family protein; SMART: von Willebrand factor A; KEGG: sbo:SBO_0944 hypothetical protein; VWA containing CoxE family protein complement(1635843..1636979) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157528 YP_003035784.1 CDS ECBD_1539 NC_012947.1 1636972 1639251 R KEGG: sfl:SF2189 hypothetical protein; hypothetical protein complement(1636972..1639251) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157529 YP_003035785.1 CDS ECBD_1540 NC_012947.1 1639262 1640350 R PFAM: ATPase associated with various cellular activities AAA_5; SMART: ATPase AAA; KEGG: sfx:S2313 hypothetical protein; ATPase associated with various cellular activities AAA_5 complement(1639262..1640350) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157530 YP_003035786.1 CDS ECBD_1541 NC_012947.1 1640657 1640974 R KEGG: efe:EFER_2203 hypothetical protein; hypothetical protein complement(1640657..1640974) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157531 YP_003035787.1 CDS ECBD_1542 NC_012947.1 1641035 1644667 R hypothetical protein complement(1641035..1644667) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157532 YP_003035788.1 CDS ECBD_1543 NC_012947.1 1644677 1648471 R PFAM: WGR domain protein; SMART: WGR domain protein; KEGG: efe:EFER_2201 molybdate metabolism regulator (MolR); WGR domain protein complement(1644677..1648471) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157533 YP_003035789.1 CDS metG NC_012947.1 1648612 1650645 R methionine--tRNA ligase; MetRS; adds methionine to tRNA(Met) with cleavage of ATP to AMP and diphosphate; some MetRS enzymes form dimers depending on a C-terminal domain that is also found in other proteins such as Trbp111 in Aquifex aeolicus and the cold-shock protein CsaA from Bacillus subtilis while others do not; four subfamilies exist based on sequence motifs and zinc content; methionyl-tRNA synthetase complement(1648612..1650645) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157534 YP_003035790.1 CDS ECBD_1545 NC_012947.1 1650777 1651886 D KEGG: sbc:SbBS512_E0855 mrp protein; ATPase 1650777..1651886 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157535 YP_003035791.1 CDS ECBD_1546 NC_012947.1 1652366 1652641 D PFAM: Insertion element protein; KEGG: dps:DP0861 insertion element iso-IS1d protein (InsA); Insertion element protein 1652366..1652641 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157536 YP_003035792.1 CDS ECBD_1547 NC_012947.1 1652560 1653063 D PFAM: IS1 transposase; KEGG: hdu:HD1970 insertion element IS1 1/5/6 protein InsB; IS1 transposase 1652560..1653063 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157537 YP_003035793.1 CDS ECBD_1548 NC_012947.1 1654512 1654703 D hypothetical protein 1654512..1654703 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157538 YP_003035794.1 CDS ECBD_1549 NC_012947.1 1654773 1654937 D hypothetical protein 1654773..1654937 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157539 YP_003035795.1 CDS ECBD_1550 NC_012947.1 1654897 1655562 D hypothetical protein 1654897..1655562 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157540 YP_003035796.1 CDS ECBD_1551 NC_012947.1 1655666 1656097 R KEGG: bvi:Bcep1808_7334 hypothetical protein; hypothetical protein complement(1655666..1656097) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157541 YP_003035797.1 CDS ECBD_1552 NC_012947.1 1656144 1656389 R KEGG: noc:Noc_3027 hypothetical protein; hypothetical protein complement(1656144..1656389) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157542 YP_003035798.1 CDS ECBD_1553 NC_012947.1 1656712 1657500 D catalyzes the formation of 4-methyl-5-(2-phosphoethyl)-thiazole and ADP from 4-methyl-5-(2-hydroxyethyl)-thiazole and ATP; hydroxyethylthiazole kinase 1656712..1657500 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157543 YP_003035799.1 CDS ECBD_1554 NC_012947.1 1657497 1658297 D catalyzes the formation of 4-amino-2-methyl-5-diphosphomethylpyrimidine; phosphomethylpyrimidine kinase 1657497..1658297 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157544 YP_003035800.1 CDS ECBD_1555 NC_012947.1 1658362 1659180 D PFAM: glycoside hydrolase family 25; SMART: glycoside hydrolase family 25; KEGG: ssn:SSON_2150 hypothetical protein; glycoside hydrolase family 25 1658362..1659180 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157545 YP_003035801.1 CDS ECBD_1556 NC_012947.1 1659232 1659978 D PFAM: UbiC transcription regulator-associated domain protein; regulatory protein GntR HTH; SMART: regulatory protein GntR HTH; KEGG: sdy:SDY_2274 transcriptional regulator; GntR family transcriptional regulator 1659232..1659978 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157546 YP_003035802.1 CDS ECBD_1557 NC_012947.1 1659952 1660917 R PFAM: PfkB domain protein; KEGG: sbo:SBO_0921 kinase; PfkB domain protein complement(1659952..1660917) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157547 YP_003035803.1 CDS ECBD_1558 NC_012947.1 1660914 1661918 R PFAM: ADP-ribosylation/crystallin J1; KEGG: ssn:SSON_2147 hypothetical protein; ADP-ribosylation/crystallin J1 complement(1660914..1661918) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157548 YP_003035804.1 CDS ECBD_1559 NC_012947.1 1661915 1663192 R TIGRFAM: nucleoside transporter; PFAM: nucleoside:H symporter; major facilitator superfamily MFS_1; KEGG: sbc:SbBS512_E1140 nucleoside transporter; nucleoside transporter complement(1661915..1663192) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157549 YP_003035805.1 CDS ECBD_1560 NC_012947.1 1663449 1664501 D catalyzes the formation of glycerone phosphate and D-glyceraldehyde 3-phosphate from D-fructose 1,6-bisphosphate; fructose-bisphosphate aldolase 1663449..1664501 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157550 YP_003035806.1 CDS gatY NC_012947.1 1664795 1665655 D catalyzes the reversible reaction of dihydroxyacetone phosphate with glyceraldehyde 3-phosphate to produce tagatose 1,6-bisphosphate; in enteric bacteria there are two D-tagatose 1,6-bisphosphate-specific aldolases: KbaY (also called AgaY), involved in catabolism of N-acetyl-galactosamine and D-galactosamine, and GatY which is part of the galactitol catabolism pathway; tagatose-bisphosphate aldolase 1664795..1665655 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157551 YP_003035807.1 CDS ECBD_1562 NC_012947.1 1665684 1666946 D TIGRFAM: D-tagatose-bisphosphate aldolase, class II, non-catalytic subunit; PFAM: D-tagatose-bisphosphate aldolase class II accessory protein AgaZ; KEGG: sbc:SbBS512_E1143 tagatose-6-phosphate kinase; D-tagatose-bisphosphate aldolase, class II, non-catalytic subunit 1665684..1666946 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157552 YP_003035808.1 CDS ECBD_1563 NC_012947.1 1666956 1667408 D phosphoenolpyruvate-dependent sugar phosphotransferase system; catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane; IIB is phosphorylated by IIA and then transfers the phosphoryl group to the sugar; IIC forms the translocation channel; PTS system galactitol-specific transporter subunit IIA 1666956..1667408 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157553 YP_003035809.1 CDS ECBD_1564 NC_012947.1 1667439 1667723 D phosphoenolpyruvate-dependent sugar phosphotransferase system; catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane; IIB is phosphorylated by IIA and then transfers the phosphoryl group to the sugar; IIC forms the translocation channel; PTS system galactitol-specific transporter subunit IIB 1667439..1667723 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157554 YP_003035810.1 CDS ECBD_1565 NC_012947.1 1667727 1669082 D TIGRFAM: PTS system, galactitol-specific IIC subunit; PFAM: PTS system Galactitol-specific IIC component; PTS system, galactitol-specific IIC subunit 1667727..1669082 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157555 YP_003035811.1 CDS ECBD_1566 NC_012947.1 1669130 1670170 D Zn-dependent; NAD(P)-binding; catalyzes the formation of tagatose-6-phosphate from galactitol-1-phosphate; galactitol-1-phosphate dehydrogenase 1669130..1670170 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157556 YP_003035812.1 CDS ECBD_1568 NC_012947.1 1670524 1671027 R PFAM: IS1 transposase; KEGG: dps:DP0860 insertion element IS1 1/5/6 protein (InsB); IS1 transposase complement(1670524..1671027) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157558 YP_003035813.1 CDS ECBD_1569 NC_012947.1 1670946 1671221 R PFAM: Insertion element protein; KEGG: hdu:HD1971 insertion element IS1 1/2/3/5/6 protein InsA; Insertion element protein complement(1670946..1671221) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157559 YP_003035814.1 CDS ECBD_1570 NC_012947.1 1671907 1672806 R cytosolic protein with phosphatidylglycerol kinase activity; undetermined physiological role; lipid kinase complement(1671907..1672806) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157560 YP_003035815.1 CDS ECBD_1571 NC_012947.1 1673211 1673528 D KEGG: sbo:SBO_0909 hypothetical protein; hypothetical protein 1673211..1673528 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157561 YP_003035816.1 CDS ECBD_1572 NC_012947.1 1673859 1675220 R PFAM: peptidase U32; KEGG: ssn:SSON_2134 hypothetical protein; peptidase U32 complement(1673859..1675220) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157562 YP_003035817.1 CDS ECBD_1573 NC_012947.1 1675366 1675698 R PFAM: protein of unknown function DUF1508; KEGG: ssn:SSON_2133 hypothetical protein; hypothetical protein complement(1675366..1675698) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157563 YP_003035818.1 CDS ECBD_1574 NC_012947.1 1675889 1676611 R response regulator in two-component regulatory system with BaeS; regulator of RNA synthesis, flagellar biosynthesis, chemotaxis and transport; DNA-binding transcriptional regulator BaeR complement(1675889..1676611) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157564 YP_003035819.1 CDS ECBD_1575 NC_012947.1 1676608 1678011 R PFAM: ATP-binding region ATPase domain protein; histidine kinase A domain protein; histidine kinase HAMP region domain protein; SMART: ATP-binding region ATPase domain protein; histidine kinase A domain protein; histidine kinase HAMP region domain protein; KEGG: sbc:SbBS512_E1155 sensor histidine kinase BaeS; signal transduction histidine-protein kinase BaeS complement(1676608..1678011) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157565 YP_003035820.1 CDS ECBD_1576 NC_012947.1 1678008 1679423 R PFAM: major facilitator superfamily MFS_1; KEGG: sbc:SbBS512_E1156 multidrug resistance protein MdtD; multidrug efflux system protein MdtE complement(1678008..1679423) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157566 YP_003035821.1 CDS ECBD_1577 NC_012947.1 1679424 1682501 R Part of a tripartite efflux system composed of MdtA, MdtB and MdtC; confers resistance against novobiocin and deoxycholate; multidrug efflux system subunit MdtC complement(1679424..1682501) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157567 YP_003035822.1 CDS ECBD_1578 NC_012947.1 1682502 1685624 R Part of a tripartite efflux system composed of MdtA, MdtB and MdtC which confers resistance against novobiocin and deoxycholate; multidrug efflux system subunit MdtB complement(1682502..1685624) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157568 YP_003035823.1 CDS ECBD_1579 NC_012947.1 1685624 1686871 R Part of a tripartite efflux system composed of MdtA, MdtB and MdtC which confers resistance against novobiocin and deoxycholate; multidrug efflux system subunit MdtA complement(1685624..1686871) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157569 YP_003035824.1 CDS ECBD_1580 NC_012947.1 1687041 1687169 R hypothetical protein complement(1687041..1687169) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157570 YP_003035825.1 CDS ECBD_1581 NC_012947.1 1687369 1687515 R hypothetical protein complement(1687369..1687515) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157571 YP_003035826.1 CDS ECBD_1582 NC_012947.1 1687697 1687843 R hypothetical protein complement(1687697..1687843) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157572 YP_003035827.1 CDS ECBD_1583 NC_012947.1 1688416 1689075 D PFAM: von Willebrand factor A; SMART: von Willebrand factor A; KEGG: sbo:SBO_0899 hypothetical protein; von Willebrand factor A 1688416..1689075 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157573 YP_003035828.1 CDS ECBD_1584 NC_012947.1 1689072 1689833 D KEGG: sbc:SbBS512_E1161 hypothetical protein; hypothetical protein 1689072..1689833 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157574 YP_003035829.1 CDS ECBD_1585 NC_012947.1 1689830 1691770 D KEGG: sbc:SbBS512_E1162 hypothetical protein; hypothetical protein 1689830..1691770 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157575 YP_003035830.1 CDS ECBD_1586 NC_012947.1 1691783 1693135 R KEGG: elf:LF82_2973 uncharacterized chaperone protein YegD; chaperone complement(1691783..1693135) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157576 YP_003035831.1 CDS ECBD_1587 NC_012947.1 1693269 1694117 D inducible, catalyzes the hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine; 3-methyl-adenine DNA glycosylase II 1693269..1694117 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157577 YP_003035832.1 CDS ECBD_1588 NC_012947.1 1694218 1697535 R contains a MASE1 (membrane-Associated Sensor) domain; potentially involved in signal transduction; sensor protein complement(1694218..1697535) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157578 YP_003035833.1 CDS ECBD_1589 NC_012947.1 1697853 1698494 D functions in pyrimidine salvage; pyrimidine ribonucleoside kinase; phosphorylates nucleosides or dinucleosides to make UMP or CMP using ATP or GTP as the donor; uridine kinase 1697853..1698494 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157579 YP_003035834.1 CDS dcd NC_012947.1 1698586 1699167 D Catalyzes the formation of dUTP from dCTP in thymidylate biosynthesis; deoxycytidine triphosphate deaminase 1698586..1699167 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157580 YP_003035835.1 CDS ECBD_1591 NC_012947.1 1699189 1701042 D YegA; inner membrane protein involved in the assembly of outer membrane proteins (OMPs); asmA-null mutants show low lipopolysaccharide (LPS) levels, suggesting a role in LPS biogenesis and/or in restricting outer membrane fluidity, resulting on altered assembly of OMPs; assembly protein 1699189..1701042 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157581 YP_003035836.1 CDS ECBD_1592 NC_012947.1 1701316 1702899 R PFAM: integral membrane protein TerC; CBS domain containing protein; transporter-associated region; KEGG: sbo:SBO_0890 transporter; integral membrane protein TerC complement(1701316..1702899) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157582 YP_003035837.1 CDS ECBD_1593 NC_012947.1 1703558 1704697 D PFAM: polysaccharide export protein; KEGG: ssn:SSON_2115 polysaccharide export protein; polysaccharide export protein 1703558..1704697 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157583 YP_003035838.1 CDS ECBD_1594 NC_012947.1 1704703 1705146 D Wzb shows phosphatase activity towards the autophosphorylated Wzc protein, which induces colanic acid biosynthesis; catalyzes the phosphorylation of UDP-glucose dehydrogenase, an enzyme involved in colanic acid biosynthesis; tyrosine phosphatase 1704703..1705146 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157584 YP_003035839.1 CDS ECBD_1595 NC_012947.1 1705149 1707311 D Wzc; catalyzes the autophosphorylation on tyrosine residues which downregulates the biosynthesis of colonic acid (an extracellular polysaccharide); tyrosine kinase 1705149..1707311 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157585 YP_003035840.1 CDS ECBD_1596 NC_012947.1 1707404 1708243 D PFAM: glycosyl transferase family 2; KEGG: sfv:SFV_2118 glycosyl transferase; glycosyl transferase 1707404..1708243 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157424 YP_003035841.1 CDS ECBD_1597 NC_012947.1 1708246 1708734 D acetyltransferase believed to catalyze the addition of the acetyl group that is attached through an O linkage to the first fucosyl residue of the colanic acid repetitive unit (E unit); wcaB is induced in sessile bacteria and by osmotic shock, and repressed when grown in rich medium; colanic acid biosynthesis acetyltransferase WcaB 1708246..1708734 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156418 YP_003035842.1 CDS ECBD_1598 NC_012947.1 1708731 1709948 D KEGG: ssn:SSON_2110 predicted glycosyl transferase; glycosyl transferase 1708731..1709948 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156419 YP_003035843.1 CDS wcaD NC_012947.1 1709923 1711140 D essential for colanic acid biosynthesis; colanic acid is an exopolysaccharide produced under stress conditions that confers acid and heat tolerance; colanic acid biosynthesis protein 1709923..1711140 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156420 YP_003035844.1 CDS ECBD_1600 NC_012947.1 1711151 1711897 D PFAM: glycosyl transferase family 2; KEGG: ssn:SSON_2108 predicted glycosyl transferase; glycosyl transferase 1711151..1711897 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156421 YP_003035845.1 CDS ECBD_1601 NC_012947.1 1711913 1712461 D acetyltransferase believed to catalyze the addition of the acetyl group that is attached through an O linkage to the first fucosyl residue of the colanic acid repetitive unit (E unit); colanic acid biosynthesis acetyltransferase WcaF 1711913..1712461 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156422 YP_003035846.1 CDS ECBD_1602 NC_012947.1 1712488 1713609 D TIGRFAM: GDP-mannose 4,6-dehydratase; PFAM: NAD-dependent epimerase/dehydratase; KEGG: ssn:SSON_2106 GDP-D-mannose dehydratase; GDP-mannose 4,6-dehydratase 1712488..1713609 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156423 YP_003035847.1 CDS ECBD_1603 NC_012947.1 1713612 1714577 D PFAM: NAD-dependent epimerase/dehydratase; KEGG: ssn:SSON_2105 nucleotide di-P-sugar epimerase or dehydratase; NAD-dependent epimerase/dehydratase 1713612..1714577 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156424 YP_003035848.1 CDS ECBD_1604 NC_012947.1 1714577 1715059 D PFAM: NUDIX hydrolase; KEGG: ssn:SSON_2104 GDP-mannose mannosyl hydrolase; NUDIX hydrolase 1714577..1715059 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156425 YP_003035849.1 CDS ECBD_1605 NC_012947.1 1715056 1716279 D PFAM: glycosyl transferase group 1; KEGG: sfx:S2236 glycosyl transferase; glycosyl transferase 1715056..1716279 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156426 YP_003035850.1 CDS ECBD_1606 NC_012947.1 1716282 1717718 D KEGG: elf:LF82_0345 mannose-1-phosphate guanylyltransferase [GDP]; TIGRFAM: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase; PFAM: nucleotidyl transferase; cupin; mannose-6-phosphate isomerase type II; mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase 1716282..1717718 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156427 YP_003035851.1 CDS ECBD_1607 NC_012947.1 1717911 1719281 D PFAM: phosphoglucomutase/phosphomannomutase alpha/beta/alpha domain II; phosphoglucomutase/phosphomannomutase alpha/beta/alpha domain III; phosphoglucomutase/phosphomannomutase alpha/beta/alpha domain I; phosphoglucomutase/phosphomannomutase; KEGG: efe:EFER_2132 phosphomannomutase; phosphomannomutase 1717911..1719281 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156428 YP_003035852.1 CDS ECBD_1608 NC_012947.1 1719336 1720730 D WcaJ; glucose-1-phosphate transferase responsible for the addition of the first glucose-1-phosphate from UDP-glucose to the lipid carrier undecaprenyl phosphate in the biosynthesis of colanic acid; UDP-glucose lipid carrier transferase 1719336..1720730 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156429 YP_003035853.1 CDS ECBD_1609 NC_012947.1 1720732 1722210 D PFAM: polysaccharide biosynthesis protein; colanic acid exporter 1720732..1722210 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156430 YP_003035854.1 CDS ECBD_1610 NC_012947.1 1722372 1723652 D PFAM: polysaccharide pyruvyl transferase; KEGG: sbo:SBO_0872 pyruvyl transferase; pyruvyl transferase 1722372..1723652 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156431 YP_003035855.1 CDS ECBD_1611 NC_012947.1 1723649 1724869 D PFAM: glycosyl transferase group 1; KEGG: efe:EFER_2128 glycosyl transferase; glycosyl transferase group 1 1723649..1724869 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156432 YP_003035856.1 CDS wcaM NC_012947.1 1724880 1726274 D WcaM from Salmonella enterica is essential for biofilm formation on HEp-2 cells and chicken intestinal tissue but not on a plastic surface; may be involved in the biosynthesis of colanic acid; colanic acid biosynthesis protein 1724880..1726274 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156433 YP_003035857.1 CDS ECBD_1613 NC_012947.1 1726449 1727342 D together with GalU subunit composes the UTP--glucose-1-phosphate uridylyltransferase, an enzyme that catalyzes the formation of UDP-glucose from UTP and alpha-D-glucose 1-phosphate; regulates cellular levels of UDP-glucose; UTP--glucose-1-phosphate uridylyltransferase subunit GalF 1726449..1727342 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156434 YP_003035858.1 CDS ECBD_1614 NC_012947.1 1727715 1728800 D NAD(P) binding; catalyzes the formation of dTDP-4-dehydro-6-deoxy-D-glucose from dTDP-glucose; dTDP-glucose 4,6 dehydratase 1727715..1728800 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156435 YP_003035859.1 CDS ECBD_1615 NC_012947.1 1728800 1729699 D with dTDP-4-dehydrorhamnose 3,5-epimerase forms a complex known as dTDP-L-rhamnose synthetase; catalyzes the reduction of dTDP-4-dehydro-6-deoxy-L-mannose to dTDP-L-rhamnose; dTDP-4-dehydrorhamnose reductase 1728800..1729699 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156436 YP_003035860.1 CDS ECBD_1616 NC_012947.1 1729758 1730636 D TIGRFAM: glucose-1-phosphate thymidylyltransferase; PFAM: nucleotidyl transferase; KEGG: sfv:SFV_2096 glucose-1-phosphate thymidylyltransferase; glucose-1-phosphate thymidylyltransferase 1729758..1730636 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156437 YP_003035861.1 CDS ECBD_1617 NC_012947.1 1730641 1731186 D KEGG: sfv:SFV_2095 dTDP-6-deoxy-L-mannose-dehydrogenase; TIGRFAM: dTDP-4-dehydrorhamnose 3,5-epimerase; PFAM: dTDP-4-dehydrorhamnose 35-epimerase related; dTDP-4-dehydrorhamnose 3,5-epimerase 1730641..1731186 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156438 YP_003035862.1 CDS ECBD_1618 NC_012947.1 1731190 1732614 D PFAM: polysaccharide biosynthesis protein; KEGG: shw:Sputw3181_1467 polysaccharide biosynthesis protein; polysaccharide biosynthesis protein 1731190..1732614 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156439 YP_003035863.1 CDS ECBD_1619 NC_012947.1 1732624 1733736 D PFAM: DegT/DnrJ/EryC1/StrS aminotransferase; KEGG: she:Shewmr4_1321 DegT/DnrJ/EryC1/StrS aminotransferase; DegT/DnrJ/EryC1/StrS aminotransferase 1732624..1733736 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156440 YP_003035864.1 CDS ECBD_1620 NC_012947.1 1733738 1734316 D KEGG: she:Shewmr4_1322 acetyltransferase; acetyltransferase 1733738..1734316 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156441 YP_003035865.1 CDS ECBD_1621 NC_012947.1 1734313 1735059 D KEGG: pub:SAR11_0820 rhamnosyltransferase; hypothetical protein 1734313..1735059 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156442 YP_003035866.1 CDS ECBD_1622 NC_012947.1 1735059 1735880 D hypothetical protein 1735059..1735880 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156443 YP_003035867.1 CDS ECBD_1623 NC_012947.1 1735877 1737052 D KEGG: son:SO_3179 lipopolysaccharide biosynthesis polymerase; hypothetical protein 1735877..1737052 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156444 YP_003035868.1 CDS ECBD_1624 NC_012947.1 1737049 1738161 D PFAM: glycosyl transferase group 1; KEGG: aci:ACIAD0084 mannosyl transferase; glycosyl transferase group 1 1737049..1738161 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156445 YP_003035869.1 CDS ECBD_1626 NC_012947.1 1738969 1739244 D PFAM: Insertion element protein; KEGG: hdu:HD1971 insertion element IS1 1/2/3/5/6 protein InsA; Insertion element protein 1738969..1739244 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156447 YP_003035870.1 CDS ECBD_1627 NC_012947.1 1739163 1739666 D PFAM: IS1 transposase; KEGG: sty:HCM1.136a IS1 transposase; IS1 transposase 1739163..1739666 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156448 YP_003035871.1 CDS ECBD_1628 NC_012947.1 1739752 1741176 D KEGG: efe:EFER_2133 mannose-1-phosphate guanyltransferase; TIGRFAM: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase; PFAM: nucleotidyl transferase; cupin; mannose-6-phosphate isomerase type II; mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase 1739752..1741176 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156449 YP_003035872.1 CDS ECBD_1629 NC_012947.1 1741210 1742574 D PFAM: phosphoglucomutase/phosphomannomutase alpha/beta/alpha domain II; phosphoglucomutase/phosphomannomutase alpha/beta/alpha domain III; phosphoglucomutase/phosphomannomutase alpha/beta/alpha domain I; phosphoglucomutase/phosphomannomutase; KEGG: ssn:SSON_2101 phosphomannomutase; phosphomannomutase 1741210..1742574 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156450 YP_003035873.1 CDS ECBD_1630 NC_012947.1 1742738 1744144 D catalyzes the formation of D-ribulose 5-phosphate from 6-phospho-D-gluconate; 6-phosphogluconate dehydrogenase 1742738..1744144 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156451 YP_003035874.1 CDS ECBD_1631 NC_012947.1 1744392 1745558 D TIGRFAM: nucleotide sugar dehydrogenase; PFAM: UDP-glucose/GDP-mannose dehydrogenase; UDP-glucose/GDP-mannose dehydrogenase dimerisation; UDP-glucose/GDP-mannose dehydrogenase; nucleotide sugar dehydrogenase 1744392..1745558 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156452 YP_003035875.1 CDS ECBD_1632 NC_012947.1 1745704 1746684 D PFAM: lipopolysaccharide biosynthesis protein; KEGG: ssn:SSON_2098 regulator of length of O-antigen component of lipopolysaccharide chains; lipopolysaccharide biosynthesis protein 1745704..1746684 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156453 YP_003035876.1 CDS ECBD_1633 NC_012947.1 1746780 1747391 R catalyzes the formation of 1-(5-phosphoribosyl)-AMP from 1-(5-phosphoribosyl)-ATP and the subsequent formation of 1-(5-phosphoribosyl)-5-((5- phosphoribosylamino)methylideneamino)imidazole-4- carboxamide from 1-(5-phosphoribosyl)-AMP in histidine biosynthesis; bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein complement(1746780..1747391) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156454 YP_003035877.1 CDS ECBD_1634 NC_012947.1 1747385 1748161 R catalyzes the conversion of 5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]- 1-(5-phosphoribosyl)imidazole-4-carboxamideand glutamine to imidazole-glycerol phosphate, 5-aminoimidazol-4-carboxamideribonucleotide and glutamate; the HisF subunit acts as a cyclase; imidazole glycerol phosphate synthase subunit HisF complement(1747385..1748161) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156455 YP_003035878.1 CDS ECBD_1635 NC_012947.1 1748143 1748880 R catalyzes the formation of 5-(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino-l- (5-phosphoribosyl)imidazole-4-carboxamide from 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide; 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase complement(1748143..1748880) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156456 YP_003035879.1 CDS hisH NC_012947.1 1748880 1749470 R with HisF IGPS catalyzes the conversion of phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide ribonucleotide phosphate and glutamine to imidazole-glycerol phosphate, 5-aminoimidazol-4-carboxamide ribonucleotide, and glutamate in histidine biosynthesis; the HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of imidazole-glycerol phosphate and 5-aminoimidazol-4-carboxamide ribonucleotide; imidazole glycerol phosphate synthase subunit HisH complement(1748880..1749470) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156457 YP_003035880.1 CDS ECBD_1637 NC_012947.1 1749470 1750537 R catalyzes the formation of 3-(imidazol-4-yl)-2-oxopropyl phosphate from D-ethythro-1-(imidazol-4-yl)glycerol 3-phosphate and histidinol from histidinol phosphate; imidazole glycerol-phosphate dehydratase/histidinol phosphatase complement(1749470..1750537) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156458 YP_003035881.1 CDS ECBD_1638 NC_012947.1 1750537 1751607 R catalyzes the formation of L-histidinol phosphate from imidazole-acetol phosphate and glutamate in histidine biosynthesis; histidinol-phosphate aminotransferase complement(1750537..1751607) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156459 YP_003035882.1 CDS hisD NC_012947.1 1751604 1752908 R catalyzes the oxidation of L-histidinol to L-histidinaldehyde and then to L-histidine in histidine biosynthesis; functions as a dimer; histidinol dehydrogenase complement(1751604..1752908) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156460 YP_003035883.1 CDS hisG NC_012947.1 1752914 1753813 R long form of enzyme; catalyzes the formation of N'-5'-phosphoribosyl-ATP from phosphoribosyl pyrophosphate; crucial role in histidine biosynthesis; forms active dimers and inactive hexamers which is dependent on concentration of substrates and inhibitors; ATP phosphoribosyltransferase complement(1752914..1753813) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156461 YP_003035884.1 CDS ECBD_1641 NC_012947.1 1754436 1755260 D PFAM: NAD-dependent epimerase/dehydratase; KEGG: ssn:SSON_2086 enzyme of sugar metabolism; NAD-dependent epimerase/dehydratase 1754436..1755260 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156462 YP_003035885.1 CDS ECBD_1642 NC_012947.1 1755285 1756235 D PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: ssn:SSON_2085 transcriptional regulator LysR-type; LysR family transcriptional regulator 1755285..1756235 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156463 YP_003035886.1 CDS ECBD_1643 NC_012947.1 1756502 1757860 D PFAM: amino acid permease-associated region; KEGG: sdy:SDY_2226 amino acid/amine transporter; amino acid permease-associated region 1756502..1757860 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156464 YP_003035887.1 CDS ECBD_1644 NC_012947.1 1758039 1759097 D PFAM: protein of unknown function DUF395 YeeE/YedE; KEGG: efe:EFER_2096 conserved hypothetical protein; inner membrane protein; hypothetical protein 1758039..1759097 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156465 YP_003035888.1 CDS ECBD_1645 NC_012947.1 1759111 1759338 D PFAM: SirA family protein; KEGG: ssn:SSON_2082 hypothetical protein; SirA family protein 1759111..1759338 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156466 YP_003035889.1 CDS sbcB NC_012947.1 1759381 1760808 R Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates; exonuclease I complement(1759381..1760808) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156467 YP_003035890.1 CDS dacD NC_012947.1 1761017 1762183 D removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors; penicillin-binding protein 6B; one of four, DD-carboxypeptidase low-molecular weight penicillin-binding proteins that remove terminal D-alanine from pentapeptide side chains; D-alanyl-D-alanine carboxypeptidase 1761017..1762183 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156468 YP_003035891.1 CDS ECBD_1648 NC_012947.1 1762302 1762775 D PFAM: transcription activator effector binding; DNA gyrase inhibitor 1762302..1762775 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156469 YP_003035892.1 CDS ECBD_1649 NC_012947.1 1762973 1764031 D membrane protein-like protein 1762973..1764031 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156470 YP_003035893.1 CDS ECBD_1650 NC_012947.1 1764203 1764532 D PFAM: protein of unknown function DUF496; KEGG: ssn:SSON_2077 hypothetical protein; hypothetical protein 1764203..1764532 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156471 YP_003035894.1 CDS ECBD_1651 NC_012947.1 1765680 1766081 D hypothetical protein 1765680..1766081 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156472 YP_003035895.1 CDS ECBD_1654 NC_012947.1 1766832 1767224 R KEGG: cko:CKO_02654 hypothetical protein; hypothetical protein complement(1766832..1767224) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156475 YP_003035896.1 CDS cobU NC_012947.1 1768896 1769441 D catalyzes ATP-dependent phosphorylation of adenosylcobinamide to form adenosylcobinamide phosphate and the addition of guanosine monophosphate to adenosylcobinamide phosphate to form adenosylcobinamide-GDP; adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase 1768896..1769441 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156477 YP_003035897.1 CDS cobS NC_012947.1 1769438 1770181 D catalyzes the formation of adenosylcobalamin from Ado-cobinamide-GDP and alpha-ribazole; cobalamin synthase 1769438..1770181 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156478 YP_003035898.1 CDS cobT NC_012947.1 1770193 1771272 D catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide and 5,6-dimethylbenzimidazole; nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase 1770193..1771272 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156479 YP_003035899.1 CDS ECBD_1659 NC_012947.1 1771334 1772269 D PFAM: ErfK/YbiS/YcfS/YnhG family protein; KEGG: sdy:SDY_2243 hypothetical protein; hypothetical protein 1771334..1772269 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156480 YP_003035900.1 CDS ECBD_1660 NC_012947.1 1772727 1773644 D transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation; nitrogen assimilation transcriptional regulator 1772727..1773644 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156482 YP_003035901.1 CDS cbl NC_012947.1 1773746 1774696 D LysR-type transcriptional regulator similar to CysB; contains helix-turn-helix (HTH) motif; in Escherichia coli this protein regulates two operons consisting of ABC transporters that are part of the cys regulon; regulated by CysB; transcriptional regulator Cbl 1773746..1774696 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156483 YP_003035902.1 CDS ECBD_1662 NC_012947.1 1774970 1776457 D TIGRFAM: MATE efflux family protein; PFAM: multi antimicrobial extrusion protein MatE; KEGG: ssn:SSON_2044 predicted multidrug efflux system; hypothetical protein 1774970..1776457 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156485 YP_003035903.1 CDS ECBD_1663 NC_012947.1 1777087 1777803 R PFAM: protein of unknown function DUF28; KEGG: sbo:SBO_1217 hypothetical protein; hypothetical protein complement(1777087..1777803) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156487 YP_003035904.1 CDS ECBD_1664 NC_012947.1 1778146 1779600 R Catalyzes the hydrolysis of AMP to form adenine and ribose 5-phosphate using water as the nucleophile; AMP nucleosidase complement(1778146..1779600) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156488 YP_003035905.1 CDS ECBD_1665 NC_012947.1 1779702 1781018 R TIGRFAM: metabolite/H+ symporter, major facilitator superfamily (MFS); PFAM: major facilitator superfamily MFS_1; general substrate transporter; shikimate transporter complement(1779702..1781018) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156489 YP_003035906.1 CDS ECBD_1667 NC_012947.1 1782647 1789723 R PFAM: Ig domain protein group 1 domain protein; SMART: Ig domain protein group 1 domain protein; PKD domain containing protein; Ig domain protein group 1 domain protein complement(1782647..1789723) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156491 YP_003035907.1 CDS ECBD_1668 NC_012947.1 1789764 1789913 R hypothetical protein complement(1789764..1789913) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156492 YP_003035908.1 CDS ECBD_1669 NC_012947.1 1790213 1791010 R PFAM: protein of unknown function DUF980; KEGG: ssn:SSON_2035 hypothetical protein; hypothetical protein complement(1790213..1791010) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156494 YP_003035909.1 CDS ECBD_1670 NC_012947.1 1791810 1793018 R PFAM: transposase IS4 family protein; KEGG: apa:APP7_1819 iso-IS10R ORF; transposase IS4 family protein complement(1791810..1793018) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156496 YP_003035910.1 CDS ECBD_1672 NC_012947.1 1793769 1794419 D PFAM: YodA domain protein; KEGG: sbc:SbBS512_E0910 metal-binding protein; hypothetical protein 1793769..1794419 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156498 YP_003035911.1 CDS ECBD_1673 NC_012947.1 1794762 1795271 D PFAM: cytochrome B561; KEGG: sbo:SBO_1032 cytochrome; cytochrome B561 1794762..1795271 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158073 YP_003035912.1 CDS ECBD_1674 NC_012947.1 1795281 1796489 R PFAM: transposase IS4 family protein; KEGG: sea:SeAg_B3181 IS10 transposase; transposase IS4 family protein complement(1795281..1796489) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157664 YP_003035913.1 CDS ECBD_1675 NC_012947.1 1796549 1796854 R KEGG: sdy:SDY_1034 sulfite oxidase subunit YedZ; sulfite oxidase subunit YedZ complement(1796549..1796854) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157665 YP_003035914.1 CDS ECBD_1676 NC_012947.1 1796855 1797859 R in Escherichia coli this periplasmic enzyme was found to encode the periplasmic catalytic subunit of an oxidoreductase; sulfite oxidase activity not demonstrated; requires inner membrane anchor protein YedZ; sulfite oxidase subunit YedY complement(1796855..1797859) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157666 YP_003035915.1 CDS ECBD_1677 NC_012947.1 1797968 1798381 R KEGG: sfv:SFV_2015 hypothetical protein; TIGRFAM: hydroxyisourate hydrolase; PFAM: transthyretin; SMART: transthyretin; hydroxyisourate hydrolase complement(1797968..1798381) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157667 YP_003035916.1 CDS ECBD_1678 NC_012947.1 1798403 1799185 D induced by CusR in the presence of copper; YedW induces the expression of the upstream gene yedV (encoding a sensor kinase) as well as yedW; yedVW is one of four copper regulons found in E. coli; part of the copper homeostasis mechanism; confers resistance to copper and several drugs when induced; transcriptional regulatory protein YedW 1798403..1799185 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157668 YP_003035917.1 CDS ECBD_1679 NC_012947.1 1799185 1800543 D KEGG: ssn:SSON_2026 2-component sensor protein; TIGRFAM: heavy metal sensor kinase; PFAM: ATP-binding region ATPase domain protein; histidine kinase HAMP region domain protein; histidine kinase A domain protein; SMART: ATP-binding region ATPase domain protein; histidine kinase A domain protein; heavy metal sensor signal transduction histidine kinase 1799185..1800543 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157669 YP_003035918.1 CDS ECBD_1680 NC_012947.1 1800651 1801502 R Hsp31 stabilizes early unfolding protein intermediates under severe heat stress; chaperone protein HchA complement(1800651..1801502) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157670 YP_003035919.1 CDS ECBD_1681 NC_012947.1 1801523 1801666 R hypothetical protein complement(1801523..1801666) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157671 YP_003035920.1 CDS ECBD_1683 NC_012947.1 1803916 1804218 D KEGG: sbo:SBO_1045 hypothetical protein; hypothetical protein 1803916..1804218 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157673 YP_003035921.1 CDS ECBD_1684 NC_012947.1 1804258 1804953 D PFAM: metal-dependent phosphohydrolase HD sub domain; SMART: metal-dependent phosphohydrolase HD region; KEGG: sbc:SbBS512_E0922 metal-dependent phosphohydrolase; hypothetical protein 1804258..1804953 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157674 YP_003035922.1 CDS ECBD_1685 NC_012947.1 1805020 1806438 D TIGRFAM: DNA-cytosine methyltransferase; PFAM: C-5 cytosine-specific DNA methylase; DNA cytosine methylase 1805020..1806438 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157675 YP_003035923.1 CDS ECBD_1686 NC_012947.1 1806419 1806889 D TIGRFAM: DNA mismatch endonuclease Vsr; PFAM: DNA mismatch endonuclease vsr; KEGG: elf:LF82_2418 very short patch repair protein; DNA mismatch endonuclease Vsr 1806419..1806889 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157676 YP_003035924.1 CDS ECBD_1687 NC_012947.1 1806878 1807798 R TIGRFAM: carboxylate/amino acid/amine transporter; PFAM: protein of unknown function DUF6 transmembrane; KEGG: elf:LF82_2949 uncharacterized inner membrane transporter YedA; hypothetical protein complement(1806878..1807798) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157677 YP_003035925.1 CDS ECBD_1688 NC_012947.1 1807971 1808888 D PFAM: protein of unknown function DUF808; KEGG: sdy:SDY_1048 hypothetical protein; hypothetical protein 1807971..1808888 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157678 YP_003035926.1 CDS ECBD_1689 NC_012947.1 1808967 1809149 D KEGG: ssn:SSON_2014 hypothetical protein; hypothetical protein 1808967..1809149 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157679 YP_003035927.1 CDS ECBD_1690 NC_012947.1 1809320 1811014 D KEGG: ssn:SSON_2013 hypothetical protein; TIGRFAM: diguanylate cyclase; PFAM: GGDEF domain containing protein; SMART: GGDEF domain containing protein; diguanylate cyclase 1809320..1811014 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157680 YP_003035928.1 CDS ECBD_1691 NC_012947.1 1811011 1811826 R catalyzes the hydrolysis of mannosyl-3-phosphoglycerate to form the osmolyte mannosylglycerate; mannosyl-3-phosphoglycerate phosphatase complement(1811011..1811826) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157681 YP_003035929.1 CDS ECBD_1692 NC_012947.1 1812124 1812351 R KEGG: sdy:SDY_1052 hypothetical protein; hypothetical protein complement(1812124..1812351) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157682 YP_003035930.1 CDS ECBD_1693 NC_012947.1 1812514 1812702 D KEGG: ssn:SSON_2010 hypothetical protein; hypothetical protein 1812514..1812702 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157683 YP_003035931.1 CDS ECBD_1694 NC_012947.1 1812746 1813369 R PFAM: regulatory protein LuxR; SMART: regulatory protein LuxR; KEGG: sfv:SFV_1994 positive regulator for ctr capsule biosynthesis, positive transcription factor; LuxR family transcriptional regulator complement(1812746..1813369) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157684 YP_003035932.1 CDS fliR NC_012947.1 1813659 1814444 R FliR, with proteins FliP and FliQ, forms the core of the central channel in the flagella export apparatus; flagellar biosynthesis protein FliR complement(1813659..1814444) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157685 YP_003035933.1 CDS fliQ NC_012947.1 1814452 1814721 R with proteins FliP and FliR forms the core of the central channel in the flagella export apparatus; flagellar biosynthesis protein FliQ complement(1814452..1814721) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157686 YP_003035934.1 CDS fliP NC_012947.1 1814731 1815468 R FliP, with proteins FliQ and FliR, forms the core of the central channel in the flagella export apparatus; flagellar biosynthesis protein FliP complement(1814731..1815468) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157687 YP_003035935.1 CDS ECBD_1698 NC_012947.1 1815468 1815833 R with FlhA, FlhB, FliP, FliQ and FliR is one of the membrane components of the flagellar export apparatus; flagellar biosynthesis protein FliO complement(1815468..1815833) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157688 YP_003035936.1 CDS fliN NC_012947.1 1815836 1816249 R One of three proteins involved in switching the direction of the flagellar rotation; flagellar motor switch protein FliN complement(1815836..1816249) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157689 YP_003035937.1 CDS fliM NC_012947.1 1816246 1817250 R with FliG and FliN makes up the switch complex which is involved in switching the direction of the flagella rotation; flagellar motor switch protein FliM complement(1816246..1817250) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157690 YP_003035938.1 CDS fliL NC_012947.1 1817255 1817719 R interacts with cytoplasmic MS ring of the basal body and may act to stabilize the MotAB complexes which surround the MS ring; flagellar basal body-associated protein FliL complement(1817255..1817719) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157691 YP_003035939.1 CDS ECBD_1702 NC_012947.1 1817824 1818951 R PFAM: flagellar hook-length control protein; flagellar hook-length control protein complement(1817824..1818951) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157692 YP_003035940.1 CDS fliJ NC_012947.1 1818948 1819391 R rod/hook and filament chaperone; flagellar biosynthesis chaperone complement(1818948..1819391) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157693 YP_003035941.1 CDS fliI NC_012947.1 1819410 1820783 R involved in type III protein export during flagellum assembly; flagellum-specific ATP synthase complement(1819410..1820783) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157694 YP_003035942.1 CDS fliH NC_012947.1 1820783 1821469 R binds to and inhibits the function of flagella specific ATPase FliI; flagellar assembly protein H complement(1820783..1821469) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157695 YP_003035943.1 CDS fliG NC_012947.1 1821462 1822457 R One of three proteins involved in switching the direction of the flagellar rotation; flagellar motor switch protein G complement(1821462..1822457) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157696 YP_003035944.1 CDS fliF NC_012947.1 1822450 1824108 R the MS-ring anchors the flagellum to the cytoplasmic membrane; part of the flagellar basal body which consists of four rings L, P, S, and M mounted on a central rod; flagellar MS-ring protein complement(1822450..1824108) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157697 YP_003035945.1 CDS fliE NC_012947.1 1824323 1824637 D forms a junction between the M-ring and FlgB during flagella biosynthesis; flagellar hook-basal body protein FliE 1824323..1824637 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157698 YP_003035946.1 CDS ECBD_1710 NC_012947.1 1826258 1826737 R PFAM: GCN5-related N-acetyltransferase; KEGG: efe:EFER_2671 acyltransferase; GCN5-related N-acetyltransferase complement(1826258..1826737) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157700 YP_003035947.1 CDS ECBD_1711 NC_012947.1 1826848 1827516 R PFAM: protein of unknown function DUF159; KEGG: ssn:SSON_1987 hypothetical protein; hypothetical protein complement(1826848..1827516) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157701 YP_003035948.1 CDS ECBD_1712 NC_012947.1 1827625 1827858 R PFAM: SirA family protein; KEGG: sea:SeAg_B1154 hypothetical protein; hypothetical protein complement(1827625..1827858) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157702 YP_003035949.1 CDS ECBD_1713 NC_012947.1 1827855 1829060 R PFAM: protein of unknown function DUF395 YeeE/YedE; inner membrane protein complement(1827855..1829060) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157703 YP_003035950.1 CDS ECBD_1714 NC_012947.1 1829247 1829660 D KEGG: sdy:SDY_1087 hypothetical protein; hypothetical protein 1829247..1829660 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157704 YP_003035951.1 CDS ECBD_1715 NC_012947.1 1829694 1831181 R converts 1,4-alpha-D-glucans to maltodextrin; cytoplasmic alpha-amylase complement(1829694..1831181) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157705 YP_003035952.1 CDS ECBD_1716 NC_012947.1 1831259 1831624 R binds flagellar cap subunits (FliD) and seems to prevents their oligomerization prior to its translocation through the flagellum-specific type III export pathway; acts as a repressor of flagellar class 2 operons by preventing FlhD2C2 to bind the promoter and inhibiting the FlhD2C2-dependent transcription; member of the type III cytoplasmic chaperone family; flagellar biosynthesis protein FliT complement(1831259..1831624) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157706 YP_003035953.1 CDS fliS NC_012947.1 1831624 1832034 R flagellin specific chaperone; flagellar protein FliS complement(1831624..1832034) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157707 YP_003035954.1 CDS fliD NC_012947.1 1832059 1833465 R involved in flagellin assembly; flagellar capping protein complement(1832059..1833465) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157708 YP_003035955.1 CDS ECBD_1719 NC_012947.1 1833731 1835227 D structural flagella protein; individual Salmonella serotypes usually alternate between the production of 2 antigenic forms of flagella, termed phase 1 and phase 2, each specified by separate structural genes; flagellin 1833731..1835227 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157709 YP_003035956.1 CDS fliA NC_012947.1 1835548 1836267 D sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; this sigma factor directs late flagellar biosynthesis genes; flagellar biosynthesis sigma factor 1835548..1836267 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157710 YP_003035957.1 CDS ECBD_1721 NC_012947.1 1836313 1836864 D expression activator of the class 2 type of flagellar operons, essential to achieve maximal cell motility; activator of type 1 fimbrial gene expression; cell density-responsive regulator; FliZ in Salmonella typhimurium induces HilA, an activator of invasion genes necessary to achieve full virulence; flagella biosynthesis protein FliZ 1836313..1836864 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157711 YP_003035958.1 CDS ECBD_1722 NC_012947.1 1836895 1837752 D PFAM: extracellular solute-binding protein family 3; SMART: extracellular solute-binding protein family 3; ionotropic glutamate receptor; KEGG: sfl:SF1963 cystine transporter subunit; cystine transporter subunit 1836895..1837752 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157712 YP_003035959.1 CDS ECBD_1723 NC_012947.1 1837857 1838843 D catalyzes the formation of pyruvate from D-cysteine; D-cysteine desulfhydrase 1837857..1838843 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157713 YP_003035960.1 CDS ECBD_1724 NC_012947.1 1838858 1839526 D TIGRFAM: polar amino acid ABC transporter inner membrane subunit; PFAM: binding-protein-dependent transporters inner membrane component; KEGG: ssn:SSON_1200 transporter permease (former YecC); polar amino acid ABC transporter permease 1838858..1839526 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157714 YP_003035961.1 CDS ECBD_1725 NC_012947.1 1839523 1840275 D PFAM: ABC transporter; SMART: ATPase AAA; KEGG: ssn:SSON_1201 amino-acid ABC transporter ATP-binding protein YecC; ABC transporter ATP-binding protein 1839523..1840275 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157715 YP_003035962.1 CDS ECBD_1726 NC_012947.1 1840505 1841227 D regulates genes involved in cell division; DNA-binding transcriptional activator SdiA 1840505..1841227 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157716 YP_003035963.1 CDS ECBD_1727 NC_012947.1 1841295 1841519 R KEGG: ssn:SSON_1203 hypothetical protein; hypothetical protein complement(1841295..1841519) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157717 YP_003035964.1 CDS ECBD_1728 NC_012947.1 1841506 1841682 R hypothetical protein complement(1841506..1841682) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157718 YP_003035965.1 CDS ECBD_1729 NC_012947.1 1841978 1842634 D in Escherichia coli the protein UvrY is part of a two-component system along with BarA that is needed for efficient switching between glycolytic and gluconeogenic carbon sources possibly by regulating the Csr system; in Salmonella SirA and BarA regulate virulence gene expression also via the Csr system; response regulator 1841978..1842634 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157719 YP_003035966.1 CDS uvrC NC_012947.1 1842631 1844463 D The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision; excinuclease ABC subunit C 1842631..1844463 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157720 YP_003035967.1 CDS ECBD_1731 NC_012947.1 1844520 1845068 D KEGG: sdy:SDY_1106 phosphatidylglycerophosphate synthetase; TIGRFAM: CDP-diacylglycerol/glycerol-3-phosphate 3-phosphatidyltransferase; PFAM: CDP-alcohol phosphatidyltransferase; phosphatidylglycerophosphate synthetase 1844520..1845068 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157721 YP_003035968.1 CDS ECBD_1732 NC_012947.1 1845718 1846383 D TIGRFAM: yecA family protein; PFAM: SEC-C motif domain protein; KEGG: sbo:SBO_1098 hypothetical protein; hypothetical protein 1845718..1846383 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157725 YP_003035969.1 CDS ECBD_1733 NC_012947.1 1846445 1847656 R TIGRFAM: aromatic amino acid transporter; PFAM: aromatic amino acid permease; Amino acid transporter transmembrane; KEGG: sfv:SFV_1951 tyrosine transporter; aromatic amino acid transporter complement(1846445..1847656) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157726 YP_003035970.1 CDS ECBD_1734 NC_012947.1 1847847 1848086 D KEGG: sbc:SbBS512_E1032 hypothetical protein; hypothetical protein 1847847..1848086 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157727 YP_003035971.1 CDS ECBD_1735 NC_012947.1 1848124 1848621 R cytoplasmic iron storage protein; ferritin complement(1848124..1848621) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157728 YP_003035972.1 CDS ECBD_1736 NC_012947.1 1848792 1849115 R KEGG: ssn:SSON_1214 hypothetical protein; hypothetical protein complement(1848792..1849115) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157729 YP_003035973.1 CDS ECBD_1737 NC_012947.1 1849321 1849467 R KEGG: sbc:SbBS512_E1028 hypothetical protein; hypothetical protein complement(1849321..1849467) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157730 YP_003035974.1 CDS ECBD_1738 NC_012947.1 1849579 1849830 D KEGG: sdy:SDY_1117 hypothetical protein; hypothetical protein 1849579..1849830 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157731 YP_003035975.1 CDS ECBD_1739 NC_012947.1 1849909 1850412 R PFAM: ferritin; KEGG: ssn:SSON_1216 ferritin-like protein; ferritin complement(1849909..1850412) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157732 YP_003035976.1 CDS ECBD_1740 NC_012947.1 1851209 1852198 D PFAM: periplasmic binding protein/LacI transcriptional regulator; periplasmic binding protein/LacI transcriptional regulator 1851209..1852198 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157733 YP_003035977.1 CDS araG NC_012947.1 1852268 1853782 D Part of the ABC transporter complex AraFGH involved in the high affinity transport of arabinose; L-arabinose transporter ATP-binding protein 1852268..1853782 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157734 YP_003035978.1 CDS araH NC_012947.1 1853797 1854783 D Part of the ABC transporter complex AraFGH involved in the high affinity transport of arabinose; L-arabinose transporter permease 1853797..1854783 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157735 YP_003035979.1 CDS ECBD_1743 NC_012947.1 1854950 1855750 D biosynthetic; catalyzes the formation of trehalose and phosphate from trehalose-6-phosphate; expression is increased under osmotic stress and induced during the transition to stationary phase and by decreased temperature; trehalose-6-phosphate phosphatase 1854950..1855750 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157736 YP_003035980.1 CDS ECBD_1744 NC_012947.1 1855725 1857149 D KEGG: ssn:SSON_1221 trehalose-6-phosphate synthase; TIGRFAM: alpha,alpha-trehalose-phosphate synthase [UDP-forming]; PFAM: glycosyl transferase family 20; trehalose-6-phosphate synthase 1855725..1857149 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157737 YP_003035981.1 CDS ECBD_1745 NC_012947.1 1857156 1857584 R ppGpp-dependent; membrane-associated, stress protein produced under conditions of nutrient deprivation, osmotic shock and oxidative stress; universal stress protein UspC complement(1857156..1857584) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157738 YP_003035982.1 CDS ECBD_1746 NC_012947.1 1858364 1858714 D with FlhC is involved in the activation of class 2 flagellar genes and is involved in the regulation of a number of other genetic systems; transcriptional activator FlhD 1858364..1858714 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157739 YP_003035983.1 CDS ECBD_1747 NC_012947.1 1858717 1859295 D With FlhD is involved in the activation of class 2 flagellar genes and as well as a number of other genetic systems; transcriptional activator FlhC 1858717..1859295 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157740 YP_003035984.1 CDS ECBD_1748 NC_012947.1 1859422 1860309 D With MotB forms the ion channels that couple flagellar rotation to proton/sodium motive force across the membrane and forms the stator elements of the rotary flagellar machine; flagellar motor protein MotA 1859422..1860309 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157741 YP_003035985.1 CDS motB NC_012947.1 1860306 1861232 D with MotA forms the ion channels that couple flagellar rotation to proton/sodium motive force across the membrane and forms the stator elements of the rotary flagellar machine; flagellar motor protein MotB 1860306..1861232 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157742 YP_003035986.1 CDS ECBD_1750 NC_012947.1 1861237 1863201 D chemotactic sensory histidine kinase in two-component regulatory system with CheB and CheY; sensory histidine kinase/signal sensing protein; CheA is the histidine kinase component; chemotaxis protein CheA 1861237..1863201 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157743 YP_003035987.1 CDS ECBD_1751 NC_012947.1 1863222 1863725 D PFAM: CheW domain protein; SMART: CheW domain protein; KEGG: ssn:SSON_1230 purine-binding chemotaxis protein; purine-binding chemotaxis protein 1863222..1863725 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157744 YP_003035988.1 CDS ECBD_1752 NC_012947.1 1863870 1865531 D PFAM: chemotaxis sensory transducer; ligand binding Tar domain protein; histidine kinase HAMP region domain protein; SMART: chemotaxis sensory transducer; histidine kinase HAMP region domain protein; ligand binding Tar domain protein; KEGG: ssn:SSON_1231 methyl-accepting chemotaxis protein II; methyl-accepting chemotaxis sensory transducer 1863870..1865531 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158162 YP_003035989.1 CDS ECBD_1753 NC_012947.1 1865577 1867178 D mediates taxis toward dipeptides; methyl-accepting protein IV 1865577..1867178 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158163 YP_003035990.1 CDS ECBD_1754 NC_012947.1 1867197 1868057 D methylates the MCP; chemotaxis methyltransferase CheR 1867197..1868057 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158164 YP_003035991.1 CDS ECBD_1755 NC_012947.1 1868060 1869109 D regulates chemotaxis by demethylation of methyl-accepting chemotaxis proteins; chemotaxis-specific methylesterase 1868060..1869109 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158165 YP_003035992.1 CDS ECBD_1756 NC_012947.1 1869124 1869513 D chemotaxis regulator that, when phosphorylated, interacts with the flagellar motor causing the flagella to spin clockwise which causes the cell to tumble; chemotaxis regulatory protein CheY 1869124..1869513 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158166 YP_003035993.1 CDS ECBD_1757 NC_012947.1 1869524 1870168 D cytosolic phosphatase which functions in the chemotaxis signal transduction complex by controlling the level of phosphorylated CheY through dephosphorylation; chemotaxis regulator CheZ 1869524..1870168 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158167 YP_003035994.1 CDS flhB NC_012947.1 1870370 1871518 D membrane protein responsible for substrate specificity switching from rod/hook-type export to filament-type export; flagellar biosynthesis protein FlhB 1870370..1871518 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158168 YP_003035995.1 CDS flhA NC_012947.1 1871511 1873589 D membrane protein involved in the flagellar export apparatus; flagellar biosynthesis protein FlhA 1871511..1873589 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158169 YP_003035996.1 CDS ECBD_1760 NC_012947.1 1873589 1873981 D PFAM: flagellar FlhE family protein; KEGG: sbc:SbBS512_E2171 flagellar protein FlhE; flagellar FlhE family protein 1873589..1873981 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158170 YP_003035997.1 CDS ECBD_1761 NC_012947.1 1874101 1874589 R PFAM: protein of unknown function DUF1311; KEGG: sbo:SBO_1123 hypothetical protein; hypothetical protein complement(1874101..1874589) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158171 YP_003035998.1 CDS argS NC_012947.1 1874766 1876499 R catalyzes a two-step reaction, first charging an arginine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA synthetase; arginyl-tRNA synthetase complement(1874766..1876499) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158172 YP_003035999.1 CDS ECBD_1763 NC_012947.1 1876715 1877281 D PFAM: protein of unknown function DUF991; hypothetical protein 1876715..1877281 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158173 YP_003036000.1 CDS ECBD_1764 NC_012947.1 1877295 1878041 D PFAM: CutC family protein; KEGG: elf:LF82_0392 copper homeostasis protein CutC; copper homeostasis protein CutC 1877295..1878041 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158174 YP_003036001.1 CDS ECBD_1765 NC_012947.1 1878429 1879529 D PFAM: NapC/NirT cytochrome C domain protein; KEGG: sfv:SFV_1911 cytochrome C protein; NapC/NirT cytochrome C domain protein 1878429..1879529 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158175 YP_003036002.1 CDS ECBD_1766 NC_012947.1 1879554 1881983 D TIGRFAM: molybdopterin guanine dinucleotide-containing S/N-oxide reductase; PFAM: molybdopterin oxidoreductase; molydopterin dinucleotide-binding region; molybdopterin guanine dinucleotide-containing S/N-oxide reductase 1879554..1881983 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158176 YP_003036003.1 CDS ECBD_1767 NC_012947.1 1882148 1883119 R TIGRFAM: methyltransferase; PFAM: methyltransferase; methyltransferase complement(1882148..1883119) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158177 YP_003036004.1 CDS ECBD_1768 NC_012947.1 1883116 1883859 R TIGRFAM: methyltransferase; PFAM: methyltransferase type 12; methyltransferase type 11; KEGG: sdy:SDY_1169 hypothetical protein; methyltransferase complement(1883116..1883859) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158178 YP_003036005.1 CDS ECBD_1769 NC_012947.1 1883900 1884295 R KEGG: sdy:SDY_1168 hypothetical protein; hypothetical protein complement(1883900..1884295) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158179 YP_003036006.1 CDS ECBD_1770 NC_012947.1 1884348 1885166 R PFAM: protein of unknown function DUF72; hypothetical protein complement(1884348..1885166) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158180 YP_003036007.1 CDS ECBD_1771 NC_012947.1 1885163 1885729 R PFAM: isochorismatase hydrolase; KEGG: elf:LF82_2940 isochorismatase family protein YecD; hypothetical protein complement(1885163..1885729) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158181 YP_003036008.1 CDS aspS NC_012947.1 1886039 1887811 D catalyzes a two-step reaction, first charging an aspartate molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; contains discriminating and non-discriminating subtypes; aspartyl-tRNA synthetase 1886039..1887811 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158182 YP_003036009.1 CDS ntpA NC_012947.1 1887872 1888381 D converts dATP to dAMP and pyrophosphate; dATP pyrophosphohydrolase 1887872..1888381 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158183 YP_003036010.1 CDS ECBD_1774 NC_012947.1 1888410 1889150 D PFAM: protein of unknown function DUF28; KEGG: efe:EFER_1208 hypothetical protein; hypothetical protein 1888410..1889150 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158184 YP_003036011.1 CDS ruvC NC_012947.1 1889185 1889706 D endonuclease; resolves Holliday structures; forms a complex of RuvABC; the junction binding protein RuvA forms a hexameric ring along with the RuvB helicase and catalyzes branch migration; RuvC then interacts with RuvAB to resolve the Holliday junction by nicking DNA strands of like polarity; Holliday junction resolvase 1889185..1889706 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158185 YP_003036012.1 CDS ECBD_1776 NC_012947.1 1889708 1890310 R PFAM: protein of unknown function DUF1105; KEGG: ssn:SSON_1279 hypothetical protein; hypothetical protein complement(1889708..1890310) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158186 YP_003036013.1 CDS ruvA NC_012947.1 1890585 1891196 D plays an essential role in ATP-dependent branch migration of the Holliday junction; Holliday junction DNA helicase RuvA 1890585..1891196 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158187 YP_003036014.1 CDS ruvB NC_012947.1 1891205 1892215 D promotes strand exchange during homologous recombination; RuvAB complex promotes branch migration; RuvABC complex scans the DNA during branch migration and resolves Holliday junctions at consensus sequences; forms hexameric rings around opposite DNA arms; requires ATP for branch migration and orientation of RuvAB complex determines direction of migration; Holliday junction DNA helicase RuvB 1891205..1892215 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158188 YP_003036015.1 CDS znuB NC_012947.1 1892556 1893341 R involved in transport of zinc(II) with ZnuA and C; high-affinity zinc transporter membrane component complement(1892556..1893341) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158189 YP_003036016.1 CDS znuC NC_012947.1 1893338 1894093 R involved in transport of zinc(II) with ZnuA and C; high-affinity zinc transporter ATPase complement(1893338..1894093) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158190 YP_003036017.1 CDS znuA NC_012947.1 1894157 1895104 D involved in transport of zinc(II) with ZnuA and C; high-affinity zinc transporter periplasmic component 1894157..1895104 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158191 YP_003036018.1 CDS ECBD_1782 NC_012947.1 1895120 1896442 D PFAM: peptidase M23; peptidoglycan-binding LysM; Opacity-associated protein A; Opacity-associated protein A domain protein; SMART: peptidoglycan-binding LysM; KEGG: sfx:S1932 hypothetical protein; hypothetical protein 1895120..1896442 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158192 YP_003036019.1 CDS ECBD_1783 NC_012947.1 1896562 1897533 D transfers myristate or laurate, activated on ACP, to the lipid IVA moiety of (KDO)2-(lauroyl)-lipid IVA; lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase 1896562..1897533 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158193 YP_003036020.1 CDS ECBD_1784 NC_012947.1 1897664 1899106 R catalyzes the formation of phosphoenolpyruvate from pyruvate; pyruvate kinase complement(1897664..1899106) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158194 YP_003036021.1 CDS ECBD_1785 NC_012947.1 1899234 1900103 R Represses the expression of the zwf, eda, glp and gap; DNA-binding transcriptional regulator HexR complement(1899234..1900103) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158195 YP_003036022.1 CDS ECBD_1786 NC_012947.1 1900126 1900275 D KEGG: sbc:SbBS512_E2128 hypothetical protein; hypothetical protein 1900126..1900275 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158196 YP_003036023.1 CDS ECBD_1787 NC_012947.1 1900441 1901916 D catalyzes the formation of D-glucono-1,5-lactone 6-phosphate from D-glucose 6-phosphate; glucose-6-phosphate 1-dehydrogenase 1900441..1901916 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158197 YP_003036024.1 CDS ECBD_1788 NC_012947.1 1902151 1903962 D catalyzes the formation of 2-dehydro-3-deoxy-6-phospho-D-gluconate from 6-phospho-D-gluconate; phosphogluconate dehydratase 1902151..1903962 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158198 YP_003036025.1 CDS ECBD_1789 NC_012947.1 1903999 1904640 D catalyzes the formation of pyruvate and glyoxylate from 4-hydroxy-2-oxoglutarate; or pyruvate and D-glyceraldehyde 3-phosphate from 2-dehydro-3-deoxy-D-glyconate 6-phosphate; keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase 1903999..1904640 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158199 YP_003036026.1 CDS purT NC_012947.1 1904696 1905874 R non-folate utilizing enzyme, catalyzes the production of beta-formyl glycinamide ribonucleotide from formate, ATP, and beta-GAR and a side reaction producing acetyl phosphate and ADP from acetate and ATP; involved in de novo purine biosynthesis; phosphoribosylglycinamide formyltransferase 2 complement(1904696..1905874) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158200 YP_003036027.1 CDS ECBD_1791 NC_012947.1 1906008 1906298 D PFAM: YebG family protein; KEGG: sdy:SDY_1134 DNA damage-inducible protein YebG; DNA damage-inducible protein YebG 1906008..1906298 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158201 YP_003036028.1 CDS ECBD_1792 NC_012947.1 1906365 1906721 D secreted protein; unknown function; hypothetical protein 1906365..1906721 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158202 YP_003036029.1 CDS ECBD_1793 NC_012947.1 1906878 1906988 R hypothetical protein complement(1906878..1906988) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158203 YP_003036030.1 CDS ECBD_1794 NC_012947.1 1907048 1907707 D PFAM: protein of unknown function DUF533; KEGG: ssn:SSON_1302 hypothetical protein; hypothetical protein 1907048..1907707 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158204 YP_003036031.1 CDS ECBD_1795 NC_012947.1 1907916 1909976 D PtrB; oligopeptidase that cleaves peptide bonds following arginine and lysine residues; protease 2 1907916..1909976 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158205 YP_003036032.1 CDS ECBD_1796 NC_012947.1 1909973 1910635 R 3'-5' exonuclease activity on single or double-strand DNA; exodeoxyribonuclease X complement(1909973..1910635) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158206 YP_003036033.1 CDS ECBD_1797 NC_012947.1 1910659 1911315 R PFAM: nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase; KEGG: sbc:SbBS512_E2118 hydrolase, carbon-nitrogen family; nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase complement(1910659..1911315) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158207 YP_003036034.1 CDS ECBD_1798 NC_012947.1 1911417 1911647 R PFAM: DNA polymerase II subunit beta; KEGG: sdy:SDY_1128 DNA polymerase III subunit theta; DNA polymerase III subunit theta complement(1911417..1911647) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158208 YP_003036035.1 CDS ECBD_1799 NC_012947.1 1911786 1912160 D PFAM: copper resistance protein CopC; KEGG: sbc:SbBS512_E2116 copper resistance protein CopC; hypothetical protein 1911786..1912160 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158209 YP_003036036.1 CDS ECBD_1800 NC_012947.1 1912164 1913036 D PFAM: copper resistance D domain protein; KEGG: sbc:SbBS512_E2115 copper resistance protein D; copper resistance D domain protein 1912164..1913036 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158210 YP_003036037.1 CDS ECBD_1801 NC_012947.1 1913049 1913390 D KEGG: sdy:SDY_1125 hypothetical protein; hypothetical protein 1913049..1913390 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158211 YP_003036038.1 CDS pphA NC_012947.1 1913785 1914441 D Plays a key role in signaling protein misfolding via the cpxR/cpxA transducing system; modulates the phosphorylated status of many phosphoproteins; serine/threonine protein phosphatase 1 1913785..1914441 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158212 YP_003036039.1 CDS ECBD_1803 NC_012947.1 1914442 1914717 R PFAM: protein of unknown function DUF1482; KEGG: ssn:SSON_1324 hypothetical protein; hypothetical protein complement(1914442..1914717) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158213 YP_003036040.1 CDS ECBD_1804 NC_012947.1 1914738 1914974 R PFAM: protein of unknown function DUF1480; KEGG: sfv:SFV_1392 hypothetical protein; hypothetical protein complement(1914738..1914974) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158214 YP_003036041.1 CDS yebU NC_012947.1 1915092 1916537 R in Escherichia coli this enzyme specifically methylates C1407 of the 16S rRNA; rRNA (cytosine-C(5)-)-methyltransferase RsmF complement(1915092..1916537) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158215 YP_003036042.1 CDS ECBD_1806 NC_012947.1 1916611 1919244 R PFAM: hypothetical protein; KEGG: ssn:SSON_1327 hypothetical protein; hypothetical protein complement(1916611..1919244) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158216 YP_003036043.1 CDS ECBD_1807 NC_012947.1 1919213 1920496 R TIGRFAM: integral membrane protein, PqiA family; PFAM: paraquat-inducible protein A; KEGG: sbo:SBO_1248 hypothetical protein; integral membrane protein, PqiA family complement(1919213..1920496) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158217 YP_003036044.1 CDS ECBD_1808 NC_012947.1 1920572 1921123 D PFAM: GAF domain protein; SMART: GAF domain protein; KEGG: sdy:SDY_1980 hypothetical protein; GAF sensor protein 1920572..1921123 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158218 YP_003036045.1 CDS ECBD_1809 NC_012947.1 1921220 1921918 D affects solute and DNA transport through an unknown mechanism; solute/DNA competence effector 1921220..1921918 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158219 YP_003036046.1 CDS ECBD_1810 NC_012947.1 1921938 1923986 D Involved in the cleavage of a C-terminal peptide of 11 residues from the precursor form of penicillin-binding protein 3; carboxy-terminal protease 1921938..1923986 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158220 YP_003036047.1 CDS ECBD_1811 NC_012947.1 1924178 1925059 D metalloprotease; heat shock protein HtpX 1924178..1925059 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158221 YP_003036048.1 CDS ECBD_1812 NC_012947.1 1925105 1926478 R PFAM: major facilitator superfamily MFS_1; KEGG: sbc:SbBS512_E2096 transporter major facilitator family; major facilitator superfamily MFS_1 complement(1925105..1926478) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158222 YP_003036049.1 CDS ECBD_1813 NC_012947.1 1926655 1927446 D PFAM: transcriptional regulator IclR ; regulatory protein IclR; SMART: regulatory protein IclR; KEGG: sbc:SbBS512_E2095 transcriptional regulator KdgR; transcriptional regulator, IclR family 1926655..1927446 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158223 YP_003036050.1 CDS ECBD_1814 NC_012947.1 1927589 1927828 R KEGG: sdy:SDY_1974 hypothetical protein; hypothetical protein complement(1927589..1927828) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158224 YP_003036051.1 CDS ECBD_1815 NC_012947.1 1927987 1928130 D KEGG: sdy:SDY_1973 hypothetical protein; hypothetical protein 1927987..1928130 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158225 YP_003036052.1 CDS ECBD_1816 NC_012947.1 1928205 1928492 D KEGG: elf:LF82_2929 uncharacterized protein YebO; hypothetical protein 1928205..1928492 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158226 YP_003036053.1 CDS ECBD_1817 NC_012947.1 1929161 1929304 D KEGG: sdy:SDY_1970 hypothetical protein; hypothetical protein 1929161..1929304 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158227 YP_003036054.1 CDS ECBD_1818 NC_012947.1 1929317 1929526 D PFAM: Cold-shock protein DNA-binding; SMART: Cold shock protein; KEGG: sed:SeD_A1478 cold shock protein CspC; cold shock-like protein CspC 1929317..1929526 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158228 YP_003036055.1 CDS rrmA NC_012947.1 1929692 1930501 D methylates the guanosine in position 745 of 23S rRNA; required for translation and cell growth; 23S rRNA methyltransferase A 1929692..1930501 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158229 YP_003036056.1 CDS ECBD_1820 NC_012947.1 1930498 1931064 R membrane protein YebN; hypothetical protein complement(1930498..1931064) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158230 YP_003036057.1 CDS ECBD_1821 NC_012947.1 1931493 1931951 R PFAM: protein of unknown function DUF986; KEGG: ssn:SSON_1340 hypothetical protein; hypothetical protein complement(1931493..1931951) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158231 YP_003036058.1 CDS ECBD_1822 NC_012947.1 1932006 1932857 R hosphoenolpyruvate-dependent sugar phosphotransferase system catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane; IID with IIC forms the translocation channel; PTS system mannose-specific transporter subunit IID complement(1932006..1932857) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158232 YP_003036059.1 CDS ECBD_1823 NC_012947.1 1932870 1933670 R TIGRFAM: PTS system, mannose/fructose/sorbose family, IIC subunit; PFAM: phosphotransferase system PTS sorbose-specific IIC subunit; KEGG: sbc:SbBS512_E2084 PTS system, mannose-specific IIC component; PTS system, mannose/fructose/sorbose family, IIC subunit complement(1932870..1933670) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158233 YP_003036060.1 CDS ECBD_1824 NC_012947.1 1933733 1934704 R KEGG: ssn:SSON_1343 PTS enzyme IIAB, mannose-specific; TIGRFAM: PTS system, mannose/fructose/sorbose family, IIA subunit; PTS system, mannose/fructose/sorbose family, IIB subunit; PFAM: PTS system sorbose subfamily IIB component; PTS system fructose subfamily IIA component; PTS system, mannose/fructose/sorbose family, IIA subunit complement(1933733..1934704) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158234 YP_003036061.1 CDS ECBD_1825 NC_012947.1 1934970 1935179 D KEGG: stm:STM1829 cytoplasmic protein; cytoplasmic protein 1934970..1935179 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158235 YP_003036062.1 CDS ECBD_1826 NC_012947.1 1935167 1936723 D PFAM: integral membrane protein TerC; CBS domain containing protein; transporter-associated region; integral membrane protein TerC 1935167..1936723 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158236 YP_003036063.1 CDS ECBD_1827 NC_012947.1 1936727 1938325 R PFAM: EAL domain protein; SMART: EAL domain protein; KEGG: ssn:SSON_1345 hypothetical protein; diguanylate phosphodiesterase complement(1936727..1938325) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158237 YP_003036064.1 CDS ECBD_1828 NC_012947.1 1938456 1939820 R KEGG: sbo:SBO_1270 L-serine deaminase; TIGRFAM: L-serine dehydratase 1; PFAM: serine dehydratase alpha chain; serine dehydratase beta chain; L-serine dehydratase complement(1938456..1939820) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158238 YP_003036065.1 CDS ECBD_1829 NC_012947.1 1940004 1940582 R PFAM: NUDIX hydrolase; KEGG: sdy:SDY_1719 hypothetical protein; hypothetical protein complement(1940004..1940582) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158239 YP_003036066.1 CDS ECBD_1830 NC_012947.1 1940586 1941947 R TIGRFAM: para-aminobenzoate synthase, subunit I; PFAM: chorismate binding-like; anthranilate synthase component I domain protein; KEGG: ssn:SSON_1348 p-aminobenzoate synthetase, component I; para-aminobenzoate synthase, subunit I complement(1940586..1941947) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158240 YP_003036067.1 CDS ECBD_1831 NC_012947.1 1942021 1942200 D KEGG: ssn:SSON_1349 hypothetical protein; hypothetical protein 1942021..1942200 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158995 YP_003036068.1 CDS ECBD_1832 NC_012947.1 1942320 1942643 R KEGG: sfv:SFV_1419 hypothetical protein; hypothetical protein complement(1942320..1942643) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158909 YP_003036069.1 CDS ECBD_1833 NC_012947.1 1943042 1943389 R KEGG: ssn:SSON_1352 hypothetical protein; hypothetical protein complement(1943042..1943389) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158910 YP_003036070.1 CDS ECBD_1834 NC_012947.1 1943518 1945428 D KEGG: sbo:SBO_1280 enzyme; enzyme 1943518..1945428 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158911 YP_003036071.1 CDS ECBD_1835 NC_012947.1 1945486 1946181 D KEGG: sfv:SFV_1422 hypothetical protein; hypothetical protein 1945486..1946181 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158912 YP_003036072.1 CDS ECBD_1836 NC_012947.1 1946221 1946802 D TIGRFAM: outer membrane lipoprotein, Slp family; KEGG: sfv:SFV_1423 outer membrane protein; outer membrane lipoprotein, Slp family 1946221..1946802 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158913 YP_003036073.1 CDS ECBD_1837 NC_012947.1 1946941 1948692 D Activates fatty acids by binding to coenzyme A; long-chain-fatty-acid--CoA ligase 1946941..1948692 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158914 YP_003036074.1 CDS ECBD_1838 NC_012947.1 1948774 1949889 D TIGRFAM: ribonuclease D; ribonuclease D 1948774..1949889 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158915 YP_003036075.1 CDS ECBD_1839 NC_012947.1 1949943 1950908 R KEGG: sbo:SBO_1285 diogenase subunit beta; diogenase subunit beta complement(1949943..1950908) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158916 YP_003036076.1 CDS ECBD_1840 NC_012947.1 1950964 1952088 R KEGG: ssn:SSON_1359 hypothetical protein; hypothetical protein complement(1950964..1952088) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158917 YP_003036077.1 CDS ECBD_1842 NC_012947.1 1953756 1954841 R TIGRFAM: tartrate dehydrogenase; PFAM: isocitrate/isopropylmalate dehydrogenase; tartrate dehydrogenase complement(1953756..1954841) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158919 YP_003036078.1 CDS ECBD_1843 NC_012947.1 1954944 1955867 D PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: sbo:SBO_1289 transcriptional regulator LysR-type; LysR family transcriptional regulator 1954944..1955867 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158920 YP_003036079.1 CDS ECBD_1844 NC_012947.1 1955994 1956632 D PFAM: lysine exporter (LYSE/YGGA); KEGG: ssn:SSON_1363 leucine export protein LeuE; leucine export protein LeuE 1955994..1956632 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158921 YP_003036080.1 CDS ECBD_1847 NC_012947.1 1958289 1958513 D PFAM: protein of unknown function DUF1971; KEGG: sdy:SDY_1697 hypothetical protein; hypothetical protein 1958289..1958513 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158924 YP_003036081.1 CDS ECBD_1848 NC_012947.1 1958517 1958699 D PFAM: Domain of unknown function DUF1869; KEGG: ssn:SSON_1365 hypothetical protein; hypothetical protein 1958517..1958699 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158925 YP_003036082.1 CDS ECBD_1849 NC_012947.1 1958716 1958835 D hypothetical protein 1958716..1958835 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158926 YP_003036083.1 CDS ECBD_1850 NC_012947.1 1958847 1959095 D PFAM: transglycosylase; KEGG: sdy:SDY_1695 hypothetical protein; hypothetical protein 1958847..1959095 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158927 YP_003036084.1 CDS ECBD_1851 NC_012947.1 1959362 1960387 R KEGG: sdy:SDY_1692 hypothetical protein; TIGRFAM: diguanylate cyclase; PFAM: GGDEF domain containing protein; GAF domain protein; SMART: GGDEF domain containing protein; GAF domain protein; diguanylate cyclase with GAF sensor complement(1959362..1960387) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158928 YP_003036085.1 CDS ECBD_1852 NC_012947.1 1960570 1960824 D PFAM: protein of unknown function DUF333; KEGG: sbc:SbBS512_E2046 lipoprotein; hypothetical protein 1960570..1960824 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158929 YP_003036086.1 CDS ECBD_1853 NC_012947.1 1960846 1961193 R PFAM: protein of unknown function DUF488; KEGG: ssn:SSON_1369 hypothetical protein; hypothetical protein complement(1960846..1961193) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158930 YP_003036087.1 CDS ECBD_1854 NC_012947.1 1961248 1962429 R TIGRFAM: cyanate transporter; PFAM: major facilitator superfamily MFS_1; KEGG: sbo:SBO_1302 amino acid/amine transport protein; cyanate transporter complement(1961248..1962429) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158931 YP_003036088.1 CDS ECBD_1855 NC_012947.1 1962529 1963347 D PFAM: helix-turn-helix- domain containing protein AraC type; SMART: helix-turn-helix- domain containing protein AraC type; KEGG: sdy:SDY_1688 AraC-type regulatory protein; AraC family transcriptional regulator 1962529..1963347 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158932 YP_003036089.1 CDS ECBD_1856 NC_012947.1 1963304 1963750 R PFAM: protein of unknown function DUF441; KEGG: sbo:SBO_1304 hypothetical protein; hypothetical protein complement(1963304..1963750) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158933 YP_003036090.1 CDS ECBD_1857 NC_012947.1 1964025 1964528 R PFAM: YbaK/prolyl-tRNA synthetase associated region; KEGG: elf:LF82_2911 uncharacterized protein YeaK; YbaK/prolyl-tRNA synthetase associated region complement(1964025..1964528) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158934 YP_003036091.1 CDS ECBD_1858 NC_012947.1 1964571 1966061 R KEGG: elf:LF82_2910 uncharacterized protein YeaJ; TIGRFAM: diguanylate cyclase; PFAM: GGDEF domain containing protein; SMART: GGDEF domain containing protein; diguanylate cyclase complement(1964571..1966061) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158935 YP_003036092.1 CDS ECBD_1859 NC_012947.1 1966242 1967717 R KEGG: ssn:SSON_1377 hypothetical protein; TIGRFAM: diguanylate cyclase; PFAM: GGDEF domain containing protein; SMART: GGDEF domain containing protein; diguanylate cyclase complement(1966242..1967717) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158936 YP_003036093.1 CDS ECBD_1860 NC_012947.1 1967864 1969147 R PFAM: protein of unknown function DUF444; KEGG: sbo:SBO_1309 hypothetical protein; hypothetical protein complement(1967864..1969147) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158937 YP_003036094.1 CDS ECBD_1862 NC_012947.1 1971630 1972376 D PFAM: MltA-interacting MipA family protein; KEGG: ssn:SSON_1380 hypothetical protein; MltA-interacting MipA family protein 1971630..1972376 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158939 YP_003036095.1 CDS ECBD_1863 NC_012947.1 1972466 1973320 D PFAM: aldo/keto reductase; aldo/keto reductase 1972466..1973320 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158940 YP_003036096.1 CDS ECBD_1864 NC_012947.1 1973368 1974252 R PFAM: aldose 1-epimerase; KEGG: sfv:SFV_1435 hypothetical protein; aldose 1-epimerase complement(1973368..1974252) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158941 YP_003036097.1 CDS ECBD_1865 NC_012947.1 1974336 1975331 R KEGG: ssn:SSON_1384 glyceraldehyde-3-phosphate dehydrogenase A; TIGRFAM: glyceraldehyde-3-phosphate dehydrogenase, type I; PFAM: glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase complement(1974336..1975331) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158942 YP_003036098.1 CDS ECBD_1866 NC_012947.1 1975673 1976086 D this stereospecific enzymes reduces the R isomer of methionine sulfoxide while MsrA reduces the S form; provides protection against oxidative stress; methionine sulfoxide reductase B 1975673..1976086 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158943 YP_003036099.1 CDS ECBD_1867 NC_012947.1 1976128 1976400 D PFAM: protein of unknown function DUF1315; KEGG: sfv:SFV_1438 hypothetical protein; hypothetical protein 1976128..1976400 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158944 YP_003036100.1 CDS ECBD_1868 NC_012947.1 1976770 1977846 D PFAM: alcohol dehydrogenase zinc-binding domain protein; alcohol dehydrogenase GroES domain protein; KEGG: elf:LF82_2898 uncharacterized zinc-type alcohol dehydrogenase-like; alcohol dehydrogenase zinc-binding domain protein 1976770..1977846 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158945 YP_003036101.1 CDS ECBD_1869 NC_012947.1 1977873 1979252 D PFAM: major facilitator superfamily MFS_1; general substrate transporter; KEGG: sdy:SDY_1492 transporter; major facilitator superfamily MFS_1 1977873..1979252 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158946 YP_003036102.1 CDS ECBD_1870 NC_012947.1 1979269 1980312 D PFAM: alcohol dehydrogenase zinc-binding domain protein; alcohol dehydrogenase GroES domain protein; KEGG: elf:LF82_2896 uncharacterized zinc-type alcohol dehydrogenase-like; alcohol dehydrogenase zinc-binding domain protein 1979269..1980312 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158947 YP_003036103.1 CDS ECBD_1871 NC_012947.1 1980333 1981169 D TIGRFAM: ketose-bisphosphate aldolase; PFAM: ketose-bisphosphate aldolase class-II; ketose-bisphosphate aldolase 1980333..1981169 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158948 YP_003036104.1 CDS ECBD_1872 NC_012947.1 1981174 1982121 D PFAM: PfkB domain protein; PfkB domain protein 1981174..1982121 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158949 YP_003036105.1 CDS ECBD_1873 NC_012947.1 1982131 1983111 D PFAM: aldo/keto reductase; KEGG: sfv:SFV_1444 hypothetical protein; aldo/keto reductase 1982131..1983111 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158950 YP_003036106.1 CDS ECBD_1874 NC_012947.1 1983248 1984006 D PFAM: regulatory protein DeoR; SMART: regulatory protein DeoR; KEGG: elf:LF82_2892 uncharacterized HTH-type transcriptional regulator; DeoR family transcriptional regulator 1983248..1984006 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158951 YP_003036107.1 CDS ECBD_1875 NC_012947.1 1984124 1985482 D PFAM: major facilitator superfamily MFS_1; general substrate transporter; KEGG: elf:LF82_2891 inner membrane metabolite transport protein YdjE; major facilitator superfamily MFS_1 1984124..1985482 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158952 YP_003036108.1 CDS ECBD_1876 NC_012947.1 1985575 1986216 R catalyzes the formation of nicotinate from nicotinamide in NAD biosynthesis and the formation of pyrazinoate from pyrazinamide; nicotinamidase/pyrazinamidase complement(1985575..1986216) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158953 YP_003036109.1 CDS ansA NC_012947.1 1986227 1987243 R converts asparagine to aspartate and ammonia; cytoplasmic asparaginase I complement(1986227..1987243) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158954 YP_003036110.1 CDS ECBD_1878 NC_012947.1 1987410 1989266 R SppA; catalyzes the degradation of cleaved signal peptides; essential to maintain secretion of mature proteins across the membrane; protease 4 complement(1987410..1989266) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158955 YP_003036111.1 CDS ECBD_1879 NC_012947.1 1989427 1989978 D PFAM: nitroreductase; KEGG: sbo:SBO_1322 hypothetical protein; hypothetical protein 1989427..1989978 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158956 YP_003036112.1 CDS ECBD_1880 NC_012947.1 1990095 1991138 D catalyzes the formation of selenophosphate from selenide and ATP; selenophosphate synthetase 1990095..1991138 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158957 YP_003036113.1 CDS ECBD_1881 NC_012947.1 1991143 1993104 D decatenates replicating daughter chromosomes; DNA topoisomerase III 1991143..1993104 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158958 YP_003036114.1 CDS ECBD_1882 NC_012947.1 1993232 1994272 D KEGG: sfl:SF1461 hypothetical protein; hypothetical protein 1993232..1994272 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158959 YP_003036115.1 CDS ECBD_1883 NC_012947.1 1994389 1995732 R converts 2-oxoglutarate to glutamate; in Escherichia coli this enzyme plays a role in glutamate synthesis when the cell is under energy restriction; uses NADPH; forms a homohexamer; glutamate dehydrogenase complement(1994389..1995732) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158960 YP_003036116.1 CDS ECBD_1884 NC_012947.1 1995968 1996240 D PFAM: protein of unknown function DUF1496; KEGG: sbc:SbBS512_E2012 hypothetical protein; hypothetical protein 1995968..1996240 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158961 YP_003036117.1 CDS ECBD_1885 NC_012947.1 1996206 1996613 R PFAM: NUDIX hydrolase; KEGG: elf:LF82_1533 CTP pyrophosphohydrolase; pyrimidine (deoxy)nucleoside triphosphate pyrophosphohydrolase complement(1996206..1996613) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158962 YP_003036118.1 CDS ECBD_1886 NC_012947.1 1996700 1997320 D PFAM: CDP-alcohol phosphatidyltransferase; KEGG: sfx:S1582 cytochrome oxidase; CDP-alcohol phosphatidyltransferase 1996700..1997320 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158963 YP_003036119.1 CDS ECBD_1887 NC_012947.1 1997329 1998636 R PFAM: rhodanese; SMART: Rhodanese domain protein; KEGG: sbo:SBO_1332 thiosulfate sulfur transferase; rhodanese complement(1997329..1998636) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158964 YP_003036120.1 CDS ECBD_1888 NC_012947.1 1998703 1999356 R PFAM: ABC transporter; SMART: ATPase AAA; KEGG: sfv:SFV_1462 ATP-binding component of a transporter; ABC transporter complement(1998703..1999356) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158965 YP_003036121.1 CDS ECBD_1889 NC_012947.1 1999356 2000891 R KEGG: sbc:SbBS512_E2004 ABC transporter permease protein; ABC transporter permease complement(1999356..2000891) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158966 YP_003036122.1 CDS ECBD_1890 NC_012947.1 2000864 2002030 R PFAM: extracellular solute-binding protein family 1; KEGG: ssn:SSON_1403 hypothetical protein; ABC transporter substrate-binding protein complement(2000864..2002030) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158967 YP_003036123.1 CDS ECBD_1891 NC_012947.1 2002040 2002588 R PFAM: carboxymuconolactone decarboxylase; KEGG: sbc:SbBS512_E2002 carboxymuconolactone decarboxylase family protein; carboxymuconolactone decarboxylase complement(2002040..2002588) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158968 YP_003036124.1 CDS ECBD_1892 NC_012947.1 2002588 2003295 R PFAM: SNARE associated Golgi protein; KEGG: elf:LF82_2903 UPF0043 inner membrane protein YdjZ; hypothetical protein complement(2002588..2003295) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158969 YP_003036125.1 CDS ECBD_1893 NC_012947.1 2003310 2003927 R KEGG: ssn:SSON_1406 hypothetical protein; hypothetical protein complement(2003310..2003927) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158970 YP_003036126.1 CDS ECBD_1894 NC_012947.1 2003955 2004665 R PFAM: SNARE associated Golgi protein; KEGG: ssn:SSON_1407 hypothetical protein; hypothetical protein complement(2003955..2004665) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158971 YP_003036127.1 CDS ECBD_1895 NC_012947.1 2004832 2005650 R removes the damaged DNA at cytosines and guanines by cleaving on the 3' side of the AP site by a beta-elimination reaction; exonuclease III complement(2004832..2005650) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158972 YP_003036128.1 CDS ECBD_1896 NC_012947.1 2005651 2005821 R KEGG: sbc:SbBS512_E1996 hypothetical protein; hypothetical protein complement(2005651..2005821) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158973 YP_003036129.1 CDS ECBD_1897 NC_012947.1 2006084 2007304 D catalyzes the transamination of 2-N-succinylornithine and alpha-ketoglutarate into 2-N-succinylglutamate semialdehyde and glutamate; also functions as the catabolic acetylornithine aminotransferase catalyzing the formation of 2-N-acetylglutamate semialdehyde and glutamate from 2-N-acetylornithine and alpha-ketoglutarate; bifunctional succinylornithine transaminase/acetylornithine transaminase 2006084..2007304 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158974 YP_003036130.1 CDS ECBD_1898 NC_012947.1 2007301 2008335 D TIGRFAM: arginine N-succinyltransferase; arginine/ornithine succinyltransferase subunit; PFAM: arginine N-succinyltransferase subunit beta; arginine succinyltransferase 2007301..2008335 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158975 YP_003036131.1 CDS astD NC_012947.1 2008332 2009810 D a high throughput screen identified this enzyme as an aldehyde dehydrogenase with broad substrate specificity; converts succinylglutamate semialdehyde and NAD to succinylglutamate and NADH; succinylglutamic semialdehyde dehydrogenase 2008332..2009810 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158976 YP_003036132.1 CDS ECBD_1900 NC_012947.1 2009807 2011150 D catalyzes the hydrolysis of 2-N-succinylarginine into 2-N-succinylornithine, ammonia and carbon dioxide in arginine degradation; succinylarginine dihydrolase 2009807..2011150 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158977 YP_003036133.1 CDS ECBD_1901 NC_012947.1 2011143 2012111 D catalyzes the formation of succinate and glutamate from N(2)-succinylglutamate in arginine catabolism; succinylglutamate desuccinylase 2011143..2012111 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158978 YP_003036134.1 CDS ECBD_1902 NC_012947.1 2012441 2012926 D periplasmic protein induced by stress response via Cpx and BaeSR system; similar to CpxP; periplasmic protein 2012441..2012926 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158979 YP_003036135.1 CDS ECBD_1903 NC_012947.1 2013129 2013704 D PFAM: protein of unknown function DUF886; KEGG: sfv:SFV_1478 hypothetical protein; hypothetical protein 2013129..2013704 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158980 YP_003036136.1 CDS ECBD_1904 NC_012947.1 2013664 2014551 R 3' incision activity; acts with UvrC; nucleotide excision repair endonuclease complement(2013664..2014551) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158981 YP_003036137.1 CDS nadE NC_012947.1 2014781 2015608 R catalyzes the formation of nicotinamide adenine dinucleotide (NAD) from nicotinic acid adenine dinucleotide (NAAD) using either ammonia or glutamine as the amide donor and ATP; ammonia-utilizing enzymes include the ones from Bacillus and Escherichia coli while glutamine-utilizing enzymes include the Mycobacterial one; forms homodimers; NAD synthetase complement(2014781..2015608) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158982 YP_003036138.1 CDS ECBD_1906 NC_012947.1 2015810 2016148 D osmotically-inducible lipoprotein E; activator of ntr-like gene protein; DNA-binding transcriptional activator OsmE 2015810..2016148 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158983 YP_003036139.1 CDS ECBD_1907 NC_012947.1 2016447 2016767 D catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane; involved in N,N'-diacetylchitobiose transport; protein IIA transfers a phosphoryl group to IIB which then transfers the phosphoryl group to the sugar; IIC forms the translocation channel for the sugar uptake; PTS system N,N'-diacetylchitobiose-specific transporter subunit IIB 2016447..2016767 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158984 YP_003036140.1 CDS ECBD_1908 NC_012947.1 2016852 2018210 D catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane; involved in N,N'-diacetylchitobiose transport; protein IIA transfers a phosphoryl group to IIB which then transfers the phosphoryl group to the sugar; IIC forms the translocation channel for the sugar uptake; PTS system N,N'-diacetylchitobiose-specific transporter subunit IIC 2016852..2018210 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158985 YP_003036141.1 CDS ECBD_1909 NC_012947.1 2018261 2018611 D catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane; involved N,N'-diacetylchitobiose transport; protein IIA transfers a phosphoryl group to IIB which then transfers the phosphoryl group to the sugar; IIC forms the translocation channel for the sugar uptake; PTS system N,N'-diacetylchitobiose-specific transporter subunit IIA 2018261..2018611 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158986 YP_003036142.1 CDS ECBD_1910 NC_012947.1 2018622 2019461 D represses the celABCDF-ydjC operon involved in carbon uptake; DNA-binding transcriptional regulator ChbR 2018622..2019461 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158987 YP_003036143.1 CDS ECBD_1911 NC_012947.1 2019566 2020918 D PFAM: glycoside hydrolase family 4; KEGG: elf:LF82_0293 6-phospho-beta-glucosidase; glycoside hydrolase family 4 2019566..2020918 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158988 YP_003036144.1 CDS ECBD_1912 NC_012947.1 2020931 2021680 D PFAM: YdjC family protein; KEGG: ssn:SSON_1425 hypothetical protein; hypothetical protein 2020931..2021680 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158989 YP_003036145.1 CDS katE NC_012947.1 2021938 2024199 R catalase HPII; monofunctional catalase that decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide; hydroperoxidase II complement(2021938..2024199) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157181 YP_003036146.1 CDS ECBD_1914 NC_012947.1 2024382 2024645 D KEGG: ssn:SSON_1427 cell division modulator; cell division modulator 2024382..2024645 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157182 YP_003036147.1 CDS ECBD_1915 NC_012947.1 2024934 2025716 D KEGG: sfl:SF1498 hypothetical protein; hypothetical protein 2024934..2025716 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157183 YP_003036148.1 CDS ECBD_1916 NC_012947.1 2025741 2027132 R PFAM: sodium:dicarboxylate symporter; sodium:dicarboxylate symporter complement(2025741..2027132) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157184 YP_003036149.1 CDS ECBD_1917 NC_012947.1 2027265 2027855 R PFAM: membrane-bound metal-dependent hydrolase; KEGG: sdy:SDY_1821 hypothetical protein; hypothetical protein complement(2027265..2027855) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157185 YP_003036150.1 CDS ECBD_1918 NC_012947.1 2028018 2028686 R YniC; catalyzes the dephosphorylation of 2-deoxyglucose 6-phosphate, mannose 6-phosphate and p-nitrophenyl phosphate; 2-deoxyglucose-6-phosphatase complement(2028018..2028686) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157186 YP_003036151.1 CDS ECBD_1919 NC_012947.1 2028833 2029369 D KEGG: ssn:SSON_1431 hypothetical protein; hypothetical protein 2028833..2029369 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157187 YP_003036152.1 CDS ECBD_1920 NC_012947.1 2029410 2030270 R PFAM: fructosamine kinase; aminoglycoside phosphotransferase; KEGG: sdy:SDY_1818 hypothetical protein; fructosamine kinase complement(2029410..2030270) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157188 YP_003036153.1 CDS ECBD_1921 NC_012947.1 2030376 2030666 R KEGG: ssn:SSON_1433 hypothetical protein; hypothetical protein complement(2030376..2030666) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157189 YP_003036154.1 CDS ECBD_1922 NC_012947.1 2030767 2031696 R TIGRFAM: 1-phosphofructokinase; PFAM: PfkB domain protein; KEGG: ssn:SSON_1434 6-phosphofructokinase 2; 6-phosphofructokinase 2 complement(2030767..2031696) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157190 YP_003036155.1 CDS ECBD_1923 NC_012947.1 2031983 2032741 D PFAM: protein of unknown function DUF481; KEGG: ssn:SSON_1435 hypothetical protein; hypothetical protein 2031983..2032741 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157191 YP_003036156.1 CDS ECBD_1924 NC_012947.1 2032794 2032901 R KEGG: ssn:SSON_1436 hypothetical protein; hypothetical protein complement(2032794..2032901) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157192 YP_003036157.1 CDS ECBD_1926 NC_012947.1 2035494 2037422 D catalyzes the formation of threonyl-tRNA(Thr) from threonine and tRNA(Thr); catalyzes a two-step reaction, first charging a threonine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; threonyl-tRNA synthetase 2035494..2037422 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157194 YP_003036158.1 CDS infC NC_012947.1 2037426 2037968 D IF-3 has several functions that are required and promote translation initiation including; preventing association of 70S by binding to 30S; monitoring codon-anticodon interactions by promoting disassociation of fMet-tRNA(fMet) from initiation complexes formed on leaderless mRNAs or incorrectly bound noninitiatior tRNAs and complexes with noncanonical start sites; stimulates codon-anticodon interactions at P-site; involved in moving mRNA to the P-site; and in recycling subunits; translation initiation factor IF-3 2037426..2037968 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157195 YP_003036159.1 CDS rpmI NC_012947.1 2038065 2038262 D TIGRFAM: ribosomal protein L35; PFAM: ribosomal protein L35; KEGG: sea:SeAg_B1838 50S ribosomal protein L35; 50S ribosomal protein L35 2038065..2038262 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157196 YP_003036160.1 CDS rplT NC_012947.1 2038315 2038671 D binds directly to 23S ribosomal RNA prior to in vitro assembly of the 50S ribosomal subunit; 50S ribosomal protein L20 2038315..2038671 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157197 YP_003036161.1 CDS ECBD_1930 NC_012947.1 2038913 2039008 D hypothetical protein 2038913..2039008 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157198 YP_003036162.1 CDS pheS NC_012947.1 2039122 2040105 D catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a heterotetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 1 subfamily; phenylalanyl-tRNA synthetase subunit alpha 2039122..2040105 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157199 YP_003036163.1 CDS pheT NC_012947.1 2040120 2042507 D catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a tetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 2 subfamily; phenylalanyl-tRNA synthetase subunit beta 2040120..2042507 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157200 YP_003036164.1 CDS ihfA NC_012947.1 2042512 2042811 D This protein is one of the two subunits of integration host factor, a specific DNA-binding protein that functions in genetic recombination as well as in transcriptional and translational control; integration host factor subunit alpha 2042512..2042811 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157201 YP_003036165.1 CDS ECBD_1934 NC_012947.1 2042912 2043892 D with BtuD and BtuF transports vitamin B12 into the cell; vtamin B12-transporter permease 2042912..2043892 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157202 YP_003036166.1 CDS btuE NC_012947.1 2043955 2044506 D similar to glutathione peroxidase; member of the btuCED operon which is required for vitamin B12 transport across the inner membrane; glutathione peroxidase 2043955..2044506 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157203 YP_003036167.1 CDS ECBD_1936 NC_012947.1 2044506 2045255 D ATP-binding protein that acts with the transmembrane protein BtuC and the solute binding protein BtuF to transport vitamin B12 into the cell; vitamin B12-transporter ATPase 2044506..2045255 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157204 YP_003036168.1 CDS ECBD_1937 NC_012947.1 2045333 2045797 D PFAM: NLP/P60 protein; KEGG: elf:LF82_1500 lipoprotein NlpC; NLP/P60 protein 2045333..2045797 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157205 YP_003036169.1 CDS ECBD_1939 NC_012947.1 2046819 2048255 D PFAM: protein of unknown function UPF0061; KEGG: sfv:SFV_1518 hypothetical protein; hypothetical protein 2046819..2048255 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157207 YP_003036170.1 CDS ECBD_1940 NC_012947.1 2048259 2048450 R KEGG: sdy:SDY_1799 hypothetical protein; hypothetical protein complement(2048259..2048450) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157208 YP_003036171.1 CDS ECBD_1941 NC_012947.1 2048582 2049628 R tryptophan sensitive; catalyzes the formation of 3-deoxy-D-arabino-hept-2-ulosonate 7 phosphate from phosphoenolpyruvate and D-erythrose 4-phosphate; phospho-2-dehydro-3-deoxyheptonate aldolase complement(2048582..2049628) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157209 YP_003036172.1 CDS ECBD_1942 NC_012947.1 2049785 2050618 R PFAM: protein of unknown function DUF299; KEGG: sdy:SDY_1797 hypothetical protein; hypothetical protein complement(2049785..2050618) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157210 YP_003036173.1 CDS ECBD_1943 NC_012947.1 2050730 2050897 D hypothetical protein 2050730..2050897 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157211 YP_003036174.1 CDS ECBD_1944 NC_012947.1 2050951 2053329 D catalyzes the formation of phosphoenolpyruvate from pyruvate; phosphoenolpyruvate synthase 2050951..2053329 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157212 YP_003036175.1 CDS ECBD_1945 NC_012947.1 2053386 2055032 R acts in anaerobic beta-oxidation of fatty acids; ligase/synthase involved in anaerobic growth on fatty acids; short chain acyl-CoA synthetase complement(2053386..2055032) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157213 YP_003036176.1 CDS ECBD_1946 NC_012947.1 2055089 2055382 R KEGG: sbc:SbBS512_E1903 iron-sulfur cluster-binding protein; iron-sulfur cluster-binding protein complement(2055089..2055382) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157214 YP_003036177.1 CDS ECBD_1947 NC_012947.1 2055379 2056668 R PFAM: FAD dependent oxidoreductase; monooxygenase FAD-binding; HI0933 family protein; KEGG: sfv:SFV_1525 hypothetical protein; hypothetical protein complement(2055379..2056668) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157215 YP_003036178.1 CDS ECBD_1948 NC_012947.1 2056724 2057662 R May play a role in a redox process; electron transfer flavoprotein subunit YdiR complement(2056724..2057662) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157216 YP_003036179.1 CDS ECBD_1949 NC_012947.1 2057682 2058446 R YdiO, YdiQ, YdiR, YdiS, and YdiT may play a role in electron transport between the anaerobic fatty acid oxidation pathway and the respiratory chain; electron transfer flavoprotein YdiQ complement(2057682..2058446) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157217 YP_003036180.1 CDS ECBD_1950 NC_012947.1 2058762 2059673 D PFAM: cupin; helix-turn-helix- domain containing protein AraC type; SMART: helix-turn-helix- domain containing protein AraC type; KEGG: sbc:SbBS512_E1899 AraC family transcriptional regulator; AraC family transcriptional regulator 2058762..2059673 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157218 YP_003036181.1 CDS ECBD_1951 NC_012947.1 2059716 2060867 R may play a role in electron transport between the anaerobic fatty acid oxidation pathway and the respiratory chain; acyl-CoA dehydrogenase complement(2059716..2060867) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157219 YP_003036182.1 CDS ECBD_1952 NC_012947.1 2060881 2062476 R PFAM: coenzyme A transferase; KEGG: elf:LF82_2872 uncharacterized protein YdiF; coenzyme A transferase complement(2060881..2062476) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157220 YP_003036183.1 CDS aroD NC_012947.1 2062621 2063379 R catalyzes the dehydration of 3-dehydroquinate to form 3-dehydroshikimate in aromatic amino acid biosynthesis; 3-dehydroquinate dehydratase complement(2062621..2063379) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157221 YP_003036184.1 CDS ECBD_1954 NC_012947.1 2063410 2064276 R YdiB; quinate/shikimate dehydrogenase from Escherichia coli uses both NAD and NAD(P) to convert quinate and shikimate to 3-dehydroquinate and 3-dehydroshikimate; quinate/shikimate dehydrogenase complement(2063410..2064276) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157222 YP_003036185.1 CDS ECBD_1955 NC_012947.1 2064288 2065553 R PFAM: major facilitator superfamily MFS_1; major facilitator superfamily MFS_1 complement(2064288..2065553) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157223 YP_003036186.1 CDS ECBD_1957 NC_012947.1 2067093 2067449 R PFAM: Domain of unknown function DUF1870; KEGG: ssn:SSON_1466 hypothetical protein; hypothetical protein complement(2067093..2067449) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157225 YP_003036187.1 CDS ECBD_1958 NC_012947.1 2067878 2068990 R YdiK; inner membrane protein; ydiK promoter presents a PurR sequence, suggesting that its expression is purine-regulated; unknown function; inner membrane protein complement(2067878..2068990) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157226 YP_003036188.1 CDS ECBD_1959 NC_012947.1 2069379 2072435 D PFAM: FAD linked oxidase; KEGG: ssn:SSON_1469 oxidase; FAD linked oxidase 2069379..2072435 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157227 YP_003036189.1 CDS ECBD_1960 NC_012947.1 2072432 2072842 D PFAM: thioesterase superfamily protein; KEGG: ssn:SSON_1470 hypothetical protein; hypothetical protein 2072432..2072842 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157228 YP_003036190.1 CDS ECBD_1961 NC_012947.1 2072861 2073130 D KEGG: sfv:SFV_1708 hypothetical protein; hypothetical protein 2072861..2073130 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157229 YP_003036191.1 CDS ECBD_1962 NC_012947.1 2073678 2074688 D PFAM: SufBD protein; KEGG: sbo:SBO_1447 cysteine desulfurase activator complex subunit SufB; SufBD protein 2073678..2074688 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157230 YP_003036192.1 CDS sufC NC_012947.1 2074698 2075444 D part of SUF system involved in inserting iron-sulfur clusters into proteins; in Escherichia coli this protein forms a complex with SufBD; the SufBCD complex stimulates the cysteine desulfurase SufS in conjunction with SufE; cysteine desulfurase ATPase component 2074698..2075444 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157231 YP_003036193.1 CDS ECBD_1964 NC_012947.1 2075419 2076690 D with SufBC activates cysteine desulfurase SufS; cysteine desulfurase activator complex subunit SufD 2075419..2076690 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157232 YP_003036194.1 CDS ECBD_1965 NC_012947.1 2076687 2077907 D catalyzes the formation of L-alanine and selenide or sulfanylcysteine from selenocysteine or cysteine; bifunctional cysteine desulfurase/selenocysteine lyase 2076687..2077907 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157233 YP_003036195.1 CDS ECBD_1966 NC_012947.1 2077920 2078336 D Acts with SufS to catalyze the formation of L-alanine from L-cysteine; cysteine desufuration protein SufE 2077920..2078336 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157234 YP_003036196.1 CDS ECBD_1967 NC_012947.1 2078485 2079489 D PFAM: ErfK/YbiS/YcfS/YnhG family protein; peptidoglycan-binding LysM; SMART: peptidoglycan-binding LysM; KEGG: sfv:SFV_1701 hypothetical protein; ErfK/YbiS/YcfS/YnhG family protein 2078485..2079489 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157235 YP_003036197.1 CDS ECBD_1968 NC_012947.1 2079553 2079789 R PFAM: LPP repeat-containing protein; KEGG: ssn:SSON_1479 murein lipoprotein; LPP repeat-containing protein complement(2079553..2079789) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157236 YP_003036198.1 CDS ECBD_1969 NC_012947.1 2080100 2081512 R catalyzes the formation of phosphoenolpyruvate from pyruvate; pyruvate kinase complement(2080100..2081512) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157237 YP_003036199.1 CDS ECBD_1970 NC_012947.1 2082069 2082278 D KEGG: ssn:SSON_1481 hypothetical protein; hypothetical protein 2082069..2082278 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157238 YP_003036200.1 CDS ECBD_1971 NC_012947.1 2082733 2083359 D PFAM: 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: sbo:SBO_1458 hypothetical protein; hypothetical protein 2082733..2083359 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157239 YP_003036201.1 CDS ECBD_1972 NC_012947.1 2083380 2085482 D PFAM: aldehyde ferredoxin oxidoreductase ; aldehyde ferredoxin oxidoreductase; SMART: aldehyde ferredoxin oxidoreductase; oxidoreductase 2083380..2085482 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157240 YP_003036202.1 CDS ECBD_1973 NC_012947.1 2085495 2086133 D KEGG: sbo:SBO_1460 hypothetical protein; hypothetical protein 2085495..2086133 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157241 YP_003036203.1 CDS ECBD_1974 NC_012947.1 2086197 2086865 D PFAM: 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: sfv:SFV_1693 oxidoreductase, Fe-S subunit; 4Fe-4S ferredoxin iron-sulfur binding domain protein 2086197..2086865 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157242 YP_003036204.1 CDS ECBD_1975 NC_012947.1 2086862 2087647 D PFAM: cytochrome B561; KEGG: sbc:SbBS512_E1870 nickel-dependent hydrogenase, b-type cytochrome subunit; cytochrome B561 2086862..2087647 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157243 YP_003036205.1 CDS ECBD_1976 NC_012947.1 2087699 2088463 D KEGG: sdy:SDY_1897 hypothetical protein; hypothetical protein 2087699..2088463 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157244 YP_003036206.1 CDS ECBD_1977 NC_012947.1 2088475 2090079 R KEGG: ssn:SSON_1488 hypothetical protein; hypothetical protein complement(2088475..2090079) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157245 YP_003036207.1 CDS ECBD_1978 NC_012947.1 2090205 2090510 R PFAM: mono-oxygenase ydhR; KEGG: sbc:SbBS512_E1865 YdhR protein; hypothetical protein complement(2090205..2090510) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157246 YP_003036208.1 CDS ECBD_1979 NC_012947.1 2091083 2092339 D KEGG: ssn:SSON_1492 hypothetical protein; hypothetical protein 2091083..2092339 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157249 YP_003036209.1 CDS ECBD_1980 NC_012947.1 2092380 2093753 R NorM; MdtK; functions as a Na(+)/drug antiporter; inactivation in Vibrio cholerae results in susceptibility to fluoroquinolones; multidrug efflux protein complement(2092380..2093753) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157250 YP_003036210.1 CDS ECBD_1981 NC_012947.1 2093968 2094609 D catalyzes the formation of riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine; riboflavin synthase subunit alpha 2093968..2094609 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157251 YP_003036211.1 CDS ECBD_1982 NC_012947.1 2094649 2095797 R catalyzes the transfer of a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge; cyclopropane fatty acyl phospholipid synthase complement(2094649..2095797) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157252 YP_003036212.1 CDS ECBD_1983 NC_012947.1 2096088 2097299 R uncharacterized member of the major facilitator superfamily (MFS); inner membrane transporter YdhC complement(2096088..2097299) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157253 YP_003036213.1 CDS ECBD_1984 NC_012947.1 2097412 2098344 D PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: sdy:SDY_1885 DNA-binding transcriptional regulator; DNA-binding transcriptional regulator 2097412..2098344 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157254 YP_003036214.1 CDS ECBD_1985 NC_012947.1 2098341 2099366 R binds to the purF operator and coregulates other genes for de novo purine nucleotide synthesis; is involved in regulation of purB, purC, purEK, purHD, purL, purMN and guaBA expression; binds hypoxanthine and guanine as inducers; DNA-binding transcriptional repressor PurR complement(2098341..2099366) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157255 YP_003036215.1 CDS ECBD_1986 NC_012947.1 2099920 2101089 D PFAM: major facilitator superfamily MFS_1; KEGG: sdy:SDY_1883 transporter; major facilitator superfamily MFS_1 2099920..2101089 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157256 YP_003036216.1 CDS ECBD_1987 NC_012947.1 2101324 2101905 R SodB; iron binding; present under aerobic and anaerobic conditions; destroys free radicals; superoxide dismutase complement(2101324..2101905) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157257 YP_003036217.1 CDS ECBD_1988 NC_012947.1 2102033 2102848 R PFAM: NLP/P60 protein; KEGG: sbc:SbBS512_E1852 NlpC/P60 family protein; NLP/P60 protein complement(2102033..2102848) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157258 YP_003036218.1 CDS ECBD_1989 NC_012947.1 2103182 2103529 D TIGRFAM: glutaredoxin-like protein; PFAM: glutaredoxin; KEGG: sdy:SDY_1880 hypothetical protein; hypothetical protein 2103182..2103529 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157259 YP_003036219.1 CDS ECBD_1990 NC_012947.1 2103580 2108196 R similar to the DNA and RNA helicase superfamily II and eukaryotic DEAD family of helicases; longest known protein in E. coli; ATP-dependent helicase Lhr complement(2103580..2108196) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157260 YP_003036220.1 CDS ECBD_1991 NC_012947.1 2108289 2108936 R Responsible for the end-turnover of tRNA: specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A); ribonuclease T complement(2108289..2108936) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157261 YP_003036221.1 CDS ECBD_1992 NC_012947.1 2109039 2109446 R Ni-dependent; catalyzes the formation of S-lactoylglutathione from methylglyoxal and glutathione; glyoxalase I complement(2109039..2109446) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157262 YP_003036222.1 CDS ECBD_1993 NC_012947.1 2109527 2110624 R FMN-linked; catalyzes the formation of N-ethylsuccinimide from N-ethylmaleimide; N-ethylmaleimide reductase complement(2109527..2110624) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158493 YP_003036223.1 CDS ECBD_1994 NC_012947.1 2110661 2111260 R PFAM: regulatory protein TetR; KEGG: sbc:SbBS512_E1841 TetR family transcriptional regulator; TetR family transcriptional regulator complement(2110661..2111260) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157100 YP_003036224.1 CDS ECBD_1995 NC_012947.1 2111363 2111602 D PFAM: protein of unknown function DUF1289; KEGG: sdy:SDY_1873 hypothetical protein; hypothetical protein 2111363..2111602 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157101 YP_003036225.1 CDS ECBD_1996 NC_012947.1 2111651 2112547 D PFAM: aldo/keto reductase; KEGG: efe:EFER_1396 oxidoreductase; aldo/keto reductase 2111651..2112547 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157102 YP_003036226.1 CDS ECBD_1997 NC_012947.1 2112628 2113149 D SodC; copper and zinc binding; converts superoxide radicals to hydrogen peroxide and water; superoxide dismutase 2112628..2113149 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157103 YP_003036227.1 CDS ECBD_1998 NC_012947.1 2113150 2115162 R PFAM: fusaric acid resistance protein conserved region; fusaric acid resistance protein conserved region complement(2113150..2115162) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157104 YP_003036228.1 CDS ECBD_1999 NC_012947.1 2115162 2116019 R PFAM: secretion protein HlyD; KEGG: sbc:SbBS512_E1836 auxiliary transporter, membrane fusion protein (MFP) family; secretion protein HlyD complement(2115162..2116019) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157105 YP_003036229.1 CDS ECBD_2000 NC_012947.1 2116022 2116258 R PFAM: protein of unknown function DUF1656; KEGG: sfl:SF1670 hypothetical protein; hypothetical protein complement(2116022..2116258) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157106 YP_003036230.1 CDS ECBD_2001 NC_012947.1 2116459 2116893 D transcription regulator that can both activate or repress expression; transcriptional regulator SlyA 2116459..2116893 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157107 YP_003036231.1 CDS ECBD_2002 NC_012947.1 2116940 2117407 R PFAM: 17 kDa surface antigen; KEGG: sfl:SF1668 outer membrane protein; 17 kDa surface antigen complement(2116940..2117407) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157108 YP_003036232.1 CDS anmK NC_012947.1 2117681 2118790 D catalyzes hydrolysis of 1,6-anhydro bond of anyhydro-N-acetylmuramic acid (anhMurNAc) and phosphorylates anhMurNAc to produce N-acetyl-muramate-6-phosphate; involved in murein recycling; anhydro-N-acetylmuramic acid kinase 2117681..2118790 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157109 YP_003036233.1 CDS ECBD_2004 NC_012947.1 2118894 2119217 D MliC; membrane-bound lysozyme inhibitor of c-type lysozyme; lysozyme inhibitor 2118894..2119217 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157110 YP_003036234.1 CDS ECBD_2005 NC_012947.1 2119276 2119932 D catalyzes the formation of pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate; pyridoxamine 5'-phosphate oxidase 2119276..2119932 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157111 YP_003036235.1 CDS ECBD_2006 NC_012947.1 2120061 2121335 D catalyzes the formation of tyrosyl-tRNA(Tyr) from tyrosine and tRNA(Tyr); tyrosyl-tRNA synthetase 2120061..2121335 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157112 YP_003036236.1 CDS ECBD_2007 NC_012947.1 2121397 2122257 D catalyzes the formation of pyridoxal 5'-phosphate from pyridoxal; pyridoxamine kinase 2121397..2122257 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157113 YP_003036237.1 CDS ECBD_2008 NC_012947.1 2122301 2122906 R PFAM: glutathione S-transferase domain; KEGG: sdy:SDY_1858 glutathionine S-transferase; glutathionine S-transferase complement(2122301..2122906) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157114 YP_003036238.1 CDS tppB NC_012947.1 2123012 2124514 R mutations in this gene confer resistance to the toxic peptide alafosfalin in Salmonella typhimurium; member of proton-dependent oligopeptide transport (POT) system family; in Escherichia coli this gene is regulated by OmpR although not via osmoregulation; tripeptide transporter permease complement(2123012..2124514) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157115 YP_003036239.1 CDS ECBD_2010 NC_012947.1 2124797 2124997 R KEGG: sbc:SbBS512_E1823 hypothetical protein; hypothetical protein complement(2124797..2124997) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157116 YP_003036240.1 CDS ECBD_2011 NC_012947.1 2125125 2125760 R DNA-(apurinic or apyrimidinic site) lyase; has apurinic or apyrimidinic endonuclease activity and DNA N-glycosylase activity; removed damaged DNA at cytosines, thymines and guanines; endonuclease III complement(2125125..2125760) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157117 YP_003036241.1 CDS ECBD_2012 NC_012947.1 2125760 2126455 R in Excherichia coli RsxABCDEG reduces SoxR which turns off induction of SoxS transcription factor in the absence of oxidizing agents; similar to the rnfABCDGE operon in Rhodobacter capsulatus involved in transferring electrons to nitrogenase; electron transport complex RsxE subunit complement(2125760..2126455) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157118 YP_003036242.1 CDS ECBD_2013 NC_012947.1 2126459 2127079 R part of membrane-bound complex hought to be involved in electron transport to nitrogen; electron transport complex protein RnfG complement(2126459..2127079) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157119 YP_003036243.1 CDS rnfD NC_012947.1 2127083 2128141 R RnfD; RsxD; required for nitrogen fixation in Rhodobacter capsulatus; part of a membrane-bound complex thought to be involved in electron transport to nitrogenase; in Escherichia coli this gene is part of a cluster controlling SoxR-mediated induction of the SoxS transcription factor in the absence of oxidizing agents; electron transport complex protein RnfD complement(2127083..2128141) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157120 YP_003036244.1 CDS ECBD_2015 NC_012947.1 2128142 2130268 R part of membrane-bound complex thought to be involved in electron transport to nitrogen; electron transport complex protein RnfC complement(2128142..2130268) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157121 YP_003036245.1 CDS ECBD_2016 NC_012947.1 2130261 2130839 R part of membrane-bound complex thought to be involved in electron transport to nitrogen; electron transport complex protein RnfB complement(2130261..2130839) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157122 YP_003036246.1 CDS ECBD_2017 NC_012947.1 2130839 2131420 R TIGRFAM: electron transport complex, RnfABCDGE type, A subunit; PFAM: RnfA-Nqr electron transport subunit; KEGG: sbc:SbBS512_E1816 electron transport complex, RnfABCDGE type, subunit alpha; Na(+)-translocating NADH-quinone reductase subunit E complement(2130839..2131420) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157123 YP_003036247.1 CDS ECBD_2018 NC_012947.1 2131497 2131937 R KEGG: ssn:SSON_1532 hypothetical protein; hypothetical protein complement(2131497..2131937) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157124 YP_003036248.1 CDS ECBD_2019 NC_012947.1 2132023 2132238 R YdgT; in E. coli, when complexed with H-NS or StpA binds a 26 base-pair DNA sequence of oriC; seems to play a role in optimizing the activity of oriC; non-essential; Hha protein paralog; in an hha mutant background, cnu is overexpressed and can compensate hha-induced phenotypes; oriC-binding nucleoid-associated protein complement(2132023..2132238) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157125 YP_003036249.1 CDS ECBD_2020 NC_012947.1 2132511 2132711 R KEGG: ses:SARI_01519 hypothetical protein; hypothetical protein complement(2132511..2132711) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157126 YP_003036250.1 CDS ECBD_2021 NC_012947.1 2132879 2133919 D PFAM: oxidoreductase domain protein; semialdehyde dehydrogenase NAD - binding; oxidoreductase domain; KEGG: sbo:SBO_1510 oxidoreductase; oxidoreductase 2132879..2133919 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157127 YP_003036251.1 CDS ECBD_2022 NC_012947.1 2133953 2134954 R catalyzes the formation of inosine from adenosine; adenosine deaminase complement(2133953..2134954) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157128 YP_003036252.1 CDS ECBD_2023 NC_012947.1 2135058 2136230 R PFAM: aminotransferase class I and II; KEGG: elf:LF82_1268 protein MalY; aminotransferase class I and II complement(2135058..2136230) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157129 YP_003036253.1 CDS ECBD_2024 NC_012947.1 2136240 2137832 R phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS); catalyzes the phosphorylation of maltose and glucose concomitant with their translocation across the cell membrane; component IIB catalyzes the phosphorylation of the sugar molecule; IIC forms the translocation channel and contains the substrate specific binding site; bifunctional PTS system maltose and glucose-specific transporter subunits IICB complement(2136240..2137832) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157130 YP_003036254.1 CDS ECBD_2025 NC_012947.1 2138007 2139035 D regulates malXY which are involved in maltose-glucose transport; DNA-binding transcriptional repressor MalI 2138007..2139035 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157131 YP_003036255.1 CDS ECBD_2026 NC_012947.1 2139146 2139913 D Acts on the hydroxyl group at position 7 of the steroid frame; 7-alpha-hydroxysteroid dehydrogenase 2139146..2139913 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157132 YP_003036256.1 CDS ECBD_2027 NC_012947.1 2140146 2140724 D PFAM: regulatory protein TetR; KEGG: sdy:SDY_1837 repressor for uid operon; TetR family transcriptional regulator 2140146..2140724 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157133 YP_003036257.1 CDS ECBD_2028 NC_012947.1 2141115 2142926 D PFAM: glycoside hydrolase family 2 TIM barrel; glycoside hydrolase family 2 sugar binding; glycoside hydrolase family 2 immunoglobulin domain protein beta-sandwich; KEGG: ssn:SSON_1543 beta-D-glucuronidase; beta-D-glucuronidase 2141115..2142926 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157134 YP_003036258.1 CDS ECBD_2029 NC_012947.1 2142923 2144296 D TIGRFAM: sugar (glycoside-Pentoside-Hexuronide) transporter; PFAM: major facilitator superfamily MFS_1; KEGG: ssn:SSON_1544 glucuronide transporter; glucuronide transporter 2142923..2144296 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157135 YP_003036259.1 CDS ECBD_2030 NC_012947.1 2144335 2145600 D PFAM: outer membrane porin; KEGG: elf:LF82_2370 membrane-associated protein UidC; outer membrane porin protein 2144335..2145600 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157136 YP_003036260.1 CDS ECBD_2031 NC_012947.1 2145661 2145756 R hypothetical protein complement(2145661..2145756) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157137 YP_003036261.1 CDS ECBD_2032 NC_012947.1 2145826 2147334 R PFAM: protein of unknown function DUF945; KEGG: sbc:SbBS512_E1800 hypothetical protein; hypothetical protein complement(2145826..2147334) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157138 YP_003036262.1 CDS ECBD_2033 NC_012947.1 2147435 2148610 R KEGG: sfv:SFV_1629 mannose-6-phosphate isomerase; TIGRFAM: mannose-6-phosphate isomerase, class I; PFAM: mannose-6-phosphate isomerase type I; mannose-6-phosphate isomerase, class I complement(2147435..2148610) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157139 YP_003036263.1 CDS ECBD_2034 NC_012947.1 2148809 2150455 D TIGRFAM: hydro-lyase, Fe-S type, tartrate/fumarate subfamily subunit beta; hydro-lyase, Fe-S type, tartrate/fumarate subfamily, subunit alpha; PFAM: Fe-S type hydro-lyase tartrate/fumarate alpha region; Fe-S type hydro-lyase tartrate/fumarate beta region; KEGG: elf:LF82_0776 fumarate hydratase class I, aerobic; hydro-lyase, Fe-S type, tartrate/fumarate subfamily, subunit beta 2148809..2150455 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157140 YP_003036264.1 CDS fumC NC_012947.1 2150598 2152001 D class II family (does not require metal); tetrameric enzyme; fumarase C; reversibly converts (S)-malate to fumarate and water; functions in the TCA cycle; fumarate hydratase 2150598..2152001 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157141 YP_003036265.1 CDS ECBD_2036 NC_012947.1 2151998 2152927 R binds to DNA replication terminator sequences to prevent passage of replication forks; DNA replication terminus site-binding protein complement(2151998..2152927) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157142 YP_003036266.1 CDS ECBD_2037 NC_012947.1 2153003 2154304 R PFAM: ATP-binding region ATPase domain protein; histidine kinase HAMP region domain protein; histidine kinase A domain protein; SMART: ATP-binding region ATPase domain protein; histidine kinase A domain protein; histidine kinase HAMP region domain protein; KEGG: sdy:SDY_1825 sensor protein RstB; sensor protein RstB complement(2153003..2154304) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157143 YP_003036267.1 CDS ECBD_2038 NC_012947.1 2154308 2155027 R response regulator in two-component regulatory system with RstB; DNA-binding transcriptional regulator RstA complement(2154308..2155027) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157144 YP_003036268.1 CDS ECBD_2039 NC_012947.1 2155156 2155491 D PFAM: GlpM family protein; KEGG: sdy:SDY_1546 hypothetical protein; GlpM family protein 2155156..2155491 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157145 YP_003036269.1 CDS ECBD_2040 NC_012947.1 2155488 2156210 R PFAM: short-chain dehydrogenase/reductase SDR; KEGG: sdy:SDY_1547 short chain dehydrogenase; short chain dehydrogenase complement(2155488..2156210) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157146 YP_003036270.1 CDS ECBD_2041 NC_012947.1 2156247 2157629 R TIGRFAM: arginine/ornithine antiporter; PFAM: amino acid permease-associated region; KEGG: sfv:SFV_1620 arginine/ornithine antiporter; arginine/ornithine antiporter complement(2156247..2157629) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157147 YP_003036271.1 CDS ECBD_2042 NC_012947.1 2157815 2158759 R PFAM: protein of unknown function DUF1471; KEGG: elf:LF82_2844 protein YdgH; hypothetical protein complement(2157815..2158759) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157148 YP_003036272.1 CDS pntA NC_012947.1 2159283 2160815 D forms a tetramer composed of 2 subunit alphas and 2 beta subunits in the inner membrane; involved in catalyzing transfer of hydride ion equivalents between NAD and NADP; stereospecific (AB-specific); functions as a proton pump by translocating protons from cytoplasm to periplasm; NAD(P) transhydrogenase subunit alpha 2159283..2160815 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157149 YP_003036273.1 CDS pntB NC_012947.1 2160826 2162214 D catalyzes reversible transfer of hydride ion equivalent between NAD and NADP; membrane-bound proton pump that translocates protons from cytosolic to periplasmic side of the inner membrane; forms a tetramer composed of two alpha and 2 subunit betas; AB-stereospecific enzyme; pyridine nucleotide transhydrogenase 2160826..2162214 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157150 YP_003036274.1 CDS tqsA NC_012947.1 2162239 2163273 R transport of quorum-sensing signal; mutations in this gene alters the transport of the quorum-sensing signal autoinducer 2 (AI-2) which affects expression of a large number of genes; transporter complement(2162239..2163273) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157151 YP_003036275.1 CDS ECBD_2046 NC_012947.1 2163685 2164050 D with MdtI is involved in resistance to deoxycholate , nalidixic acid, fosfomycin, and SDS; multidrug efflux system protein MdtJ 2163685..2164050 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157152 YP_003036276.1 CDS ECBD_2047 NC_012947.1 2164037 2164366 D with MdtJ is involved in resistance to deoxycholate, nalidixic acid, fosfomycin, and SDS; multidrug efflux system protein MdtI 2164037..2164366 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157153 YP_003036277.1 CDS ECBD_2048 NC_012947.1 2164405 2165226 R PFAM: peptidase S1 and S6 chymotrypsin/Hap; KEGG: elf:LF82_2843 uncharacterized serine protease YdgD; peptidase S1 and S6 chymotrypsin/Hap complement(2164405..2165226) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157154 YP_003036278.1 CDS ECBD_2049 NC_012947.1 2165502 2165810 R required for growth and survival under moderately acid conditions; acid shock protein precursor complement(2165502..2165810) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157155 YP_003036279.1 CDS ECBD_2050 NC_012947.1 2166234 2167487 R PFAM: major facilitator superfamily MFS_1; KEGG: ssn:SSON_1568 transporter; major facilitator superfamily MFS_1 complement(2166234..2167487) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157156 YP_003036280.1 CDS ECBD_2051 NC_012947.1 2167594 2168487 D PFAM: LysR substrate-binding; regulatory protein LysR; LysR family transcriptional regulator 2167594..2168487 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157157 YP_003036281.1 CDS ECBD_2052 NC_012947.1 2168622 2169842 D PFAM: ROK family protein; KEGG: sbc:SbBS512_E1780 transcriptional regulator Mic; ROK family protein 2168622..2169842 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157158 YP_003036282.1 CDS ECBD_2053 NC_012947.1 2169967 2170662 D DTB synthetase; dethiobiotin synthase; involved in production of dethiobiotin from ATP and 7,8-diaminononanoate and carbon dioxide; contains magnesium; dithiobiotin synthetase 2169967..2170662 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157159 YP_003036283.1 CDS ECBD_2054 NC_012947.1 2170615 2171871 R PFAM: chloride channel core; voltage-gated ClC-type chloride channel ClcB complement(2170615..2171871) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157160 YP_003036284.1 CDS ECBD_2055 NC_012947.1 2172066 2172680 R binds to the twin-arginine signal peptides of certain proteins, including dimethylsulfoxide reductase and trimethylamine N-oxide reductase that are translocated to the cytoplasmic membrane; twin-argninine leader-binding protein DmsD complement(2172066..2172680) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157161 YP_003036285.1 CDS ECBD_2056 NC_012947.1 2172723 2173577 R PFAM: DMSO reductase anchor subunit (DmsC); KEGG: sbo:SBO_1546 DMSO reductase anchor subunit; DMSO reductase anchor subunit (DmsC) complement(2172723..2173577) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157162 YP_003036286.1 CDS ECBD_2057 NC_012947.1 2173579 2174196 R TIGRFAM: dimethylsulfoxide reductase, chain B; PFAM: 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: sdy:SDY_1567 oxidoreductase, Fe-S subunit; dimethylsulfoxide reductase, chain B complement(2173579..2174196) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157163 YP_003036287.1 CDS ECBD_2058 NC_012947.1 2174207 2176630 R TIGRFAM: anaerobic dimethyl sulfoxide reductase, A subunit, DmsA/YnfE family; PFAM: molybdopterin oxidoreductase; molybdopterin oxidoreductase Fe4S4 region; molydopterin dinucleotide-binding region; KEGG: sbo:SBO_1548 oxidoreductase, major subunit; anaerobic dimethyl sulfoxide reductase, subunit alpha, DmsA/YnfE family complement(2174207..2176630) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157164 YP_003036288.1 CDS ECBD_2059 NC_012947.1 2176691 2179117 R TIGRFAM: anaerobic dimethyl sulfoxide reductase, A subunit, DmsA/YnfE family; PFAM: molybdopterin oxidoreductase; molybdopterin oxidoreductase Fe4S4 region; molydopterin dinucleotide-binding region; anaerobic dimethyl sulfoxide reductase, subunit alpha, DmsA/YnfE family complement(2176691..2179117) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157165 YP_003036289.1 CDS ECBD_2060 NC_012947.1 2179316 2179621 R PFAM: protein of unknown function DUF1161; hypothetical protein complement(2179316..2179621) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157166 YP_003036290.1 CDS ECBD_2061 NC_012947.1 2179693 2180439 D PFAM: protein of unknown function UPF0257; KEGG: sfv:SFV_1559 hypothetical protein; hypothetical protein 2179693..2180439 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157167 YP_003036291.1 CDS ECBD_2062 NC_012947.1 2180442 2181002 R PFAM: GCN5-related N-acetyltransferase; diamine N-acetyltransferase complement(2180442..2181002) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157168 YP_003036292.1 CDS ECBD_2063 NC_012947.1 2181037 2181378 R PFAM: protein of unknown function DUF1283; KEGG: sfv:SFV_1561 hypothetical protein; hypothetical protein complement(2181037..2181378) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157169 YP_003036293.1 CDS ECBD_2064 NC_012947.1 2181513 2181839 D PFAM: protein of unknown function UPF0060; KEGG: elf:LF82_3567 UPF0060 membrane protein YnfA; hypothetical protein 2181513..2181839 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157170 YP_003036294.1 CDS ECBD_2065 NC_012947.1 2182045 2183259 D PFAM: mandelate racemase/muconate lactonizing protein; mandelate racemase/muconate lactonizing protein 2182045..2183259 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157171 YP_003036295.1 CDS ECBD_2066 NC_012947.1 2183271 2184290 D PFAM: alcohol dehydrogenase GroES domain protein; alcohol dehydrogenase zinc-binding domain protein; KEGG: elf:LF82_2029 starvation-sensing protein RspB; dehydrogenase 2183271..2184290 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157172 YP_003036296.1 CDS ECBD_2069 NC_012947.1 2185986 2186492 R KEGG: elf:LF82_2828 ExoO exonuclease VIII, ds DNA exonuclease encoded by prophage CP-933O; ExoO exonuclease VIII, ds DNA exonuclease encoded by prophage CP-933O complement(2185986..2186492) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157175 YP_003036297.1 CDS ECBD_2070 NC_012947.1 2186586 2186777 R PFAM: protein of unknown function DUF1482; KEGG: elf:LF82_2827 uncharacterized protein YdfD; hypothetical protein complement(2186586..2186777) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157176 YP_003036298.1 CDS ECBD_2071 NC_012947.1 2186774 2186962 R PFAM: DicB family protein; KEGG: elf:LF82_0484 division inhibition protein DicB; DicB family protein complement(2186774..2186962) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157177 YP_003036299.1 CDS ECBD_2072 NC_012947.1 2187465 2187665 D hypothetical protein 2187465..2187665 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157178 YP_003036300.1 CDS ECBD_2073 NC_012947.1 2187634 2187999 R hypothetical protein complement(2187634..2187999) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157179 YP_003036301.1 CDS ECBD_2075 NC_012947.1 2188332 2188739 R The transcriptional repressor of DicA (divison cell) has a hypothetical DNA binding domain. It represses the transcription of dicB and dicC, but its specific role in cellular replication and mode of action are still unknown.; transcriptional repressor DicA complement(2188332..2188739) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158241 YP_003036302.1 CDS ECBD_2076 NC_012947.1 2188820 2189047 D DNA-binding transcriptional regulator DicC 2188820..2189047 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159564 YP_003036303.1 CDS ECBD_2077 NC_012947.1 2189031 2189552 D PFAM: protein of unknown function DUF1019; hypothetical protein 2189031..2189552 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159565 YP_003036304.1 CDS ECBD_2078 NC_012947.1 2189533 2190498 D KEGG: sbc:SbBS512_E1433 phage O protein family; phage O protein family 2189533..2190498 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159566 YP_003036305.1 CDS ECBD_2079 NC_012947.1 2190539 2190898 D PFAM: protein of unknown function DUF977; KEGG: sbc:SbBS512_E1436 hypothetical protein; hypothetical protein 2190539..2190898 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159567 YP_003036306.1 CDS ECBD_2081 NC_012947.1 2191103 2191315 D PFAM: Hok/gef cell toxic protein; KEGG: sfx:S2143 cell killing protein of prophage; Hok/gef cell toxic protein 2191103..2191315 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159569 YP_003036307.1 CDS ECBD_2082 NC_012947.1 2191532 2191783 D KEGG: sfv:SFV_1543 hypothetical protein; hypothetical protein 2191532..2191783 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159570 YP_003036308.1 CDS ECBD_2083 NC_012947.1 2191850 2192128 D KEGG: elf:LF82_p279 hypothetical protein; hypothetical protein 2191850..2192128 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159571 YP_003036309.1 CDS ECBD_2084 NC_012947.1 2192130 2192780 D PFAM: protein of unknown function DUF1277; SMART: HNH nuclease; KEGG: elf:LF82_2839 uncharacterized protein YdfU; hypothetical protein 2192130..2192780 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159572 YP_003036310.1 CDS ECBD_2085 NC_012947.1 2192798 2193175 D PFAM: antitermination Q family protein; KEGG: efe:EFER_2706 antitermination protein Q homolog; DLP12 prophage; antitermination Q family protein 2192798..2193175 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159573 YP_003036311.1 CDS ECBD_2086 NC_012947.1 2193331 2193855 R lipocalin; globomycin-sensitive outer membrane lipoprotein; outer membrane lipoprotein Blc complement(2193331..2193855) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159574 YP_003036312.1 CDS ECBD_2087 NC_012947.1 2194048 2194197 D KEGG: efe:EFER_2704 conserved hypothetical protein from bacteriophage origin; hypothetical protein 2194048..2194197 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159575 YP_003036313.1 CDS ECBD_2088 NC_012947.1 2194225 2195091 R PFAM: Integrase catalytic region; KEGG: sfv:SFV_1039 IS3 ORF2; Integrase catalytic region complement(2194225..2195091) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159576 YP_003036314.1 CDS ECBD_2089 NC_012947.1 2195088 2195387 R PFAM: transposase IS3/IS911 family protein; KEGG: sfx:S3209 IS3 orfA; transposase IS3/IS911 family protein complement(2195088..2195387) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159577 YP_003036315.1 CDS ECBD_2091 NC_012947.1 2197349 2197630 D KEGG: elf:LF82_p182 hypothetical protein; hypothetical protein 2197349..2197630 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159579 YP_003036316.1 CDS ECBD_2092 NC_012947.1 2197640 2198344 D hypothetical protein 2197640..2198344 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159580 YP_003036317.1 CDS ECBD_2093 NC_012947.1 2198355 2198648 D KEGG: elf:LF82_p259 hypothetical protein; hypothetical protein 2198355..2198648 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159581 YP_003036318.1 CDS ECBD_2094 NC_012947.1 2198876 2199466 R PFAM: Resolvase domain; Resolvase helix-turn-helix domain protein; KEGG: elf:LF82_1666 DNA-invertase from lambdoid prophage Rac; resolvase complement(2198876..2199466) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159582 YP_003036319.1 CDS ECBD_2095 NC_012947.1 2199782 2200015 R KEGG: elf:LF82_2833 uncharacterized protein YdfK; hypothetical protein complement(2199782..2200015) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159583 YP_003036320.1 CDS ECBD_2096 NC_012947.1 2200801 2202084 D TIGRFAM: metabolite/H+ symporter, major facilitator superfamily (MFS); PFAM: major facilitator superfamily MFS_1; general substrate transporter; KEGG: elf:LF82_2832 inner membrane metabolite transport protein YdfJ; metabolite/H+ symporter, major facilitator superfamily (MFS) 2200801..2202084 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159584 YP_003036321.1 CDS ECBD_2097 NC_012947.1 2202173 2203633 D PFAM: Mannitol dehydrogenase domain; Mannitol dehydrogenase rossman domain; KEGG: sfx:S1678 oxidoreductase; mannitol dehydrogenase 2202173..2203633 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159585 YP_003036322.1 CDS ECBD_2098 NC_012947.1 2203669 2203872 R TIGRFAM: selenium-binding protein YdfZ; KEGG: sdy:SDY_1584 hypothetical protein; selenium-binding protein YdfZ complement(2203669..2203872) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159586 YP_003036323.1 CDS ECBD_2099 NC_012947.1 2204049 2204735 R PFAM: GntR family transcriptional regulator; regulatory protein GntR HTH; SMART: regulatory protein GntR HTH; KEGG: ssn:SSON_1588 hypothetical protein; GntR family transcriptional regulator complement(2204049..2204735) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159587 YP_003036324.1 CDS ECBD_2100 NC_012947.1 2204824 2205570 R NADP(+)-dependent; catalyzes the formation of 2-aminomalonate-semialdehyde from L-serine; can also use 3-hydroxybutyrate, 3-hydroxy-isobutyrate, D-threonine, L-allo-threonine,D-serine; 3-hydroxy acid dehydrogenase complement(2204824..2205570) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159588 YP_003036325.1 CDS ECBD_2101 NC_012947.1 2205641 2207752 D PFAM: peptidase M3A and M3B thimet/oligopeptidase F; dipeptidyl carboxypeptidase II 2205641..2207752 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159589 YP_003036326.1 CDS ECBD_2102 NC_012947.1 2207796 2208314 R PFAM: damage inducible protein CinA; KEGG: sdy:SDY_1588 competence damage-inducible protein A; competence damage-inducible protein A complement(2207796..2208314) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159590 YP_003036327.1 CDS ECBD_2103 NC_012947.1 2208590 2208982 D PFAM: conserved hypothetical protein; KEGG: sbc:SbBS512_E1704 hypothetical protein; hypothetical protein 2208590..2208982 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159591 YP_003036328.1 CDS ECBD_2104 NC_012947.1 2209237 2210127 D KEGG: elf:LF82_2817 uncharacterized protein YdeH; TIGRFAM: diguanylate cyclase; PFAM: GGDEF domain containing protein; SMART: GGDEF domain containing protein; hypothetical protein 2209237..2210127 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159592 YP_003036329.1 CDS ECBD_2105 NC_012947.1 2210346 2210441 R KEGG: sea:SeAg_B1655 hypothetical protein; hypothetical protein complement(2210346..2210441) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159593 YP_003036330.1 CDS ECBD_2106 NC_012947.1 2210568 2211755 R YdeF; uncharacterized member of the major facilitator superfamily (MFS) of transporters; MFS-type transporter YdeE complement(2210568..2211755) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159594 YP_003036331.1 CDS ECBD_2107 NC_012947.1 2211950 2212849 D PFAM: protein of unknown function DUF6 transmembrane; protein of unknown function DUF250; KEGG: sfv:SFV_1593 O-acetylserine/cysteine export protein; O-acetylserine/cysteine export protein 2211950..2212849 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159595 YP_003036332.1 CDS ECBD_2108 NC_012947.1 2212880 2213098 R KEGG: ssn:SSON_1596 hypothetical protein; hypothetical protein complement(2212880..2213098) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159596 YP_003036333.1 CDS ECBD_2109 NC_012947.1 2213130 2213513 R transcriptional activator of genes involved in the multiple antibiotic resistance (Mar) phenotype; also activates sodA, zwf and micF; DNA-binding transcriptional activator MarA complement(2213130..2213513) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159597 YP_003036334.1 CDS ECBD_2110 NC_012947.1 2213533 2213967 R Repressor of the marRAB operon which is involved in the activation of both antibiotic resistance and oxidative stress genes; DNA-binding transcriptional repressor MarR complement(2213533..2213967) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159598 YP_003036335.1 CDS ECBD_2111 NC_012947.1 2214187 2214507 D PFAM: protein of unknown function DUF891; KEGG: esa:ESA_03838 hypothetical protein; hypothetical protein 2214187..2214507 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159599 YP_003036336.1 CDS ECBD_2112 NC_012947.1 2214497 2214781 D PFAM: helix-turn-helix domain protein; SMART: helix-turn-helix domain protein; KEGG: plu:plu0900 hypothetical protein; XRE family transcriptional regulator 2214497..2214781 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159600 YP_003036337.1 CDS ECBD_2113 NC_012947.1 2214902 2215567 D protein involved in resistance to different drugs (tetracycline, chloramphenicol, beta-lactams, and quinolones); part of the multiple antibiotic resistance (mar) locus, which is composed by the genes marC and marRAB; unknown function; multiple drug resistance protein MarC 2214902..2215567 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159601 YP_003036338.1 CDS ECBD_2114 NC_012947.1 2215592 2216782 R PFAM: major facilitator superfamily MFS_1; KEGG: sbo:SBO_1646 sugar efflux transporter; sugar efflux transporter complement(2215592..2216782) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159602 YP_003036339.1 CDS ECBD_2115 NC_012947.1 2216932 2218047 R KEGG: ssn:SSON_1601 hypothetical protein; hypothetical protein complement(2216932..2218047) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159603 YP_003036340.1 CDS ECBD_2116 NC_012947.1 2218125 2219006 R PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: elf:LF82_3566 uncharacterized HTH-type transcriptional regulator; LysR family transcriptional regulator complement(2218125..2219006) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159604 YP_003036341.1 CDS ECBD_2117 NC_012947.1 2219107 2220495 D in Escherichia coli this enzyme appears to be an NAD+/NADP+-dependent succinate semialdehyde dehydrogenase; succinate semialdehyde dehydrogenase 2219107..2220495 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159605 YP_003036342.1 CDS ECBD_2118 NC_012947.1 2220559 2221485 D catalyzes the formation of glutamate from glutamine; glutaminase 2220559..2221485 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159606 YP_003036343.1 CDS ECBD_2119 NC_012947.1 2221485 2221844 D KEGG: sfv:SFV_1582 hypothetical protein; hypothetical protein 2221485..2221844 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159607 YP_003036344.1 CDS ECBD_2120 NC_012947.1 2221983 2223401 D KEGG: sfv:SFV_1581 hypothetical protein; TIGRFAM: diguanylate cyclase; PFAM: GGDEF domain containing protein; SMART: GGDEF domain containing protein; diguanylate cyclase 2221983..2223401 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159608 YP_003036345.1 CDS ECBD_2121 NC_012947.1 2223628 2225079 D catalyzes the formation of D-tagaturonate from D-altronate; altronate oxidoreductase 2223628..2225079 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159609 YP_003036346.1 CDS ECBD_2122 NC_012947.1 2225286 2226200 D PFAM: protein of unknown function UPF0187; KEGG: sbc:SbBS512_E1689 hypothetical protein; hypothetical protein 2225286..2226200 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159610 YP_003036347.1 CDS ECBD_2123 NC_012947.1 2226204 2226962 R catalyzes the formation of (E)-3-(methoxycarbonyl)pent-2-enedioate and S-adenosyl-L-homocysteine from S-adenosyl-L-methionine and trans-aconitate; trans-aconitate 2-methyltransferase complement(2226204..2226962) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159611 YP_003036348.1 CDS ECBD_2124 NC_012947.1 2227019 2227309 R PFAM: antibiotic biosynthesis monooxygenase; KEGG: sdy:SDY_1624 autoinducer-2 (AI-2) modifying protein LsrG; autoinducer-2 (AI-2) modifying protein LsrG complement(2227019..2227309) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159612 YP_003036349.1 CDS ECBD_2125 NC_012947.1 2227333 2228208 R PFAM: deoxyribose-phosphate aldolase/phospho-2-dehydro-3-deoxyheptonate aldolase; KEGG: efe:EFER_1560 aldolase; aldolase complement(2227333..2228208) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159613 YP_003036350.1 CDS ECBD_2126 NC_012947.1 2228235 2229257 R sugar ABC transporter substrate-binding protein complement(2228235..2229257) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159614 YP_003036351.1 CDS ECBD_2128 NC_012947.1 2230071 2231621 D TIGRFAM: outer membrane autotransporter barrel domain protein; PFAM: Autotransporter beta- domain protein; Pertactin; outer membrane autotransporter barrel domain protein 2230071..2231621 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159616 YP_003036352.1 CDS ECBD_2129 NC_012947.1 2231649 2231747 D hypothetical protein 2231649..2231747 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159617 YP_003036353.1 CDS ECBD_2130 NC_012947.1 2231844 2232110 D PFAM: helix-turn-helix domain protein; SMART: helix-turn-helix domain protein; KEGG: ssn:SSON_1613 DNA-binding transcriptional regulator; DNA-binding transcriptional regulator HipB 2231844..2232110 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159618 YP_003036354.1 CDS ECBD_2131 NC_012947.1 2232110 2233432 D TIGRFAM: HipA N-terminal domain protein; PFAM: HipA domain protein; KEGG: elf:LF82_0996 protein HipA; HipA N-terminal domain protein 2232110..2233432 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159619 YP_003036355.1 CDS ECBD_2132 NC_012947.1 2233804 2234286 D PFAM: fimbrial protein; KEGG: elf:LF82_p244 Fml fimbriae subunit; fimbrial protein 2233804..2234286 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159620 YP_003036356.1 CDS ECBD_2135 NC_012947.1 2238063 2238593 D PFAM: fimbrial protein; fimbrial protein 2238063..2238593 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159623 YP_003036357.1 CDS ECBD_2136 NC_012947.1 2238606 2239109 D PFAM: fimbrial protein; fimbrial protein 2238606..2239109 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159624 YP_003036358.1 CDS ECBD_2137 NC_012947.1 2239168 2240082 D PFAM: FimH mannose-binding domain protein; fimbrial protein; KEGG: ssn:SSON_1621 adhesin; FimH mannose-binding domain protein 2239168..2240082 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159625 YP_003036359.1 CDS ECBD_2138 NC_012947.1 2240416 2242695 D in Escherichia coli it may be involved in repression of type III secretion system (TTSS) genes of the host cell; involved with increased resistance to low pH; member of the prokaryotic molybdopterin-containing oxidoreductase family; oxidoreductase 2240416..2242695 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159626 YP_003036360.1 CDS ECBD_2139 NC_012947.1 2242943 2243140 D KEGG: sfx:S1655 hypothetical protein; hypothetical protein 2242943..2243140 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159627 YP_003036361.1 CDS ECBD_2140 NC_012947.1 2243215 2243976 D regulates the cellular response to acid resistance; transcriptional regulator YdeO 2243215..2243976 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159628 YP_003036362.1 CDS ECBD_2141 NC_012947.1 2244378 2246060 D PFAM: sulfatase; sulfatase 2244378..2246060 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159629 YP_003036363.1 CDS ECBD_2142 NC_012947.1 2246112 2247269 D PFAM: radical SAM protein; KEGG: sfv:SFV_1723 enzyme; radical SAM protein 2246112..2247269 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159630 YP_003036364.1 CDS ECBD_2143 NC_012947.1 2247560 2249245 D PFAM: ABC transporter; ABC transporter domain protein; SbmABacA family protein; SMART: ATPase AAA; KEGG: ssn:SSON_1627 ATP-binding component of a transporter; ABC transporter 2247560..2249245 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159631 YP_003036365.1 CDS ECBD_2144 NC_012947.1 2249283 2251655 D PFAM: TonB-dependent receptor plug; TonB-dependent receptor; KEGG: ssn:SSON_1629 hypothetical protein; TonB-dependent receptor plug 2249283..2251655 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159632 YP_003036366.1 CDS ECBD_2145 NC_012947.1 2251712 2254495 D PFAM: peptidase M16 domain protein; KEGG: sbo:SBO_1562 peptidase; peptidase M16 domain protein 2251712..2254495 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159633 YP_003036367.1 CDS ECBD_2146 NC_012947.1 2254857 2256257 D TIGRFAM: glutamate decarboxylase; PFAM: pyridoxal-dependent decarboxylase; KEGG: sbo:SBO_1563 glutamate decarboxylase isozyme; glutamate decarboxylase 2254857..2256257 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159634 YP_003036368.1 CDS ECBD_2147 NC_012947.1 2256413 2257948 D TIGRFAM: glutamate/g-aminobutyrate antiporter; PFAM: amino acid permease-associated region; KEGG: sbc:SbBS512_E1755 glutamate:gamma aminobutyrate antiporter; glutamate/g-aminobutyrate antiporter 2256413..2257948 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159635 YP_003036369.1 CDS ECBD_2148 NC_012947.1 2258079 2259398 D PFAM: protein of unknown function DUF187; KEGG: ssn:SSON_1633 hypothetical protein; hypothetical protein 2258079..2259398 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159636 YP_003036370.1 CDS ECBD_2149 NC_012947.1 2259775 2261157 D KEGG: ssn:SSON_1634 hypothetical protein; TIGRFAM: diguanylate cyclase; PFAM: GGDEF domain containing protein; SMART: GGDEF domain containing protein; diguanylate cyclase 2259775..2261157 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159637 YP_003036371.1 CDS ECBD_2150 NC_012947.1 2261182 2263581 D heme-regulated phosphodiesterase with phosphodiesterase activity with cAMP but not with cGMP, bis(p-nitrophenyl) phosphate or p-nitrophenyl phosphate; cAMP phosphodiesterase 2261182..2263581 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159638 YP_003036372.1 CDS ECBD_2151 NC_012947.1 2263839 2264420 D catalyzes the hydrolysis of D-alanyl-D-alanine dipeptide: Zn dependent; involved in peptidoglycan synthesis; D-alanyl-D-alanine dipeptidase 2263839..2264420 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159639 YP_003036373.1 CDS ECBD_2152 NC_012947.1 2264434 2265984 D PFAM: extracellular solute-binding protein family 5; extracellular solute-binding protein family 5 2264434..2265984 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159640 YP_003036374.1 CDS ECBD_2153 NC_012947.1 2265986 2267008 D PFAM: binding-protein-dependent transporters inner membrane component; KEGG: sbo:SBO_1571 transporter permease protein; binding-protein-dependent transporters inner membrane component 2265986..2267008 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159641 YP_003036375.1 CDS ECBD_2154 NC_012947.1 2267005 2267901 D PFAM: binding-protein-dependent transporters inner membrane component; KEGG: ssn:SSON_1639 ATP-dependent peptide transporter membrane subunit; ATP-dependent peptide transporter membrane subunit 2267005..2267901 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159642 YP_003036376.1 CDS ECBD_2155 NC_012947.1 2267898 2268884 D KEGG: sbc:SbBS512_E1747 ABC transporter ATP-binding protein; TIGRFAM: oligopeptide/dipeptide ABC transporter ATPase; PFAM: ABC transporter; oligopeptide/dipeptide ABC transporter domain protein; SMART: ATPase AAA; peptide ABC transporter ATPase 2267898..2268884 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159643 YP_003036377.1 CDS ECBD_2156 NC_012947.1 2268877 2269803 D KEGG: sbo:SBO_1574 ATP-binding component of a transporter; TIGRFAM: oligopeptide/dipeptide ABC transporter ATPase subunit; PFAM: ABC transporter; oligopeptide/dipeptide ABC transporter domain protein; SMART: ATPase AAA; peptide ABC transporter ATPase 2268877..2269803 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159885 YP_003036378.1 CDS ECBD_2157 NC_012947.1 2269859 2270290 R PFAM: OsmC family protein; KEGG: sfv:SFV_1739 osmotically inducible protein; peroxiredoxin complement(2269859..2270290) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157263 YP_003036379.1 CDS ECBD_2158 NC_012947.1 2270635 2270850 D KEGG: efe:EFER_1498 biofilm-dependent modulation protein; biofilm-dependent modulation protein 2270635..2270850 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157264 YP_003036380.1 CDS rpsV NC_012947.1 2270952 2271089 D protein D; stationary-phase-induced ribosome-associated; in Escherichia coli this protein becomes associated with the ribosome; expression increases during stationary phase; 30S ribosomal subunit S22 2270952..2271089 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157265 YP_003036381.1 CDS ECBD_2160 NC_012947.1 2271246 2272943 D malic enzyme; oxaloacetate-decarboxylating; NAD-dependent; catalyzes the formation of pyruvate form malate; malate dehydrogenase 2271246..2272943 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157266 YP_003036382.1 CDS ECBD_2161 NC_012947.1 2273077 2274087 D similar to zinc-dependent eukaryotic ADH enzymes and distinct from fermentative ADHs; alcohol dehydrogenase 2273077..2274087 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157267 YP_003036383.1 CDS ECBD_2162 NC_012947.1 2274233 2274517 D KEGG: ssn:SSON_1647 hypothetical protein; TIGRFAM: addiction module antidote protein, HigA family; PFAM: helix-turn-helix domain protein; SMART: helix-turn-helix domain protein; XRE family transcriptional regulator 2274233..2274517 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157268 YP_003036384.1 CDS ECBD_2163 NC_012947.1 2274712 2275365 R nitrate-inducible, cytochrome b556(fdn) component of formate dehydrogenase; formate dehydrogenase-N subunit gamma complement(2274712..2275365) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157269 YP_003036385.1 CDS ECBD_2164 NC_012947.1 2275358 2276242 R TIGRFAM: formate dehydrogenase subunit beta; PFAM: formate dehydrogenase transmembrane domain protein; 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: sfv:SFV_1746 formate dehydrogenase-N, nitrate-inducible, iron-sulfur subunit beta; formate dehydrogenase subunit beta complement(2275358..2276242) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157270 YP_003036386.1 CDS ECBD_2165 NC_012947.1 2276255 2279302 R KEGG: ssn:SSON_1650 formate dehydrogenase-N, nitrate-inducible, subunit alpha; TIGRFAM: formate dehydrogenase, alpha subunit; PFAM: molybdopterin oxidoreductase; molybdopterin oxidoreductase Fe4S4 region; molydopterin dinucleotide-binding region; formate dehydrogenase, subunit alpha complement(2276255..2279302) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157271 YP_003036387.1 CDS ECBD_2166 NC_012947.1 2279591 2280415 D PFAM: protein of unknown function DUF6 transmembrane; KEGG: elf:LF82_2811 inner membrane protein YddG; hypothetical protein 2279591..2280415 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157272 YP_003036388.1 CDS ECBD_2167 NC_012947.1 2280674 2280964 D KEGG: sbo:SBO_1585 outer membrane porin protein; outer membrane porin protein 2280674..2280964 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157273 YP_003036389.1 CDS ECBD_2169 NC_012947.1 2282642 2284030 D TIGRFAM: nitrite transporter; PFAM: major facilitator superfamily MFS_1; KEGG: elf:LF82_1467 nitrite extrusion protein 2; nitrite transporter 2282642..2284030 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157275 YP_003036390.1 CDS ECBD_2170 NC_012947.1 2284112 2287852 D TIGRFAM: nitrate reductase, subunit alpha; PFAM: molybdopterin oxidoreductase; molydopterin dinucleotide-binding region; KEGG: sfv:SFV_1757 cryptic nitrate reductase 2, subunit alpha; nitrate reductase, subunit alpha 2284112..2287852 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157276 YP_003036391.1 CDS ECBD_2171 NC_012947.1 2287849 2289393 D TIGRFAM: nitrate reductase subunit beta; nitrate reductase subunit beta 2287849..2289393 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157277 YP_003036392.1 CDS ECBD_2172 NC_012947.1 2289393 2290088 D KEGG: ssn:SSON_1659 cryptic nitrate reductase 2, delta subunit, assembly function; TIGRFAM: nitrate reductase molybdenum cofactor assembly chaperone; PFAM: nitrate reductase subunit delta; nitrate reductase molybdenum cofactor assembly chaperone 2289393..2290088 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157278 YP_003036393.1 CDS ECBD_2173 NC_012947.1 2290085 2290765 D TIGRFAM: respiratory nitrate reductase, gamma subunit; PFAM: nitrate reductase subunit gamma; respiratory nitrate reductase, subunit gamma 2290085..2290765 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157279 YP_003036394.1 CDS ECBD_2175 NC_012947.1 2291833 2292678 R PFAM: N-acetyltransferase; KEGG: ssn:SSON_1662 N-hydroxyarylamine O-acetyltransferase; N-hydroxyarylamine O-acetyltransferase complement(2291833..2292678) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157281 YP_003036395.1 CDS ECBD_2176 NC_012947.1 2292851 2293420 D PFAM: flavin reductase domain protein FMN-binding; KEGG: elf:LF82_2812 uncharacterized protein YddH; flavin reductase domain protein FMN-binding 2292851..2293420 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157282 YP_003036396.1 CDS ECBD_2177 NC_012947.1 2293424 2293651 R 4-OT; member of subfamily 5; forms a dimer; the function in the Escherichia coli cell is unknown; 4-oxalocrotonate tautomerase complement(2293424..2293651) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157283 YP_003036397.1 CDS ECBD_2178 NC_012947.1 2293684 2294475 R PFAM: transposase IS4 family protein; KEGG: ssn:SSON_0260 receptor; transposase IS4 family protein complement(2293684..2294475) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157284 YP_003036398.1 CDS ECBD_2180 NC_012947.1 2295887 2296369 R hypothetical protein complement(2295887..2296369) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157286 YP_003036399.1 CDS ECBD_2182 NC_012947.1 2299990 2300346 R KEGG: ssn:SSON_0520 Vgr protein; Vgr protein complement(2299990..2300346) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157288 YP_003036400.1 CDS ECBD_2183 NC_012947.1 2301166 2301378 R KEGG: sdy:SDY_1950 hypothetical protein; hypothetical protein complement(2301166..2301378) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157289 YP_003036401.1 CDS ECBD_2184 NC_012947.1 2301454 2302071 R KEGG: sbo:SBO_1593 transferase; transferase complement(2301454..2302071) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157290 YP_003036402.1 CDS ECBD_2185 NC_012947.1 2302119 2302268 R KEGG: sbc:SbBS512_E1726 hypothetical protein; hypothetical protein complement(2302119..2302268) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157291 YP_003036403.1 CDS ECBD_2186 NC_012947.1 2302338 2303837 D PFAM: amino acid permease-associated region; KEGG: sbc:SbBS512_E1725 L-asparagine permease; amino acid permease-associated region 2302338..2303837 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157292 YP_003036404.1 CDS ECBD_2187 NC_012947.1 2303952 2305013 R KEGG: sbc:SbBS512_E1724 hypothetical protein; hypothetical protein complement(2303952..2305013) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157293 YP_003036405.1 CDS ECBD_2188 NC_012947.1 2305255 2307357 D PFAM: TonB-dependent receptor; TonB-dependent receptor plug; KEGG: ssn:SSON_1682 outer membrane receptor for iron transport; TonB-dependent receptor 2305255..2307357 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157294 YP_003036406.1 CDS ECBD_2189 NC_012947.1 2307393 2308058 R PFAM: GntR family transcriptional regulator; regulatory protein GntR HTH; SMART: regulatory protein GntR HTH; KEGG: sfv:SFV_1761 DNA-binding transcriptional regulator; DNA-binding transcriptional regulator complement(2307393..2308058) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157295 YP_003036407.1 CDS ECBD_2190 NC_012947.1 2308256 2309293 R PFAM: alcohol dehydrogenase zinc-binding domain protein; KEGG: sfv:SFV_1762 oxidoreductase; alcohol dehydrogenase zinc-binding domain protein complement(2308256..2309293) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157296 YP_003036408.1 CDS ECBD_2191 NC_012947.1 2309474 2309992 D PFAM: GCN5-related N-acetyltransferase; KEGG: sbc:SbBS512_E1718 acetyltransferase, GNAT family; GCN5-related N-acetyltransferase 2309474..2309992 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157297 YP_003036409.1 CDS ECBD_2192 NC_012947.1 2309989 2310438 D PFAM: protein of unknown function DUF606; KEGG: sbo:SBO_1601 hypothetical protein; hypothetical protein 2309989..2310438 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157298 YP_003036410.1 CDS ECBD_2193 NC_012947.1 2310439 2310672 R KEGG: sfl:SF1771 hypothetical protein; hypothetical protein complement(2310439..2310672) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157299 YP_003036411.1 CDS ECBD_2194 NC_012947.1 2310758 2310931 R KEGG: sdy:SDY_1729 hypothetical protein; hypothetical protein complement(2310758..2310931) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157300 YP_003036412.1 CDS ECBD_2195 NC_012947.1 2311318 2312742 R catalyzes the formation of 4-aminobutanoate from 4-aminobutanal; involved in putrescine degradation; gamma-aminobutyraldehyde dehydrogenase complement(2311318..2312742) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157301 YP_003036413.1 CDS ECBD_2196 NC_012947.1 2312764 2313558 R PFAM: binding-protein-dependent transporters inner membrane component; KEGG: sfv:SFV_1771 transporter permease protein; binding-protein-dependent transporters inner membrane component complement(2312764..2313558) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157302 YP_003036414.1 CDS ECBD_2197 NC_012947.1 2313548 2314489 R PFAM: binding-protein-dependent transporters inner membrane component; binding-protein-dependent transporters inner membrane component complement(2313548..2314489) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157303 YP_003036415.1 CDS ECBD_2198 NC_012947.1 2314490 2315503 R PFAM: ABC transporter; transport-associated OB domain protein; SMART: ATPase AAA; KEGG: ssn:SSON_1696 ATP-binding component of a transporter; ABC transporter complement(2314490..2315503) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157304 YP_003036416.1 CDS ECBD_2199 NC_012947.1 2315521 2316666 R PFAM: extracellular solute-binding protein family 1; KEGG: ssn:SSON_1697 transporter; extracellular solute-binding protein family 1 complement(2315521..2316666) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157305 YP_003036417.1 CDS ECBD_2200 NC_012947.1 2316911 2318317 R PFAM: regulatory protein GntR HTH; aminotransferase class I and II; SMART: regulatory protein GntR HTH; KEGG: ssn:SSON_1698 multi modular; transcriptional regulator; also ATP-binding component of a transporter; GntR family transcriptional regulator complement(2316911..2318317) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157306 YP_003036418.1 CDS ECBD_2201 NC_012947.1 2318396 2318833 R PFAM: protein of unknown function UPF0150; helix-turn-helix domain protein; SMART: helix-turn-helix domain protein; KEGG: sdy:SDY_1736 hypothetical protein; XRE family transcriptional regulator complement(2318396..2318833) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157307 YP_003036419.1 CDS ECBD_2202 NC_012947.1 2318858 2319034 R PFAM: YcfA family protein; KEGG: cko:CKO_01475 hypothetical protein; YcfA family protein complement(2318858..2319034) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157308 YP_003036420.1 CDS ECBD_2203 NC_012947.1 2319316 2319486 D KEGG: ssn:SSON_1702 hypothetical protein; hypothetical protein 2319316..2319486 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157309 YP_003036421.1 CDS ECBD_2204 NC_012947.1 2319578 2321539 R PFAM: peptidase U32; peptidase U32 complement(2319578..2321539) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157310 YP_003036422.1 CDS ECBD_2205 NC_012947.1 2321612 2322148 R PFAM: helix-turn-helix domain protein; Cupin 2 conserved barrel domain protein; SMART: helix-turn-helix domain protein; KEGG: ssn:SSON_1704 hypothetical protein; XRE family transcriptional regulator complement(2321612..2322148) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157311 YP_003036423.1 CDS ECBD_2206 NC_012947.1 2322240 2323415 D TIGRFAM: benzoate transporter; PFAM: Benzoate membrane transporter; xanthine/uracil/vitamin C permease; benzoate transporter 2322240..2323415 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157312 YP_003036424.1 CDS ECBD_2207 NC_012947.1 2323455 2324663 R TIGRFAM: transposase, IS605 OrfB family; PFAM: transposase IS891/IS1136/IS1341 family; transposase IS605 OrfB; KEGG: ssn:SSON_1709 virulence protein; transposase, IS605 OrfB family complement(2323455..2324663) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157313 YP_003036425.1 CDS ECBD_2209 NC_012947.1 2325195 2325863 R KEGG: elf:LF82_2797 uncharacterized lipoprotein YdcL; hypothetical protein complement(2325195..2325863) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157315 YP_003036426.1 CDS ECBD_2210 NC_012947.1 2326166 2326759 R with TehA confers resistance to tellurite; tellurite resistance protein TehB complement(2326166..2326759) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157316 YP_003036427.1 CDS ECBD_2211 NC_012947.1 2326756 2327748 R TIGRFAM: C4-dicarboxylate transporter/malic acid transporter; PFAM: C4-dicarboxylate transporter/malic acid transport protein; KEGG: sfv:SFV_1777 potassium-tellurite ethidium and proflavin transporter; potassium-tellurite ethidium and proflavin transporter complement(2326756..2327748) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157317 YP_003036428.1 CDS ECBD_2212 NC_012947.1 2327872 2328852 D hypothetical protein 2327872..2328852 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157318 YP_003036429.1 CDS ECBD_2213 NC_012947.1 2328844 2329383 R PFAM: GCN5-related N-acetyltransferase; KEGG: elf:LF82_1889 ribosomal-protein-serine acetyltransferase; ribosomal-protein-L7/L12-serine acetyltransferase complement(2328844..2329383) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157319 YP_003036430.1 CDS ECBD_2214 NC_012947.1 2329446 2329670 R PFAM: protein of unknown function DUF465; KEGG: sfv:SFV_1780 hypothetical protein; hypothetical protein complement(2329446..2329670) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157320 YP_003036431.1 CDS mdoD NC_012947.1 2329810 2331429 R involved in the control of the structural glucose backbone of osmoregulated periplasmic glucans; glucan biosynthesis protein D complement(2329810..2331429) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157321 YP_003036432.1 CDS ECBD_2216 NC_012947.1 2331690 2333033 R PFAM: protein of unknown function DUF1338; KEGG: ssn:SSON_1719 hypothetical protein; hypothetical protein complement(2331690..2333033) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157322 YP_003036433.1 CDS ECBD_2217 NC_012947.1 2333250 2334173 D PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: ssn:SSON_1720 transcriptional regulator LysR-type; LysR family transcriptional regulator 2333250..2334173 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157323 YP_003036434.1 CDS ECBD_2218 NC_012947.1 2334211 2335851 R PFAM: chemotaxis sensory transducer; ligand binding Tar domain protein; histidine kinase HAMP region domain protein; SMART: chemotaxis sensory transducer; histidine kinase HAMP region domain protein; ligand binding Tar domain protein; KEGG: sfx:S1485 methyl-accepting chemotaxis protein III, ribose sensor receptor; methyl-accepting chemotaxis sensory transducer complement(2334211..2335851) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157324 YP_003036435.1 CDS ECBD_2219 NC_012947.1 2336192 2336359 D KEGG: ssn:SSON_1722 hypothetical protein; hypothetical protein 2336192..2336359 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157325 YP_003036436.1 CDS ECBD_2220 NC_012947.1 2336471 2336644 R KEGG: ssn:SSON_1723 hypothetical protein; hypothetical protein complement(2336471..2336644) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157326 YP_003036437.1 CDS ECBD_2221 NC_012947.1 2336889 2337419 R B-type di-heme cytochrome with a major alpha-absorption peak at 561 nm and a minor peak at 555 nm; cytochrome b561 complement(2336889..2337419) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157327 YP_003036438.1 CDS ECBD_2222 NC_012947.1 2337518 2338021 R PFAM: IS1 transposase; KEGG: sty:HCM1.136a IS1 transposase; IS1 transposase complement(2337518..2338021) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157328 YP_003036439.1 CDS ECBD_2223 NC_012947.1 2337940 2338215 R PFAM: Insertion element protein; KEGG: dps:DP0861 insertion element iso-IS1d protein (InsA); Insertion element protein complement(2337940..2338215) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157329 YP_003036440.1 CDS ECBD_2224 NC_012947.1 2338384 2339385 D KEGG: ssn:SSON_1725 glyceraldehyde-3-phosphate dehydrogenase; TIGRFAM: glyceraldehyde-3-phosphate dehydrogenase; PFAM: glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase 2338384..2339385 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157330 YP_003036441.1 CDS ECBD_2225 NC_012947.1 2339427 2340866 R NAD-linked; aldehyde dehydrogenase A complement(2339427..2340866) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157331 YP_003036442.1 CDS ECBD_2226 NC_012947.1 2341063 2341863 R PFAM: protein of unknown function DUF218; hypothetical protein complement(2341063..2341863) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157332 YP_003036443.1 CDS ECBD_2227 NC_012947.1 2342135 2346037 R involved in the post-transcriptional processing of the daa operon mRNA, which encodes proteins involved in fimbrial biogenesis of an enteropathogenic E. coli strain; ATP-dependent RNA helicase HrpA complement(2342135..2346037) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157333 YP_003036444.1 CDS ECBD_2228 NC_012947.1 2346238 2346843 D FMN-dependent; requires NADH; catalyzes the cleavage of azo bond in aromatic azo compounds; azoreductase 2346238..2346843 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157334 YP_003036445.1 CDS ECBD_2229 NC_012947.1 2346894 2348210 R PFAM: Dual specificity protein phosphatase; phosphoesterase PA-phosphatase related; SMART: Dual specificity protein phosphatase; protein-tyrosine phosphatase, catalytic; KEGG: sbc:SbBS512_E1631 PAP2 family protein; dual specificity phosphatase complement(2346894..2348210) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157335 YP_003036446.1 CDS ECBD_2230 NC_012947.1 2348200 2349957 R KEGG: sdy:SDY_1779 hypothetical protein; hypothetical protein complement(2348200..2349957) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157336 YP_003036447.1 CDS ECBD_2231 NC_012947.1 2349973 2350869 R PFAM: phosphatidate cytidylyltransferase; KEGG: elf:LF82_3553 uncharacterized protein YnbB; phosphatidate cytidylyltransferase complement(2349973..2350869) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157337 YP_003036448.1 CDS ECBD_2232 NC_012947.1 2350869 2351474 R PFAM: CDP-alcohol phosphatidyltransferase; KEGG: efe:EFER_1593 conserved hypothetical protein; inner membrane protein; CDP-alcohol phosphatidyltransferase complement(2350869..2351474) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157338 YP_003036449.1 CDS ECBD_2233 NC_012947.1 2351645 2353951 R hypothetical protein complement(2351645..2353951) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157339 YP_003036450.1 CDS ECBD_2235 NC_012947.1 2354566 2355432 R PFAM: Integrase catalytic region; KEGG: seh:SeHA_C4704 ISEc16, orfB; Integrase catalytic region complement(2354566..2355432) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157341 YP_003036451.1 CDS ECBD_2236 NC_012947.1 2355429 2355728 R PFAM: transposase IS3/IS911 family protein; transposase IS3/IS911 family protein complement(2355429..2355728) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158744 YP_003036452.1 CDS ECBD_2238 NC_012947.1 2357805 2359304 R PFAM: aldehyde dehydrogenase; KEGG: efe:EFER_1612 phenylacetaldehyde dehydrogenase; Phenylacetaldehyde dehydrogenase complement(2357805..2359304) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159077 YP_003036453.1 CDS ECBD_2239 NC_012947.1 2359540 2360445 D activator of genes involved in phenylacetic acid catabolism; FeaR family transcriptional regulator 2359540..2360445 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159078 YP_003036454.1 CDS ECBD_2240 NC_012947.1 2360617 2360943 R PFAM: conserved hypothetical protein; KEGG: sbo:SBO_1683 hypothetical protein; hypothetical protein complement(2360617..2360943) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159079 YP_003036455.1 CDS ECBD_2241 NC_012947.1 2360951 2361136 R KEGG: ssn:SSON_1742 hypothetical protein; hypothetical protein complement(2360951..2361136) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159080 YP_003036456.1 CDS ECBD_2242 NC_012947.1 2361133 2363772 R KEGG: sbo:SBO_1685 hypothetical protein; hypothetical protein complement(2361133..2363772) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159081 YP_003036457.1 CDS ECBD_2243 NC_012947.1 2363980 2364969 D PFAM: D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding; D-isomer specific 2-hydroxyacid dehydrogenase catalytic region; KEGG: sbc:SbBS512_E1618 D-lactate dehydrogenase; D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding 2363980..2364969 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159082 YP_003036458.1 CDS ECBD_2244 NC_012947.1 2365080 2365502 D PFAM: protein of unknown function DUF306 Meta and HslJ; KEGG: ssn:SSON_1745 heat-inducible protein; heat-inducible protein 2365080..2365502 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159083 YP_003036459.1 CDS ECBD_2245 NC_012947.1 2365499 2365765 R KEGG: ssn:SSON_1746 cytoplasmic protein; cytoplasmic protein complement(2365499..2365765) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159084 YP_003036460.1 CDS ECBD_2246 NC_012947.1 2366039 2369563 D TIGRFAM: pyruvate ferredoxin/flavodoxin oxidoreductase; PFAM: pyruvate flavodoxin/ferredoxin oxidoreductase domain protein; pyruvate ferredoxin/flavodoxin oxidoreductase; 4Fe-4S ferredoxin iron-sulfur binding domain protein; pyruvate ferredoxin/flavodoxin oxidoreductase 2366039..2369563 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159085 YP_003036461.1 CDS ECBD_2247 NC_012947.1 2369930 2371063 D PFAM: porin Gram-negative type; KEGG: sbc:SbBS512_E1614 outer membrane protein N; porin Gram-negative type 2369930..2371063 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159086 YP_003036462.1 CDS ECBD_2248 NC_012947.1 2371204 2371638 D PFAM: UspA domain protein; KEGG: ssn:SSON_1749 filament protein; UspA domain protein 2371204..2371638 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159087 YP_003036463.1 CDS ECBD_2249 NC_012947.1 2372599 2372832 D KEGG: elf:LF82_2833 uncharacterized protein YdfK; hypothetical protein 2372599..2372832 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159088 YP_003036464.1 CDS ECBD_2250 NC_012947.1 2373149 2373739 D PFAM: Resolvase domain; Resolvase helix-turn-helix domain protein; KEGG: elf:LF82_1666 DNA-invertase from lambdoid prophage Rac; resolvase 2373149..2373739 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159089 YP_003036465.1 CDS ECBD_2251 NC_012947.1 2373967 2374260 R KEGG: elf:LF82_p183 hypothetical protein; hypothetical protein complement(2373967..2374260) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159090 YP_003036466.1 CDS ECBD_2252 NC_012947.1 2374273 2374551 R KEGG: elf:LF82_p182 hypothetical protein; hypothetical protein complement(2374273..2374551) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159091 YP_003036467.1 CDS ECBD_2253 NC_012947.1 2374548 2375285 R KEGG: elf:LF82_p261 hypothetical protein; hypothetical protein complement(2374548..2375285) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159092 YP_003036468.1 CDS ECBD_2254 NC_012947.1 2375233 2375628 R PFAM: ParB domain protein nuclease; SMART: ParB domain protein nuclease; KEGG: ypi:YpsIP31758_2231 ParB family protein; ParB domain protein nuclease complement(2375233..2375628) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159093 YP_003036469.1 CDS ECBD_2255 NC_012947.1 2375766 2377223 R TIGRFAM: potassium uptake protein, TrkH family; PFAM: cation transporter; KEGG: efe:EFER_1950 trk system potassium uptake protein; potassium uptake protein, TrkH family complement(2375766..2377223) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159094 YP_003036470.1 CDS ECBD_2256 NC_012947.1 2377361 2377825 R PFAM: lysis protein; KEGG: elf:LF82_2080 Rz endopeptidase from lambdoid prophage DLP12; lysis protein complement(2377361..2377825) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159095 YP_003036471.1 CDS ECBD_2257 NC_012947.1 2377822 2378355 R PFAM: glycoside hydrolase family 24; KEGG: sbc:SbBS512_E1450 phage lysozyme; Lysozyme complement(2377822..2378355) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159096 YP_003036472.1 CDS ECBD_2258 NC_012947.1 2378461 2378733 D KEGG: sbc:SbBS512_E0946 hypothetical protein; hypothetical protein 2378461..2378733 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159097 YP_003036473.1 CDS ECBD_2259 NC_012947.1 2378699 2379043 R PFAM: protein of unknown function DUF1327; KEGG: sbc:SbBS512_E0945 hypothetical protein; hypothetical protein complement(2378699..2379043) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159098 YP_003036474.1 CDS ECBD_2260 NC_012947.1 2379048 2379260 R PFAM: Lysis S family protein; KEGG: elf:LF82_0578 lysis protein S homolog from lambdoid prophage DLP12; Lysis S family protein complement(2379048..2379260) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159099 YP_003036475.1 CDS ECBD_2261 NC_012947.1 2379259 2379393 D hypothetical protein 2379259..2379393 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159100 YP_003036476.1 CDS ECBD_2262 NC_012947.1 2379404 2379559 D PFAM: protein of unknown function DUF1391; KEGG: sbc:SbBS512_E0694 hypothetical protein; hypothetical protein 2379404..2379559 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159101 YP_003036477.1 CDS ECBD_2263 NC_012947.1 2379556 2380044 R KEGG: set:SEN1382 phage membrane protein; hypothetical protein complement(2379556..2380044) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159102 YP_003036478.1 CDS kil NC_012947.1 2380486 2380707 D Rac prophage Kil protein; Kil overexpression leads to morphological defects and inhibits cell division; FtsZ inhibitor protein 2380486..2380707 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159103 YP_003036479.1 CDS ECBD_2265 NC_012947.1 2380707 2380877 D KEGG: sty:STY2071 bacteriophage protein; bacteriophage protein 2380707..2380877 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159104 YP_003036480.1 CDS ECBD_2266 NC_012947.1 2380952 2381227 D Rac prophage hypothetical protein; hypothetical protein 2380952..2381227 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159105 YP_003036481.1 CDS recT NC_012947.1 2382421 2383230 D TIGRFAM: RecT protein; PFAM: RecT protein; KEGG: spq:SPAB_02270 recombination and repair protein RecT; recombination and repair protein RecT 2382421..2383230 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159107 YP_003036482.1 CDS ECBD_2269 NC_012947.1 2383474 2383683 D Rac prophage; hypothetical protein 2383474..2383683 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159108 YP_003036483.1 CDS ECBD_2270 NC_012947.1 2383762 2383977 D PFAM: protein of unknown function DUF1233 excisionase KEGG: sfl:SF1369 hypothetical protein; hypothetical protein 2383762..2383977 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159109 YP_003036484.1 CDS ECBD_2271 NC_012947.1 2383979 2385214 D PFAM: integrase; KEGG: sbo:SBO_1716 transposase; integrase 2383979..2385214 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159110 YP_003036485.1 CDS ECBD_2272 NC_012947.1 2385266 2386201 D TtcA; YdaO; catalyzes the thiolation of cytosine 32 in specific tRNAs; C32 tRNA thiolase 2385266..2386201 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159111 YP_003036486.1 CDS ECBD_2273 NC_012947.1 2386330 2387703 R exhibits an RNA-dependent ATPase activity, specifically stimulated by bacterial 23S rRNA; ATP-dependent RNA helicase DbpA complement(2386330..2387703) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159112 YP_003036487.1 CDS ECBD_2274 NC_012947.1 2387733 2387906 R KEGG: efe:EFER_1627 hypothetical protein; hypothetical protein complement(2387733..2387906) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159113 YP_003036488.1 CDS zntB NC_012947.1 2388181 2389164 R PFAM: Mg2 transporter protein CorA family protein; KEGG: sdy:SDY_1424 zinc transporter; zinc transporter complement(2388181..2389164) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159114 YP_003036489.1 CDS ECBD_2276 NC_012947.1 2389419 2390651 D KEGG: sbo:SBO_1720 hypothetical protein; TIGRFAM: diguanylate cyclase; PAS sensor protein; PFAM: GGDEF domain containing protein; hypothetical protein; SMART: GGDEF domain containing protein; PAS domain containing protein; diguanylate cyclase with PAS/PAC sensor 2389419..2390651 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159115 YP_003036490.1 CDS ECBD_2277 NC_012947.1 2390672 2391235 R PFAM: Smr protein/MutS2; SMART: Smr protein/MutS2; KEGG: elf:LF82_2783 uncharacterized protein ydal; Smr protein/MutS2 complement(2390672..2391235) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159116 YP_003036491.1 CDS ECBD_2278 NC_012947.1 2391565 2392473 R PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: ssn:SSON_1792 DNA-binding transcriptional regulator; DNA-binding transcriptional regulator complement(2391565..2392473) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159117 YP_003036492.1 CDS ECBD_2279 NC_012947.1 2392649 2393959 D TIGRFAM: amidohydrolase; PFAM: peptidase M20; peptidase dimerisation domain protein; KEGG: ssn:SSON_1793 aminohydrolase; amidohydrolase 2392649..2393959 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159118 YP_003036493.1 CDS ECBD_2280 NC_012947.1 2393959 2395404 D TIGRFAM: amidohydrolase; PFAM: peptidase M20; peptidase dimerisation domain protein; KEGG: ssn:SSON_1794 hypothetical protein; amidohydrolase 2393959..2395404 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159119 YP_003036494.1 CDS abgT NC_012947.1 2395441 2396967 D TIGRFAM: AbgT transporter family; PFAM: AbgT transporter; aminobenzoyl-glutamate transporter 2395441..2396967 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159120 YP_003036495.1 CDS ECBD_2282 NC_012947.1 2396978 2397493 D KEGG: ssn:SSON_1798 O-6-alkylguanine-DNA:cysteine-protein methyltransferase; TIGRFAM: methylated-DNA/protein-cysteine methyltransferase; PFAM: methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding; methylguanine DNA methyltransferase ribonuclease domain protein; O-6-alkylguanine-DNA:cysteine-protein methyltransferase 2396978..2397493 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159121 YP_003036496.1 CDS ECBD_2284 NC_012947.1 2398592 2399542 D with UspC and UspD is involved in resistance to UV irradiation; universal stress protein UspE 2398592..2399542 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159122 YP_003036497.1 CDS ECBD_2285 NC_012947.1 2399592 2399849 R KEGG: ssn:SSON_1801 hypothetical protein; hypothetical protein complement(2399592..2399849) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159123 YP_003036498.1 CDS ECBD_2286 NC_012947.1 2400093 2401124 D PFAM: MscS Mechanosensitive ion channel; KEGG: sbc:SbBS512_E1570 transporter small conductance mechanosensitive ion channel family; MscS Mechanosensitive ion channel 2400093..2401124 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159124 YP_003036499.1 CDS ECBD_2287 NC_012947.1 2401175 2402788 R PFAM: extracellular solute-binding protein family 5; KEGG: ssn:SSON_1804 transport periplasmic protein; extracellular solute-binding protein family 5 complement(2401175..2402788) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159125 YP_003036500.1 CDS ECBD_2288 NC_012947.1 2403125 2404024 R PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: sbo:SBO_1734 transcriptional regulator LysR-type; LysR family transcriptional regulator complement(2403125..2404024) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159126 YP_003036501.1 CDS ECBD_2289 NC_012947.1 2404162 2405082 D KEGG: ssn:SSON_1806 hypothetical protein; hypothetical protein 2404162..2405082 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159127 YP_003036502.1 CDS ECBD_2290 NC_012947.1 2405153 2405335 D KEGG: ssn:SSON_1810 hypothetical protein; hypothetical protein 2405153..2405335 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159128 YP_003036503.1 CDS ECBD_2291 NC_012947.1 2405417 2406145 D PFAM: peptidase M14 carboxypeptidase A; KEGG: ssn:SSON_1812 murein peptide amidase A; murein peptide amidase A 2405417..2406145 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159129 YP_003036504.1 CDS ECBD_2292 NC_012947.1 2406120 2407085 R PFAM: mandelate racemase/muconate lactonizing protein; KEGG: sfv:SFV_1341 muconate cycloisomerase I; mandelate racemase/muconate lactonizing protein complement(2406120..2407085) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159130 YP_003036505.1 CDS tpx NC_012947.1 2407204 2407710 D antioxidant activity; thioredoxin-dependent thiol peroxidase; forms homodimers in solution; shows substrate specificity to alkyl hydroperoxides; periplasmic protein; thiol peroxidase 2407204..2407710 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159131 YP_003036506.1 CDS ECBD_2294 NC_012947.1 2407754 2409295 R regulates genes involved in the biosynthesis and transport of aromatic amino acids; DNA-binding transcriptional regulator TyrR complement(2407754..2409295) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159132 YP_003036507.1 CDS ECBD_2295 NC_012947.1 2409443 2410504 R PFAM: conserved hypothetical protein; KEGG: elf:LF82_2767 UPF0283 membrane protein YcjF; hypothetical protein complement(2409443..2410504) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159133 YP_003036508.1 CDS ECBD_2296 NC_012947.1 2410501 2411898 R PFAM: protein of unknown function DUF463 YcjX family protein; KEGG: sbc:SbBS512_E1554 hypothetical protein; hypothetical protein complement(2410501..2411898) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159134 YP_003036509.1 CDS ECBD_2297 NC_012947.1 2412054 2413052 D PFAM: regulatory protein LacI; periplasmic binding protein/LacI transcriptional regulator; SMART: regulatory protein LacI; KEGG: sbc:SbBS512_E1553 LacI family transcriptional regulator; LacI family transcriptional regulator 2412054..2413052 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159135 YP_003036510.1 CDS ECBD_2298 NC_012947.1 2413163 2414068 R hypothetical protein complement(2413163..2414068) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159136 YP_003036511.1 CDS ECBD_2299 NC_012947.1 2414113 2415195 R PFAM: ABC transporter; TOBE domain protein; transport-associated OB domain protein; SMART: ATPase AAA; ABC transporter complement(2414113..2415195) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159137 YP_003036512.1 CDS ECBD_2300 NC_012947.1 2415209 2415868 R TIGRFAM: beta-phosphoglucomutase; beta-phosphoglucomutase family hydrolase; HAD-superfamily hydrolase, subfamily IA, variant 3; PFAM: haloacid dehalogenase; beta-phosphoglucomutase complement(2415209..2415868) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159138 YP_003036513.1 CDS ECBD_2301 NC_012947.1 2415865 2418132 R PFAM: glycoside hydrolase family 65 central catalytic; glycoside hydrolase family 65 domain protein; KEGG: ssn:SSON_1824 hypothetical protein; glycoside hydrolase family 65 central catalytic complement(2415865..2418132) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159139 YP_003036514.1 CDS ECBD_2302 NC_012947.1 2418129 2419184 R PFAM: oxidoreductase domain protein; oxidoreductase domain; KEGG: elf:LF82_2775 oxidoreductase YcjS; oxidoreductase domain protein complement(2418129..2419184) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159140 YP_003036515.1 CDS ECBD_2303 NC_012947.1 2419194 2419982 R PFAM: xylose isomerase; KEGG: elf:LF82_2774 uncharacterized protein YcjR; xylose isomerase complement(2419194..2419982) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159141 YP_003036516.1 CDS ECBD_2304 NC_012947.1 2420001 2421053 R PFAM: alcohol dehydrogenase zinc-binding domain protein; KEGG: sfv:SFV_1329 oxidoreductase; alcohol dehydrogenase zinc-binding domain protein complement(2420001..2421053) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159142 YP_003036517.1 CDS ECBD_2305 NC_012947.1 2421084 2421926 R PFAM: binding-protein-dependent transporters inner membrane component; KEGG: sfv:SFV_1328 transporter permease protein; binding-protein-dependent transporters inner membrane component complement(2421084..2421926) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159143 YP_003036518.1 CDS ECBD_2306 NC_012947.1 2421913 2422794 R PFAM: binding-protein-dependent transporters inner membrane component; KEGG: ssn:SSON_1829 binding-protein dependent transporter; binding-protein-dependent transporters inner membrane component complement(2421913..2422794) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159144 YP_003036519.1 CDS ECBD_2307 NC_012947.1 2422815 2424107 R PFAM: extracellular solute-binding protein family 1; extracellular solute-binding protein family 1 complement(2422815..2424107) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159145 YP_003036520.1 CDS ECBD_2308 NC_012947.1 2424121 2425800 R PFAM: alpha amylase catalytic region; SMART: alpha amylase catalytic sub domain; alpha amylase catalytic region complement(2424121..2425800) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159146 YP_003036521.1 CDS ECBD_2309 NC_012947.1 2426013 2426327 R rhodanese; catalyzes the formation of thiocyanate from thiosulfate and hydrogen cyanide; thiosulfate:cyanide sulfurtransferase complement(2426013..2426327) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159147 YP_003036522.1 CDS ECBD_2310 NC_012947.1 2426402 2426623 R TIGRFAM: phage shock protein PspD; KEGG: ssn:SSON_1833 peripheral inner membrane phage-shock protein; peripheral inner membrane phage-shock protein complement(2426402..2426623) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159148 YP_003036523.1 CDS ECBD_2311 NC_012947.1 2426632 2426991 R with PsbB forms toxin/antitoxin pair; activates the psp operon in response to phage infection, exposure to ethanol or osmotic shock; DNA-binding transcriptional activator PspC complement(2426632..2426991) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159149 YP_003036524.1 CDS pspB NC_012947.1 2426991 2427215 R acts together with PspC to induce psp operon during infection with phage, exposure to ethanol or osmotic shock; forms a complex with PspA and C; PspC is required for PspAB binding; phage shock protein B complement(2426991..2427215) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159150 YP_003036525.1 CDS ECBD_2313 NC_012947.1 2427269 2427937 R involved in maintaining membrane potential under membrane stress conditions; also acts as a negative transcriptional regulator of the phage shock protein (psp) operon(pspABCDE) by regulating the transcriptional activator PspF; phage shock protein PspA complement(2427269..2427937) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159151 YP_003036526.1 CDS pspF NC_012947.1 2428104 2429081 D transcriptional activator for pspABCE which are induced in response to phage proteins, membrane altering stresses, and impaired proton expor; phage shock protein operon transcriptional activator 2428104..2429081 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159152 YP_003036527.1 CDS ECBD_2315 NC_012947.1 2429201 2430466 R putrescine-inducible GABA aminotransferase; catalyzes the formation of glutamate and succinate semialdehyde from 4-aminobutyrate and 2-oxoglutarate; 4-aminobutyrate transaminase complement(2429201..2430466) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159153 YP_003036528.1 CDS ECBD_2316 NC_012947.1 2430504 2431784 R PFAM: FAD dependent oxidoreductase; FAD dependent oxidoreductase complement(2430504..2431784) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159154 YP_003036529.1 CDS ECBD_2317 NC_012947.1 2431786 2433273 R catalyzes the formation of gamma-glutamyl-gamma-aminobutyrate from gamma-glutamyl-gamma-aminobutyraldehyde; involved in putrescine degradation; gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase complement(2431786..2433273) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159155 YP_003036530.1 CDS ECBD_2318 NC_012947.1 2433548 2434105 R regulates genes involved in putrescine degradation; DNA-binding transcriptional repressor PuuR complement(2433548..2434105) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159156 YP_003036531.1 CDS puuD NC_012947.1 2434132 2434896 R catalyzes the degradation of gamma-glutamyl-gamma-aminobutyrate into glutamate and gamma-aminobutyrate; gamma-glutamyl-gamma-aminobutyrate hydrolase complement(2434132..2434896) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158074 YP_003036532.1 CDS ECBD_2320 NC_012947.1 2435108 2436526 D PFAM: glutamine synthetase; glutamate--putrescine ligase 2435108..2436526 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158075 YP_003036533.1 CDS ECBD_2321 NC_012947.1 2436829 2438214 D PFAM: amino acid permease-associated region; KEGG: ssn:SSON_1844 amino acid/amine transporter; amino acid permease-associated region 2436829..2438214 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158076 YP_003036534.1 CDS ECBD_2322 NC_012947.1 2438348 2438593 D KEGG: sdy:SDY_1637 hypothetical protein; hypothetical protein 2438348..2438593 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158077 YP_003036535.1 CDS ECBD_2323 NC_012947.1 2438905 2440548 D PFAM: extracellular solute-binding protein family 5; KEGG: ssn:SSON_1846 peptide transport periplasmic protein-like protein; extracellular solute-binding protein family 5 2438905..2440548 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158078 YP_003036536.1 CDS ECBD_2324 NC_012947.1 2440545 2441510 D PFAM: binding-protein-dependent transporters inner membrane component; KEGG: sbc:SbBS512_E1529 peptide ABC transporter permease protein SapB; binding-protein-dependent transporters inner membrane component 2440545..2441510 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158079 YP_003036537.1 CDS ECBD_2325 NC_012947.1 2441497 2442387 D PFAM: binding-protein-dependent transporters inner membrane component; KEGG: sbc:SbBS512_E1528 peptide ABC transporter permease protein SapC; binding-protein-dependent transporters inner membrane component 2441497..2442387 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158080 YP_003036538.1 CDS ECBD_2326 NC_012947.1 2442387 2443379 D KEGG: sbc:SbBS512_E1527 peptide ABC transporter ATP-binding protein SapD; TIGRFAM: oligopeptide/dipeptide ABC transporter ATPase; PFAM: ABC transporter; oligopeptide/dipeptide ABC transporter domain protein; SMART: ATPase AAA; peptide ABC transporter ATPase 2442387..2443379 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158081 YP_003036539.1 CDS ECBD_2327 NC_012947.1 2443381 2444187 D PFAM: ABC transporter; SMART: ATPase AAA; KEGG: sbc:SbBS512_E1526 peptide ABC transporter ATP-binding protein SapF; ABC transporter 2443381..2444187 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158082 YP_003036540.1 CDS ECBD_2328 NC_012947.1 2444255 2444608 D PFAM: protein of unknown function DUF559; KEGG: sbo:SBO_1773 hypothetical protein; hypothetical protein 2444255..2444608 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158083 YP_003036541.1 CDS ECBD_2329 NC_012947.1 2444976 2445764 D Catalyzes a key regulatory step in fatty acid biosynthesis; enoyl-(acyl carrier protein) reductase 2444976..2445764 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158084 YP_003036542.1 CDS ECBD_2330 NC_012947.1 2445908 2447035 D KEGG: sbc:SbBS512_E1521 hypothetical protein; hypothetical protein 2445908..2447035 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158085 YP_003036543.1 CDS ECBD_2331 NC_012947.1 2447103 2449037 D Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3' to 5' direction; exoribonuclease II 2447103..2449037 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158086 YP_003036544.1 CDS ECBD_2332 NC_012947.1 2449114 2449218 R hypothetical protein complement(2449114..2449218) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158087 YP_003036545.1 CDS ECBD_2333 NC_012947.1 2449272 2451257 D c-di-GMP phosphodiesterase; probably degrades signalling molecule c-di-GMP; RNase II stability modulator 2449272..2451257 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158088 YP_003036546.1 CDS ECBD_2334 NC_012947.1 2451404 2451577 D KEGG: sdy:SDY_1362 hypothetical protein; hypothetical protein 2451404..2451577 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158089 YP_003036547.1 CDS ECBD_2335 NC_012947.1 2451667 2452416 D PFAM: regulatory protein DeoR; Helix-turn-helix type 11 domain protein; SMART: regulatory protein DeoR; KEGG: ssn:SSON_1856 DeoR-type transcriptional regulator; DeoR family transcriptional regulator 2451667..2452416 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158090 YP_003036548.1 CDS ECBD_2336 NC_012947.1 2452685 2452903 D KEGG: ssn:SSON_1857 lipoprotein; lipoprotein 2452685..2452903 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158091 YP_003036549.1 CDS ECBD_2337 NC_012947.1 2453029 2453355 R involved in start site selection during the initiation of translation; translation initiation factor Sui1 complement(2453029..2453355) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158092 YP_003036550.1 CDS ECBD_2338 NC_012947.1 2453355 2454152 R type 1 subfamily; involved in last step of pyrimidine biosynthesis; converts orotidine 5'-phosphate to UMP and carbon dioxide; OMP decarboxylase; OMPDCase; OMPdecase; orotidine 5'-phosphate decarboxylase complement(2453355..2454152) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158093 YP_003036551.1 CDS ECBD_2339 NC_012947.1 2454285 2455454 R SMART: hypothetical protein; KEGG: ssn:SSON_1860 hypothetical protein; hypothetical protein complement(2454285..2455454) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158094 YP_003036552.1 CDS ECBD_2340 NC_012947.1 2455461 2455769 R PFAM: protein of unknown function DUF1049; KEGG: sfv:SFV_1292 hypothetical protein; hypothetical protein complement(2455461..2455769) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158095 YP_003036553.1 CDS ECBD_2341 NC_012947.1 2455918 2456682 R KEGG: sdy:SDY_1354 phosphatidylglycerophosphatase B; PFAM: phosphoesterase PA-phosphatase related; SMART: phosphoesterase PA-phosphatase related; phosphatidylglycerophosphatase B complement(2455918..2456682) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158096 YP_003036554.1 CDS ribA NC_012947.1 2456852 2457442 D catalyzes the conversion of GTP to formate and 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine and diphosphate; GTP cyclohydrolase II 2456852..2457442 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158097 YP_003036555.1 CDS ECBD_2343 NC_012947.1 2457506 2460181 R Catalyzes the conversion of citrate to isocitrate; aconitate hydratase complement(2457506..2460181) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158098 YP_003036556.1 CDS ECBD_2344 NC_012947.1 2460355 2460480 D hypothetical protein 2460355..2460480 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158099 YP_003036557.1 CDS ECBD_2345 NC_012947.1 2460554 2460721 R KEGG: sbc:SbBS512_E1503 hypothetical protein; hypothetical protein complement(2460554..2460721) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158100 YP_003036558.1 CDS ECBD_2346 NC_012947.1 2460724 2460852 R KEGG: sdy:SDY_1348 hypothetical protein; hypothetical protein complement(2460724..2460852) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158101 YP_003036559.1 CDS cysB NC_012947.1 2461183 2462157 R LysR-type transcriptional regulator; contains helix-turn-helix (HTH) motif; in Escherichia coli this protein regulates cysteine biosynthesis by controlling expression of the cys regulon; autoregulates expression; crystal structure of Klebsiella aerogenes showed tetramer formation; transcriptional regulator CysB complement(2461183..2462157) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158102 YP_003036560.1 CDS ECBD_2348 NC_012947.1 2462367 2464964 R catalyzes the ATP-dependent breakage of single-stranded DNA followed by passage and rejoining, maintains net negative superhelicity; DNA topoisomerase I complement(2462367..2464964) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158103 YP_003036561.1 CDS ECBD_2349 NC_012947.1 2465344 2465595 D KEGG: hypothetical protein; hypothetical protein 2465344..2465595 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158104 YP_003036562.1 CDS ECBD_2350 NC_012947.1 2465631 2466680 R SohB; periplasmic protein; member of the peptidase S49 family; periplasmic protease complement(2465631..2466680) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158105 YP_003036563.1 CDS ECBD_2351 NC_012947.1 2466900 2467658 D PFAM: short-chain dehydrogenase/reductase SDR; KR domain protein; KEGG: ssn:SSON_1872 short chain dehydrogenase; short chain dehydrogenase 2466900..2467658 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158106 YP_003036564.1 CDS ECBD_2352 NC_012947.1 2467655 2468245 D catalyzes the formation of adenosylcob(III)yrinic acid a,c-diamide from cob(I)yrinic acid a,c-diamide; cob(I)yrinic acid a,c-diamide adenosyltransferase 2467655..2468245 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158107 YP_003036565.1 CDS ECBD_2353 NC_012947.1 2468285 2469160 R catalyzes the synthesis of pseudouridine from uracil-2605 in 23S ribosomal RNA; 23S rRNA pseudouridylate synthase B complement(2468285..2469160) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158108 YP_003036566.1 CDS ECBD_2355 NC_012947.1 2470911 2471414 R PFAM: IS1 transposase; KEGG: sty:HCM1.136a IS1 transposase; IS1 transposase complement(2470911..2471414) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158110 YP_003036567.1 CDS ECBD_2356 NC_012947.1 2472075 2472695 R TIGRFAM: Sua5/YciO/YrdC/YwlC family protein; PFAM: SUA5/yciO/yrdC domain; KEGG: sbc:SbBS512_E1491 SUA5/YciO/YrdC/YwlC family protein; hypothetical protein complement(2472075..2472695) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158111 YP_003036568.1 CDS ECBD_2357 NC_012947.1 2472692 2473573 R PFAM: PHP domain protein; SMART: phosphoesterase PHP domain protein; KEGG: elf:LF82_2314 protein TrpH; PHP domain protein complement(2472692..2473573) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158112 YP_003036569.1 CDS ECBD_2358 NC_012947.1 2473847 2475409 D with component II, the glutamine amidotransferase, catalyzes the formation of anthranilate from chorismate and glutamine; anthranilate synthase component I 2473847..2475409 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158113 YP_003036570.1 CDS ECBD_2359 NC_012947.1 2475409 2477004 D bifunctional anthranilate synthase II/anthranilate phosphoribosyltransferase; TrpD; forms a heterotetramer with Trp E and the complex catalyzes the formation of anthranilate from chorismate and glutamine; also catalyzes the formation of N-(5-phospho-D-ribosyl)-anthranilate from athranilate and 5-phospho-alpha-D-ribose 1-diphosphate; functions in tryptophan biosynthesis; bifunctional glutamine amidotransferase/anthranilate phosphoribosyltransferase 2475409..2477004 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158114 YP_003036571.1 CDS ECBD_2360 NC_012947.1 2477008 2478366 D monomeric bifunctional protein; functions in tryptophan biosynthesis pathway; phosphoribosylanthranilate is rearranged to carboxyphenylaminodeoxyribulosephosphate which is then closed to form indole-3-glycerol phosphate; bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase 2477008..2478366 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158115 YP_003036572.1 CDS ECBD_2361 NC_012947.1 2478378 2479571 D catalyzes the formation of L-tryptophan from L-serine and 1-(indol-3-yl)glycerol 3-phosphate; tryptophan synthase subunit beta 2478378..2479571 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158116 YP_003036573.1 CDS trpA NC_012947.1 2479571 2480377 D catalyzes the formation of indole and glyceraldehyde 3-phosphate from indoleglycerol phosphate in tryptophan biosynthesis; tryptophan synthase subunit alpha 2479571..2480377 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158117 YP_003036574.1 CDS ECBD_2363 NC_012947.1 2480758 2480937 D KEGG: sfv:SFV_1273 hypothetical protein; hypothetical protein 2480758..2480937 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158118 YP_003036575.1 CDS ECBD_2364 NC_012947.1 2481023 2481523 D PFAM: protein of unknown function DUF892; KEGG: elf:LF82_2752 protein YciF; hypothetical protein 2481023..2481523 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158119 YP_003036576.1 CDS ECBD_2365 NC_012947.1 2481569 2482075 D PFAM: protein of unknown function DUF892; hypothetical protein 2481569..2482075 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158120 YP_003036577.1 CDS ECBD_2366 NC_012947.1 2482135 2482773 R receptor for colicin S4; outer membrane protein W complement(2482135..2482773) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158121 YP_003036578.1 CDS ECBD_2367 NC_012947.1 2483130 2483873 D PFAM: protein of unknown function UPF0259; KEGG: ssn:SSON_1911 hypothetical protein; hypothetical protein 2483130..2483873 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158122 YP_003036579.1 CDS ECBD_2368 NC_012947.1 2483903 2484442 D Involved in cell division; probably involved in intracellular septation; intracellular septation protein A 2483903..2484442 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158123 YP_003036580.1 CDS ECBD_2369 NC_012947.1 2484547 2484945 D YciA; hydrolase that catalyzes the hydrolysis of the thioester bond in palmitoyl-CoA and malonyl-CoA; member of the the acyl coenzyme A hydrolase family of proteins; acyl-CoA thioester hydrolase 2484547..2484945 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158124 YP_003036581.1 CDS ECBD_2370 NC_012947.1 2484985 2485719 R membrane spanning protein in TonB-ExbB-ExbD complex; transduces proton motive force of the cytoplasmic membrane to outer membrane transporters; involved in the transport of ron-siderophore complexes, vitamin B12 and colicins; transporter TonB complement(2484985..2485719) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158125 YP_003036582.1 CDS ECBD_2371 NC_012947.1 2485928 2486224 D unknown function; YciI from Haemophilus influenzae presents crystal structure similarity to a muconolactone isomerase, but does not seem to catalyze any of the predicted reactions based on sequence and structure similarity; YciI-like protein 2485928..2486224 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158126 YP_003036583.1 CDS ECBD_2372 NC_012947.1 2486524 2487777 D PFAM: TrkA-N domain protein; ion transport 2 domain protein; KEGG: elf:LF82_1141 potassium channel protein; voltage-gated potassium channel 2486524..2487777 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158127 YP_003036584.1 CDS cls NC_012947.1 2488148 2489608 D catalyzes the transfer of a phosphatidyl group to phosphodidylglycerol to form cardiolipin (diphosphatidylglycerol); cardiolipin synthetase 2488148..2489608 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158128 YP_003036585.1 CDS ECBD_2374 NC_012947.1 2489643 2489972 D highly acidic protein; the structure of the Haemophilus influenzae protein shows similarity to uracil DNA glycosylase inhibitor (Ugi); binds heat-unstable protein (HU-alpha) which is a histone-like protein; dsDNA-mimic protein 2489643..2489972 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158129 YP_003036586.1 CDS ECBD_2375 NC_012947.1 2490025 2491029 R KEGG: sfv:SFV_1259 ATP-binding protein of oligopeptide ABC transporter; TIGRFAM: oligopeptide/dipeptide ABC transporter ATPase subunit; PFAM: ABC transporter; oligopeptide/dipeptide ABC transporter domain protein; SMART: ATPase AAA; peptide ABC transporter ATPase complement(2490025..2491029) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158130 YP_003036587.1 CDS oppD NC_012947.1 2491026 2492039 R KEGG: sbc:SbBS512_E1415 oligopeptide ABC transporter ATP-binding protein OppD; TIGRFAM: oligopeptide/dipeptide ABC transporter ATPase; PFAM: ABC transporter; oligopeptide/dipeptide ABC transporter domain protein; SMART: ATPase AAA; oligopeptide transporter ATP-binding component complement(2491026..2492039) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158131 YP_003036588.1 CDS ECBD_2377 NC_012947.1 2492051 2492959 R PFAM: binding-protein-dependent transporters inner membrane component; KEGG: ssn:SSON_1935 oligopeptide transport permease protein-like protein; binding-protein-dependent transporters inner membrane component complement(2492051..2492959) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158132 YP_003036589.1 CDS oppB NC_012947.1 2492974 2493894 R PFAM: binding-protein-dependent transporters inner membrane component; KEGG: sbo:SBO_1823 oligopeptide transporter permease; oligopeptide transporter permease complement(2492974..2493894) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158133 YP_003036590.1 CDS ECBD_2379 NC_012947.1 2493980 2495656 R PFAM: extracellular solute-binding protein family 5; KEGG: sbo:SBO_1824 oligopeptide transport periplasmic binding protein; extracellular solute-binding protein family 5 complement(2493980..2495656) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158134 YP_003036591.1 CDS ECBD_2380 NC_012947.1 2495895 2496191 D KEGG: elf:LF82_p209 hypothetical protein; hypothetical protein 2495895..2496191 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158135 YP_003036592.1 CDS ECBD_2381 NC_012947.1 2496167 2496322 R hypothetical protein complement(2496167..2496322) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158136 YP_003036593.1 CDS ECBD_2382 NC_012947.1 2496349 2496996 R PFAM: multiple antibiotic resistance (MarC)-related protein; KEGG: ssn:SSON_1938 hypothetical protein; hypothetical protein complement(2496349..2496996) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158137 YP_003036594.1 CDS ECBD_2383 NC_012947.1 2497473 2500148 D PFAM: iron-containing alcohol dehydrogenase; aldehyde dehydrogenase; KEGG: ssn:SSON_1939 CoA-linked acetaldehyde dehydrogenase and iron-dependent alcohol dehydrogenase; pyruvate-formate-lyase deactivase; bifunctional acetaldehyde-CoA/alcohol dehydrogenase 2497473..2500148 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158138 YP_003036595.1 CDS ECBD_2384 NC_012947.1 2500450 2501067 R catalyzes the formation of thymidine 5'-phosphate from thymidine; thymidine kinase complement(2500450..2501067) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158139 YP_003036596.1 CDS ECBD_2385 NC_012947.1 2501671 2502084 D PFAM: histone family protein nucleoid-structuring protein H-NS; SMART: histone family protein nucleoid-structuring protein H-NS; KEGG: ssn:SSON_1942 global DNA-binding transcriptional dual regulator H-NS; global DNA-binding transcriptional dual regulator H-NS 2501671..2502084 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158140 YP_003036597.1 CDS ECBD_2386 NC_012947.1 2502228 2503136 R together with GalF subunit composes the UTP--glucose-1-phosphate uridylyltransferase, an enzyme that catalyzes the formation of UDP-glucose from UTP and alpha-D-glucose 1-phosphate; regulates cellular levels of UDP-glucose; UTP--glucose-1-phosphate uridylyltransferase subunit GalU complement(2502228..2503136) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158141 YP_003036598.1 CDS ECBD_2387 NC_012947.1 2503338 2504351 R PFAM: response regulator receiver; SMART: response regulator receiver; KEGG: sdy:SDY_1287 response regulator of RpoS; response regulator of RpoS complement(2503338..2504351) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158142 YP_003036599.1 CDS ECBD_2388 NC_012947.1 2504443 2505387 R PFAM: patatin; KEGG: ssn:SSON_1945 hypothetical protein; hypothetical protein complement(2504443..2505387) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158143 YP_003036600.1 CDS ECBD_2389 NC_012947.1 2505461 2505919 D PFAM: SEC-C motif domain protein; KEGG: sbc:SbBS512_E1398 sec-C motif domain protein; hypothetical protein 2505461..2505919 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158144 YP_003036601.1 CDS purU NC_012947.1 2505969 2506811 D produces formate from formyl-tetrahydrofolate which is the major source of formate for PurT in de novo purine nucleotide biosynthesis; has a role in one-carbon metabolism; forms a homohexamer; activated by methionine and inhibited by glycine; formyltetrahydrofolate deformylase 2505969..2506811 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158145 YP_003036602.1 CDS ECBD_2391 NC_012947.1 2507711 2508388 R KEGG: sbc:SbBS512_E1395 respiratory nitrate reductase 1, subunit gamma; TIGRFAM: respiratory nitrate reductase, subunit gamma; PFAM: nitrate reductase subunit gamma; respiratory nitrate reductase, subunit gamma complement(2507711..2508388) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158148 YP_003036603.1 CDS ECBD_2392 NC_012947.1 2508388 2509098 R KEGG: sbc:SbBS512_E1394 nitrate reductase molybdenum cofactor assembly chaperone 1; TIGRFAM: nitrate reductase molybdenum cofactor assembly chaperone; PFAM: nitrate reductase subunit delta; nitrate reductase molybdenum cofactor assembly chaperone complement(2508388..2509098) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158149 YP_003036604.1 CDS ECBD_2393 NC_012947.1 2509095 2510633 R TIGRFAM: nitrate reductase subunit beta; KEGG: sbo:SBO_1841 nitrate reductase 1 subunit beta; nitrate reductase subunit beta complement(2509095..2510633) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158150 YP_003036605.1 CDS ECBD_2394 NC_012947.1 2510630 2514373 R TIGRFAM: nitrate reductase, subunit alpha; PFAM: molybdopterin oxidoreductase; molydopterin dinucleotide-binding region; KEGG: sfv:SFV_1240 nitrate reductase 1, subunit alpha; nitrate reductase, subunit alpha complement(2510630..2514373) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158151 YP_003036606.1 CDS ECBD_2395 NC_012947.1 2514889 2516280 R TIGRFAM: nitrite transporter; PFAM: major facilitator superfamily MFS_1; KEGG: sbc:SbBS512_E1391 nitrite extrusion protein 1; nitrite transporter complement(2514889..2516280) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158152 YP_003036607.1 CDS ECBD_2396 NC_012947.1 2516375 2516593 R KEGG: ssn:SSON_1954 hypothetical protein; hypothetical protein complement(2516375..2516593) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158153 YP_003036608.1 CDS ECBD_2397 NC_012947.1 2516619 2518415 D KEGG: sfv:SFV_1238 nitrate/nitrite sensor protein NarX; PFAM: ATP-binding region ATPase domain protein; histidine kinase dimerisation and phosphoacceptor region; histidine kinase HAMP region domain protein; SMART: ATP-binding region ATPase domain protein; histidine kinase HAMP region domain protein; nitrate/nitrite sensor protein NarX 2516619..2518415 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158154 YP_003036609.1 CDS ECBD_2398 NC_012947.1 2518408 2519058 D two-component response regulator NarL; phosphorylated by NarQ; activates transcription of nitrate and nitrite reductase genes and represses transcription of fumarate reductase; transcriptional regulator NarL 2518408..2519058 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160209 YP_003036610.1 CDS ECBD_2399 NC_012947.1 2519059 2520489 R KEGG: elf:LF82_2746 uncharacterized protein YchO; hypothetical protein complement(2519059..2520489) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160292 YP_003036611.1 CDS ECBD_2400 NC_012947.1 2520639 2520992 D PFAM: DsrE family protein; KEGG: sdy:SDY_1271 hypothetical protein; DsrE family protein 2520639..2520992 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160293 YP_003036612.1 CDS ECBD_2401 NC_012947.1 2521036 2521731 R PFAM: cation transport protein ChaC; KEGG: sbc:SbBS512_E1385 ChaC protein; cation transport protein ChaC complement(2521036..2521731) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160294 YP_003036613.1 CDS chaB NC_012947.1 2521889 2522119 R in Escherichia coli this protein ly regulates the sodium/proton (also pH-independent calcium/proton) antiporter chaA; has weak affinity for calcium and magnesium ions; cation transport regulator complement(2521889..2522119) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160295 YP_003036614.1 CDS ECBD_2403 NC_012947.1 2522389 2523489 D TIGRFAM: calcium/proton exchanger; PFAM: sodium/calcium exchanger membrane region; KEGG: ssn:SSON_1961 calcium/sodium:proton antiporter; calcium/sodium:proton antiporter 2522389..2523489 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160296 YP_003036615.1 CDS ECBD_2404 NC_012947.1 2524429 2524536 D KEGG: ssn:SSON_1962 hypothetical protein; hypothetical protein 2524429..2524536 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160297 YP_003036616.1 CDS ECBD_2405 NC_012947.1 2524937 2525071 D KEGG: sbo:SBO_1851 hypothetical protein; hypothetical protein 2524937..2525071 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160298 YP_003036617.1 CDS ECBD_2406 NC_012947.1 2525218 2526072 R catalyzes the formation of 2-dehydro-3-deoxy-D-octonate 8-phosphate from phosphoenolpyruvate and D-arabinose 5-phosphate in LPS biosynthesis; 2-dehydro-3-deoxyphosphooctonate aldolase complement(2525218..2526072) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160299 YP_003036618.1 CDS ECBD_2407 NC_012947.1 2526108 2526917 R KEGG: sbc:SbBS512_E1378 sirB1 protein; transcriptional regulator complement(2526108..2526917) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160300 YP_003036619.1 CDS ECBD_2408 NC_012947.1 2526921 2527313 R PFAM: invasion gene expression up-regulator SirB; KEGG: ssn:SSON_1965 transcriptional regulator; transcriptional regulator complement(2526921..2527313) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160301 YP_003036620.1 CDS ECBD_2409 NC_012947.1 2527310 2528143 R HemK; PrmC; transfers a methyl group from S-adenosylmethionine to amide nitrogen of specific glutamine residues in protein chain release factors PrfA and PrfB; N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase complement(2527310..2528143) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160302 YP_003036621.1 CDS prfA NC_012947.1 2528143 2529225 R recognizes the termination signals UAG and UAA during protein translation a specificity which is dependent on amino acid residues residing in loops of the L-shaped tRNA-like molecule of RF1; this protein is similar to release factor 2; peptide chain release factor 1 complement(2528143..2529225) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160303 YP_003036622.1 CDS hemA NC_012947.1 2529267 2530523 R catalyzes the formation of glutamate-1-semialdehyde from glutamyl-tRNA(Glu) and NADPH; the second step of the pathway is catalyzed by glutamate-1-semialdehyde aminomutase which results in the formation of 5-aminolevulinic acid; functions in porphyrin (tetrapyrroles) biosynthesis; the crystal structure showed a C-terminal dimerization domain that appears to be absent in Chlamydial proteins; glutamyl-tRNA reductase complement(2529267..2530523) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160304 YP_003036623.1 CDS lolB NC_012947.1 2530737 2531360 D Incorporates lipoproteins in the outer membrane after they are released by the LolA protein; outer membrane lipoprotein LolB 2530737..2531360 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160305 YP_003036624.1 CDS ipk NC_012947.1 2531360 2532211 D An essential enzyme in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis; 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase 2531360..2532211 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160306 YP_003036625.1 CDS ECBD_2414 NC_012947.1 2532296 2533309 D catalyzes the formation of 5-phospho-alpha-D-ribose 1-phosphate from D-ribose 5-phosphate and ATP; ribose-phosphate pyrophosphokinase 2532296..2533309 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160307 YP_003036626.1 CDS ECBD_2415 NC_012947.1 2533461 2535113 D role in sulfate transport across the inner membrane; member of the SulP family of sulfate transporters; seems to mediate transport via sulfate/proton symport; sulfate transporter YchM 2533461..2535113 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160308 YP_003036627.1 CDS ECBD_2416 NC_012947.1 2535168 2535446 R YchH; transcription activated by CRP (cyclic AMP receptor protein), a global transcription factor involved in regulation of metabolism in enteric bacteria; ychH presents a class II promoter to bind CRP; unknown function; hypothetical protein complement(2535168..2535446) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160309 YP_003036628.1 CDS ECBD_2417 NC_012947.1 2535724 2536308 D Enables the recycling of peptidyl-tRNAs produced at termination of translation; peptidyl-tRNA hydrolase 2535724..2536308 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160310 YP_003036629.1 CDS ECBD_2418 NC_012947.1 2536425 2537516 D translation-associated GTPase; the crystal structure of the Haemophilus influenzae YchF protein showed similarity to the yeast structure (PDB: 1NI3); fluorescence spectroscopy revealed nucleic acid binding; the yeast protein YBR025c interacts with the translation elongation factor eEF1; GTP-dependent nucleic acid-binding protein EngD 2536425..2537516 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160311 YP_003036630.1 CDS ECBD_2419 NC_012947.1 2537761 2537940 R KEGG: sbc:SbBS512_E1366 hypothetical protein; hypothetical protein complement(2537761..2537940) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160312 YP_003036631.1 CDS ECBD_2420 NC_012947.1 2538285 2541152 D TIGRFAM: outer membrane autotransporter barrel domain protein; PFAM: Pertactin; Autotransporter beta- domain protein; KEGG: sfx:S1289 adhesion and penetration protein; outer membrane autotransporter barrel domain protein 2538285..2541152 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160313 YP_003036632.1 CDS ECBD_2421 NC_012947.1 2541252 2543171 R Positively regulates the dhaKLM operon from a sigma-70 promoter; DNA-binding transcriptional regulator DhaR complement(2541252..2543171) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160314 YP_003036633.1 CDS ECBD_2422 NC_012947.1 2543399 2544469 D with DhaL and DhaM forms dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone; DhaK is the dihydroxyacetone binding subunit of the dihydroxyacetone kinase; dihydroxyacetone kinase subunit DhaK 2543399..2544469 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160315 YP_003036634.1 CDS ECBD_2423 NC_012947.1 2544480 2545112 D with DhaK and DhaM catalyzes the phosphorylation of dihydroxyacetone; dihydroxyacetone kinase subunit DhaL 2544480..2545112 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160316 YP_003036635.1 CDS ECBD_2424 NC_012947.1 2545123 2546541 D phosphotransferaese subunit; phosphorylates dihydroxyacetone along with DhaK/DhaL; dihydroxyacetone kinase subunit M 2545123..2546541 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160317 YP_003036636.1 CDS treA NC_012947.1 2546861 2548558 D periplasmic; catalyzes the hydrolysis of trehalose to glucose which can then be imported into the cell; trehalase 2546861..2548558 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160318 YP_003036637.1 CDS ECBD_2426 NC_012947.1 2548637 2549077 R KEGG: sbc:SbBS512_E1354 hypothetical protein; hypothetical protein complement(2548637..2549077) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160319 YP_003036638.1 CDS ECBD_2427 NC_012947.1 2549255 2549509 R PFAM: transglycosylase; KEGG: sdy:SDY_1234 hypothetical protein; transglycosylase complement(2549255..2549509) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160320 YP_003036639.1 CDS ECBD_2428 NC_012947.1 2549710 2550444 D PFAM: YcgR family protein; type IV pilus assembly PilZ; KEGG: ssn:SSON_1186 hypothetical protein; YcgR family protein 2549710..2550444 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160321 YP_003036640.1 CDS ECBD_2429 NC_012947.1 2550446 2551057 R PFAM: lytic transglycosylase catalytic; KEGG: ssn:SSON_1184 murein transglycosylase E; lytic transglycosylase catalytic complement(2550446..2551057) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160322 YP_003036641.1 CDS ldcA NC_012947.1 2551157 2552071 D catalyzes the release of D-alanine from L-alanyl-D-glutamyl-meso-diaminopimelyl-D-alanine; L,D-carboxypeptidase A 2551157..2552071 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160323 YP_003036642.1 CDS ECBD_2431 NC_012947.1 2552166 2553902 D the Vibrio parahaemolyticus gene VP2867 was found to be a potassium/proton antiporter; can rapidly extrude potassium against a potassium gradient at alkaline pH when cloned and expressed in Escherichia coli; potassium/proton antiporter 2552166..2553902 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160324 YP_003036643.1 CDS dadX NC_012947.1 2554288 2555358 R catabolic enzyme, converts L-alanine to D-alanine which is then oxidized to pyruvate by dadA; alanine racemase complement(2554288..2555358) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160325 YP_003036644.1 CDS ECBD_2433 NC_012947.1 2555368 2556666 R catalyzes the oxidative deamination of D-amino acids; D-amino acid dehydrogenase small subunit complement(2555368..2556666) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160326 YP_003036645.1 CDS ECBD_2434 NC_012947.1 2556996 2558528 D PFAM: SpoVR family protein; SpoVR family protein 2556996..2558528 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160327 YP_003036646.1 CDS ECBD_2435 NC_012947.1 2558580 2559299 R Multifunctional regulator of fatty acid metabolism; fatty acid metabolism regulator complement(2558580..2559299) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160328 YP_003036647.1 CDS nhaB NC_012947.1 2559520 2561061 D involved in regulation of intracellular pH under alkaline conditions; sodium/proton antiporter 2559520..2561061 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160329 YP_003036648.1 CDS ECBD_2437 NC_012947.1 2561207 2561737 D disulfide oxidoreductase; integral membrane protein; required for perioplasmic disulfide bond formation; oxidizes DsbA protein; disulfide bond formation protein B 2561207..2561737 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160330 YP_003036649.1 CDS umuC NC_012947.1 2561783 2563051 R binds processed UmuD protein to form functional DNA pol V (UmuD'2UmuC); involved in translesion polymerization; DNA polymerase V subunit UmuC complement(2561783..2563051) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160331 YP_003036650.1 CDS ECBD_2439 NC_012947.1 2563051 2563470 R binds with UmuC protein to form functional DNA pol V (UmuD'2UmuC); involved in translesion polymerization; DNA polymerase V subunit UmuD complement(2563051..2563470) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160332 YP_003036651.1 CDS ECBD_2441 NC_012947.1 2564950 2565396 R PFAM: protein of unknown function UPF0153; KEGG: sfv:SFV_1188 hypothetical protein; hypothetical protein complement(2564950..2565396) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160334 YP_003036652.1 CDS ECBD_2442 NC_012947.1 2565488 2566147 R PFAM: fumarylacetoacetate hydrolase; KEGG: sbc:SbBS512_E1335 fumarylacetoacetate hydrolase family protein; hypothetical protein complement(2565488..2566147) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160335 YP_003036653.1 CDS ECBD_2443 NC_012947.1 2566219 2566512 R PFAM: protein of unknown function DUF709; KEGG: ssn:SSON_1168 hypothetical protein; hypothetical protein complement(2566219..2566512) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160336 YP_003036654.1 CDS ECBD_2444 NC_012947.1 2566754 2567155 D PFAM: peptidase domain protein; KEGG: elf:LF82_2732 uncharacterized protein YcgK; peptidase domain protein 2566754..2567155 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160337 YP_003036655.1 CDS ECBD_2445 NC_012947.1 2567258 2567626 R PFAM: Fels-1 Propage domain protein; Fels-1 Propage domain protein complement(2567258..2567626) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160338 YP_003036656.1 CDS minC NC_012947.1 2568146 2568841 D blocks the formation of polar Z-ring septums; septum formation inhibitor 2568146..2568841 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160339 YP_003036657.1 CDS ECBD_2447 NC_012947.1 2568865 2569677 D ATPase; with MinC inhibits cell division by blocking formation of the polar Z ring septums; cell division inhibitor MinD 2568865..2569677 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160340 YP_003036658.1 CDS minE NC_012947.1 2569681 2569947 D works in conjunction with MinC and MinD to enable cell division at the midpoint of the long axis of the cell; cell division topological specificity factor MinE 2569681..2569947 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160341 YP_003036659.1 CDS ECBD_2450 NC_012947.1 2571298 2571582 D KEGG: sfl:SF1159 hypothetical protein; hypothetical protein 2571298..2571582 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160343 YP_003036660.1 CDS ECBD_2451 NC_012947.1 2571592 2571921 D KEGG: ssn:SSON_1161 hypothetical protein; hypothetical protein 2571592..2571921 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160344 YP_003036661.1 CDS ECBD_2453 NC_012947.1 2575025 2575243 R KEGG: elf:LF82_3545 uncharacterized protein YmgF; hypothetical protein complement(2575025..2575243) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160346 YP_003036662.1 CDS ECBD_2454 NC_012947.1 2575375 2576940 R PFAM: EAL domain protein; SMART: EAL domain protein; KEGG: sfx:S1241 hypothetical protein; diguanylate phosphodiesterase complement(2575375..2576940) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160347 YP_003036663.1 CDS ECBD_2455 NC_012947.1 2577230 2577478 R KEGG: elf:LF82_3542 uncharacterized protein YmgC; hypothetical protein complement(2577230..2577478) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160348 YP_003036664.1 CDS ECBD_2456 NC_012947.1 2577591 2577857 R hypothetical protein complement(2577591..2577857) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160349 YP_003036665.1 CDS ECBD_2457 NC_012947.1 2577886 2578158 R KEGG: elf:LF82_3540 hypothetical protein; hypothetical protein complement(2577886..2578158) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160350 YP_003036666.1 CDS ECBD_2458 NC_012947.1 2578201 2578437 R hypothetical protein complement(2578201..2578437) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160351 YP_003036667.1 CDS ECBD_2459 NC_012947.1 2578751 2579962 D PFAM: EAL domain protein; BLUF domain protein; SMART: EAL domain protein; diguanylate phosphodiesterase 2578751..2579962 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160352 YP_003036668.1 CDS ECBD_2460 NC_012947.1 2580167 2580898 D PFAM: regulatory protein MerR; SMART: regulatory protein MerR; KEGG: sbc:SbBS512_E1317 transcriptional regulator MlrA; MerR family transcriptional regulator 2580167..2580898 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160353 YP_003036669.1 CDS ECBD_2461 NC_012947.1 2581119 2581523 D PFAM: protein of unknown function DUF1398; hypothetical protein 2581119..2581523 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160354 YP_003036670.1 CDS ECBD_2462 NC_012947.1 2582219 2582542 D KEGG: sdy:SDY_2015 hypothetical protein; hypothetical protein 2582219..2582542 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160355 YP_003036671.1 CDS ECBD_2463 NC_012947.1 2582645 2583895 R Converts isocitrate to alpha ketoglutarate; isocitrate dehydrogenase complement(2582645..2583895) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160356 YP_003036672.1 CDS ECBD_2464 NC_012947.1 2584067 2584720 D catalyzes the formation of pseudouridine from uracil-2457 in 23S ribosomal RNA; 23S rRNA pseudouridine synthase E 2584067..2584720 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160357 YP_003036673.1 CDS ECBD_2465 NC_012947.1 2584730 2585191 D PFAM: NUDIX hydrolase; KEGG: sfv:SFV_1169 phosphohydrolase; NUDIX hydrolase 2584730..2585191 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160358 YP_003036674.1 CDS mnmA NC_012947.1 2585245 2586351 D catalyzes a sulfuration reaction to synthesize 2-thiouridine at the U34 position of tRNAs; tRNA-specific 2-thiouridylase MnmA 2585245..2586351 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160359 YP_003036675.1 CDS ECBD_2467 NC_012947.1 2586387 2587028 D HflD; UPF0274; in Escherichia coli this protein is peripherally associated with the membrane and appears to act with lambda CII protein; in Haemophilus influenzae a knockout of the HI0638 gene affected paracytosis; hypothetical protein 2586387..2587028 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160360 YP_003036676.1 CDS ECBD_2468 NC_012947.1 2587032 2588402 D Catalyzes two discrete reactions in the de novo synthesis of purines: the cleavage of adenylosuccinate and succinylaminoimidazole carboxamide ribotide; adenylosuccinate lyase 2587032..2588402 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160361 YP_003036677.1 CDS ECBD_2469 NC_012947.1 2588570 2589241 D response regulator in two-component regulatory system with PhoQ; involved in magnesium starvation and stress; DNA-binding transcriptional regulator PhoP 2588570..2589241 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160362 YP_003036678.1 CDS ECBD_2470 NC_012947.1 2589241 2590701 D PFAM: PhoQ Sensor domain protein; ATP-binding region ATPase domain protein; histidine kinase A domain protein; histidine kinase HAMP region domain protein; SMART: ATP-binding region ATPase domain protein; KEGG: sdy:SDY_2023 sensor protein PhoQ; sensor protein PhoQ 2589241..2590701 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160363 YP_003036679.1 CDS ECBD_2471 NC_012947.1 2590777 2591898 D PFAM: cupin; SMART: transcription factor jumonji jmjC domain protein; KEGG: sbc:SbBS512_E1306 cupin family protein; cupin 2590777..2591898 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160364 YP_003036680.1 CDS ECBD_2472 NC_012947.1 2591947 2593173 R catalyzes the release of the N-terminal amino acid from a tripeptide; peptidase T complement(2591947..2593173) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160365 YP_003036681.1 CDS potA NC_012947.1 2593423 2594559 D functions together with PotBCD (A2BCD) in ATP-dependent polyamine transport; PotA is the membrane-associated ATPase; putrescine/spermidine ABC transporter ATPase protein 2593423..2594559 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160366 YP_003036682.1 CDS potB NC_012947.1 2594543 2595400 D can transport spermidine and putrescine but affinity is higher for spermidine; forms a complex of PotA2BCD; PotB is a membrane component; spermidine/putrescine ABC transporter membrane protein 2594543..2595400 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160367 YP_003036683.1 CDS potC NC_012947.1 2595397 2596191 D can transport spermidine and putrescine but affinity is higher for spermidine; forms a complex of PotA2BCD; PotC is a membrane component; spermidine/putrescine ABC transporter membrane protein 2595397..2596191 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160368 YP_003036684.1 CDS potD NC_012947.1 2596188 2597234 D can transport spermidine and putrescine but affinity is higher for spermidine; forms a complex of PotA2BCD; PotD is a periplasmic component that binds the substrate; spermidine/putrescine ABC transporter substrate-binding protein 2596188..2597234 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160369 YP_003036685.1 CDS ECBD_2477 NC_012947.1 2597292 2597699 D KEGG: efe:EFER_1286 conserved hypothetical protein; inner membrane protein; hypothetical proten 2597292..2597699 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160370 YP_003036686.1 CDS ECBD_2478 NC_012947.1 2597749 2598537 D KEGG: elf:LF82_2726 inner membrane protein YcfZ; inner membrane protein YcfZ 2597749..2598537 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160371 YP_003036687.1 CDS ECBD_2479 NC_012947.1 2598656 2599495 R Modulates the activities of several enzymes which are inactive in their acetylated form; NAD-dependent deacetylase complement(2598656..2599495) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160372 YP_003036688.1 CDS ECBD_2481 NC_012947.1 2600020 2600523 R PFAM: IS1 transposase; KEGG: hdu:HD1970 insertion element IS1 1/5/6 protein InsB; IS1 transposase complement(2600020..2600523) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159887 YP_003036689.1 CDS ECBD_2482 NC_012947.1 2600442 2600717 R PFAM: Insertion element protein; KEGG: hdu:HD1971 insertion element IS1 1/2/3/5/6 protein InsA; Insertion element protein complement(2600442..2600717) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159888 YP_003036690.1 CDS ECBD_2483 NC_012947.1 2601228 2602466 R part of the ATP-dependent transporter LolCDE; responsible for the localization of lipoproteins to the periplasmic surface of the outer membrane; outer membrane-specific lipoprotein transporter subunit LolE complement(2601228..2602466) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159889 YP_003036691.1 CDS lolD NC_012947.1 2602472 2603173 R outer membrane specific; part of transporter complex lolCDE involved in the translocation of mature outer membrane-directed lipoproteins, from the inner membrane to the periplasmic chaperone; lipoprotein transporter ATP-binding subunit complement(2602472..2603173) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159890 YP_003036692.1 CDS ECBD_2485 NC_012947.1 2603166 2604365 R part of the ATP-dependent transporter LolCDE; responsible for the localization of lipoproteins to the periplasmic surface of the outer membrane; outer membrane-specific lipoprotein transporter subunit LolC complement(2603166..2604365) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159891 YP_003036693.1 CDS ECBD_2486 NC_012947.1 2604627 2605700 D PFAM: acyltransferase 3; KEGG: ssn:SSON_1135 hypothetical protein; acyltransferase 3 2604627..2605700 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159892 YP_003036694.1 CDS ECBD_2487 NC_012947.1 2605780 2609274 D KEGG: sfv:SFV_1134 transcription-repair coupling factor; TIGRFAM: transcription-repair coupling factor; PFAM: TRCF domain protein; transcription factor CarD; helicase; DEAD/DEAH box helicase; type III restriction protein res subunit; SMART: DEAD-like helicase ; helicase; transcription-repair coupling factor 2605780..2609274 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159893 YP_003036695.1 CDS ECBD_2488 NC_012947.1 2609421 2610380 D PFAM: ErfK/YbiS/YcfS/YnhG family protein; peptidoglycan-binding LysM; SMART: peptidoglycan-binding LysM; KEGG: sdy:SDY_2037 hypothetical protein; ErfK/YbiS/YcfS/YnhG family protein 2609421..2610380 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159894 YP_003036696.1 CDS ECBD_2489 NC_012947.1 2610463 2610720 R PFAM: protein of unknown function DUF1471; KEGG: elf:LF82_0232 UPF0379 protein YcfR; hypothetical protein complement(2610463..2610720) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159895 YP_003036697.1 CDS ECBD_2490 NC_012947.1 2610961 2611593 D PFAM: regulatory protein TetR; KEGG: ssn:SSON_1131 hypothetical protein; TetR family transcriptional regulator 2610961..2611593 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159896 YP_003036698.1 CDS ECBD_2491 NC_012947.1 2611655 2612194 R PFAM: 17 kDa surface antigen; KEGG: ssn:SSON_1130 hypothetical protein; hypothetical protein complement(2611655..2612194) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159897 YP_003036699.1 CDS ECBD_2492 NC_012947.1 2612421 2613725 R PFAM: FAD-dependent pyridine nucleotide-disulphide oxidoreductase; FAD dependent oxidoreductase; KEGG: sfx:S1193 respiratory NADH dehydrogenase; FAD-dependent pyridine nucleotide-disulfide oxidoreductase complement(2612421..2613725) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159898 YP_003036700.1 CDS ECBD_2493 NC_012947.1 2614133 2614675 R PFAM: protein of unknown function UPF0227; KEGG: sfx:S1192 hypothetical protein; hypothetical protein complement(2614133..2614675) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159899 YP_003036701.1 CDS ECBD_2494 NC_012947.1 2614698 2615723 R hydrolyzes the terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides; beta-hexosaminidase complement(2614698..2615723) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159900 YP_003036702.1 CDS thiK NC_012947.1 2615734 2616558 R catalyzes the phosphorylation of thiamine to thiamine phosphate; thiamine kinase complement(2615734..2616558) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159901 YP_003036703.1 CDS ECBD_2496 NC_012947.1 2616539 2617180 R TIGRFAM: membrane lipoprotein lipid attachment site; KEGG: sdy:SDY_2045 hypothetical protein; membrane lipoprotein lipid attachment site complement(2616539..2617180) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159902 YP_003036704.1 CDS ECBD_2497 NC_012947.1 2617194 2617571 R PFAM: protein of unknown function DUF1425; KEGG: ssn:SSON_1124 hypothetical protein; hypothetical protein complement(2617194..2617571) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159903 YP_003036705.1 CDS ECBD_2498 NC_012947.1 2617574 2617933 R PFAM: histidine triad (HIT) protein; KEGG: ssn:SSON_1123 purine nucleoside phosphoramidase; purine nucleoside phosphoramidase complement(2617574..2617933) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159904 YP_003036706.1 CDS ECBD_2499 NC_012947.1 2618267 2620456 D TIGRFAM: TonB-dependent siderophore receptor; PFAM: TonB-dependent receptor; TonB-dependent receptor plug; KEGG: sdy:SDY_2048 ferric-rhodotorulic acid outer membrane transporter; ferric-rhodotorulic acid outer membrane transporter 2618267..2620456 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159905 YP_003036707.1 CDS ECBD_2500 NC_012947.1 2620516 2621949 R phosphoenolpyruvate-dependent sugar phosphotransferase system; catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane; IIB is phosphorylated by IIA and then transfers the phosphoryl group to the sugar; IIC forms the translocation channel; PTS system glucose-specific transporter subunits IIBC complement(2620516..2621949) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159906 YP_003036708.1 CDS ECBD_2501 NC_012947.1 2622244 2623041 R TIGRFAM: hydrolase, TatD family; PFAM: TatD-related deoxyribonuclease; KEGG: sdy:SDY_2050 predicted metallodependent hydrolase; metallodependent hydrolase complement(2622244..2623041) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159907 YP_003036709.1 CDS ECBD_2502 NC_012947.1 2623052 2624056 R catalyzes the DNA-template-directed extension of the 3'-end of a DNA strand; the delta' subunit seems to interact with the gamma subunit to transfer the subunit beta on the DNA; DNA polymerase III subunit delta' complement(2623052..2624056) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159908 YP_003036710.1 CDS tmk NC_012947.1 2624053 2624694 R catalyzes the reversible phosphoryl transfer from adenosine triphosphate (ATP) to thymidine monophosphate (dTMP) to form thymidine diphosphate (dTDP); thymidylate kinase complement(2624053..2624694) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159909 YP_003036711.1 CDS ECBD_2504 NC_012947.1 2624684 2625706 R PFAM: aminodeoxychorismate lyase; KEGG: sbc:SbBS512_E2227 hypothetical protein; hypothetical protein complement(2624684..2625706) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159910 YP_003036712.1 CDS ECBD_2505 NC_012947.1 2625709 2626518 R catalyzes the formation of 4-aminobenzoate and pyruvate from 4-amino-4-deoxychorismate; 4-amino-4-deoxychorismate lyase complement(2625709..2626518) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159911 YP_003036713.1 CDS ECBD_2506 NC_012947.1 2626638 2627879 R FabF; beta-ketoacyl-ACP synthase II, KASII; catalyzes a condensation reaction in fatty acid biosynthesis: addition of an acyl acceptor of two carbons from malonyl-ACP; required for the elongation of short-chain unsaturated acyl-ACP; 3-oxoacyl-(acyl carrier protein) synthase II complement(2626638..2627879) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159912 YP_003036714.1 CDS acpP NC_012947.1 2627967 2628203 R carries the fatty acid chain in fatty acid biosynthesis; acyl carrier protein complement(2627967..2628203) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159913 YP_003036715.1 CDS fabG NC_012947.1 2628414 2629148 R catalyzes the first of the two reduction steps in the elongation cycle of fatty acid synthesis; 3-ketoacyl-ACP reductase complement(2628414..2629148) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159914 YP_003036716.1 CDS ECBD_2509 NC_012947.1 2629161 2630090 R TIGRFAM: malonyl CoA-acyl carrier protein transacylase; PFAM: acyl transferase; KEGG: sbc:SbBS512_E2232 malonyl CoA-acyl carrier protein transacylase; malonyl CoA-acyl carrier protein transacylase complement(2629161..2630090) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159915 YP_003036717.1 CDS ECBD_2510 NC_012947.1 2630106 2631059 R FabH; beta-ketoacyl-acyl carrier protein synthase III; catalyzes the condensation of acetyl-CoA with malonyl-ACP to initiate cycles of fatty acid elongation; differs from 3-oxoacyl-(acyl carrier protein) synthase I and II in that it utilizes CoA thioesters as primers rather than acyl-ACPs; 3-oxoacyl-(acyl carrier protein) synthase III complement(2630106..2631059) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159916 YP_003036718.1 CDS ECBD_2511 NC_012947.1 2631127 2632167 R involved in acylation of glycerol-3-phosphate to form 1-acyl-glycerol-3 phosphate for use in phospholipid biosynthesis; functions with PlsY; glycerol-3-phosphate acyltransferase PlsX complement(2631127..2632167) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159917 YP_003036719.1 CDS rpmF NC_012947.1 2632278 2632451 R some L32 proteins have zinc finger motifs consisting of CXXC while others do not; 50S ribosomal protein L32 complement(2632278..2632451) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159918 YP_003036720.1 CDS ECBD_2513 NC_012947.1 2632503 2633024 R PFAM: protein of unknown function DUF177; KEGG: sdy:SDY_2062 hypothetical protein; hypothetical protein complement(2632503..2633024) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159919 YP_003036721.1 CDS ECBD_2514 NC_012947.1 2633184 2633807 D Maf; overexpression in Bacillus subtilis inhibits septation in the dividing cell; Maf-like protein 2633184..2633807 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159920 YP_003036722.1 CDS ECBD_2515 NC_012947.1 2633919 2634878 R catalyzes the transformation of uracil to pseudouracil at nucleotides U955, U2504, and U2580 in 23S rRNA; 23S rRNA pseudouridylate synthase C complement(2633919..2634878) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159921 YP_003036723.1 CDS rne NC_012947.1 2635451 2638636 D bifunctional ribonuclease E/endoribonuclease/RNA-binding protein/RNA degradosome binding protein; forms part of the membrane-associated degradosome complex along with PNPase, RhlB, and enolase; ribonuclease E 2635451..2638636 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159922 YP_003036724.1 CDS flgL NC_012947.1 2638832 2639785 R with FlgK acts as a hook filament junction protein to join the flagellar filament to the hook; Yersinia, Vibrio parahaemolyticus, Bradyrhizobium and other organisms have 2 copies of this and other flagellar genes; flagellar hook-associated protein FlgL complement(2638832..2639785) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159923 YP_003036725.1 CDS flgK NC_012947.1 2639797 2641440 R with FlgL acts as a hook filament junction protein to join the flagellar filament to the hook; flagellar hook-associated protein FlgK complement(2639797..2641440) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159924 YP_003036726.1 CDS flgJ NC_012947.1 2641506 2642447 R Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space; flagellar rod assembly protein/muramidase FlgJ complement(2641506..2642447) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159925 YP_003036727.1 CDS flgI NC_012947.1 2642447 2643544 R part of the basal body which consists of four rings L, P, S, and M mounted on a central rod; Vibrio parahaemolyticus, Yersinia, Bradyrhizobium and other bacteria have two copies of this and other flagellar genes; the V. parahaemolyticus protein is associated with the polar flagella and the Bradyrhizobium protein is associated with the thick flagellum; flagellar basal body P-ring protein complement(2642447..2643544) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159926 YP_003036728.1 CDS flgH NC_012947.1 2643556 2644263 R part of the flagellar basal body which consists of four rings L,P, S and M mounted on a central rod; flagellar basal body L-ring protein complement(2643556..2644263) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159927 YP_003036729.1 CDS flgG NC_012947.1 2644307 2645089 R makes up the distal portion of the flagellar basal body rod; flagellar basal body rod protein FlgG complement(2644307..2645089) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159928 YP_003036730.1 CDS flgF NC_012947.1 2645261 2646016 R FlgF, with FlgB and C, makes up the proximal portion of the flagellar basal body rod; flagellar basal body rod protein FlgF complement(2645261..2646016) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159929 YP_003036731.1 CDS flgE NC_012947.1 2646036 2647241 R the hook connects flagellar basal body to the flagellar filament; flagellar hook protein FlgE complement(2646036..2647241) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159930 YP_003036732.1 CDS flgD NC_012947.1 2647266 2647961 R acts as a scaffold for the assembly of hook proteins onto the flagellar basal body rod; Yersinia, Vibrio parahaemolyticus, Bradyrhizobium and other organisms have 2 copies of some flagellar genes; in V. parahaemolyticus one set used for lateral flagella production and the other is used for the polar flagella production; flagellar basal body rod modification protein complement(2647266..2647961) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159931 YP_003036733.1 CDS flgC NC_012947.1 2647973 2648377 R with FlgF and B makes up the proximal portion of the flagellar basal body rod; flagellar basal body rod protein FlgC complement(2647973..2648377) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159932 YP_003036734.1 CDS flgB NC_012947.1 2648381 2648797 R with FlgF and C makes up the proximal portion of the flagellar basal body rod; Vibrio parahaemolyticus protein is associated with the polar flagella; flagellar basal body rod protein FlgB complement(2648381..2648797) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159933 YP_003036735.1 CDS flgA NC_012947.1 2648952 2649611 D required for the assembly of the flagellar basal body P-ring; flagellar basal body P-ring biosynthesis protein FlgA 2648952..2649611 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159934 YP_003036736.1 CDS ECBD_2529 NC_012947.1 2649687 2649980 D regulates the flagellar specific sigma28 transcription factor; anti-sigma28 factor FlgM 2649687..2649980 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159935 YP_003036737.1 CDS ECBD_2530 NC_012947.1 2649985 2650401 D KEGG: ssn:SSON_1090 protein of flagellar biosynthesis; protein of flagellar biosynthesis 2649985..2650401 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159936 YP_003036738.1 CDS ECBD_2531 NC_012947.1 2650441 2651976 R TIGRFAM: integral membrane protein MviN; KEGG: sbc:SbBS512_E2257 integral membrane protein MviN; integral membrane protein MviN complement(2650441..2651976) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159937 YP_003036739.1 CDS ECBD_2532 NC_012947.1 2652086 2653009 R KEGG: elf:LF82_2713 virulence factor MviM homolog; virulence factor MviM-like protein complement(2652086..2653009) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159938 YP_003036740.1 CDS ECBD_2533 NC_012947.1 2653011 2653658 R KEGG: ssn:SSON_1087 hypothetical protein; hypothetical protein complement(2653011..2653658) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159939 YP_003036741.1 CDS ECBD_2534 NC_012947.1 2653669 2654253 R KEGG: ssn:SSON_1086 ribosomal-protein-S5-alanine N-acetyltransferase; ribosomal-protein-S5-alanine N-acetyltransferase complement(2653669..2654253) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159940 YP_003036742.1 CDS ECBD_2535 NC_012947.1 2654489 2655697 D Confers resistance to norfloxacin and enoxacin; multidrug resistance protein MdtH 2654489..2655697 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159941 YP_003036743.1 CDS ECBD_2536 NC_012947.1 2655761 2656408 D cofactor involved in the reduction of disulfides; glutaredoxin 2655761..2656408 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159942 YP_003036744.1 CDS ECBD_2537 NC_012947.1 2656542 2657102 D KEGG: sfv:SFV_1085 hypothetical protein; hypothetical protein 2656542..2657102 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159943 YP_003036745.1 CDS ECBD_2538 NC_012947.1 2657208 2658254 D catalyzes the formation of N-carbamoyl-L-aspartate from (S)-dihydroorotate in pyrimidine biosynthesis; dihydroorotase 2657208..2658254 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159944 YP_003036746.1 CDS ECBD_2539 NC_012947.1 2658328 2658573 D PFAM: DinI family protein; KEGG: ssn:SSON_1081 DNA damage-inducible protein I; DNA damage-inducible protein I 2658328..2658573 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159945 YP_003036747.1 CDS bssS NC_012947.1 2658863 2659117 D BssS; regulator of biofilm through signal secretion; disruption of this gene in Escherichia coli led to effects on biofilm formation, alteration in expression of a number of genes and mutations in bssS led to defects in indole transport and autoinducer-2 uptake and processing; biofilm formation regulatory protein BssS 2658863..2659117 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159946 YP_003036748.1 CDS solA NC_012947.1 2659232 2660350 D catalyzes the demethylation of N-methyl-L-tryptophan forming L-tryptophan and formaldehyde; FAD-binding; can also catalyze the demethylation of other N-methyl amino acids; N-methyltryptophan oxidase 2659232..2660350 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159947 YP_003036749.1 CDS ECBD_2542 NC_012947.1 2660398 2660511 D KEGG: sfv:SFV_1081 hypothetical protein; hypothetical protein 2660398..2660511 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159948 YP_003036750.1 CDS ECBD_2543 NC_012947.1 2660772 2661338 D PFAM: cytochrome B561; KEGG: sbc:SbBS512_E2271 nickel-dependent hydrogenase b-type cytochrome subunit; cytochrome B561 2660772..2661338 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159949 YP_003036751.1 CDS ECBD_2544 NC_012947.1 2661342 2661917 D PFAM: YceI family protein; KEGG: ssn:SSON_1076 hypothetical protein; hypothetical protein 2661342..2661917 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159950 YP_003036752.1 CDS ECBD_2545 NC_012947.1 2661959 2663011 R PFAM: rhodanese; SMART: Rhodanese domain protein; KEGG: sfv:SFV_1078 hypothetical protein; hypothetical protein complement(2661959..2663011) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159951 YP_003036753.1 CDS ECBD_2546 NC_012947.1 2663236 2664156 D acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA; lipid A biosynthesis lauroyl acyltransferase 2663236..2664156 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159952 YP_003036754.1 CDS ECBD_2547 NC_012947.1 2664328 2665554 D Confers resistance to fosfomycin and deoxycholate; drug efflux system protein MdtG 2664328..2665554 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159953 YP_003036755.1 CDS ECBD_2548 NC_012947.1 2665637 2666011 D KEGG: sdy:SDY_2299 hypothetical protein; hypothetical protein 2665637..2666011 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159954 YP_003036756.1 CDS ECBD_2549 NC_012947.1 2666012 2666239 R PFAM: protein of unknown function DUF1375; KEGG: sdy:SDY_2300 predicted lipoprotein; hypothetical protein complement(2666012..2666239) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159955 YP_003036757.1 CDS ECBD_2550 NC_012947.1 2666412 2668925 R necessary for biosynthesis of osmoregulated periplasmic glucans possibly involved in the transfer to the periplasmic space; glucosyltransferase MdoH complement(2666412..2668925) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159956 YP_003036758.1 CDS mdoG NC_012947.1 2668948 2670483 R involved in the biosynthesis of osmoregulated periplasmic glucans; required for the assembly of the polyglucose structure of glucan; glucan biosynthesis protein G complement(2668948..2670483) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159957 YP_003036759.1 CDS mdoC NC_012947.1 2670877 2672034 D required for the transfer of succinyl residues to the glucan backbone; glucans biosynthesis protein 2670877..2672034 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159958 YP_003036760.1 CDS ECBD_2553 NC_012947.1 2672042 2673523 R PFAM: phospholipase D/transphosphatidylase; SMART: phospholipase D/transphosphatidylase; KEGG: sfx:S1116 synthase; phospholipase D/transphosphatidylase complement(2672042..2673523) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159959 YP_003036761.1 CDS ECBD_2554 NC_012947.1 2673465 2673998 R PFAM: Appr-1-p processing domain protein; SMART: Appr-1-p processing domain protein; KEGG: ssn:SSON_1058 hypothetical protein; hypothetical protein complement(2673465..2673998) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159960 YP_003036762.1 CDS ECBD_2555 NC_012947.1 2674093 2674404 R KEGG: ssn:SSON_1057 hypothetical protein; hypothetical protein complement(2674093..2674404) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159961 YP_003036763.1 CDS csgC NC_012947.1 2674525 2674857 R involved in autoagglutination of curliated cells; not involved in production of curli fibers; autoagglutination protein complement(2674525..2674857) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159962 YP_003036764.1 CDS ECBD_2557 NC_012947.1 2674916 2675371 R PFAM: Curlin associated repeat protein; KEGG: sbo:SBO_2026 cryptic curlin major subunit; cryptic curlin major subunit complement(2674916..2675371) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159963 YP_003036765.1 CDS ECBD_2558 NC_012947.1 2675412 2675867 R CsgB; functions as a nucleator in the assembly of curli (coiled surface structures) on the cell surface; curlin minor subunit complement(2675412..2675867) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159964 YP_003036766.1 CDS ECBD_2559 NC_012947.1 2676622 2677272 D activates the csgBA and csgDEFG operons involved in biofilm formation; DNA-binding transcriptional regulator CsgD 2676622..2677272 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159965 YP_003036767.1 CDS ECBD_2560 NC_012947.1 2677277 2677666 D chaperone-like protein that participates in the polymerization of curlin (CsgA) subunits into curli (extracellular fibers from Escherichia and Salmonella spp. that are involved in the colonization of inert surfaces and biofilm formation); part of the curli secretion and assembly protein complex; curli assembly protein CsgE 2677277..2677666 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159966 YP_003036768.1 CDS ECBD_2561 NC_012947.1 2677691 2678107 D nucleator protein that participates in the polymerization of curlin (CsgA) subunits into curli (extracellular fibers from Escherichia and Salmonella spp. that are involved in the colonization of inert surfaces and biofilm formation); part of the curli secretion and assembly protein complex; curli assembly protein CsgF 2677691..2678107 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159967 YP_003036769.1 CDS ECBD_2562 NC_012947.1 2678134 2678967 D PFAM: Curli production assembly/transport component CsgG; Curli production assembly/transport component CsgG 2678134..2678967 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160291 YP_003036770.1 CDS ECBD_2563 NC_012947.1 2679031 2679552 R PFAM: protein of unknown function DUF1097; KEGG: sbo:SBO_2032 hypothetical protein; hypothetical protein complement(2679031..2679552) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159805 YP_003036771.1 CDS ECBD_2564 NC_012947.1 2679624 2680178 R PFAM: cytoplasmic chaperone TorD family protein; KEGG: sbc:SbBS512_E2294 chaperone, TorD family; cytoplasmic chaperone TorD family protein complement(2679624..2680178) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159806 YP_003036772.1 CDS ECBD_2565 NC_012947.1 2680202 2680939 R PFAM: PHP domain protein; SMART: phosphoesterase PHP domain protein; KEGG: sbc:SbBS512_E2295 PHP domain protein; hydrolase complement(2680202..2680939) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159807 YP_003036773.1 CDS ECBD_2566 NC_012947.1 2680994 2681932 R PFAM: D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding; KEGG: ssn:SSON_1042 dehydrogenase; D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding complement(2680994..2681932) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159808 YP_003036774.1 CDS ECBD_2567 NC_012947.1 2682665 2682964 D PFAM: transposase IS3/IS911 family protein; transposase IS3/IS911 family protein 2682665..2682964 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159810 YP_003036775.1 CDS ECBD_2568 NC_012947.1 2682961 2683827 D PFAM: Integrase catalytic region; KEGG: seh:SeHA_C4704 ISEc16, orfB; Integrase catalytic region 2682961..2683827 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159811 YP_003036776.1 CDS ECBD_2569 NC_012947.1 2683868 2685226 R TIGRFAM: diguanylate cyclase; SMART: GGDEF domain containing protein; KEGG: elf:LF82_2700 inner membrane protein YcdT; diguanylate cyclase complement(2683868..2685226) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159812 YP_003036777.1 CDS pgaA NC_012947.1 2685813 2688236 D outer membrane protein essential for the synthesis of poly-beta-1,6-N-acetyl-D-glucosamine (PGA); adhesin required for biofilm formation; PgaA (HmsH) seems to translocate and/or dock PGA to the cell surface; member of the hmsHFRS (ycdSRQP or pgaABCD in Escherichia coli) operon in Yersinia; outer membrane protein PgaA 2685813..2688236 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159813 YP_003036778.1 CDS pgaB NC_012947.1 2688245 2690263 D in Yersinia this protein is involved in biofilm formation and hemin adsorption; related protein PgaB in Escherichia coli is an outer membrane N-deacetylase involved in poly-beta-1,6-N-acetyl-D-glucosamine (PGA) export; outer membrane N-deacetylase 2688245..2690263 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159814 YP_003036779.1 CDS ECBD_2572 NC_012947.1 2690256 2691581 D predicted polysaccharide polymerase involved in biofilm formation; required for the synthesis of the beta-1,6-N-acetylglucosamine polysaccharide; PgaC; in Yersinia the HmsR protein is an inner membrane protein; N-glycosyltransferase 2690256..2691581 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159815 YP_003036780.1 CDS pgaD NC_012947.1 2691583 2691996 D in Yersinia this gene is involved in biofilm formation and hemin adsorption; in Escherichia coli it is involved in PGA biosynthesis and export; PGA biosynthesis protein 2691583..2691996 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159816 YP_003036781.1 CDS ECBD_2574 NC_012947.1 2692046 2693110 R KEGG: sbc:SbBS512_E2301 protein PhoH; hypothetical protein complement(2692046..2693110) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159817 YP_003036782.1 CDS ECBD_2575 NC_012947.1 2693455 2694726 R TIGRFAM: Dyp-type peroxidase family; KEGG: sbo:SBO_2039 hypothetical protein; Dyp-type peroxidase family complement(2693455..2694726) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159818 YP_003036783.1 CDS ECBD_2576 NC_012947.1 2694732 2695859 R KEGG: ssn:SSON_1037 hypothetical protein; hypothetical protein complement(2694732..2695859) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159819 YP_003036784.1 CDS ECBD_2577 NC_012947.1 2695917 2696747 R TIGRFAM: FTR1 family protein; KEGG: ssn:SSON_1036 high-affinity iron permease; FTR1 family protein complement(2695917..2696747) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159820 YP_003036785.1 CDS ECBD_2578 NC_012947.1 2697290 2698798 R TIGRFAM: sodium/proline symporter; SSS sodium solute transporter superfamily; KEGG: sbo:SBO_2042 major sodium/proline symporter; sodium/proline symporter complement(2697290..2698798) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159821 YP_003036786.1 CDS ECBD_2579 NC_012947.1 2698957 2699166 R KEGG: sbc:SbBS512_E2303 hypothetical protein; hypothetical protein complement(2698957..2699166) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159822 YP_003036787.1 CDS putA NC_012947.1 2699221 2703183 D proline utilization protein A; multifunctional protein that functions in proline catabolism in the first two enzymatic steps resulting in the conversion of proline to glutamate; in Escherichai coli this protein also self-regulates transcription via a DNA-binding domain at the N-terminus; forms dimers and is a peripherally membrane-associated protein; trifunctional transcriptional regulator/proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase 2699221..2703183 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159823 YP_003036788.1 CDS ECBD_2581 NC_012947.1 2703223 2703861 R PFAM: Tetracycline transcriptional regulator YcdC domain protein; regulatory protein TetR; KEGG: sbc:SbBS512_E2305 transcriptional regulator RutR; TetR family transcriptional regulator complement(2703223..2703861) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159824 YP_003036789.1 CDS ECBD_2582 NC_012947.1 2704149 2705240 D PFAM: Luciferase-like monooxygenase; Luciferase-like monooxygenase 2704149..2705240 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159825 YP_003036790.1 CDS ECBD_2583 NC_012947.1 2705237 2705932 D PFAM: isochorismatase hydrolase; KEGG: ssn:SSON_1030 synthetase; isochorismatase hydrolase 2705237..2705932 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159826 YP_003036791.1 CDS ECBD_2584 NC_012947.1 2705944 2706330 D PFAM: endoribonuclease L-PSP; KEGG: sdy:SDY_0985 hypothetical protein; endoribonuclease L-PSP 2705944..2706330 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159827 YP_003036792.1 CDS ECBD_2585 NC_012947.1 2706338 2707138 D PFAM: alpha/beta hydrolase fold; KEGG: ssn:SSON_1027 acetyltransferase; alpha/beta hydrolase fold protein 2706338..2707138 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159828 YP_003036793.1 CDS ECBD_2586 NC_012947.1 2707148 2707738 D PFAM: nitroreductase; KEGG: elf:LF82_2053 NadH dehydrogenase/NAD(P)H nitroreductase RutE; hypothetical protein 2707148..2707738 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159829 YP_003036794.1 CDS ECBD_2587 NC_012947.1 2707749 2708243 D PFAM: flavin reductase domain protein FMN-binding; KEGG: elf:LF82_2054 flavin reductase RutF; flavin reductase domain protein FMN-binding 2707749..2708243 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159830 YP_003036795.1 CDS ECBD_2588 NC_012947.1 2708264 2709592 D TIGRFAM: uracil-xanthine permease; PFAM: xanthine/uracil/vitamin C permease; KEGG: ssn:SSON_1024 transport protein; uracil-xanthine permease 2708264..2709592 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159831 YP_003036796.1 CDS ECBD_2589 NC_012947.1 2709850 2710023 R KEGG: elf:LF82_3538 uncharacterized protein YmdF; hypothetical protein complement(2709850..2710023) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159832 YP_003036797.1 CDS ECBD_2590 NC_012947.1 2710396 2710992 D stationary phase protein that binds TrpR repressor; TrpR binding protein WrbA 2710396..2710992 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159833 YP_003036798.1 CDS ECBD_2591 NC_012947.1 2711013 2711240 D KEGG: sfv:SFV_1015 hypothetical protein; hypothetical protein 2711013..2711240 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159834 YP_003036799.1 CDS ECBD_2592 NC_012947.1 2711278 2712519 R PFAM: histidine acid phosphatase; KEGG: ssn:SSON_1012 glucose-1-phosphatase/inositol phosphatase; glucose-1-phosphatase/inositol phosphatase complement(2711278..2712519) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159835 YP_003036800.1 CDS ECBD_2593 NC_012947.1 2712802 2714061 R KEGG: sfx:S1074 hypothetical protein; hypothetical protein complement(2712802..2714061) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159836 YP_003036801.1 CDS ECBD_2594 NC_012947.1 2714322 2715242 D functional analog of DnaJ; co-chaperone with DnaK, molecular chaperone in an adaptive response to environmental stresses other than heat shock; curved DNA-binding protein CbpA 2714322..2715242 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159837 YP_003036802.1 CDS ECBD_2595 NC_012947.1 2715242 2715547 D with CpbA modulates the activity of the dnaK chaperone system; interacts with CbpA and inhibits both the DnaJ-like co-chaperone activity and the DNA binding activity of CbpA; chaperone-modulator protein CbpM 2715242..2715547 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159838 YP_003036803.1 CDS torD NC_012947.1 2715699 2716298 R TorD; involved in the biogenesis of torA; acts on torA before the insertion of the molybdenum cofactor and, as a result, probably favors a conformation of the apoenzyme that is competent for acquiring the cofactor; chaperone protein TorD complement(2715699..2716298) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159839 YP_003036804.1 CDS ECBD_2597 NC_012947.1 2716295 2718841 R KEGG: sfv:SFV_1007 trimethylamine N-oxide reductase subunit; TIGRFAM: molybdopterin guanine dinucleotide-containing S/N-oxide reductase; trimethylamine-N-oxide reductase TorA; PFAM: molybdopterin oxidoreductase; molydopterin dinucleotide-binding region; molybdopterin guanine dinucleotide-containing S/N-oxide reductase complement(2716295..2718841) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159840 YP_003036805.1 CDS ECBD_2598 NC_012947.1 2718841 2720013 R TIGRFAM: trimethylamine-N-oxide reductase c-type cytochrome TorC; PFAM: NapC/NirT cytochrome C domain protein; KEGG: efe:EFER_1191 trimethylamine N-oxide (TMAO) reductase I, cytochrome C subunit; trimethylamine-N-oxide reductase c-type cytochrome TorC complement(2718841..2720013) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159841 YP_003036806.1 CDS ECBD_2599 NC_012947.1 2720143 2720835 D response regulator in two-component regulatory system with TorS; involved in regulation of trimethylamine N-oxide reductase genes; DNA-binding transcriptional regulator TorR 2720143..2720835 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159842 YP_003036807.1 CDS ECBD_2600 NC_012947.1 2720808 2721836 R periplasmic sensory protein associated with the TorRS two-component regulatory system; TMAO reductase system periplasmic protein TorT complement(2720808..2721836) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159843 YP_003036808.1 CDS ECBD_2601 NC_012947.1 2721964 2724663 D Member of the two-component regulatory system torS/torR involved in the anaerobic utilization of trimethylamine-N-oxide; hybrid sensory histidine kinase TorS 2721964..2724663 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159844 YP_003036809.1 CDS ECBD_2602 NC_012947.1 2724735 2725808 D PFAM: 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: sbc:SbBS512_E2322 4Fe-4S binding domain protein; 4Fe-4S ferredoxin iron-sulfur binding domain protein 2724735..2725808 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159845 YP_003036810.1 CDS ECBD_2603 NC_012947.1 2725856 2726029 R PFAM: GnsAGnsB family protein; KEGG: ssn:SSON_0999 GnsA; GnsAGnsB family protein complement(2725856..2726029) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159846 YP_003036811.1 CDS ECBD_2604 NC_012947.1 2726019 2726249 R KEGG: ssn:SSON_0998 cold shock gene; cold shock gene complement(2726019..2726249) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159847 YP_003036812.1 CDS ECBD_2605 NC_012947.1 2726423 2726635 R PFAM: Cold-shock protein DNA-binding; SMART: Cold shock protein; KEGG: ssn:SSON_0997 DNA-binding transcriptional regulator; cold shock protein CspG complement(2726423..2726635) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159848 YP_003036813.1 CDS ECBD_2606 NC_012947.1 2726921 2727133 D PFAM: Cold-shock protein DNA-binding; SMART: Cold shock protein; KEGG: sdy:SDY_0964 cold shock-like protein; cold-shock DNA-binding domain protein 2726921..2727133 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159849 YP_003036814.1 CDS ECBD_2607 NC_012947.1 2727552 2727857 D KEGG: sbc:SbBS512_E2328 inner membrane protein; inner membrane protein 2727552..2727857 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159850 YP_003036815.1 CDS ECBD_2608 NC_012947.1 2727964 2728608 D KEGG: ssn:SSON_0993 regulator; regulator 2727964..2728608 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159851 YP_003036816.1 CDS ECBD_2609 NC_012947.1 2728605 2729351 D PFAM: protein of unknown function DUF1017; KEGG: ssn:SSON_0992 hypothetical protein; hypothetical protein 2728605..2729351 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159852 YP_003036817.1 CDS ECBD_2610 NC_012947.1 2729351 2731447 D PFAM: protein of unknown function DUF940 membrane lipoprotein KEGG: sbo:SBO_2246 hypothetical protein; hypothetical protein 2729351..2731447 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159853 YP_003036818.1 CDS ECBD_2611 NC_012947.1 2731472 2732632 D PFAM: polysaccharide export protein; KEGG: sfv:SFV_0992 function in exopolysaccharide production; polysaccharide export protein 2731472..2732632 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159854 YP_003036819.1 CDS etp NC_012947.1 2732620 2733066 D Etk and Etp are a tyrosine kinase-tyrosine phosphatase pair, etp dephosphorylates etk; phosphotyrosine-protein phosphatase 2732620..2733066 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159855 YP_003036820.1 CDS ECBD_2613 NC_012947.1 2733086 2735266 D KEGG: sbo:SBO_2249 cryptic autophosphorylating protein tyrosine kinase Etk; TIGRFAM: capsular exopolysaccharide family; PFAM: lipopolysaccharide biosynthesis protein; cryptic autophosphorylating protein tyrosine kinase Etk 2733086..2735266 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159856 YP_003036821.1 CDS ECBD_2614 NC_012947.1 2735381 2736679 R PFAM: histidine acid phosphatase; KEGG: ssn:SSON_0987 phosphoanhydride phosphorylase; phosphoanhydride phosphorylase complement(2735381..2736679) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159857 YP_003036822.1 CDS ECBD_2615 NC_012947.1 2736759 2736851 R expressed during stationary phase; membrane protein complement(2736759..2736851) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159858 YP_003036823.1 CDS ECBD_2616 NC_012947.1 2736864 2738000 R TIGRFAM: cytochrome d ubiquinol oxidase subunit II; PFAM: cytochrome bd ubiquinol oxidase subunit II; KEGG: ssn:SSON_0985 probable third cytochrome oxidase subunit II; cytochrome d ubiquinol oxidase subunit II complement(2736864..2738000) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159859 YP_003036824.1 CDS ECBD_2617 NC_012947.1 2738012 2739556 R PFAM: cytochrome bd ubiquinol oxidase subunit I; KEGG: sfv:SFV_0987 third cytochrome oxidase subunit I; cytochrome bd ubiquinol oxidase subunit I complement(2738012..2739556) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159860 YP_003036825.1 CDS ECBD_2618 NC_012947.1 2739690 2740547 R PFAM: HupH hydrogenase expression protein; KEGG: sbc:SbBS512_E2338 hydrogenase-1 operon protein HyaF; HupH hydrogenase expression protein complement(2739690..2740547) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159861 YP_003036826.1 CDS ECBD_2619 NC_012947.1 2740544 2740942 R involved in processing hydrogenase proteins HyaA and HyaB; hydrogenase-1 operon protein HyaE complement(2740544..2740942) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159862 YP_003036827.1 CDS ECBD_2620 NC_012947.1 2740939 2741526 R HyaD; endopeptidase involved in the cleavage of the C-terminus end of HyaB (the large subunit of hydrogenase 1); hydrogenase 1 maturation protease complement(2740939..2741526) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159863 YP_003036828.1 CDS ECBD_2621 NC_012947.1 2741523 2742230 R TIGRFAM: Ni/Fe-hydrogenase, b-type cytochrome subunit; PFAM: cytochrome B561; KEGG: sdy:SDY_0949 hydrogenase 1 b-type cytochrome subunit; hydrogenase 1 b-type cytochrome subunit complement(2741523..2742230) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159864 YP_003036829.1 CDS ECBD_2622 NC_012947.1 2742249 2744042 R involved in hydrogen cycling during fermentative growth; hydrogenase 1 large subunit complement(2742249..2744042) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159865 YP_003036830.1 CDS ECBD_2623 NC_012947.1 2744039 2745157 R KEGG: sdy:SDY_0947 hydrogenase-1 small subunit; TIGRFAM: hydrogenase (NiFe) small subunit HydA; PFAM: NADH ubiquinone oxidoreductase 20 kDa subunit; hydrogenase (NiFe) small subunit HydA complement(2744039..2745157) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159866 YP_003036831.1 CDS ECBD_2624 NC_012947.1 2745878 2746537 D PFAM: protein of unknown function UPF0005; KEGG: sdy:SDY_0945 hypothetical protein; hypothetical protein 2745878..2746537 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159868 YP_003036832.1 CDS ECBD_2625 NC_012947.1 2746628 2746957 D transfers sulfur from TusBCD complex to MnmA; involved in thiouridation of U34 position of some tRNAs; sulfur transfer protein TusE 2746628..2746957 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159869 YP_003036833.1 CDS ECBD_2626 NC_012947.1 2746954 2747232 R catalyzes the hydrolysis of acylphosphate; acylphosphatase complement(2746954..2747232) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159870 YP_003036834.1 CDS ECBD_2627 NC_012947.1 2747342 2748517 D PFAM: protein of unknown function methylase SMART: PUA domain containing protein; KEGG: elf:LF82_2696 UPF0064 protein YccW; hypothetical protein 2747342..2748517 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159871 YP_003036835.1 CDS ECBD_2628 NC_012947.1 2748575 2748892 D TIGRFAM: hemimethylated DNA binding protein; PFAM: Hemimethylated DNA-binding region; KEGG: ssn:SSON_0971 hypothetical protein; heat shock protein HspQ 2748575..2748892 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159872 YP_003036836.1 CDS ECBD_2629 NC_012947.1 2748937 2749350 R PFAM: CoA-binding protein; KEGG: sbc:SbBS512_E2350 CoA-binding protein; CoA-binding protein complement(2748937..2749350) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159873 YP_003036837.1 CDS ECBD_2630 NC_012947.1 2749523 2750185 D KEGG: ssn:SSON_0968 hypothetical protein; hypothetical protein 2749523..2750185 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159874 YP_003036838.1 CDS mgsA NC_012947.1 2750281 2750739 D catalyzes the formation of methylglyoxal from glycerone phosphate; methylglyoxal synthase 2750281..2750739 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159875 YP_003036839.1 CDS helD NC_012947.1 2750771 2752825 R catalyzes the ATP-dependent unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand; DNA helicase IV complement(2750771..2752825) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159876 YP_003036840.1 CDS ECBD_2633 NC_012947.1 2752948 2753394 D PFAM: protein of unknown function DUF307; KEGG: sbc:SbBS512_E2354 hypothetical protein; hypothetical protein 2752948..2753394 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159877 YP_003036841.1 CDS ECBD_2634 NC_012947.1 2753404 2755566 D TIGRFAM: hypothetical protein; integral membrane protein, YccS/YhfK family; PFAM: protein of unknown function DUF893 YccS/YhfK; KEGG: ssn:SSON_0964 hypothetical protein; hypothetical protein 2753404..2755566 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159878 YP_003036842.1 CDS ECBD_2635 NC_012947.1 2755529 2756158 R PFAM: TfoX domain protein; KEGG: ssn:SSON_0963 hypothetical protein; regulator of competence-specific genes complement(2755529..2756158) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159879 YP_003036843.1 CDS ECBD_2636 NC_012947.1 2756377 2756886 D TIGRFAM: cell division inhibitor SulA; PFAM: cell division inhibitor SulA; KEGG: ssn:SSON_0962 SOS cell division inhibitor; SOS cell division inhibitor 2756377..2756886 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159880 YP_003036844.1 CDS ECBD_2637 NC_012947.1 2756870 2757088 R hypothetical protein complement(2756870..2757088) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159881 YP_003036845.1 CDS ECBD_2638 NC_012947.1 2757243 2758283 D OmpA is believed to be a porin, involved in diffusion of nonspecific small solutes across the outer membrane. It is the most abundant integral protein of the outer membrane of E. coli, and it is known to play a role as a phage receptor, a mediator of F-factor dependent conjugation, and in maintaining the structural shape of the outer membrane; 3a; II*; G; d; outer membrane protein A 2757243..2758283 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159882 YP_003036846.1 CDS ECBD_2639 NC_012947.1 2758359 2758811 R PFAM: protein of unknown function DUF1047; KEGG: sdy:SDY_0929 hypothetical protein; hypothetical protein complement(2758359..2758811) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159883 YP_003036847.1 CDS ECBD_2640 NC_012947.1 2758997 2760757 D KEGG: ssn:SSON_0959 ATP-dependent protease; ATP-dependent protease 2758997..2760757 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159884 YP_003036848.1 CDS ECBD_2641 NC_012947.1 2760826 2761344 D catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to 2,3-decenoyl-ACP or 3,4-decenoyl-ACP; 3-hydroxydecanoyl-(acyl carrier protein) dehydratase 2760826..2761344 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158990 YP_003036849.1 CDS ECBD_2642 NC_012947.1 2761414 2761581 R PFAM: ribosome modulation factor; KEGG: sdy:SDY_0926 ribosome modulation factor; ribosome modulation factor complement(2761414..2761581) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158991 YP_003036850.1 CDS ECBD_2643 NC_012947.1 2761837 2762400 R PFAM: protein of unknown function DUF330; KEGG: sfv:SFV_0961 hypothetical protein; hypothetical protein complement(2761837..2762400) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158992 YP_003036851.1 CDS ECBD_2644 NC_012947.1 2762397 2764037 R PFAM: hypothetical protein; KEGG: sdy:SDY_0924 paraquat-inducible protein B; paraquat-inducible protein B complement(2762397..2764037) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157425 YP_003036852.1 CDS ECBD_2645 NC_012947.1 2764042 2765295 R TIGRFAM: integral membrane protein, PqiA family; PFAM: paraquat-inducible protein A; KEGG: sfv:SFV_0959 paraquat-inducible protein A; integral membrane protein, PqiA family complement(2764042..2765295) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157426 YP_003036853.1 CDS ECBD_2646 NC_012947.1 2765425 2767332 R Uup; in Escherichia coli this cytoplasmic protein was shown to contain ATPase activity; mutations in this gene affect RecA-independent excision of transposons and affects Mu bacteriophage growth; ABC transporter ATPase complement(2765425..2767332) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157427 YP_003036854.1 CDS rlmL NC_012947.1 2767344 2769452 R catalyzes the N2-methyl guanosine modification of the G2445 residue of 23S rRNA; 23S rRNA m(2)G2445 methyltransferase complement(2767344..2769452) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157428 YP_003036855.1 CDS ECBD_2648 NC_012947.1 2769696 2770805 D PFAM: MOSC domain containing protein; MOSC domain protein beta barrel domain protein; ferredoxin; KEGG: sbc:SbBS512_E2371 MOSC domain protein; MOSC domain containing protein 2769696..2770805 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157429 YP_003036856.1 CDS ECBD_2649 NC_012947.1 2770802 2771344 R PFAM: protein of unknown function DUF1379; KEGG: sbc:SbBS512_E2370 hypothetical protein; hypothetical protein complement(2770802..2771344) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157430 YP_003036857.1 CDS ECBD_2650 NC_012947.1 2771518 2772528 R catalyzes the conversion of dihydroorotate to orotate in the pyrimidine biosynthesis pathway; uses a flavin nucleotide as an essential cofactor; class 2 enzymes are monomeric and compared to the class 1 class 2 possess an extended N terminus, which plays a role in the membrane association of the enzyme and provides the binding site for the respiratory quinones that serve as physiological electron acceptors; dihydroorotate dehydrogenase 2 complement(2771518..2772528) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157431 YP_003036858.1 CDS ECBD_2651 NC_012947.1 2772639 2773349 R PFAM: Pili assembly chaperone, N-terminal; Pili assembly chaperone, C-terminal; KEGG: ssn:SSON_0948 hypothetical protein; putativi pili assembly chaperone complement(2772639..2773349) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157432 YP_003036859.1 CDS ECBD_2652 NC_012947.1 2773342 2773857 R PFAM: fimbrial protein; KEGG: ssn:SSON_0947 fimbrial-like protein; fimbrial protein complement(2773342..2773857) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157433 YP_003036860.1 CDS ECBD_2653 NC_012947.1 2773865 2774407 R PFAM: fimbrial protein; KEGG: sbc:SbBS512_E2375 fimbrial protein; fimbrial protein complement(2773865..2774407) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157434 YP_003036861.1 CDS ECBD_2654 NC_012947.1 2774419 2775489 R PFAM: fimbrial protein; KEGG: sbc:SbBS512_E2376 fimbrial protein; fimbrial protein complement(2774419..2775489) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157435 YP_003036862.1 CDS ECBD_2655 NC_012947.1 2775480 2778080 R PFAM: fimbrial biogenesis outer membrane usher protein; KEGG: sfv:SFV_0949 outer membrane protein; fimbrial biogenesis outer membrane usher protein complement(2775480..2778080) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157436 YP_003036863.1 CDS ECBD_2656 NC_012947.1 2778105 2778806 R PFAM: Pili assembly chaperone, N-terminal; Pili assembly chaperone, C-terminal; KEGG: ssn:SSON_0943 chaperone; Pili assembly chaperone, N-terminal complement(2778105..2778806) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157437 YP_003036864.1 CDS ECBD_2657 NC_012947.1 2778889 2779428 R PFAM: fimbrial protein; KEGG: sbc:SbBS512_E2380 fimbrial protein; fimbrial protein complement(2778889..2779428) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157438 YP_003036865.1 CDS ECBD_2658 NC_012947.1 2779784 2780359 D PFAM: NADPH-dependent FMN reductase; NAD(P)H dehydrogenase (quinone); KEGG: sbc:SbBS512_E2381 NADPH-dependent FMN reductase; NAD(P)H-dependent FMN reductase 2779784..2780359 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157439 YP_003036866.1 CDS ECBD_2659 NC_012947.1 2780352 2781311 D part of the ABC type transporter SsuABC for aliphatic sulfonates; with SsuA being the periplasmic subunit SsuB the ATP-binding subunit and SsuC the permease; alkanesulfonate transporter substrate-binding subunit 2780352..2781311 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157440 YP_003036867.1 CDS ECBD_2660 NC_012947.1 2781308 2782453 D catalyzes the release of sulfite from alkanesulfonates; alkanesulfonate monooxygenase 2781308..2782453 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157441 YP_003036868.1 CDS ssuC NC_012947.1 2782464 2783255 D part of the ABC type transporter for alkanesulfonate SsuABC; SsuB the ATP-binding subunit and SsuC the permease; alkanesulfonate transporter permease subunit 2782464..2783255 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157442 YP_003036869.1 CDS ssuB NC_012947.1 2783252 2784019 D part of the ABC type transporter SsuABC for aliphatic sulfonates; with SsuA being the periplasmic substrate-binding subunit, SsuB the ATP-binding subunit and SsuC the permease; aliphatic sulfonates transport ATP-binding subunit 2783252..2784019 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157443 YP_003036870.1 CDS pepN NC_012947.1 2784062 2786674 R TIGRFAM: aminopeptidase N; PFAM: peptidase M1 membrane alanine aminopeptidase; aminopeptidase N complement(2784062..2786674) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157444 YP_003036871.1 CDS ECBD_2664 NC_012947.1 2786940 2788142 D catalyzes the formation of nictonate and 5-phospho-alpha-D-ribose 1-diphosphate from nicotinate D-ribonucleotide and diphosphate; nicotinate phosphoribosyltransferase 2786940..2788142 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157445 YP_003036872.1 CDS asnC NC_012947.1 2788311 2789711 D catalyzes a two-step reaction, first charging an asparagine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; asparaginyl-tRNA synthetase 2788311..2789711 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157446 YP_003036873.1 CDS ECBD_2666 NC_012947.1 2790313 2791401 D outer membrane porin F; 1a; Ia; b; outer membrane protein F 2790313..2791401 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157447 YP_003036874.1 CDS ECBD_2667 NC_012947.1 2791586 2792776 D catalyzes the formation of L-glutamate and an aromatic oxo acid from an aromatic amino acid and 2-oxoglutarate; aromatic amino acid aminotransferase 2791586..2792776 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157448 YP_003036875.1 CDS ECBD_2668 NC_012947.1 2792998 2793645 R PFAM: beta-lactamase; KEGG: sbo:SBO_2218 hypothetical protein; beta-lactamase complement(2792998..2793645) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157449 YP_003036876.1 CDS ECBD_2669 NC_012947.1 2793672 2794220 R PFAM: protein of unknown function DUF882; peptidase M15A; KEGG: sdy:SDY_2331 hypothetical protein; hypothetical protein complement(2793672..2794220) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157450 YP_003036877.1 CDS ECBD_2670 NC_012947.1 2794401 2796248 R PFAM: ErfK/YbiS/YcfS/YnhG family protein; peptidoglycan-binding protein; KEGG: sbo:SBO_2216 hypothetical protein; hypothetical protein complement(2794401..2796248) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157451 YP_003036878.1 CDS mukB NC_012947.1 2796509 2800969 R SMC (structural maintenance of chromosomes) family of proteins; involved in chromosome condensatin and partitioning; forms a homodimer and the C-terminal is essential for DNA-binding activity while the purified N-terminal domain binds FtsZ; mutations result in cell division defects; cell division protein MukB complement(2796509..2800969) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157452 YP_003036879.1 CDS ECBD_2672 NC_012947.1 2800969 2801673 R acts with MukB and MukF to condense the chromosome and allow for segregation during cell division; condesin subunit E complement(2800969..2801673) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157453 YP_003036880.1 CDS ECBD_2673 NC_012947.1 2801654 2802976 R acts with MukB and MukE to condense the chromosome and allow for segregation during cell division; condesin subunit F complement(2801654..2802976) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157454 YP_003036881.1 CDS ECBD_2674 NC_012947.1 2802973 2803758 R involved in cellular zinc and cadmium homeostasis by binding excess metal ions to its cysteine sulfurs and histidine nitrogens; expression of smtA in cyanobacteria is repressed by SmtB at low concentrations of zinc; NO elicits release of Zn ions from SmtA; metallothionein SmtA complement(2802973..2803758) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157455 YP_003036882.1 CDS ECBD_2675 NC_012947.1 2803894 2804673 D PFAM: protein of unknown function DUF218; KEGG: elf:LF82_2680 uncharacterized protein YcbC; hypothetical protein 2803894..2804673 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157456 YP_003036883.1 CDS ECBD_2676 NC_012947.1 2804650 2805543 R KEGG: sbc:SbBS512_E2404 hypothetical protein; hypothetical protein complement(2804650..2805543) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157457 YP_003036884.1 CDS ECBD_2677 NC_012947.1 2805697 2806443 R CMP-2-keto-3-deoxyoctulosonic acid synthetase; catalyzes the formation of CMP-3-deoxy-D-manno-octulosonate from CTP and 3-deoxy-D-manno-octulosonate which is incorporated into LPS; 3-deoxy-manno-octulosonate cytidylyltransferase complement(2805697..2806443) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157458 YP_003036885.1 CDS ECBD_2678 NC_012947.1 2806440 2806622 R PFAM: protein of unknown function DUF343; KEGG: ssn:SSON_0919 hypothetical protein; hypothetical protein complement(2806440..2806622) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157459 YP_003036886.1 CDS ECBD_2679 NC_012947.1 2806674 2807906 R PFAM: protein of unknown function DUF1006; KEGG: sdy:SDY_2342 hypothetical protein; hypothetical protein complement(2806674..2807906) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157460 YP_003036887.1 CDS lpxK NC_012947.1 2807943 2808929 R transfers the gamma-phosphate of ATP to the 4' position of a tetraacyldisaccharide 1-phosphate intermediate to form tetraacyldisaccharide 1,4'-bis-phosphate; tetraacyldisaccharide 4'-kinase complement(2807943..2808929) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157461 YP_003036888.1 CDS ECBD_2681 NC_012947.1 2808926 2810674 R involved in the transport of lipid A across the inner membrane; lipid transporter ATP-binding/permease complement(2808926..2810674) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157462 YP_003036889.1 CDS ECBD_2682 NC_012947.1 2810711 2812975 R TIGRFAM: DNA internalization-related competence protein ComEC/Rec2; ComEC/Rec2-related protein; PFAM: ComEC/Rec2-related protein; beta-lactamase; KEGG: sdy:SDY_2345 hypothetical protein; hypothetical protein complement(2810711..2812975) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157463 YP_003036890.1 CDS ihfB NC_012947.1 2813183 2813467 R This protein is one of the two subunits of integration host factor, a specific DNA-binding protein that functions in genetic recombination as well as in transcriptional and translational control; integration host factor subunit beta complement(2813183..2813467) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157464 YP_003036891.1 CDS rpsA NC_012947.1 2813627 2815300 R in Escherichia coli this protein is involved in binding to the leader sequence of mRNAs and is itself bound to the 30S subunit; autoregulates expression via a C-terminal domain; in most gram negative organisms this protein is composed of 6 repeats of the S1 domain while in gram positive there are 4 repeats; the S1 nucleic acid-binding domain is found associated with other proteins; 30S ribosomal protein S1 complement(2813627..2815300) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157465 YP_003036892.1 CDS cmk NC_012947.1 2815411 2816094 R Catalyzes the formation of (d)CDP from ATP and (d)CMP; cytidylate kinase complement(2815411..2816094) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157466 YP_003036893.1 CDS ECBD_2686 NC_012947.1 2816267 2817031 R PFAM: peptidase M48 Ste24p; KEGG: ssn:SSON_0911 heat shock protein; peptidase M48 Ste24p complement(2816267..2817031) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157467 YP_003036894.1 CDS ECBD_2687 NC_012947.1 2817200 2818483 R catalyzes the formation of 5-O-(1-carboxyvinyl)-3-phosphoshikimate from phosphoenolpyruvate and 3-phosphoshikimate in tryptophan biosynthesis; 3-phosphoshikimate 1-carboxyvinyltransferase complement(2817200..2818483) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157468 YP_003036895.1 CDS ECBD_2688 NC_012947.1 2818554 2819642 R catalyzes the formation of 3-phosphonooxypyruvate and glutamate from O-phospho-L-serine and 2-oxoglutarate; required both in major phosphorylated pathway of serine biosynthesis and in the biosynthesis of pyridoxine; phosphoserine aminotransferase complement(2818554..2819642) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157469 YP_003036896.1 CDS ECBD_2689 NC_012947.1 2819841 2820533 R PFAM: protein of unknown function DUF421; KEGG: ssn:SSON_0907 hypothetical protein; hypothetical protein complement(2819841..2820533) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157470 YP_003036897.1 CDS ECBD_2690 NC_012947.1 2820663 2822423 D PFAM: protein of unknown function DUF181; KEGG: ssn:SSON_0906 hypothetical protein; hypothetical protein 2820663..2822423 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157471 YP_003036898.1 CDS ECBD_2691 NC_012947.1 2822828 2823685 D TIGRFAM: formate/nitrite transporter; PFAM: formate/nitrite transporter; KEGG: ssn:SSON_0905 formate transporter; formate transporter 2822828..2823685 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157472 YP_003036899.1 CDS ECBD_2692 NC_012947.1 2823740 2826022 D KEGG: ssn:SSON_0904 formate acetyltransferase 1; TIGRFAM: formate acetyltransferase; PFAM: pyruvate formate-lyase PFL; formate C-acetyltransferase glycine radical; formate acetyltransferase 2823740..2826022 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157473 YP_003036900.1 CDS pflA NC_012947.1 2826214 2826954 D activates pyruvate formate-lyase 1 under anaerobic conditions; pyruvate formate lyase-activating enzyme 1 2826214..2826954 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157474 YP_003036901.1 CDS ECBD_2694 NC_012947.1 2827036 2827626 R PFAM: NAD(P)H dehydrogenase (quinone); NADPH-dependent FMN reductase; KEGG: sbo:SBO_2185 hypothetical protein; hypothetical protein complement(2827036..2827626) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157475 YP_003036902.1 CDS ECBD_2695 NC_012947.1 2827726 2828634 D PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: sbo:SBO_2184 transcriptional regulator LysR-type; LysR family transcriptional regulator 2827726..2828634 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157476 YP_003036903.1 CDS ECBD_2696 NC_012947.1 2828635 2830065 R PFAM: amino acid permease-associated region; amino acid permease-associated region complement(2828635..2830065) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157477 YP_003036904.1 CDS ECBD_2697 NC_012947.1 2830275 2831423 R PFAM: major facilitator superfamily MFS_1; KEGG: sdy:SDY_2363 MFS family transporter protein; MFS family transporter protein complement(2830275..2831423) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157478 YP_003036905.1 CDS ECBD_2698 NC_012947.1 2831737 2832363 D PFAM: isochorismatase hydrolase; KEGG: ssn:SSON_0898 hypothetical protein; isochorismatase hydrolase 2831737..2832363 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157479 YP_003036906.1 CDS ECBD_2699 NC_012947.1 2832398 2833261 R PFAM: DMSO reductase anchor subunit (DmsC); KEGG: sbc:SbBS512_E2432 anaerobic dimethyl sulfoxide reductase, C subunit; DMSO reductase anchor subunit (DmsC) complement(2832398..2833261) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157480 YP_003036907.1 CDS ECBD_2700 NC_012947.1 2833263 2833880 R TIGRFAM: dimethylsulfoxide reductase, chain B; PFAM: 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: ssn:SSON_0896 anaerobic dimethyl sulfoxide reductase subunit B; dimethylsulfoxide reductase, chain B complement(2833263..2833880) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157481 YP_003036908.1 CDS ECBD_2701 NC_012947.1 2833891 2836335 R TIGRFAM: anaerobic dimethyl sulfoxide reductase, A subunit, DmsA/YnfE family; PFAM: molybdopterin oxidoreductase; molybdopterin oxidoreductase Fe4S4 region; molydopterin dinucleotide-binding region; anaerobic dimethyl sulfoxide reductase, subunit alpha, DmsA/YnfE family complement(2833891..2836335) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157482 YP_003036909.1 CDS ECBD_2702 NC_012947.1 2836574 2837866 R catalyzes a two-step reaction, first charging a serine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; seryl-tRNA synthetase complement(2836574..2837866) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157483 YP_003036910.1 CDS ECBD_2703 NC_012947.1 2837957 2839300 R PFAM: ATPase AAA; ATPase associated with various cellular activities AAA_5; SMART: ATPase AAA; KEGG: sdy:SDY_2369 recombination factor protein RarA; recombination factor protein RarA complement(2837957..2839300) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157484 YP_003036911.1 CDS lolA NC_012947.1 2839311 2839922 R participates with LolB in the incorporation of lipoprotein into the outer membrane; outer-membrane lipoprotein carrier protein complement(2839311..2839922) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157485 YP_003036912.1 CDS ECBD_2705 NC_012947.1 2840077 2844105 R DNA-binding membrane protein required for chromosome resolution and partitioning; DNA translocase FtsK complement(2840077..2844105) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157486 YP_003036913.1 CDS ECBD_2706 NC_012947.1 2844240 2844734 R mediates a global response to leucine; acts as a regulator for several genes involved in the high-affinity branched-chain amino acid transporter; leucine-responsive transcriptional regulator complement(2844240..2844734) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157487 YP_003036914.1 CDS ECBD_2707 NC_012947.1 2845279 2846244 D catalyzes the transfer of electrons from NADPH to thioredoxin; FAD/NAD(P) binding; thioredoxin reductase 2845279..2846244 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157488 YP_003036915.1 CDS ECBD_2708 NC_012947.1 2846367 2848133 D in Escherichia coli the CydCD ABC transporter exports cysteine and glutathione into the periplasm in order to maintain redox balance; important for cytochrome bd and c; cysteine/glutathione ABC transporter membrane/ATP-binding component 2846367..2848133 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157489 YP_003036916.1 CDS ECBD_2709 NC_012947.1 2848134 2849855 D in Escherichia coli the CydCD ABC transporter exports cysteine and glutathione into the periplasm in order to maintain redox balance; important for cytochrome bd and c; cysteine/glutathione ABC transporter membrane/ATP-binding component 2848134..2849855 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157490 YP_003036917.1 CDS aat NC_012947.1 2849897 2850601 D leucyltransferase; phenylalanyltransferse; functions in the N-end rule pathway; transfers Leu, Phe, Met, from aminoacyl-tRNAs to N-terminal of proteins with Arg or Lys; leucyl/phenylalanyl-tRNA--protein transferase 2849897..2850601 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157491 YP_003036918.1 CDS infA NC_012947.1 2850886 2851104 D stimulates the activities of the other two initiation factors, IF-2 and IF-3; translation initiation factor IF-1 2850886..2851104 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157492 YP_003036919.1 CDS clpA NC_012947.1 2852142 2854418 R ATPase and specificity subunit of the ClpA-ClpP ATP dependent serine protease; directs protease to specific substrates; ATP-dependent Clp protease ATP-binding subunit complement(2852142..2854418) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157494 YP_003036920.1 CDS clpS NC_012947.1 2854449 2854769 R involved in the modulation of the specificity of the ClpAP-mediated ATP-dependent protein degradation; binds to the N-terminal domain of the chaperone ClpA; ATP-dependent Clp protease adaptor protein ClpS complement(2854449..2854769) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157495 YP_003036921.1 CDS ECBD_2714 NC_012947.1 2855092 2855316 D inhibits DNA replication at both initiation and elongation steps; stationary phase and starvation inducible; stationary phase/starvation inducible regulatory protein CspD 2855092..2855316 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157496 YP_003036922.1 CDS ECBD_2715 NC_012947.1 2855389 2857335 R with MacA is involved in the export of macrolide; macrolide transporter ATP-binding /permease complement(2855389..2857335) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157497 YP_003036923.1 CDS ECBD_2716 NC_012947.1 2857332 2858447 R confers macrolide resistance via active drug efflux; macrolide transporter subunit MacA complement(2857332..2858447) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157498 YP_003036924.1 CDS ECBD_2717 NC_012947.1 2858562 2859554 D PFAM: protein of unknown function DUF535; KEGG: ssn:SSON_0864 enzyme; hypothetical protein 2858562..2859554 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157499 YP_003036925.1 CDS ECBD_2718 NC_012947.1 2859551 2861209 R KEGG: sfx:S0876 hypothetical protein; hypothetical protein complement(2859551..2861209) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157500 YP_003036926.1 CDS ECBD_2719 NC_012947.1 2861634 2862329 D porin involved in osmoregulation allowing water to move into and out of the cell in response to osmotic pressure; aquaporin Z 2861634..2862329 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157501 YP_003036927.1 CDS ECBD_2720 NC_012947.1 2862824 2863723 D PFAM: protein of unknown function DUF340 membrane; KEGG: sbo:SBO_0807 surface protein; hypothetical protein 2862824..2863723 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157502 YP_003036928.1 CDS ECBD_2721 NC_012947.1 2863867 2865519 D catalyzes the reduction of hydroxylamine to ammonia and water; hydroxylamine reductase 2863867..2865519 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157503 YP_003036929.1 CDS ECBD_2722 NC_012947.1 2865531 2866499 D PFAM: ferredoxin; oxidoreductase FAD/NAD(P)-binding domain protein; oxidoreductase FAD-binding domain protein; KEGG: sbc:SbBS512_E2459 NADH oxidoreductase hcr; HCP oxidoreductase, NADH-dependent 2865531..2866499 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157586 YP_003036930.1 CDS ECBD_2723 NC_012947.1 2866632 2868350 D catalyzes the formation of acetate from pyruvate; pyruvate dehydrogenase 2866632..2868350 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157587 YP_003036931.1 CDS ECBD_2724 NC_012947.1 2868387 2869388 D low- specificity; catalyzes the formation of acetaldehyde and glycine from L-threonine; acts on L-threonine, L-allo-threonine, L-threo-phenylserine, and L-erythro-phenylserine; L-threonine aldolase 2868387..2869388 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157588 YP_003036932.1 CDS ECBD_2725 NC_012947.1 2869399 2870829 D PFAM: NAD-dependent epimerase/dehydratase; NmrA family protein; 3-beta hydroxysteroid dehydrogenase/isomerase; KEGG: sbc:SbBS512_E2462 NAD dependent epimerase/dehydratase family protein; NAD-dependent epimerase/dehydratase 2869399..2870829 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157589 YP_003036933.1 CDS ECBD_2726 NC_012947.1 2870892 2871941 D PFAM: NAD-dependent epimerase/dehydratase; 3-beta hydroxysteroid dehydrogenase/isomerase; Male sterility domain; KEGG: ssn:SSON_0854 nucleotide di-P-sugar epimerase or dehydratase; NAD-dependent epimerase/dehydratase 2870892..2871941 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157590 YP_003036934.1 CDS ECBD_2727 NC_012947.1 2871938 2872768 R KEGG: sdy:SDY_2396 regulator; PFAM: N-acetylmuramoyl-L-alanine amidase family 2; SMART: N-acetylmuramoyl-L-alanine amidase family 2; N-acetylmuramyl-L-alanine amidase, negative regulator of AmpC, AmpD complement(2871938..2872768) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157591 YP_003036935.1 CDS ECBD_2728 NC_012947.1 2872765 2873088 R PFAM: protein of unknown function DUF74; KEGG: sdy:SDY_2397 hypothetical protein; hypothetical protein complement(2872765..2873088) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157592 YP_003036936.1 CDS ECBD_2729 NC_012947.1 2873214 2873729 D induced during stationary phase and by acivicin (a glutamine analog); regulated by Lrp and RpoS; lipoprotein 2873214..2873729 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157593 YP_003036937.1 CDS artP NC_012947.1 2873947 2874675 D With ArtMQJI transports arginine across the inner membrane; arginine transporter ATP-binding subunit 2873947..2874675 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157594 YP_003036938.1 CDS ECBD_2731 NC_012947.1 2874693 2875424 D TIGRFAM: cationic amino acid ABC transporter periplasmic binding protein; KEGG: ssn:SSON_0848 arginine 3rd transporter periplasmic binding protein; cationic amino acid ABC transporter periplasmic binding protein 2874693..2875424 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157595 YP_003036939.1 CDS ECBD_2732 NC_012947.1 2875431 2876147 D with ArtPMJI transports arginine across the inner membrane; arginine transporter permease subunit ArtQ 2875431..2876147 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157596 YP_003036940.1 CDS artM NC_012947.1 2876147 2876815 D with ArtPQJI acts to transport arginine across the inner membrane; arginine transporter permease subunit ArtM 2876147..2876815 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157597 YP_003036941.1 CDS ECBD_2734 NC_012947.1 2877106 2877837 D TIGRFAM: cationic amino acid ABC transporter periplasmic binding protein; KEGG: sbo:SBO_0794 arginine 3rd transporter periplasmic binding protein; cationic amino acid ABC transporter periplasmic binding protein 2877106..2877837 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157598 YP_003036942.1 CDS rumB NC_012947.1 2878036 2879163 R RNA uridine methyltransferase B; catalyzes the formation of 5-methyl-uridine at position 747 in 23S rRNA; 23S rRNA methyluridine methyltransferase complement(2878036..2879163) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157599 YP_003036943.1 CDS ECBD_2736 NC_012947.1 2879204 2879692 R KEGG: sdy:SDY_0737 hypothetical protein; hypothetical protein complement(2879204..2879692) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157600 YP_003036944.1 CDS ECBD_2737 NC_012947.1 2879752 2880597 R KEGG: sdy:SDY_0738 putrescine transporter subunit: membrane component of ABC superfamily; putrescine transporter subunit: membrane component of ABC superfamily complement(2879752..2880597) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157601 YP_003036945.1 CDS ECBD_2738 NC_012947.1 2880594 2881547 R KEGG: efe:EFER_0999 putrescine transporter subunit: membrane component of ABC superfamily; putrescine transporter subunit: membrane component of ABC superfamily complement(2880594..2881547) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157602 YP_003036946.1 CDS potG NC_012947.1 2881557 2882690 R part of the PotFGHI ATP-dependent putrescine transporter; putrescine transporter ATP-binding subunit complement(2881557..2882690) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157603 YP_003036947.1 CDS ECBD_2740 NC_012947.1 2882785 2883897 R PFAM: extracellular solute-binding protein family 1; KEGG: sbc:SbBS512_E2477 putrescine ABC transporter periplasmic putrescine-binding protein; putrescine transporter subunit: periplasmic-binding component of ABC superfamily complement(2882785..2883897) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157604 YP_003036948.1 CDS ECBD_2741 NC_012947.1 2884248 2884724 R KEGG: ssn:SSON_0838 sensory transduction regulator; sensory transduction regulator complement(2884248..2884724) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157605 YP_003036949.1 CDS ECBD_2742 NC_012947.1 2884812 2885714 R responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6; ribosomal protein S6 modification protein complement(2884812..2885714) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157606 YP_003036950.1 CDS ECBD_2743 NC_012947.1 2885775 2886497 R NADPH-dependent; oxygen-insensitive; catalyzes the reduction of nitroaromatic compounds; nitroreductase A complement(2885775..2886497) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157607 YP_003036951.1 CDS ECBD_2744 NC_012947.1 2886481 2886768 R YbjC; located in the same operon as the nfsA gene; member of the soxRS regulon which mediates in part the increased levels of superoxide in response to oxidative stress; induced by paraquat; regulated by SoxS; unknown function; hypothetical protein complement(2886481..2886768) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157608 YP_003036952.1 CDS grxA NC_012947.1 2886928 2887185 D functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase; also involved in reducing some disulfides in a coupled system with glutathione reductase; glutaredoxin 2886928..2887185 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157609 YP_003036953.1 CDS ECBD_2746 NC_012947.1 2887215 2887592 R KEGG: ssn:SSON_0833 hypothetical protein; hypothetical protein complement(2887215..2887592) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157610 YP_003036954.1 CDS ECBD_2747 NC_012947.1 2887862 2889547 D TIGRFAM: YidE/YbjL duplication; PFAM: YidE/YbjL duplication domain protein; TrkA-C domain protein; KEGG: sbc:SbBS512_E2485 transporter; hypothetical protein 2887862..2889547 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157611 YP_003036955.1 CDS ECBD_2748 NC_012947.1 2889783 2890001 R PFAM: transcriptional activator Ogr/delta; KEGG: sbc:SbBS512_E2486 phage transcriptional activator, Ogr/delta; transcriptional activator Ogr/delta complement(2889783..2890001) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157612 YP_003036956.1 CDS ECBD_2750 NC_012947.1 2890457 2890732 R PFAM: Resolvase domain; KEGG: stt:t2667 DNA-invertase; resolvase complement(2890457..2890732) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157614 YP_003036957.1 CDS ECBD_2751 NC_012947.1 2890760 2891302 D PFAM: Tail Collar domain protein; KEGG: stt:t1351 bacteriophage tail fiber protein; Tail Collar domain protein 2890760..2891302 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157615 YP_003036958.1 CDS ECBD_2752 NC_012947.1 2891302 2891904 D PFAM: tail assembly chaperone gp38; KEGG: stt:t1349 bacteriophage tail fiber assembly protein; tail assembly chaperone gp38 2891302..2891904 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157616 YP_003036959.1 CDS ECBD_2753 NC_012947.1 2891876 2892313 R PFAM: tail assembly chaperone gp38; KEGG: stt:t3435 hypothetical protein; tail assembly chaperone gp38 complement(2891876..2892313) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157617 YP_003036960.1 CDS ECBD_2754 NC_012947.1 2892315 2893937 R KEGG: elf:LF82_p389 variable tail fibre protein; phage-like tail fiber protein complement(2892315..2893937) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157618 YP_003036961.1 CDS ECBD_2755 NC_012947.1 2893934 2894539 R TIGRFAM: phage tail protein I; KEGG: elf:LF82_p390 phage tail protein; phage tail protein I complement(2893934..2894539) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157619 YP_003036962.1 CDS ECBD_2756 NC_012947.1 2894532 2895440 R PFAM: Baseplate J family protein; KEGG: elf:LF82_p391 phage baseplate assembly protein; Baseplate J family protein complement(2894532..2895440) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157620 YP_003036963.1 CDS ECBD_2757 NC_012947.1 2895427 2895786 R PFAM: GPW/gp25 family protein; KEGG: elf:LF82_p392 phage baseplate assembly protein; GPW/gp25 family protein complement(2895427..2895786) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157621 YP_003036964.1 CDS ECBD_2758 NC_012947.1 2895783 2896361 R TIGRFAM: phage baseplate assembly protein V; PFAM: baseplate assembly protein V; KEGG: stt:t3429 phage baseplate assembly protein; phage baseplate assembly protein V complement(2895783..2896361) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157622 YP_003036965.1 CDS ECBD_2759 NC_012947.1 2896465 2897271 D KEGG: esa:ESA_02491 hypothetical protein; hypothetical protein 2896465..2897271 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157623 YP_003036966.1 CDS ECBD_2760 NC_012947.1 2897213 2897659 R TIGRFAM: phage virion morphogenesis protein; PFAM: virion morphogenesis ( tail completion) protein; KEGG: stt:t3428 phage tail protein; phage virion morphogenesis protein complement(2897213..2897659) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157624 YP_003036967.1 CDS ECBD_2761 NC_012947.1 2897652 2898083 R PFAM: P2 phage tail completion R family protein; KEGG: sed:SeD_A3040 phage tail completion protein R; P2 phage tail completion R family protein complement(2897652..2898083) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157625 YP_003036968.1 CDS ECBD_2762 NC_012947.1 2898179 2898607 R KEGG: elf:LF82_p396 regulatory protein; regulatory protein complement(2898179..2898607) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157626 YP_003036969.1 CDS ECBD_2763 NC_012947.1 2898744 2899496 R PFAM: capsid scaffolding; KEGG: sbo:SBO_0759 capsid scaffolding protein; capsid scaffolding complement(2898744..2899496) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157627 YP_003036970.1 CDS ECBD_2764 NC_012947.1 2899639 2901405 D PFAM: protein of unknown function DUF264; terminase ATPase; KEGG: stt:t3417 terminase, ATPase; hypothetical protein 2899639..2901405 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157628 YP_003036971.1 CDS ECBD_2765 NC_012947.1 2901405 2902430 D TIGRFAM: phage portal protein, PBSX family; PFAM: portal protein; KEGG: stt:t3416 capsid portal protein; phage portal protein, PBSX family 2901405..2902430 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157629 YP_003036972.1 CDS ECBD_2766 NC_012947.1 2902448 2903410 R PFAM: RNA-directed DNA polymerase (Reverse transcriptase); KEGG: har:HEAR0006 hypothetical protein; RNA-directed DNA polymerase complement(2902448..2903410) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157630 YP_003036973.1 CDS ECBD_2767 NC_012947.1 2903573 2904496 R KEGG: har:HEAR0007 hypothetical protein; hypothetical protein complement(2903573..2904496) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157631 YP_003036974.1 CDS ECBD_2768 NC_012947.1 2904969 2905304 D hypothetical protein 2904969..2905304 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157632 YP_003036975.1 CDS ECBD_2769 NC_012947.1 2905497 2905730 R PFAM: DinI family protein; KEGG: stm:STM2727 hypothetical protein; DinI family protein complement(2905497..2905730) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157633 YP_003036976.1 CDS ECBD_2770 NC_012947.1 2905741 2905929 R KEGG: stt:t3411 hypothetical protein; hypothetical protein complement(2905741..2905929) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157634 YP_003036977.1 CDS ECBD_2771 NC_012947.1 2906082 2907527 R PFAM: regulatory CII family protein; replication gene A; KEGG: elf:LF82_p407 replication protein for prophage; regulatory CII family protein complement(2906082..2907527) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157635 YP_003036978.1 CDS ECBD_2772 NC_012947.1 2907560 2907781 R KEGG: sbo:SBO_0749 hypothetical protein; hypothetical protein complement(2907560..2907781) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157636 YP_003036979.1 CDS ECBD_2773 NC_012947.1 2907907 2908476 D PFAM: CI repressor; KEGG: sbo:SBO_0748 repressor protein; CI repressor 2907907..2908476 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157637 YP_003036980.1 CDS ECBD_2774 NC_012947.1 2908492 2908683 D KEGG: sdy:SDY_1763 hypothetical protein; hypothetical protein 2908492..2908683 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157638 YP_003036981.1 CDS ECBD_2775 NC_012947.1 2908870 2909886 D PFAM: integrase; KEGG: stt:t3400 bacteriophage integrase; integrase 2908870..2909886 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157639 YP_003036982.1 CDS ECBD_2776 NC_012947.1 2909995 2910327 D KEGG: stt:t3767 hypothetical protein; hypothetical protein 2909995..2910327 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157640 YP_003036983.1 CDS ECBD_2777 NC_012947.1 2910507 2911043 R PFAM: regulatory protein TetR; KEGG: sbc:SbBS512_E2499 TetR family transcriptional regulator; TetR family transcriptional regulator complement(2910507..2911043) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157641 YP_003036984.1 CDS ECBD_2778 NC_012947.1 2911127 2912335 D PFAM: major facilitator superfamily MFS_1; KEGG: elf:LF82_2659 inner membrane protein YbjJ; major facilitator superfamily MFS_1 2911127..2912335 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157642 YP_003036985.1 CDS ECBD_2779 NC_012947.1 2912335 2913150 D TIGRFAM: Cof-like hydrolase; HAD-superfamily hydrolase, subfamily IIB; PFAM: haloacid dehalogenase type 3; haloacid dehalogenase; sucrose-6F-phosphate phosphohydrolase; KEGG: sfv:SFV_0829 hypothetical protein; Cof-like hydrolase 2912335..2913150 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157643 YP_003036986.1 CDS ECBD_2780 NC_012947.1 2913242 2913520 D KEGG: sfv:SFV_0828 hypothetical protein; hypothetical protein 2913242..2913520 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157644 YP_003036987.1 CDS ECBD_2781 NC_012947.1 2913561 2914793 R PFAM: major facilitator superfamily MFS_1; major facilitator superfamily MFS_1 complement(2913561..2914793) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157645 YP_003036988.1 CDS ECBD_2782 NC_012947.1 2915078 2915674 D KEGG: ssn:SSON_0826 undecaprenyl pyrophosphate phosphatase; PFAM: phosphoesterase PA-phosphatase related; SMART: phosphoesterase PA-phosphatase related; UDP pyrophosphate phosphatase 2915078..2915674 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157646 YP_003036989.1 CDS ECBD_2783 NC_012947.1 2915732 2916490 D PFAM: regulatory protein DeoR; SMART: regulatory protein DeoR; DeoR family transcriptional regulator 2915732..2916490 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157647 YP_003036990.1 CDS ECBD_2784 NC_012947.1 2916537 2917739 R penicillin-binding protein 6a; removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors; one of four, DD-carboxypeptidase low-molecular weight penicillin-binding proteins that remove terminal D-alanine from pentapeptide side chains; D-alanyl-D-alanine carboxypeptidase fraction C complement(2916537..2917739) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157648 YP_003036991.1 CDS ECBD_2785 NC_012947.1 2917986 2918612 D PFAM: glutathione S-transferase domain; KEGG: sfx:S0831 transferase; glutathione S-transferase domain protein 2917986..2918612 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157649 YP_003036992.1 CDS ECBD_2786 NC_012947.1 2918609 2919724 R PFAM: glucose sorbosone dehydrogenase; KEGG: sbo:SBO_0733 dehydrogenase; glucose sorbosone dehydrogenase complement(2918609..2919724) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157650 YP_003036993.1 CDS bssR NC_012947.1 2919835 2920218 R BssS; regulator of biofilm through signal secretion; disruption of this gene in Escherichia coli led to effects on biofilm formation, alteration in expression of a number of genes and mutations in bssS led to defects in indole transport and autoinducer-2 uptake and processing; biofilm formation regulatory protein BssR complement(2919835..2920218) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157651 YP_003036994.1 CDS rimO NC_012947.1 2920431 2921756 D catalyzes the methylthiolation of an aspartic acid residue of the S12 protein of the 30S ribosomal subunit; ribosomal protein S12 methylthiotransferase 2920431..2921756 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157652 YP_003036995.1 CDS ECBD_2789 NC_012947.1 2921803 2923131 R KEGG: sbo:SBO_0726 hypothetical protein; TIGRFAM: diguanylate cyclase; PFAM: GGDEF domain containing protein; SMART: GGDEF domain containing protein; diguanylate cyclase complement(2921803..2923131) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157653 YP_003036996.1 CDS ECBD_2791 NC_012947.1 2925665 2926576 R PFAM: binding-protein-dependent transporters inner membrane component; KEGG: sbc:SbBS512_E2516 glutathione ABC transporter permease GsiD; binding-protein-dependent transporters inner membrane component complement(2925665..2926576) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157655 YP_003036997.1 CDS ECBD_2792 NC_012947.1 2926579 2927499 R PFAM: binding-protein-dependent transporters inner membrane component; KEGG: sdy:SDY_0756 transporter permease protein; binding-protein-dependent transporters inner membrane component complement(2926579..2927499) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157656 YP_003036998.1 CDS ECBD_2793 NC_012947.1 2927517 2929055 R PFAM: extracellular solute-binding protein family 5; KEGG: ssn:SSON_0812 transporter; extracellular solute-binding protein family 5 complement(2927517..2929055) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157657 YP_003036999.1 CDS ECBD_2794 NC_012947.1 2929075 2930946 R with GsiBCD is involved in glutathione import; GsiA contains 2 ATP-binding domains; glutathione transporter ATP-binding protein complement(2929075..2930946) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157658 YP_003037000.1 CDS ECBD_2795 NC_012947.1 2930933 2931898 R PFAM: peptidase T2 asparaginase 2; KEGG: ssn:SSON_0810 L-asparaginase; L-asparaginase complement(2930933..2931898) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157659 YP_003037001.1 CDS ECBD_2796 NC_012947.1 2932102 2933337 D is involved in the formation of active molybdenum cofactor and the chelation of molybdenum; molybdopterin biosynthesis protein MoeA 2932102..2933337 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157660 YP_003037002.1 CDS ECBD_2797 NC_012947.1 2933337 2934086 D ATP-dependent adenylate transferase, transfers adenyl moiety to the MoeD subunit of molybdopterin synthase; molybdopterin biosynthesis protein MoeB 2933337..2934086 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157661 YP_003037003.1 CDS ECBD_2798 NC_012947.1 2934162 2934824 R similar to transaldolase from Escherichia coli; many organisms have multiple copies; fructose-6-phosphate aldolase complement(2934162..2934824) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157662 YP_003037004.1 CDS ECBD_2799 NC_012947.1 2934955 2935854 D KEGG: sfv:SFV_0807 pyruvate formate-lyase 2 activating enzyme; TIGRFAM: glycyl-radical enzyme activating protein family; PFAM: radical SAM protein; glycyl-radical enzyme activating protein family 2934955..2935854 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157663 YP_003037005.1 CDS ECBD_2800 NC_012947.1 2935860 2938292 D KEGG: elf:LF82_2650 formate acetyltransferase 3; TIGRFAM: pyruvate formate-lyase; PFAM: pyruvate formate-lyase PFL; formate C-acetyltransferase glycine radical; pyruvate formate-lyase 2935860..2938292 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157504 YP_003037006.1 CDS ECBD_2801 NC_012947.1 2938438 2939253 D TIGRFAM: Cof-like hydrolase; HAD-superfamily hydrolase, subfamily IIB; PFAM: haloacid dehalogenase type 3; sucrose-6F-phosphate phosphohydrolase; Haloacid dehalogenase domain protein hydrolase; KEGG: sfv:SFV_0805 hypothetical protein; Cof-like hydrolase 2938438..2939253 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158405 YP_003037007.1 CDS ECBD_2802 NC_012947.1 2939405 2940670 D PFAM: protein of unknown function DUF1479; KEGG: sbo:SBO_0711 hypothetical protein; hypothetical protein 2939405..2940670 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158324 YP_003037008.1 CDS ECBD_2803 NC_012947.1 2940838 2942430 R PFAM: ABC transporter; SMART: ATPase AAA; ABC transporter complement(2940838..2942430) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158325 YP_003037009.1 CDS ECBD_2804 NC_012947.1 2942649 2943569 D PFAM: ErfK/YbiS/YcfS/YnhG family protein; KEGG: sdy:SDY_0777 hypothetical protein; hypothetical protein 2942649..2943569 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158326 YP_003037010.1 CDS ECBD_2805 NC_012947.1 2943628 2944746 R PFAM: citrate transporter; KEGG: ssn:SSON_0797 hypothetical protein; citrate transporter complement(2943628..2944746) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158327 YP_003037011.1 CDS ECBD_2806 NC_012947.1 2944743 2945210 R transcriptional regulator that represses the manganese transporter MntH when manganese is present; manganese transport regulator MntR complement(2944743..2945210) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158328 YP_003037012.1 CDS ECBD_2807 NC_012947.1 2945396 2945524 D KEGG: sbc:SbBS512_E2532 hypothetical protein; hypothetical protein 2945396..2945524 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158329 YP_003037013.1 CDS ECBD_2808 NC_012947.1 2945796 2947379 D PFAM: sulfatase; KEGG: sbc:SbBS512_E2533 sulfatase family protein; sulfatase 2945796..2947379 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158330 YP_003037014.1 CDS ompX NC_012947.1 2947428 2947943 R OmpX; involved in cell adhesion; forms an eight-stranded antiparallel beta-barrel that protrudes from the cell surface; mutations in the gene increase cell-surface contact in fimbriated strains but decrease contact in nonfimbriated strains; outer membrane protein X complement(2947428..2947943) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158331 YP_003037015.1 CDS ECBD_2810 NC_012947.1 2948296 2949183 D PFAM: protein of unknown function DUF6 transmembrane; KEGG: sbc:SbBS512_E2535 threonine/homoserine efflux transporter RhtA; threonine and homoserine efflux system 2948296..2949183 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158332 YP_003037016.1 CDS ECBD_2811 NC_012947.1 2949482 2949985 D binds DNA in a non-sequence-specific manner and is abundant during stationary phase; forms a DNA-protein crystal that protects DNA from damage; required for normal starvation response and long-term stationary viability; forms a homododecameric complex and sequesters iron which provides protection against oxidative damage; DNA starvation/stationary phase protection protein Dps 2949482..2949985 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158333 YP_003037017.1 CDS glnH NC_012947.1 2950389 2951135 D similar to periplasmic-binding component of ABC transporters; glutamine ABC transporter periplasmic protein 2950389..2951135 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158334 YP_003037018.1 CDS glnP NC_012947.1 2951274 2951933 D similar to permease of ABC transporters; mutations impair ability of Escherichia coli to transport and utilize glutamine; glutamine ABC transporter permease 2951274..2951933 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158335 YP_003037019.1 CDS glnQ NC_012947.1 2951930 2952652 D similar to ATP-binding component of ABC transporters; ABC transporter ATP-binding protein 2951930..2952652 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158336 YP_003037020.1 CDS ECBD_2815 NC_012947.1 2952913 2955138 D PFAM: MscS Mechanosensitive ion channel; KEGG: efe:EFER_2300 hypothetical protein; hypothetical protein 2952913..2955138 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158337 YP_003037021.1 CDS ECBD_2816 NC_012947.1 2955135 2956061 R PFAM: protein of unknown function DUF890; KEGG: sbc:SbBS512_E2543 hypothetical protein; SAM-dependent methyltransferase complement(2955135..2956061) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158338 YP_003037022.1 CDS ECBD_2817 NC_012947.1 2956337 2956597 D PFAM: protein of unknown function DUF1471; KEGG: sbc:SbBS512_E2544 hypothetical protein; hypothetical protein 2956337..2956597 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158339 YP_003037023.1 CDS ECBD_2818 NC_012947.1 2956862 2959144 D Fiu; YbiL; porin involved in the uptake of iron complexed with catecholate siderophores (e.g. dihydroxybenzoylserine, dihydroxybenzoate) and beta lactam agents; Fiu interacts with TonB which provides the energy required for transport across the outer membrane; acts as a receptor for iron-siderophores and microcins E492, M, and H47; outer membrane protein; catecholate siderophore receptor Fiu 2956862..2959144 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158340 YP_003037024.1 CDS ECBD_2819 NC_012947.1 2959186 2959863 D PFAM: 2OG-Fe(II) oxygenase; SMART: prolyl 4-hydroxylase subunit alpha; hydroxylase 2959186..2959863 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158341 YP_003037025.1 CDS ECBD_2820 NC_012947.1 2959937 2960203 D PFAM: zinc finger DksA/TraR C4-type; KEGG: ssn:SSON_0782 hypothetical protein; hypothetical protein 2959937..2960203 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158342 YP_003037026.1 CDS ECBD_2821 NC_012947.1 2960468 2960728 D PFAM: protein of unknown function DUF1471; KEGG: sfv:SFV_0785 hypothetical protein; hypothetical protein 2960468..2960728 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158343 YP_003037027.1 CDS ECBD_2822 NC_012947.1 2960957 2962042 R PFAM: malate/L-lactate dehydrogenase; KEGG: ssn:SSON_0780 dehydrogenase; hypothetical protein complement(2960957..2962042) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158344 YP_003037028.1 CDS ECBD_2823 NC_012947.1 2962183 2963145 R PFAM: glycosyl transferase, family 3-like; KEGG: sbo:SBO_0688 hypothetical protein; glycosyl transferase complement(2962183..2963145) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158345 YP_003037029.1 CDS dinG NC_012947.1 2963173 2965458 R helicase involved in DNA repair and perhaps also replication; ATP-dependent DNA helicase DinG complement(2963173..2965458) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158346 YP_003037030.1 CDS ECBD_2825 NC_012947.1 2965476 2965925 D PFAM: conserved hypothetical protein; KEGG: efe:EFER_2310 hypothetical protein; hypothetical protein 2965476..2965925 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158347 YP_003037031.1 CDS ECBD_2826 NC_012947.1 2966157 2967521 R this helicase is not essential cell growth; ATP-dependent RNA helicase RhlE complement(2966157..2967521) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158348 YP_003037032.1 CDS ECBD_2827 NC_012947.1 2967750 2968421 D PFAM: TetR family transcriptional regulator ; regulatory protein TetR; KEGG: sfv:SFV_0779 DNA-binding transcriptional regulator; DNA-binding transcriptional regulator 2967750..2968421 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158349 YP_003037033.1 CDS ECBD_2828 NC_012947.1 2968424 2969419 D PFAM: secretion protein HlyD; KEGG: sfv:SFV_0778 hypothetical protein; hypothetical protein 2968424..2969419 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158350 YP_003037034.1 CDS ECBD_2829 NC_012947.1 2969412 2971148 D PFAM: ABC transporter; SMART: ATPase AAA; KEGG: hypothetical protein; ABC transporter 2969412..2971148 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158351 YP_003037035.1 CDS ECBD_2830 NC_012947.1 2971141 2972274 D PFAM: ABC transporter; KEGG: ssn:SSON_0772 hypothetical protein; ABC transporter 2971141..2972274 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158352 YP_003037036.1 CDS ECBD_2831 NC_012947.1 2972285 2973391 D PFAM: ABC transporter; KEGG: ssn:SSON_0771 hypothetical protein; ABC transporter 2972285..2973391 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158353 YP_003037037.1 CDS ECBD_2832 NC_012947.1 2973353 2973763 R KEGG: sbc:SbBS512_E2560 hypothetical protein; hypothetical protein complement(2973353..2973763) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158354 YP_003037038.1 CDS ECBD_2833 NC_012947.1 2973896 2974657 D PFAM: endonuclease/exonuclease/phosphatase; KEGG: ssn:SSON_0769 hypothetical protein; endonuclease/exonuclease/phosphatase 2973896..2974657 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158355 YP_003037039.1 CDS ECBD_2834 NC_012947.1 2974654 2975895 D PFAM: phospholipase D/transphosphatidylase; SMART: phospholipase D/transphosphatidylase; KEGG: elf:LF82_2631 cardiolipin synthetase YbhO; cardiolipin synthase 2 2974654..2975895 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158356 YP_003037040.1 CDS ECBD_2835 NC_012947.1 2975895 2976851 D hypothetical protein 2975895..2976851 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158357 YP_003037041.1 CDS ECBD_2836 NC_012947.1 2976887 2977600 R PFAM: protein of unknown function UPF0005; KEGG: sfv:SFV_0770 hypothetical protein; hypothetical protein complement(2976887..2977600) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158358 YP_003037042.1 CDS ECBD_2837 NC_012947.1 2977805 2978509 R PFAM: protein of unknown function UPF0005; KEGG: ssn:SSON_0765 hypothetical protein; hypothetical protein complement(2977805..2978509) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158359 YP_003037043.1 CDS moaE NC_012947.1 2978645 2979097 R catalyzes the conversion of molybdopterin precursor Z into molybdopterin; molybdopterin guanine dinucleotide biosynthesis protein MoaE complement(2978645..2979097) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158360 YP_003037044.1 CDS moaD NC_012947.1 2979099 2979344 R catalyzes the conversion of molybdopterin precursor Z into molybdopterin; molybdopterin synthase small subunit complement(2979099..2979344) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158361 YP_003037045.1 CDS moaC NC_012947.1 2979337 2979822 R MoaC; along with MoaA is involved in conversion of a guanosine derivative into molybdopterin precursor Z; involved in molybdenum cofactor biosynthesis; molybdenum cofactor biosynthesis protein MoaC complement(2979337..2979822) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158362 YP_003037046.1 CDS ECBD_2841 NC_012947.1 2979825 2980337 R TIGRFAM: molybdenum cofactor biosynthesis protein B; molybdenum cofactor synthesis protein; PFAM: molybdopterin binding domain; KEGG: ssn:SSON_0761 molybdopterin biosynthesis, protein B; molybdenum cofactor biosynthesis protein B complement(2979825..2980337) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158363 YP_003037047.1 CDS moaA NC_012947.1 2980359 2981348 R together with moaC, is involved in the conversion of a guanosine derivative (GXP) into molybdopterin precursor Z; molybdenum cofactor biosynthesis protein A complement(2980359..2981348) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158364 YP_003037048.1 CDS ECBD_2843 NC_012947.1 2981745 2982653 D PFAM: protein of unknown function UPF0052 and CofD; KEGG: ssn:SSON_0759 structural protein; hypothetical protein 2981745..2982653 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158365 YP_003037049.1 CDS ECBD_2844 NC_012947.1 2982845 2984866 R The UvrABC repair system catalyzes the recognition and processing of DNA lesions. The beta-hairpin of the Uvr-B subunit is inserted between the strands, where it probes for the presence of a lesion; excinuclease ABC subunit B complement(2982845..2984866) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158366 YP_003037050.1 CDS bioD NC_012947.1 2985445 2986122 R DTB synthetase; dethiobiotin synthase; involved in production of dethiobiotin from ATP and 7,8-diaminononanoate and carbon dioxide; contains magnesium; dithiobiotin synthetase complement(2985445..2986122) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158367 YP_003037051.1 CDS ECBD_2846 NC_012947.1 2986115 2986870 R methyltransferase; acyl carrier protein involved in an unidentified step in the synthesis of pimeloyl-CoA, a biotin precursor; member of the bio operon (bioABFCD); in Escherichia coli, bioC-null mutants require biotin for growth; biotin biosynthesis protein BioC complement(2986115..2986870) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158368 YP_003037052.1 CDS ECBD_2847 NC_012947.1 2986857 2988011 R catalyzes the formation of 8-amino-7-oxononanoate from 6-carboxyhexanoyl-CoA and L-alanine; 8-amino-7-oxononanoate synthase complement(2986857..2988011) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158369 YP_003037053.1 CDS ECBD_2848 NC_012947.1 2988008 2989048 R KEGG: ssn:SSON_0754 biotin synthesis; TIGRFAM: biotin synthase; PFAM: biotin and thiamin synthesis associated; radical SAM domain protein; SMART: Elongator protein 3/MiaB/NifB; biotin synthase complement(2988008..2989048) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158370 YP_003037054.1 CDS ECBD_2849 NC_012947.1 2989135 2990424 D catalyzes the formation of S-adenosyl-4-methylthionine-2-oxobutanoate and 7,8-diaminononanoate from S-adenosyl-L-methionine and 8-amino-7-oxononanoate; adenosylmethionine--8-amino-7-oxononanoate transaminase 2989135..2990424 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158371 YP_003037055.1 CDS ECBD_2850 NC_012947.1 2990483 2990959 D YbhB; similar to rat and human kinase inhibitory proteins; kinase inhibitor protein 2990483..2990959 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158372 YP_003037056.1 CDS ECBD_2851 NC_012947.1 2991255 2991416 R hypothetical protein complement(2991255..2991416) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158373 YP_003037057.1 CDS ECBD_2854 NC_012947.1 2994325 2994945 R PFAM: virulence-related outer membrane protein; KEGG: sbo:SBO_1623 membrane protein precursor; virulence-related outer membrane protein complement(2994325..2994945) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158376 YP_003037058.1 CDS ECBD_2855 NC_012947.1 2995007 2998405 R PFAM: protein of unknown function DUF1983; Fibronectin type III domain protein; KEGG: elf:LF82_p179 host specificity protein; hypothetical protein complement(2995007..2998405) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158377 YP_003037059.1 CDS ECBD_2856 NC_012947.1 2998466 2999038 R PFAM: lambda tail assembly I; KEGG: hypothetical protein; lambda tail assembly I complement(2998466..2999038) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158378 YP_003037060.1 CDS ECBD_2857 NC_012947.1 2999035 2999778 R PFAM: NLP/P60 protein; SMART: Mov34/MPN/PAD-1 family protein; NLP/P60 protein complement(2999035..2999778) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158379 YP_003037061.1 CDS ECBD_2858 NC_012947.1 2999784 3000482 R TIGRFAM: phage minor tail protein L; PFAM: minor tail protein L; KEGG: elf:LF82_p176 minor tail protein; phage minor tail protein L complement(2999784..3000482) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158380 YP_003037062.1 CDS ECBD_2859 NC_012947.1 3000482 3000811 R PFAM: minor tail family protein; KEGG: elf:LF82_p175 minor tail protein; minor tail family protein complement(3000482..3000811) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158381 YP_003037063.1 CDS ECBD_2860 NC_012947.1 3000808 3003369 R TIGRFAM: phage tail tape measure protein, lambda family; PFAM: Prophage tail length tape measure; KEGG: elf:LF82_p174 tail component of prophage; phage tail tape measure protein, lambda family complement(3000808..3003369) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158382 YP_003037064.1 CDS ECBD_2861 NC_012947.1 3003362 3003796 R TIGRFAM: phage tail assembly protein T; PFAM: Minor tail T family protein; KEGG: ssn:SSON_2418 tail component of prophage; phage tail assembly protein T complement(3003362..3003796) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158383 YP_003037065.1 CDS ECBD_2862 NC_012947.1 3003778 3004200 R TIGRFAM: phage minor tail protein G; PFAM: minor tail protein G; KEGG: ssn:SSON_2419 tail component of prophage; phage minor tail protein G complement(3003778..3004200) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158384 YP_003037066.1 CDS ECBD_2863 NC_012947.1 3004216 3004956 R PFAM: hypothetical protein; SMART: Ig domain protein group 2 domain protein; KEGG: elf:LF82_p171 tail component of prophage; hypothetical protein complement(3004216..3004956) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158385 YP_003037067.1 CDS ECBD_2864 NC_012947.1 3004964 3005359 R PFAM: minor tail U; minor tail U complement(3004964..3005359) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158386 YP_003037068.1 CDS ECBD_2865 NC_012947.1 3005356 3005934 R PFAM: Prophage minor tail Z family protein; KEGG: ssn:SSON_2422 tail component of prophage; Prophage minor tail Z family protein complement(3005356..3005934) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158387 YP_003037069.1 CDS ECBD_2866 NC_012947.1 3005946 3006299 R PFAM: head-tail attachment protein; KEGG: ssn:SSON_2423 head-tail joining protein of prophage; head-tail attachment protein complement(3005946..3006299) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158388 YP_003037070.1 CDS ECBD_2867 NC_012947.1 3006311 3006709 R KEGG: ssn:SSON_2424 DNA-packaging protein; DNA-packaging protein complement(3006311..3006709) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158389 YP_003037071.1 CDS ECBD_2868 NC_012947.1 3006751 3007776 R PFAM: major capsid protein E; KEGG: ssn:SSON_2425 major capsid protein; major capsid protein E complement(3006751..3007776) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158390 YP_003037072.1 CDS ECBD_2869 NC_012947.1 3007832 3008164 R PFAM: lambda head decoration protein D HDPD; KEGG: ssn:SSON_2426 head decoration protein; lambda head decoration protein D HDPD complement(3007832..3008164) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158391 YP_003037073.1 CDS ECBD_2870 NC_012947.1 3008174 3009493 R PFAM: peptidase S49; peptidase S49 complement(3008174..3009493) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158392 YP_003037074.1 CDS ECBD_2871 NC_012947.1 3009474 3011075 R TIGRFAM: phage portal protein, lambda family; PFAM: portal protein lambda; KEGG: ssn:SSON_2428 capsid structural protein of prophage; phage portal protein, lambda family complement(3009474..3011075) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158393 YP_003037075.1 CDS ECBD_2872 NC_012947.1 3011072 3011278 R PFAM: Head-to-tail joining protein W gpW family protein; KEGG: ssn:SSON_2429 head-tail joining protein of prophage; Head-to-tail joining protein W gpW family protein complement(3011072..3011278) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158394 YP_003037076.1 CDS ECBD_2873 NC_012947.1 3011275 3013200 R PFAM: terminase GpA; KEGG: ssn:SSON_2430 DNA packaging protein of prophage; terminase GpA complement(3011275..3013200) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158395 YP_003037077.1 CDS ECBD_2874 NC_012947.1 3013175 3013720 R PFAM: DNA packaging Nu1; KEGG: elf:LF82_1506 prophage QSR' DNA packaging protein NU1 homolog; DNA packaging Nu1 complement(3013175..3013720) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158396 YP_003037078.1 CDS ECBD_2875 NC_012947.1 3014109 3014303 D hypothetical protein 3014109..3014303 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158397 YP_003037079.1 CDS ECBD_2876 NC_012947.1 3014468 3014674 R KEGG: elf:LF82_2580 uncharacterized protein YbcW; hypothetical protein complement(3014468..3014674) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158398 YP_003037080.1 CDS ECBD_2877 NC_012947.1 3014960 3015370 D PFAM: protein of unknown function DUF1398; KEGG: elf:LF82_2579 uncharacterized protein YbcV; hypothetical protein 3014960..3015370 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158399 YP_003037081.1 CDS ECBD_2878 NC_012947.1 3015660 3015953 D PFAM: Bor family protein; KEGG: elf:LF82_0244 lipoprotein bor homolog from lambdoid prophage DLP12; Bor family protein 3015660..3015953 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158400 YP_003037082.1 CDS ECBD_2879 NC_012947.1 3015985 3016446 R PFAM: lysis protein; lysis protein complement(3015985..3016446) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158401 YP_003037083.1 CDS ECBD_2880 NC_012947.1 3016443 3016919 R PFAM: glycoside hydrolase family 24; KEGG: sec:SC0349 lysin; Lysozyme complement(3016443..3016919) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158402 YP_003037084.1 CDS ECBD_2881 NC_012947.1 3016903 3017226 R TIGRFAM: phage holin, lambda family; PFAM: Holin phage lambda; KEGG: sfv:SFV_0278 cell lysis protein; phage holin, lambda family complement(3016903..3017226) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158403 YP_003037085.1 CDS ECBD_2882 NC_012947.1 3017597 3017791 R hypothetical protein complement(3017597..3017791) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158404 YP_003037086.1 CDS ECBD_2883 NC_012947.1 3017903 3018526 R PFAM: antitermination protein; KEGG: sfv:SFV_0277 antitermination protein; antitermination protein complement(3017903..3018526) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158156 YP_003037087.1 CDS ECBD_2884 NC_012947.1 3018523 3019326 R PFAM: metallophosphoesterase; KEGG: efe:EFER_2055 serine/threonine-protein phosphatase; phosphoprotein phosphatase complement(3018523..3019326) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156861 YP_003037088.1 CDS ECBD_2885 NC_012947.1 3019323 3020024 R PFAM: replication P family protein; KEGG: elf:LF82_p157 replication protein P of bacteriophage; replication P family protein complement(3019323..3020024) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156862 YP_003037089.1 CDS ECBD_2886 NC_012947.1 3020021 3020920 R TIGRFAM: phage replication protein O; PFAM: replication protein O domain protein; phage replication protein O complement(3020021..3020920) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156863 YP_003037090.1 CDS ECBD_2887 NC_012947.1 3020953 3021252 R PFAM: CII family protein; KEGG: efe:EFER_2070 regulatory protein CII from phage origin; CII family protein complement(3020953..3021252) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156864 YP_003037091.1 CDS ECBD_2888 NC_012947.1 3021368 3021553 R KEGG: elf:LF82_p108 Cro protein; Cro protein complement(3021368..3021553) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156865 YP_003037092.1 CDS ECBD_2889 NC_012947.1 3021634 3022284 D PFAM: peptidase S24 and S26 domain protein; helix-turn-helix domain protein; SMART: helix-turn-helix domain protein; KEGG: elf:LF82_p107 repressor protein; XRE family transcriptional regulator 3021634..3022284 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156866 YP_003037093.1 CDS ECBD_2890 NC_012947.1 3022599 3022898 D KEGG: elf:LF82_p106 phage regulatory protein N; phage regulatory protein N 3022599..3022898 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156867 YP_003037094.1 CDS ECBD_2891 NC_012947.1 3022977 3023177 D KEGG: efe:EFER_2077 restriction inhibitor protein ral (antirestriction protein); restriction inhibitor protein ral (antirestriction protein) 3022977..3023177 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156868 YP_003037095.1 CDS ECBD_2892 NC_012947.1 3023360 3023728 D hypothetical protein 3023360..3023728 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156869 YP_003037096.1 CDS ECBD_2893 NC_012947.1 3023801 3023965 D PFAM: Lambda phage regulatory protein CIII; KEGG: sek:SSPA2258 hypothetical protein; Lambda phage regulatory protein CIII 3023801..3023965 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156870 YP_003037097.1 CDS ECBD_2894 NC_012947.1 3023808 3024077 D PFAM: lambda Kil; KEGG: ses:SARI_02674 hypothetical protein; lambda Kil 3023808..3024077 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156871 YP_003037098.1 CDS ECBD_2895 NC_012947.1 3024032 3024448 D PFAM: Host-nuclease inhibitor Gam; KEGG: efe:EFER_2726 host-nuclease inhibitor protein Gam of bacteriophage; Host-nuclease inhibitor Gam 3024032..3024448 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156872 YP_003037099.1 CDS ECBD_2896 NC_012947.1 3024454 3025239 D TIGRFAM: phage recombination protein Bet; PFAM: RecT protein; KEGG: efe:EFER_2727 recombination protein bet from phage origin; phage recombination protein Bet 3024454..3025239 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156873 YP_003037100.1 CDS ECBD_2897 NC_012947.1 3025236 3025916 D KEGG: efe:EFER_2728 exonuclease from phage origin; exodeoxyribonuclease (lambda-induced) 3025236..3025916 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156874 YP_003037101.1 CDS ECBD_2898 NC_012947.1 3025913 3026095 D PFAM: protein of unknown function DUF1317; KEGG: elf:LF82_p147 hypothetical protein; hypothetical protein 3025913..3026095 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156875 YP_003037102.1 CDS ECBD_2899 NC_012947.1 3026068 3026259 D PFAM: protein of unknown function DUF1382; KEGG: elf:LF82_p146 hypothetical protein; hypothetical protein 3026068..3026259 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156876 YP_003037103.1 CDS ECBD_2901 NC_012947.1 3026869 3027417 D PFAM: protein of unknown function DUF550; hypothetical protein 3026869..3027417 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156878 YP_003037104.1 CDS ECBD_2902 NC_012947.1 3027609 3027890 D hypothetical protein 3027609..3027890 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156879 YP_003037105.1 CDS ECBD_2903 NC_012947.1 3027979 3028146 D KEGG: elf:LF82_p097 hypothetical protein; hypothetical protein 3027979..3028146 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156880 YP_003037106.1 CDS ECBD_2904 NC_012947.1 3028186 3028404 D PFAM: excisionase; KEGG: elf:LF82_p095 excisionase; excisionase 3028186..3028404 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156881 YP_003037107.1 CDS ECBD_2905 NC_012947.1 3028382 3029305 D PFAM: lambda integrase ; integrase domain protein SAM domain protein; integrase; KEGG: sfv:SFV_2686 integrase; lambda integrase 3028382..3029305 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156882 YP_003037108.1 CDS ECBD_2906 NC_012947.1 3029313 3031964 R PFAM: DNA-directed RNA polymerase bacteriophage type; KEGG: ppu:PP_2266 DNA-directed RNA polymerase, bacteriophage and mitochondrial type; DNA-directed RNA polymerase complement(3029313..3031964) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156883 YP_003037109.1 CDS ECBD_2907 NC_012947.1 3031995 3032444 R PFAM: glycoside hydrolase family 2 sugar binding; KEGG: elf:LF82_1168 beta-galactosidase; beta-galactosidase complement(3031995..3032444) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156884 YP_003037110.1 CDS lacI NC_012947.1 3032567 3033658 R transcriptional repressor of lac operon; forms a homotetramer as a dimer of dimers; binds specific sites in lac operon resulting in DNA looping between the operators; binds allolactose as inducer; lac repressor complement(3032567..3033658) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156885 YP_003037111.1 CDS ECBD_2910 NC_012947.1 3034036 3035319 D outer membrane lipoprotein that catalyzes the hydrolysis of the thioester bond in palmitoyl-CoA; YbhC localizes to the cellular poles; member of pectinesterase family; pectinesterase 3034036..3035319 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156887 YP_003037112.1 CDS gpmA NC_012947.1 3036382 3037134 D 2,3-bisphosphoglycerate-dependent; catalyzes the interconversion of 2-phosphoglycerate to 3-phosphoglycerate; phosphoglyceromutase 3036382..3037134 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156889 YP_003037113.1 CDS ECBD_2913 NC_012947.1 3037292 3038344 R catalyzes the formation of 3-deoxy-D-arabino-hept-2-ulosonate 7 phosphate from phosphoenolpyruvate and D-erythrose 4-phosphate, phenylalanine sensitive; phospho-2-dehydro-3-deoxyheptonate aldolase complement(3037292..3038344) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156890 YP_003037114.1 CDS ECBD_2914 NC_012947.1 3038660 3039040 D KEGG: sfl:SF0551 homeobox protein; homeobox protein 3038660..3039040 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156891 YP_003037115.1 CDS ECBD_2915 NC_012947.1 3039154 3040095 D involved in zinc efflux across the cytoplasmic membrane; zinc transporter ZitB 3039154..3040095 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156892 YP_003037116.1 CDS ECBD_2916 NC_012947.1 3040092 3040811 R TIGRFAM: nicotinamide mononucleotide transporter PnuC; PFAM: Nicotinamide mononucleotide transporter PnuC; KEGG: ssn:SSON_0703 required for NMN transport; nicotinamide mononucleotide transporter PnuC complement(3040092..3040811) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156893 YP_003037117.1 CDS ECBD_2917 NC_012947.1 3040849 3041892 R 3 different subfamilies; catalyzes the formation of quinolinate from iminoaspartate and dihydroxyacetone phosphate; quinolinate synthetase complement(3040849..3041892) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156894 YP_003037118.1 CDS ECBD_2918 NC_012947.1 3043335 3044126 R periplasmic protein that interacts with TolA; the tol-pal system is probably involved in maintaining cell envelope integrity; tol-pal system protein YbgF complement(3043335..3044126) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156902 YP_003037119.1 CDS ECBD_2919 NC_012947.1 3044136 3044657 R TIGRFAM: peptidoglycan-associated lipoprotein; PFAM: OmpA/MotB domain protein; KEGG: sfx:S0569 peptidoglycan-associated outer membrane lipoprotein; peptidoglycan-associated outer membrane lipoprotein complement(3044136..3044657) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156903 YP_003037120.1 CDS tolB NC_012947.1 3044692 3045984 R forms dimers; may be involved in cell envelope integrity; interacts with outer membrane proteins and with the C-terminal domain of inner membrane protein TolA; translocation protein TolB complement(3044692..3045984) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156904 YP_003037121.1 CDS tolA NC_012947.1 3046117 3047382 R inner membrane component of 7 member Tol-Pal envelope-spanning complex; involved in maintaining cell envelope integrity; utilized by colicins and filamentous phages for import; interacts with TolB, Pal, and through TolB to various outer membrane porins; cell envelope integrity inner membrane protein TolA complement(3046117..3047382) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156905 YP_003037122.1 CDS ECBD_2922 NC_012947.1 3047447 3047875 R membrane spanning protein in TolA-TolQ-TolR complex; involved in the tonB-independent uptake of group A colicins; colicin uptake protein TolR complement(3047447..3047875) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156906 YP_003037123.1 CDS ECBD_2923 NC_012947.1 3047879 3048571 R membrane spanning protein in TolA-TolQ-TolR complex; involved in the tonB-independent uptake of group A colicins; colicin uptake protein TolQ complement(3047879..3048571) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156907 YP_003037124.1 CDS ECBD_2924 NC_012947.1 3048568 3048972 R catalyzes the hydrolysis of short chain aliphatic acyl-CoA thioesters; physiological role remains unknown; involved in phospholipid metabolism; part of the Tol/Pal system of proteins that are critical for maintaining the integrity of the cell envelope components; acyl-CoA thioester hydrolase YbgC complement(3048568..3048972) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156908 YP_003037125.1 CDS ECBD_2925 NC_012947.1 3049122 3049415 R TIGRFAM: cyd operon protein YbgE; KEGG: sdy:SDY_0683 hypothetical protein; hypothetical protein complement(3049122..3049415) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156909 YP_003037126.1 CDS ECBD_2926 NC_012947.1 3049415 3049528 R TIGRFAM: cyd operon protein YbgT; PFAM: membrane bound YbgT family protein; KEGG: sdy:SDY_0682 outer membrane lipoprotein; cyd operon protein YbgT complement(3049415..3049528) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156910 YP_003037127.1 CDS ECBD_2927 NC_012947.1 3049543 3050682 R TIGRFAM: cytochrome d ubiquinol oxidase subunit II; PFAM: cytochrome bd ubiquinol oxidase subunit II; KEGG: sbc:SbBS512_E0655 cytochrome d ubiquinol oxidase subunit II; cytochrome d ubiquinol oxidase subunit II complement(3049543..3050682) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156911 YP_003037128.1 CDS ECBD_2928 NC_012947.1 3050698 3052266 R PFAM: cytochrome bd ubiquinol oxidase subunit I; KEGG: ssn:SSON_0685 cytochrome d terminal oxidase polypeptide subunit I; cytochrome bd ubiquinol oxidase subunit I complement(3050698..3052266) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156912 YP_003037129.1 CDS ECBD_2929 NC_012947.1 3053113 3055746 R PFAM: glycoside hydrolase family 38; glycosyl hydrolase 38 domain protein; KEGG: sfv:SFV_0604 alpha-mannosidase; alpha-mannosidase complement(3053113..3055746) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156913 YP_003037130.1 CDS ECBD_2930 NC_012947.1 3055764 3057680 R involved in the phosphorylation and transport of sugars across the cell membrane; protein IIA transfers a phosphoryl group to IIB which then transfers the phosphoryl group to the sugar; IIC forms the translocation channel for the sugar uptake; PTS system 2-O-a-mannosyl-D-glycerate specific transporter subunit IIABC complement(3055764..3057680) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156914 YP_003037131.1 CDS ECBD_2931 NC_012947.1 3057849 3058571 D regulates the succiny-lCoA synthetase operon; DNA-binding transcriptional repressor MngR 3057849..3058571 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156915 YP_003037132.1 CDS ECBD_2932 NC_012947.1 3058675 3059544 R Catalyzes the only substrate-level phosphorylation in the TCA cycle; succinyl-CoA synthetase subunit alpha complement(3058675..3059544) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156916 YP_003037133.1 CDS sucC NC_012947.1 3059544 3060710 R catalyzes the interconversion of succinyl-CoA and succinate; succinyl-CoA synthetase subunit beta complement(3059544..3060710) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156917 YP_003037134.1 CDS ECBD_2934 NC_012947.1 3061076 3062293 R component of 2-oxoglutarate dehydrogenase complex; catalyzes the transfer of succinyl coenzyme A to form succinyl CoA as part of the conversion of 2-oxoglutarate to succinyl-CoA; dihydrolipoamide succinyltransferase complement(3061076..3062293) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156918 YP_003037135.1 CDS sucA NC_012947.1 3062308 3065109 R SucA; E1 component of the oxoglutarate dehydrogenase complex which catalyzes the formation of succinyl-CoA from 2-oxoglutarate; SucA catalyzes the reaction of 2-oxoglutarate with dihydrolipoamide succinyltransferase-lipoate to form dihydrolipoamide succinyltransferase-succinyldihydrolipoate and carbon dioxide; 2-oxoglutarate dehydrogenase E1 component complement(3062308..3065109) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156919 YP_003037136.1 CDS sdhB NC_012947.1 3065410 3066126 R part of four member succinate dehydrogenase enzyme complex that forms a trimeric complex (trimer of tetramers); SdhA/B are the catalytic subcomplex and can exhibit succinate dehydrogenase activity in the absence of SdhC/D which are the membrane components and form cytochrome b556; SdhC binds ubiquinone; oxidizes succinate to fumarate while reducing ubiquinone to ubiquinol; the catalytic subunits are similar to fumarate reductase; succinate dehydrogenase iron-sulfur subunit complement(3065410..3066126) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156920 YP_003037137.1 CDS sdhA NC_012947.1 3066142 3067908 R part of four member succinate dehydrogenase enzyme complex that forms a trimeric complex (trimer of tetramers); SdhA/B are the catalytic subcomplex and can exhibit succinate dehydrogenase activity in the absence of SdhC/D which are the membrane components and form cytochrome b556; SdhC binds ubiquinone; oxidizes succinate to fumarate while reducing ubiquinone to ubiquinol; succinate dehydrogenase flavoprotein subunit complement(3066142..3067908) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156921 YP_003037138.1 CDS sdhD NC_012947.1 3067908 3068255 R TIGRFAM: succinate dehydrogenase, hydrophobic membrane anchor protein; KEGG: ssn:SSON_0673 succinate dehydrogenase cytochrome b556 small membrane subunit; succinate dehydrogenase cytochrome b556 small membrane subunit complement(3067908..3068255) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156922 YP_003037139.1 CDS sdhC NC_012947.1 3068249 3068653 R TIGRFAM: succinate dehydrogenase, cytochrome b556 subunit; PFAM: succinate dehydrogenase cytochrome b subunit; KEGG: sbc:SbBS512_E0640 succinate dehydrogenase, cytochrome b556 subunit; succinate dehydrogenase cytochrome b556 large membrane subunit complement(3068249..3068653) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156923 YP_003037140.1 CDS ECBD_2940 NC_012947.1 3069034 3069141 D KEGG: efe:EFER_2393 hypothetical protein; hypothetical protein 3069034..3069141 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156924 YP_003037141.1 CDS gltA NC_012947.1 3069347 3070630 D type II enzyme; in Escherichia coli this enzyme forms a trimer of dimers which is allosterically inhibited by NADH and competitively inhibited by alpha-ketoglutarate; allosteric inhibition is lost when Cys206 is chemically modified which also affects hexamer formation; forms oxaloacetate and acetyl-CoA and water from citrate and coenzyme A; functions in TCA cycle, glyoxylate cycle and respiration; enzyme from Helicobacter pylori is not inhibited by NADH; type II citrate synthase 3069347..3070630 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156925 YP_003037142.1 CDS ECBD_2942 NC_012947.1 3071020 3071586 D PFAM: fimbrial protein; KEGG: ssn:SSON_0670 fimbrial-like protein; fimbrial protein 3071020..3071586 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156926 YP_003037143.1 CDS ECBD_2943 NC_012947.1 3071637 3074096 D PFAM: fimbrial biogenesis outer membrane usher protein; KEGG: sfx:S0592 outer membrane protein; fimbrial biogenesis outer membrane usher protein 3071637..3074096 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156927 YP_003037144.1 CDS ECBD_2944 NC_012947.1 3074111 3074842 D PFAM: Pili assembly chaperone, N-terminal; Pili assembly chaperone, C-terminal; KEGG: sbc:SbBS512_E0634 periplasmic pilus chaperone family protein; Pili assembly chaperone, N-terminal 3074111..3074842 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156928 YP_003037145.1 CDS ECBD_2945 NC_012947.1 3074839 3075900 D KEGG: ssn:SSON_0667 hypothetical protein; hypothetical protein 3074839..3075900 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156929 YP_003037146.1 CDS ECBD_2946 NC_012947.1 3076052 3077098 D TIGRFAM: membrane protein AbrB duplication; PFAM: ammonia monooxygenase; KEGG: ssn:SSON_0666 transporter; membrane protein AbrB duplication 3076052..3077098 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156930 YP_003037147.1 CDS ECBD_2947 NC_012947.1 3077095 3077886 R 5-formyluracil/5-hydroxymethyluracil DNA glycosylase; involved in base excision repair of DNA damaged by oxidation or by mutagenic agents; acts as DNA glycosylase that recognizes and removes damaged bases with a preference for oxidized pyrimidines; has apurinic/apyrimidinic lyase activity; endonuclease VIII complement(3077095..3077886) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156931 YP_003037148.1 CDS ECBD_2948 NC_012947.1 3077922 3078656 R PFAM: LamB/YcsF family protein; KEGG: sbo:SBO_0572 hypothetical protein; LamB/YcsF family protein complement(3077922..3078656) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156932 YP_003037149.1 CDS ECBD_2949 NC_012947.1 3078646 3079578 R TIGRFAM: urea amidolyase; PFAM: allophanate hydrolase subunit 2; SMART: allophanate hydrolase subunit 2; urea amidolyase complement(3078646..3079578) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156933 YP_003037150.1 CDS ECBD_2950 NC_012947.1 3079572 3080228 R PFAM: allophanate hydrolase subunit 1; SMART: allophanate hydrolase subunit 1; allophanate hydrolase subunit 1 complement(3079572..3080228) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156934 YP_003037151.1 CDS ECBD_2951 NC_012947.1 3080251 3080994 R PFAM: protein of unknown function DUF34; KEGG: sbc:SbBS512_E0627 NIF3 family protein; hydrolase-oxidase complement(3080251..3080994) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156935 YP_003037152.1 CDS ECBD_2952 NC_012947.1 3081265 3082746 D TIGRFAM: amino acid/peptide transporter; PFAM: TGF-beta receptor type I/II extracellular region; major facilitator superfamily MFS_1; KEGG: sfx:S0601 transporter; amino acid/peptide transporter 3081265..3082746 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156936 YP_003037153.1 CDS ECBD_2953 NC_012947.1 3082785 3084206 R UV-induced DNA repair; converts cyclobutane-type pyrimidine dimers created during exposure to UV ratiation to monomers; light dependent; deoxyribodipyrimidine photolyase complement(3082785..3084206) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156937 YP_003037154.1 CDS ECBD_2954 NC_012947.1 3084203 3084712 R PFAM: protein of unknown function DUF1722; KEGG: sfv:SFV_0628 hypothetical protein; hypothetical protein complement(3084203..3084712) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158157 YP_003037155.1 CDS ECBD_2955 NC_012947.1 3084860 3085621 R PFAM: transposase IS4 family protein; KEGG: ssn:SSON_0260 receptor; transposase IS4 family protein complement(3084860..3085621) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158158 YP_003037156.1 CDS ECBD_2957 NC_012947.1 3088643 3089212 R hypothetical protein complement(3088643..3089212) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158160 YP_003037157.1 CDS ECBD_2959 NC_012947.1 3090760 3091086 R hypothetical protein complement(3090760..3091086) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159157 YP_003037158.1 CDS ECBD_2961 NC_012947.1 3093171 3093377 R KEGG: sdy:SDY_0635 hypothetical protein; hypothetical protein complement(3093171..3093377) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159159 YP_003037159.1 CDS ECBD_2962 NC_012947.1 3093779 3095452 D catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions; potassium-transporting ATPase A 3093779..3095452 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159160 YP_003037160.1 CDS ECBD_2963 NC_012947.1 3095475 3097523 D One of the components of the high-affinity ATP-driven potassium transport (or KDP) system, which catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions; potassium-transporting ATPase B 3095475..3097523 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159161 YP_003037161.1 CDS ECBD_2964 NC_012947.1 3097532 3098104 D One of the components of the high-affinity ATP-driven potassium transport (or KDP)system, which catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions. The C subunit may be involved in assembly of the KDP complex; potassium-transporting ATPase C 3097532..3098104 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159162 YP_003037162.1 CDS ECBD_2965 NC_012947.1 3098097 3100781 D sensory histidine kinase in two-component regulatory system with KdpE; signal sensing protein; sensor protein KdpD 3098097..3100781 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159163 YP_003037163.1 CDS ECBD_2966 NC_012947.1 3100778 3101455 D response regulator in two-component regulatory system with KdpD; regulates the kdp operon involved in potassium transport; DNA-binding transcriptional activator KdpE 3100778..3101455 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159164 YP_003037164.1 CDS ECBD_2967 NC_012947.1 3101671 3101928 R KEGG: sbo:SBO_0555 hypothetical protein; hypothetical protein complement(3101671..3101928) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159165 YP_003037165.1 CDS ECBD_2968 NC_012947.1 3102051 3104249 D PFAM: Orn/Lys/Arg decarboxylase major region; Orn/Lys/Arg decarboxylase domain protein; KEGG: sbo:SBO_0554 ornithine decarboxylase isozyme, inducible; ornithine decarboxylase 3102051..3104249 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159166 YP_003037166.1 CDS potE NC_012947.1 3104246 3105565 D catalyzes the uptake of putrescine via a proton symport mechanism, as well as the efflux of putrescine by a putrescine/ornithine antiport system; putrescine transporter 3104246..3105565 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159167 YP_003037167.1 CDS ECBD_2971 NC_012947.1 3105914 3106564 D KEGG: sbo:SBO_0552 hypothetical protein; hypothetical protein 3105914..3106564 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159169 YP_003037168.1 CDS ECBD_2972 NC_012947.1 3106605 3107099 R KEGG: sbo:SBO_0551 pectinase; pectinase complement(3106605..3107099) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159170 YP_003037169.1 CDS ECBD_2973 NC_012947.1 3107313 3108953 R catalyzes the interconversion of alpha-D-glucose 1-phosphate to alpha-D-glucose 6-phosphate; phosphoglucomutase complement(3107313..3108953) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159171 YP_003037170.1 CDS ECBD_2974 NC_012947.1 3108979 3109524 R negative modulator of the initiation of chromosome replication; replication initiation regulator SeqA complement(3108979..3109524) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159172 YP_003037171.1 CDS ECBD_2975 NC_012947.1 3109709 3110473 D PFAM: alpha/beta hydrolase fold; PGAP1 family protein; KEGG: sbo:SBO_0548 hypothetical protein; hypothetical protein 3109709..3110473 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159173 YP_003037172.1 CDS ECBD_2976 NC_012947.1 3110544 3110906 D PFAM: CopG domain protein DNA-binding domain protein; KEGG: ssn:SSON_0639 LexA regulated protein; LexA regulated protein 3110544..3110906 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159174 YP_003037173.1 CDS ECBD_2977 NC_012947.1 3111046 3111576 D An electron-transfer protein; flavodoxin binds one FMN molecule, which serves as a redox-active prosthetic group; flavodoxin FldA 3111046..3111576 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159175 YP_003037174.1 CDS fur NC_012947.1 3111865 3112311 D negatively regulates a number of operons that encode enzymes involved in iron transport; activated by manganese; forms a homodimer; ferric uptake regulator 3111865..3112311 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159176 YP_003037175.1 CDS ECBD_2979 NC_012947.1 3112395 3112721 R KEGG: sfl:SF0611 hypothetical protein; hypothetical protein complement(3112395..3112721) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159177 YP_003037176.1 CDS ECBD_2980 NC_012947.1 3112771 3114177 R PFAM: outer membrane porin; KEGG: sbc:SbBS512_E0569 outer membrane porin, OprD family; outer membrane porin complement(3112771..3114177) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159178 YP_003037177.1 CDS ECBD_2981 NC_012947.1 3114754 3116418 R catalyzes a two-step reaction, first charging a glutamine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; glutaminyl-tRNA synthetase complement(3114754..3116418) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159179 YP_003037178.1 CDS ECBD_2982 NC_012947.1 3116529 3116651 D hypothetical protein 3116529..3116651 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159180 YP_003037179.1 CDS ECBD_2983 NC_012947.1 3116621 3118567 R phosphoenolpyruvate-dependent sugar phosphotransferase system; catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane; IIB is phosphorylated by IIA and then transfers the phosphoryl group to the sugar; IIC forms the translocation channel; PTS system N-acetyl glucosamine specific transporter subunits IIABC complement(3116621..3118567) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159181 YP_003037180.1 CDS nagB NC_012947.1 3118900 3119700 D catalyzes the reversible formation of fructose 6-phosphate from glucosamine 6-phosphate; glucosamine-6-phosphate deaminase 3118900..3119700 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159182 YP_003037181.1 CDS nagA NC_012947.1 3119760 3120908 D catalyzes the formation of glucosamine 6-phosphate from N-acetylglucosamine 6-phosphate; N-acetylglucosamine-6-phosphate deacetylase 3119760..3120908 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159183 YP_003037182.1 CDS ECBD_2986 NC_012947.1 3120917 3122137 D PFAM: ROK family protein; KEGG: ssn:SSON_0630 transcriptional repressor of nag (N-acetylglucosamine) operon; ROK family protein 3120917..3122137 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159184 YP_003037183.1 CDS ECBD_2987 NC_012947.1 3122185 3122937 D TIGRFAM: HAD-superfamily hydrolase, subfamily IIA; PFAM: haloacid dehalogenase; KEGG: sdy:SDY_0609 UMP phosphatase; UMP phosphatase 3122185..3122937 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159185 YP_003037184.1 CDS asnB NC_012947.1 3123334 3124998 D functions in asparagine biosynthesis; converts glutamine, aspartate, ATP, and water to glutamate, asparagine, pyrophosphate and AMP; asparagine synthetase B 3123334..3124998 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159186 YP_003037185.1 CDS ubiF NC_012947.1 3126234 3127409 R catalyzes the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinol from 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol; functions in the biosynthesis of ubiquinone or coenzyme Q; 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase complement(3126234..3127409) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159194 YP_003037186.1 CDS ECBD_2990 NC_012947.1 3127555 3128979 D catalyzes the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine from N6-(dimethylallyl)adenosine (i(6)A); (dimethylallyl)adenosine tRNA methylthiotransferase 3127555..3128979 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159195 YP_003037187.1 CDS ECBD_2991 NC_012947.1 3129093 3130172 D PFAM: PhoH family protein; KEGG: sdy:SDY_0596 ATP-binding protein in pho regulon; PhoH family protein 3129093..3130172 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159196 YP_003037188.1 CDS ECBD_2992 NC_012947.1 3130169 3130636 D PFAM: protein of unknown function UPF0054; KEGG: sbo:SBO_0523 hypothetical protein; metalloprotease 3130169..3130636 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159197 YP_003037189.1 CDS ECBD_2993 NC_012947.1 3130726 3131604 D PFAM: transporter-associated region; CBS domain containing protein; SMART: CBS domain containing protein; KEGG: ssn:SSON_0612 transporter; transporter-associated region 3130726..3131604 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159198 YP_003037190.1 CDS lnt NC_012947.1 3131629 3133167 D transfers the fatty acyl group on membrane lipoproteins; apolipoprotein N-acyltransferase 3131629..3133167 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159199 YP_003037191.1 CDS ECBD_2995 NC_012947.1 3133294 3135171 R TIGRFAM: 40-residue YVTN family beta-propeller repeat protein; PFAM: rhomboid family protein; KEGG: ssn:SSON_0610 hypothetical protein; 40-residue YVTN family beta-propeller repeat protein complement(3133294..3135171) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159200 YP_003037192.1 CDS ECBD_2996 NC_012947.1 3135626 3136534 D PFAM: extracellular solute-binding protein family 3; SMART: extracellular solute-binding protein family 3; KEGG: sfv:SFV_0671 glutamate and aspartate transporter subunit; glutamate and aspartate transporter subunit 3135626..3136534 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159201 YP_003037193.1 CDS ECBD_2997 NC_012947.1 3136704 3137444 D TIGRFAM: polar amino acid ABC transporter inner membrane subunit; PFAM: binding-protein-dependent transporters inner membrane component; KEGG: efe:EFER_2449 glutamate and aspartate transporter subunit ; membrane component of ABC superfamily; polar amino acid ABC transporter permease 3136704..3137444 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159202 YP_003037194.1 CDS ECBD_2998 NC_012947.1 3137444 3138118 D TIGRFAM: polar amino acid ABC transporter inner membrane subunit; PFAM: binding-protein-dependent transporters inner membrane component; KEGG: sdy:SDY_0588 glutamate/aspartate transporter permease; polar amino acid ABC transporter permease 3137444..3138118 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159203 YP_003037195.1 CDS ECBD_3000 NC_012947.1 3138501 3139352 R PFAM: Integrase catalytic region; KEGG: sfv:SFV_3530 IS150 ORF2; Integrase catalytic region complement(3138501..3139352) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159205 YP_003037196.1 CDS ECBD_3001 NC_012947.1 3139349 3139870 R KEGG: sfx:S1483 IS103 orf; IS103 orf complement(3139349..3139870) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159206 YP_003037197.1 CDS rihA NC_012947.1 3140407 3141342 D Hydrolyzes with equal efficiency cytidine or uridine to ribose and cytosine or uracil, respectively; pyrimidine-specific; ribonucleoside hydrolase 1 3140407..3141342 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159207 YP_003037198.1 CDS ECBD_3003 NC_012947.1 3141426 3143096 D PFAM: heat shock protein 70; KEGG: ssn:SSON_0604 DnaK protein; heat shock protein 70 3141426..3143096 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159208 YP_003037199.1 CDS ECBD_3005 NC_012947.1 3144603 3145310 R PFAM: protein of unknown function DUF1266; KEGG: sbo:SBO_0509 hypothetical protein; hypothetical protein complement(3144603..3145310) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159210 YP_003037200.1 CDS ECBD_3006 NC_012947.1 3145474 3145986 D PFAM: Sel1 domain protein repeat-containing protein; SMART: Sel1 domain protein repeat-containing protein; KEGG: sdy:SDY_0568 hypothetical protein; Sel1 domain protein repeat-containing protein 3145474..3145986 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159211 YP_003037201.1 CDS ECBD_3007 NC_012947.1 3146155 3146451 D PFAM: Sel1 domain protein repeat-containing protein; SMART: Sel1 domain protein repeat-containing protein; KEGG: sbc:SbBS512_E0607 hypothetical protein; Sel1 domain protein repeat-containing protein 3146155..3146451 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159212 YP_003037202.1 CDS ECBD_3008 NC_012947.1 3146521 3147003 R PFAM: protein of unknown function DUF1451; KEGG: ssn:SSON_0597 hypothetical protein; hypothetical protein complement(3146521..3147003) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159213 YP_003037203.1 CDS leuS NC_012947.1 3147238 3149820 D leucine--tRNA ligase; LeuRS; class-I aminoacyl-tRNA synthetase; charges leucine by linking carboxyl group to alpha-phosphate of ATP and then transfers aminoacyl-adenylate to its tRNA; due to the large number of codons that tRNA(Leu) recognizes, the leucyl-tRNA synthetase does not recognize the anticodon loop of the tRNA, but instead recognition is dependent on a conserved discriminator base A37 and a long arm; an editing domain hydrolyzes misformed products; in Methanothermobacter thermautotrophicus this enzyme associates with prolyl-tRNA synthetase; leucyl-tRNA synthetase 3147238..3149820 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159214 YP_003037204.1 CDS ECBD_3010 NC_012947.1 3149835 3150416 D rare lipoprotein B; involved in the assembly of LPS in the outer membrane; LPS-assembly lipoprotein RlpB 3149835..3150416 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159215 YP_003037205.1 CDS holA NC_012947.1 3150416 3151447 D required for the assembly and function of the DNAX complex which is required for the assembly of the subunit beta onto primed DNA; DNA polymerase III subunit delta 3150416..3151447 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159216 YP_003037206.1 CDS nadD NC_012947.1 3151449 3152090 D transfers an adenyl group from ATP to NaMN to form nicotinic acid adenine dinucleotide (NaAD) which is then converted to the ubiquitous compound NAD by NAD synthetase; essential enzyme in bacteria; nicotinic acid mononucleotide adenylyltransferase 3151449..3152090 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159217 YP_003037207.1 CDS ECBD_3013 NC_012947.1 3152114 3152725 D PFAM: phosphoglycerate mutase; KEGG: sfv:SFV_0688 phosphohistidine protein PhpB; phosphoglycerate mutase 3152114..3152725 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159218 YP_003037208.1 CDS ECBD_3014 NC_012947.1 3152985 3153302 D PFAM: Iojap-related protein; KEGG: sbc:SbBS512_E0614 iojap family protein; hypothetical protein 3152985..3153302 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159219 YP_003037209.1 CDS ECBD_3015 NC_012947.1 3153306 3153773 D SPOUT methyltransferase family protein; crystal structure shows homodimer; in Escherichia coli this protein methylates pseudouridine at position 1915 of the 23S ribosomal RNA; rRNA large subunit methyltransferase 3153306..3153773 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159220 YP_003037210.1 CDS ECBD_3016 NC_012947.1 3153804 3155705 D PFAM: penicillin-binding protein transpeptidase; Penicillin-binding protein dimerisation domain; penicillin-binding protein 2 3153804..3155705 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159221 YP_003037211.1 CDS ECBD_3017 NC_012947.1 3155708 3156820 D PFAM: cell cycle protein; KEGG: sbc:SbBS512_E0617 rod shape-determining protein RodA; cell wall shape-determining protein 3155708..3156820 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159222 YP_003037212.1 CDS ECBD_3018 NC_012947.1 3156831 3157919 D PFAM: Rare lipoprotein A; sporulation domain protein; KEGG: ssn:SSON_0587 lipoprotein A; lipoprotein A 3156831..3157919 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159223 YP_003037213.1 CDS ECBD_3019 NC_012947.1 3158059 3159270 D penicillin-binding protein 5; removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors; D-alanyl-D-alanine carboxypeptidase fraction A 3158059..3159270 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159224 YP_003037214.1 CDS ECBD_3020 NC_012947.1 3159380 3159643 D PFAM: protein of unknown function DUF493; KEGG: ssn:SSON_0585 hypothetical protein; hypothetical protein 3159380..3159643 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159225 YP_003037215.1 CDS ECBD_3021 NC_012947.1 3159744 3160385 D lipoyl/octanoyltransferase; catalyzes the transfer of the lipoyl/octanoyl moiety of lipoyl/octanoyl-ACP onto lipoate-dependent enzymes like pyruvate dehydrogenase and the glycine cleavage system protein H; lipoate-protein ligase B 3159744..3160385 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159226 YP_003037216.1 CDS ECBD_3022 NC_012947.1 3160644 3161597 D PFAM: regulatory protein LysR; LysR substrate-binding; KEGG: sfv:SFV_0697 DNA-binding transcriptional regulator; DNA-binding transcriptional regulator 3160644..3161597 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159227 YP_003037217.1 CDS ECBD_3023 NC_012947.1 3161806 3162771 D catalyzes the radical-mediated insertion of two sulfur atoms into an acyl carrier protein (ACP) bound to an octanoyl group to produce a lipoyl group; lipoyl synthase 3161806..3162771 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159228 YP_003037218.1 CDS tatE NC_012947.1 3162872 3163075 R TatE; similar to TatA and found in some proteobacteria; part of system that translocates proteins with a conserved twin arginine motif across the inner membrane; capable of translocating folded substrates typically those with bound cofactors; similar to a protein import system in thylakoid membranes; twin arginine translocase protein E complement(3162872..3163075) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159229 YP_003037219.1 CDS ECBD_3025 NC_012947.1 3163204 3163992 R PFAM: nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase; KEGG: sbc:SbBS512_E0540 hydrolase, carbon-nitrogen family; nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase complement(3163204..3163992) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159230 YP_003037220.1 CDS ECBD_3026 NC_012947.1 3164085 3164468 D may be involved in chromosome condensation; overexpression in Escherichia coli protects against decondensation by camphor; overexpressing the protein results in an increase in supercoiling; camphor resistance protein CrcB 3164085..3164468 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159231 YP_003037221.1 CDS cspE NC_012947.1 3164523 3164732 R member of the CspA family; constitutively expressed RNA/ssDNA-binding protein; functions in helping cells adapt to low temperature; aids nucleic acid melting; participates in gene regulation; can act as a transcription antiterminator; cold shock protein CspE complement(3164523..3164732) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159232 YP_003037222.1 CDS pagP NC_012947.1 3164901 3165461 R catalyzes the transfer of palmitate to lipid A; palmitoyl transferase complement(3164901..3165461) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159233 YP_003037223.1 CDS dcuC NC_012947.1 3166049 3167434 D responsible for the transport of C4-dicarboxylates during anaerobic growth; C4-dicarboxylate transporter DcuC 3166049..3167434 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159234 YP_003037224.1 CDS dpiA NC_012947.1 3167475 3168155 R regulates the expression of citAB in citrate fermentation; two-component response regulator DpiA complement(3167475..3168155) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159235 YP_003037225.1 CDS ECBD_3031 NC_012947.1 3168124 3169782 R PFAM: ATP-binding region ATPase domain protein; SMART: ATP-binding region ATPase domain protein; PAS domain containing protein; signal transduction histidine kinase regulating citrate/malate metabolism complement(3168124..3169782) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159237 YP_003037226.1 CDS ECBD_3032 NC_012947.1 3169915 3170190 D PFAM: Insertion element protein; KEGG: dps:DP0861 insertion element iso-IS1d protein (InsA); Insertion element protein 3169915..3170190 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159238 YP_003037227.1 CDS ECBD_3033 NC_012947.1 3170109 3170612 D PFAM: IS1 transposase; KEGG: sty:HCM1.136a IS1 transposase; IS1 transposase 3170109..3170612 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159239 YP_003037228.1 CDS ECBD_3034 NC_012947.1 3170698 3171756 D TIGRFAM: citrate lyase ligase; cytidyltransferase-related domain protein; PFAM: citrate lyase ligase domain protein; GCN5-related N-acetyltransferase; SMART: citrate lyase ligase domain protein; citrate lyase ligase 3170698..3171756 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159886 YP_003037229.1 CDS ECBD_3035 NC_012947.1 3171771 3172067 D acyl carrier protein; with CitE and CitF catalyzes the formation of oxaloacetate from citrate; citrate lyase subunit gamma 3171771..3172067 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158826 YP_003037230.1 CDS ECBD_3036 NC_012947.1 3172064 3172972 D KEGG: sbc:SbBS512_E0530 citrate (pro-3S)-lyase, beta subunit; TIGRFAM: citrate lyase subunit beta; PFAM: HpcH/HpaI aldolase; citrate lyase subunit beta 3172064..3172972 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158827 YP_003037231.1 CDS ECBD_3037 NC_012947.1 3172983 3174515 D TIGRFAM: citrate lyase subunit alpha; PFAM: citrate lyase subunit alpha; citrate lyase subunit alpha 3172983..3174515 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158828 YP_003037232.1 CDS citX NC_012947.1 3174519 3175070 D 2'-(5''-phosphoribosyl)-3'-dephospho-CoA transferase; holo-citrate lyase synthase; CitG forms the prosthetic group precursor 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA which is then transferred to apo-ACP by CitX to produce holo-ACP and pyrophosphate; 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA:apo- citrate lyase 3174519..3175070 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158829 YP_003037233.1 CDS citG NC_012947.1 3175045 3175923 D catalyzes the formation of 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA from ATP and 3-dephospho-CoA; triphosphoribosyl-dephospho-CoA synthase 3175045..3175923 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158830 YP_003037234.1 CDS ECBD_3040 NC_012947.1 3175974 3177437 D TIGRFAM: anion transporter; PFAM: sodium/sulfate symporter; anion transporter 3175974..3177437 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158831 YP_003037235.1 CDS ECBD_3041 NC_012947.1 3177551 3178357 D PFAM: ribonuclease T2; ribonuclease I 3177551..3178357 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158832 YP_003037236.1 CDS ECBD_3042 NC_012947.1 3178587 3178997 D Regulates the synthesis of nucleoside triphosphates for nucleic acid synthesis, CTP for lipid synthesis, and GTP for protein elongation; nucleoside diphosphate kinase regulator 3178587..3178997 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158833 YP_003037237.1 CDS ECBD_3043 NC_012947.1 3179036 3179224 D KEGG: sbc:SbBS512_E0523 hypothetical protein; hypothetical protein 3179036..3179224 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158834 YP_003037238.1 CDS ECBD_3044 NC_012947.1 3179228 3180466 R PFAM: alcohol dehydrogenase GroES domain protein; alcohol dehydrogenase zinc-binding domain protein; alcohol dehydrogenase GroES domain protein complement(3179228..3180466) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158835 YP_003037239.1 CDS ECBD_3045 NC_012947.1 3180687 3181115 D PFAM: UspA domain protein; KEGG: sdy:SDY_0542 hypothetical protein; UspA domain protein 3180687..3181115 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158836 YP_003037240.1 CDS ECBD_3046 NC_012947.1 3181236 3182831 R TIGRFAM: alkyl hydroperoxide reductase, F subunit; PFAM: FAD-dependent pyridine nucleotide-disulphide oxidoreductase; KEGG: sbc:SbBS512_E0520 alkyl hydroperoxide reductase subunit F; alkyl hydroperoxide reductase, F subunit complement(3181236..3182831) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158837 YP_003037241.1 CDS ECBD_3047 NC_012947.1 3183046 3183609 R with AhpF catalyzes the conversion of alkyl hydroperoxides to their corresponding alcohols; AhpC reduced the hydroperoxide substrate; alkyl hydroperoxide reductase subunit C complement(3183046..3183609) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158838 YP_003037242.1 CDS dsbG NC_012947.1 3183981 3184742 D Involved in disulfide bond formation; disulfide isomerase/thiol-disulfide oxidase 3183981..3184742 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158839 YP_003037243.1 CDS ECBD_3049 NC_012947.1 3184776 3185051 D PFAM: Insertion element protein; KEGG: dps:DP0861 insertion element iso-IS1d protein (InsA); Insertion element protein 3184776..3185051 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158840 YP_003037244.1 CDS ECBD_3050 NC_012947.1 3184970 3185473 D PFAM: IS1 transposase; KEGG: sty:HCM1.136a IS1 transposase; IS1 transposase 3184970..3185473 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158841 YP_003037245.1 CDS ECBD_3052 NC_012947.1 3186544 3187161 D PFAM: ParB domain protein nuclease; SMART: ParB domain protein nuclease; KEGG: elf:LF82_2590 uncharacterized protein YbdM; ParB domain protein nuclease 3186544..3187161 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158843 YP_003037246.1 CDS ECBD_3053 NC_012947.1 3187162 3188322 R catalyzes the transfer of an amino moiety; aminotransferase complement(3187162..3188322) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158844 YP_003037247.1 CDS ECBD_3054 NC_012947.1 3188431 3189519 D member of the iron-containing alcohol dehydrogenase family; unknown function; hypothetical protein 3188431..3189519 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158845 YP_003037248.1 CDS ECBD_3055 NC_012947.1 3189529 3189726 R PFAM: protein of unknown function DUF466; KEGG: ssn:SSON_0550 hypothetical protein; hypothetical protein complement(3189529..3189726) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158846 YP_003037249.1 CDS ECBD_3057 NC_012947.1 3192184 3192597 R PFAM: thioesterase superfamily protein; KEGG: sfv:SFV_0545 hypothetical protein; hypothetical protein complement(3192184..3192597) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158848 YP_003037250.1 CDS ECBD_3058 NC_012947.1 3192600 3193346 R catalyzes the formation of 2,3-dihydroxybenzoate from 2,3-dihydro-2,3-dihydroxybenzoate; involved in the biosynthesis of siderophores, enterobactin, bacillibactin or vibriobactin; 2,3-dihydroxybenzoate-2,3-dehydrogenase complement(3192600..3193346) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158849 YP_003037251.1 CDS ECBD_3059 NC_012947.1 3193346 3194203 R PFAM: isochorismatase hydrolase; phosphopantetheine-binding; KEGG: sfl:SF0509 2,3-dihydro-2,3-dihydroxybenzoate synthetase, isochroismatase; Isochorismatase complement(3193346..3194203) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158850 YP_003037252.1 CDS entE NC_012947.1 3194217 3195827 R bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate; enterobactin synthase subunit E complement(3194217..3195827) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158851 YP_003037253.1 CDS ECBD_3061 NC_012947.1 3195837 3197012 R TIGRFAM: isochorismate synthase; PFAM: chorismate binding-like; KEGG: sdy:SDY_0524 isochorismate hydroxymutase 2; isochorismate synthase complement(3195837..3197012) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158852 YP_003037254.1 CDS ECBD_3062 NC_012947.1 3197177 3197383 D hypothetical protein 3197177..3197383 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158853 YP_003037255.1 CDS ECBD_3063 NC_012947.1 3197387 3198343 D with FepCDG is involved in the transport of ferric enterobactin; iron-enterobactin transporter periplasmic binding protein 3197387..3198343 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158854 YP_003037256.1 CDS ECBD_3064 NC_012947.1 3198347 3199597 R protein p43; inner membrane protein that exports enterobactin to the periplasmic space; member of the major facilitator superfamily (MFS) of transporters; enterobactin exporter EntS complement(3198347..3199597) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158855 YP_003037257.1 CDS ECBD_3065 NC_012947.1 3199708 3200712 D with FepBCG is involved in the transport of ferric enterobactin; iron-enterobactin transporter membrane protein 3199708..3200712 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158856 YP_003037258.1 CDS ECBD_3066 NC_012947.1 3200709 3201701 D with FepBCD is involved in the transport of ferric enterobactin; iron-enterobactin transporter permease 3200709..3201701 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158857 YP_003037259.1 CDS ECBD_3067 NC_012947.1 3201698 3202513 D with FepBDE is involved in the transport of ferric enterobactin; iron-enterobactin transporter ATP-binding protein 3201698..3202513 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158858 YP_003037260.1 CDS ECBD_3068 NC_012947.1 3202510 3203643 R part of the ferric enterobactin transporter; necessary for enetrobactin uptake; ferric enterobactin transporter FepE complement(3202510..3203643) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158859 YP_003037261.1 CDS entF NC_012947.1 3203859 3207740 R with EntB, EntD, and EntE forms the multienzyme complex enterobactin synthase; EntF is the serine activating enzyme which catalyzes the formation of the amide and ester bonds of the cyclic enterobactin; enterobactin synthase subunit F complement(3203859..3207740) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158860 YP_003037262.1 CDS ECBD_3070 NC_012947.1 3207737 3207955 R PFAM: MbtH domain protein; MbtH domain protein complement(3207737..3207955) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158861 YP_003037263.1 CDS ECBD_3071 NC_012947.1 3207958 3209160 R PFAM: esterase; KEGG: sbc:SbBS512_E0486 enterochelin esterase; enterobactin/ferric enterobactin esterase complement(3207958..3209160) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158862 YP_003037264.1 CDS ECBD_3072 NC_012947.1 3209404 3211644 D Fep; Cbt; Cbr; FeuB; FepA; PfeA; IroN; BfeA; outer membrane receptor of ferric enterobactin and colicins B and D; interacts with the TonB-ExbBD complex which catalyzes the translocation of the siderophore to the periplasmic space; outer membrane receptor FepA 3209404..3211644 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158863 YP_003037265.1 CDS ECBD_3073 NC_012947.1 3211696 3212439 D PFAM: 4'-phosphopantetheinyl transferase; KEGG: sfv:SFV_0528 phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex; phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex 3211696..3212439 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158864 YP_003037266.1 CDS ECBD_3074 NC_012947.1 3212558 3212698 R hypothetical protein complement(3212558..3212698) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158865 YP_003037267.1 CDS ECBD_3075 NC_012947.1 3212721 3213833 R PFAM: transposase IS4 family protein; transposase IS4 family protein complement(3212721..3213833) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158866 YP_003037268.1 CDS ECBD_3076 NC_012947.1 3213910 3214062 R PFAM: Hok/gef cell toxic protein; KEGG: sbc:SbBS512_E0481 Hok/Gef family protein; Hok/gef cell toxic protein complement(3213910..3214062) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158867 YP_003037269.1 CDS ECBD_3078 NC_012947.1 3214338 3214613 D PFAM: Insertion element protein; KEGG: dps:DP0861 insertion element iso-IS1d protein (InsA); Insertion element protein 3214338..3214613 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158869 YP_003037270.1 CDS ECBD_3079 NC_012947.1 3214532 3215035 D PFAM: IS1 transposase; KEGG: sty:HCM1.136a IS1 transposase; IS1 transposase 3214532..3215035 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158870 YP_003037271.1 CDS ECBD_3080 NC_012947.1 3215542 3216063 R KEGG: sfx:S1483 IS103 orf; IS103 orf complement(3215542..3216063) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158871 YP_003037272.1 CDS ECBD_3081 NC_012947.1 3216332 3217450 D ATP-dependent; carboxylate-amine ligase with weak glutamate--cysteine ligase activity; carboxylate-amine ligase 3216332..3217450 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158872 YP_003037273.1 CDS ECBD_3082 NC_012947.1 3217564 3217764 D KEGG: elf:LF82_2587 uncharacterized protein YbdJ; hypothetical protein 3217564..3217764 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158873 YP_003037274.1 CDS ECBD_3083 NC_012947.1 3217829 3218197 D PFAM: protein of unknown function DUF419; KEGG: ssn:SSON_0530 hypothetical protein; hypothetical protein 3217829..3218197 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158874 YP_003037275.1 CDS ECBD_3084 NC_012947.1 3218291 3218944 D catalyzes the reduction of nitroaromatic compounds such as nitrofurazone, quinones and the anti-tumor agent CB1954; NAD(P)H-dependent; oxygen insensitive; dihydropteridine reductase 3218291..3218944 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158875 YP_003037276.1 CDS ECBD_3085 NC_012947.1 3219052 3220299 D PFAM: MscS Mechanosensitive ion channel; KEGG: ssn:SSON_0528 transport; MscS Mechanosensitive ion channel 3219052..3220299 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158876 YP_003037277.1 CDS ECBD_3086 NC_012947.1 3220367 3221779 R PFAM: amino acid permease-associated region; KEGG: sfv:SFV_0516 phenylalanine transporter; phenylalanine transporter complement(3220367..3221779) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158877 YP_003037278.1 CDS ECBD_3087 NC_012947.1 3221845 3224988 R TIGRFAM: heavy metal efflux pump, CzcA family; PFAM: acriflavin resistance protein; heavy metal efflux pump, CzcA family complement(3221845..3224988) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158878 YP_003037279.1 CDS ECBD_3088 NC_012947.1 3225000 3226223 R with CusA, CusC and CusF is part of a cation efflux system that mediates resistance to copper and silver; copper/silver efflux system membrane fusion protein CusB complement(3225000..3226223) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158879 YP_003037280.1 CDS ECBD_3089 NC_012947.1 3226239 3226571 R KEGG: sfv:SFV_0506 periplasmic copper-binding protein; periplasmic copper-binding protein complement(3226239..3226571) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158880 YP_003037281.1 CDS ECBD_3090 NC_012947.1 3226729 3228102 R with CusA, CusB and CusF is part of a cation efflux system that mediates resistance to copper and silver; copper/silver efflux system outer membrane protein CusC complement(3226729..3228102) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158881 YP_003037282.1 CDS ECBD_3091 NC_012947.1 3228259 3228942 D response regulator in two-component regulatory system with CusS; regulates the copper efflux system; CusR family transcriptional regulator 3228259..3228942 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158882 YP_003037283.1 CDS ECBD_3092 NC_012947.1 3228932 3230380 D KEGG: ssn:SSON_0521 sensor kinase CusS; TIGRFAM: heavy metal sensor kinase; PFAM: ATP-binding region ATPase domain protein; histidine kinase HAMP region domain protein; histidine kinase A domain protein; SMART: ATP-binding region ATPase domain protein; histidine kinase A domain protein; histidine kinase HAMP region domain protein; sensor kinase CusS 3228932..3230380 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158883 YP_003037284.1 CDS ECBD_3096 NC_012947.1 3233798 3234373 R PFAM: DNA packaging Nu1; KEGG: ssn:SSON_2431 bacteriophage DNA packaging protein; DNA packaging Nu1 complement(3233798..3234373) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158887 YP_003037285.1 CDS ECBD_3097 NC_012947.1 3234762 3234956 D hypothetical protein 3234762..3234956 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158888 YP_003037286.1 CDS ECBD_3098 NC_012947.1 3235317 3235610 D PFAM: Bor family protein; KEGG: efe:EFER_2699 lipoprotein; DLP12 prophage; Bor family protein 3235317..3235610 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158889 YP_003037287.1 CDS ECBD_3099 NC_012947.1 3235642 3236103 R PFAM: lysis protein; KEGG: elf:LF82_2080 Rz endopeptidase from lambdoid prophage DLP12; lysis protein complement(3235642..3236103) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158890 YP_003037288.1 CDS ECBD_3100 NC_012947.1 3236100 3236597 R PFAM: glycoside hydrolase family 24; Lysozyme complement(3236100..3236597) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158891 YP_003037289.1 CDS ECBD_3101 NC_012947.1 3236597 3236812 R PFAM: Lysis S family protein; Lysis S family protein complement(3236597..3236812) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158892 YP_003037290.1 CDS ECBD_3103 NC_012947.1 3238035 3238310 D PFAM: Insertion element protein; KEGG: dps:DP0861 insertion element iso-IS1d protein (InsA); Insertion element protein 3238035..3238310 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158894 YP_003037291.1 CDS ECBD_3104 NC_012947.1 3238229 3238732 D PFAM: IS1 transposase; KEGG: sty:HCM1.136a IS1 transposase; IS1 transposase 3238229..3238732 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158895 YP_003037292.1 CDS ECBD_3105 NC_012947.1 3239450 3239833 R PFAM: antitermination Q family protein; KEGG: ssn:SSON_2441 hypothetical protein; antitermination Q family protein complement(3239450..3239833) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158896 YP_003037293.1 CDS ECBD_3106 NC_012947.1 3239919 3240056 R KEGG: ssn:SSON_2442 hypothetical protein; hypothetical protein complement(3239919..3240056) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158897 YP_003037294.1 CDS ECBD_3107 NC_012947.1 3240056 3240418 R DLP12 prophage; Holliday junction resolvase; endodeoxyribonuclease RUS complement(3240056..3240418) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158898 YP_003037295.1 CDS ECBD_3108 NC_012947.1 3240415 3240705 R PFAM: protein of unknown function DUF1364; KEGG: elf:LF82_2577 82 prophage-derived uncharacterized protein YbcO; hypothetical protein complement(3240415..3240705) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158899 YP_003037296.1 CDS ECBD_3109 NC_012947.1 3240698 3240868 R DLP12 prophage; conserved protein similar to phage 82 and lambda proteins; prophage protein NinE complement(3240698..3240868) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158900 YP_003037297.1 CDS ECBD_3110 NC_012947.1 3240868 3241323 R DLP12 prophage; hypothetical protein complement(3240868..3241323) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158901 YP_003037298.1 CDS ECBD_3111 NC_012947.1 3241320 3241421 R hypothetical protein complement(3241320..3241421) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158902 YP_003037299.1 CDS ECBD_3112 NC_012947.1 3241538 3242335 R PFAM: helix-turn-helix- domain containing protein AraC type; SMART: helix-turn-helix- domain containing protein AraC type; KEGG: sfv:SFV_1978 regulator; AraC family transcriptional regulator complement(3241538..3242335) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158903 YP_003037300.1 CDS ECBD_3113 NC_012947.1 3242345 3242896 R DLP12 prophage; similar to PEBP/RK1P protein family in eukaryotes that inhibits MEK phosphorylation by Raf-1; crystal structure suggests similar properties but exact function is unknown; kinase inhibitor complement(3242345..3242896) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158904 YP_003037301.1 CDS ECBD_3114 NC_012947.1 3243361 3244887 R PFAM: Recombinase; Resolvase domain; KEGG: elf:LF82_2573 uncharacterized protein YbcK; Recombinase complement(3243361..3244887) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158905 YP_003037302.1 CDS emrE NC_012947.1 3245142 3245474 R member of the small MDR (multidrug resistance) family of transporters; in Escherichia coli this protein provides resistance against a number of positively charged compounds including ethidium bromide and erythromycin; proton-dependent secondary transporter which exchanges protons for compound translocation; multidrug efflux protein complement(3245142..3245474) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158906 YP_003037303.1 CDS ECBD_3118 NC_012947.1 3246256 3246471 D hypothetical protein 3246256..3246471 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156098 YP_003037304.1 CDS ECBD_3119 NC_012947.1 3246570 3246788 D PFAM: zinc finger DksA/TraR C4-type; KEGG: efe:EFER_2731 conserved hypothetical protein from bacteriophage origin; Zn-finger, prokaryotic DksA/TraR C4 type; DnaK suppressor protein; TraR/DksA family transcriptional regulator 3246570..3246788 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156099 YP_003037305.1 CDS ECBD_3120 NC_012947.1 3246836 3247114 D KEGG: efe:EFER_2732 conserved hypothetical protein from phage origin; hypothetical protein 3246836..3247114 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156100 YP_003037306.1 CDS ECBD_3121 NC_012947.1 3247086 3247457 D KEGG: sek:SSPA2268 excisionase; excisionase 3247086..3247457 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156101 YP_003037307.1 CDS ECBD_3122 NC_012947.1 3247313 3248476 D PFAM: integrase; KEGG: sed:SeD_A0603 prophage DLP12 integrase; integrase 3247313..3248476 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156102 YP_003037308.1 CDS ECBD_3123 NC_012947.1 3248811 3249443 D activates the production of the major fimbrae protein FimA; transcriptional regulator FimZ 3248811..3249443 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156104 YP_003037309.1 CDS ECBD_3124 NC_012947.1 3249446 3249961 R PFAM: fimbrial protein; KEGG: ssn:SSON_0507 predicted fimbrial-like adhesin protein; fimbrial protein complement(3249446..3249961) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156105 YP_003037310.1 CDS ECBD_3125 NC_012947.1 3249972 3250949 R P pilus assembly protein pilin FimA-like protein complement(3249972..3250949) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156106 YP_003037311.1 CDS ECBD_3126 NC_012947.1 3250991 3253600 R PFAM: fimbrial biogenesis outer membrane usher protein; fimbrial biogenesis outer membrane usher protein complement(3250991..3253600) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156107 YP_003037312.1 CDS ECBD_3127 NC_012947.1 3253631 3254323 R PFAM: Pili assembly chaperone, N-terminal; Pili assembly chaperone, C-terminal; Pili assembly chaperone, N-terminal complement(3253631..3254323) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156108 YP_003037313.1 CDS ECBD_3128 NC_012947.1 3254543 3255085 R PFAM: fimbrial protein; fimbrial protein complement(3254543..3255085) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156109 YP_003037314.1 CDS ECBD_3129 NC_012947.1 3255556 3256422 D catalyzes the formation of 5,10-methenyltetrahydrofolate from 5,10-methylenetetrahydrofolate and subsequent formation of 10-formyltetrahydrofolate from 5,10-methenyltetrahydrofolate; bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase 3255556..3256422 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156110 YP_003037315.1 CDS ECBD_3130 NC_012947.1 3256424 3256636 D KEGG: sfv:SFV_0486 hypothetical protein; hypothetical protein 3256424..3256636 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156111 YP_003037316.1 CDS ECBD_3131 NC_012947.1 3256744 3257265 D PFAM: membrane-bound metal-dependent hydrolase; KEGG: sbc:SbBS512_E0447 hypothetical protein; membrane-bound metal-dependent hydrolase 3256744..3257265 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156112 YP_003037317.1 CDS cysS NC_012947.1 3257301 3258686 R catalyzes a two-step reaction; charges a cysteine by linking its carboxyl group to the alpha-phosphate of ATP then transfers the aminoacyl-adenylate to its tRNA; cysteinyl-tRNA synthetase complement(3257301..3258686) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156113 YP_003037318.1 CDS ECBD_3133 NC_012947.1 3258860 3259354 D PFAM: peptidyl-prolyl cis-trans isomerase cyclophilin type; KEGG: sfv:SFV_0483 peptidyl-prolyl cis-trans isomerase B (rotamase B); peptidyl-prolyl cis-trans isomerase B (rotamase B) 3258860..3259354 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156114 YP_003037319.1 CDS ECBD_3134 NC_012947.1 3259357 3260079 D catalyzes the formation of 2,3=diacylglucosamine 1-phosphate from UDP-2,3=diacylglucosamine; UDP-2,3-diacylglucosamine hydrolase 3259357..3260079 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156115 YP_003037320.1 CDS ECBD_3135 NC_012947.1 3260197 3260706 D KEGG: sbo:SBO_0420 phosphoribosylaminoimidazole carboxylase; TIGRFAM: phosphoribosylaminoimidazole carboxylase, catalytic subunit; PFAM: 1-(5-phosphoribosyl)-5-amino-4-imidazole-carboxylate (AIR) carboxylase; phosphoribosylaminoimidazole carboxylase, catalytic subunit 3260197..3260706 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156116 YP_003037321.1 CDS ECBD_3136 NC_012947.1 3260703 3261770 D With PurE catalyzes the conversion of aminoimidazole ribonucleotide to carboxyaminoimidazole ribonucleotide in the de novo purine nucleotide biosynthetic pathway; phosphoribosylaminoimidazole carboxylase ATPase subunit 3260703..3261770 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156117 YP_003037322.1 CDS ECBD_3137 NC_012947.1 3261907 3262800 R KEGG: ssn:SSON_0497 carbamate kinase; TIGRFAM: carbamate kinase; PFAM: aspartate/glutamate/uridylate kinase; carbamate kinase complement(3261907..3262800) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156118 YP_003037323.1 CDS ECBD_3138 NC_012947.1 3262797 3263612 R KEGG: sbo:SBO_0423 carboxylase; carboxylase complement(3262797..3263612) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156119 YP_003037324.1 CDS ECBD_3139 NC_012947.1 3263623 3264882 R PFAM: protein of unknown function DUF1116; KEGG: elf:LF82_3525 uncharacterized protein YlbE; hypothetical protein complement(3263623..3264882) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156120 YP_003037325.1 CDS ECBD_3140 NC_012947.1 3264892 3266559 R multicopy suppressor of dominant negative ftsH mutations; membrane protein FdrA complement(3264892..3266559) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156121 YP_003037326.1 CDS ECBD_3141 NC_012947.1 3266876 3267925 D TIGRFAM: ureidoglycolate dehydrogenase; PFAM: malate/L-lactate dehydrogenase; ureidoglycolate dehydrogenase 3266876..3267925 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156122 YP_003037327.1 CDS ECBD_3142 NC_012947.1 3267947 3269182 D allantoate amidohydrolase and N-carbamoyl-L-amino acid amidohydrolase are very similar; the allantoate amidohydrolase from Escherichia coli forms a dimer and binds zinc ions for catalytic activity and catalyzes the conversion of allantoate to (S)-ureidoglycolate and ammonia; carbamoyl amidohydrolase from Bacillus sp. converts N-carbamoyl amino acids to amino acids, ammonia, and carbon dioxide; allantoate amidohydrolase 3267947..3269182 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156123 YP_003037328.1 CDS ECBD_3143 NC_012947.1 3269193 3269978 D TIGRFAM: allantoin catabolism protein; PFAM: protein of unknown function DUF861 cupin_3; Cupin 2 conserved barrel domain protein; KEGG: elf:LF82_3524 uncharacterized protein YlbA; hypothetical protein 3269193..3269978 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156124 YP_003037329.1 CDS ECBD_3144 NC_012947.1 3270206 3271351 R TIGRFAM: glycerate kinase; PFAM: glycerate kinase; glycerate kinase II complement(3270206..3271351) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156125 YP_003037330.1 CDS ECBD_3145 NC_012947.1 3271373 3272674 R PFAM: xanthine/uracil/vitamin C permease; purine permease YbbY complement(3271373..3272674) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156126 YP_003037331.1 CDS ECBD_3146 NC_012947.1 3272731 3274092 R Plays a crucial role on both purine and pyrimidine metabolism; allantoinase complement(3272731..3274092) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156127 YP_003037332.1 CDS ECBD_3147 NC_012947.1 3274152 3275606 R TIGRFAM: NCS1 nucleoside transporter family; PFAM: permease for cytosine/purines uracil thiamine allantoin; allantoin permease complement(3274152..3275606) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156128 YP_003037333.1 CDS ECBD_3148 NC_012947.1 3275775 3276653 R TIGRFAM: 2-hydroxy-3-oxopropionate reductase; PFAM: 6-phosphogluconate dehydrogenase NAD-binding; 2-hydroxy-3-oxopropionate reductase complement(3275775..3276653) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156129 YP_003037334.1 CDS ECBD_3149 NC_012947.1 3276753 3277529 R TIGRFAM: hydroxypyruvate isomerase; PFAM: Xylose isomerase domain protein TIM barrel; hydroxypyruvate isomerase complement(3276753..3277529) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156130 YP_003037335.1 CDS ECBD_3150 NC_012947.1 3277542 3279323 R catalyzes the formation of 2-hydroxy-3-oxopropanoate (tartronate semialdehyde) from two molecules of glyoxylate; glyoxylate carboligase complement(3277542..3279323) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156131 YP_003037336.1 CDS ECBD_3151 NC_012947.1 3279413 3280228 R regulates operons involved in the utilization of allantoin; DNA-binding transcriptional repressor AllR complement(3279413..3280228) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156132 YP_003037337.1 CDS ECBD_3152 NC_012947.1 3280306 3280788 R catalyzes the formation of glyoxylate from (S)-ureidoglycolate; ureidoglycolate hydrolase complement(3280306..3280788) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156133 YP_003037338.1 CDS ECBD_3153 NC_012947.1 3281018 3281944 D activator of the allDC-ylbA operon involved in allantoin utilization; AllS family transcriptional regulator 3281018..3281944 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156134 YP_003037339.1 CDS ECBD_3154 NC_012947.1 3282013 3283107 D catalyzes the selenophosphate-dependent transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA; tRNA 2-selenouridine synthase 3282013..3283107 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156135 YP_003037340.1 CDS ECBD_3155 NC_012947.1 3283223 3283594 D KEGG: sdy:SDY_0400 hypothetical protein; hypothetical protein 3283223..3283594 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156136 YP_003037341.1 CDS ECBD_3156 NC_012947.1 3284338 3284598 R KEGG: sbo:SBO_0403 hypothetical protein; hypothetical protein complement(3284338..3284598) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156137 YP_003037342.1 CDS ECBD_3157 NC_012947.1 3284579 3285289 R PFAM: RHS protein; KEGG: sbo:SBO_0401 protein in rhs element; RHS protein complement(3284579..3285289) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156138 YP_003037343.1 CDS ECBD_3158 NC_012947.1 3285289 3285657 R hypothetical protein complement(3285289..3285657) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156139 YP_003037344.1 CDS ECBD_3159 NC_012947.1 3285697 3289977 R TIGRFAM: hypothetical protein; PFAM: RHS protein; YD repeat-containing protein; hypothetical protein complement(3285697..3289977) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156140 YP_003037345.1 CDS ECBD_3160 NC_012947.1 3290407 3292821 R PFAM: protein of unknown function DUF214; KEGG: ssn:SSON_0485 oxidoreductase; hypothetical protein complement(3290407..3292821) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156141 YP_003037346.1 CDS ECBD_3161 NC_012947.1 3292818 3293504 R PFAM: ABC transporter; SMART: ATPase AAA; KEGG: sbc:SbBS512_E0430 ABC transporter ATP-binding protein; ABC transporter ATP-binding protein complement(3292818..3293504) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156142 YP_003037347.1 CDS ECBD_3162 NC_012947.1 3293472 3294098 D PFAM: lipolytic protein G-D-S-L family; KEGG: efe:EFER_0552 multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1; multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1 3293472..3294098 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156143 YP_003037348.1 CDS ECBD_3163 NC_012947.1 3294127 3294897 D PFAM: short-chain dehydrogenase/reductase SDR; KR domain protein; KEGG: sbc:SbBS512_E0428 oxidoreductase, short chain dehydrogenase/reductase family; short chain dehydrogenase 3294127..3294897 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156144 YP_003037349.1 CDS ECBD_3164 NC_012947.1 3294958 3295812 D PFAM: thioredoxin domain; KEGG: efe:EFER_0550 thioredoxin domain-containing protein; thioredoxin 3294958..3295812 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156145 YP_003037350.1 CDS ECBD_3165 NC_012947.1 3295875 3296654 R PFAM: conserved hypothetical protein; KEGG: ssn:SSON_0480 metal resistance protein; hypothetical protein complement(3295875..3296654) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156146 YP_003037351.1 CDS ECBD_3166 NC_012947.1 3296641 3297318 R PFAM: ABC transporter; SMART: ATPase AAA; KEGG: sdy:SDY_0409 ABC transporter ATP-binding protein YbbL; ABC transporter ATP-binding protein complement(3296641..3297318) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156147 YP_003037352.1 CDS ECBD_3167 NC_012947.1 3297464 3298381 D PFAM: band 7 protein; SMART: band 7 protein; KEGG: sfx:S0441 protease; band 7 protein 3297464..3298381 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156148 YP_003037353.1 CDS ECBD_3168 NC_012947.1 3298378 3298836 D PFAM: protein of unknown function DUF107; KEGG: sbc:SbBS512_E0423 nodulation efficiency family protein; hypothetical protein 3298378..3298836 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156149 YP_003037354.1 CDS ECBD_3169 NC_012947.1 3298837 3299244 R activator of copper-responsive regulon genes; DNA-binding transcriptional regulator CueR complement(3298837..3299244) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156150 YP_003037355.1 CDS ECBD_3170 NC_012947.1 3299369 3300661 R PFAM: amino acid permease-associated region; Spore germination protein; KEGG: sdy:SDY_0432 amino acid/amine transport protein; amino acid permease-associated region complement(3299369..3300661) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156151 YP_003037356.1 CDS ECBD_3171 NC_012947.1 3300664 3301596 R catalyzes the formation of glutamate from glutamine; glutaminase complement(3300664..3301596) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156152 YP_003037357.1 CDS copA NC_012947.1 3301858 3304362 D TIGRFAM: copper-translocating P-type ATPase; heavy metal translocating P-type ATPase; ATPase, P-type (transporting), HAD superfamily, subfamily IC; PFAM: E1-E2 ATPase-associated domain protein; Heavy metal transport/detoxification protein; Haloacid dehalogenase domain protein hydrolase; copper exporting ATPase 3301858..3304362 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156153 YP_003037358.1 CDS ECBD_3173 NC_012947.1 3304779 3305174 R KEGG: sbc:SbBS512_E0418 addiction module antidote protein, HigA family; TIGRFAM: addiction module antidote protein, HigA family; PFAM: helix-turn-helix domain protein; SMART: helix-turn-helix domain protein; XRE family transcriptional regulator complement(3304779..3305174) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156154 YP_003037359.1 CDS ECBD_3174 NC_012947.1 3305258 3306052 D PFAM: GumN family protein; KEGG: sfv:SFV_0455 ligase; GumN family protein 3305258..3306052 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156155 YP_003037360.1 CDS ECBD_3175 NC_012947.1 3306256 3306735 D TIGRFAM: ybaK/ebsC protein; PFAM: YbaK/prolyl-tRNA synthetase associated region; KEGG: sfv:SFV_0454 hypothetical protein; hypothetical protein 3306256..3306735 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156156 YP_003037361.1 CDS ushA NC_012947.1 3306772 3308424 R catalyzes the degradation of periplasmic UDP-glucose to uridine, glucose-1-phosphate and inorganic phosphate; specific for uridine nucleotides; bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor complement(3306772..3308424) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156157 YP_003037362.1 CDS ECBD_3177 NC_012947.1 3308642 3309862 D PFAM: major facilitator superfamily MFS_1; major facilitator superfamily MFS_1 3308642..3309862 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156158 YP_003037363.1 CDS ECBD_3178 NC_012947.1 3310100 3311776 D YbaL; member of the CPA-2 family of antiporters; uncharacterized protein; cation:proton antiport protein 3310100..3311776 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156159 YP_003037364.1 CDS ECBD_3179 NC_012947.1 3311909 3313213 R PFAM: PfkB domain protein; KEGG: ssn:SSON_0464 inosine-guanosine kinase; inosine kinase complement(3311909..3313213) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156160 YP_003037365.1 CDS ECBD_3180 NC_012947.1 3313365 3314324 D PFAM: alpha/beta hydrolase fold-3 domain protein; KEGG: ssn:SSON_0463 acetyl esterase; acetyl esterase 3313365..3314324 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156161 YP_003037366.1 CDS hemH NC_012947.1 3314321 3315283 R protoheme ferro-lyase; catalyzes the insertion of a ferrous ion into protoporphyrin IX to form protoheme; involved in protoheme biosynthesis; in some organisms this protein is membrane-associated while in others it is cytosolic; ferrochelatase complement(3314321..3315283) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156162 YP_003037367.1 CDS adk NC_012947.1 3315519 3316163 R essential enzyme that recycles AMP in active cells; converts ATP and AMP to two molecules of ADP; adenylate kinase complement(3315519..3316163) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156163 YP_003037368.1 CDS ECBD_3183 NC_012947.1 3316344 3318218 R molecular chaperone; heat shock protein 90 complement(3316344..3318218) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156164 YP_003037369.1 CDS recR NC_012947.1 3318328 3318933 R involved in a recombinational process of DNA repair, independent of the recBC complex; recombination protein RecR complement(3318328..3318933) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156165 YP_003037370.1 CDS ECBD_3185 NC_012947.1 3318933 3319262 R PFAM: conserved hypothetical protein; KEGG: sea:SeAg_B0530 hypothetical protein; hypothetical protein complement(3318933..3319262) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156166 YP_003037371.1 CDS ECBD_3186 NC_012947.1 3319315 3321246 R catalyzes the DNA-template-directed extension of the 3'-end of a DNA strand; the tau chain serves as a scaffold to help in the dimerizaton of the alpha,epsilon and theta core complex; the gamma chain seems to interact with the delta and delta' subunits to transfer the beta subunit on the DNA; DNA polymerase III subunits gamma and tau complement(3319315..3321246) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156167 YP_003037372.1 CDS ECBD_3187 NC_012947.1 3321375 3321926 R catalyzes a salvage reaction resulting in the formation of AMP which is metabolically less costly than a de novo synthesis; adenine phosphoribosyltransferase complement(3321375..3321926) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156168 YP_003037373.1 CDS ECBD_3188 NC_012947.1 3322079 3322456 R PFAM: protein of unknown function DUF454; KEGG: sdy:SDY_0451 hypothetical protein; hypothetical protein complement(3322079..3322456) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156169 YP_003037374.1 CDS ECBD_3189 NC_012947.1 3322526 3323053 D PriC; protein involved in DNA replication; part of the primosome, a protein complex required to restart stalled replication forks; binds the complex formed by PriA, PriB and DNA; PriC-dependent primosome requires a gap to restart DNA replication; stimulates Rep activity at stalled forks; primosomal replication protein N'' 3322526..3323053 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156170 YP_003037375.1 CDS ECBD_3190 NC_012947.1 3323067 3323228 D KEGG: sfv:SFV_0439 hypothetical protein; hypothetical protein 3323067..3323228 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156171 YP_003037376.1 CDS ECBD_3191 NC_012947.1 3323440 3326802 R small mechanosensitive ion channel (MscS) that opens in response to stretch forces in the membrane lipid bilayer; maintains cell turgor through accumulation and release of potassium; large protein class of MscS; potassium efflux protein KefA complement(3323440..3326802) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156172 YP_003037377.1 CDS ECBD_3192 NC_012947.1 3326930 3327577 R regulates the acrAB operon which is involved in susceptibility to dephalothin and cephaloridine; DNA-binding transcriptional repressor AcrR complement(3326930..3327577) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156173 YP_003037378.1 CDS ECBD_3193 NC_012947.1 3327719 3328912 D TIGRFAM: efflux transporter RND family, MFP subunit; PFAM: secretion protein HlyD; efflux transporter RND family, MFP subunit 3327719..3328912 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156174 YP_003037379.1 CDS ECBD_3194 NC_012947.1 3328935 3332084 D TIGRFAM: transporter hydrophobe/amphiphile efflux-1 (HAE1) family; PFAM: acriflavin resistance protein; KEGG: elf:LF82_0025 acriflavine resistance protein B; transporter hydrophobe/amphiphile efflux-1 (HAE1) family 3328935..3332084 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156175 YP_003037380.1 CDS ECBD_3195 NC_012947.1 3332630 3333004 D KEGG: ssn:SSON_0448 hypothetical protein; hypothetical protein 3332630..3333004 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156176 YP_003037381.1 CDS ECBD_3196 NC_012947.1 3333030 3333248 D with Hns involved in transcriptional regulation of hemolysin; non-specific DNA-binding protein which affects the production of multiple proteins; hemolysin expression-modulating protein 3333030..3333248 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156177 YP_003037382.1 CDS ECBD_3197 NC_012947.1 3333420 3333971 D PFAM: transferase hexapeptide repeat containing protein; KEGG: sdy:SDY_0460 maltose O-acetyltransferase; maltose O-acetyltransferase 3333420..3333971 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159240 YP_003037383.1 CDS ECBD_3198 NC_012947.1 3334087 3334557 D KEGG: sdy:SDY_0461 hypothetical protein; hypothetical protein 3334087..3334557 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159241 YP_003037384.1 CDS ECBD_3199 NC_012947.1 3334721 3336271 D PFAM: EAL domain protein; SMART: EAL domain protein; KEGG: sfv:SFV_0431 hypothetical protein; EAL domain protein 3334721..3336271 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159242 YP_003037385.1 CDS ECBD_3200 NC_012947.1 3336313 3336666 R PFAM: protein of unknown function DUF1428; KEGG: sdy:SDY_0392 hypothetical protein; hypothetical protein complement(3336313..3336666) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159243 YP_003037386.1 CDS ECBD_3201 NC_012947.1 3337045 3337356 D TIGRFAM: methylated-DNA/protein-cysteine methyltransferase; PFAM: methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding; KEGG: ssn:SSON_0442 hypothetical protein; methylated-DNA/protein-cysteine methyltransferase 3337045..3337356 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159245 YP_003037387.1 CDS ECBD_3202 NC_012947.1 3337387 3337959 R KEGG: sbo:SBO_0348 glycoprotein/polysaccharide metabolism; glycoprotein/polysaccharide metabolism complement(3337387..3337959) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159246 YP_003037388.1 CDS ECBD_3203 NC_012947.1 3338177 3339037 D TIGRFAM: acyl-CoA thioesterase II; PFAM: acyl-CoA thioesterase; KEGG: ssn:SSON_0440 acyl-CoA thioesterase II; acyl-CoA thioesterase II 3338177..3339037 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159247 YP_003037389.1 CDS ECBD_3204 NC_012947.1 3339086 3340372 R TIGRFAM: ammonium transporter; PFAM: Rh family protein/ammonium transporter; KEGG: ssn:SSON_0439 ammonium transporter; ammonium transporter complement(3339086..3340372) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159248 YP_003037390.1 CDS ECBD_3205 NC_012947.1 3340402 3340740 R indirectly regulates nitrogen metabolism; at high nitrogen levels P-II 2 prevents the phosphorylation of NR-I, the transcriptional activator of the glutamine synthetase gene (glnA); at low nitrogen levels P-II 2 is uridylylated to form PII-UMP and interacts with an adenylyltransferase (GlnE) that activates GlnA; functionally it is equivalent to protein P-II (GlnB), but itsexpression is driven by the presence of uridylyltransferase, nitrogen regulator I, and the absence of ammonia.; nitrogen regulatory protein P-II 2 complement(3340402..3340740) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159249 YP_003037391.1 CDS ECBD_3206 NC_012947.1 3340921 3342702 R PFAM: ABC transporter; ABC transporter transmembrane region; SMART: ATPase AAA; KEGG: sbo:SBO_0344 multidrug transporter membrane\ATP-binding components; multidrug transporter membrane\ATP-binding components complement(3340921..3342702) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159250 YP_003037392.1 CDS ECBD_3207 NC_012947.1 3342695 3344467 R PFAM: ABC transporter; ABC transporter transmembrane region; SMART: ATPase AAA; KEGG: sfv:SFV_0422 multidrug transporter membrane\ATP-binding components; multidrug transporter membrane\ATP-binding components complement(3342695..3344467) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159251 YP_003037393.1 CDS ECBD_3208 NC_012947.1 3344497 3344955 R PFAM: regulatory protein AsnC/Lrp family; SMART: regulatory protein AsnC/Lrp family; KEGG: sbc:SbBS512_E0370 transcriptional regulator, AsnC family; AsnC family transcriptional regulator complement(3344497..3344955) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159252 YP_003037394.1 CDS ECBD_3209 NC_012947.1 3345108 3345926 R TIGRFAM: Cof-like hydrolase; HAD-superfamily hydrolase, subfamily IIB; PFAM: haloacid dehalogenase type 3; haloacid dehalogenase; sucrose-6F-phosphate phosphohydrolase; KEGG: sbc:SbBS512_E0369 hydrolase Cof; Cof-like hydrolase complement(3345108..3345926) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159253 YP_003037395.1 CDS ECBD_3210 NC_012947.1 3346026 3347726 D PFAM: extracellular solute-binding protein family 5; KEGG: sbc:SbBS512_E0368 bacterial extracellular solute-binding protein, family 5; extracellular solute-binding protein family 5 3346026..3347726 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159254 YP_003037396.1 CDS ECBD_3211 NC_012947.1 3347791 3348486 D YbaX; catalyzes the transformation of GTP to 7-cyano-7-deazaguanine (preQ0), as one of the early reactions of quenosine biosynthesis; quenosine is a modified nucleoside that occurs at the wobble position of GUN anticodons in tRNAs for Asn, Asp, Tyr, and His; queuosine biosynthesis protein QueC 3347791..3348486 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159255 YP_003037397.1 CDS ECBD_3212 NC_012947.1 3348538 3348936 R PFAM: thioesterase superfamily protein; KEGG: sbc:SbBS512_E0366 thioesterase family protein; thioesterase superfamily protein complement(3348538..3348936) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159256 YP_003037398.1 CDS ECBD_3213 NC_012947.1 3349042 3349413 R TIGRFAM: competence protein ComEA helix-hairpin-helix repeat protein; SMART: DNA-binding protein; KEGG: elf:LF82_2555 uncharacterized protein YbaV; competence protein ComEA helix-hairpin-helix repeat protein complement(3349042..3349413) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159257 YP_003037399.1 CDS ECBD_3214 NC_012947.1 3349564 3351435 R PFAM: PpiC-type peptidyl-prolyl cis-trans isomerase; SurA domain; KEGG: elf:LF82_1706 peptidyl-prolyl cis-trans isomerase D; peptidyl-prolyl cis-trans isomerase (rotamase D) complement(3349564..3351435) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159258 YP_003037400.1 CDS ECBD_3215 NC_012947.1 3351627 3351899 R histone-like DNA-binding protein; transcriptional regulator HU subunit beta complement(3351627..3351899) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159259 YP_003037401.1 CDS ECBD_3216 NC_012947.1 3352108 3354462 R KEGG: elf:LF82_1216 ATP-dependent protease La; TIGRFAM: ATP-dependent protease La; PFAM: peptidase S16; ATPase AAA central domain protein; ATPase associated with various cellular activities AAA_5; SMART: peptidase S16; ATPase AAA; DNA-binding ATP-dependent protease La complement(3352108..3354462) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159260 YP_003037402.1 CDS ECBD_3217 NC_012947.1 3354618 3355730 D PFAM: transposase IS4 family protein; transposase IS4 family protein 3354618..3355730 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159261 YP_003037403.1 CDS ECBD_3218 NC_012947.1 3355753 3355893 D hypothetical protein 3355753..3355893 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159262 YP_003037404.1 CDS clpX NC_012947.1 3356000 3357274 R binds and unfolds substrates as part of the ClpXP protease; ATP-dependent protease ATP-binding subunit ClpX complement(3356000..3357274) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159263 YP_003037405.1 CDS clpP NC_012947.1 3357400 3358023 R hydrolyzes proteins to small peptides; with the ATPase subunits ClpA or ClpX, ClpP degrades specific substrates; ATP-dependent Clp protease proteolytic subunit complement(3357400..3358023) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159264 YP_003037406.1 CDS tig NC_012947.1 3358269 3359567 R Tig; RopA; peptidyl-prolyl cis/trans isomerase; promotes folding of newly synthesized proteins; binds ribosomal 50S subunit; forms a homodimer; trigger factor complement(3358269..3359567) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159265 YP_003037407.1 CDS ECBD_3222 NC_012947.1 3359714 3359902 R KEGG: sdy:SDY_0297 hypothetical protein; hypothetical protein complement(3359714..3359902) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159266 YP_003037408.1 CDS ECBD_3223 NC_012947.1 3359911 3360225 R positive transcriptional regulator of morphogenetic pathway; controlling several genes involved in oxidative stress, acid stress, heat shock, osmotic shock, and carbon-starvation stress; transcriptional regulator BolA complement(3359911..3360225) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159267 YP_003037409.1 CDS ECBD_3224 NC_012947.1 3360533 3361111 D PFAM: Uncharacterized lipoprotein; KEGG: ssn:SSON_0417 hypothetical protein; hypothetical protein 3360533..3361111 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159268 YP_003037410.1 CDS ampG NC_012947.1 3361155 3362630 D in Escherichia coli this protein is a permease involved in peptidoglycan recycling; member of major facilitator superfamily; MFS; inner membrane protein; muropeptide transporter 3361155..3362630 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159269 YP_003037411.1 CDS ECBD_3226 NC_012947.1 3363092 3364039 D TIGRFAM: ubiquinol oxidase subunit II; PFAM: COX aromatic rich domain protein; cytochrome C oxidase subunit II; KEGG: sbo:SBO_0326 cytochrome o ubiquinol oxidase subunit II; cytochrome o ubiquinol oxidase subunit II 3363092..3364039 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159270 YP_003037412.1 CDS ECBD_3227 NC_012947.1 3364061 3366052 D KEGG: ssn:SSON_0414 cytochrome o ubiquinol oxidase subunit I; TIGRFAM: cytochrome o ubiquinol oxidase subunit I; PFAM: cytochrome c oxidase subunit I; cytochrome o ubiquinol oxidase subunit I 3364061..3366052 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159271 YP_003037413.1 CDS ECBD_3228 NC_012947.1 3366042 3366656 D TIGRFAM: cytochrome o ubiquinol oxidase subunit III; PFAM: cytochrome C oxidase subunit III; KEGG: ssn:SSON_0413 cytochrome o ubiquinol oxidase subunit III; cytochrome o ubiquinol oxidase subunit III 3366042..3366656 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159272 YP_003037414.1 CDS ECBD_3229 NC_012947.1 3366656 3366985 D TIGRFAM: cytochrome o ubiquinol oxidase subunit IV; PFAM: cytochrome C oxidase subunit IV; KEGG: sbo:SBO_0323 cytochrome o ubiquinol oxidase subunit IV; cytochrome o ubiquinol oxidase subunit IV 3366656..3366985 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159273 YP_003037415.1 CDS ECBD_3230 NC_012947.1 3366997 3367887 D converts protoheme IX and farnesyl diphosphate to heme O; protoheme IX farnesyltransferase 3366997..3367887 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159274 YP_003037416.1 CDS ECBD_3231 NC_012947.1 3368072 3368383 D PFAM: protein of unknown function DUF1778; KEGG: ssn:SSON_0410 hypothetical protein; hypothetical protein 3368072..3368383 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159275 YP_003037417.1 CDS ECBD_3232 NC_012947.1 3368367 3368909 D PFAM: GCN5-related N-acetyltransferase; KEGG: ssn:SSON_0409 acetyltransferase; GCN5-related N-acetyltransferase 3368367..3368909 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159276 YP_003037418.1 CDS ECBD_3233 NC_012947.1 3368965 3369960 R PFAM: Sel1 domain protein repeat-containing protein; SMART: Sel1 domain protein repeat-containing protein; KEGG: sfv:SFV_0396 hypothetical protein; Sel1 domain protein repeat-containing protein complement(3368965..3369960) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159277 YP_003037419.1 CDS ECBD_3234 NC_012947.1 3370317 3371681 D PFAM: major facilitator superfamily MFS_1; KEGG: ssn:SSON_0404 transporter; major facilitator superfamily MFS_1 3370317..3371681 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159278 YP_003037420.1 CDS ECBD_3235 NC_012947.1 3371809 3372300 R nucleotide binding property based on structural studies of Haemophilus influenzae crystallized protein in PDB Accession Number 1IN0 and NMR studies of Escherichia coli YajQ; the YajQ protein from Pseudomonas synringae appears to play a role in activation of bateriophage phi6 segment L transcription; nucleotide-binding protein complement(3371809..3372300) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159279 YP_003037421.1 CDS ECBD_3236 NC_012947.1 3372468 3373379 D Ketopantoate reductase; catalyzes the NADPH reduction of Ketopantoate to pantoate; functions in pantothenate (vitamin B5) biosynthesis; 2-dehydropantoate 2-reductase 3372468..3373379 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159280 YP_003037422.1 CDS ECBD_3237 NC_012947.1 3373342 3373932 D TIGRFAM: DJ-1 family protein; PFAM: ThiJ/PfpI domain protein; KEGG: sbc:SbBS512_E0346 protein ThiJ; DJ-1 family protein 3373342..3373932 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159281 YP_003037423.1 CDS ECBD_3238 NC_012947.1 3373986 3375434 R Required for the synthesis of the thiazole moiety; thiamine biosynthesis protein ThiI complement(3373986..3375434) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159282 YP_003037424.1 CDS ECBD_3239 NC_012947.1 3375640 3375882 D catalyzes the bidirectional exonucleolytic cleavage of DNA; exodeoxyribonuclease VII small subunit 3375640..3375882 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159283 YP_003037425.1 CDS ECBD_3240 NC_012947.1 3375882 3376781 D PFAM: polyprenyl synthetase; KEGG: sdy:SDY_0309 geranyltranstransferase; geranyltranstransferase 3375882..3376781 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159284 YP_003037426.1 CDS ECBD_3241 NC_012947.1 3376806 3378668 D catalyzes the formation of 1-deoxy-D-xylulose 5-phosphate from pyruvate and D-glyceraldehyde 3-phosphate; 1-deoxy-D-xylulose-5-phosphate synthase 3376806..3378668 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159285 YP_003037427.1 CDS ECBD_3242 NC_012947.1 3378848 3379822 D PFAM: aldo/keto reductase; aldo/keto reductase 3378848..3379822 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159286 YP_003037428.1 CDS ECBD_3243 NC_012947.1 3379876 3380394 R hydrolyzes phosphatidylglycerophosphate to produce phosphatidylglycerol and phosphate; phosphatidylglycerophosphatase A complement(3379876..3380394) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159287 YP_003037429.1 CDS ECBD_3244 NC_012947.1 3380372 3381349 R catalyzes the formation of thiamine diphosphate from thiamine phosphate ant ATP; thiamine monophosphate kinase complement(3380372..3381349) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159288 YP_003037430.1 CDS nusB NC_012947.1 3381427 3381846 R Regulates rRNA biosynthesis by transcriptional antitermination; transcription antitermination protein NusB complement(3381427..3381846) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159289 YP_003037431.1 CDS ribH NC_012947.1 3381866 3382336 R RibE; 6,7-diimethyl-8-ribityllumazine synthase; DMRL synthase; lumazine synthase; subunit beta of riboflavin synthase; condenses 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate to generate 6,6-dimethyl-8-lumazine (DMRL); riboflavin synthase then uses 2 molecules of DMRL to produce riboflavin (vitamin B12); involved in the last steps of riboflavin biosynthesis; forms a 60mer (icosahedral shell) in both Bacillus subtilis and Escherichia coli; in Bacillus subtilis this 60mer is associated with the riboflavin synthase subunit (alpha) while in Escherichia coli it is not; 6,7-dimethyl-8-ribityllumazine synthase complement(3381866..3382336) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159290 YP_003037432.1 CDS ribD NC_012947.1 3382425 3383528 R riboflavin biosynthesis protein which catalyzes the deamination and reduction steps in the riboflavin biosynthesis pathway; catalyzes the formation of 5-amino-6-(5-phosphoribosylamino)uracil from 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine and the formation of 5-amino-6-(5-phosphoribosylamino)uracil from 5-amino-6-(5-phosphoribitylamino)uracil; bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase complement(3382425..3383528) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159291 YP_003037433.1 CDS nrdR NC_012947.1 3383532 3383981 R PFAM: ATP-cone domain protein; KEGG: ssn:SSON_0390 hypothetical protein; NrdR family transcriptional regulator complement(3383532..3383981) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159292 YP_003037434.1 CDS ECBD_3249 NC_012947.1 3384132 3384671 D KEGG: ssn:SSON_0389 hypothetical protein; hypothetical protein 3384132..3384671 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159293 YP_003037435.1 CDS ECBD_3250 NC_012947.1 3384970 3385854 D PFAM: nucleoside-specific channel-forming protein Tsx; KEGG: sfv:SFV_0376 outer membrane protein Tsx; nucleoside-specific channel-forming protein Tsx 3384970..3385854 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159294 YP_003037436.1 CDS ECBD_3251 NC_012947.1 3386031 3386378 R PFAM: HNH endonuclease; SMART: HNH nuclease; KEGG: ssn:SSON_0387 hypothetical protein; hypothetical protein complement(3386031..3386378) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159295 YP_003037437.1 CDS secF NC_012947.1 3386506 3387477 R forms a complex with SecD and YajC; SecDFyajC stimulates the proton motive force-driven protein translocation; seems to modulate the cycling of SecA by stabilizing its membrane-inserted state and appears to be required for the release of mature proteins from the extracytoplasmic side of the membrane; in some organisms, such as Bacillus subtilis, SecD is fused to SecF; preprotein translocase subunit SecF complement(3386506..3387477) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159296 YP_003037438.1 CDS secD NC_012947.1 3387488 3389302 R part of the preprotein secretory system; when complexed with proteins SecF and YajC, SecDFyajC stimulates the proton motive force-driven protein translocation, and appears to be required for the release of mature proteins from the extracytoplasmic side of the membrane; preprotein translocase subunit SecD complement(3387488..3389302) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159297 YP_003037439.1 CDS yajC NC_012947.1 3389363 3389695 R member of preprotein translocase; forms a heterotrimer with SecD and SecF; links the SecD/SecF/YajC/YidC complex with the SecY/SecE/SecG complex; preprotein translocase subunit YajC complement(3389363..3389695) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159298 YP_003037440.1 CDS tgt NC_012947.1 3389718 3390845 R Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr); queuine tRNA-ribosyltransferase complement(3389718..3390845) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159299 YP_003037441.1 CDS queA NC_012947.1 3390901 3391971 R Synthesizes oQ from preQ1 in a single S-adenosylmethionine-requiring step; S-adenosylmethionine:tRNA ribosyltransferase-isomerase complement(3390901..3391971) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159300 YP_003037442.1 CDS ECBD_3257 NC_012947.1 3392064 3392645 D Converts holo-ACP to apo-ACP by hydrolytic cleavage of the phosphopantetheine prosthetic group from ACP; acyl carrier protein phosphodiesterase 3392064..3392645 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159301 YP_003037443.1 CDS ECBD_3258 NC_012947.1 3392650 3394467 R PFAM: alpha amylase catalytic region; glycoside hydrolase family 13 domain protein Ig domain protein region domain protein; SMART: alpha amylase catalytic sub domain; KEGG: ssn:SSON_0380 maltodextrin glucosidase; maltodextrin glucosidase complement(3392650..3394467) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159302 YP_003037444.1 CDS proY NC_012947.1 3394623 3395996 R cryptic permease that may be involved in the transport of proline across the inner membrane; in Salmonella typhimurium, the proY gene is silent unless overexpressed on a multicopy plasmid or activated by a proZ mutation; proline-specific permease complement(3394623..3395996) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159303 YP_003037445.1 CDS ECBD_3260 NC_012947.1 3396072 3397391 R TIGRFAM: branched-chain amino acid transporter II carrier protein; PFAM: branched-chain amino acid transporter II carrier protein; KEGG: sdy:SDY_0336 branched chain amino acid transporter II carrier protein; branched-chain amino acid transporter II carrier protein complement(3396072..3397391) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159304 YP_003037446.1 CDS phoR NC_012947.1 3397798 3399093 R membrane-associated histidine protein kinase that phosphorylates phoB in response to environmental signals as part of the two-component phosphate regulatory system phoR/phoB; phosphate regulon sensor protein complement(3397798..3399093) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159305 YP_003037447.1 CDS ECBD_3262 NC_012947.1 3399151 3399840 R two component response regulator for the phosphate regulon; PhoR phosphorylates PhoB; transcriptional regulator PhoB complement(3399151..3399840) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159306 YP_003037448.1 CDS ECBD_3263 NC_012947.1 3400030 3401232 D with SbcC cleaves DNA hairpin structure, also has 5' single-strand endonuclease activity; exonuclease subunit SbcD 3400030..3401232 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159307 YP_003037449.1 CDS ECBD_3264 NC_012947.1 3401229 3404375 D with SbcD cleaves DNA hairpin structures; also has 5' single-strand endonuclease activity; exonuclease subunit SbcC 3401229..3404375 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159308 YP_003037450.1 CDS ECBD_3265 NC_012947.1 3404417 3405685 D member of the major facilitator superfamily (MFS) of transporters; unknown function; may be associated with transport or processing of arabinose polymers; MFS transporter AraJ 3404417..3405685 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159309 YP_003037451.1 CDS ECBD_3266 NC_012947.1 3405709 3405951 R hypothetical protein complement(3405709..3405951) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159310 YP_003037452.1 CDS ECBD_3267 NC_012947.1 3405930 3406838 R catalyzes phosphorylation of fructose; cytosolic enzyme; fructokinase complement(3405930..3406838) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159311 YP_003037453.1 CDS ECBD_3268 NC_012947.1 3406897 3407874 D PFAM: exonuclease RdgC; KEGG: cko:CKO_02778 recombination associated protein; exonuclease RdgC 3406897..3407874 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159312 YP_003037454.1 CDS ECBD_3269 NC_012947.1 3407944 3408219 D PFAM: Insertion element protein; KEGG: hdu:HD1971 insertion element IS1 1/2/3/5/6 protein InsA; Insertion element protein 3407944..3408219 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159313 YP_003037455.1 CDS ECBD_3270 NC_012947.1 3408138 3408641 D PFAM: IS1 transposase; KEGG: sty:HCM1.136a IS1 transposase; IS1 transposase 3408138..3408641 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159314 YP_003037456.1 CDS ECBD_3271 NC_012947.1 3408676 3408981 D hypothetical protein 3408676..3408981 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159315 YP_003037457.1 CDS ECBD_3272 NC_012947.1 3409012 3409131 D KEGG: sbc:SbBS512_E0309 hypothetical protein; hypothetical protein 3409012..3409131 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159316 YP_003037458.1 CDS ECBD_3273 NC_012947.1 3409298 3409582 R PFAM: protein of unknown function DUF1255; KEGG: ssn:SSON_0367 hypothetical protein; hypothetical protein complement(3409298..3409582) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159317 YP_003037459.1 CDS ECBD_3274 NC_012947.1 3409654 3410331 R PFAM: AroM family protein; KEGG: sbc:SbBS512_E0307 AroM protein; hypothetical protein complement(3409654..3410331) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159318 YP_003037460.1 CDS ECBD_3275 NC_012947.1 3410589 3410780 R KEGG: ssn:SSON_0365 hypothetical protein; hypothetical protein complement(3410589..3410780) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156178 YP_003037461.1 CDS aroL NC_012947.1 3410830 3411354 R type II enzyme similar to type I but differentially regulated and with a lower Km; catalyzes the formation of shikimate 3-phosphate from shikimate in aromatic amino acid biosynthesis; shikimate kinase II complement(3410830..3411354) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156179 YP_003037462.1 CDS ECBD_3277 NC_012947.1 3411537 3411995 R PFAM: protein of unknown function DUF188; KEGG: sdy:SDY_0357 hypothetical protein; hypothetical protein complement(3411537..3411995) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156180 YP_003037463.1 CDS ECBD_3278 NC_012947.1 3412115 3412924 D catalyzes the formation of L-proline from pyrroline-5-carboxylate; pyrroline-5-carboxylate reductase 3412115..3412924 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156181 YP_003037464.1 CDS adrA NC_012947.1 3412941 3414056 R catalyzes the conversion of 2 GTP into c-di-GMP; adrA overexpression induces cellulose biosynthesis, cell adherence to abiotic surfaces and swimming and swarming motility; AdrA acts post-transcriptionally on the bcsABZC operon activating cellulose biosynthesis; diguanylate cyclase AdrA complement(3412941..3414056) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156182 YP_003037465.1 CDS ECBD_3280 NC_012947.1 3414158 3414478 R PFAM: PsiF repeat protein; KEGG: ssn:SSON_0359 hypothetical protein; hypothetical protein complement(3414158..3414478) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156183 YP_003037466.1 CDS ECBD_3281 NC_012947.1 3414597 3416012 R KEGG: sbo:SBO_0278 bacterial alkaline phosphatase; PFAM: alkaline phosphatase; SMART: alkaline phosphatase; alkaline phosphatase complement(3414597..3416012) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156184 YP_003037467.1 CDS ECBD_3282 NC_012947.1 3416113 3416373 R KEGG: ssn:SSON_0357 hypothetical protein; hypothetical protein complement(3416113..3416373) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156185 YP_003037468.1 CDS ddl NC_012947.1 3416836 3417930 D D-alanine--D-alanine ligase; DdlA; DdlB; cytoplasmic; catalyzes the formation of D-alanyl-D-alanine from two D-alanines in peptidoglycan synthesis; there are two forms of this enzyme in Escherichia coli; D-alanyl-alanine synthetase A 3416836..3417930 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156186 YP_003037469.1 CDS ECBD_3284 NC_012947.1 3417954 3418166 R KEGG: sdy:SDY_0366 hypothetical protein; hypothetical protein complement(3417954..3418166) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156187 YP_003037470.1 CDS ECBD_3285 NC_012947.1 3418426 3418734 D KEGG: sbc:SbBS512_E0296 hypothetical protein; hypothetical protein 3418426..3418734 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156188 YP_003037471.1 CDS ECBD_3286 NC_012947.1 3418793 3419887 R PFAM: protein of unknown function DUF1615; KEGG: elf:LF82_2531 uncharacterized protein YaiW; hypothetical protein complement(3418793..3419887) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156189 YP_003037472.1 CDS ECBD_3287 NC_012947.1 3419900 3421120 R in Escherichia coli SbmA is involved in uptake of microcin J25; functions along with FhuA, TonB, and ExbB/D in this capacity; in Sinorhizobium meliloti, BacA is essential and required for symbiosis; defects appear to affect the cell envelope; transporter complement(3419900..3421120) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156190 YP_003037473.1 CDS ECBD_3288 NC_012947.1 3421325 3421426 D hypothetical protein 3421325..3421426 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156191 YP_003037474.1 CDS ECBD_3289 NC_012947.1 3421472 3422629 D this protein has no known enzymatic function; beta-lactam binding protein AmpH 3421472..3422629 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156192 YP_003037475.1 CDS ECBD_3290 NC_012947.1 3422630 3423253 R KEGG: ssn:SSON_0350 DNA-binding transcriptional regulator; DNA-binding transcriptional regulator complement(3422630..3423253) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156193 YP_003037476.1 CDS ECBD_3291 NC_012947.1 3423341 3426247 R TIGRFAM: outer membrane autotransporter barrel domain protein; PFAM: Autotransporter beta- domain protein; Pertactin; KEGG: stm:STM0373 flagellar protein; outer membrane autotransporter barrel domain protein complement(3423341..3426247) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156194 YP_003037477.1 CDS ECBD_3292 NC_012947.1 3426770 3427744 D catalyzes the formation of porphobilinogen from 5-aminolevulinate; delta-aminolevulinic acid dehydratase 3426770..3427744 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156195 YP_003037478.1 CDS tauD NC_012947.1 3427851 3428702 R catalyzes the conversion of taurine (2-aminoethanesulfonate) to sulfite and aminoacetaldehyde concurrently with the conversion of alpha-ketoglutarate to succinate and carbon dioxide; non-heme iron oxidase; allows the use of taurine as an alternative sulfur source; forms homodimers; taurine dioxygenase complement(3427851..3428702) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156196 YP_003037479.1 CDS ECBD_3294 NC_012947.1 3428699 3429526 R PFAM: binding-protein-dependent transporters inner membrane component; KEGG: elf:LF82_2227 taurine transporter permease protein TauC; taurine transporter subunit complement(3428699..3429526) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156197 YP_003037480.1 CDS tauB NC_012947.1 3429523 3430290 R Part of the ABC transporter complex tauABC involved in taurine import; taurine transporter ATP-binding subunit complement(3429523..3430290) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156198 YP_003037481.1 CDS tauA NC_012947.1 3430303 3431265 R with TauB and TauC is responsible for taurine uptake.; taurine transporter substrate binding subunit complement(3430303..3431265) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156199 YP_003037482.1 CDS ECBD_3297 NC_012947.1 3431429 3431704 D PFAM: Insertion element protein; KEGG: dps:DP0861 insertion element iso-IS1d protein (InsA); Insertion element protein 3431429..3431704 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156200 YP_003037483.1 CDS ECBD_3298 NC_012947.1 3431623 3432126 D PFAM: IS1 transposase; KEGG: sty:HCM1.136a IS1 transposase; IS1 transposase 3431623..3432126 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156201 YP_003037484.1 CDS ECBD_3299 NC_012947.1 3432208 3432981 D KEGG: ssn:SSON_0337 hypothetical protein; hypothetical protein 3432208..3432981 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156202 YP_003037485.1 CDS ECBD_3300 NC_012947.1 3433167 3433442 D formaldehyde-induced negative regulator of the frmRAB operon; regulator protein FrmR 3433167..3433442 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156203 YP_003037486.1 CDS ECBD_3301 NC_012947.1 3433477 3434586 D TIGRFAM: S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase; PFAM: alcohol dehydrogenase GroES domain protein; alcohol dehydrogenase zinc-binding domain protein; KEGG: sbc:SbBS512_E0271 S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase; S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase 3433477..3434586 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156204 YP_003037487.1 CDS ECBD_3302 NC_012947.1 3434679 3435512 D KEGG: ssn:SSON_0334 esterase; TIGRFAM: S-formylglutathione hydrolase; PFAM: esterase; S-formylglutathione hydrolase 3434679..3435512 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156205 YP_003037488.1 CDS ECBD_3303 NC_012947.1 3435738 3436277 R hypothetical protein complement(3435738..3436277) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156206 YP_003037489.1 CDS ECBD_3304 NC_012947.1 3436379 3437590 R TIGRFAM: benzoate transport; PFAM: major facilitator superfamily MFS_1; general substrate transporter; 3-hydroxyphenylpropionic transporter MhpT complement(3436379..3437590) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156207 YP_003037490.1 CDS ECBD_3305 NC_012947.1 3437968 3438981 R catalyzes the formation of pyruvate and acetaldehyde from 4-hydroxy-2-ketovaleric acid; involved in the degradation of phenylpropionate; 4-hydroxy-2-ketovalerate aldolase complement(3437968..3438981) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156208 YP_003037491.1 CDS ECBD_3306 NC_012947.1 3438978 3439928 R catalyzes the formation of acetyl-CoA from acetalaldehyde; acetaldehyde dehydrogenase complement(3438978..3439928) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156209 YP_003037492.1 CDS mhpD NC_012947.1 3439925 3440734 R catalyzes the formation of 2-keto-4-hydroxypentanoic acid from 2-hydroxypentadienoic acid; 2-keto-4-pentenoate hydratase complement(3439925..3440734) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156210 YP_003037493.1 CDS ECBD_3308 NC_012947.1 3440744 3441610 R PFAM: alpha/beta hydrolase fold; alpha/beta hydrolase fold protein complement(3440744..3441610) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156211 YP_003037494.1 CDS mhpB NC_012947.1 3441628 3442572 R catalyzes the cleavage of 3-(2,3-dihydroxyphenyl) propionate into 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate; part of the 3-phenylpropionic acid degradation pathway; member of the protocatechuate 4,5-dioxygenase family; 3-(2,3-dihydroxyphenyl)propionate dioxygenase complement(3441628..3442572) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156212 YP_003037495.1 CDS mhpA NC_012947.1 3442574 3444238 R catalyzes the formation of 3-(2,3-dihydroxyphenyl)propionate from 3-(3-hydroxyphenyl)propionate; 3-(3-hydroxyphenyl)propionate hydroxylase complement(3442574..3444238) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156213 YP_003037496.1 CDS ECBD_3311 NC_012947.1 3444417 3445262 D activator of 3-phenylpropionic acid catabolism; DNA-binding transcriptional activator MhpR 3444417..3445262 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156214 YP_003037497.1 CDS lacI NC_012947.1 3445330 3446421 D transcriptional repressor of lac operon; forms a homotetramer as a dimer of dimers; binds specific sites in lac operon resulting in DNA looping between the operators; binds allolactose as inducer; lac repressor 3445330..3446421 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156215 YP_003037498.1 CDS lacZ NC_012947.1 3446544 3449618 D forms a homotetramer; hydrolyzes lactose disaccharide to galactose and glucose; converts lactose to allolactose which is the natural inducer of the lac operon; beta-D-galactosidase 3446544..3449618 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156216 YP_003037499.1 CDS lacY NC_012947.1 3449670 3450923 D lactose/proton symporter; mediates lactose/proton symport through the membrane by utilizing the proton gradient to drive transport of galactosides; member of major facilitator superfamily; functions as a monomer with 12 transmembrane segments; galactoside permease 3449670..3450923 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156217 YP_003037500.1 CDS lacA NC_012947.1 3450980 3451600 D transfers acetyl group from acetyl-CoA to the 6-hydroxyl of galactopyranosides; exact physiological role is unknown; galactoside O-acetyltransferase 3450980..3451600 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156218 YP_003037501.1 CDS cynX NC_012947.1 3451703 3452857 R MFS transporter family member induced in the presence of cyanate as part of a 3 gene operon; not essential; cyanate transporter complement(3451703..3452857) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156219 YP_003037502.1 CDS ECBD_3317 NC_012947.1 3452890 3453360 R catalyzes the reaction of cyanate and bicarbonate to produce ammonia and carbon dioxide; cyanate hydratase complement(3452890..3453360) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156220 YP_003037503.1 CDS ECBD_3318 NC_012947.1 3453391 3454050 R PFAM: carbonic anhydrase; carbonate dehydratase complement(3453391..3454050) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156221 YP_003037504.1 CDS ECBD_3319 NC_012947.1 3454159 3455058 D controls the expression of the cynTSX operon involved in degrading and using cyanate as a sole nitrogen source; DNA-binding transcriptional regulator CynR 3454159..3455058 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156222 YP_003037505.1 CDS ECBD_3320 NC_012947.1 3455395 3456678 R Catalyzes the deamination of cytosine to uracil and ammonia; cytosine deaminase complement(3455395..3456678) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156223 YP_003037506.1 CDS codB NC_012947.1 3456668 3457927 R TIGRFAM: NCS1 nucleoside transporter family; PFAM: permease for cytosine/purines uracil thiamine allantoin; cytosine permease complement(3456668..3457927) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156224 YP_003037507.1 CDS prpE NC_012947.1 3458163 3460049 R catalyzes the formation of propionyl-CoA using propionate as a substrate; PrpE from Ralstonia solanacearum can produce acetyl-, propionyl-, butyryl- and acrylyl-coenzyme A, and Salmonella enterica produces propionyl- and butyryl-coenzyme A; not expressed in Escherichia coli when grown on propionate/minimal media; ATP-dependent; propionyl-CoA synthetase complement(3458163..3460049) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156225 YP_003037508.1 CDS prpD NC_012947.1 3460089 3461540 R functions in propionate metabolism; involved in isomerization of (2S,3S)-methylcitrate to (2R,3S)-methylisocitrate; also encodes minor aconitase or dehydratase activity; aconitase C; 2-methylcitrate dehydratase complement(3460089..3461540) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156226 YP_003037509.1 CDS ECBD_3324 NC_012947.1 3461574 3462743 R catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate; also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity; methylcitrate synthase complement(3461574..3462743) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156227 YP_003037510.1 CDS ECBD_3325 NC_012947.1 3462791 3463237 D KEGG: hypothetical protein; hypothetical protein 3462791..3463237 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156228 YP_003037511.1 CDS prpB NC_012947.1 3463183 3464073 R catalyzes the formation of pyruvate and succinate from 2-methylisocitrate; 2-methylisocitrate lyase complement(3463183..3464073) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156229 YP_003037512.1 CDS ECBD_3327 NC_012947.1 3464312 3465898 D TIGRFAM: propionate catabolism operon regulatory protein PrpR; PFAM: sigma-54 factor interaction domain-containing protein; helix-turn-helix Fis-type; Propionate catabolism activator domain protein; SMART: ATPase AAA; proprionate catabolism activator, Fis family 3464312..3465898 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156230 YP_003037513.1 CDS ECBD_3328 NC_012947.1 3465996 3466271 R PFAM: protein of unknown function DUF1471; hypothetical protein complement(3465996..3466271) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156231 YP_003037514.1 CDS ECBD_3329 NC_012947.1 3466457 3467089 D TIGRFAM: homoserine/threonine efflux pump; PFAM: lysine exporter protein (LYSE/YGGA); homoserine/threonine efflux pump 3466457..3467089 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156232 YP_003037515.1 CDS ECBD_3330 NC_012947.1 3467106 3467351 R KEGG: ssn:SSON_0303 hypothetical protein; hypothetical protein complement(3467106..3467351) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156233 YP_003037516.1 CDS ECBD_3331 NC_012947.1 3467764 3468579 R KEGG: ssn:SSON_0306 hypothetical protein; hypothetical protein complement(3467764..3468579) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156235 YP_003037517.1 CDS ECBD_3332 NC_012947.1 3468823 3469872 R PFAM: alcohol dehydrogenase zinc-binding domain protein; alcohol dehydrogenase GroES domain protein; alcohol dehydrogenase zinc-binding domain protein complement(3468823..3469872) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156236 YP_003037518.1 CDS ECBD_3333 NC_012947.1 3470249 3471631 R metallo-dependent hydrolase superfamily; deaminase with metallo-dependent hydrolase domain; deaminase complement(3470249..3471631) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156237 YP_003037519.1 CDS ECBD_3334 NC_012947.1 3471641 3472591 R PFAM: aspartate/glutamate/uridylate kinase; KEGG: elf:LF82_2518 carbamate kinase-like protein YahI; carbamate kinase complement(3471641..3472591) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156238 YP_003037520.1 CDS ECBD_3335 NC_012947.1 3472667 3472987 R KEGG: hypothetical protein; hypothetical protein complement(3472667..3472987) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156239 YP_003037521.1 CDS ECBD_3336 NC_012947.1 3473013 3474431 R PFAM: protein of unknown function DUF1116; KEGG: elf:LF82_2517 uncharacterized protein YahG; hypothetical protein complement(3473013..3474431) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156240 YP_003037522.1 CDS ECBD_3337 NC_012947.1 3474431 3475978 R PFAM: FdrA family protein; KEGG: ssn:SSON_0312 oxidoreductase subunit; FdrA family protein complement(3474431..3475978) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156241 YP_003037523.1 CDS ECBD_3338 NC_012947.1 3475968 3476831 R KEGG: ssn:SSON_0313 hypothetical protein; hypothetical protein complement(3475968..3476831) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156242 YP_003037524.1 CDS ECBD_3339 NC_012947.1 3476871 3477476 R PFAM: ankyrin; ankyrin complement(3476871..3477476) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156243 YP_003037525.1 CDS ECBD_3340 NC_012947.1 3477734 3478231 D PFAM: protein of unknown function DUF1097; hypothetical protein 3477734..3478231 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156244 YP_003037526.1 CDS ECBD_3341 NC_012947.1 3478323 3479255 D PFAM: LysR substrate-binding; regulatory protein LysR; LysR family transcriptional regulator 3478323..3479255 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156245 YP_003037527.1 CDS ECBD_3342 NC_012947.1 3479297 3480385 R PFAM: EAL domain protein; regulatory protein LuxR; SMART: EAL domain protein; regulatory protein LuxR; KEGG: elf:LF82_2511 uncharacterized protein YahA; diguanylate phosphodiesterase complement(3479297..3480385) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156246 YP_003037528.1 CDS ECBD_3343 NC_012947.1 3480705 3480890 D hypothetical protein 3480705..3480890 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156247 YP_003037529.1 CDS ECBD_3344 NC_012947.1 3481260 3483293 R proton-motive-force-driven choline transporter; choline transporter BetT complement(3481260..3483293) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156248 YP_003037530.1 CDS ECBD_3345 NC_012947.1 3483422 3484009 D HTH-type; bet1; Repressor involved in choline regulation of the bet genes; transcriptional regulator BetI 3483422..3484009 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156249 YP_003037531.1 CDS ECBD_3346 NC_012947.1 3484023 3485495 D catalyzes the formation of betaine from betaine aldehyde; betaine aldehyde dehydrogenase 3484023..3485495 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156250 YP_003037532.1 CDS ECBD_3347 NC_012947.1 3485509 3487179 D catalyzes the oxidation of choline to betaine aldehyde and betain aldehyde to glycine betaine; choline dehydrogenase 3485509..3487179 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156251 YP_003037533.1 CDS ECBD_3348 NC_012947.1 3487392 3488060 D KEGG: sfv:SFV_0321 hypothetical protein; hypothetical protein 3487392..3488060 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156252 YP_003037534.1 CDS ECBD_3349 NC_012947.1 3488303 3488998 R PFAM: protein of unknown function DUF162; KEGG: sbc:SbBS512_E0257 hypothetical protein; hypothetical protein complement(3488303..3488998) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156253 YP_003037535.1 CDS ECBD_3350 NC_012947.1 3488991 3490418 R TIGRFAM: iron-sulfur cluster binding protein; PFAM: protein of unknown function DUF162; KEGG: sfv:SFV_0318 hypothetical protein; iron-sulfur cluster binding protein complement(3488991..3490418) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156254 YP_003037536.1 CDS ECBD_3351 NC_012947.1 3490429 3491148 R PFAM: protein of unknown function DUF224 cysteine-rich region domain protein; KEGG: ssn:SSON_0318 dehydrogenase subunit; hypothetical protein complement(3490429..3491148) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156255 YP_003037537.1 CDS ECBD_3352 NC_012947.1 3491347 3491517 D hypothetical protein 3491347..3491517 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156256 YP_003037538.1 CDS ECBD_3353 NC_012947.1 3491675 3492529 R PFAM: helix-turn-helix- domain containing protein AraC type; SMART: helix-turn-helix- domain containing protein AraC type; AraC family transcriptional regulator complement(3491675..3492529) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160051 YP_003037539.1 CDS ECBD_3354 NC_012947.1 3492755 3494080 D Involved in disulfide oxidoreductase activity and electron transport; pyridine nucleotide-disulfide oxidoreductase 3492755..3494080 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160052 YP_003037540.1 CDS ECBD_3355 NC_012947.1 3494189 3494425 D PFAM: protein of unknown function DUF1471; hypothetical protein 3494189..3494425 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160053 YP_003037541.1 CDS ECBD_3356 NC_012947.1 3494437 3495030 D PFAM: protein of unknown function DUF417; KEGG: ssn:SSON_0322 hypothetical protein; hypothetical protein 3494437..3495030 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160054 YP_003037542.1 CDS ECBD_3357 NC_012947.1 3495621 3496472 D PFAM: transcription activator effector binding; helix-turn-helix- domain containing protein AraC type; SMART: helix-turn-helix- domain containing protein AraC type; KEGG: elf:LF82_3510 uncharacterized HTH-type transcriptional regulator; AraC family transcriptional regulator 3495621..3496472 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160055 YP_003037543.1 CDS ECBD_3358 NC_012947.1 3496612 3497844 R KEGG: elf:LF82_p054 adhesin; hypothetical protein complement(3496612..3497844) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160056 YP_003037544.1 CDS ECBD_3359 NC_012947.1 3498959 3499060 D hypothetical protein 3498959..3499060 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160057 YP_003037545.1 CDS rpmE2 NC_012947.1 3499421 3499687 D RpmE2; there appears to be two types of ribosomal proteins L31 in bacterial genomes; some contain a CxxC motif while others do not; Bacillus subtilis has both types; the proteins in this cluster do not have the CXXC motif; RpmE is found in exponentially growing Bacilli while YtiA was found after exponential growth; expression of ytiA is controlled by a zinc-specific transcriptional repressor; RpmE contains one zinc ion and a CxxC motif is responsible for this binding; forms an RNP particle along with proteins L5, L18, and L25 and 5S rRNA; found crosslinked to L2 and L25 and EF-G; may be near the peptidyltransferase site of the 50S ribosome; 50S ribosomal protein L31 type B 3499421..3499687 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160058 YP_003037546.1 CDS rpmJ NC_012947.1 3499687 3499827 D smallest protein in the large subunit; similar to what is found with protein L31 and L33 several bacterial genomes contain paralogs which may be regulated by zinc; the protein from Thermus thermophilus has a zinc-binding motif and contains a bound zinc ion; the proteins in this group do not have the motif; 50S ribosomal protein L36 3499687..3499827 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160059 YP_003037547.1 CDS ECBD_3362 NC_012947.1 3499862 3500089 R hypothetical protein complement(3499862..3500089) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160060 YP_003037548.1 CDS ECBD_3363 NC_012947.1 3500912 3501454 D PFAM: regulatory protein LuxR; SMART: regulatory protein LuxR; KEGG: sbo:SBO_0225 regulator; LuxR family transcriptional regulator 3500912..3501454 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160061 YP_003037549.1 CDS ECBD_3364 NC_012947.1 3501480 3502115 D KEGG: sbo:SBO_0226 hypothetical protein; hypothetical protein 3501480..3502115 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160062 YP_003037550.1 CDS ECBD_3365 NC_012947.1 3502173 3502841 D KEGG: sbo:SBO_0227 hypothetical protein; hypothetical protein 3502173..3502841 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160063 YP_003037551.1 CDS ECBD_3366 NC_012947.1 3502867 3505392 D hypothetical protein 3502867..3505392 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160064 YP_003037552.1 CDS ECBD_3367 NC_012947.1 3505382 3507025 D KEGG: elf:LF82_2509 uncharacterized protein YagW; hypothetical protein 3505382..3507025 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160065 YP_003037553.1 CDS ECBD_3368 NC_012947.1 3506994 3507704 D hypothetical protein 3506994..3507704 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160066 YP_003037554.1 CDS ECBD_3369 NC_012947.1 3508017 3508346 D PFAM: protein of unknown function UPF0153; KEGG: elf:LF82_3519 uncharacterized protein YkgJ; hypothetical protein 3508017..3508346 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160067 YP_003037555.1 CDS ECBD_3370 NC_012947.1 3508593 3509207 R PFAM: protein of unknown function DUF1440; hypothetical protein complement(3508593..3509207) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160068 YP_003037556.1 CDS ECBD_3371 NC_012947.1 3509624 3510313 D PFAM: [2Fe-2S]-binding domain protein; ferredoxin; KEGG: esa:ESA_04143 hypothetical protein; xanthine dehydrogenase iron-sulfur-binding subunit 3509624..3510313 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160069 YP_003037557.1 CDS ECBD_3372 NC_012947.1 3510310 3511266 D PFAM: molybdopterin dehydrogenase FAD-binding; molybdopterin dehydrogenase FAD-binding 3510310..3511266 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160070 YP_003037558.1 CDS ECBD_3373 NC_012947.1 3511263 3513461 D PFAM: aldehyde oxidase and xanthine dehydrogenase molybdopterin binding; aldehyde oxidase and xanthine dehydrogenase a/b hammerhead; aldehyde oxidase and xanthine dehydrogenase molybdopterin binding 3511263..3513461 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160071 YP_003037559.1 CDS ECBD_3374 NC_012947.1 3513471 3514427 D PFAM: protein of unknown function DUF182; KEGG: esa:ESA_04147 hypothetical protein; hypothetical protein 3513471..3514427 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160072 YP_003037560.1 CDS ECBD_3375 NC_012947.1 3514406 3514816 D KEGG: smd:Smed_5891 LysR family transcriptional regulator; hypothetical protein 3514406..3514816 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160073 YP_003037561.1 CDS proA NC_012947.1 3515133 3516386 R Catalyzes the phosphorylation of L-glutamate during the proline biosynthesis pathway; gamma-glutamyl phosphate reductase complement(3515133..3516386) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160075 YP_003037562.1 CDS ECBD_3377 NC_012947.1 3516398 3517501 R catalyzes the formation of glutamate 5-phosphate from glutamate in proline biosynthesis; gamma-glutamyl kinase complement(3516398..3517501) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160076 YP_003037563.1 CDS ECBD_3378 NC_012947.1 3517789 3518826 D PFAM: porin Gram-negative type; KEGG: sbo:SBO_0247 outer membrane phosphoporin protein E; outer membrane phosphoporin protein E 3517789..3518826 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160077 YP_003037564.1 CDS ECBD_3379 NC_012947.1 3518837 3519340 R PFAM: IS1 transposase; IS1 transposase complement(3518837..3519340) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160078 YP_003037565.1 CDS ECBD_3380 NC_012947.1 3519259 3519534 R PFAM: Insertion element protein; KEGG: hdu:HD1971 insertion element IS1 1/2/3/5/6 protein InsA; Insertion element protein complement(3519259..3519534) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160079 YP_003037566.1 CDS ECBD_3381 NC_012947.1 3519660 3520061 R involved in the expression of csgBA which is involved in curli formation; interacts with sigmaS; DNA-binding transcriptional regulator Crl complement(3519660..3520061) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160080 YP_003037567.1 CDS frsA NC_012947.1 3520119 3521363 R forms a 1:1 complex with the unphosphorylated from of enzyme IIAGlc; FrsA may promote fermentation; fermentation/respiration switch protein complement(3520119..3521363) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160081 YP_003037568.1 CDS ECBD_3383 NC_012947.1 3521455 3521913 R catalyzes the conversion of guanine, xanthine and, to a lesser extent, hypoxanthine to GMP, XMP and IMP, respectively; xanthine-guanine phosphoribosyltransferase complement(3521455..3521913) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160082 YP_003037569.1 CDS ECBD_3384 NC_012947.1 3522174 3523631 D KEGG: sbc:SbBS512_E0234 aminoacyl-histidine dipeptidase; TIGRFAM: aminoacyl-histidine dipeptidase; PFAM: peptidase dimerisation domain protein; peptidase M20; aminoacyl-histidine dipeptidase 3522174..3523631 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160083 YP_003037570.1 CDS prfH NC_012947.1 3523688 3524188 R similar to release factor 1 and 2; peptide chain release factor-like protein complement(3523688..3524188) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160084 YP_003037571.1 CDS ECBD_3386 NC_012947.1 3524157 3524423 R KEGG: sdy:SDY_0482 hypothetical protein; hypothetical protein complement(3524157..3524423) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160085 YP_003037572.1 CDS ECBD_3387 NC_012947.1 3524657 3525109 R PFAM: GCN5-related N-acetyltransferase; KEGG: efe:EFER_2746 acyltransferase with acyl-CoA N-acyltransferase domain; hypothetical protein complement(3524657..3525109) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160086 YP_003037573.1 CDS ECBD_3388 NC_012947.1 3525119 3525517 R YafO overproduction induces toxicity; it is believed to be the toxin of a toxin-antitoxin pair; unknown function; toxin YafO complement(3525119..3525517) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160087 YP_003037574.1 CDS ECBD_3389 NC_012947.1 3525520 3525813 R TIGRFAM: prevent-host-death family protein; PFAM: protein of unknown function DUF172; KEGG: efe:EFER_2748 antitoxin of the YafO-YafN toxin-antitoxin system; antitoxin of the YafO-YafN toxin-antitoxin system complement(3525520..3525813) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160088 YP_003037575.1 CDS ECBD_3390 NC_012947.1 3525865 3526920 R involved in translesion DNA polymerization with beta clamp of polymerase III; belongs to Y family of polymerases; does not contain proofreading function; DNA polymerase IV complement(3525865..3526920) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160089 YP_003037576.1 CDS ECBD_3391 NC_012947.1 3526991 3527626 R PFAM: OmpA/MotB domain protein; KEGG: ssn:SSON_0272 hypothetical protein; hypothetical protein complement(3526991..3527626) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160090 YP_003037577.1 CDS ECBD_3392 NC_012947.1 3527721 3529460 D PFAM: type III secretion FHIPEP protein; KEGG: ssn:SSON_0273 flagellar biosynthesis; type III secretion FHIPEP protein 3527721..3529460 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160091 YP_003037578.1 CDS ECBD_3393 NC_012947.1 3529676 3530173 R KEGG: ssn:SSON_0271 hypothetical protein; hypothetical protein complement(3529676..3530173) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160092 YP_003037579.1 CDS ECBD_3394 NC_012947.1 3530349 3530975 R PFAM: NLP/P60 protein; KEGG: ssn:SSON_0269 lipoprotein; NLP/P60 protein complement(3530349..3530975) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160093 YP_003037580.1 CDS ECBD_3395 NC_012947.1 3531013 3531180 D hypothetical protein 3531013..3531180 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160094 YP_003037581.1 CDS ECBD_3396 NC_012947.1 3531308 3531568 D TIGRFAM: addiction module antitoxin, RelB/DinJ family; PFAM: RelB antitoxin; KEGG: ssn:SSON_0268 damage-inducible protein J; addiction module antitoxin, RelB/DinJ family 3531308..3531568 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160095 YP_003037582.1 CDS ECBD_3397 NC_012947.1 3531571 3531849 D TIGRFAM: addiction module toxin, RelE/StbE family; PFAM: plasmid stabilization system; KEGG: sdy:SDY_0251 hypothetical protein; addiction module toxin, RelE/StbE family 3531571..3531849 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160096 YP_003037583.1 CDS ECBD_3398 NC_012947.1 3532005 3532745 D PFAM: ErfK/YbiS/YcfS/YnhG family protein; KEGG: ssn:SSON_0266 hypothetical protein; ErfK/YbiS/YcfS/YnhG family protein 3532005..3532745 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160097 YP_003037584.1 CDS ECBD_3399 NC_012947.1 3532716 3533483 R PFAM: glutamine amidotransferase class-II; KEGG: sfv:SFV_0305 amidotransferase; glutamine amidotransferase class-II complement(3532716..3533483) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160098 YP_003037585.1 CDS gmhA NC_012947.1 3533689 3534267 R catalyzes the isomerization of sedoheptulose 7-phosphate to D-glycero-D-manno-heptose 7-phosphate; phosphoheptose isomerase complement(3533689..3534267) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160099 YP_003037586.1 CDS fadE NC_012947.1 3534507 3536951 D functions in fatty acid oxidation; converts acyl-CoA and FAD to FADH2 and delta2-enoyl-CoA; acyl-CoA dehydrogenase 3534507..3536951 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160100 YP_003037587.1 CDS ECBD_3402 NC_012947.1 3536994 3537467 R inactivates vertebrate C-type lysozyme; C-lysozyme inhibitor complement(3536994..3537467) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160101 YP_003037588.1 CDS ECBD_3403 NC_012947.1 3537621 3538391 D PFAM: nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase; KEGG: sbc:SbBS512_E0215 hydrolase, carbon-nitrogen family; hypothetical protein 3537621..3538391 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160102 YP_003037589.1 CDS ECBD_3404 NC_012947.1 3538432 3539568 R PFAM: transposase IS4 family protein; KEGG: ssn:SSON_0260 receptor; transposase IS4 family protein complement(3538432..3539568) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160103 YP_003037590.1 CDS ECBD_3405 NC_012947.1 3539999 3540352 R KEGG: sea:SeAg_B4436 hypothetical protein; hypothetical protein complement(3539999..3540352) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160104 YP_003037591.1 CDS ECBD_3406 NC_012947.1 3540372 3540875 R PFAM: IS1 transposase; IS1 transposase complement(3540372..3540875) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160105 YP_003037592.1 CDS ECBD_3407 NC_012947.1 3540794 3541069 R PFAM: Insertion element protein; KEGG: hdu:HD1971 insertion element IS1 1/2/3/5/6 protein InsA; Insertion element protein complement(3540794..3541069) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160106 YP_003037593.1 CDS ECBD_3408 NC_012947.1 3541335 3542120 R KEGG: ssn:SSON_0231 aminopeptidase; aminopeptidase complement(3541335..3542120) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160107 YP_003037594.1 CDS ECBD_3409 NC_012947.1 3542659 3543399 R 3'-5' exonuclease of DNA polymerase III; DNA polymerase III subunit epsilon complement(3542659..3543399) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160109 YP_003037595.1 CDS rnhA NC_012947.1 3543455 3543922 D An endonuclease that specifically degrades the RNA strand of RNA-DNA hybrids; ribonuclease H 3543455..3543922 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160110 YP_003037596.1 CDS ECBD_3411 NC_012947.1 3543919 3544641 R PFAM: methyltransferase type 11; KEGG: sbc:SbBS512_E0210 methyltransferase; methyltransferase type 11 complement(3543919..3544641) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160111 YP_003037597.1 CDS ECBD_3412 NC_012947.1 3544675 3545430 D catalyzes the hydrolysis of S-D-lactoylglutathione to D-lactic acid and reduced glutathione; plays an important role in cellular detoxification using glutathione; hydroxyacylglutathione hydrolase 3544675..3545430 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160112 YP_003037598.1 CDS mltD NC_012947.1 3545502 3546860 D catalyzes the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues; may play a role in recycling muropeptides during cell division and/or cell elongation; in Helicobacter pylori MltD is a endolytic transglycosylase involved mainly in the rearrangement of the peptidoglycan layer of the bacterial cell wall; membrane-bound lytic murein transglycosylase D 3545502..3546860 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160113 YP_003037599.1 CDS ECBD_3414 NC_012947.1 3546908 3547531 R PFAM: methyltransferase type 11; methyltransferase type 12; KEGG: sbc:SbBS512_E0206 methyltransferase, UbiE/COQ5 family; methyltransferase type 11 complement(3546908..3547531) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160114 YP_003037600.1 CDS ECBD_3415 NC_012947.1 3547535 3548314 R PFAM: endonuclease/exonuclease/phosphatase; KEGG: sbc:SbBS512_E0205 endonuclease/exonuclease/phosphatase family protein; hypothetical protein complement(3547535..3548314) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160115 YP_003037601.1 CDS ECBD_3416 NC_012947.1 3548576 3549490 D PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: ssn:SSON_0222 transcriptional regulator LysR-type; LysR family transcriptional regulator 3548576..3549490 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160116 YP_003037602.1 CDS dkgB NC_012947.1 3549487 3550290 R catalyzes the reduction of 2,5-diketo-D-gluconic acid to 2-keto-L-gulonic acid; 2,5-diketo-D-gluconate reductase B complement(3549487..3550290) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160117 YP_003037603.1 CDS ECBD_3418 NC_012947.1 3556138 3556710 R Converts the D-glycero-beta-D-manno-heptose 1,7-bisphosphate intermediate into D-glycero-beta-D-manno-heptose 1-phosphate; D,D-heptose 1,7-bisphosphate phosphatase complement(3556138..3556710) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160124 YP_003037604.1 CDS metN NC_012947.1 3556898 3557929 D part of the metNIQ transporter for methionine; DL-methionine transporter ATP-binding subunit 3556898..3557929 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160125 YP_003037605.1 CDS ECBD_3420 NC_012947.1 3557922 3558575 D part of the MetNIQ methionine uptake system; DL-methionine transporter permease subunit 3557922..3558575 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160126 YP_003037606.1 CDS metQ NC_012947.1 3558615 3559430 D TIGRFAM: lipoprotein, YaeC family; PFAM: NLPA lipoprotein; KEGG: sbc:SbBS512_E0191 D-methionine ABC transporter periplasmic D-methionine-binding lipoprotein; DL-methionine transporter substrate-binding subunit 3558615..3559430 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160127 YP_003037607.1 CDS rcsF NC_012947.1 3559548 3559952 D KEGG: sdy:SDY_0215 outer membrane lipoprotein; outer membrane lipoprotein 3559548..3559952 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160128 YP_003037608.1 CDS ECBD_3423 NC_012947.1 3559949 3560656 D PFAM: protein of unknown function UPF0066; KEGG: ssn:SSON_0209 hypothetical protein; hypothetical protein 3559949..3560656 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160129 YP_003037609.1 CDS ECBD_3424 NC_012947.1 3560767 3562485 D catalyzes the formation of prolyl-tRNA(Pro) from proline and tRNA(Pro); prolyl-tRNA synthetase 3560767..3562485 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156579 YP_003037610.1 CDS ECBD_3425 NC_012947.1 3562538 3563362 D KEGG: sbc:SbBS512_E0187 lipoprotein; hypothetical protein 3562538..3563362 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156580 YP_003037611.1 CDS ECBD_3426 NC_012947.1 3563562 3564272 R PFAM: copper resistance lipoprotein NlpE; KEGG: sbc:SbBS512_E0186 copper homeostasis protein CutF; lipoprotein involved with copper homeostasis and adhesion complement(3563562..3564272) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156581 YP_003037612.1 CDS ECBD_3427 NC_012947.1 3564286 3564708 R PFAM: class I peptide chain release factor; KEGG: sbc:SbBS512_E0185 peptidyl-tRNA hydrolase domain protein; peptidyl-tRNA hydrolase domain protein complement(3564286..3564708) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156582 YP_003037613.1 CDS ECBD_3428 NC_012947.1 3564705 3565250 R PFAM: YaeQ family protein; KEGG: sfv:SFV_0174 hypothetical protein; YaeQ family protein complement(3564705..3565250) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157745 YP_003037614.1 CDS ECBD_3429 NC_012947.1 3565416 3565616 D PFAM: protein of unknown function UPF0253; KEGG: sdy:SDY_0208 hypothetical protein; hypothetical protein 3565416..3565616 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157746 YP_003037615.1 CDS ECBD_3430 NC_012947.1 3565603 3565863 D Suppresses temperature-sensitive mutations in essential genes by modulating rho-dependent transcription termination; Rho-binding antiterminator 3565603..3565863 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157747 YP_003037616.1 CDS tilS NC_012947.1 3565912 3567210 R Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU; ATP-dependent; responsible for modifying the wobble-base of the CAU anticodon of tRNAIle such that it exhibits proper recognition of the AUA codon rather than the AUG codon and is in turn properly recognized by isoleucyl-tRNA synthetase; tRNA(Ile)-lysidine synthetase complement(3565912..3567210) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157748 YP_003037617.1 CDS ECBD_3432 NC_012947.1 3567275 3567664 R PFAM: glyoxalase/bleomycin resistance protein/dioxygenase; KEGG: sbc:SbBS512_E0180 glyoxylase family protein; hypothetical protein complement(3567275..3567664) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157749 YP_003037618.1 CDS ECBD_3433 NC_012947.1 3567721 3569862 R PFAM: Orn/Lys/Arg decarboxylase major region; Orn/Lys/Arg decarboxylase domain protein; KEGG: ssn:SSON_0199 lysine decarboxylase 2, constitutive; lysine decarboxylase complement(3567721..3569862) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157750 YP_003037619.1 CDS ECBD_3434 NC_012947.1 3569961 3570920 R catalyzes the carboxylation of acetyl-CoA to malonyl-CoA; forms a tetramer composed of two alpha (AccA) and two beta (AccD) subunits; one of the two catalytic subunits that can form the acetyl CoA carboxylase enzyme together with a carrier protein; acetyl-CoA carboxylase carboxyltransferase subunit alpha complement(3569961..3570920) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157751 YP_003037620.1 CDS dnaE NC_012947.1 3570933 3574415 R catalyzes DNA-template-directed extension of the 3'- end of a DNA strand by one nucleotide at a time; main replicative polymerase; DNA polymerase III subunit alpha complement(3570933..3574415) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157752 YP_003037621.1 CDS rnhB NC_012947.1 3574452 3575048 R RNH2; RNase HII; binds manganese; endonuclease which specifically degrades the RNA of RNA-DNA hybrids; ribonuclease HII complement(3574452..3575048) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157753 YP_003037622.1 CDS lpxB NC_012947.1 3575045 3576193 R catalyzes the formation of lipid A disaccharide from UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate, lipid A disaccharide is a precursor of lipid A that anchors LPS to the OM; lipid-A-disaccharide synthase complement(3575045..3576193) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157754 YP_003037623.1 CDS ECBD_3438 NC_012947.1 3576193 3576981 R catalyzes the addition of (R)-3-hydroxytetradecanoyl to the glucosamine disaccharide in lipid A biosynthesis; UDP-N-acetylglucosamine acyltransferase complement(3576193..3576981) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157755 YP_003037624.1 CDS fabZ NC_012947.1 3576985 3577440 R in Pseudomonas aeruginosa this enzyme is a trimer of dimers; essential for membrane formation; performs third step of type II fatty acid biosynthesis; catalyzes dehydration of (3R)-hydroxyacyl-ACP to trans-2-acyl-ACP; (3R)-hydroxymyristoyl-ACP dehydratase complement(3576985..3577440) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157756 YP_003037625.1 CDS lpxD NC_012947.1 3577545 3578570 R adds the O-linked and N-linked 3(R)-hydroxy fatty acids to the glucosamine disaccharide during lipid A biosynthesis; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase complement(3577545..3578570) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157757 YP_003037626.1 CDS ECBD_3441 NC_012947.1 3578574 3579059 R PFAM: outer membrane chaperone Skp (OmpH); KEGG: ssn:SSON_0190 periplasmic chaperone; periplasmic chaperone complement(3578574..3579059) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157758 YP_003037627.1 CDS ECBD_3442 NC_012947.1 3579181 3581613 R part of a complex with YfgL, YfiO, and NlpB involved in outer membrane protein biosynthesis; involved in the assembly of outer membrane proteins; outer membrane protein assembly factor YaeT complement(3579181..3581613) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157759 YP_003037628.1 CDS ECBD_3443 NC_012947.1 3581643 3582995 R catalyzes the cleavage of RseA which activates the sigmaE-mediated stress response; zinc metallopeptidase RseP complement(3581643..3582995) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157760 YP_003037629.1 CDS cdsA NC_012947.1 3583007 3583864 R catalyzes the synthesis of CDP-diglyceride from CTP and phosphatidate; CDP-diglyceride synthase complement(3583007..3583864) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157761 YP_003037630.1 CDS ECBD_3445 NC_012947.1 3583877 3584635 R catalyzes the formation of undecaprenyl pyrophosphate from isopentenyl pyrophosphate; UDP pyrophosphate synthase complement(3583877..3584635) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157762 YP_003037631.1 CDS ECBD_3446 NC_012947.1 3584824 3586020 R catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate; 1-deoxy-D-xylulose 5-phosphate reductoisomerase complement(3584824..3586020) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157763 YP_003037632.1 CDS frr NC_012947.1 3586112 3586669 R Rrf; Frr; ribosome-recycling factor; release factor 4; RF4; recycles ribosomes upon translation termination along with release factor RF-3 and elongation factor EF-G; A GTPase-dependent process results in release of 50S from 70S; inhibited by release factor RF-1; essential for viability; structurally similar to tRNAs; ribosome recycling factor complement(3586112..3586669) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157764 YP_003037633.1 CDS pyrH NC_012947.1 3586961 3587686 R Catalyzes the phosphorylation of UMP to UDP; uridylate kinase complement(3586961..3587686) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157765 YP_003037634.1 CDS tsf NC_012947.1 3587833 3588684 R EF-Ts; functions during elongation stage of protein translation; forms a dimer; associates with EF-Tu-GDP complex and promotes exchange of GDP to GTP resulting in regeneration of the active form of EF-Tu; elongation factor Ts complement(3587833..3588684) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157766 YP_003037635.1 CDS rpsB NC_012947.1 3588942 3589667 R one of the last subunits in the assembly of the 30S subunit; absence of S2 does not inhibit assembly but results in an inactive subunit; 30S ribosomal protein S2 complement(3588942..3589667) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157767 YP_003037636.1 CDS ECBD_3451 NC_012947.1 3590035 3590829 D catalyzes the removal of N-terminal amino acids from peptides and arylamides; generally Co(II) however activity has been shown for some methionine aminopeptidases with Zn, Fe, or Mn; methionine aminopeptidase 3590035..3590829 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157768 YP_003037637.1 CDS ECBD_3452 NC_012947.1 3590891 3593563 D catalyzes the uridylylation or deuridylylation of the PII nitrogen regulatory protein; PII uridylyl-transferase 3590891..3593563 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157769 YP_003037638.1 CDS dapD NC_012947.1 3593594 3594418 D catalyzes the formation of N-succinyl-2-amino-6-ketopimelate from succinyl-CoA and tetrahydrodipicolinate in the lysine biosynthetic pathway; 2,3,4,5-tetrahydropyridine-2,6-carboxylate N-succinyltransferase 3593594..3594418 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157770 YP_003037639.1 CDS ECBD_3454 NC_012947.1 3594472 3595284 D PFAM: metallophosphoesterase; KEGG: sbo:SBO_0153 phosphodiesterase YaeI; phosphodiesterase YaeI 3594472..3595284 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157771 YP_003037640.1 CDS ECBD_3455 NC_012947.1 3595446 3595832 D KEGG: ssn:SSON_0176 structural protein; hypothetical protein 3595446..3595832 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157772 YP_003037641.1 CDS ECBD_3456 NC_012947.1 3595921 3597078 R regulates the expression of the operons for the enzymes involved in galactarate, glucarate and glycerate utilization; carbohydrate diacid transcriptional activator CdaR complement(3595921..3597078) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157773 YP_003037642.1 CDS ECBD_3457 NC_012947.1 3597233 3598657 R protease Do; required at high temperature; degrades damaged proteins; serine endoprotease complement(3597233..3598657) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157774 YP_003037643.1 CDS dgt NC_012947.1 3598787 3600304 R forms a homotetramer; requires magnesium for activity; catalyzes the hydrolysis of dGTP to form deoxyguanosine and triphosphate; deoxyguanosinetriphosphate triphosphohydrolase complement(3598787..3600304) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157775 YP_003037644.1 CDS ECBD_3459 NC_012947.1 3600388 3601086 D enables the cleavage of the glycosidic bond in both 5'-methylthioadenosine and S-adenosylhomocysteine; 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 3600388..3601086 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157776 YP_003037645.1 CDS ECBD_3460 NC_012947.1 3601079 3601879 D solute binding component of the vitamin B12 transport system BtuCDF; vitamin B12-transporter protein BtuF 3601079..3601879 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157777 YP_003037646.1 CDS ECBD_3461 NC_012947.1 3601917 3602540 D PFAM: protein of unknown function UPF0126; KEGG: sbc:SbBS512_E0149 hypothetical protein; hypothetical protein 3601917..3602540 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157778 YP_003037647.1 CDS ECBD_3462 NC_012947.1 3602587 3602931 R essential respiratory protein A; may be involved in the transfer of iron-sulfur clusters; essential for growth using oxygen or alternate electron acceptors; iron-sulfur cluster insertion protein ErpA complement(3602587..3602931) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157779 YP_003037648.1 CDS ECBD_3463 NC_012947.1 3603013 3604434 R Acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation; chloride channel protein complement(3603013..3604434) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157780 YP_003037649.1 CDS ECBD_3464 NC_012947.1 3604659 3605939 D Converts (S)-4-amino-5-oxopentanoate to 5-aminolevulinate during the porphyrin biosynthesis pathway; glutamate-1-semialdehyde aminotransferase 3604659..3605939 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157781 YP_003037650.1 CDS ECBD_3465 NC_012947.1 3606097 3608079 R part of the FhuBCD ATP-dependent iron (III) hydroxamate transporter involved in the high-affinity transport of Fe(3+)-ferrichrome; iron-hydroxamate transporter permease subunit complement(3606097..3608079) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157782 YP_003037651.1 CDS ECBD_3466 NC_012947.1 3608076 3608966 R Part of the FhuBCD ATP-dependent iron (III) hydroxamate transporter; binds to all hydroxamate siderophores; iron-hydroxamate transporter substrate-binding subunit complement(3608076..3608966) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157783 YP_003037652.1 CDS ECBD_3467 NC_012947.1 3608966 3609763 R part of the FhuBCD ATP-dependent iron (III) hydroxamate transporter; iron-hydroxamate transporter ATP-binding subunit complement(3608966..3609763) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157784 YP_003037653.1 CDS ECBD_3469 NC_012947.1 3610918 3612126 R PFAM: transposase IS4 family protein; KEGG: seh:SeHA_C4716 transposase (IS4 family); transposase IS4 family protein complement(3610918..3612126) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157786 YP_003037654.1 CDS mrcB NC_012947.1 3613615 3616140 R bifunctional periplasmic enzyme; contains transglycosylase and transpeptidase activity; major enzyme for peptidoglycan biosynthesis in Escherichia coli; transmembrane protein; forms dimers; three variants, one of which may be a degradation product, while the other appears to result from an alternative initiation site, are found within the cell; penicillin-binding protein 1b complement(3613615..3616140) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157787 YP_003037655.1 CDS ECBD_3471 NC_012947.1 3616336 3618765 R similar in sequence to the ATP-dependent RNA helicase HrpA; ATP-dependent RNA helicase HrpB complement(3616336..3618765) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157788 YP_003037656.1 CDS ECBD_3472 NC_012947.1 3618839 3619369 D TIGRFAM: 2'-5' RNA ligase; PFAM: phosphoesterase HXTX; KEGG: ssn:SSON_0159 hypothetical protein; 2'-5' RNA ligase 3618839..3619369 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157789 YP_003037657.1 CDS ECBD_3473 NC_012947.1 3619384 3620088 D Regulatory factor involved in maltose metabolism; sugar fermentation stimulation protein A 3619384..3620088 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157790 YP_003037658.1 CDS dksA NC_012947.1 3620266 3620721 D TIGRFAM: RNA polymerase-binding protein DksA; PFAM: zinc finger DksA/TraR C4-type; KEGG: sdy:SDY_0161 DnaK transcriptional regulator DksA; RNA polymerase-binding transcription factor 3620266..3620721 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157791 YP_003037659.1 CDS ECBD_3475 NC_012947.1 3620758 3621684 D this tRNA synthetase lacks the tRNA anticodon interaction domain; instead this enzyme modifies tRNA(Asp) with glutamate by esterifying glutamate to the 2-amino-5-(4,5-dihydroxy-2-cyclopenten-1-yl) moiety of queosine generating a modified nucleoside at the first anticodon position of tRNAAsp; the modified tRNA does not bind elongation factor Tu; glutamyl-Q tRNA(Asp) synthetase 3620758..3621684 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157792 YP_003037660.1 CDS pcnB NC_012947.1 3621723 3623141 D polymerase that creates the 3' poly(A) tail found in some mRNA's; poly(A) polymerase I 3621723..3623141 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157793 YP_003037661.1 CDS ECBD_3477 NC_012947.1 3623138 3623617 D catalyzes the formation of 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine and ATP; 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase 3623138..3623617 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157794 YP_003037662.1 CDS ECBD_3478 NC_012947.1 3623987 3624571 D PFAM: fimbrial protein; KEGG: ssn:SSON_0153 fimbrial-like protein; fimbrial protein 3623987..3624571 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157795 YP_003037663.1 CDS ECBD_3479 NC_012947.1 3624676 3625416 D PFAM: Pili assembly chaperone, N-terminal; Pili assembly chaperone, C-terminal; KEGG: sbo:SBO_0126 chaperone protein EcpD; chaperone protein EcpD 3624676..3625416 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157796 YP_003037664.1 CDS ECBD_3480 NC_012947.1 3625451 3628051 D similar to the fimbrial usher protein PapC; outer membrane usher protein 3625451..3628051 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157797 YP_003037665.1 CDS ECBD_3481 NC_012947.1 3628068 3628637 D KEGG: efe:EFER_0160 fimbrial-like adhesin exported protein; fimbrial-like adhesin exported protein 3628068..3628637 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157798 YP_003037666.1 CDS ECBD_3482 NC_012947.1 3628652 3629254 D PFAM: fimbrial protein; KEGG: ssn:SSON_0146 fimbrial protein; fimbrial protein 3628652..3629254 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157799 YP_003037667.1 CDS ECBD_3483 NC_012947.1 3629281 3629877 D KEGG: ssn:SSON_0145 fimbrial protein; fimbrial protein 3629281..3629877 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157800 YP_003037668.1 CDS ECBD_3484 NC_012947.1 3629930 3631204 D PFAM: fimbrial protein; KEGG: elf:LF82_2474 uncharacterized fimbrial-like protein YadC; fimbrial-like adhesin protein 3629930..3631204 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157801 YP_003037669.1 CDS panB NC_012947.1 3631317 3632111 D catalyzes the formation of tetrahydrofolate and 2-dehydropantoate from 5,10-methylenetetrahydrofolate and 3-methyl-2-oxobutanoate; 3-methyl-2-oxobutanoate hydroxymethyltransferase 3631317..3632111 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157802 YP_003037670.1 CDS panC NC_012947.1 3632123 3632974 D catalyzes the formation of (R)-pantothenate from pantoate and beta-alanine; pantoate--beta-alanine ligase 3632123..3632974 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157803 YP_003037671.1 CDS ECBD_3487 NC_012947.1 3633048 3633950 R PFAM: transposase YhgA family protein; KEGG: sfv:SFV_0122 hypothetical protein; transposase YhgA family protein complement(3633048..3633950) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157804 YP_003037672.1 CDS ECBD_3488 NC_012947.1 3634224 3634604 D Converts L-aspartate to beta-alanine and provides the major route of beta-alanine production in bacteria. beta-alanine is essential for the biosynthesis of pantothenate (vitamin B5); aspartate alpha-decarboxylase 3634224..3634604 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157805 YP_003037673.1 CDS ECBD_3489 NC_012947.1 3634608 3635837 R PFAM: polysaccharide deacetylase; KEGG: ssn:SSON_0138 hypothetical protein; polysaccharide deacetylase complement(3634608..3635837) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157806 YP_003037674.1 CDS ECBD_3490 NC_012947.1 3635901 3636341 R PFAM: PTS system fructose subfamily IIA component; KEGG: sbc:SbBS512_E0122 PTS system IIA component domain protein; PTS system fructose subfamily IIA component complement(3635901..3636341) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157807 YP_003037675.1 CDS ECBD_3491 NC_012947.1 3636446 3637216 R PFAM: ABC transporter; KEGG: ssn:SSON_0136 hypothetical protein; ABC transporter complement(3636446..3637216) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157808 YP_003037676.1 CDS ECBD_3492 NC_012947.1 3637213 3638139 R PFAM: ABC transporter; SMART: ATPase AAA; KEGG: ssn:SSON_0135 ATP-binding component of a transporter; ABC transporter complement(3637213..3638139) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157809 YP_003037677.1 CDS ECBD_3493 NC_012947.1 3638248 3638910 D catalyzes the interconversion of bicarbonate and carbon dioxide; carbonic anhydrase 3638248..3638910 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157810 YP_003037678.1 CDS ECBD_3494 NC_012947.1 3638951 3639499 R KEGG: sfv:SFV_0116 hypoxanthine-guanine phosphoribosyltransferase; TIGRFAM: hypoxanthine phosphoribosyltransferase; PFAM: phosphoribosyltransferase; hypoxanthine phosphoribosyltransferase complement(3638951..3639499) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157811 YP_003037679.1 CDS ECBD_3495 NC_012947.1 3639693 3642083 D TIGRFAM: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family; PFAM: pyrrolo-quinoline quinone; SMART: pyrrolo-quinoline quinone; membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family 3639693..3642083 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157812 YP_003037680.1 CDS ECBD_3496 NC_012947.1 3642285 3643835 R laccase; copper-stimulated phenoloxidase and ferroxidase which may be involved in copper detoxification; multicopper oxidase complement(3642285..3643835) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157813 YP_003037681.1 CDS ECBD_3497 NC_012947.1 3644001 3644348 D KEGG: ssn:SSON_0130 hypothetical protein; hypothetical protein 3644001..3644348 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157814 YP_003037682.1 CDS ECBD_3498 NC_012947.1 3644454 3645320 D catalyzes the formation of spermidine from putrescine and S-adenosylmethioninamine; spermidine synthase 3644454..3645320 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157815 YP_003037683.1 CDS ECBD_3499 NC_012947.1 3645336 3646130 D S-adenosylmethionine provides the aminopropyl moiety required for spermidine biosynthesis from putrescine; S-adenosylmethionine decarboxylase 3645336..3646130 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157816 YP_003037684.1 CDS ECBD_3500 NC_012947.1 3646168 3646530 R PFAM: conserved hypothetical protein; KEGG: ssn:SSON_0127 hypothetical protein; hypothetical protein complement(3646168..3646530) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157817 YP_003037685.1 CDS ECBD_3501 NC_012947.1 3646705 3649302 R catalyzes the conversion of citrate to isocitrate and the conversion of 2-methylaconitate to 2-methylisocitrate; bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase complement(3646705..3649302) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157818 YP_003037686.1 CDS ECBD_3502 NC_012947.1 3649657 3651510 D KEGG: sbc:SbBS512_E0110 hypothetical protein; hypothetical protein 3649657..3651510 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157819 YP_003037687.1 CDS ECBD_3503 NC_012947.1 3651581 3653005 R E3 component of pyruvate and 2-oxoglutarate dehydrogenase complex; catalyzes the oxidation of dihydrolipoamide to lipoamide; dihydrolipoamide dehydrogenase complement(3651581..3653005) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157820 YP_003037688.1 CDS aceF NC_012947.1 3653330 3655222 R E2 component of pyruvate dehydrogenase multienzyme complex; in Escherichia coli AceF contains three N-terminal lipoyl domains; dihydrolipoamide acetyltransferase complement(3653330..3655222) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157821 YP_003037689.1 CDS aceE NC_012947.1 3655237 3657900 R E1 component; part of pyruvate dehydrogenase; forms a complex with DlaT and LpdC; pyruvate dehydrogenase subunit E1 complement(3655237..3657900) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157822 YP_003037690.1 CDS pdhR NC_012947.1 3658061 3658825 R activates lctPRD operon; autoregulates itself through repression of pdhR-aceEF-lpdA operon; regulates pyruvate dehydrogenase complex; transcriptional regulator PdhR complement(3658061..3658825) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157823 YP_003037691.1 CDS ECBD_3507 NC_012947.1 3659369 3660739 D PFAM: amino acid permease-associated region; KEGG: efe:EFER_0132 aromatic amino acid transporter; aromatic amino acid transporter 3659369..3660739 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157824 YP_003037692.1 CDS ECBD_3508 NC_012947.1 3660782 3661636 R involved in regulation of beta-lactamase; signaling protein; regulatory protein AmpE complement(3660782..3661636) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156583 YP_003037693.1 CDS ECBD_3509 NC_012947.1 3661633 3662184 R PFAM: N-acetylmuramoyl-L-alanine amidase family 2; SMART: N-acetylmuramoyl-L-alanine amidase family 2; N-acetyl-anhydromuranmyl-L-alanine amidase complement(3661633..3662184) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8155934 YP_003037694.1 CDS ECBD_3510 NC_012947.1 3662272 3663165 D catalyzes the formation of pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose 1-diphosphate from nictinate D-ribonucleotide; quinolinate phosphoribosyltransferase 3662272..3663165 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156584 YP_003037695.1 CDS ECBD_3511 NC_012947.1 3663368 3663808 D KEGG: ssn:SSON_0116 major pilin subunit; major pilin subunit 3663368..3663808 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156585 YP_003037696.1 CDS ECBD_3512 NC_012947.1 3663818 3665203 D PFAM: type II secretion system protein E; hypothetical protein 3663818..3665203 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158155 YP_003037697.1 CDS ECBD_3513 NC_012947.1 3665193 3666395 D PFAM: type II secretion system protein; KEGG: sbc:SbBS512_E0098 type IV pilus assembly protein PilC; type IV pilin biogenesis protein 3665193..3666395 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158243 YP_003037698.1 CDS ECBD_3514 NC_012947.1 3666430 3667473 R catalyzes the NADPH-dependent deamination of GMP to inosine monophosphate; guanosine 5'-monophosphate oxidoreductase complement(3666430..3667473) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158244 YP_003037699.1 CDS coaE NC_012947.1 3667698 3668318 D catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A; involved in coenzyme A biosynthesis; dephospho-CoA kinase 3667698..3668318 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158245 YP_003037700.1 CDS ECBD_3516 NC_012947.1 3668318 3669061 D PFAM: protein of unknown function DUF1342; KEGG: ssn:SSON_0110 hypothetical protein; hypothetical protein 3668318..3669061 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158246 YP_003037701.1 CDS ECBD_3517 NC_012947.1 3669071 3669268 D PFAM: protein of unknown function DUF329; KEGG: ssn:SSON_0109 zinc-binding protein; zinc-binding protein 3669071..3669268 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158247 YP_003037702.1 CDS ECBD_3518 NC_012947.1 3669484 3669873 R TIGRFAM: mutator MutT protein; PFAM: NUDIX hydrolase; KEGG: ssn:SSON_0107 nucleoside triphosphate pyrophosphohydrolase, marked preference for dGTP; nucleoside triphosphate pyrophosphohydrolase complement(3669484..3669873) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158248 YP_003037703.1 CDS ECBD_3519 NC_012947.1 3669933 3672638 R functions in protein export; can interact with acidic membrane phospholipids and the SecYEG protein complex; binds to preproteins; binds to ATP and undergoes a conformational change to promote membrane insertion of SecA/bound preprotein; ATP hydrolysis appears to drive release of the preprotein from SecA and deinsertion of SecA from the membrane; additional proteins SecD/F/YajC aid SecA recycling; exists in an equilibrium between monomers and dimers; may possibly form higher order oligomers; proteins in this cluster correspond SecA1; SecA2 is not essential and seems to play a role in secretion of a subset of proteins; preprotein translocase subunit SecA complement(3669933..3672638) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158249 YP_003037704.1 CDS ECBD_3520 NC_012947.1 3672700 3673287 R secM translational pause allows for the initiation of secA translation; SecA regulator SecM complement(3672700..3673287) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158250 YP_003037705.1 CDS lpxC NC_012947.1 3673443 3674360 R zinc-dependent; catalyzes the deacetylation of UDP-(3-O-acyl)-N-acetylglucosamine to UDP-3-O-(3-hydroxytetradecanoyl)-glucosamine in the second step of lipid A biosynthesis; UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase complement(3673443..3674360) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158251 YP_003037706.1 CDS ECBD_3522 NC_012947.1 3674461 3675612 R GTPase; similar structure to tubulin; forms ring-shaped polymers at the site of cell division; other proteins such as FtsA, ZipA, and ZapA, interact with and regulate FtsZ function; cell division protein FtsZ complement(3674461..3675612) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158252 YP_003037707.1 CDS ftsA NC_012947.1 3675673 3676935 R ATP-binding involved in recruitment of FtsK to Z ring; essential cell division protein; colocalizes with FtsZ through direct interaction to the septal ring structure; structurally similar to eukaryotic actin; binds directly to the cell membrane; cell division protein FtsA complement(3675673..3676935) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158253 YP_003037708.1 CDS ECBD_3524 NC_012947.1 3676932 3677762 R involved in septum formation; cell division protein FtsQ complement(3676932..3677762) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158254 YP_003037709.1 CDS ddl NC_012947.1 3677764 3678684 R D-alanine--D-alanine ligase; DdlA; DdlB; cytoplasmic; catalyzes the formation of D-alanyl-D-alanine from two D-alanines in peptidoglycan synthesis; there are two forms of this enzyme in Escherichia coli; D-alanine--D-alanine ligase complement(3677764..3678684) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158255 YP_003037710.1 CDS murC NC_012947.1 3678677 3680152 R Catalyzes the formation of UDP-N-acetylmuramoyl-L-alanine from UDP-N-acetylmuramate and L-alanine in peptidoglycan synthesis; UDP-N-acetylmuramate--L-alanine ligase complement(3678677..3680152) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158256 YP_003037711.1 CDS murG NC_012947.1 3680206 3681273 R UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase; involved in cell wall formation; inner membrane-associated; last step of peptidoglycan synthesis; UDP diphospho-muramoyl pentapeptide beta-N acetylglucosaminyl transferase complement(3680206..3681273) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158257 YP_003037712.1 CDS ECBD_3528 NC_012947.1 3681270 3682514 R integral membrane protein involved in stabilizing FstZ ring during cell division; cell division protein FtsW complement(3681270..3682514) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158258 YP_003037713.1 CDS murD NC_012947.1 3682514 3683830 R UDP-N-acetylmuramoylalanine--D-glutamate ligase; involved in peptidoglycan biosynthesis; cytoplasmic; catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine during cell wall formation; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase complement(3682514..3683830) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158259 YP_003037714.1 CDS mraY NC_012947.1 3683833 3684915 R First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan; phospho-N-acetylmuramoyl-pentapeptide- transferase complement(3683833..3684915) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158260 YP_003037715.1 CDS murF NC_012947.1 3684909 3686267 R TIGRFAM: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate/D-alanyl-D-alanyl ligase; PFAM: Mur ligase middle domain protein; cytoplasmic peptidoglycan synthetase domain protein; KEGG: ssn:SSON_0094 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase complement(3684909..3686267) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158261 YP_003037716.1 CDS murE NC_012947.1 3686264 3687751 R involved in cell wall formation; peptidoglycan synthesis; cytoplasmic enzyme; catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-aceylmuramoyl-l-alanyl-d-glutamate; UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase complement(3686264..3687751) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158262 YP_003037717.1 CDS ECBD_3533 NC_012947.1 3687738 3689504 R PFAM: penicillin-binding protein transpeptidase; Penicillin-binding protein dimerisation domain; KEGG: sdy:SDY_0114 penicillin-binding protein 3; peptidoglycan glycosyltransferase complement(3687738..3689504) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158263 YP_003037718.1 CDS ECBD_3534 NC_012947.1 3689520 3689885 R membrane bound cell division protein at septum containing leucine zipper motif; cell division protein FtsL complement(3689520..3689885) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158264 YP_003037719.1 CDS mraW NC_012947.1 3689882 3690823 R TIGRFAM: S-adenosyl-methyltransferase MraW; PFAM: methyltransferase; KEGG: sbc:SbBS512_E0075 S-adenosyl-methyltransferase MraW; S-adenosyl-methyltransferase MraW complement(3689882..3690823) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158265 YP_003037720.1 CDS ECBD_3536 NC_012947.1 3690825 3691283 R MraZ; UPF0040; crystal structure shows similarity to AbrB; cell division protein MraZ complement(3690825..3691283) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158266 YP_003037721.1 CDS ECBD_3537 NC_012947.1 3691885 3692889 R binds D-fructose as an inducer; involved in regulation of operons for central pathways of carbon metabolism; DNA-binding transcriptional regulator FruR complement(3691885..3692889) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158267 YP_003037722.1 CDS ilvH NC_012947.1 3693069 3693560 R with IlvI catalyzes the formation of 2-acetolactate from pyruvate, the small subunit is required for full activity and valine sensitivity; E.coli produces 3 isoenzymes of acetolactate synthase which differ in specificity to substrates, valine sensitivity and affinity for cofactors; also known as acetolactate synthase 3 small subunit; acetolactate synthase 3 regulatory subunit complement(3693069..3693560) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158268 YP_003037723.1 CDS ECBD_3539 NC_012947.1 3693563 3695287 R catalyzes the formation of 2-acetolactate from pyruvate, leucine sensitive; acetolactate synthase 3 catalytic subunit complement(3693563..3695287) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158269 YP_003037724.1 CDS leuO NC_012947.1 3695605 3696549 R activator for leuABCD operon; member of LysR family of transcriptional activators; leucine transcriptional activator complement(3695605..3696549) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158270 YP_003037725.1 CDS ECBD_3541 NC_012947.1 3697209 3697295 D involved in attenuation control of the leuABCD operon in leucine biosynthesis; leu operon leader peptide 3697209..3697295 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158271 YP_003037726.1 CDS ECBD_3542 NC_012947.1 3697388 3698959 D catalyzes the formation of 2-isopropylmalate from acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis; 2-isopropylmalate synthase 3697388..3698959 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158272 YP_003037727.1 CDS ECBD_3543 NC_012947.1 3698959 3700050 D catalyzes the oxidation of 3-isopropylmalate to 3-carboxy-4-methyl-2-oxopentanoate in leucine biosynthesis; 3-isopropylmalate dehydrogenase 3698959..3700050 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158273 YP_003037728.1 CDS ECBD_3544 NC_012947.1 3700053 3701453 D dehydratase component, catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate; isopropylmalate isomerase large subunit 3700053..3701453 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158274 YP_003037729.1 CDS leuD NC_012947.1 3701464 3702069 D catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate in leucine biosynthesis; forms a heterodimer of LeuC/D; isopropylmalate isomerase small subunit 3701464..3702069 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158275 YP_003037730.1 CDS ECBD_3546 NC_012947.1 3702118 3703296 R TIGRFAM: sugar efflux transporter; PFAM: major facilitator superfamily MFS_1; KEGG: sfx:S0067 transporter; sugar efflux transporter complement(3702118..3703296) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158276 YP_003037731.1 CDS ECBD_3547 NC_012947.1 3703398 3703529 R KEGG: cko:CKO_03311 hypothetical protein; hypothetical protein complement(3703398..3703529) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158277 YP_003037732.1 CDS ECBD_3548 NC_012947.1 3703618 3705273 D activates sgrS under glucose-phosphate stress conditions; transcriptional regulator SgrR 3703618..3705273 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158278 YP_003037733.1 CDS tbpA NC_012947.1 3705437 3706420 D part of the thiamine and TPP transporter tbpA-thiPQ; thiamine transporter substrate binding subunit 3705437..3706420 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158279 YP_003037734.1 CDS thiP NC_012947.1 3706396 3708006 D permease; with TbpA and ThiQ functions in transport of thiamine and thiamine pyrophosphate into the cell; repressed in presence of exogenous thiamine; thiamine transporter membrane protein 3706396..3708006 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158280 YP_003037735.1 CDS thiQ NC_012947.1 3707990 3708688 D with TbpA and ThiP is part of the thiamine and TPP transporter; thiamine transporter ATP-binding subunit 3707990..3708688 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158281 YP_003037736.1 CDS ECBD_3552 NC_012947.1 3708802 3709566 R PFAM: SNARE associated Golgi protein; KEGG: sbo:SBO_0052 hypothetical protein; hypothetical protein complement(3708802..3709566) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158282 YP_003037737.1 CDS ECBD_3553 NC_012947.1 3709652 3710530 R positive and negative regulator; regulates the araBAD and araFGH operons and other genes involved in the transport and catabolism of L-arabinose; AraC family transcriptional regulator complement(3709652..3710530) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158283 YP_003037738.1 CDS ECBD_3554 NC_012947.1 3710869 3712569 D catalyzes the phosphorylation of ribulose to ribulose 5-phosphate; ribulokinase 3710869..3712569 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158284 YP_003037739.1 CDS ECBD_3555 NC_012947.1 3712580 3714082 D catalyzes the formation of L-ribulose from L-arabinose in L-arabinose catabolism; L-arabinose isomerase 3712580..3714082 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158285 YP_003037740.1 CDS araD NC_012947.1 3714367 3715062 D catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate in the anaerobic catabolism of L-ascorbate; links the arabinose metabolic pathway to the pentose phosphate pathway and allows the bacteria to use arabinose as an energy source; L-ribulose-5-phosphate 4-epimerase 3714367..3715062 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158286 YP_003037741.1 CDS ECBD_3557 NC_012947.1 3715137 3717488 D Has polymerase, DNA-binding and 3'-5' exonuclease activities. In Aeropyrum pernix this protein is sensitive to aphidicolin and stable at 95#C; DNA polymerase II 3715137..3717488 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158287 YP_003037742.1 CDS ECBD_3558 NC_012947.1 3717653 3720559 D transcription regulator that activates transcription by stimulating RNA polymerase (RNAP) recycling in case of stress conditions such as supercoiled DNA or high salt concentrations. Probably acts by releasing the RNAP, when it is trapped or immobilized on tightly supercoiled DNA; ATP-dependent helicase HepA 3717653..3720559 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158288 YP_003037743.1 CDS ECBD_3559 NC_012947.1 3720571 3721230 D catalyzes the synthesis of pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32 in the anticodon stem and loop of transfer RNAs; 23S rRNA/tRNA pseudouridine synthase A 3720571..3721230 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158289 YP_003037744.1 CDS djlA NC_012947.1 3721347 3722162 R functions as a co-chaperone with DnaK; involved in regulation of colanic acid capsule; inner membrane protein; dimerized via transmembrane domain; J-like domain is cytoplasmic and can functionally substitute for DnaJ; stimulates synthesis of colanic acid mucoid capsule through the RcsB/C signal transduction system; Dna-J like membrane chaperone protein complement(3721347..3722162) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158290 YP_003037745.1 CDS ECBD_3561 NC_012947.1 3722417 3724771 D determines N-hexane tolerance and is involved in outer membrane permeability; organic solvent tolerance protein 3722417..3724771 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158291 YP_003037746.1 CDS ECBD_3562 NC_012947.1 3724824 3726110 D Chaperone involved in the folding of extracytoplasmic proteins, especially OmpA, OmpF and LamB; peptidyl-prolyl cis-trans isomerase SurA 3724824..3726110 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158292 YP_003037747.1 CDS pdxA NC_012947.1 3726110 3727099 D catalyzes oxidation of 4-(phosphohydroxy)-L-threonine into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which decarboxylates to form 1-amino-3-(phosphohydroxy)propan-2-one (3-amino-2-oxopropyl phosphate); 4-hydroxythreonine-4-phosphate dehydrogenase 3726110..3727099 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158293 YP_003037748.1 CDS ksgA NC_012947.1 3727096 3727917 D catalyzes the transfer of a total of four methyl groups from S-adenosyl-l-methionine (S-AdoMet) to two adjacent adenosine bases A1518 and A1519 in 16S rRNA; mutations in ksgA causes resistance to the translation initiation inhibitor kasugamycin; dimethyladenosine transferase 3727096..3727917 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158294 YP_003037749.1 CDS apaG NC_012947.1 3727920 3728297 D protein associated with Co2+ and Mg2+ efflux; ApaG 3727920..3728297 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158295 YP_003037750.1 CDS apaH NC_012947.1 3728304 3729146 D hydrolyzes P(1),P(4)-bis(5'-adenosyl) tetraphosphate to form 2 ADP; diadenosine tetraphosphatase 3728304..3729146 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158296 YP_003037751.1 CDS folA NC_012947.1 3729224 3729814 R catalyzes the reduction of dihydrofolate to tetrahydrofolate; dihydrofolate reductase complement(3729224..3729814) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158297 YP_003037752.1 CDS ECBD_3568 NC_012947.1 3729895 3731757 R transporter that facilitates potassium-efflux; glutathione-regulated potassium-efflux system protein KefC complement(3729895..3731757) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158298 YP_003037753.1 CDS ECBD_3569 NC_012947.1 3731750 3732280 R Required for full activity of KefC, a potassium-proton antiporter; glutathione-regulated potassium-efflux system ancillary protein KefF complement(3731750..3732280) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158299 YP_003037754.1 CDS ECBD_3570 NC_012947.1 3732388 3733719 R PFAM: major facilitator superfamily MFS_1; general substrate transporter; major facilitator superfamily MFS_1 complement(3732388..3733719) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158300 YP_003037755.1 CDS ECBD_3571 NC_012947.1 3733776 3734063 R KEGG: ssn:SSON_0052 ferredoxin; ferredoxin complement(3733776..3734063) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158301 YP_003037756.1 CDS ECBD_3572 NC_012947.1 3734060 3735346 R FAD/NAD(P)-binding domain; possibly part of an electron transfer system required for anaerobic carnitine reduction; oxidoreductase FixC complement(3734060..3735346) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158302 YP_003037757.1 CDS fixB NC_012947.1 3735397 3736338 R involved in electron transfer during carnitine metabolism; electron transfer flavoprotein FixB complement(3735397..3736338) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158303 YP_003037758.1 CDS ECBD_3574 NC_012947.1 3736353 3737123 R required for anaerobic carnitine reduction, may act to transfer electrons to crotonobetaine reductase; electron transfer flavoprotein FixA complement(3736353..3737123) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158304 YP_003037759.1 CDS ECBD_3575 NC_012947.1 3737597 3739111 D catalyzes the exchange of L-carnitine for gamma-butyrobetaine in carnitine metabolism; L-carnitine/gamma-butyrobetaine antiporter 3737597..3739111 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158305 YP_003037760.1 CDS ECBD_3576 NC_012947.1 3739142 3740284 D catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA; crotonobetainyl-CoA dehydrogenase 3739142..3740284 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158306 YP_003037761.1 CDS caiC NC_012947.1 3741703 3743256 D PFAM: AMP-dependent synthetase and ligase; KEGG: ssn:SSON_0043 crotonobetaine/carnitine-CoA ligase; crotonobetaine/carnitine-CoA ligase 3741703..3743256 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158308 YP_003037762.1 CDS ECBD_3579 NC_012947.1 3743365 3744150 D catalyzes the dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA; carnitinyl-CoA dehydratase 3743365..3744150 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158309 YP_003037763.1 CDS ECBD_3580 NC_012947.1 3744156 3744746 D involved in the synthesis of a cofactor required for carnitine dehydratase and carnitine racemase activities; carnitine operon protein CaiE 3744156..3744746 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158310 YP_003037764.1 CDS ECBD_3581 NC_012947.1 3744955 3745350 R regulator of carnitine metabolism; induces the caiTABCDE and fixABCX operons; CaiF family transcriptional regulator complement(3744955..3745350) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158311 YP_003037765.1 CDS carB NC_012947.1 3745612 3748833 R four CarB-CarA dimers form the carbamoyl phosphate synthetase holoenzyme that catalyzes the production of carbamoyl phosphate; CarB is responsible for the amidotransferase activity; carbamoyl phosphate synthase large subunit complement(3745612..3748833) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158312 YP_003037766.1 CDS ECBD_3583 NC_012947.1 3748851 3749999 R catalyzes production of carbamoyl phosphate from bicarbonate and glutamine in pyrimidine and arginine biosynthesis pathways; forms an octamer composed of four CarAB dimers; carbamoyl phosphate synthase small subunit complement(3748851..3749999) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158313 YP_003037767.1 CDS ECBD_3584 NC_012947.1 3750039 3750176 R KEGG: spq:SPAB_00081 hypothetical protein; hypothetical protein complement(3750039..3750176) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158314 YP_003037768.1 CDS ECBD_3585 NC_012947.1 3750455 3751276 R catalyzes the reduction of 2,3-dihydrodipicolinate to 2,3,4,5-tetrahydrodipicolinate in lysine and diaminopimelate biosynthesis; dihydrodipicolinate reductase complement(3750455..3751276) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158315 YP_003037769.1 CDS ECBD_3586 NC_012947.1 3751443 3752357 R catalyzes the hydrolysis of both purine and pyrimidine ribonucleosides; ribonucleoside hydrolase RihC complement(3751443..3752357) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158316 YP_003037770.1 CDS ispH NC_012947.1 3752423 3753373 R catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP); functions in the nonmevalonate isoprenoid biosynthesis pathway; 4-hydroxy-3-methylbut-2-enyl diphosphate reductase complement(3752423..3753373) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158317 YP_003037771.1 CDS ECBD_3588 NC_012947.1 3753375 3753824 R PFAM: peptidylprolyl isomerase FKBP-type; KEGG: ssn:SSON_0033 probable FKBX-type 16KD peptidyl-prolyl cis-trans isomerase; peptidylprolyl isomerase FKBP-type complement(3753375..3753824) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158318 YP_003037772.1 CDS lspA NC_012947.1 3753949 3754443 R lipoprotein signal peptidase; integral membrane protein that removes signal peptides from prolipoproteins during lipoprotein biosynthesis; lipoprotein signal peptidase complement(3753949..3754443) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158319 YP_003037773.1 CDS ileS NC_012947.1 3754443 3757259 R IleRS; catalyzes the formation of isoleucyl-tRNA(Ile) from isoleucine and tRNA(Ile); since isoleucine and other amino acids such as valine are similar, there are additional editing function in this enzyme; one is involved in hydrolysis of activated valine-AMP and the other is involved in deacylation of mischarged Val-tRNA(Ile); there are two active sites, one for aminoacylation and one for editing; class-I aminoacyl-tRNA synthetase family type 1 subfamily; some organisms carry two different copies of this enzyme; isoleucyl-tRNA synthetase complement(3754443..3757259) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158320 YP_003037774.1 CDS ECBD_3591 NC_012947.1 3757302 3758243 R catalyzes the formation of FMN from riboflavin and the formation of FAD from FMN; in Bacillus the ribC gene has both flavokinase and FAD synthetase activities; bifunctional riboflavin kinase/FMN adenylyltransferase complement(3757302..3758243) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158321 YP_003037775.1 CDS ECBD_3592 NC_012947.1 3758251 3758469 R KEGG: sdy:SDY_0046 hypothetical protein; hypothetical protein complement(3758251..3758469) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158322 YP_003037776.1 CDS rpsT NC_012947.1 3758572 3758835 D binds directly to the 16S rRNA and is involved in post-translational inhibition of arginine and ornithine decarboxylase; 30S ribosomal protein S20 3758572..3758835 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158323 YP_003037777.1 CDS ECBD_3595 NC_012947.1 3759679 3760182 R PFAM: IS1 transposase; KEGG: sty:HCM1.136a IS1 transposase; IS1 transposase complement(3759679..3760182) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156939 YP_003037778.1 CDS ECBD_3596 NC_012947.1 3760101 3760376 R PFAM: Insertion element protein; KEGG: hdu:HD1971 insertion element IS1 1/2/3/5/6 protein InsA; Insertion element protein complement(3760101..3760376) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156940 YP_003037779.1 CDS ECBD_3598 NC_012947.1 3763105 3764064 D KEGG: ssn:SSON_0023 hypothetical protein; hypothetical protein 3763105..3764064 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156942 YP_003037780.1 CDS nhaR NC_012947.1 3764103 3765002 R Na+/H+ antiporter regulatory protein; activates the genes nhaA and osmC; transcriptional activator NhaR complement(3764103..3765002) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156943 YP_003037781.1 CDS nhaA NC_012947.1 3765068 3766234 R exports sodium by using the electrochemical proton gradient to allow protons into the cell; functions in adaptation to high salinity and alkaline pH; activity increases at higher pH; downregulated at acidic pH; pH-dependent sodium/proton antiporter complement(3765068..3766234) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156944 YP_003037782.1 CDS ECBD_3601 NC_012947.1 3766821 3766973 D PFAM: Hok/gef cell toxic protein; KEGG: ssn:SSON_0016 Gef protein interferes with membrane function when in excess; Hok/gef cell toxic protein 3766821..3766973 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156945 YP_003037783.1 CDS ECBD_3602 NC_012947.1 3767004 3767144 R hypothetical protein complement(3767004..3767144) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156946 YP_003037784.1 CDS ECBD_3603 NC_012947.1 3767167 3768279 R PFAM: transposase IS4 family protein; transposase IS4 family protein complement(3767167..3768279) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156947 YP_003037785.1 CDS ECBD_3604 NC_012947.1 3768426 3769556 R chaperone Hsp40; co-chaperone with DnaK; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion; chaperone protein DnaJ complement(3768426..3769556) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156948 YP_003037786.1 CDS dnaK NC_012947.1 3769645 3771561 R heat shock protein 70; assists in folding of nascent polypeptide chains; refolding of misfolded proteins; utilizes ATPase activity to help fold; co-chaperones are DnaJ and GrpE; multiple copies in some bacteria; molecular chaperone DnaK complement(3769645..3771561) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156949 YP_003037787.1 CDS ECBD_3606 NC_012947.1 3771938 3772342 D KEGG: ssn:SSON_0013 hypothetical protein; hypothetical protein 3771938..3772342 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156950 YP_003037788.1 CDS ECBD_3607 NC_012947.1 3772368 3773081 D PFAM: protein of unknown function UPF0174; KEGG: ssn:SSON_0012 hypothetical protein; hypothetical protein 3772368..3773081 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156951 YP_003037789.1 CDS ECBD_3608 NC_012947.1 3773230 3773796 D PFAM: GPR1/FUN34/yaaH family protein; KEGG: ssn:SSON_0011 hypothetical protein; hypothetical protein 3773230..3773796 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156952 YP_003037790.1 CDS mogA NC_012947.1 3773831 3774418 R forms a trimer; related to eukaryotic protein gephyrin; functions during molybdenum cofactor biosynthesis; molybdenum cofactor biosynthesis protein MogA complement(3773831..3774418) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156953 YP_003037791.1 CDS ECBD_3610 NC_012947.1 3774533 3775486 R KEGG: sbc:SbBS512_E0008 transaldolase; TIGRFAM: transaldolase; PFAM: transaldolase; transaldolase B complement(3774533..3775486) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156954 YP_003037792.1 CDS ECBD_3611 NC_012947.1 3775765 3777195 D TIGRFAM: amino acid carrier protein; PFAM: sodium:alanine symporter; KEGG: elf:LF82_2462 uncharacterized transporter YaaJ; amino acid carrier protein 3775765..3777195 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156955 YP_003037793.1 CDS ECBD_3612 NC_012947.1 3777265 3778041 D PFAM: protein of unknown function DUF328; KEGG: sdy:SDY_0006 hypothetical protein; hypothetical protein 3777265..3778041 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156956 YP_003037794.1 CDS ECBD_3613 NC_012947.1 3778194 3778490 R KEGG: ssn:SSON_0005 hypothetical protein; hypothetical protein complement(3778194..3778490) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156957 YP_003037795.1 CDS ECBD_3614 NC_012947.1 3778703 3779989 R catalyzes the formation of L-threonine from O-phospho-L-homoserine; threonine synthase complement(3778703..3779989) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156958 YP_003037796.1 CDS ECBD_3615 NC_012947.1 3779990 3780922 R catalyzes the formation of O-phospho-L-homoserine from L-homoserine in threonine biosynthesis from asparate; homoserine kinase complement(3779990..3780922) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156959 YP_003037797.1 CDS thrA NC_012947.1 3780924 3783386 R multifunctional homotetrameric enzyme that catalyzes the phosphorylation of aspartate to form aspartyl-4-phosphate as well as conversion of aspartate semialdehyde to homoserine; functions in a number of amino acid biosynthetic pathways; bifunctional aspartokinase I/homoserine dehydrogenase I complement(3780924..3783386) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156960 YP_003037798.1 CDS ECBD_3617 NC_012947.1 3783746 3784432 R member of the SPOUT superfamily of methyltransferases; RNA methyltransferase complement(3783746..3784432) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156961 YP_003037799.1 CDS ECBD_3618 NC_012947.1 3784832 3784972 R KEGG: sfx:S4705 hypothetical protein; hypothetical protein complement(3784832..3784972) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156962 YP_003037800.1 CDS ECBD_3619 NC_012947.1 3785068 3785784 D PFAM: response regulator receiver; transcriptional regulator domain protein; SMART: response regulator receiver; KEGG: sfv:SFV_4435 two-component response regulator; two-component response regulator 3785068..3785784 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156963 YP_003037801.1 CDS ECBD_3620 NC_012947.1 3785844 3787196 R PFAM: inner membrane protein CreD; KEGG: sbc:SbBS512_E4948 inner membrane protein CreD; hypothetical protein complement(3785844..3787196) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156964 YP_003037802.1 CDS ECBD_3621 NC_012947.1 3787254 3788678 R part of a two-component regulatory system with CreB or PhoB; involved in catabolic regulation; sensory histidine kinase CreC complement(3787254..3788678) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156965 YP_003037803.1 CDS ECBD_3622 NC_012947.1 3788678 3789367 R response regulator in two-component regulatory system with CreC; CreB protein is phosphorylated by sensor protein phospho-CreC; involved in catabolic regulation; DNA-binding response regulator CreB complement(3788678..3789367) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156966 YP_003037804.1 CDS ECBD_3623 NC_012947.1 3789380 3789853 R PFAM: CreA family protein; KEGG: sfv:SFV_4431 hypothetical protein; hypothetical protein complement(3789380..3789853) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156967 YP_003037805.1 CDS ECBD_3624 NC_012947.1 3790064 3790933 D PFAM: transcription activator effector binding; helix-turn-helix- domain containing protein AraC type; SMART: helix-turn-helix- domain containing protein AraC type; KEGG: sfv:SFV_4430 right origin-binding protein; AraC family transcriptional regulator 3790064..3790933 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156968 YP_003037806.1 CDS ECBD_3625 NC_012947.1 3790930 3791577 R catalyzes reactions involving the transfer of phospho groups between the three carbon atoms of phosphoglycerate; phosphoglycerate mutase complement(3790930..3791577) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156969 YP_003037807.1 CDS ECBD_3626 NC_012947.1 3791629 3792150 D pyrophosphatase; has activity against dUTP and dITP; the crystal structure of the Vibrio protein showed similarity to Methanococcus janaschii Mj0226; in Vibrio cholerae this gene is part of the Mba operon that is involved in regulation and maintenance of biofilms; in Escherichia coli overexpression of this gene leads to resistance to an HMP analog; NTPase 3791629..3792150 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156970 YP_003037808.1 CDS ECBD_3627 NC_012947.1 3792235 3792561 R When complexed with L-tryptophan it binds the operator region of the trp operon and prevents the initiation of transcription; Trp operon repressor complement(3792235..3792561) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156971 YP_003037809.1 CDS ECBD_3628 NC_012947.1 3792651 3794588 R catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine residues in peptidoglycan; lytic murein transglycosylase complement(3792651..3794588) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156972 YP_003037810.1 CDS ECBD_3629 NC_012947.1 3794799 3796466 D ChvD; in Agrobacterium tumefaciens, mutations in both Walker boxes were found to affect virulence; ABC transporter ATP-binding protein 3794799..3796466 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156973 YP_003037811.1 CDS ECBD_3630 NC_012947.1 3796773 3798005 R catalyzes the formation of NAD(+) from nicotinamide ribonucleotide; nicotinamide-nucleotide adenylyltransferase complement(3796773..3798005) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156974 YP_003037812.1 CDS ECBD_3631 NC_012947.1 3798026 3799408 R Sms; stabilizes the strand-invasion intermediate during the DNA repair; involved in recombination of donor DNA and plays an important role in DNA damage repair after exposure to mutagenic agents; DNA repair protein RadA complement(3798026..3799408) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156975 YP_003037813.1 CDS serB NC_012947.1 3799457 3800425 R catalyzes the formation of serine from O-phosphoserine; phosphoserine phosphatase complement(3799457..3800425) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156976 YP_003037814.1 CDS ECBD_3633 NC_012947.1 3800531 3801175 D KEGG: sbo:SBO_4450 hypothetical protein; hypothetical protein 3800531..3801175 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156977 YP_003037815.1 CDS lplA NC_012947.1 3801203 3802219 D Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl on the lipoate-dependent enzymes. Creates an amide linkage that joins the free carboxyl group of lipoic acid to the epsilon-amino group of a specific lysine residue in lipoyl domain of apoproteins; lipoate-protein ligase A 3801203..3802219 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156978 YP_003037816.1 CDS ECBD_3635 NC_012947.1 3802251 3802514 D PFAM: helix-turn-helix domain protein; SMART: helix-turn-helix domain protein; KEGG: sfv:SFV_4419 hypothetical protein; helix-turn-helix domain protein 3802251..3802514 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156979 YP_003037817.1 CDS deoD NC_012947.1 3802675 3803394 R catalyzes the reversible phosphorolysis of ribonucleosides and 2'- deoxyribonucleosides to the free base and (2'-deoxy)ribose-1- phosphate; purine nucleoside phosphorylase complement(3802675..3803394) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156980 YP_003037818.1 CDS ECBD_3637 NC_012947.1 3803474 3804697 R catalyzes the transfer of phosphate between the C1 and C5 carbons of pentose; phosphopentomutase complement(3803474..3804697) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156981 YP_003037819.1 CDS deoA NC_012947.1 3804749 3806071 R Catalyzes the reversible phosphorolysis of thymidine, deoxyuridine and their analogues to their respective bases and 2-deoxyribose 1-phosphate; thymidine phosphorylase complement(3804749..3806071) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156982 YP_003037820.1 CDS ECBD_3639 NC_012947.1 3806198 3806977 R catalyzes the formation of D-glyceraldehyde 3-phosphate and acetaldehyde from 2-deoxy-D-ribose-5-phosphate; deoxyribose-phosphate aldolase complement(3806198..3806977) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156983 YP_003037821.1 CDS ECBD_3640 NC_012947.1 3807235 3808785 D hypothetical protein 3807235..3808785 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156984 YP_003037822.1 CDS ECBD_3641 NC_012947.1 3808757 3809620 D PFAM: radical SAM protein; 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: sfv:SFV_4413 activating enzyme; radical SAM protein 3808757..3809620 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156985 YP_003037823.1 CDS ECBD_3642 NC_012947.1 3809838 3810617 R PFAM: TatD-related deoxyribonuclease; KEGG: elf:LF82_3504 uncharacterized deoxyribonuclease YjjV; deoxyribonuclease YjjV complement(3809838..3810617) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156986 YP_003037824.1 CDS ECBD_3643 NC_012947.1 3810614 3811687 R PFAM: patatin; KEGG: ssn:SSON_4528 hypothetical protein; patatin complement(3810614..3811687) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156987 YP_003037825.1 CDS ECBD_3644 NC_012947.1 3811809 3811970 R PFAM: protein of unknown function DUF1328; KEGG: sbc:SbBS512_E4920 hypothetical protein; hypothetical protein complement(3811809..3811970) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156988 YP_003037826.1 CDS ECBD_3645 NC_012947.1 3812097 3812696 R PFAM: transport-associated; SMART: transport-associated and nodulation region; KEGG: ssn:SSON_4526 periplasmic protein; periplasmic protein complement(3812097..3812696) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156989 YP_003037827.1 CDS prfC NC_012947.1 3813095 3814684 R stimulates the release of release factors 1 and 2 from the ribosome after hydrolysis of the ester bond in peptidyl-tRNA has occurred; GDP/GTP-binding protein; peptide chain release factor 3 complement(3813095..3814684) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156990 YP_003037828.1 CDS ECBD_3647 NC_012947.1 3814775 3815452 R manganese-dependent 5'-nucleotidase; specific for 5'-UMP, 5'-dUMP, and 5'-dTMP; member of haloacid dehalogenase (HAD)-like hydrolase superfamily; nucleotidase complement(3814775..3815452) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156991 YP_003037829.1 CDS rimI NC_012947.1 3815467 3815913 R alanine acetyltransferase that specifically acetylates ribosomal protein S18; ribosomal-protein-alanine N-acetyltransferase complement(3815467..3815913) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156992 YP_003037830.1 CDS ECBD_3649 NC_012947.1 3815882 3816295 R with the chi subunit binds to single-strand binding (SSB) protein and acts as a bridge between the DnaX clamp loader complex and the SSB; DNA polymerase III subunit psi complement(3815882..3816295) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156993 YP_003037831.1 CDS rsmC NC_012947.1 3816398 3817429 D 16S rRNA M2G1207 methyltransferase; one of many enzymes that modify bases of the ribosomal RNA; this enzyme methylates the G at position 1207 of the small ribosomal subunit; 16S ribosomal RNA m2G1207 methyltransferase 3816398..3817429 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156994 YP_003037832.1 CDS ECBD_3651 NC_012947.1 3818058 3818294 R PFAM: protein of unknown function DUF1435; KEGG: sfv:SFV_4401 inner membrane protein; hypothetical protein complement(3818058..3818294) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156998 YP_003037833.1 CDS ECBD_3652 NC_012947.1 3818435 3819223 D PFAM: ferric iron reductase; KEGG: sbo:SBO_4427 ferric iron reductase involved in ferric hydroximate transport; ferric iron reductase involved in ferric hydroximate transport 3818435..3819223 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156999 YP_003037834.1 CDS ECBD_3653 NC_012947.1 3819261 3819884 R regulator for the transport and utilization of the aromatic beta-glucosides arbutin and silicin; BglJ family transcriptional regulator complement(3819261..3819884) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157000 YP_003037835.1 CDS ECBD_3654 NC_012947.1 3819896 3820621 R PFAM: regulatory protein LuxR; SMART: regulatory protein LuxR; LuxR family transcriptional regulator complement(3819896..3820621) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157001 YP_003037836.1 CDS ECBD_3655 NC_012947.1 3821240 3822010 D PFAM: protein of unknown function DUF1212; KEGG: sfv:SFV_4395 structural protein; hypothetical protein 3821240..3822010 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157002 YP_003037837.1 CDS ECBD_3656 NC_012947.1 3822001 3822474 D KEGG: sfv:SFV_4394 hypothetical protein; hypothetical protein 3822001..3822474 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157003 YP_003037838.1 CDS ECBD_3657 NC_012947.1 3822581 3823120 D This protein is required for primosome-dependent normal DNA replication; it is also involved in inducing stable DNA replication during SOS response. It forms, in concert with dnaB protein and other prepriming proteins dnaC, N, N', N'' a prepriming protein complex on the specific site of the template DNA recognized by protein N'; primosomal protein DnaI 3822581..3823120 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157004 YP_003037839.1 CDS ECBD_3658 NC_012947.1 3823123 3823860 D acts to load the DnaB helicase onto the initiation site during DNA replication; DNA replication protein DnaC 3823123..3823860 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157005 YP_003037840.1 CDS ECBD_3659 NC_012947.1 3823909 3824403 D KEGG: sbc:SbBS512_E4900 hypothetical protein; hypothetical protein 3823909..3824403 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157006 YP_003037841.1 CDS ECBD_3660 NC_012947.1 3824657 3826948 D catalyzes the transfer of phosphoglycerol to the glucan backbone; phosphoglycerol transferase I 3824657..3826948 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157007 YP_003037842.1 CDS ECBD_3661 NC_012947.1 3827086 3828108 R PFAM: alcohol dehydrogenase GroES domain protein; alcohol dehydrogenase zinc-binding domain protein; KEGG: ssn:SSON_4504 oxidoreductase; alcohol dehydrogenase GroES domain protein complement(3827086..3828108) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157008 YP_003037843.1 CDS ECBD_3663 NC_012947.1 3829375 3830736 D PFAM: major facilitator superfamily MFS_1; major facilitator superfamily MFS_1 3829375..3830736 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157010 YP_003037844.1 CDS ECBD_3664 NC_012947.1 3830785 3832440 R PFAM: chemotaxis sensory transducer; ligand binding Tar domain protein; histidine kinase HAMP region domain protein; SMART: chemotaxis sensory transducer; histidine kinase HAMP region domain protein; ligand binding Tar domain protein; KEGG: sbo:SBO_4414 methyl-accepting chemotaxis protein I; methyl-accepting chemotaxis sensory transducer complement(3830785..3832440) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157011 YP_003037845.1 CDS ECBD_3665 NC_012947.1 3832559 3833005 R KEGG: sfv:SFV_4385 regulator of the 4HPA-hydroxylase operon; TIGRFAM: homoprotocatechuate degradation operon regulator, HpaR; PFAM: regulatory protein MarR; SMART: regulatory protein MarR; MarR family transcriptional regulator complement(3832559..3833005) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157012 YP_003037846.1 CDS ECBD_3666 NC_012947.1 3833277 3834566 D KEGG: sbo:SBO_4412 5-oxo-1,2,5-tricarboxilic-3-penten acid decarboxilase/isomerase; TIGRFAM: 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase, HpaG1 subunit; 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase,HpaG2 subunit; PFAM: fumarylacetoacetate hydrolase; 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase, HpaG1 subunit 3833277..3834566 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157013 YP_003037847.1 CDS ECBD_3667 NC_012947.1 3834563 3836029 D TIGRFAM: 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase; PFAM: aldehyde dehydrogenase; KEGG: sbc:SbBS512_E4889 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase; 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase 3834563..3836029 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157014 YP_003037848.1 CDS ECBD_3668 NC_012947.1 3836031 3836882 D KEGG: sbc:SbBS512_E4888 3,4-dihydroxyphenylacetate 2,3-dioxygenase; TIGRFAM: 3,4-dihydroxyphenylacetate 2,3-dioxygenase; PFAM: extradiol ring-cleavage dioxygenase class III protein subunit B; 3,4-dihydroxyphenylacetate 2,3-dioxygenase 3836031..3836882 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157015 YP_003037849.1 CDS ECBD_3669 NC_012947.1 3836892 3837272 D PFAM: 5-carboxymethyl-2-hydroxymuconate isomerase; KEGG: sfx:S4651 5-carboxymethyl-2-hydroxymuconate delta-isomerase; 5-carboxymethyl-2-hydroxymuconate delta-isomerase 3836892..3837272 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157016 YP_003037850.1 CDS ECBD_3670 NC_012947.1 3837340 3838143 D KEGG: ssn:SSON_4495 2-oxo-hept-3-ene-1,7-dioate hydratase; TIGRFAM: 2-oxo-hepta-3-ene-1,7-dioic acid hydratase; PFAM: fumarylacetoacetate hydrolase; 2-oxo-hepta-3-ene-1,7-dioic acid hydratase 3837340..3838143 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157017 YP_003037851.1 CDS ECBD_3671 NC_012947.1 3838154 3838942 D KEGG: ssn:SSON_4494 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (HHED aldolase); TIGRFAM: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; PFAM: HpcH/HpaI aldolase; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase 3838154..3838942 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157018 YP_003037852.1 CDS ECBD_3672 NC_012947.1 3839066 3840442 D TIGRFAM: 4-hydroxyphenylacetate transporter; PFAM: major facilitator superfamily MFS_1; KEGG: sbo:SBO_4406 4-hydroxyphenylacetate permease; 4-hydroxyphenylacetate transporter 3839066..3840442 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158242 YP_003037853.1 CDS ECBD_3673 NC_012947.1 3840452 3841342 D KEGG: efe:EFER_3069 regulator of the 4HPA-hydroxylase operon; TIGRFAM: 4-hydroxyphenylacetate catabolism regulatory protein HpaA; PFAM: helix-turn-helix- domain containing protein AraC type; Cupin 2 conserved barrel domain protein; SMART: helix-turn-helix- domain containing protein AraC type; AraC family transcriptional regulator 3840452..3841342 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158494 YP_003037854.1 CDS ECBD_3674 NC_012947.1 3841593 3843155 D TIGRFAM: 4-hydroxyphenylacetate 3-monooxygenase, oxygenase subunit; PFAM: 4-hydroxyphenylacetate 3-hydroxylase; KEGG: efe:EFER_3070 4-hydroxyphenylacetate 3-monooxygenase large component; 4-hydroxyphenylacetate 3-monooxygenase, oxygenase subunit 3841593..3843155 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158581 YP_003037855.1 CDS ECBD_3675 NC_012947.1 3843173 3843685 D TIGRFAM: 4-hydroxyphenylacetate 3-monooxygenase, reductase subunit; PFAM: flavin reductase domain protein FMN-binding; KEGG: sbc:SbBS512_E4881 4-hydroxyphenylacetate 3-monooxygenase, reductase component; 4-hydroxyphenylacetate 3-monooxygenase, reductase subunit 3843173..3843685 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158582 YP_003037856.1 CDS ECBD_3676 NC_012947.1 3844078 3846228 D PFAM: carbon starvation protein CstA; KEGG: sbc:SbBS512_E4880 carbon starvation family protein; carbon starvation protein CstA 3844078..3846228 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158583 YP_003037857.1 CDS ECBD_3677 NC_012947.1 3846278 3846481 D PFAM: protein of unknown function DUF466; KEGG: sfv:SFV_4372 hypothetical protein; hypothetical protein 3846278..3846481 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158584 YP_003037858.1 CDS ECBD_3678 NC_012947.1 3846492 3847448 D PFAM: cobalamin synthesis protein P47K; cobalamin synthesis CobW domain protein; KEGG: efe:EFER_3075 GTPase; GTP-binding protein YjiA 3846492..3847448 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158585 YP_003037859.1 CDS ECBD_3679 NC_012947.1 3847494 3848408 R PFAM: restriction endonuclease; KEGG: ypb:YPTS_4095 restriction endonuclease; restriction endonuclease complement(3847494..3848408) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158586 YP_003037860.1 CDS hsdR NC_012947.1 3848596 3852108 D restriction endonuclease; the EcoKI enzyme recognizes 5'-AACN(6)GTGC-3'; subunit R is required for both nuclease and ATPase activities; type I restriction enzyme EcoKI subunit R 3848596..3852108 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158587 YP_003037861.1 CDS ECBD_3681 NC_012947.1 3852309 3853898 D PFAM: N-6 DNA methylase; KEGG: efe:EFER_3080 DNA methylase M; Site-specific DNA-methyltransferase (adenine-specific) 3852309..3853898 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158588 YP_003037862.1 CDS ECBD_3683 NC_012947.1 3854670 3854945 D PFAM: Insertion element protein; KEGG: hdu:HD1971 insertion element IS1 1/2/3/5/6 protein InsA; Insertion element protein 3854670..3854945 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158590 YP_003037863.1 CDS ECBD_3684 NC_012947.1 3854864 3855367 D PFAM: IS1 transposase; KEGG: sty:HCM1.136a IS1 transposase; IS1 transposase 3854864..3855367 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158591 YP_003037864.1 CDS ECBD_3685 NC_012947.1 3856323 3856664 D involved in the degradation of RNA; may be involved in the recycling of RNA during the SOS response; endoribonuclease SymE 3856323..3856664 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158592 YP_003037865.1 CDS ECBD_3686 NC_012947.1 3856808 3858205 D McrB protein together with McrC forms the McrBC restriction system; recognizes N4- and C5-methylcytosine (and 5-hydroxy-methylcytosines); appears to act against 5-methylcytosine preceded by a purine residue; 5-methylcytosine-specific restriction enzyme subunit McrB 3856808..3858205 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158593 YP_003037866.1 CDS ECBD_3687 NC_012947.1 3858205 3859251 D McrC protein together with McrB forms the McrBC restriction system; recognizes N4- and C5-methylcytosine (and 5-hydroxy-methylcytosines); appears to act against 5-methylcytosine preceded by a purine residue; MrcC modulates the specificty of McrB and has DNA cleavage activity; 5-methylcytosine-specific restriction enzyme subunit McrC 3858205..3859251 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158594 YP_003037867.1 CDS ECBD_3689 NC_012947.1 3862677 3863180 R PFAM: IS1 transposase; KEGG: dps:DP0860 insertion element IS1 1/5/6 protein (InsB); IS1 transposase complement(3862677..3863180) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158596 YP_003037868.1 CDS ECBD_3690 NC_012947.1 3863099 3863374 R PFAM: Insertion element protein; KEGG: hdu:HD1971 insertion element IS1 1/2/3/5/6 protein InsA; Insertion element protein complement(3863099..3863374) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158597 YP_003037869.1 CDS ECBD_3691 NC_012947.1 3863429 3864196 R PFAM: transposase YhgA family protein; KEGG: efe:EFER_3089 conserved hypothetical protein, transposase; transposase YhgA family protein complement(3863429..3864196) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158598 YP_003037870.1 CDS ECBD_3692 NC_012947.1 3864233 3864406 R hypothetical protein complement(3864233..3864406) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158599 YP_003037871.1 CDS ECBD_3693 NC_012947.1 3864675 3865907 D PFAM: major facilitator superfamily MFS_1; KEGG: sbc:SbBS512_E4767 multidrug resistance protein MdtM; major facilitator superfamily MFS_1 3864675..3865907 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158600 YP_003037872.1 CDS ECBD_3694 NC_012947.1 3865948 3867228 D PFAM: protein of unknown function DUF445; KEGG: sbc:SbBS512_E4768 putatitve membrane protein; hypothetical protein 3865948..3867228 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158601 YP_003037873.1 CDS ECBD_3695 NC_012947.1 3867344 3868495 D PFAM: 2-hydroxyglutaryl-CoA dehydratase D-component; KEGG: elf:LF82_3487 uncharacterized protein YjiM; 2-hydroxyglutaryl-CoA dehydratase D-component 3867344..3868495 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158602 YP_003037874.1 CDS ECBD_3696 NC_012947.1 3868505 3869272 D KEGG: sbc:SbBS512_E4770 (R)-2-hydroxyglutaryl-CoA dehydratase activator; TIGRFAM: CoA-substrate-specific enzyme activase; PFAM: ATPase BadF/BadG/BcrA/BcrD type; CoA-substrate-specific enzyme activase 3868505..3869272 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158603 YP_003037875.1 CDS ECBD_3697 NC_012947.1 3869269 3869526 D KEGG: sbo:SBO_4385 hypothetical protein; hypothetical protein 3869269..3869526 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158604 YP_003037876.1 CDS ECBD_3698 NC_012947.1 3869480 3870451 D PFAM: SdiA-regulated domain protein; KEGG: sbo:SBO_4384 hypothetical protein; SdiA-regulated domain protein 3869480..3870451 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158605 YP_003037877.1 CDS ECBD_3699 NC_012947.1 3870519 3871697 D PFAM: protein of unknown function DUF1228; major facilitator superfamily MFS_1; KEGG: elf:LF82_3484 uncharacterized protein YjiJ; hypothetical protein 3870519..3871697 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158606 YP_003037878.1 CDS ECBD_3700 NC_012947.1 3871710 3872264 R in yeast cells this enzyme removes the 2'-phosphate from RNA via an intermediate in which the phosphate is ADP-ribosylated by NAD followed by a presumed transesterification to release the RNA and generate ADP-ribose 1''-2''-cyclic phosphate; functions in tRNA splicing; the function and substrate of bacterial enzymes in organisms that do not perform splicing is unknown; RNA 2'-phosphotransferase-like protein complement(3871710..3872264) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158607 YP_003037879.1 CDS ECBD_3701 NC_012947.1 3872514 3873197 D PFAM: nucleoside recognition domain protein; KEGG: sbc:SbBS512_E4775 transporter; nucleoside recognition domain protein 3872514..3873197 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158608 YP_003037880.1 CDS ECBD_3702 NC_012947.1 3873194 3873655 D PFAM: nucleoside recognition domain protein; KEGG: sbo:SBO_4380 hypothetical protein; hypothetical protein 3873194..3873655 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158609 YP_003037881.1 CDS ECBD_3703 NC_012947.1 3873668 3874840 D TIGRFAM: isoaspartyl dipeptidase; PFAM: amidohydrolase; amidohydrolase; KEGG: sbc:SbBS512_E4777 beta-aspartyl peptidase; isoaspartyl dipeptidase 3873668..3874840 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158610 YP_003037882.1 CDS ECBD_3704 NC_012947.1 3874905 3875816 D PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: elf:LF82_3481 uncharacterized HTH-type transcriptional regulator; DNA-binding transcriptional regulator 3874905..3875816 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158611 YP_003037883.1 CDS iraD NC_012947.1 3875809 3876201 R inhibitor of RssB activity in response to DNA damage; blocks degradation of stationary phase sigma factor RpoS; DNA replication/recombination/repair protein complement(3875809..3876201) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158612 YP_003037884.1 CDS ECBD_3706 NC_012947.1 3876167 3876301 R hypothetical protein complement(3876167..3876301) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158613 YP_003037885.1 CDS ECBD_3707 NC_012947.1 3876397 3876540 R KEGG: sbc:SbBS512_E4782 hypothetical protein; hypothetical protein complement(3876397..3876540) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158614 YP_003037886.1 CDS ECBD_3708 NC_012947.1 3876519 3876620 R hypothetical protein complement(3876519..3876620) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158615 YP_003037887.1 CDS ECBD_3709 NC_012947.1 3876874 3877704 D KEGG: ssn:SSON_4480 hypothetical protein; hypothetical protein 3876874..3877704 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158616 YP_003037888.1 CDS ECBD_3710 NC_012947.1 3877845 3878618 R regulates the expression of uxuBA; DNA-binding transcriptional repressor UxuR complement(3877845..3878618) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158617 YP_003037889.1 CDS ECBD_3711 NC_012947.1 3878617 3878811 D KEGG: sbc:SbBS512_E4790 hypothetical protein; hypothetical protein 3878617..3878811 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158618 YP_003037890.1 CDS ECBD_3712 NC_012947.1 3878833 3880293 R PFAM: Mannitol dehydrogenase domain; Mannitol dehydrogenase rossman domain; KEGG: ssn:SSON_4478 D-mannonate oxidoreductase; mannitol dehydrogenase complement(3878833..3880293) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158619 YP_003037891.1 CDS ECBD_3713 NC_012947.1 3880374 3881558 R catalyzes the formation of 2-dehydro-3-deoxy-D-gluconate from mannonate; mannonate dehydratase complement(3880374..3881558) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158620 YP_003037892.1 CDS ECBD_3714 NC_012947.1 3881898 3883241 D TIGRFAM: gluconate transporter; PFAM: gluconate transporter; citrate transporter; fructuronate transporter 3881898..3883241 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158621 YP_003037893.1 CDS ECBD_3715 NC_012947.1 3883484 3884386 R PFAM: FimH mannose-binding domain protein; fimbrial protein; KEGG: sfv:SFV_4206 FimH protein precursor; FimH mannose-binding domain protein complement(3883484..3884386) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158622 YP_003037894.1 CDS ECBD_3716 NC_012947.1 3884406 3884909 R PFAM: fimbrial protein; KEGG: sfv:SFV_4207 minor fimbrial subunit, precursor polypeptide; fimbrial protein complement(3884406..3884909) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158623 YP_003037895.1 CDS ECBD_3717 NC_012947.1 3884922 3885452 R PFAM: fimbrial protein; fimbrial protein complement(3884922..3885452) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158624 YP_003037896.1 CDS ECBD_3718 NC_012947.1 3885462 3888098 R PFAM: fimbrial biogenesis outer membrane usher protein; fimbrial biogenesis outer membrane usher protein complement(3885462..3888098) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158625 YP_003037897.1 CDS ECBD_3719 NC_012947.1 3888165 3888890 R PFAM: Pili assembly chaperone, N-terminal; Pili assembly chaperone, C-terminal; KEGG: sfx:S4463 periplasmic chaperone; Pili assembly chaperone, N-terminal complement(3888165..3888890) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158626 YP_003037898.1 CDS ECBD_3720 NC_012947.1 3888927 3889466 R PFAM: fimbrial protein; KEGG: sfx:S4464 fimbrial protein; fimbrial protein complement(3888927..3889466) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158627 YP_003037899.1 CDS ECBD_3721 NC_012947.1 3889531 3890079 R PFAM: fimbrial protein; KEGG: sbo:SBO_4364 major type 1 subunit fimbrin; fimbrial protein complement(3889531..3890079) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158628 YP_003037900.1 CDS ECBD_3722 NC_012947.1 3890561 3891157 R inversion of on/off regulator of fimA; tyrosine recombinase complement(3890561..3891157) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158629 YP_003037901.1 CDS ECBD_3723 NC_012947.1 3891825 3892100 D PFAM: Insertion element protein; KEGG: hdu:HD1971 insertion element IS1 1/2/3/5/6 protein InsA; Insertion element protein 3891825..3892100 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158630 YP_003037902.1 CDS ECBD_3724 NC_012947.1 3892019 3892522 D PFAM: IS1 transposase; KEGG: dps:DP0860 insertion element IS1 1/5/6 protein (InsB); IS1 transposase 3892019..3892522 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158631 YP_003037903.1 CDS ECBD_3725 NC_012947.1 3892516 3893013 R PFAM: integrase; integrase complement(3892516..3893013) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158632 YP_003037904.1 CDS ECBD_3726 NC_012947.1 3894469 3895185 D PFAM: oligogalacturonate-specific porin; KEGG: sfv:SFV_4216 hypothetical protein; oligogalacturonate-specific porin 3894469..3895185 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158633 YP_003037905.1 CDS ECBD_3727 NC_012947.1 3895205 3896311 D PFAM: Kelch repeat-containing protein; Kelch repeat protein; KEGG: sfv:SFV_4217 hypothetical protein; N-acetylneuraminic acid mutarotase 3895205..3896311 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158634 YP_003037906.1 CDS ECBD_3728 NC_012947.1 3896376 3897356 D PFAM: protein of unknown function DUF303 acetylesterase KEGG: sfv:SFV_4218 hypothetical protein; hypothetical protein 3896376..3897356 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158635 YP_003037907.1 CDS ECBD_3730 NC_012947.1 3899917 3900174 D KEGG: kpn:KPN_00259 hypothetical protein; hypothetical protein 3899917..3900174 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158637 YP_003037908.1 CDS ECBD_3731 NC_012947.1 3900186 3900731 D PFAM: GCN5-related N-acetyltransferase; KEGG: tgr:Tgr7_0152 GCN5-related N-acetyltransferase; GCN5-related N-acetyltransferase 3900186..3900731 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158638 YP_003037909.1 CDS ECBD_3732 NC_012947.1 3900787 3901533 D PFAM: methyltransferase type 11; methyltransferase type 12; KEGG: kpe:KPK_4969 methyltransferase family protein; methyltransferase type 11 3900787..3901533 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158639 YP_003037910.1 CDS ECBD_3733 NC_012947.1 3902319 3903440 D PFAM: peptidase M42 family protein; KEGG: stm:STM1612 cellulase protein; peptidase M42 family protein 3902319..3903440 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158640 YP_003037911.1 CDS ECBD_3734 NC_012947.1 3903437 3903715 D PFAM: phosphotransferase system lactose/cellobiose-specific IIB subunit; KEGG: sea:SeAg_B1548 PTS system, lactose/cellobiose specific IIB subunit; protein-N(pi)-phosphohistidine--sugar phosphotransferase 3903437..3903715 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158641 YP_003037912.1 CDS ECBD_3735 NC_012947.1 3903727 3905040 D TIGRFAM: PTS system, galactitol-specific IIC subunit; PFAM: PTS system Galactitol-specific IIC component; KEGG: sea:SeAg_B1547 PTS system, IIc component; PTS system, galactitol-specific IIC subunit 3903727..3905040 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158642 YP_003037913.1 CDS ECBD_3736 NC_012947.1 3905053 3905859 D PFAM: photosystem I assembly BtpA; KEGG: stm:STM1615 nucleoside triphosphatase; photosystem I assembly BtpA 3905053..3905859 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158643 YP_003037914.1 CDS ECBD_3737 NC_012947.1 3905990 3906421 D PFAM: phosphoenolpyruvate-dependent sugar phosphotransferase system EIIA 2; KEGG: sea:SeAg_B1545 phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2; PTS IIA-like nitrogen-regulatory protein PtsN 3905990..3906421 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158644 YP_003037915.1 CDS ECBD_3738 NC_012947.1 3906433 3907065 D PFAM: ribulose-phosphate 3-epimerase; KEGG: sew:SeSA_A1734 protein SgcE; epimerase 3906433..3907065 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158645 YP_003037916.1 CDS ECBD_3739 NC_012947.1 3907082 3907864 D PFAM: regulatory protein DeoR; SMART: regulatory protein DeoR; KEGG: sea:SeAg_B1543 regulatory protein; DeoR family transcriptional regulator 3907082..3907864 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158646 YP_003037917.1 CDS ECBD_3740 NC_012947.1 3908054 3908329 D PFAM: Insertion element protein; KEGG: dps:DP0861 insertion element iso-IS1d protein (InsA); Insertion element protein 3908054..3908329 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158647 YP_003037918.1 CDS ECBD_3741 NC_012947.1 3908248 3908751 D PFAM: IS1 transposase; KEGG: dps:DP0860 insertion element IS1 1/5/6 protein (InsB); IS1 transposase 3908248..3908751 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158648 YP_003037919.1 CDS ECBD_3742 NC_012947.1 3908834 3909091 R hypothetical protein complement(3908834..3909091) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158649 YP_003037920.1 CDS ECBD_3745 NC_012947.1 3910841 3911362 D Member of the extracytoplasmic function sigma factors which are active under specific conditions; binds with the catalytic core of RNA polymerase to produce the holoenzyme and directs bacterial core RNA polymerase to specific promoter elements to initiate transcription; this sigma factor regulates the genes for iron dicitrate transport; RNA polymerase sigma factor FecI 3910841..3911362 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158652 YP_003037921.1 CDS ECBD_3746 NC_012947.1 3911359 3912312 D with FecA forms the FecAR signal transduction system; ferric citrate binds FecA and transmits a signal across the outer membrane to FecR. FecR transmits a signal across the cytoplasmic membrane and activates the sigma 70 protein FecI that directs the RNA polymerase to express the fecABCDE operon; fec operon regulator FecR 3911359..3912312 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158653 YP_003037922.1 CDS ECBD_3747 NC_012947.1 3912399 3914723 D TIGRFAM: TonB-dependent siderophore receptor; PFAM: TonB-dependent receptor; TonB-dependent receptor plug; Secretin/TonB short domain; KEGG: sdy:SDY_4297 citrate-dependent iron transport, outer membrane receptor; TonB-dependent siderophore receptor 3912399..3914723 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158654 YP_003037923.1 CDS fecB NC_012947.1 3914768 3915670 D part of the ABC transporter involved in the uptake of citrate-dependent Fe(3+); iron-dicitrate transporter substrate-binding subunit 3914768..3915670 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158655 YP_003037924.1 CDS fecC NC_012947.1 3915667 3916665 D part of the FecBCDE citrate-dependent iron (III) transporter; iron-dicitrate transporter permease subunit 3915667..3916665 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158656 YP_003037925.1 CDS fecD NC_012947.1 3916662 3917618 D ferric citrate binds FecA and is transported across the outer membrane while transmits a signal across the cytoplasmic membrane protein FecR. FecR transmits a signal across the membrane and activates the cytoplasmic FecI that directs the RNA polymerase to express the fecABCDE operon (which encodes the ferric citrate outer membrane receptor and the ferric citrate ABC transporter), as well as fecIR. FecD is one of two (along with FecC) integral membrane protein components of the iron dicitrate ABC transporter.; iron-dicitrate transporter subunit FecD 3916662..3917618 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158657 YP_003037926.1 CDS fecE NC_012947.1 3917619 3918386 D Part of the FecBCDE citrate-dependent iron (III) transporter; iron-dicitrate transporter ATP-binding subunit 3917619..3918386 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158658 YP_003037927.1 CDS ECBD_3752 NC_012947.1 3918943 3919356 R KEGG: eca:ECA2935 hypothetical protein; hypothetical protein complement(3918943..3919356) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158659 YP_003037928.1 CDS ECBD_3754 NC_012947.1 3920134 3920436 D PFAM: transposase IS3/IS911 family protein; KEGG: sfl:SF1439 IS600 ORF1; transposase IS3/IS911 family protein 3920134..3920436 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158661 YP_003037929.1 CDS ECBD_3755 NC_012947.1 3920472 3921290 D PFAM: Integrase catalytic region; KEGG: sfl:SF1901 IS600 ORF2; Integrase catalytic region 3920472..3921290 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158576 YP_003037930.1 CDS ECBD_3756 NC_012947.1 3921444 3923441 D TIGRFAM: choline/carnitine/betaine transporter; PFAM: BCCT transporter; KEGG: sbc:SbBS512_E0764 transporter BCCT family; choline/carnitine/betaine transporter 3921444..3923441 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160130 YP_003037931.1 CDS ECBD_3757 NC_012947.1 3923504 3923917 D KEGG: kpn:KPN_pKPN3p05959 hypothetical protein; hypothetical protein 3923504..3923917 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160131 YP_003037932.1 CDS ECBD_3759 NC_012947.1 3924747 3925019 R PFAM: transposase IS3/IS911 family protein; KEGG: sbc:SbBS512_E2307 IS911, transposase orfA; transposase IS3/IS911 family protein complement(3924747..3925019) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160133 YP_003037933.1 CDS ECBD_3760 NC_012947.1 3925240 3925590 D KEGG: ent:Ent638_1538 lipoprotein; hypothetical protein 3925240..3925590 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160134 YP_003037934.1 CDS ECBD_3761 NC_012947.1 3925643 3925768 R hypothetical protein complement(3925643..3925768) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160135 YP_003037935.1 CDS ECBD_3762 NC_012947.1 3925811 3926929 R PFAM: oxidoreductase domain protein; semialdehyde dehydrogenase NAD - binding; oxidoreductase domain; KEGG: pmu:PM1759 hypothetical protein; oxidoreductase domain protein complement(3925811..3926929) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160136 YP_003037936.1 CDS ECBD_3763 NC_012947.1 3926941 3928158 R PFAM: general substrate transporter; major facilitator superfamily MFS_1; KEGG: ses:SARI_01868 sialic acid transporter; sialic acid transporter complement(3926941..3928158) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160137 YP_003037937.1 CDS ECBD_3764 NC_012947.1 3928785 3930113 D PFAM: transposase IS4 family protein; transposase IS4 family protein 3928785..3930113 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160138 YP_003037938.1 CDS ECBD_3766 NC_012947.1 3932940 3934130 R PFAM: integrase; KEGG: ent:Ent638_0463 phage integrase; integrase complement(3932940..3934130) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160140 YP_003037939.1 CDS ECBD_3767 NC_012947.1 3934671 3935690 D PFAM: alcohol dehydrogenase GroES domain protein; alcohol dehydrogenase zinc-binding domain protein; KEGG: sfv:SFV_4229 oxidoreductase; alcohol dehydrogenase GroES domain protein 3934671..3935690 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160142 YP_003037940.1 CDS idnK NC_012947.1 3935694 3936257 R KEGG: elf:LF82_1090 thermosensitive gluconokinase; TIGRFAM: carbohydrate kinase, thermoresistant glucokinase family; PFAM: shikimate kinase; D-gluconate kinase complement(3935694..3936257) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160143 YP_003037941.1 CDS ECBD_3769 NC_012947.1 3936474 3937505 D NAD-binding; L-idonate 5-dehydrogenase 3936474..3937505 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160144 YP_003037942.1 CDS ECBD_3770 NC_012947.1 3937529 3938293 D Involved in the nonphosphorylative, ketogenic oxidation of glucose and oxidizes gluconate to 5-ketogluconate; gluconate 5-dehydrogenase 3937529..3938293 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160145 YP_003037943.1 CDS ECBD_3771 NC_012947.1 3938356 3939675 D TIGRFAM: gluconate transporter; PFAM: gluconate transporter; citrate transporter; TRAP C4-dicarboxylate transporter permease DctM subunit; KEGG: ssn:SSON_4450 L-idonate transporter; gluconate transporter 3938356..3939675 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160146 YP_003037944.1 CDS ECBD_3772 NC_012947.1 3939742 3940740 D PFAM: regulatory protein LacI; periplasmic binding protein/LacI transcriptional regulator; SMART: regulatory protein LacI; LacI family transcriptional regulator 3939742..3940740 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160147 YP_003037945.1 CDS ECBD_3773 NC_012947.1 3940818 3942320 D PFAM: protein of unknown function DUF853 NPT hydrolase KEGG: ssn:SSON_4448 hypothetical protein; hypothetical protein 3940818..3942320 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160148 YP_003037946.1 CDS ECBD_3774 NC_012947.1 3942481 3943563 R PFAM: permease YjgP/YjgQ family protein; KEGG: elf:LF82_3475 inner membrane protein YjgQ; permease YjgP/YjgQ family protein complement(3942481..3943563) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160149 YP_003037947.1 CDS ECBD_3775 NC_012947.1 3943563 3944663 R PFAM: permease YjgP/YjgQ family protein; KEGG: sbo:SBO_4179 hypothetical protein; permease YjgP/YjgQ family protein complement(3943563..3944663) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160150 YP_003037948.1 CDS ECBD_3776 NC_012947.1 3944930 3946441 D catalyzes the removal of N-terminal amino acids preferably leucine from various peptides; leucyl aminopeptidase 3944930..3946441 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160151 YP_003037949.1 CDS ECBD_3777 NC_012947.1 3946795 3947238 D binds to single-strand binding (SSB) protein and acts as a bridge between the DnaX clamp loader complex and the SSB; DNA polymerase III subunit chi 3946795..3947238 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160152 YP_003037950.1 CDS valS NC_012947.1 3947238 3950093 D valine--tRNA ligase; ValRS; converts valine ATP and tRNA(Val) to AMP PPi and valyl-tRNA(Val); class-I aminoacyl-tRNA synthetase type 1 subfamily; has a posttransfer editing process to hydrolyze mischarged Thr-tRNA(Val) which is done by the editing domain; valyl-tRNA synthetase 3947238..3950093 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160153 YP_003037951.1 CDS ECBD_3779 NC_012947.1 3950149 3951345 R PFAM: protein of unknown function DUF898 transmembrane; KEGG: sfx:S4493 hypothetical protein; hypothetical protein complement(3950149..3951345) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160154 YP_003037952.1 CDS ECBD_3780 NC_012947.1 3951538 3952041 D PFAM: GCN5-related N-acetyltransferase; GCN5-related N-acetyltransferase 3951538..3952041 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160155 YP_003037953.1 CDS ECBD_3781 NC_012947.1 3952087 3952503 R PFAM: protein of unknown function DUF1260; KEGG: sfl:SF4234 hypothetical protein; hypothetical protein complement(3952087..3952503) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160156 YP_003037954.1 CDS ECBD_3782 NC_012947.1 3952665 3953669 D catalyzes the formation of L-citrulline from carbamoyl phosphate and L-ornithine in arginine biosynthesis and degradation; E. coli K12 has two genes, argF and argI, capable of producing functional ornithine carbamoyltransferase. When both are active in the same cell, the two gene products form a family of four hybrid trimeric isoenzymes designated, FFF, FFI, FII, and III.; ornithine carbamoyltransferase subunit I 3952665..3953669 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160157 YP_003037955.1 CDS ECBD_3783 NC_012947.1 3953742 3955394 R KEGG: sbo:SBO_4194 hypothetical protein; hypothetical protein complement(3953742..3955394) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160158 YP_003037956.1 CDS ECBD_3784 NC_012947.1 3955517 3955969 R PFAM: conserved hypothetical protein; KEGG: sdy:SDY_4270 hypothetical protein; hypothetical protein complement(3955517..3955969) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160159 YP_003037957.1 CDS ECBD_3785 NC_012947.1 3956114 3956707 R PFAM: regulatory protein TetR; KEGG: sbc:SbBS512_E4834 TetR family transcriptional regulator; TetR family transcriptional regulator complement(3956114..3956707) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160160 YP_003037958.1 CDS ECBD_3786 NC_012947.1 3956778 3957491 D PFAM: short-chain dehydrogenase/reductase SDR; KEGG: ssn:SSON_4429 oxidoreductase; oxidoreductase 3956778..3957491 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160161 YP_003037959.1 CDS ECBD_3787 NC_012947.1 3957622 3958017 D PFAM: endoribonuclease L-PSP; KEGG: ssn:SSON_4428 hypothetical protein; endoribonuclease L-PSP 3957622..3958017 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160162 YP_003037960.1 CDS pyrB NC_012947.1 3958436 3959371 D catalyzes the transfer of the carbamoyl moiety from carbamoyl phosphate to L- aspartate in pyrimidine biosynthesis; aspartate carbamoyltransferase catalytic subunit 3958436..3959371 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160163 YP_003037961.1 CDS ECBD_3789 NC_012947.1 3959384 3959845 D involved in the allosteric regulation of aspartate carbamoyltransferase; aspartate carbamoyltransferase regulatory subunit 3959384..3959845 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160164 YP_003037962.1 CDS ECBD_3790 NC_012947.1 3959918 3960304 D TIGRFAM: endoribonuclease L-PSP; PFAM: endoribonuclease L-PSP; KEGG: sdy:SDY_4262 hypothetical protein; endoribonuclease L-PSP 3959918..3960304 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160165 YP_003037963.1 CDS ECBD_3791 NC_012947.1 3960510 3963206 R P-type ATPase involved in magnesium influx; magnesium-transporting ATPase MgtA complement(3960510..3963206) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160166 YP_003037964.1 CDS treR NC_012947.1 3963585 3964532 D regulates genes involved in trehalose metabolism; binds the intermediate trehalose-6-phosphate; binds a dimer; regulates treBC operon; trehalose repressor 3963585..3964532 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160167 YP_003037965.1 CDS ECBD_3793 NC_012947.1 3964651 3966072 D phosphoenolpyruvate-dependent sugar phosphotransferase system; catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane; IIB is phosphorylated by IIA and then transfers the phosphoryl group to the sugar; IIC forms the translocation channel; PTS system trehalose(maltose)-specific transporter subunits IIBC 3964651..3966072 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160168 YP_003037966.1 CDS ECBD_3794 NC_012947.1 3966122 3967777 D KEGG: sfx:S4513 trehalose-6-phosphate hydrolase; TIGRFAM: alpha,alpha-phosphotrehalase; PFAM: alpha amylase catalytic region; SMART: alpha amylase catalytic sub domain; trehalose-6-phosphate hydrolase 3966122..3967777 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160169 YP_003037967.1 CDS ECBD_3795 NC_012947.1 3967749 3967925 D hypothetical protein 3967749..3967925 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160170 YP_003037968.1 CDS ECBD_3796 NC_012947.1 3968171 3970309 D Catalyzes the reduction of nucleoside 5'-triphosphates to 2'-deoxynucleoside 5'-triphosphates; anaerobic ribonucleoside triphosphate reductase 3968171..3970309 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160171 YP_003037969.1 CDS nrdG NC_012947.1 3970468 3970932 D activates anaerobic ribonucleoside-triphosphate reductase under anaerobic conditions; anaerobic ribonucleotide reductase-activating protein 3970468..3970932 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160172 YP_003037970.1 CDS ECBD_3798 NC_012947.1 3970977 3971363 R PFAM: cytochrome b562; KEGG: sfv:SFV_4255 cytochrome b(562); cytochrome b562 complement(3970977..3971363) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160173 YP_003037971.1 CDS ECBD_3799 NC_012947.1 3971546 3972898 R protease involved in proteolytic processing of the antibiotic Microcin B17 and in sensitivity to the DNA gyrase inhibitor LetD; peptidase PmbA complement(3971546..3972898) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160174 YP_003037972.1 CDS ECBD_3800 NC_012947.1 3972993 3973544 D PFAM: protein of unknown function DUF615; KEGG: ssn:SSON_4415 hypothetical protein; hypothetical protein 3972993..3973544 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160175 YP_003037973.1 CDS ECBD_3801 NC_012947.1 3973700 3975073 R TIGRFAM: UDP-N-acetylmuramate; PFAM: cytoplasmic peptidoglycan synthetase domain protein; Mur ligase middle domain protein; KEGG: elf:LF82_1383 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso- diaminop; UDP-N-acetylmuramate complement(3973700..3975073) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160176 YP_003037974.1 CDS ECBD_3802 NC_012947.1 3975249 3976247 D catalyzes the formation of D-fructose 6-phosphate from fructose-1,6-bisphosphate; fructose-1,6-bisphosphatase 3975249..3976247 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160177 YP_003037975.1 CDS ECBD_3803 NC_012947.1 3976280 3977275 R membrane component of a sugar ABC transporter system; inner membrane ABC transporter permease YjfF complement(3976280..3977275) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160178 YP_003037976.1 CDS ECBD_3804 NC_012947.1 3977262 3978284 R PFAM: inner-membrane translocator; KEGG: elf:LF82_3716 inner membrane ABC transporter permease YtfT; inner-membrane translocator complement(3977262..3978284) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160179 YP_003037977.1 CDS ECBD_3805 NC_012947.1 3978298 3979800 R PFAM: ABC transporter; SMART: ATPase AAA; KEGG: elf:LF82_3715 uncharacterized ABC transporter ATP-binding protein YtfR; ABC transporter complement(3978298..3979800) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160180 YP_003037978.1 CDS ECBD_3806 NC_012947.1 3979940 3980896 R PFAM: periplasmic binding protein/LacI transcriptional regulator; KEGG: sbc:SbBS512_E4856 sugar ABC transporter periplasmic sugar-binding protein; periplasmic binding protein/LacI transcriptional regulator complement(3979940..3980896) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160181 YP_003037979.1 CDS ECBD_3807 NC_012947.1 3981206 3981736 D catalyzes the hydrolysis of pyrophosphate to phosphate; inorganic pyrophosphatase 3981206..3981736 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160182 YP_003037980.1 CDS ECBD_3808 NC_012947.1 3982116 3982457 R PFAM: AIG2 family protein; KEGG: sfv:SFV_4266 hypothetical protein; AIG2 family protein complement(3982116..3982457) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160183 YP_003037981.1 CDS ECBD_3809 NC_012947.1 3982460 3986239 R PFAM: protein of unknown function DUF490; KEGG: elf:LF82_3712 uncharacterized protein YtfN; hypothetical protein complement(3982460..3986239) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160184 YP_003037982.1 CDS ECBD_3810 NC_012947.1 3986236 3987969 R PFAM: surface antigen (D15); surface antigen variable number repeat protein; KEGG: sbc:SbBS512_E4861 outer membrane protein, OMP85 family; surface antigen (D15) complement(3986236..3987969) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160185 YP_003037983.1 CDS ECBD_3811 NC_012947.1 3988175 3988813 D this stereospecific enzymes reduces the S isomer of methionine sulfoxide while MsrB reduces the R form; provides protection against oxidative stress; methionine sulfoxide reductase A 3988175..3988813 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160186 YP_003037984.1 CDS ECBD_3812 NC_012947.1 3989136 3990479 D PFAM: protein of unknown function DUF21; CBS domain containing protein; transporter-associated region; KEGG: elf:LF82_3710 UPF0053 inner membrane protein YtfL; hypothetical protein 3989136..3990479 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160187 YP_003037985.1 CDS ECBD_3813 NC_012947.1 3990541 3990747 R PFAM: protein of unknown function DUF1107; KEGG: efe:EFER_4276 hypothetical protein; hypothetical protein complement(3990541..3990747) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160188 YP_003037986.1 CDS ECBD_3814 NC_012947.1 3991072 3991629 D KEGG: elf:LF82_3708 uncharacterized protein YtfJ; hypothetical protein 3991072..3991629 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160189 YP_003037987.1 CDS ECBD_3815 NC_012947.1 3991619 3992359 R catalyzes the formation of AMP from adenosine-3',5'-bisphosphate; adenosine-3'(2'),5'-bisphosphate nucleotidase complement(3991619..3992359) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160190 YP_003037988.1 CDS cpdB NC_012947.1 3992549 3994492 D periplasmic enzyme; functions during ribonucleic acid degradation; 2',3'-cyclic nucleotides are first converted to 3'-nucleotide and then cleaved to yield a ribonucleotide and a phosphate; bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein 3992549..3994492 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160191 YP_003037989.1 CDS ECBD_3817 NC_012947.1 3994615 3994995 R PFAM: helix-turn-helix HxlR type; KEGG: sfv:SFV_4276 hypothetical protein; HxlR family transcriptional regulator complement(3994615..3994995) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160192 YP_003037990.1 CDS ECBD_3818 NC_012947.1 3995084 3995944 D PFAM: NmrA family protein; 3-beta hydroxysteroid dehydrogenase/isomerase; KEGG: sbo:SBO_4231 oxidoreductase; NmrA family protein 3995084..3995944 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160193 YP_003037991.1 CDS ECBD_3819 NC_012947.1 3996053 3997018 D PFAM: protein of unknown function DUF6 transmembrane; KEGG: ssn:SSON_4395 transmembrane subunit; hypothetical protein 3996053..3997018 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160194 YP_003037992.1 CDS ECBD_3820 NC_012947.1 3997126 3997788 D Involved in anaerobic NO protection and iron metabolism; iron-sulfur cluster repair di-iron protein 3997126..3997788 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160195 YP_003037993.1 CDS ECBD_3821 NC_012947.1 3997833 3999245 R involved in the transport across the cytoplasmic membrane of D-alanine, D-serine and glycine; D-alanine/D-serine/glycine permease complement(3997833..3999245) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160196 YP_003037994.1 CDS ECBD_3822 NC_012947.1 3999554 4000174 R FKBP-type; rotamase; catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides; peptidyl-prolyl cis-trans isomerase complement(3999554..4000174) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160197 YP_003037995.1 CDS ECBD_3823 NC_012947.1 4000393 4001031 D PFAM: Opacity-associated protein A; Opacity-associated protein A domain protein; KEGG: sdy:SDY_4376 hypothetical protein; Opacity-associated protein A 4000393..4001031 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160198 YP_003037996.1 CDS ECBD_3824 NC_012947.1 4001015 4001662 R PFAM: regulatory protein TetR; KEGG: sbc:SbBS512_E4747 TetR family transcriptional regulator; TetR family transcriptional regulator complement(4001015..4001662) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160199 YP_003037997.1 CDS ECBD_3825 NC_012947.1 4001875 4002666 D catalyzes the first of the two reduction steps in the elongation cycle of fatty acid synthesis; 3-ketoacyl-ACP reductase 4001875..4002666 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160200 YP_003037998.1 CDS ECBD_3826 NC_012947.1 4002676 4003518 D PFAM: xylose isomerase; KEGG: sbo:SBO_4242 hypothetical protein; xylose isomerase 4002676..4003518 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160201 YP_003037999.1 CDS ECBD_3827 NC_012947.1 4003528 4004304 D PFAM: enoyl-CoA hydratase/isomerase; KEGG: sbo:SBO_4243 hypothetical protein; enoyl-CoA hydratase/isomerase 4003528..4004304 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160202 YP_003038000.1 CDS ECBD_3828 NC_012947.1 4004314 4005855 D PFAM: coenzyme A transferase; KEGG: sbc:SbBS512_E4742 CoA-transferase; coenzyme A transferase 4004314..4005855 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160203 YP_003038001.1 CDS ECBD_3829 NC_012947.1 4005852 4007924 D PFAM: fumarate reductase/succinate dehydrogenase flavoprotein domain protein; FAD dependent oxidoreductase; KEGG: elf:LF82_p707 oxidoreductase; fumarate reductase/succinate dehydrogenase flavoprotein domain protein 4005852..4007924 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160204 YP_003038002.1 CDS ECBD_3830 NC_012947.1 4008017 4009294 D PFAM: major facilitator superfamily MFS_1; KEGG: elf:LF82_p706 hexuronate transporter; major facilitator superfamily MFS_1 4008017..4009294 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160205 YP_003038003.1 CDS rplI NC_012947.1 4009396 4009845 R in Escherichia coli this protein is wrapped around the base of the L1 stalk; 50S ribosomal protein L9 complement(4009396..4009845) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160206 YP_003038004.1 CDS rpsR NC_012947.1 4009887 4010114 R binds as a heterodimer with protein S6 to the central domain of the 16S rRNA; helps stabilize the platform of the 30S subunit; 30S ribosomal protein S18 complement(4009887..4010114) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160207 YP_003038005.1 CDS ECBD_3833 NC_012947.1 4010119 4010433 R binds single-stranded DNA at the primosome assembly site; primosomal replication protein N complement(4010119..4010433) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160208 YP_003038006.1 CDS rpsF NC_012947.1 4010440 4010835 R binds cooperatively with S18 to the S15-16S complex, allowing platform assembly to continue with S11 and S21; 30S ribosomal protein S6 complement(4010440..4010835) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158577 YP_003038007.1 CDS ECBD_3835 NC_012947.1 4011163 4011438 D PFAM: protein of unknown function DUF1471; KEGG: sfv:SFV_4355 hypothetical protein; hypothetical protein 4011163..4011438 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158578 YP_003038008.1 CDS sgaE NC_012947.1 4011567 4012253 R catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate in the anaerobic catabolism of L-ascorbate; links the arabinose metabolic pathway to the pentose phosphate pathway and allows the bacteria to use arabinose as an energy source; L-ribulose-5-phosphate 4-epimerase complement(4011567..4012253) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158406 YP_003038009.1 CDS ECBD_3837 NC_012947.1 4012253 4013107 R UlaE; catalyzes the epimerization of L-ribulose-5-phosphate into L-xylulose-5-phosphate; part of the anaerobic L-ascorbate degradation pathway; L-xylulose 5-phosphate 3-epimerase complement(4012253..4013107) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158407 YP_003038010.1 CDS ulaD NC_012947.1 4013117 4013767 R catalyzes the formation of L-xylulose-5-phosphate from 3-keto-L-gulonate-6-phosphate in anaerobic L-ascorbate utilization; 3-keto-L-gulonate-6-phosphate decarboxylase complement(4013117..4013767) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158408 YP_003038011.1 CDS ECBD_3839 NC_012947.1 4013781 4014245 R involved in the phosphorylation and transport of sugars across the cell membrane; protein IIA transfers a phosphoryl group to IIB which then transfers the phosphoryl group to the sugar; IIC forms the translocation channel for the sugar uptake; PTS system L-ascorbate-specific transporter subunit IIA complement(4013781..4014245) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158409 YP_003038012.1 CDS ECBD_3840 NC_012947.1 4014255 4014560 R involved in the phosphorylation and transport of sugars across the cell membrane; protein IIA transfers a phosphoryl group to IIB which then transfers the phosphoryl group to the sugar; IIC forms the translocation channel for the sugar uptake; PTS system L-ascorbate-specific transporter subunit IIB complement(4014255..4014560) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158410 YP_003038013.1 CDS ulaA NC_012947.1 4014576 4015973 R membrane component; functions with enzymes IIB (sgaB; ulaB) and IIA (sgaA; ulaC) enzyme I and HPr for anaerobic utilization and uptake of L-ascorbate; sgaTBA are regulated by yifQ as well as Crp and Fnr; PTS system ascorbate-specific transporter subunit IIC complement(4014576..4015973) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158411 YP_003038014.1 CDS ECBD_3842 NC_012947.1 4016328 4017392 D KEGG: ssn:SSON_4374 L-ascorbate 6-phosphate lactonase; L-ascorbate 6-phosphate lactonase 4016328..4017392 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158412 YP_003038015.1 CDS ECBD_3843 NC_012947.1 4017500 4018255 D negative regulator of ulaG and ulaABCDEF; transcriptional repressor UlaR 4017500..4018255 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158413 YP_003038016.1 CDS ECBD_3844 NC_012947.1 4018252 4019001 R YjfP; esterase activity towards palmitoyl-CoA and pNP-butyrate in vitro; unknown function and substrate in vivo; esterase complement(4018252..4019001) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158414 YP_003038017.1 CDS ECBD_3845 NC_012947.1 4019183 4019512 D in Escherichia coli, mutation of this gene affects biofilm maturation, stress response, and motility; biofilm stress and motility protein A 4019183..4019512 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158415 YP_003038018.1 CDS ECBD_3846 NC_012947.1 4019661 4019936 D PFAM: protein of unknown function DUF1471; KEGG: sfv:SFV_4344 hypothetical protein; hypothetical protein 4019661..4019936 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158416 YP_003038019.1 CDS ECBD_3847 NC_012947.1 4020053 4021678 R catalyzes the formation of 3-methylcrotonyl-CoA from isovaleryl-CoA; isovaleryl CoA dehydrogenase complement(4020053..4021678) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158417 YP_003038020.1 CDS ECBD_3848 NC_012947.1 4021762 4022925 R PFAM: glutathionylspermidine synthase; KEGG: sfx:S4608 synthetase/amidase; glutathionylspermidine synthase complement(4021762..4022925) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158418 YP_003038021.1 CDS ECBD_3849 NC_012947.1 4022928 4023566 R PFAM: protein of unknown function DUF1190; KEGG: sfv:SFV_4341 hypothetical protein; hypothetical protein complement(4022928..4023566) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158419 YP_003038022.1 CDS ECBD_3850 NC_012947.1 4023576 4023974 R PFAM: protein of unknown function DUF350; hypothetical protein complement(4023576..4023974) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158420 YP_003038023.1 CDS ECBD_3851 NC_012947.1 4023992 4024651 R KEGG: sbo:SBO_4272 hypothetical protein; hypothetical protein complement(4023992..4024651) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158421 YP_003038024.1 CDS ECBD_3852 NC_012947.1 4024702 4025400 R PFAM: PspA/IM30 family protein; phage shock protein A, PspA complement(4024702..4025400) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158422 YP_003038025.1 CDS ECBD_3853 NC_012947.1 4025419 4025820 R hypothetical protein complement(4025419..4025820) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158423 YP_003038026.1 CDS ECBD_3854 NC_012947.1 4025947 4026678 R Specifically methylates the ribose of guanosine 2251 in 23S rRNA; 23S rRNA (guanosine-2'-O-)-methyltransferase complement(4025947..4026678) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158424 YP_003038027.1 CDS ECBD_3855 NC_012947.1 4026858 4029299 R 3'-5'exoribonuclease that acts nonspecifically on poly(A), poly(U) and ribosomal RNAs; exoribonuclease R complement(4026858..4029299) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158425 YP_003038028.1 CDS ECBD_3856 NC_012947.1 4029338 4029763 R negatively regulates the transcription of genes upregulated by nitrosative stress; transcriptional repressor NsrR complement(4029338..4029763) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158426 YP_003038029.1 CDS ECBD_3857 NC_012947.1 4029968 4031266 R catalyzes the formation of N6-(1,2,-dicarboxyethyl)-AMP from L-aspartate, inosine monophosphate and GTP in AMP biosynthesis; adenylosuccinate synthetase complement(4029968..4031266) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158427 YP_003038030.1 CDS ECBD_3858 NC_012947.1 4031370 4031567 R KEGG: sfv:SFV_4334 hypothetical protein; hypothetical protein complement(4031370..4031567) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158428 YP_003038031.1 CDS ECBD_3859 NC_012947.1 4031649 4032653 R with HflK inhibits proteolysis of lambda cII protein by FtsH; FtsH protease regulator HflC complement(4031649..4032653) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158429 YP_003038032.1 CDS ECBD_3860 NC_012947.1 4032656 4033915 R with HflC inhibits proteolysis of lambda cII protein by FtsH; FtsH protease regulator HflK complement(4032656..4033915) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158430 YP_003038033.1 CDS ECBD_3861 NC_012947.1 4034001 4035281 R involved in modulation of proteins HflK and HflC; part of the hflA locus (high frequency of lysogenization) which governs the lysis-lysogeny decision of bacteriophage lambda by controlling stability of the phage cII protein; GTPase HflX complement(4034001..4035281) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158431 YP_003038034.1 CDS hfq NC_012947.1 4035357 4035665 R Stimulates the elongation of poly(A) tails; RNA-binding protein Hfq complement(4035357..4035665) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158432 YP_003038035.1 CDS miaA NC_012947.1 4035751 4036701 R IPP transferase; isopentenyltransferase; involved in tRNA modification; in Escherichia coli this enzyme catalyzes the addition of a delta2-isopentenyl group from dimethylallyl diphosphate to the N6-nitrogen of adenosine adjacent to the anticodon of tRNA species that read codons starting with uracil; further tRNA modifications may occur; mutations in miaA result in defects in translation efficiency and fidelity; tRNA delta(2)-isopentenylpyrophosphate transferase complement(4035751..4036701) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158433 YP_003038036.1 CDS mutL NC_012947.1 4036694 4038541 R This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. Promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex; DNA mismatch repair protein complement(4036694..4038541) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158434 YP_003038037.1 CDS ECBD_3865 NC_012947.1 4038551 4039888 R KEGG: ssn:SSON_4354 N-acetylmuramoyl-L-alanine amidase II; PFAM: cell wall hydrolase/autolysin; SMART: cell wall hydrolase/autolysin; N-acetylmuramoyl-l-alanine amidase II complement(4038551..4039888) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158435 YP_003038038.1 CDS ECBD_3866 NC_012947.1 4039907 4040368 R possibly involved in cell wall synthesis; ATPase complement(4039907..4040368) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158436 YP_003038039.1 CDS ECBD_3867 NC_012947.1 4040340 4041887 R TIGRFAM: carbohydrate kinase, YjeF related protein; PFAM: protein of unknown function UPF0031; YjeF-family domain protein; KEGG: ssn:SSON_4351 hypothetical protein; hypothetical protein complement(4040340..4041887) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158437 YP_003038040.1 CDS ECBD_3868 NC_012947.1 4041886 4043025 D TIGRFAM: iron-sulfur cluster binding protein; PFAM: domain of unknown function DUF1730; KEGG: sbc:SbBS512_E4697 iron-sulfur cluster binding protein; iron-sulfur cluster binding protein 4041886..4043025 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158438 YP_003038041.1 CDS ECBD_3869 NC_012947.1 4043804 4044349 R 3'-5' exoribonuclease specific for small oligoribonuclotides; oligoribonuclease complement(4043804..4044349) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158442 YP_003038042.1 CDS ECBD_3870 NC_012947.1 4044444 4045496 D EngC; RsgA; CpgA; circularly permuted GTPase; ribosome small subunit-dependent GTPase A; has the pattern G4-G1-G3 as opposed to other GTPases; interacts strongly with 30S ribosome which stimulates GTPase activity; ribosome-associated GTPase 4044444..4045496 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158443 YP_003038043.1 CDS psd NC_012947.1 4045593 4046561 D catalyzes the decarboxylation of phosphatidyl-L-serine to phosphatidylethanoleamine; phosphatidylserine decarboxylase 4045593..4046561 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158444 YP_003038044.1 CDS ECBD_3872 NC_012947.1 4046583 4049906 D PFAM: MscS Mechanosensitive ion channel; KEGG: sbo:SBO_4298 predicted mechanosensitive channel; hypothetical protein 4046583..4049906 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158445 YP_003038045.1 CDS ECBD_3873 NC_012947.1 4050056 4051558 R PFAM: amino acid permease-associated region; KEGG: elf:LF82_3454 inner membrane transporter YjeM; amino acid permease-associated region complement(4050056..4051558) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158446 YP_003038046.1 CDS ECBD_3874 NC_012947.1 4051777 4052754 R lysine--tRNA ligase beta chain; poxA; class II aminoacyl tRNA synthetase; catalyzes a two-step reaction; first charging a lysine molecule by linking the carboxyl group to the alpha-phosphate of ATP; second by transfer of the aminoacyl-adenylate to its tRNA; lysyl-tRNA synthetase complement(4051777..4052754) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158447 YP_003038047.1 CDS ECBD_3875 NC_012947.1 4053079 4054887 D part of four member fumarate reductase enzyme complex FrdABCD which catalyzes the reduction of fumarate to succinate during anaerobic respiration; FrdAB are the catalytic subcomplex consisting of a flavoprotein subunit and an iron-sulfur subunit, respectively; FrdCD are the membrane components which interact with quinone and are involved in electron transfer; the catalytic subunits are similar to succinate dehydrogenase SdhAB; fumarate reductase flavoprotein subunit 4053079..4054887 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158448 YP_003038048.1 CDS ECBD_3876 NC_012947.1 4054880 4055614 D part of four member fumarate reductase enzyme complex FrdABCD which catalyzes the reduction of fumarate to succinate during anaerobic respiration; FrdAB are the catalytic subcomplex consisting of a flavoprotein subunit and an iron-sulfur subunit, respectively; FrdCD are the membrane components which interact with quinone and are involved in electron transfer; the catalytic subunits are similar to succinate dehydrogenase SdhAB; fumarate reductase iron-sulfur subunit 4054880..4055614 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158449 YP_003038049.1 CDS ECBD_3877 NC_012947.1 4055625 4056020 D part of four member fumarate reductase enzyme complex FrdABCD which catalyzes the reduction of fumarate to succinate during anaerobic respiration; FrdAB are the catalytic subcomplex consisting of a flavoprotein subunit and an iron-sulfur subunit, respectively; FrdCD are the membrane components which interact with quinone and are involved in electron transfer; the catalytic subunits are similar to succinate dehydrogenase SdhAB; fumarate reductase subunit C 4055625..4056020 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158450 YP_003038050.1 CDS ECBD_3878 NC_012947.1 4056031 4056390 D in conjunction with FrdC acts to anchor the catalytic components of the fumarate reductase to the cytoplasmic membrane; fumarate reductase subunit D 4056031..4056390 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158451 YP_003038051.1 CDS ampC NC_012947.1 4056453 4057586 D PFAM: beta-lactamase; KEGG: sdy:SDY_4394 beta-lactamase; beta-lactamase 4056453..4057586 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158452 YP_003038052.1 CDS ECBD_3880 NC_012947.1 4057675 4058211 D lipocalin; globomycin-sensitive outer membrane lipoprotein; outer membrane lipoprotein Blc 4057675..4058211 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158453 YP_003038053.1 CDS ECBD_3881 NC_012947.1 4058205 4058522 R member of the SMR family of proton-dependent drug efflux transporters; quaternary ammonium compound efflux pump; confers resistance to cetylpyridinium, cetyldimethylethyl ammonium and cetrimide cations; quaternary ammonium compound-resistance protein SugE complement(4058205..4058522) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158454 YP_003038054.1 CDS ECBD_3882 NC_012947.1 4058698 4058844 R PFAM: entericidin EcnAB; KEGG: sfv:SFV_4306 entericidin B membrane lipoprotein; entericidin B membrane lipoprotein complement(4058698..4058844) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158455 YP_003038055.1 CDS ECBD_3883 NC_012947.1 4058988 4059086 D hypothetical protein 4058988..4059086 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158456 YP_003038056.1 CDS ECBD_3884 NC_012947.1 4059132 4059698 R Involved in peptide bond synthesis; alters the affinity of the ribosome for aminoacyl-tRNA; elongation factor P complement(4059132..4059698) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158457 YP_003038057.1 CDS ECBD_3885 NC_012947.1 4059740 4060768 D KEGG: efe:EFER_4198 lysine aminomutase; TIGRFAM: lysine 2,3-aminomutase YodO family protein; PFAM: radical SAM protein; lysine 2,3-aminomutase YodO family protein 4059740..4060768 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158458 YP_003038058.1 CDS ECBD_3886 NC_012947.1 4061163 4062032 D KEGG: sbc:SbBS512_E4674 hypothetical protein; hypothetical protein 4061163..4062032 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158459 YP_003038059.1 CDS ECBD_3887 NC_012947.1 4062225 4062578 R KEGG: sdy:SDY_4450 hypothetical protein; hypothetical protein complement(4062225..4062578) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158460 YP_003038060.1 CDS groEL NC_012947.1 4062716 4064362 R 60 kDa chaperone family; promotes refolding of misfolded polypeptides especially under stressful conditions; forms two stacked rings of heptamers to form a barrel-shaped 14mer; ends can be capped by GroES; misfolded proteins enter the barrel where they are refolded when GroES binds; many bacteria have multiple copies of the groEL gene which are active under different environmental conditions; the B.japonicum protein in this cluster is expressed constitutively; in Rhodobacter, Corynebacterium and Rhizobium this protein is essential for growth; chaperonin GroEL complement(4062716..4064362) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158461 YP_003038061.1 CDS groES NC_012947.1 4064406 4064699 R 10 kDa chaperonin; Cpn10; GroES; forms homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring; co-chaperonin GroES complement(4064406..4064699) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158462 YP_003038062.1 CDS ECBD_3890 NC_012947.1 4064975 4066231 D uncharacterized member of the APC superfamily of amino acid transporters; unknown function; inner membrane protein YjeH 4064975..4066231 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158463 YP_003038063.1 CDS fxsA NC_012947.1 4066247 4066723 R F exclusion of bacteriophage T7; overproduction of this protein in Escherichia coli inhibits the F plasmid-mediated exclusion of bacteriophage T7; interacts with the F plasmid-encoded PifA protein; inner membrane protein; FxsA complement(4066247..4066723) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158464 YP_003038064.1 CDS aspA NC_012947.1 4067060 4068496 D catalyzes the formation of fumarate from aspartate; aspartate ammonia-lyase 4067060..4068496 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158465 YP_003038065.1 CDS ECBD_3893 NC_012947.1 4068614 4069915 D functions in anaerobic transport of C4-dicarboxylate compounds such as fumarate; similar to dcuB; anaerobic C4-dicarboxylate transporter 4068614..4069915 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158466 YP_003038066.1 CDS ECBD_3894 NC_012947.1 4070031 4070369 D copper binding protein required for copper tolerance; involved in resistance toward heavy metals; divalent-cation tolerance protein CutA 4070031..4070369 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158467 YP_003038067.1 CDS dipZ NC_012947.1 4070345 4072042 D two electrons are transferred from cytoplasmic NADPH to thioredoxin and then to the inner membrane protein DsbD which keeps the disulfide isomerase DsbC in a reduced state in the oxidizing periplasm; DsbC in turns rearranges incorrectly made disulfide bonds in the periplasm; thiol:disulfide interchange protein precursor 4070345..4072042 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158468 YP_003038068.1 CDS ECBD_3896 NC_012947.1 4072079 4072654 D PFAM: regulatory protein TetR; KEGG: ssn:SSON_4318 transcriptional regulator; transcriptional regulator 4072079..4072654 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158469 YP_003038069.1 CDS ECBD_3897 NC_012947.1 4073453 4074991 D regulates the cadBA operon; CadC family transcriptional regulator 4073453..4074991 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158471 YP_003038070.1 CDS cadB NC_012947.1 4075357 4076691 D antiporter protein responsible for lysine import and cadaverine export; member of the lysine-dependent acid resistance system 4 (AR4); inner membrane protein; lysine/cadaverine antiporter 4075357..4076691 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158472 YP_003038071.1 CDS ECBD_3899 NC_012947.1 4076771 4078918 D PFAM: Orn/Lys/Arg decarboxylase major region; Orn/Lys/Arg decarboxylase domain protein; lysine decarboxylase 4076771..4078918 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158473 YP_003038072.1 CDS ECBD_3900 NC_012947.1 4078977 4080434 D TIGRFAM: amino acid/peptide transporter; PFAM: TGF-beta receptor type I/II extracellular region; major facilitator superfamily MFS_1; KEGG: ssn:SSON_4307 peptide transporter; amino acid/peptide transporter 4078977..4080434 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158474 YP_003038073.1 CDS ECBD_3901 NC_012947.1 4080671 4082188 D class II; inducible; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; induced by high temperature, anaerobiosis, and low pH; in Methanosarcina barkeri, LysRS2 charges both tRNA molecules for lysine that exist in this organism and in addition can charge the tRNAPyl with lysine in the presence of LysRS1; lysyl-tRNA synthetase 4080671..4082188 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158475 YP_003038074.1 CDS ECBD_3902 NC_012947.1 4082307 4082480 R KEGG: elf:LF82_3446 uncharacterized protein YjdO; hypothetical protein complement(4082307..4082480) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158476 YP_003038075.1 CDS ECBD_3903 NC_012947.1 4082508 4082804 R KEGG: ssn:SSON_4305 hypothetical protein; hypothetical protein complement(4082508..4082804) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158477 YP_003038076.1 CDS ECBD_3904 NC_012947.1 4083031 4083303 R KEGG: sdy:SDY_4240 hypothetical protein; hypothetical protein complement(4083031..4083303) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158478 YP_003038077.1 CDS ECBD_3905 NC_012947.1 4083315 4083545 R PFAM: protein of unknown function DUF1271; hypothetical protein complement(4083315..4083545) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158479 YP_003038078.1 CDS ECBD_3907 NC_012947.1 4085359 4086078 D response regulator in two-component regulatory system with DcuS; phosphorylated DcuR activates transcription of genes involved in anaerobic fumarate respiration; DcuR family transcriptional regulator 4085359..4086078 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158481 YP_003038079.1 CDS ECBD_3908 NC_012947.1 4086649 4087989 D functions in anaerobic transport of C4-dicarboxylate compounds such as fumarate; similar to DcuA; DcuA and DcuB function as independent and mutually redundant C4-dicarboxylate (aspartate, malate, fumarate and succinate) transporters; anaerobic C4-dicarboxylate transporter 4086649..4087989 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158482 YP_003038080.1 CDS ECBD_3909 NC_012947.1 4088067 4089713 D TIGRFAM: hydro-lyase, Fe-S type, tartrate/fumarate subfamily subunit beta; hydro-lyase, Fe-S type, tartrate/fumarate subfamily, subunit alpha; PFAM: Fe-S type hydro-lyase tartrate/fumarate alpha region; Fe-S type hydro-lyase tartrate/fumarate beta region; KEGG: sdy:SDY_4231 fumarase B; hydro-lyase, Fe-S type, tartrate/fumarate subfamily, subunit beta 4088067..4089713 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158483 YP_003038081.1 CDS ECBD_3910 NC_012947.1 4089836 4090465 D KEGG: sbo:SBO_4325 hypothetical protein; hypothetical protein 4089836..4090465 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158484 YP_003038082.1 CDS ECBD_3911 NC_012947.1 4090604 4092013 R TIGRFAM: sugar (glycoside-Pentoside-Hexuronide) transporter; melibiose:sodium symporter complement(4090604..4092013) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158485 YP_003038083.1 CDS ECBD_3912 NC_012947.1 4092128 4093483 R PFAM: glycoside hydrolase family 4; glycoside hydrolase family 4 complement(4092128..4093483) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158486 YP_003038084.1 CDS ECBD_3913 NC_012947.1 4093766 4094674 D PFAM: helix-turn-helix- domain containing protein AraC type; SMART: helix-turn-helix- domain containing protein AraC type; DNA-binding transcriptional regulator MelR 4093766..4094674 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156586 YP_003038085.1 CDS ECBD_3914 NC_012947.1 4094870 4097140 D PFAM: Orn/Lys/Arg decarboxylase major region; Orn/Lys/Arg decarboxylase domain protein; Arginine decarboxylase 4094870..4097140 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156587 YP_003038086.1 CDS ECBD_3915 NC_012947.1 4097465 4098226 D PFAM: helix-turn-helix- domain containing protein AraC type; SMART: helix-turn-helix- domain containing protein AraC type; KEGG: sfv:SFV_4114 AraC-type regulatory protein; AraC family transcriptional regulator 4097465..4098226 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156588 YP_003038087.1 CDS ECBD_3916 NC_012947.1 4098363 4099700 D PFAM: amino acid permease-associated region; KEGG: ssn:SSON_4290 arginine:agmatin antiporter; arginine:agmatin antiporter 4098363..4099700 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156589 YP_003038088.1 CDS ECBD_3917 NC_012947.1 4099804 4101447 D with ZipA and an unidentified 24 kDa protein forms a complex involved in cell division; cell division protein 4099804..4101447 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156590 YP_003038089.1 CDS ECBD_3918 NC_012947.1 4101444 4102112 D response regulator in two-component regulatory system with BasS; DNA-binding transcriptional regulator BasR 4101444..4102112 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156591 YP_003038090.1 CDS ECBD_3919 NC_012947.1 4102113 4103213 D PFAM: ATP-binding region ATPase domain protein; histidine kinase HAMP region domain protein; histidine kinase A domain protein; SMART: ATP-binding region ATPase domain protein; histidine kinase A domain protein; KEGG: sfx:S3619 sensor protein BasS/PmrB; sensor protein BasS/PmrB 4102113..4103213 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156592 YP_003038091.1 CDS ECBD_3920 NC_012947.1 4103390 4104892 R TIGRFAM: metabolite/H+ symporter, major facilitator superfamily (MFS); PFAM: general substrate transporter; Osmosensory transporter coiled coil; major facilitator superfamily MFS_1; KEGG: sdy:SDY_4102 proline/glycine betaine transporter; proline/glycine betaine transporter complement(4103390..4104892) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156593 YP_003038092.1 CDS ECBD_3921 NC_012947.1 4105156 4106034 R KEGG: sfv:SFV_4120 hypothetical protein; hypothetical protein complement(4105156..4106034) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156594 YP_003038093.1 CDS ECBD_3922 NC_012947.1 4106031 4108259 R KEGG: sfv:SFV_4121 hypothetical protein; hypothetical protein complement(4106031..4108259) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156595 YP_003038094.1 CDS ECBD_3923 NC_012947.1 4108660 4108995 D TIGRFAM: alkylphosphonate utilization operon protein PhnA; PFAM: PhnA protein; KEGG: sfv:SFV_4122 hypothetical protein; hypothetical protein 4108660..4108995 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156596 YP_003038095.1 CDS ECBD_3924 NC_012947.1 4109553 4109996 D PFAM: glyoxalase/bleomycin resistance protein/dioxygenase; 3-demethylubiquinone-9 3-methyltransferase; KEGG: sfv:SFV_4123 hypothetical protein; hypothetical protein 4109553..4109996 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156597 YP_003038096.1 CDS ECBD_3925 NC_012947.1 4110129 4110917 D KEGG: ssn:SSON_4281 phosphonate/organophosphate ester transporter subunit; TIGRFAM: phosphonate ABC transporter ATPase subunit; PFAM: ABC transporter; SMART: ATPase AAA; phosphonate/organophosphate ester transporter subunit 4110129..4110917 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156598 YP_003038097.1 CDS ECBD_3926 NC_012947.1 4110942 4111958 D TIGRFAM: phosphonate ABC transporter periplasmic phosphonate binding protein; phosphonate ABC transporter periplasmic phosphonate-binding protein; KEGG: sbo:SBO_4130 periplasmic binding protein component of Pn transporter; phosphonate ABC transporter substrate-binding protein 4110942..4111958 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156599 YP_003038098.1 CDS ECBD_3927 NC_012947.1 4112064 4112843 D TIGRFAM: phosphonate ABC transporter inner membrane subunit; PFAM: binding-protein-dependent transporters inner membrane component; KEGG: sdy:SDY_4113 membrane channel protein component of Pn transporter; phosphonate ABC transporter permease 4112064..4112843 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156600 YP_003038099.1 CDS ECBD_3928 NC_012947.1 4112864 4113589 D may be involved in phosphonate uptake and biodegradation; phosphonate metabolism transcriptional regulator PhnF 4112864..4113589 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156601 YP_003038100.1 CDS ECBD_3929 NC_012947.1 4113590 4114042 D TIGRFAM: phosphonate C-P lyase system protein PhnG; PFAM: phosphonate metabolism PhnG; KEGG: ssn:SSON_4277 phosphonate metabolism; phosphonate C-P lyase system protein PhnG 4113590..4114042 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156602 YP_003038101.1 CDS phnH NC_012947.1 4114039 4114623 D TIGRFAM: phosphonate C-P lyase system protein PhnH; PFAM: phosphonate metabolism; KEGG: sdy:SDY_4116 carbon-phosphorus lyase complex subunit; carbon-phosphorus lyase complex subunit 4114039..4114623 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156603 YP_003038102.1 CDS ECBD_3931 NC_012947.1 4114623 4115687 D PFAM: phosphonate metabolism; KEGG: ssn:SSON_4275 phosphonate metabolism; phosphonate metabolism 4114623..4115687 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156604 YP_003038103.1 CDS ECBD_3932 NC_012947.1 4115680 4116525 D PFAM: phosphonate metabolism PhnJ; KEGG: sbo:SBO_4124 PhnJ; phosphonate metabolism PhnJ 4115680..4116525 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156605 YP_003038104.1 CDS phnK NC_012947.1 4116522 4117280 D KEGG: ssn:SSON_4273 phosphonates transport ATP-binding protein; TIGRFAM: phosphonate C-P lyase system protein PhnK; PFAM: ABC transporter related; SMART: ATPase AAA; phosphonate C-P lyase system protein PhnK 4116522..4117280 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156606 YP_003038105.1 CDS ECBD_3934 NC_012947.1 4117391 4118071 D KEGG: sdy:SDY_4120 ATP-binding component of phosphonate transport; TIGRFAM: phosphonate C-P lyase system protein PhnL; PFAM: ABC transporter related; SMART: ATPase AAA; phosphonate C-P lyase system protein PhnL 4117391..4118071 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156607 YP_003038106.1 CDS ECBD_3935 NC_012947.1 4118068 4119204 D TIGRFAM: phosphonate metabolism protein PhnM; PFAM: amidohydrolase; amidohydrolase; KEGG: ssn:SSON_4271 phosphonate metabolism; phosphonate metabolism protein PhnM 4118068..4119204 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156608 YP_003038107.1 CDS ECBD_3936 NC_012947.1 4119204 4119761 D TIGRFAM: phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN; SMART: guanylate kinase/L-type calcium channel region; KEGG: ssn:SSON_4270 ribose 1,5-bisphosphokinase; ribose 1,5-bisphosphokinase 4119204..4119761 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156609 YP_003038108.1 CDS ECBD_3937 NC_012947.1 4119748 4120182 D PhnO in Salmonella enterica catalyzes the acetylation of a range of aminoalkylphosphonic acids; part of the biochemical pathway that enables the cell to use phosphonates as a phosphorus source; Escherichia coli uses a different mechanism of phosphonate catabolism where PhnO is not essential and seems to play a regulatory role; aminoalkylphosphonic acid N-acetyltransferase 4119748..4120182 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156610 YP_003038109.1 CDS phnP NC_012947.1 4120184 4120942 D required for the use of phosphonates and phosphite as phosphorus sources; carbon-phosphorus lyase complex accessory protein 4120184..4120942 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156611 YP_003038110.1 CDS ECBD_3939 NC_012947.1 4121089 4121418 R KEGG: sbo:SBO_4117 hypothetical protein; hypothetical protein complement(4121089..4121418) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156612 YP_003038111.1 CDS ECBD_3940 NC_012947.1 4121487 4121936 R catalyzes the interconversion of ribose 5-phosphate to ribulose 5-phosphate; enzyme from E. coli shows allose 6-phosphate isomerase activity; ribose-5-phosphate isomerase B complement(4121487..4121936) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156613 YP_003038112.1 CDS ECBD_3941 NC_012947.1 4122295 4123185 D involved in rpiB and als operon repression; DNA-binding transcriptional repressor RpiR 4122295..4123185 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156614 YP_003038113.1 CDS ECBD_3942 NC_012947.1 4123244 4124179 D PFAM: periplasmic binding protein/LacI transcriptional regulator; KEGG: elf:LF82_0076 D-allose-binding periplasmic protein; D-allose transporter subunit 4123244..4124179 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156615 YP_003038114.1 CDS ECBD_3943 NC_012947.1 4124306 4125838 D with AlsBC acts to import allose into the cell; AlsA contain 2 ATP-binding domains; D-allose transporter ATP-binding protein 4124306..4125838 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156616 YP_003038115.1 CDS ECBD_3944 NC_012947.1 4125817 4126797 D PFAM: inner-membrane translocator; KEGG: elf:LF82_0077 D-allose transporter permease AlsC; D-allose transporter subunit 4125817..4126797 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156617 YP_003038116.1 CDS ECBD_3945 NC_012947.1 4126808 4127503 D PFAM: ribulose-phosphate 3-epimerase; KEGG: elf:LF82_0078 D-allulose-6-phosphate 3-epimerase; allulose-6-phosphate 3-epimerase 4126808..4127503 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156618 YP_003038117.1 CDS ECBD_3946 NC_012947.1 4127487 4128416 D PFAM: ROK family protein; KEGG: elf:LF82_0079 D-allose kinase; D-allose kinase 4127487..4128416 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156619 YP_003038118.1 CDS ECBD_3947 NC_012947.1 4128689 4130674 D PFAM: beta-lactamase; KEGG: elf:LF82_3435 uncharacterized protein YjcS; beta-lactamase 4128689..4130674 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156620 YP_003038119.1 CDS ECBD_3948 NC_012947.1 4130883 4131158 D KEGG: sbo:SBO_4114 formate dehydrogenase H; formate dehydrogenase H 4130883..4131158 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156621 YP_003038120.1 CDS ECBD_3949 NC_012947.1 4131177 4132208 D with MdtO and MdtP is involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride; multidrug resistance protein MdtN 4131177..4132208 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156622 YP_003038121.1 CDS ECBD_3950 NC_012947.1 4132208 4134259 D possibly part of a tripartite efflux system composed of MdtN, MdtO and MdtP which could be involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride; multidrug efflux system protein MdtO 4132208..4134259 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156623 YP_003038122.1 CDS ECBD_3951 NC_012947.1 4134256 4135722 D part of a multidrug efflux system involved in resistance to acriflavin, puromycin, erytjhromycin and tetraphenylarsonium chloride; member of the outer membrane factor (OMF) family; outer membrane efflux protein MdtP 4134256..4135722 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156624 YP_003038123.1 CDS ECBD_3952 NC_012947.1 4135920 4136339 D PFAM: molybdopterin oxidoreductase Fe4S4 region; KEGG: efe:EFER_4292 formate dehydrogenase-H, selenopolypeptide subunit; molybdopterin oxidoreductase Fe4S4 region 4135920..4136339 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156625 YP_003038124.1 CDS ECBD_3953 NC_012947.1 4136388 4138067 D TIGRFAM: formate dehydrogenase, subunit alpha; PFAM: molybdopterin oxidoreductase; molydopterin dinucleotide-binding region; formate dehydrogenase, subunit alpha 4136388..4138067 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156626 YP_003038125.1 CDS ECBD_3954 NC_012947.1 4138161 4138850 D PFAM: Sel1 domain protein repeat-containing protein; SMART: Sel1 domain protein repeat-containing protein; KEGG: sfv:SFV_4133 hypothetical protein; Sel1 domain protein repeat-containing protein 4138161..4138850 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156627 YP_003038126.1 CDS gltP NC_012947.1 4139267 4140580 R carrier protein part of the Na(+)-independent, binding-protein-independent glutamate-aspartate transporter; glutamate/aspartate:proton symporter complement(4139267..4140580) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156628 YP_003038127.1 CDS ECBD_3956 NC_012947.1 4140922 4141518 R PFAM: hypotheticalprotein; SMART: hypothetical protein; KEGG: sfv:SFV_4135 formate-dependent nitrite reductase complex subunit NrfG; formate-dependent nitrite reductase complex subunit NrfG complement(4140922..4141518) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156629 YP_003038128.1 CDS ECBD_3957 NC_012947.1 4141515 4141898 R TIGRFAM: cytochrome C nitrite reductase, accessory protein NrfF; PFAM: cytochrome C biogenesis protein; KEGG: sbc:SbBS512_E4596 formate-dependent nitrite reductase complex, NrfF subunit; formate-dependent nitrite reductase complex subunit NrfF complement(4141515..4141898) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156630 YP_003038129.1 CDS ECBD_3958 NC_012947.1 4141891 4143573 R with NrfF and NrfG catalyzes the insertion of heme into cytochrome C552; heme lyase subunit NrfE complement(4141891..4143573) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156631 YP_003038130.1 CDS ECBD_3959 NC_012947.1 4143629 4144585 R TIGRFAM: cytochrome C nitrite reductase, NrfD subunit; PFAM: polysulphide reductase NrfD; KEGG: ssn:SSON_4254 formate-dependent nitrate reductase complex transmembrane protein; cytochrome C nitrite reductase, NrfD subunit complement(4143629..4144585) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156632 YP_003038131.1 CDS ECBD_3960 NC_012947.1 4144582 4145253 R TIGRFAM: cytochrome C nitrite reductase, Fe-S protein; PFAM: 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: sfv:SFV_4139 formate-dependent nitrite reductase; cytochrome C nitrite reductase, Fe-S protein complement(4144582..4145253) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156633 YP_003038132.1 CDS ECBD_3961 NC_012947.1 4145250 4145816 R part of nitrite reductase complex; NrfB is active at high nitrate conditions; NrfA, B, C, and D are essential for the formate-dependent nitrite reduction to ammonia; cytochrome C nitrite reductase pentaheme subunit complement(4145250..4145816) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156634 YP_003038133.1 CDS nrfA NC_012947.1 4145861 4147297 R catalyzes the formate-dependent reduction of nitrite to ammonia; cytochrome C552; cytochrome C552 complement(4145861..4147297) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156635 YP_003038134.1 CDS ECBD_3963 NC_012947.1 4147690 4149648 D Acs; catalyzes the conversion of acetate and CoA to acetyl-CoA; acetyl-CoA synthetase 4147690..4149648 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156636 YP_003038135.1 CDS ECBD_3964 NC_012947.1 4149849 4150163 D PFAM: protein of unknown function DUF485; KEGG: sbc:SbBS512_E4587 hypothetical protein; hypothetical protein 4149849..4150163 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156637 YP_003038136.1 CDS actP NC_012947.1 4150160 4151809 D member of the sodium:solute symporter family; cotranscribed with the acs gene which encodes acetyl coenzyme A synthase; mutations affect acetate uptake; acetate permease 4150160..4151809 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156638 YP_003038137.1 CDS ECBD_3967 NC_012947.1 4153434 4155083 R TIGRFAM: Na+/H+ antiporter; PFAM: sodium/hydrogen exchanger; KEGG: sdy:SDY_4502 hypothetical protein; Na+/H+ antiporter complement(4153434..4155083) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156640 YP_003038138.1 CDS ECBD_3968 NC_012947.1 4155235 4156584 R PFAM: xanthine/uracil/vitamin C permease; sulfate transporter; KEGG: sdy:SDY_4503 hypothetical protein; xanthine/uracil/vitamin C permease complement(4155235..4156584) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156641 YP_003038139.1 CDS ECBD_3969 NC_012947.1 4157130 4157594 R KEGG: sdy:SDY_4504 redox-sensing activator of SoxS; TIGRFAM: redox-sensitive transcriptional activator SoxR; PFAM: transcription regulator MerR DNA binding; regulatory protein MerR; SMART: regulatory protein MerR; MerR family transcriptional regulator complement(4157130..4157594) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156642 YP_003038140.1 CDS ECBD_3970 NC_012947.1 4157680 4158003 D regulates genes involved in response to oxidative stress; DNA-binding transcriptional regulator SoxS 4157680..4158003 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156643 YP_003038141.1 CDS ECBD_3971 NC_012947.1 4158006 4159592 R PFAM: EAL domain protein; SMART: EAL domain protein; KEGG: sfv:SFV_4151 hypothetical protein; EAL domain protein complement(4158006..4159592) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156644 YP_003038142.1 CDS ECBD_3972 NC_012947.1 4160022 4160303 D KEGG: sbc:SbBS512_E4574 hypothetical protein; hypothetical protein 4160022..4160303 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156645 YP_003038143.1 CDS ECBD_3973 NC_012947.1 4160402 4160938 R binds to single stranded DNA and PriA helcase facilitate replication restart; single-stranded DNA-binding protein complement(4160402..4160938) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156646 YP_003038144.1 CDS uvrA NC_012947.1 4161193 4164015 D The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by uvrB, the uvrA molecules dissociate; excinuclease ABC subunit A 4161193..4164015 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156647 YP_003038145.1 CDS ECBD_3975 NC_012947.1 4164050 4164406 R PFAM: protein of unknown function DUF419; KEGG: sdy:SDY_4510 hypothetical protein; hypothetical protein complement(4164050..4164406) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156648 YP_003038146.1 CDS ECBD_3976 NC_012947.1 4164410 4164826 R PFAM: protein of unknown function UPF0047; KEGG: sdy:SDY_4511 hypothetical protein; hypothetical protein complement(4164410..4164826) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156649 YP_003038147.1 CDS aphA NC_012947.1 4164937 4165650 R class B; non-specific; catalyzes the dephosphorylation of organic phosphomonoesters; also has phosphotransferase activity; acid phosphatase/phosphotransferase complement(4164937..4165650) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156650 YP_003038148.1 CDS ECBD_3978 NC_012947.1 4166052 4166255 D KEGG: sfl:SF4150 hypothetical protein; hypothetical protein 4166052..4166255 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156651 YP_003038149.1 CDS ECBD_3979 NC_012947.1 4166757 4167950 R catalyzes the formation of L-glutamate and an aromatic oxo acid from an aromatic amino acid and 2-oxoglutarate; aromatic amino acid aminotransferase complement(4166757..4167950) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156652 YP_003038150.1 CDS alr NC_012947.1 4168203 4169282 R converts L-alanine to D-alanine which is used in cell wall biosynthesis; binds one pyridoxal phosphate per monomer; forms a homodimer; alanine racemase complement(4168203..4169282) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156653 YP_003038151.1 CDS ECBD_3981 NC_012947.1 4169335 4170750 R unwinds double stranded DNA; replicative DNA helicase complement(4169335..4170750) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156654 YP_003038152.1 CDS ECBD_3982 NC_012947.1 4170833 4171816 D PFAM: alcohol dehydrogenase zinc-binding domain protein; alcohol dehydrogenase GroES domain protein; KEGG: sdy:SDY_4523 quinone oxidoreductase, NADPH-dependent; quinone oxidoreductase, NADPH-dependent 4170833..4171816 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156655 YP_003038153.1 CDS pspG NC_012947.1 4171982 4172224 R coordinately regulated along with pspA; PspF-dependent induction in response to secretin overexpression in Yersinia; phage shock protein G complement(4171982..4172224) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156656 YP_003038154.1 CDS ECBD_3984 NC_012947.1 4172358 4173395 R TIGRFAM: TIM-barrel protein, yjbN family; PFAM: dihydrouridine synthase; KEGG: ssn:SSON_4229 tRNA-dihydrouridine synthase A; tRNA-dihydrouridine synthase A complement(4172358..4173395) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156657 YP_003038155.1 CDS ECBD_3985 NC_012947.1 4173758 4174504 R KEGG: sfv:SFV_4165 hypothetical protein; hypothetical protein complement(4173758..4174504) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156658 YP_003038156.1 CDS ECBD_3986 NC_012947.1 4174495 4174779 R KEGG: sfl:SF4158 hypothetical protein; hypothetical protein complement(4174495..4174779) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156659 YP_003038157.1 CDS ECBD_3987 NC_012947.1 4175098 4175613 D Acts as a negative controlling element, employing Zn(2+) as a cofactor to bind the operator of the repressed genes znuACB; zinc uptake transcriptional repressor 4175098..4175613 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156660 YP_003038158.1 CDS ECBD_3988 NC_012947.1 4175655 4175864 R unknown function; highly abundant protein in vivo; overexpressed under high NaCl concentrations; part of the sigma S regulon; non-essential; stress-response protein complement(4175655..4175864) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156661 YP_003038159.1 CDS ECBD_3989 NC_012947.1 4175980 4177305 R TIGRFAM: MATE efflux family protein; PFAM: multi antimicrobial extrusion protein MatE; KEGG: sfv:SFV_4169 DNA-damage-inducible SOS response protein; DNA-damage-inducible SOS response protein complement(4175980..4177305) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156662 YP_003038160.1 CDS ECBD_3990 NC_012947.1 4177378 4177986 R Represses a number of genes involved in the response to DNA damage; LexA repressor complement(4177378..4177986) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156663 YP_003038161.1 CDS ECBD_3991 NC_012947.1 4178096 4178464 R PFAM: diacylglycerol kinase; KEGG: sdy:SDY_4532 diacylglycerol kinase; diacylglycerol kinase complement(4178096..4178464) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158488 YP_003038162.1 CDS ECBD_3992 NC_012947.1 4178635 4181058 D PlsB; catalyzes the formation of 1-acyl-sn-glycerol 3-phosphate by transfering the acyl moiety from acyl-CoA; glycerol-3-phosphate acyltransferase 4178635..4181058 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158489 YP_003038163.1 CDS ubiA NC_012947.1 4181213 4182085 R catalyzes the conversion of 4-hydroxybenzoate into 3-octaprenyl-4-hydroxybenzoate, as part of the ubiquinone biosynthesis pathway; 4-hydroxybenzoate octaprenyltransferase complement(4181213..4182085) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158490 YP_003038164.1 CDS ubiC NC_012947.1 4182098 4182595 R catalyzes the formation of 4-hydroxybenzoate from chorismate; chorismate pyruvate lyase complement(4182098..4182595) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158491 YP_003038165.1 CDS ECBD_3995 NC_012947.1 4182818 4184146 R in E. coli, some yjbl mutations have been reported as suppressors of dnaG mutations; hypothetical protein complement(4182818..4184146) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156499 YP_003038166.1 CDS ECBD_3996 NC_012947.1 4184204 4184398 R hypothetical protein complement(4184204..4184398) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156500 YP_003038167.1 CDS ECBD_3997 NC_012947.1 4184500 4184652 D hypothetical protein 4184500..4184652 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156501 YP_003038168.1 CDS ECBD_3998 NC_012947.1 4184627 4185547 R PFAM: maltose operon periplasmic; maltose regulon periplasmic protein complement(4184627..4185547) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156502 YP_003038169.1 CDS lamB NC_012947.1 4185790 4187130 R porin involved in the transport of maltose and maltodextrins; maltoporin complement(4185790..4187130) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156503 YP_003038170.1 CDS ECBD_4000 NC_012947.1 4187202 4188317 R with malEFG is involved in import of maltose/maltodextrin; maltose/maltodextrin transporter ATP-binding protein complement(4187202..4188317) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156504 YP_003038171.1 CDS ECBD_4001 NC_012947.1 4188494 4188589 D hypothetical protein 4188494..4188589 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156505 YP_003038172.1 CDS malE NC_012947.1 4188682 4189872 D functions in the MalKFGE ABC transporter complex to transport maltose into the cell by using ATP hydrolysis; maltose ABC transporter periplasmic protein 4188682..4189872 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156506 YP_003038173.1 CDS malF NC_012947.1 4190026 4191570 D with MalKGE is involved in maltose transport into the cell; maltose transporter membrane protein 4190026..4191570 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156507 YP_003038174.1 CDS malG NC_012947.1 4191585 4192475 D with MalKFE is involved in the transport of maltose into the cell; maltose transporter permease 4191585..4192475 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156508 YP_003038175.1 CDS xylE NC_012947.1 4192847 4194322 D xylose/proton symporter; member of the major facilitator superfamily (MFS) of transporter; D-xylose transporter XylE 4192847..4194322 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156509 YP_003038176.1 CDS ECBD_4006 NC_012947.1 4194366 4194776 R PFAM: phosphate-starvation-inducible E; KEGG: sfx:S3556 phosphate-starvation-inducible protein PsiE; phosphate-starvation-inducible protein PsiE complement(4194366..4194776) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156510 YP_003038177.1 CDS ECBD_4007 NC_012947.1 4195046 4195324 D KEGG: sbo:SBO_4058 hypothetical protein; hypothetical protein 4195046..4195324 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156511 YP_003038178.1 CDS ECBD_4008 NC_012947.1 4195371 4197467 R PFAM: protein of unknown function DUF940 membrane lipoprotein KEGG: sbc:SbBS512_E4534 lipoprotein; hypothetical protein complement(4195371..4197467) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156512 YP_003038179.1 CDS ECBD_4009 NC_012947.1 4197467 4198204 R PFAM: protein of unknown function DUF1017; KEGG: sbc:SbBS512_E4533 hypothetical protein; hypothetical protein complement(4197467..4198204) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156513 YP_003038180.1 CDS ECBD_4010 NC_012947.1 4198201 4198839 R KEGG: efe:EFER_4119 lipoprotein; lipoprotein complement(4198201..4198839) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156514 YP_003038181.1 CDS ECBD_4011 NC_012947.1 4198954 4199196 R KEGG: elf:LF82_3422 uncharacterized protein YjbE; hypothetical protein complement(4198954..4199196) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156515 YP_003038182.1 CDS pgi NC_012947.1 4199695 4201344 R functions in sugar metabolism in glycolysis and the Embden-Meyerhof pathways (EMP) and in gluconeogenesis; catalyzes reversible isomerization of glucose-6-phosphate to fructose-6-phosphate; member of PGI family; glucose-6-phosphate isomerase complement(4199695..4201344) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156516 YP_003038183.1 CDS ECBD_4013 NC_012947.1 4201869 4203218 D catalyzes the formation of 4-phospho-L-aspartate from L-aspartate and ATP; functions in amino acid biosynthesis; lysine sensitive; aspartate kinase III 4201869..4203218 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156517 YP_003038184.1 CDS ECBD_4014 NC_012947.1 4203471 4203743 D KEGG: sdy:SDY_4309 hypothetical protein; hypothetical protein 4203471..4203743 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156518 YP_003038185.1 CDS ECBD_4015 NC_012947.1 4203876 4204748 R catalyzes the synthesis of pseudouridine from U-2604 in the 23S ribosomal RNA; 23S rRNA pseudouridine synthase F complement(4203876..4204748) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156519 YP_003038186.1 CDS ECBD_4016 NC_012947.1 4204960 4205649 D alpha-aspartyl dipeptidase; catalyzes the hydrolysis of dipeptides with an N-terminal aspartate residue; belongs to peptidase S51 family; peptidase E 4204960..4205649 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156520 YP_003038187.1 CDS ECBD_4017 NC_012947.1 4205740 4207371 R TIGRFAM: sodium-dependent inorganic phosphate (Pi) transporter; Na/Pi-cotransporter II-related protein; PFAM: Na+/Picotransporter; PhoU family protein; KEGG: ssn:SSON_4191 alpha helix protein; sodium-dependent inorganic phosphate (Pi) transporter complement(4205740..4207371) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156521 YP_003038188.1 CDS metH NC_012947.1 4207591 4211274 R one of two methionine synthases in Escherichia coli; MetH catalyzes a methyl transfer reaction from methyltetrahydrofolate to homocysteine to create methionine; requires zinc for activity; B12-dependent methionine synthase complement(4207591..4211274) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156522 YP_003038189.1 CDS ECBD_4019 NC_012947.1 4211474 4212298 D regulates the glyoxylate bypass operon (aceBAK), which encodes isocitrate lyase, malate synthase and isocitrate dehydrogenase kinase/phosphorylase; transcriptional repressor IclR 4211474..4212298 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156523 YP_003038190.1 CDS aceK NC_012947.1 4214158 4215894 R catalyzes the phosphorylation/dephosphorylation of the enzyme isocitrate dehydrogenase on a specific serine which regulates activity; unphosphorylated IDH is fully active when cells are grown on glucose while the enzyme becomes phosphorylated and inactive in the presence of acetate or ethanol; bifunctional isocitrate dehydrogenase kinase/phosphatase protein complement(4214158..4215894) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156525 YP_003038191.1 CDS ECBD_4022 NC_012947.1 4216175 4217479 R TIGRFAM: isocitrate lyase; PFAM: isocitrate lyase and phosphorylmutase; KEGG: sdy:SDY_4328 isocitrate lyase; isocitrate lyase complement(4216175..4217479) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156526 YP_003038192.1 CDS ECBD_4023 NC_012947.1 4217509 4219110 R Catalyzes the aldol condensation of glyoxylate with acetyl-CoA to form malate as part of the second step of the glyoxylate bypass and an alternative to the tricarboxylic acid cycle; malate synthase complement(4217509..4219110) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156527 YP_003038193.1 CDS ECBD_4024 NC_012947.1 4219379 4220308 R catalyzes the formation of O-succinyl-L-homoserine from succinyl-CoA and L-homoserine in methionine biosynthesis; homoserine O-succinyltransferase complement(4219379..4220308) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156528 YP_003038194.1 CDS ECBD_4025 NC_012947.1 4220465 4220908 D PFAM: GCN5-related N-acetyltransferase; hypothetical protein 4220465..4220908 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156529 YP_003038195.1 CDS purH NC_012947.1 4226916 4228505 D involved in de novo purine biosynthesis; bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase 4226916..4228505 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156534 YP_003038196.1 CDS ECBD_4027 NC_012947.1 4228517 4229806 D catalyzes the formation of N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-D-ribosylamine and glycine in purine biosynthesis; phosphoribosylamine--glycine ligase 4228517..4229806 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156535 YP_003038197.1 CDS ECBD_4028 NC_012947.1 4229803 4231128 R DNA-binding response regulator in two-component regulatory system with ZraS; response regulator/sigma54 interaction protein; transcriptional regulatory protein ZraR complement(4229803..4231128) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156536 YP_003038198.1 CDS ECBD_4029 NC_012947.1 4231125 4232501 R PFAM: ATP-binding region ATPase domain protein; histidine kinase A domain protein; SMART: ATP-binding region ATPase domain protein; histidine kinase A domain protein; KEGG: sfx:S3660 sensor protein ZraS; sensor protein ZraS complement(4231125..4232501) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156537 YP_003038199.1 CDS zraP NC_012947.1 4232739 4233158 D KEGG: elf:LF82_3728 zinc resistance-associated protein; zinc resistance protein 4232739..4233158 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156538 YP_003038200.1 CDS ECBD_4031 NC_012947.1 4233160 4233855 R PFAM: protein of unknown function DUF1481; KEGG: sfx:S3662 hypothetical protein; hypothetical protein complement(4233160..4233855) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156539 YP_003038201.1 CDS ECBD_4032 NC_012947.1 4233868 4234140 R histone-like DNA-binding protein; transcriptional regulator HU subunit alpha complement(4233868..4234140) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156540 YP_003038202.1 CDS ECBD_4033 NC_012947.1 4234327 4234917 R PFAM: protein of unknown function DUF416; KEGG: ssn:SSON_4172 hypothetical protein; hypothetical protein complement(4234327..4234917) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156541 YP_003038203.1 CDS ECBD_4034 NC_012947.1 4234960 4235631 R Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA; cleaves DNA containing urea residues, AP sites, base mismatches, insertion/deletion mismatches, flaps, and pseudo-Y structures; endonuclease V complement(4234960..4235631) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156542 YP_003038204.1 CDS hemE NC_012947.1 4235641 4236705 R catalyzes the formation of coproporphyrinogen from uroporphyrinogen III; uroporphyrinogen decarboxylase complement(4235641..4236705) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156543 YP_003038205.1 CDS nudC NC_012947.1 4236745 4237518 R can catalyze hydrolysis of broad range of dinucleotide pyrophosphates but prefers reduced form of NADH; requires divalent metal ions such as magnesium and manganese and produces two mononucleoside 5'-phosphates; NADH pyrophosphatase complement(4236745..4237518) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156544 YP_003038206.1 CDS ECBD_4037 NC_012947.1 4237613 4238089 D binds specifically to the major sigma factor sigma 70; active in stationary phase; anti-RNA polymerase sigma 70 factor 4237613..4238089 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156545 YP_003038207.1 CDS ECBD_4038 NC_012947.1 4238323 4240218 D required for the synthesis of the hydromethylpyrimidine moiety of thiamine; thiamine biosynthesis protein ThiC 4238323..4240218 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156546 YP_003038208.1 CDS ECBD_4039 NC_012947.1 4240218 4240853 D catalyzes the formation of thiamine monophosphate from 4-methyl-5-(beta-hydroxyethyl)-thiazole monophosphate and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate; thiamine-phosphate pyrophosphorylase 4240218..4240853 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156547 YP_003038209.1 CDS ECBD_4040 NC_012947.1 4240846 4241601 D TIGRFAM: thiazole biosynthesis adenylyltransferase ThiF; PFAM: UBA/THIF-type NAD/FAD binding protein; MoeZ/MoeB domain protein; KEGG: sdy:SDY_3734 thiamin biosynthesis, thiazole moiety; thiazole biosynthesis adenylyltransferase ThiF 4240846..4241601 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156548 YP_003038210.1 CDS ECBD_4041 NC_012947.1 4241585 4241785 D with ThiF, ThiG, and ThiO catalyzes the formation of the thiazole moiety of thiamine pyrophosphate; sulfur carrier protein ThiS 4241585..4241785 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156549 YP_003038211.1 CDS thiG NC_012947.1 4241787 4242557 D functions in thiamine (vitamin B1) biosynthesis; in Bacillus subtilis this enzyme catalyzes the formation of thiazole from dehydroxyglycine and 1-deoxy-D-xylulose-5-phosphate and ThiS-thiocarboxylate; thiazole synthase 4241787..4242557 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156550 YP_003038212.1 CDS thiH NC_012947.1 4242554 4243687 D in Escherichia coli this enzyme functions in thiamine biosynthesis along with thiFSGI and iscS; with ThiFSG catalyzes the formation of thiazole phosphate from tyrosine, cysteine and 1-deoxy-D-xylulose-5-phosphate; forms a complex with ThiG; contains an iron-sulfur center; thiamine biosynthesis protein ThiH 4242554..4243687 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156551 YP_003038213.1 CDS ECBD_4044 NC_012947.1 4244097 4244636 R KEGG: sbo:SBO_4009 heat shock protein; heat shock protein complement(4244097..4244636) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156552 YP_003038214.1 CDS ECBD_4045 NC_012947.1 4244849 4249072 R DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Subunit beta' binds to sigma factor allowing it to bind to the -10 region of the promoter; DNA-directed RNA polymerase subunit beta' complement(4244849..4249072) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156553 YP_003038215.1 CDS rpoB NC_012947.1 4249149 4253177 R DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates; subunit beta is part of the catalytic core which binds with a sigma factor to produce the holoenzyme; DNA-directed RNA polymerase subunit beta complement(4249149..4253177) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156554 YP_003038216.1 CDS rplL NC_012947.1 4253497 4253862 R present in two forms; L12 is normal, while L7 is aminoacylated at the N-terminal serine; the only multicopy ribosomal protein; 4:1 ratio of L7/L12 per ribosome; two L12 dimers bind L10; critically important for translation efficiency and fidelity; stimulates GTPase activity of translation factors; 50S ribosomal protein L7/L12 complement(4253497..4253862) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156555 YP_003038217.1 CDS rplJ NC_012947.1 4253929 4254426 R binds the two ribosomal protein L7/L12 dimers and anchors them to the large ribosomal subunit; 50S ribosomal protein L10 complement(4253929..4254426) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156556 YP_003038218.1 CDS rplA NC_012947.1 4254839 4255543 R in Escherichia coli and Methanococcus, this protein autoregulates expression; the binding site in the mRNA mimics the binding site in the 23S rRNA; 50S ribosomal protein L1 complement(4254839..4255543) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156557 YP_003038219.1 CDS rplK NC_012947.1 4255547 4255975 R binds directly to 23S ribosomal RNA; 50S ribosomal protein L11 complement(4255547..4255975) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156558 YP_003038220.1 CDS nusG NC_012947.1 4256134 4256679 R Modulates Rho-dependent transcription termination; transcription antitermination protein NusG complement(4256134..4256679) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156559 YP_003038221.1 CDS secE NC_012947.1 4256681 4257064 R forms a complex with SecY and SecG; SecYEG forms a protein-conducting channel to which secA binds and translocates targeted polypeptides across the cytoplasmic membrane, a process driven by ATP and a proton-motive force; preprotein translocase subunit SecE complement(4256681..4257064) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156560 YP_003038222.1 CDS ECBD_4053 NC_012947.1 4257294 4258478 R EF-Tu; promotes GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis; when the tRNA anticodon matches the mRNA codon, GTP hydrolysis results; the inactive EF-Tu-GDP leaves the ribosome and release of GDP is promoted by elongation factor Ts; many prokaryotes have two copies of the gene encoding EF-Tu; elongation factor Tu complement(4257294..4258478) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156561 YP_003038223.1 CDS ECBD_4054 NC_012947.1 4259396 4260346 D catalyzes the formation of (R)-4'-phosphopantothenate in coenzyme A biosynthesis; pantothenate kinase 4259396..4260346 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156566 YP_003038224.1 CDS ECBD_4055 NC_012947.1 4260375 4261340 R catalyzes the formation of biotinyl-5'-AMP, also acts as a transcriptional repressor of the biotin operon; biotin--protein ligase complement(4260375..4261340) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156567 YP_003038225.1 CDS murB NC_012947.1 4261337 4262365 R catalyzes the reduction of UDP-N-acetylglucosamine enolpyruvate to form UDP-N-acetylmuramate in peptidoglycan biosynthesis; UDP-N-acetylenolpyruvoylglucosamine reductase complement(4261337..4262365) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156568 YP_003038226.1 CDS ECBD_4057 NC_012947.1 4267886 4268743 R converts L-glutamate to D-glutamate, a component of peptidoglycan; glutamate racemase complement(4267886..4268743) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156573 YP_003038227.1 CDS ECBD_4059 NC_012947.1 4270901 4272001 D catalyzes the formation of 5-methyl-uridine at position 54 in all tRNAs; tRNA (uracil-5-)-methyltransferase 4270901..4272001 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156574 YP_003038228.1 CDS ECBD_4060 NC_012947.1 4272041 4272400 R PFAM: protein of unknown function DUF1422; KEGG: sbc:SbBS512_E4450 inner membrane protein; hypothetical protein complement(4272041..4272400) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156575 YP_003038229.1 CDS ECBD_4061 NC_012947.1 4272400 4273104 R negatively controls the expression of fabA and fabB, genes involved in the unsaturated fatty acid biosynthesis; DNA-binding transcriptional repressor FabR complement(4272400..4273104) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156576 YP_003038230.1 CDS ECBD_4062 NC_012947.1 4273381 4274781 D catalyzes the conversion of NADPH to NADH; soluble pyridine nucleotide transhydrogenase 4273381..4274781 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156577 YP_003038231.1 CDS ECBD_4063 NC_012947.1 4274764 4275681 R Activates the expression of a regulon of hydrogen peroxide-inducible genes such as katG, gor, ahpC, ahpF, oxyS, dps, fur and grxA; DNA-binding transcriptional regulator OxyR complement(4274764..4275681) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156578 YP_003038232.1 CDS ECBD_4064 NC_012947.1 4275948 4277321 R catalyzes the formation of arginine from (N-L-arginino)succinate; argininosuccinate lyase complement(4275948..4277321) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158492 YP_003038233.1 CDS ECBD_4065 NC_012947.1 4277382 4278158 R catalyzes the phosphorylation of N-acetyl-L-glutamate to form N-acetyl-L-glutamate 5-phosphate; acetylglutamate kinase complement(4277382..4278158) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158495 YP_003038234.1 CDS argC NC_012947.1 4278166 4279170 R catalyzes the reduction of N-acetyl-5-glutamyl phosphate to N-acetyl-L-glutamate 5-semialdehyde in arginine biosynthesis and the reduction of N-acetyl-gamma-aminoadipyl-phosphate to N-acetyl-L-aminoadipate-semialdehyde in lysine biosynthesis; involved in both the arginine and lysine biosynthetic pathways; lysine is produced via the AAA pathway, lysine from alpha-aminoadipate; N-acetyl-gamma-glutamyl-phosphate reductase complement(4278166..4279170) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158496 YP_003038235.1 CDS ECBD_4067 NC_012947.1 4279324 4280475 D catalyzes the formation of L-ornithine from N(2)-acetyl-L-ornithine in arginine biosynthesis; acetylornithine deacetylase 4279324..4280475 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158497 YP_003038236.1 CDS ECBD_4068 NC_012947.1 4280827 4283478 D catalyzes the formation of oxaloacetate from phosphoenolpyruvate; phosphoenolpyruvate carboxylase 4280827..4283478 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158498 YP_003038237.1 CDS ECBD_4069 NC_012947.1 4283660 4285393 D PFAM: sulfatase; KEGG: sbc:SbBS512_E4441 sulfatase; hypothetical protein 4283660..4285393 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158499 YP_003038238.1 CDS ECBD_4070 NC_012947.1 4285608 4286459 D PFAM: helix-turn-helix- domain containing protein AraC type; SMART: helix-turn-helix- domain containing protein AraC type; KEGG: sfv:SFV_4023 AraC-type regulatory protein; AraC family transcriptional regulator 4285608..4286459 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158500 YP_003038239.1 CDS ECBD_4071 NC_012947.1 4286446 4286787 R FrwD with FrwB, FrwC and PtsA forms a PEP-dependent sugar phosphotransferase system permease which may phosphorylate and transport sugars into the cell; phosphorylated by EIIA; FrwB homolog; fructose-like phosphotransferase EIIB subunit 3 complement(4286446..4286787) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158501 YP_003038240.1 CDS ECBD_4072 NC_012947.1 4286789 4287667 R KEGG: ssn:SSON_4125 pyruvate formate lyase II activase; TIGRFAM: glycyl-radical enzyme activating protein family; PFAM: radical SAM protein; pyruvate formate lyase II activase complement(4286789..4287667) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158502 YP_003038241.1 CDS pflD NC_012947.1 4287633 4289930 R involved in production of D-lactate from glucose under microaerobic conditions; cytoplasmic protein; formate acetyltransferase 2 complement(4287633..4289930) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158503 YP_003038242.1 CDS ECBD_4074 NC_012947.1 4289981 4290301 R FrwB with FrwC, FrwD and PtsA forms a PEP-dependent sugar phosphotransferase system (PTS) permease which may phosphorylate and transport sugars into the cell; cytoplasmic protein that interacts with complex EIIA; contains the second phosphorylation site of the PTS; PTS system fructose-like transporter subunit EIIB complement(4289981..4290301) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158504 YP_003038243.1 CDS ECBD_4075 NC_012947.1 4290316 4291395 R FrwC with FrwB, FrwD and PtsA forms a PEP-dependent sugar phosphotransferase system permease which may phosphorylate and transport sugars into the cell; forms translocation channel and contains the specific substrate-binding site; fructose-like permease EIIC subunit 2 complement(4290316..4291395) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158505 YP_003038244.1 CDS ECBD_4076 NC_012947.1 4291704 4294205 D TIGRFAM: phosphoenolpyruvate-protein phosphotransferase; PFAM: PEP-utilizing protein; PEP-utilising protein mobile region; PEP-utilising protein domain protein; phosphoryl transfer system HPr; phosphoenolpyruvate-dependent sugar phosphotransferase system EIIA 2; KEGG: elf:LF82_1767 multiphosphoryl transfer protein 2; phosphoenolpyruvate-protein phosphotransferase 4291704..4294205 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158506 YP_003038245.1 CDS ECBD_4077 NC_012947.1 4294217 4294879 D similar to transaldolase from Escherichia coli; many organisms have multiple copies; fructose-6-phosphate aldolase 4294217..4294879 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158507 YP_003038246.1 CDS gldA NC_012947.1 4294890 4295993 D forms dimers and octamers; involved in conversion of glycerol to dihydroxy-acetone; glycerol dehydrogenase 4294890..4295993 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158508 YP_003038247.1 CDS ECBD_4079 NC_012947.1 4296268 4296885 D PFAM: protein of unknown function DUF1287; KEGG: sbc:SbBS512_E4431 hypothetical protein; hypothetical protein 4296268..4296885 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158509 YP_003038248.1 CDS ECBD_4080 NC_012947.1 4296912 4297817 R PFAM: protein of unknown function DUF6 transmembrane; KEGG: elf:LF82_3413 uncharacterized inner membrane transporter yiJE; hypothetical protein complement(4296912..4297817) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158510 YP_003038249.1 CDS ECBD_4081 NC_012947.1 4297910 4300090 R TIGRFAM: catalase/peroxidase HPI; PFAM: Haem peroxidase; KEGG: sfv:SFV_4012 catalase; hydroperoxidase HPI(I); catalase/peroxidase HPI complement(4297910..4300090) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158511 YP_003038250.1 CDS metF NC_012947.1 4300419 4301309 R MTHFR; catalyzes NADH-linked reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate using FAD as a cofactor; 5,10-methylenetetrahydrofolate reductase complement(4300419..4301309) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158512 YP_003038251.1 CDS metL NC_012947.1 4301658 4304090 R multifunctional homodimeric enzyme that catalyzes the phosphorylation of aspartate to form aspartyl-4-phosphate as well as conversion of aspartate semialdehyde to homoserine; functions in a number of amino acid biosynthetic pathways; bifunctional aspartate kinase II/homoserine dehydrogenase II complement(4301658..4304090) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158513 YP_003038252.1 CDS ECBD_4084 NC_012947.1 4304093 4305253 R catalyzes the formation of cystathionine from L-cysteine and O-succinyl-L-homoserine; cystathionine gamma-synthase complement(4304093..4305253) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158514 YP_003038253.1 CDS ECBD_4085 NC_012947.1 4305530 4305847 D when combined with S-adenosylmethionine represses the expression of the methionine regulon and of proteins involved in S-adenosylmethionine synthesis; transcriptional repressor protein MetJ 4305530..4305847 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158515 YP_003038254.1 CDS ECBD_4086 NC_012947.1 4306031 4306639 D member of the NlpC/P60 superfamily of peptidases; peptidoglycan peptidase 4306031..4306639 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158516 YP_003038255.1 CDS ECBD_4087 NC_012947.1 4306643 4307269 D KEGG: efe:EFER_3835 conserved hypothetical protein; outer membrane lipoprotein; hypothetical protein 4306643..4307269 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158517 YP_003038256.1 CDS rpmE NC_012947.1 4307324 4307536 R RpmE; there appears to be two types of ribosomal proteins L31 in bacterial genomes; some contain a CxxC motif while others do not; Bacillus subtilis has both types; the proteins in this cluster have the CXXC motif; RpmE is found in exponentially growing Bacilli while YtiA was found after exponential growth; expression of ytiA is controlled by a zinc-specific transcriptional repressor; RpmE contains one zinc ion and a CxxC motif is responsible for this binding; forms an RNP particle along with proteins L5, L18, and L25 and 5S rRNA; found crosslinked to L2 and L25 and EF-G; may be near the peptidyltransferase site of the 50S ribosome; 50S ribosomal protein L31 complement(4307324..4307536) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158518 YP_003038257.1 CDS ECBD_4089 NC_012947.1 4307739 4309937 D binding of PriA to forked DNA starts the assembly of the primosome, also possesses 3'-5' helicase activity; primosome assembly protein PriA 4307739..4309937 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158519 YP_003038258.1 CDS ECBD_4090 NC_012947.1 4310093 4311118 D negatively controls the transcription initiation of genes such as deoCABD, udp, and cdd encoding catabolizing enzymes and nupC, nupG, and tsx encoding transporting and pore-forming proteins; DNA-binding transcriptional regulator CytR 4310093..4311118 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158520 YP_003038259.1 CDS ECBD_4091 NC_012947.1 4311210 4312169 D TIGRFAM: cell division protein FtsN; PFAM: sporulation domain protein; KEGG: sfl:SF4011 essential cell division protein; essential cell division protein FtsN 4311210..4312169 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158521 YP_003038260.1 CDS ECBD_4092 NC_012947.1 4312262 4312792 D heat shock protein involved in degradation of misfolded proteins; ATP-dependent protease peptidase subunit 4312262..4312792 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158522 YP_003038261.1 CDS hslU NC_012947.1 4312802 4314133 D heat shock protein involved in degradation of misfolded proteins; ATP-dependent protease ATP-binding subunit HslU 4312802..4314133 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158523 YP_003038262.1 CDS ECBD_4094 NC_012947.1 4314200 4315126 D catalyzes the formation of dimethylmenaquinone from 1,4-dihydroxy-2-naphthoate and octaprenyl diphosphate; 1,4-dihydroxy-2-naphthoate octaprenyltransferase 4314200..4315126 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158524 YP_003038263.1 CDS ECBD_4095 NC_012947.1 4315219 4315704 D regulator of RNase E; increases half-life and abundance of RNAs; interacts with RNase E possibly inhibiting catalytic activity; ribonuclease activity regulator protein RraA 4315219..4315704 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158525 YP_003038264.1 CDS ECBD_4096 NC_012947.1 4315789 4316028 R PFAM: protein of unknown function DUF904; KEGG: sfl:SF4006 hypothetical protein; hypothetical protein complement(4315789..4316028) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158526 YP_003038265.1 CDS ECBD_4097 NC_012947.1 4316460 4317305 D TIGRFAM: MIP family channel protein; PFAM: major intrinsic protein; KEGG: ssn:SSON_4096 facilitated diffusion of glycerol; MIP family channel protein 4316460..4317305 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158527 YP_003038266.1 CDS glpK NC_012947.1 4317328 4318836 D Converts glycerol and ADP to glycerol-3-phosphate and ADP; glycerol kinase 4317328..4318836 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158528 YP_003038267.1 CDS glpX NC_012947.1 4318842 4319981 D type II fructose 1,6-bisphosphatae; in Escherichia coli this protein forms a dimer and binds manganese; fructose 1,6-bisphosphatase II 4318842..4319981 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158529 YP_003038268.1 CDS ECBD_4100 NC_012947.1 4320078 4320824 D PFAM: oxidoreductase FAD/NAD(P)-binding domain protein; oxidoreductase FAD-binding domain protein; KEGG: sdy:SDY_3820 ferredoxin-NADP reductase; ferredoxin-NADP reductase 4320078..4320824 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158530 YP_003038269.1 CDS ECBD_4101 NC_012947.1 4320829 4321257 R with UspC and Usp E is involved in resistance to UV radiation; universal stress protein UspD complement(4320829..4321257) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158531 YP_003038270.1 CDS ECBD_4102 NC_012947.1 4321284 4321583 R PFAM: conserved hypothetical protein; KEGG: sfx:S3747 hypothetical protein; hypothetical protein complement(4321284..4321583) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158532 YP_003038271.1 CDS ECBD_4103 NC_012947.1 4321795 4322235 R PFAM: protein of unknown function DUF805; KEGG: ssn:SSON_4090 hypothetical protein; hypothetical protein complement(4321795..4322235) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158533 YP_003038272.1 CDS ECBD_4104 NC_012947.1 4322336 4322935 D PFAM: protein of unknown function DUF1454; KEGG: sfv:SFV_3574 hypothetical protein; hypothetical protein 4322336..4322935 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158534 YP_003038273.1 CDS tpiA NC_012947.1 4323043 4323810 D Reversibly isomerizes the ketone sugar dihydroxyacetone phosphate to the aldehyde sugar glyceraldehyde-3-phosphate; triosephosphate isomerase 4323043..4323810 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158535 YP_003038274.1 CDS ECBD_4106 NC_012947.1 4323865 4324620 R KEGG: sfv:SFV_3576 CDP-diacylglycerol pyrophosphatase; TIGRFAM: CDP-diacylglycerol pyrophosphatase; PFAM: CDP-diacylglycerol pyrophosphatase; CDP-diacylglycerol pyrophosphatase complement(4323865..4324620) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158536 YP_003038275.1 CDS ECBD_4107 NC_012947.1 4324727 4325716 R TIGRFAM: sulfate ABC transporter periplasmic sulfate-binding protein; PFAM: extracellular solute-binding protein family 1; KEGG: sfv:SFV_3577 sulfate transporter subunit; sulfate transporter subunit complement(4324727..4325716) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158537 YP_003038276.1 CDS ECBD_4108 NC_012947.1 4326036 4326998 R catalyzes the formation of D-fructose 1,6-bisphosphate from D-fructose 6-phosphate in glycolysis; 6-phosphofructokinase complement(4326036..4326998) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158538 YP_003038277.1 CDS fieF NC_012947.1 4327179 4328081 R member of cation diffusion facilitator family; CDF; membrane-bound; induced by both zinc and iron, but does not induce resistance to zinc; can transport zinc(II) in a proton-dependent manner; instead this protein induces iron resistance; forms dimers; ferrous iron efflux protein F complement(4327179..4328081) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158539 YP_003038278.1 CDS cpxP NC_012947.1 4328230 4328730 R repressor of the Cpx envelope stress response pathway which occurs via periplasmic interactions with CpxA; CpxP is degraded by DegP protease especially in the presence of misfolded substrates; periplasmic repressor CpxP complement(4328230..4328730) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158540 YP_003038279.1 CDS ECBD_4111 NC_012947.1 4328880 4329578 D response regulator in two-component regulatory system with CpxA; part of the envelope stress response system; DNA-binding transcriptional regulator CpxR 4328880..4329578 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158541 YP_003038280.1 CDS cpxA NC_012947.1 4329575 4330948 D part of two-component CpxA/CpxR system; senses envelope stress; upregulates a number of periplasmic folding and trafficking factors; two-component sensor protein 4329575..4330948 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158542 YP_003038281.1 CDS ECBD_4113 NC_012947.1 4331054 4331728 R PFAM: MOSC domain containing protein; 3-alpha domain protein; KEGG: sbo:SBO_3928 hypothetical protein; hypothetical protein complement(4331054..4331728) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158543 YP_003038282.1 CDS ECBD_4114 NC_012947.1 4331877 4332860 R transports degraded pectin products into the bacterial cell; 2-keto-3-deoxygluconate permease complement(4331877..4332860) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158544 YP_003038283.1 CDS ECBD_4115 NC_012947.1 4333120 4333740 R SodA; manganese binding; only present under aerobic conditions; destroys free radicals; superoxide dismutase complement(4333120..4333740) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158545 YP_003038284.1 CDS ECBD_4116 NC_012947.1 4334025 4335059 D transports L-rhamnose and L-lyxose into the cell; rhamnose-proton symporter 4334025..4335059 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158546 YP_003038285.1 CDS ECBD_4117 NC_012947.1 4335056 4335994 R activates the expression of rhaRS in response to L-rhamnose; transcriptional activator RhaR complement(4335056..4335994) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158547 YP_003038286.1 CDS ECBD_4118 NC_012947.1 4335978 4336814 R activates the expression of the rhaBAD operon and rhaT gene; transcriptional activator RhaS complement(4335978..4336814) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158548 YP_003038287.1 CDS ECBD_4119 NC_012947.1 4336874 4337023 R KEGG: sbc:SbBS512_E4383 hypothetical protein; hypothetical protein complement(4336874..4337023) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158549 YP_003038288.1 CDS rhaB NC_012947.1 4337102 4338571 D catalyzes the ATP-dependent phosphorylation of rhamnulose; rhamnulokinase 4337102..4338571 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158550 YP_003038289.1 CDS ECBD_4121 NC_012947.1 4338568 4339827 D catalyzes the formation of L-rhamnulose from L-rhamnose; L-rhamnose isomerase 4338568..4339827 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158551 YP_003038290.1 CDS ECBD_4122 NC_012947.1 4340002 4340826 D TIGRFAM: rhamnulose-1-phosphate aldolase; KEGG: sdy:SDY_3844 rhamnulose-1-phosphate aldolase; rhamnulose-1-phosphate aldolase 4340002..4340826 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158552 YP_003038291.1 CDS ECBD_4123 NC_012947.1 4340836 4341150 D TIGRFAM: L-rhamnose 1-epimerase; KEGG: sdy:SDY_3845 hypothetical protein; L-rhamnose 1-epimerase 4340836..4341150 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158553 YP_003038292.1 CDS ECBD_4124 NC_012947.1 4341191 4342585 R KEGG: elf:LF82_p621 glycoporin; glycoporin complement(4341191..4342585) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158554 YP_003038293.1 CDS ECBD_4125 NC_012947.1 4343062 4343508 D with FrvBRX is part of the phosphoenolpyruvate-dependent sugar phosphotransferase system involved in the transport and phosphorylation of sugars; possibly responsible for the sugar specificity of the system; fructose-like phosphotransferase system subunit EIIA 4343062..4343508 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158555 YP_003038294.1 CDS ECBD_4126 NC_012947.1 4343519 4344970 D TIGRFAM: PTS system, fructose subfamily, IIC subunit; PTS system, fructose-specific, IIB subunnit; KEGG: sbo:SBO_3915 PTS transporter components IIBC; PTS system transporter subunits IIBC 4343519..4344970 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158556 YP_003038295.1 CDS ECBD_4127 NC_012947.1 4344960 4346030 D with FrvABR is part of the phosphoenolpyruvate-dependent sugar phosphotransferase system which may be involved in the transport and phosphorylation of sugars; fructose-specific phosphotransferase system protein FrvX 4344960..4346030 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158557 YP_003038296.1 CDS ECBD_4128 NC_012947.1 4346030 4347778 D repressor of the frv operon, involved in phosphoenolpyruvate:sugar phosphotransferase system; frv operon regulatory protein 4346030..4347778 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158558 YP_003038297.1 CDS ECBD_4129 NC_012947.1 4347828 4348883 R hypothetical protein complement(4347828..4348883) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158559 YP_003038298.1 CDS ECBD_4130 NC_012947.1 4349036 4349869 R involved in the production or activity of formate dehydrogenase-H which is active when nitrate is not present during anaerobic growth; formate dehydrogenase accessory protein complement(4349036..4349869) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158560 YP_003038299.1 CDS ECBD_4131 NC_012947.1 4350063 4353113 D TIGRFAM: formate dehydrogenase, subunit alpha; PFAM: molybdopterin oxidoreductase; molybdopterin oxidoreductase Fe4S4 region; molydopterin dinucleotide-binding region; KEGG: ssn:SSON_4063 formate dehydrogenase-O, major subunit; formate dehydrogenase, subunit alpha 4350063..4353113 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158561 YP_003038300.1 CDS ECBD_4132 NC_012947.1 4353126 4354028 D TIGRFAM: formate dehydrogenase subunit beta; PFAM: formate dehydrogenase transmembrane domain protein; 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: sfv:SFV_3602 formate dehydrogenase-O, iron-sulfur subunit; formate dehydrogenase subunit beta 4353126..4354028 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158562 YP_003038301.1 CDS ECBD_4133 NC_012947.1 4354025 4354660 D cytochrome b556(FDO) component; heme containing; formate dehydrogenase-O subunit gamma 4354025..4354660 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158563 YP_003038302.1 CDS ECBD_4134 NC_012947.1 4354657 4355586 D required for the formation of active formate dehydrogenase; formate dehydrogenase accessory protein FdhE 4354657..4355586 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158564 YP_003038303.1 CDS ECBD_4135 NC_012947.1 4355734 4355916 R KEGG: efe:EFER_2513 4'-phosphopantetheinyl transferase EntD; hypothetical protein complement(4355734..4355916) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158565 YP_003038304.1 CDS ECBD_4136 NC_012947.1 4355916 4356158 R KEGG: sbc:SbBS512_E4369 hypothetical protein; hypothetical protein complement(4355916..4356158) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158566 YP_003038305.1 CDS ECBD_4137 NC_012947.1 4356376 4356594 R PFAM: CopG domain protein DNA-binding domain protein; KEGG: sfv:SFV_3606 hypothetical protein; CopG family transcriptional regulator complement(4356376..4356594) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158567 YP_003038306.1 CDS ECBD_4138 NC_012947.1 4356929 4357036 R hypothetical protein complement(4356929..4357036) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158568 YP_003038307.1 CDS ECBD_4139 NC_012947.1 4357447 4358388 R TIGRFAM: thioesterase domain protein; PFAM: GCN5-related N-acetyltransferase; KEGG: sbo:SBO_3902 acetyltransferase; thioesterase domain protein complement(4357447..4358388) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158569 YP_003038308.1 CDS ECBD_4140 NC_012947.1 4358433 4358870 R hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr); defense mechanism against a harmful effect of D-tyrosine; D-tyrosyl-tRNA(Tyr) deacylase complement(4358433..4358870) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158570 YP_003038309.1 CDS rbn NC_012947.1 4358867 4359739 R RNase BN; required for 3' maturation of certain phage T4-encoded tRNAs; forms a dimer; specific for immature tRNA substrates containing incorrect residues within the universal CCA sequence; 3' to 5' exoribonuclease; ribonuclease BN complement(4358867..4359739) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158571 YP_003038310.1 CDS ECBD_4142 NC_012947.1 4359733 4360332 R TIGRFAM: HAD-superfamily hydrolase, subfamily IA, variant 3; PFAM: haloacid dehalogenase; KEGG: ssn:SSON_4054 phosphatase; phosphatase complement(4359733..4360332) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158572 YP_003038311.1 CDS ECBD_4143 NC_012947.1 4360431 4361216 R PFAM: regulatory protein DeoR; Helix-turn-helix type 11 domain protein; SMART: regulatory protein DeoR; DeoR family transcriptional regulator complement(4360431..4361216) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158573 YP_003038312.1 CDS ECBD_4144 NC_012947.1 4361250 4362146 R PFAM: PfkB domain protein; KEGG: sfx:S3791 kinase; PfkB domain protein complement(4361250..4362146) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158574 YP_003038313.1 CDS ECBD_4145 NC_012947.1 4362314 4363210 D PFAM: 6-phosphogluconate dehydrogenase NAD-binding; KEGG: sfv:SFV_3617 dehydrogenase; 3-hydroxyisobutyrate dehydrogenase 4362314..4363210 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158575 YP_003038314.1 CDS ECBD_4146 NC_012947.1 4363234 4364112 D PFAM: deoxyribose-phosphate aldolase/phospho-2-dehydro-3-deoxyheptonate aldolase; KEGG: sfx:S3793 aldolase; deoxyribose-phosphate aldolase/phospho-2-dehydro-3-deoxyheptonate aldolase 4363234..4364112 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157825 YP_003038315.1 CDS ECBD_4147 NC_012947.1 4364129 4365370 D PFAM: N-acylglucosamine 2-epimerase; KEGG: sfx:S3794 hypothetical protein; N-acylglucosamine 2-epimerase 4364129..4365370 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157829 YP_003038316.1 CDS ECBD_4148 NC_012947.1 4365484 4366410 D KEGG: sfv:SFV_3620 aldose-1-epimerase; aldose-1-epimerase 4365484..4366410 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157830 YP_003038317.1 CDS ECBD_4149 NC_012947.1 4366535 4368538 D KEGG: sfv:SFV_3621 alpha-glucosidase; alpha-glucosidase 4366535..4368538 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157831 YP_003038318.1 CDS ECBD_4150 NC_012947.1 4368584 4369969 D TIGRFAM: sugar (glycoside-Pentoside-Hexuronide) transporter; KEGG: sfv:SFV_3622 permease; sugar (glycoside-Pentoside-Hexuronide) transporter 4368584..4369969 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157832 YP_003038319.1 CDS ECBD_4151 NC_012947.1 4370009 4371412 D TIGRFAM: sugar (glycoside-Pentoside-Hexuronide) transporter; sugar (glycoside-Pentoside-Hexuronide) transporter 4370009..4371412 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157833 YP_003038320.1 CDS ompL NC_012947.1 4371479 4372171 D porin involved in the transport of small molecular weight solutes (up to 600 Daltons) across the cell wall; specific substrate still unknown; outer membrane porin L 4371479..4372171 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157834 YP_003038321.1 CDS ECBD_4153 NC_012947.1 4372262 4373527 R Sugar phosphate permease-like protein complement(4372262..4373527) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157835 YP_003038322.1 CDS ECBD_4154 NC_012947.1 4373629 4374609 R KEGG: sfx:S3803 hypothetical protein; hypothetical protein complement(4373629..4374609) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157836 YP_003038323.1 CDS ECBD_4155 NC_012947.1 4374617 4375327 R SMART: regulatory protein GntR HTH; KEGG: sfv:SFV_3628 transcriptional regulator; GntR family transcriptional regulator complement(4374617..4375327) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157837 YP_003038324.1 CDS ECBD_4156 NC_012947.1 4375544 4377367 R TIGRFAM: GTP-binding protein TypA; small GTP-binding protein; KEGG: sdy:SDY_3872 GTP-binding protein; GTP-binding protein complement(4375544..4377367) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157838 YP_003038325.1 CDS glnA NC_012947.1 4377740 4379149 D forms a homododecamer; forms glutamine from ammonia and glutamate with the conversion of ATP to ADP and phosphate; also functions in the assimilation of ammonia; highly regulated protein controlled by the addition/removal of adenylyl groups by adenylyltransferase from specific tyrosine residues; addition of adenylyl groups results in inactivation of the enzyme; glutamine synthetase 4377740..4379149 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157839 YP_003038326.1 CDS glnL NC_012947.1 4379435 4380484 D sensory histidine kinase in two-component regulatory system with GlnG; acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC by phosphorylation/dephosphorylation; nitrogen regulation protein NR(II) 4379435..4380484 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157840 YP_003038327.1 CDS glnG NC_012947.1 4380496 4381905 D response regulator of a two-component regulatory system involved in the activation of nitrogen assimilation genes; interacts with sigma-54; nitrogen regulation protein NR(I) 4380496..4381905 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157841 YP_003038328.1 CDS ECBD_4160 NC_012947.1 4382017 4382127 D KEGG: ses:SARI_03655 hypothetical protein; hypothetical protein 4382017..4382127 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157842 YP_003038329.1 CDS ECBD_4161 NC_012947.1 4382317 4383690 R catalyzes the oxygen-independent formation of protoporphyrinogen-IX from coproporphyrinogen-III; coproporphyrinogen III oxidase complement(4382317..4383690) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157843 YP_003038330.1 CDS ECBD_4162 NC_012947.1 4383879 4384388 R PFAM: protein of unknown function DUF414; KEGG: sfl:SF3936 hypothetical protein; hypothetical protein complement(4383879..4384388) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157844 YP_003038331.1 CDS engB NC_012947.1 4384970 4385602 D binds guanine nucleotides; in Escherichia coli depletion results in defective cell division and filamentation; in Bacillus subtilis this gene is essential; ribosome biogenesis GTP-binding protein YsxC 4384970..4385602 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157845 YP_003038332.1 CDS ECBD_4164 NC_012947.1 4385616 4385765 D KEGG: sbc:SbBS512_E4338 hypothetical protein; hypothetical protein 4385616..4385765 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157846 YP_003038333.1 CDS ECBD_4165 NC_012947.1 4385983 4388769 R has 3'-5' exonuclease, 5'-3' exonuclease and 5'-3'polymerase activities, primarily functions to fill gaps during DNA replication and repair; DNA polymerase I complement(4385983..4388769) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157847 YP_003038334.1 CDS ECBD_4166 NC_012947.1 4389133 4390065 D PFAM: phospholipid/glycerol acyltransferase; SMART: phospholipid/glycerol acyltransferase; KEGG: ssn:SSON_4035 endonuclease; acyltransferase 4389133..4390065 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157848 YP_003038335.1 CDS ECBD_4167 NC_012947.1 4390106 4391536 R PFAM: protein of unknown function DUF945; KEGG: sfx:S3815 hypothetical protein; hypothetical protein complement(4390106..4391536) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157849 YP_003038336.1 CDS ECBD_4168 NC_012947.1 4391526 4391666 R KEGG: sbc:SbBS512_E4333 hypothetical protein; hypothetical protein complement(4391526..4391666) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157850 YP_003038337.1 CDS ECBD_4170 NC_012947.1 4391870 4393078 R PFAM: transposase IS4 family protein; KEGG: sea:SeAg_B3181 IS10 transposase; transposase IS4 family protein complement(4391870..4393078) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157852 YP_003038338.1 CDS ECBD_4171 NC_012947.1 4393672 4394658 R catalyzes the phosphorylation of protein substrates at serine and threonine residues in vitro; specific substrate in vivo has not been identified yet; plays a role in long-term cell survival and expression of surface appendages; serine/threonine protein kinase complement(4393672..4394658) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157853 YP_003038339.1 CDS ECBD_4172 NC_012947.1 4394735 4395004 R PFAM: protein of unknown function DUF1040; KEGG: sdy:SDY_3887 hypothetical protein; hypothetical protein complement(4394735..4395004) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157854 YP_003038340.1 CDS mobA NC_012947.1 4395074 4395658 D in Escherichia coli MobA links a guanosine 5'-phosphate to molydopterin to form molybdopterin guanine dinucleotide during molybdenum cofactor biosynthesis; molybdopterin-guanine dinucleotide biosynthesis protein MobA 4395074..4395658 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157855 YP_003038341.1 CDS ECBD_4174 NC_012947.1 4395640 4396167 D in Escherichia coli the MobB protein is not essential but has been found to interact with multiple proteins involved in the molybdopterin guanine dinucleotide cofactor biosynthesis pathway; molybdopterin-guanine dinucleotide biosynthesis protein B 4395640..4396167 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157856 YP_003038342.1 CDS hemG NC_012947.1 4401844 4402389 R catalyzes the oxidation of protoporphyrinogen IX to form protoporphyrin IX; protoporphyrinogen oxidase complement(4401844..4402389) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157862 YP_003038343.1 CDS ECBD_4176 NC_012947.1 4402401 4403852 R TIGRFAM: potassium uptake protein, TrkH family; PFAM: cation transporter; KEGG: sdy:SDY_3896 potassium transporter; potassium transporter complement(4402401..4403852) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157863 YP_003038344.1 CDS ECBD_4177 NC_012947.1 4403891 4404505 R PFAM: protein of unknown function UPF0029; Domain of unknown function DUF1949; KEGG: ssn:SSON_4021 hypothetical protein; hypothetical protein complement(4403891..4404505) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157864 YP_003038345.1 CDS ECBD_4178 NC_012947.1 4404505 4405836 R catalyzes the hydrolysis of dipeptides with a proline at the C-terminal; also catalyzes the low efficiency hydrolysis of organophosphate di- and tri-esters; proline dipeptidase complement(4404505..4405836) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157865 YP_003038346.1 CDS fadB NC_012947.1 4406026 4408215 D includes enoyl-CoA hydratase, delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase, 3-hydroxyacyl-CoA dehydrogenase, and 3-hydroxybutyryl-CoA epimerase; catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA; also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities; forms a heterotetramer with FadA; similar to FadI2J2 complex; functions in beta-oxidation of fatty acids; multifunctional fatty acid oxidation complex subunit alpha 4406026..4408215 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157866 YP_003038347.1 CDS fadA NC_012947.1 4408225 4409388 D FadA; fatty acid oxidation complex component beta; functions in a heterotetramer with FadB; similar to FadI2J2 complex; functions in beta-oxidation of fatty acids; 3-ketoacyl-CoA thiolase 4408225..4409388 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157867 YP_003038348.1 CDS fre NC_012947.1 4409671 4410372 R NAD(P)H-flavin reductase; catalyzes the reversible oxidation/reduction of NAD(P) and flavine mononucleotide; in Salmonella and E. coli this protein also reduces aquacob(III)alamin to cob(II)alamin; FMN reductase complement(4409671..4410372) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157868 YP_003038349.1 CDS ECBD_4182 NC_012947.1 4410418 4411911 R catalyzes the decarboxylation of 3-octaprenyl-4-hydroxy benzoate to 2-octaprenylphenol; 3-octaprenyl-4-hydroxybenzoate decarboxylase complement(4410418..4411911) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157869 YP_003038350.1 CDS rfaH NC_012947.1 4412078 4412566 D KEGG: sdy:SDY_3903 transcriptional activator RfaH; TIGRFAM: transcriptional activator RfaH; PFAM: NGN domain protein; SMART: NGN domain protein; transcriptional activator RfaH 4412078..4412566 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157870 YP_003038351.1 CDS ECBD_4184 NC_012947.1 4412563 4413345 R magnesium dependent; not involved in the Sec-independent protein export system; DNase TatD complement(4412563..4413345) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157871 YP_003038352.1 CDS ECBD_4185 NC_012947.1 4413387 4414163 R with TatABE forms the twin-arginine translocation complex which is involved in the transport of proteins across the cytoplasmic membrane; twin-arginine protein translocation system subunit TatC complement(4413387..4414163) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157872 YP_003038353.1 CDS ECBD_4186 NC_012947.1 4414166 4414681 R mediates the export of protein precursors bearing twin-arginine signal peptides; sec-independent translocase complement(4414166..4414681) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157873 YP_003038354.1 CDS tatA NC_012947.1 4414685 4414954 R TatA; similar to TatE that is found in some proteobacteria; part of system that translocates proteins with a conserved twin arginine motif across the inner membrane; capable of translocating folded substrates typically those with bound cofactors; similar to a protein import system in thylakoid membranes; twin arginine translocase protein A complement(4414685..4414954) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157874 YP_003038355.1 CDS ubiB NC_012947.1 4415033 4416673 R an Escherichia coli mutant results in accumulation of octaprenylphenol and no ubiquinone; functions in the formation of 2-octaprenyl-6-hydroxy-phenol from 2-octaprenylphenol in ubiquinone (coenzyme Q) biosynthesis; similar to eukaryotic proteins that exhibit kinase functions; ubiquinone biosynthesis protein UbiB complement(4415033..4416673) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157875 YP_003038356.1 CDS ECBD_4189 NC_012947.1 4416670 4417275 R PFAM: sterol-binding domain protein; KEGG: sbc:SbBS512_E4306 hypothetical protein; sterol-binding domain protein complement(4416670..4417275) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157876 YP_003038357.1 CDS ubiE NC_012947.1 4417289 4418044 R Catalyzes the carbon methylation reaction in the biosynthesis of ubiquinone; ubiquinone/menaquinone biosynthesis methyltransferase complement(4417289..4418044) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157877 YP_003038358.1 CDS ECBD_4191 NC_012947.1 4418139 4419566 R YigN; nuclease that may cleave DNA structures arising during the recombination of short-inverted repeats and thereby prevents the inversion of the internal sequence; transcription is induced by DNA-damaging agents such as nalidixic acid or mitomycin C in a LexA-dependent manner; DNA recombination protein RmuC complement(4418139..4419566) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157878 YP_003038359.1 CDS ECBD_4192 NC_012947.1 4419783 4420550 D PFAM: regulatory protein IclR; transcriptional regulator IclR; SMART: regulatory protein IclR; KEGG: elf:LF82_p601 transcriptional regulator; transcriptional regulator, IclR family 4419783..4420550 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157879 YP_003038360.1 CDS ECBD_4193 NC_012947.1 4420574 4421578 D PFAM: 2-keto-3-deoxy-galactonokinase; KEGG: elf:LF82_p600 hypothetical protein; 2-dehydro-3-deoxygalactonokinase 4420574..4421578 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157880 YP_003038361.1 CDS ECBD_4194 NC_012947.1 4421568 4422197 D catalyzes the formation of D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-galactonate 6-phosphate; functions in galactonate metabolism; 2-dehydro-3-deoxy-6-phosphogalactonate aldolase 4421568..4422197 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157881 YP_003038362.1 CDS ECBD_4195 NC_012947.1 4422222 4423196 D PFAM: conserved hypothetical protein; KEGG: elf:LF82_p598 hypothetical protein; hypothetical protein 4422222..4423196 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157882 YP_003038363.1 CDS ECBD_4196 NC_012947.1 4423213 4423719 D KEGG: elf:LF82_p597 hypothetical protein; hypothetical protein 4423213..4423719 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157883 YP_003038364.1 CDS ECBD_4197 NC_012947.1 4423732 4425222 D PFAM: protein of unknown function DUF112 transmembrane; KEGG: elf:LF82_p596 hypothetical protein; hypothetical protein 4423732..4425222 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157884 YP_003038365.1 CDS ECBD_4198 NC_012947.1 4425256 4426020 R catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate; involved in the pyrimidine salvage pathway; uridine phosphorylase complement(4425256..4426020) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157885 YP_003038366.1 CDS ECBD_4199 NC_012947.1 4426282 4427091 D PFAM: dienelactone hydrolase; KEGG: elf:LF82_3701 carboxymethylenebutenolidase; carboxymethylenebutenolidase 4426282..4427091 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157886 YP_003038367.1 CDS ECBD_4200 NC_012947.1 4427122 4427217 R hypothetical protein complement(4427122..4427217) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157887 YP_003038368.1 CDS ECBD_4201 NC_012947.1 4427348 4428016 D PFAM: isochorismatase hydrolase; KEGG: elf:LF82_p595 hypothetical protein, hypothetica l p rotein; isochorismatase hydrolase 4427348..4428016 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157888 YP_003038369.1 CDS ECBD_4202 NC_012947.1 4428022 4428882 D KEGG: elf:LF82_p594 hypothetical protein; hypothetical protein 4428022..4428882 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157889 YP_003038370.1 CDS ECBD_4203 NC_012947.1 4428977 4430536 D PFAM: FdrA family protein; KEGG: elf:LF82_p593 hypothetical protein; FdrA family protein 4428977..4430536 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157890 YP_003038371.1 CDS ECBD_4204 NC_012947.1 4430533 4431957 D PFAM: protein of unknown function DUF1116; KEGG: elf:LF82_p592 hypothetical protein; hypothetical protein 4430533..4431957 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157891 YP_003038372.1 CDS ECBD_4205 NC_012947.1 4431950 4432906 D catalyzes the reversible synthesis of carbamate and ATP from carbamoyl phosphate and ADP; carbamate kinase 4431950..4432906 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157892 YP_003038373.1 CDS ECBD_4206 NC_012947.1 4432923 4433312 D KEGG: elf:LF82_p590 hypothetical protein, hypothetical protein; hypothetical protein 4432923..4433312 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157893 YP_003038374.1 CDS ECBD_4207 NC_012947.1 4433338 4434561 D PFAM: permease for cytosine/purines uracil thiamine allantoin; KEGG: elf:LF82_p589 permease; permease for cytosine/purines uracil thiamine allantoin 4433338..4434561 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157894 YP_003038375.1 CDS ECBD_4208 NC_012947.1 4434639 4435535 D PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: elf:LF82_p588 hypothetical protein; LysR family transcriptional regulator 4434639..4435535 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157895 YP_003038376.1 CDS ECBD_4209 NC_012947.1 4435629 4437890 R catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine to form methionine; 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase complement(4435629..4437890) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157896 YP_003038377.1 CDS ECBD_4210 NC_012947.1 4438127 4439080 D PFAM: LysR substrate-binding; regulatory protein LysR; KEGG: ssn:SSON_4002 regulator for MetE and MetH; LysR family transcriptional regulator 4438127..4439080 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157897 YP_003038378.1 CDS ECBD_4211 NC_012947.1 4438968 4439867 R TIGRFAM: carboxylate/amino acid/amine transporter; PFAM: protein of unknown function DUF6 transmembrane; KEGG: ssn:SSON_4001 hypothetical protein; carboxylate/amino acid/amine transporter complement(4438968..4439867) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157898 YP_003038379.1 CDS ECBD_4212 NC_012947.1 4439943 4440743 R purine and pyrimidine nucleotides are secondary substrates; yigL expression is regulated by heat shock, osmotic shock and starvation of glucose, phosphate or ammonium; sugar phosphatase complement(4439943..4440743) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157899 YP_003038380.1 CDS ECBD_4213 NC_012947.1 4440751 4441773 R lecithinase B; catalyzes the conversion of 1-lysophosphatidylcholine to glycerophosphocholine; can also hydrolyze 2-acyl glycerophosphoethanolamine and other substrates; lysophospholipase L2 complement(4440751..4441773) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157900 YP_003038381.1 CDS rhtB NC_012947.1 4441884 4442504 D TIGRFAM: homoserine/threonine efflux pump; PFAM: lysine exporter protein (LYSE/YGGA); KEGG: sbc:SbBS512_E4291 homoserine/homoserine lactone efflux protein; homoserine/homoserine lactone efflux protein 4441884..4442504 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157901 YP_003038382.1 CDS ECBD_4215 NC_012947.1 4442566 4443186 R TIGRFAM: homoserine/threonine efflux pump; PFAM: lysine exporter protein (LYSE/YGGA); KEGG: sfv:SFV_3675 threonine efflux system; threonine efflux system complement(4442566..4443186) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157902 YP_003038383.1 CDS ECBD_4216 NC_012947.1 4443250 4445085 R functions in blocking illegitimate recombination, enhancing topoisomerase activity, initiating SOS signaling and clearing blocked replication forks; component of the RecF recombinational pathway; ATP-dependent DNA helicase RecQ complement(4443250..4445085) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157903 YP_003038384.1 CDS ECBD_4217 NC_012947.1 4445204 4446073 R catalyzes the hydrolysis of phosphatidylcholine; phospholipase A complement(4445204..4446073) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157904 YP_003038385.1 CDS ECBD_4218 NC_012947.1 4446238 4446705 D PFAM: thioesterase superfamily protein; KEGG: sbc:SbBS512_E4287 thioesterase family protein; hypothetical protein 4446238..4446705 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157905 YP_003038386.1 CDS ECBD_4219 NC_012947.1 4446757 4447647 D TIGRFAM: RarD protein, DMT superfamily transporter; PFAM: protein of unknown function DUF6 transmembrane; KEGG: sfv:SFV_3679 hypothetical protein; RarD protein, DMT superfamily transporter 4446757..4447647 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157906 YP_003038387.1 CDS ECBD_4220 NC_012947.1 4448136 4448516 D KEGG: sfv:SFV_3681 hypothetical protein; hypothetical protein 4448136..4448516 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157907 YP_003038388.1 CDS ECBD_4222 NC_012947.1 4448886 4449734 D KEGG: elf:LF82_p579 hypothetical protein; hypothetical protein 4448886..4449734 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157909 YP_003038389.1 CDS ECBD_4223 NC_012947.1 4449731 4450222 D PFAM: protein of unknown function DUF943; KEGG: elf:LF82_p578 hypothetical protein; hypothetical protein 4449731..4450222 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158580 YP_003038390.1 CDS ECBD_4224 NC_012947.1 4450264 4451214 R responsible for the influx of magnesium ions; magnesium/nickel/cobalt transporter CorA complement(4450264..4451214) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157989 YP_003038391.1 CDS ECBD_4225 NC_012947.1 4451458 4451571 R KEGG: sbc:SbBS512_E4280 hypothetical protein; hypothetical protein complement(4451458..4451571) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157990 YP_003038392.1 CDS ECBD_4226 NC_012947.1 4451584 4452348 D KEGG: sdy:SDY_3930 predicted periplasmic protein; periplasmic protein 4451584..4452348 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157991 YP_003038393.1 CDS ECBD_4227 NC_012947.1 4452431 4453333 D PFAM: protein of unknown function DUF62; KEGG: sdy:SDY_3931 hypothetical protein; hypothetical protein 4452431..4453333 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157992 YP_003038394.1 CDS uvrD NC_012947.1 4453388 4455550 R unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present; involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair.; DNA-dependent helicase II complement(4453388..4455550) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157993 YP_003038395.1 CDS ECBD_4229 NC_012947.1 4455634 4456350 R YigB; member of the haloacid dehalogenase (HAD)-like hydrolases superfamily of protein; unknown function; flavin mononucleotide phosphatase complement(4455634..4456350) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157994 YP_003038396.1 CDS xerC NC_012947.1 4456350 4457246 R site-specific tyrosine recombinase which cuts and rejoins DNA molecules; binds cooperatively to specific DNA consensus sites; forms a heterotetrameric complex with XerC; XerCD exhibit similar sequences; essential to convert chromosome dimers to monomers during cell division and functions during plasmid segregation; cell division protein FtsK may regulate the XerCD complex; enzyme from Streptococcus group has unusual active site motifs; site-specific tyrosine recombinase XerC complement(4456350..4457246) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157995 YP_003038397.1 CDS ECBD_4231 NC_012947.1 4457243 4457950 R PFAM: protein of unknown function DUF484; KEGG: sfv:SFV_3689 hypothetical protein; hypothetical protein complement(4457243..4457950) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157996 YP_003038398.1 CDS dapF NC_012947.1 4457947 4458771 R involved in lysine biosynthesis; DAP epimerase; produces DL-diaminopimelate from LL-diaminopimelate; diaminopimelate epimerase complement(4457947..4458771) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157997 YP_003038399.1 CDS ECBD_4233 NC_012947.1 4458808 4459011 R KEGG: ssn:SSON_3981 hypothetical protein; hypothetical protein complement(4458808..4459011) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157998 YP_003038400.1 CDS ECBD_4234 NC_012947.1 4459200 4459433 D KEGG: efe:EFER_3695 conserved hypothetical protein; exported protein; hypothetical protein 4459200..4459433 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157999 YP_003038401.1 CDS cyaY NC_012947.1 4459481 4459801 D defects in the mitochondrial frataxin protein cause Friedreich ataxis which is an autosomal recessive neurodegenerative disease; based on phylogenomic distribution this protein may have a role in iron-sulfur cluster protein assembly; frataxin-like protein 4459481..4459801 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158000 YP_003038402.1 CDS cyaA NC_012947.1 4459841 4462387 R catalyzes transfer of adenylyl group of ATP from pyrophosphate to the 3'-hydroxyl group to form cyclic AMP; adenylate cyclase complement(4459841..4462387) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158001 YP_003038403.1 CDS hemC NC_012947.1 4462753 4463715 D transformation of porphobilinogen to hydroxymethylbilane in porphyrin biosynthesis; porphobilinogen deaminase 4462753..4463715 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158002 YP_003038404.1 CDS hemD NC_012947.1 4463712 4464452 D catalyzes the formation of uroporphyrinogen-III from hydroxymethylbilane; functions in tetrapyrrole and heme biosynthesis; uroporphyrinogen-III synthase 4463712..4464452 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158003 YP_003038405.1 CDS ECBD_4239 NC_012947.1 4464474 4465679 D PFAM: protein of unknown function DUF513 hemX; KEGG: ssn:SSON_3975 uroporphyrinogen III C-methyltransferase; uroporphyrinogen III C-methyltransferase 4464474..4465679 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158004 YP_003038406.1 CDS ECBD_4240 NC_012947.1 4465682 4466878 D TIGRFAM: HemY protein; PFAM: HemY domain protein; hypothetical protein; KEGG: sbc:SbBS512_E4261 HemY protein; protoheme IX biogenesis protein 4465682..4466878 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158005 YP_003038407.1 CDS ECBD_4241 NC_012947.1 4467564 4469219 D PFAM: sulfatase; KEGG: elf:LF82_0162 arylsulfatase; sulfatase 4467564..4469219 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158006 YP_003038408.1 CDS ECBD_4242 NC_012947.1 4469377 4470612 R PFAM: radical SAM protein; KEGG: elf:LF82_0163 arylsulfatase-activating protein AslB; radical SAM protein complement(4469377..4470612) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158007 YP_003038409.1 CDS ECBD_4243 NC_012947.1 4471298 4472683 R uncharacterized member of the amino acid-polyamine-organocation (APC) superfamily of amino acid transporters; unknown function; transporter YifK complement(4471298..4472683) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158012 YP_003038410.1 CDS ECBD_4244 NC_012947.1 4472874 4473614 R KEGG: sfv:SFV_3709 UDP-N-acetyl-D-mannosaminuronic acid transferase; TIGRFAM: glycosyl transferase, WecB/TagA/CpsF family; PFAM: glycosyl transferase WecB/TagA/CpsF; UDP-N-acetyl-D-mannosaminuronic acid transferase complement(4472874..4473614) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158013 YP_003038411.1 CDS ECBD_4245 NC_012947.1 4473617 4474969 R enterobacterial common antigen polymerase; common antigen polymerase complement(4473617..4474969) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158014 YP_003038412.1 CDS ECBD_4246 NC_012947.1 4474966 4476045 R catalyzes the synthesis of a lipid-linked intermediate involved in ECA synthesis; 4-alpha-L-fucosyltransferase complement(4474966..4476045) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158015 YP_003038413.1 CDS ECBD_4247 NC_012947.1 4476042 4477292 R PFAM: polysaccharide biosynthesis protein; KEGG: sbc:SbBS512_E4129 polysaccharide biosynthesis protein; polysaccharide biosynthesis protein complement(4476042..4477292) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158016 YP_003038414.1 CDS ECBD_4248 NC_012947.1 4477294 4478424 R catalyzes the formation of dTDP-D-fucosamine from dTDP-4-oxo-6-deoxy-D-glucose in enterobacterial common antigen biosynthesis; TDP-4-oxo-6-deoxy-D-glucose transaminase complement(4477294..4478424) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158017 YP_003038415.1 CDS ECBD_4249 NC_012947.1 4478429 4479103 R TIGRFAM: TDP-D-fucosamine acetyltransferase; PFAM: GCN5-related N-acetyltransferase; KEGG: elf:LF82_1857 lipopolysaccharide biosynthesis protein RffC; TDP-fucosamine acetyltransferase complement(4478429..4479103) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158018 YP_003038416.1 CDS ECBD_4250 NC_012947.1 4479081 4479962 R TIGRFAM: glucose-1-phosphate thymidylyltransferase; PFAM: nucleotidyl transferase; KEGG: sfv:SFV_3715 glucose-1-phosphate thymidylyltransferase; glucose-1-phosphate thymidylyltransferase complement(4479081..4479962) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158019 YP_003038417.1 CDS ECBD_4251 NC_012947.1 4479981 4481048 R TIGRFAM: dTDP-glucose 4,6-dehydratase; PFAM: NAD-dependent epimerase/dehydratase; Male sterility domain; 3-beta hydroxysteroid dehydrogenase/isomerase; polysaccharide biosynthesis protein CapD; short-chain dehydrogenase/reductase SDR; dTDP-4-dehydrorhamnose reductase; KEGG: ssn:SSON_3960 dTDP-glucose 4,6-dehydratase; dTDP-glucose 4,6-dehydratase complement(4479981..4481048) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158020 YP_003038418.1 CDS wecC NC_012947.1 4481048 4482310 R catalyzes the oxidation of UDP-N-acetyl-D-mannosamine to UDP-N-acetylmannosaminuronic acid; UDP-N-acetyl-D-mannosamine dehydrogenase complement(4481048..4482310) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158021 YP_003038419.1 CDS ECBD_4253 NC_012947.1 4482307 4483437 R KEGG: elf:LF82_1859 UDP-N-acetylglucosamine 2-epimerase; TIGRFAM: UDP-N-acetylglucosamine 2-epimerase; PFAM: UDP-N-acetylglucosamine 2-epimerase; UDP-N-acetylglucosamine 2-epimerase complement(4482307..4483437) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158022 YP_003038420.1 CDS ECBD_4254 NC_012947.1 4483493 4484539 R Enterobacterial Common antigen (ECA) polysaccharide chain length modulation protein; lipopolysaccharide biosynthesis protein WzzE complement(4483493..4484539) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158023 YP_003038421.1 CDS ECBD_4255 NC_012947.1 4484551 4485654 R TIGRFAM: undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphatetransferase; PFAM: glycosyl transferase family 4; KEGG: sbc:SbBS512_E4137 undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphatetransferase; UDP-phosphate alpha-N-acetylglucosaminyl 1-phosphatetransferase complement(4484551..4485654) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158024 YP_003038422.1 CDS ECBD_4256 NC_012947.1 4485666 4485884 R KEGG: sbo:SBO_3794 hypothetical protein; hypothetical protein complement(4485666..4485884) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158025 YP_003038423.1 CDS ECBD_4257 NC_012947.1 4485894 4487153 R KEGG: sdy:SDY_3966 transcription termination factor Rho; TIGRFAM: transcription termination factor Rho; PFAM: H+transporting two-sector ATPase alpha/subunit beta central region; ribonuclease B OB region domain; Rho termination factor domain protein; Rho termination factor RNA-binding; SMART: Cold shock protein; ATPase AAA; transcription termination factor Rho complement(4485894..4487153) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158026 YP_003038424.1 CDS ECBD_4258 NC_012947.1 4487238 4487339 R KEGG: sfx:S3904 Rho operon leader peptide; rho operon leader peptide complement(4487238..4487339) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158027 YP_003038425.1 CDS trxA NC_012947.1 4487480 4487809 R TIGRFAM: thioredoxin; PFAM: thioredoxin domain; KEGG: sea:SeAg_B4142 thioredoxin; thioredoxin complement(4487480..4487809) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158028 YP_003038426.1 CDS ECBD_4260 NC_012947.1 4487940 4489205 D enables ATP-dependent unwinding of double stranded RNA as a component of the RNA degradosome, a multi-enzyme complex important in RNA processing and messenger RNA degradation; ATP-dependent RNA helicase RhlB 4487940..4489205 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158029 YP_003038427.1 CDS ECBD_4261 NC_012947.1 4489339 4490823 D catalyzes the conversion of guanosine 5'-triphosphate,3'-diphosphate (pppGpp) to guanosine 5'-diphosphate,3'-diphosphate (ppGpp); pppGpp and ppGpp control the stringent response during amino acid starvation; guanosine pentaphosphate phosphohydrolase 4489339..4490823 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158030 YP_003038428.1 CDS ECBD_4262 NC_012947.1 4490870 4492891 R single-stranded DNA-dependent ATPase; initiates unwinding at a nick in the DNA; involved in DNA replication; ATP-dependent DNA helicase Rep complement(4490870..4492891) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158031 YP_003038429.1 CDS ECBD_4264 NC_012947.1 4493755 4494036 D PFAM: PpiC-type peptidyl-prolyl cis-trans isomerase; KEGG: sdy:SDY_3973 peptidyl-prolyl cis-trans isomerase C; PpiC-type peptidyl-prolyl cis-trans isomerase 4493755..4494036 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158033 YP_003038430.1 CDS ECBD_4265 NC_012947.1 4494123 4495598 R catalyzes the formation of (R)-2,3-dihydroxy-3-methylbutanoate from (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and isoleucine biosynthesis; ketol-acid reductoisomerase complement(4494123..4495598) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158034 YP_003038431.1 CDS ECBD_4266 NC_012947.1 4495748 4496641 D participates in controlling several genes involved in isoleucine and valine biosynthesis; activates the transcription of the ilvC gene in the presence of acetolactate or acetohydroxybutyrate; DNA-binding transcriptional regulator IlvY 4495748..4496641 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158035 YP_003038432.1 CDS ECBD_4267 NC_012947.1 4496693 4498240 R threonine deaminase; threonine dehydratase; in Escherichia coli, IlvA is part of the isoleucine biosynthetic pathway; threonine dehydratase complement(4496693..4498240) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158036 YP_003038433.1 CDS ECBD_4268 NC_012947.1 4498240 4500090 R catalyzes the dehydration of 2,3-dihydroxy-3-methylbutanoate to 3-methyl-2-oxobutanoate in valine and isoleucine biosynthesis; dihydroxy-acid dehydratase complement(4498240..4500090) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158037 YP_003038434.1 CDS ECBD_4269 NC_012947.1 4500155 4501084 R catalyzes the transamination of the branched-chain amino acids to their respective alpha-keto acids; branched-chain amino acid aminotransferase complement(4500155..4501084) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158038 YP_003038435.1 CDS ilvM NC_012947.1 4501104 4501367 R KEGG: sfv:SFV_3733 acetolactate synthase 2 regulatory subunit; acetolactate synthase 2 regulatory subunit complement(4501104..4501367) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158039 YP_003038436.1 CDS ECBD_4271 NC_012947.1 4501364 4503010 R catalyzes the formation of 2-acetolactate from pyruvate; also known as acetolactate synthase large subunit; acetolactate synthase 2 catalytic subunit complement(4501364..4503010) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158040 YP_003038437.1 CDS ECBD_4272 NC_012947.1 4503601 4505121 D among the AAA+ ATPases, the YifB protease family belongs to the Helix 2 insert clade; unknown function; ATP-dependent protease 4503601..4505121 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158041 YP_003038438.1 CDS ECBD_4273 NC_012947.1 4505146 4505484 R PFAM: protein of unknown function DUF413; KEGG: sfv:SFV_3736 hypothetical protein; hypothetical protein complement(4505146..4505484) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158042 YP_003038439.1 CDS ECBD_4274 NC_012947.1 4505603 4506442 D negatively regulates the transcription of the flagellar master operon flhDC by binding to the upstream region of the operon; transcriptional regulator HdfR 4505603..4506442 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158043 YP_003038440.1 CDS ECBD_4275 NC_012947.1 4512236 4512928 D PFAM: GntR family transcriptional regulator; regulatory protein GntR HTH; SMART: regulatory protein GntR HTH; GntR family transcriptional regulator 4512236..4512928 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158050 YP_003038441.1 CDS ECBD_4276 NC_012947.1 4512951 4514378 D TIGRFAM: drug resistance transporter EmrB/QacA subfamily; PFAM: major facilitator superfamily MFS_1; drug resistance transporter EmrB/QacA subfamily 4512951..4514378 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158051 YP_003038442.1 CDS ECBD_4277 NC_012947.1 4514344 4515327 R DNA-binding transcriptional repressor of ribose metabolism; transcriptional repressor RbsR complement(4514344..4515327) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158052 YP_003038443.1 CDS ECBD_4278 NC_012947.1 4515340 4516269 R catalyzes the formation of D-ribose 5-phosphate from ribose; ribokinase complement(4515340..4516269) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158053 YP_003038444.1 CDS ECBD_4279 NC_012947.1 4516395 4517285 R periplasmic substrate-binding component of the ATP-dependent ribose transporter; D-ribose transporter subunit RbsB complement(4516395..4517285) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158054 YP_003038445.1 CDS rbsC NC_012947.1 4517310 4518275 R functions to transport ribose at high affinity; forms a complex with RbsA2C2B; ribose ABC transporter permease complement(4517310..4518275) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158055 YP_003038446.1 CDS ECBD_4281 NC_012947.1 4518280 4519785 R with RbsBCD acts to import ribose into the cell; RbsA contains 2 ATP-binding domain; D-ribose transporter ATP binding protein complement(4518280..4519785) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158056 YP_003038447.1 CDS ECBD_4283 NC_012947.1 4520072 4520593 D KEGG: sfx:S1483 IS103 orf; IS103 orf 4520072..4520593 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158058 YP_003038448.1 CDS ECBD_4284 NC_012947.1 4520590 4521441 D PFAM: Integrase catalytic region; KEGG: sfv:SFV_3530 IS150 ORF2; Integrase catalytic region 4520590..4521441 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158059 YP_003038449.1 CDS trkD NC_012947.1 4521825 4523693 R Responsible for the low-affinity transport of potassium into the cell; involved in potassium ion uptake under hyper-osmotic stress at a low pH; potassium transporter Kup complement(4521825..4523693) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158060 YP_003038450.1 CDS ECBD_4286 NC_012947.1 4523916 4525412 D interacts with LdcI, lysine decarboxylase; may be active in late log/ early stationary phase; regulatory ATPase RavA 4523916..4525412 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158061 YP_003038451.1 CDS yieM NC_012947.1 4525406 4526857 D contains a von Willibrand factor type A domain; stimulates the ATPase activity of RavA; hypothetical protein 4525406..4526857 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158062 YP_003038452.1 CDS ECBD_4288 NC_012947.1 4526862 4527854 R catalyzes the formation of asparagine from aspartate and ammonia; asparagine synthetase AsnA complement(4526862..4527854) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158063 YP_003038453.1 CDS ECBD_4289 NC_012947.1 4528006 4528464 D transcriptional repressor of asnA which codes for aspartate-ammonia ligase; DNA-binding transcriptional regulator AsnC 4528006..4528464 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158064 YP_003038454.1 CDS ECBD_4290 NC_012947.1 4528554 4528997 D An electron-transfer protein; flavodoxin binds one FMN molecule, which serves as a redox-active prosthetic group; flavodoxin 4528554..4528997 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158065 YP_003038455.1 CDS ECBD_4291 NC_012947.1 4529376 4531265 D GidA; glucose-inhibited cell division protein A; involved in the 5-carboxymethylaminomethyl modification (mnm(5)s(2)U) of the wobble uridine base in some tRNAs; tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA 4529376..4531265 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158066 YP_003038456.1 CDS gidB NC_012947.1 4531329 4531952 D glucose-inhibited division protein B; SAM-dependent methyltransferase; methylates the N7 position of guanosine in position 527 of 16S rRNA; 16S rRNA methyltransferase GidB 4531329..4531952 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158067 YP_003038457.1 CDS ECBD_4293 NC_012947.1 4532569 4532949 D Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit I associates with the membrane and may be involved with cation translocation; F0F1 ATP synthase subunit I 4532569..4532949 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158068 YP_003038458.1 CDS ECBD_4294 NC_012947.1 4532958 4533773 D Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit A is part of the membrane proton channel F0; F0F1 ATP synthase subunit A 4532958..4533773 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158069 YP_003038459.1 CDS ECBD_4295 NC_012947.1 4533820 4534059 D Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit C is part of the membrane proton channel F0; F0F1 ATP synthase subunit C 4533820..4534059 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158071 YP_003038460.1 CDS ECBD_4296 NC_012947.1 4534121 4534591 D Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit B is part of the membrane proton channel.; F0F1 ATP synthase subunit B 4534121..4534591 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158072 YP_003038461.1 CDS ECBD_4297 NC_012947.1 4534606 4535139 D Produces ATP from ADP in the presence of a proton gradient across the membrane; the subunit delta is part of the catalytic core of the ATP synthase complex; F0F1 ATP synthase subunit delta 4534606..4535139 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158070 YP_003038462.1 CDS ECBD_4298 NC_012947.1 4535152 4536693 D produces ATP from ADP in the presence of a proton gradient across the membrane; the alpha chain is a catalytic subunit; F0F1 ATP synthase subunit alpha 4535152..4536693 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156664 YP_003038463.1 CDS ECBD_4299 NC_012947.1 4536744 4537607 D Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is a regulatory subunit; F0F1 ATP synthase subunit gamma 4536744..4537607 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156665 YP_003038464.1 CDS ECBD_4300 NC_012947.1 4537634 4539016 D Produces ATP from ADP in the presence of a proton gradient across the membrane. The beta chain is a regulatory subunit; F0F1 ATP synthase subunit beta 4537634..4539016 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156666 YP_003038465.1 CDS atpC NC_012947.1 4539037 4539456 D part of catalytic core of ATP synthase; alpha(3)beta(3)gamma(1)delta(1)epsilon(1); involved in producing ATP from ADP in the presence of the proton motive force across the membrane; F0F1 ATP synthase subunit epsilon 4539037..4539456 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156667 YP_003038466.1 CDS glmU NC_012947.1 4539809 4541179 D forms a homotrimer; catalyzes the acetylation of glucosamine-1-phosphate and uridylation of N-acetylglucosamine-1-phosphate to produce UDP-GlcNAc; function in cell wall synthesis; bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase 4539809..4541179 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156668 YP_003038467.1 CDS ECBD_4303 NC_012947.1 4541341 4543170 D Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source; glucosamine--fructose-6-phosphate aminotransferase 4541341..4543170 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156669 YP_003038468.1 CDS ECBD_4304 NC_012947.1 4543484 4544524 D TIGRFAM: phosphate ABC transporter periplasmic phosphate-binding protein; PFAM: extracellular solute-binding protein family 1; KEGG: sfx:S4045 phosphate transporter subunit; phosphate ABC transporter substrate-binding protein 4543484..4544524 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156670 YP_003038469.1 CDS pstC NC_012947.1 4544610 4545569 D part of the ATP-dependent phosphate uptake system PstABCS responsible for inorganic phosphate (Pi) uptake under Pi starvation conditions; phosphate transporter permease subunit PstC 4544610..4545569 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156671 YP_003038470.1 CDS pstA NC_012947.1 4545569 4546459 D Part of the ABC transporter complex PstABCS responsible for inorganic phosphate (Pi) uptake under Pi starvation conditions; phosphate transporter permease subunit PtsA 4545569..4546459 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156672 YP_003038471.1 CDS pstB NC_012947.1 4546642 4547415 D ATP-binding protein; PstABCS is an ATP dependent phosphate uptake system which is responsible for inorganic phosphate uptake during phosphate starvation; phosphate transporter ATP-binding protein 4546642..4547415 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156673 YP_003038472.1 CDS ECBD_4308 NC_012947.1 4547430 4548155 D regulates several genes involved in high affinity phosphate uptake; under conditions of high phosphate concentrations downregulates the PHO regulon; transcriptional regulator PhoU 4547430..4548155 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156674 YP_003038473.1 CDS ECBD_4309 NC_012947.1 4548441 4549277 D positive regulator of the beta-glucoside operon; transcriptional antiterminator; transcriptional antiterminator BglG 4548441..4549277 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156675 YP_003038474.1 CDS ECBD_4310 NC_012947.1 4549410 4551287 D phosphoenolpyruvate-dependent sugar phosphotransferase system; catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane; IIB is phosphorylated by IIA and then transfers the phosphoryl group to the sugar; IIC forms the translocation channel; PTS system beta-glucoside-specific transporter subunits IIABC 4549410..4551287 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156676 YP_003038475.1 CDS ECBD_4311 NC_012947.1 4551306 4552700 D PFAM: glycoside hydrolase family 1; KEGG: efe:EFER_4019 cryptic phospho-beta-glucosidase B; glycoside hydrolase family 1 4551306..4552700 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156677 YP_003038476.1 CDS ECBD_4312 NC_012947.1 4552786 4554402 D PFAM: porin LamB type; KEGG: ssn:SSON_3915 receptor protein; porin LamB type 4552786..4554402 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156678 YP_003038477.1 CDS ECBD_4313 NC_012947.1 4554429 4555598 D PFAM: glycoside hydrolase family 13 domain protein; esterase; KEGG: elf:LF82_3383 uncharacterized protein yiel; glycoside hydrolase family 13 domain protein 4554429..4555598 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156679 YP_003038478.1 CDS ECBD_4314 NC_012947.1 4555613 4556335 D PFAM: glucosamine/galactosamine-6-phosphate isomerase; KEGG: sfv:SFV_3789 6-phosphogluconolactonase; 6-phosphogluconolactonase 4555613..4556335 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156680 YP_003038479.1 CDS ECBD_4315 NC_012947.1 4556397 4556984 R PFAM: protein of unknown function UPF0167; KEGG: efe:EFER_4015 hypothetical protein; hypothetical protein complement(4556397..4556984) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156681 YP_003038480.1 CDS ECBD_4316 NC_012947.1 4557033 4557506 R might be involved in hypersensitivity to nitrofurzone; inner membrane protein complement(4557033..4557506) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156682 YP_003038481.1 CDS ECBD_4317 NC_012947.1 4557573 4558238 R YieH; catalyzes the dephosphorylation of phosphoenolpyruvate, AMP and p-nitrophenyl phosphate; purine and pyrimidine nucleotides are secondary substrates; member of the haloacid dehalogenase-like hydrolases superfamily; 6-phosphogluconate phosphatase complement(4557573..4558238) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156683 YP_003038482.1 CDS ECBD_4318 NC_012947.1 4558405 4559742 D PFAM: xanthine/uracil/vitamin C permease; KEGG: elf:LF82_3380 inner membrane protein YieG; xanthine/uracil/vitamin C permease 4558405..4559742 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156684 YP_003038483.1 CDS ECBD_4319 NC_012947.1 4559796 4560362 R PFAM: NADPH-dependent FMN reductase; KEGG: sfv:SFV_3798 hypothetical protein; NADPH-dependent FMN reductase complement(4559796..4560362) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156685 YP_003038484.1 CDS ECBD_4320 NC_012947.1 4560384 4561133 R KEGG: sbc:SbBS512_E4210 hypothetical protein; hypothetical protein complement(4560384..4561133) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156686 YP_003038485.1 CDS ECBD_4321 NC_012947.1 4561290 4562249 R Involved in anaerobic NO protection; DNA-binding transcriptional regulator YidZ complement(4561290..4562249) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156687 YP_003038486.1 CDS ECBD_4322 NC_012947.1 4562224 4563399 R Confers resistance to chloramphenicol; multidrug efflux system protein MdtL complement(4562224..4563399) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156688 YP_003038487.1 CDS ECBD_4323 NC_012947.1 4563531 4564778 R tryptophan transporter of low affinity; tryptophan permease TnaB complement(4563531..4564778) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156689 YP_003038488.1 CDS tnaA NC_012947.1 4564869 4566284 R tryptophan indole-lyase; catalyzes the formation of indole and pyruvate from tryptophan; tryptophanase complement(4564869..4566284) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156690 YP_003038489.1 CDS ECBD_4325 NC_012947.1 4566505 4566579 R TIGRFAM: tryptophanase leader peptide; PFAM: tryptophanese operon leader peptide; KEGG: efe:EFER_4509 tryptophanase leader peptide; tryptophanase leader peptide complement(4566505..4566579) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156691 YP_003038490.1 CDS trmE NC_012947.1 4566822 4568186 R in Escherichia coli this protein is involved in the biosynthesis of the hypermodified nucleoside 5-methylaminomethyl-2-thiouridine, which is found in the wobble position of some tRNAs and affects ribosomal frameshifting; shows potassium-dependent dimerization and GTP hydrolysis; also involved in regulation of glutamate-dependent acid resistance and activation of gadE; tRNA modification GTPase TrmE complement(4566822..4568186) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156692 YP_003038491.1 CDS ECBD_4327 NC_012947.1 4568292 4569938 R functions to insert inner membrane proteins into the IM in Escherichia coli; interacts with transmembrane segments; functions in both Sec-dependent and -independent membrane insertion; similar to Oxa1p in mitochondria; inner membrane protein translocase component YidC complement(4568292..4569938) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156693 YP_003038492.1 CDS ECBD_4328 NC_012947.1 4569941 4570198 R PFAM: protein of unknown function DUF37; KEGG: sdy:SDY_4187 hypothetical protein; hypothetical protein complement(4569941..4570198) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156694 YP_003038493.1 CDS rnpA NC_012947.1 4570162 4570488 R protein component of RNaseP which catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'terminus; this enzyme also cleaves other RNA substrates; ribonuclease P complement(4570162..4570488) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156695 YP_003038494.1 CDS rpmH NC_012947.1 4570538 4570678 R in Escherichia coli transcription of this gene is enhanced by polyamines; 50S ribosomal protein L34 complement(4570538..4570678) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156696 ECBD_R0001 tRNA ECBD_R0001 NC_012947.1 69083 69173 R tRNA-Sec complement(69083..69173) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160271 ECBD_R0002 tRNA ECBD_R0002 NC_012947.1 202026 202102 D tRNA-Pro 202026..202102 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157044 ECBD_R0004 tRNA ECBD_R0004 NC_012947.1 485482 485558 D tRNA-Ile 485482..485558 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159464 ECBD_R0005 tRNA ECBD_R0005 NC_012947.1 485601 485676 D tRNA-Ala 485601..485676 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159465 ECBD_R0008 tRNA ECBD_R0008 NC_012947.1 488992 489067 D tRNA-Thr 488992..489067 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159468 ECBD_R0010 tRNA ECBD_R0010 NC_012947.1 583147 583233 D tRNA-Leu 583147..583233 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158747 ECBD_R0011 tRNA ECBD_R0011 NC_012947.1 587016 587092 D tRNA-Met 587016..587092 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158750 ECBD_R0013 tRNA ECBD_R0013 NC_012947.1 692049 692124 R tRNA-Met complement(692049..692124) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158691 ECBD_R0014 tRNA ECBD_R0014 NC_012947.1 795929 796004 R tRNA-Phe complement(795929..796004) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159689 ECBD_R0016 tRNA ECBD_R0016 NC_012947.1 909985 910058 D tRNA-Gly 909985..910058 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159794 ECBD_R0017 tRNA ECBD_R0017 NC_012947.1 953941 954017 R tRNA-Met complement(953941..954017) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156724 ECBD_R0018 tRNA ECBD_R0018 NC_012947.1 954051 954127 R tRNA-Met complement(954051..954127) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156725 ECBD_R0019 tRNA ECBD_R0019 NC_012947.1 954161 954237 R tRNA-Met complement(954161..954237) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156726 ECBD_R0020 tRNA ECBD_R0020 NC_012947.1 1081293 1081385 D tRNA-Ser 1081293..1081385 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156083 ECBD_R0021 tRNA ECBD_R0021 NC_012947.1 1081389 1081465 D tRNA-Arg 1081389..1081465 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156084 ECBD_R0022 tRNA ECBD_R0022 NC_012947.1 1081664 1081740 D tRNA-Arg 1081664..1081740 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156085 ECBD_R0023 tRNA ECBD_R0023 NC_012947.1 1081803 1081879 D tRNA-Arg 1081803..1081879 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156086 ECBD_R0024 tRNA ECBD_R0024 NC_012947.1 1082078 1082154 D tRNA-Arg 1082078..1082154 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156087 ECBD_R0027 tRNA ECBD_R0027 NC_012947.1 1143860 1143935 D tRNA-Glu 1143860..1143935 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159538 ECBD_R0030 tRNA ECBD_R0030 NC_012947.1 1342039 1342114 R tRNA-Lys complement(1342039..1342114) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157420 ECBD_R0031 tRNA ECBD_R0031 NC_012947.1 1342119 1342194 R tRNA-Val complement(1342119..1342194) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157421 ECBD_R0032 tRNA ECBD_R0032 NC_012947.1 1342241 1342316 R tRNA-Val complement(1342241..1342316) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157422 ECBD_R0033 tRNA ECBD_R0033 NC_012947.1 1342361 1342436 R tRNA-Val complement(1342361..1342436) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157423 ECBD_R0034 tRNA ECBD_R0034 NC_012947.1 1345136 1345211 D tRNA-Ala 1345136..1345211 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157913 ECBD_R0035 tRNA ECBD_R0035 NC_012947.1 1345251 1345326 D tRNA-Ala 1345251..1345326 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157914 ECBD_R0036 tRNA ECBD_R0036 NC_012947.1 1385418 1385492 R tRNA-Arg complement(1385418..1385492) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157945 ECBD_R0037 tRNA ECBD_R0037 NC_012947.1 1558672 1558748 R tRNA-Pro complement(1558672..1558748) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160004 ECBD_R0038 tRNA ECBD_R0038 NC_012947.1 1772325 1772400 R tRNA-Asn complement(1772325..1772400) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156481 ECBD_R0039 tRNA ECBD_R0039 NC_012947.1 1774734 1774809 R tRNA-Asn complement(1774734..1774809) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156484 ECBD_R0040 tRNA ECBD_R0040 NC_012947.1 1776558 1776633 D tRNA-Asn 1776558..1776633 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156486 ECBD_R0041 tRNA ECBD_R0041 NC_012947.1 1790037 1790112 R tRNA-Asn complement(1790037..1790112) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156493 ECBD_R0042 tRNA ECBD_R0042 NC_012947.1 1791104 1791193 D tRNA-Ser 1791104..1791193 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156495 ECBD_R0043 tRNA ECBD_R0043 NC_012947.1 1845220 1845295 D tRNA-Gly 1845220..1845295 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157722 ECBD_R0044 tRNA ECBD_R0044 NC_012947.1 1845350 1845423 D tRNA-Cys 1845350..1845423 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157723 ECBD_R0045 tRNA ECBD_R0045 NC_012947.1 1845436 1845522 D tRNA-Leu 1845436..1845522 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157724 ECBD_R0046 tRNA ECBD_R0046 NC_012947.1 2090618 2090694 R tRNA-Val complement(2090618..2090694) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157247 ECBD_R0047 tRNA ECBD_R0047 NC_012947.1 2090699 2090775 R tRNA-Val complement(2090699..2090775) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157248 ECBD_R0048 tRNA ECBD_R0048 NC_012947.1 2506971 2507055 D tRNA-Tyr 2506971..2507055 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158146 ECBD_R0049 tRNA ECBD_R0049 NC_012947.1 2507265 2507349 D tRNA-Tyr 2507265..2507349 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158147 ECBD_R0050 tRNA ECBD_R0050 NC_012947.1 2682167 2682254 D tRNA-Ser 2682167..2682254 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159809 ECBD_R0051 tRNA ECBD_R0051 NC_012947.1 2745584 2745671 D tRNA-Ser 2745584..2745671 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159867 ECBD_R0052 tRNA ECBD_R0052 NC_012947.1 2851357 2851444 D tRNA-Ser 2851357..2851444 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157493 ECBD_R0053 tRNA ECBD_R0053 NC_012947.1 3042170 3042245 R tRNA-Lys complement(3042170..3042245) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156895 ECBD_R0054 tRNA ECBD_R0054 NC_012947.1 3042378 3042453 R tRNA-Lys complement(3042378..3042453) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156896 ECBD_R0055 tRNA ECBD_R0055 NC_012947.1 3042600 3042675 R tRNA-Lys complement(3042600..3042675) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156897 ECBD_R0056 tRNA ECBD_R0056 NC_012947.1 3042679 3042754 R tRNA-Val complement(3042679..3042754) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156898 ECBD_R0057 tRNA ECBD_R0057 NC_012947.1 3042806 3042881 R tRNA-Lys complement(3042806..3042881) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156899 ECBD_R0058 tRNA ECBD_R0058 NC_012947.1 3042884 3042959 R tRNA-Val complement(3042884..3042959) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156900 ECBD_R0059 tRNA ECBD_R0059 NC_012947.1 3043095 3043170 R tRNA-Lys complement(3043095..3043170) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156901 ECBD_R0060 tRNA ECBD_R0060 NC_012947.1 3125378 3125454 D tRNA-Met 3125378..3125454 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159187 ECBD_R0061 tRNA ECBD_R0061 NC_012947.1 3125463 3125547 D tRNA-Leu 3125463..3125547 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159188 ECBD_R0062 tRNA ECBD_R0062 NC_012947.1 3125571 3125645 D tRNA-Gln 3125571..3125645 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159189 ECBD_R0063 tRNA ECBD_R0063 NC_012947.1 3125680 3125754 D tRNA-Gln 3125680..3125754 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159190 ECBD_R0064 tRNA ECBD_R0064 NC_012947.1 3125770 3125846 D tRNA-Met 3125770..3125846 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159191 ECBD_R0065 tRNA ECBD_R0065 NC_012947.1 3125894 3125968 D tRNA-Gln 3125894..3125968 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159192 ECBD_R0066 tRNA ECBD_R0066 NC_012947.1 3126006 3126080 D tRNA-Gln 3126006..3126080 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159193 ECBD_R0067 tRNA ECBD_R0067 NC_012947.1 3248492 3248568 R tRNA-Arg complement(3248492..3248568) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156103 ECBD_R0069 tRNA ECBD_R0069 NC_012947.1 3467341 3467421 R tRNA-OTHER complement(3467341..3467421) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156234 ECBD_R0070 tRNA ECBD_R0070 NC_012947.1 3514943 3515018 R tRNA-Thr complement(3514943..3515018) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160074 ECBD_R0071 tRNA ECBD_R0071 NC_012947.1 3542450 3542526 R tRNA-Asp complement(3542450..3542526) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160108 ECBD_R0072 tRNA ECBD_R0072 NC_012947.1 3550453 3550529 R tRNA-Asp complement(3550453..3550529) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160118 ECBD_R0075 tRNA ECBD_R0075 NC_012947.1 3553970 3554045 R tRNA-Ala complement(3553970..3554045) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160121 ECBD_R0076 tRNA ECBD_R0076 NC_012947.1 3554088 3554164 R tRNA-Ile complement(3554088..3554164) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160122 ECBD_R0078 tRNA ECBD_R0078 NC_012947.1 3817697 3817783 D tRNA-Leu 3817697..3817783 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156995 ECBD_R0079 tRNA ECBD_R0079 NC_012947.1 3817812 3817898 D tRNA-Leu 3817812..3817898 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156996 ECBD_R0080 tRNA ECBD_R0080 NC_012947.1 3817933 3818019 D tRNA-Leu 3817933..3818019 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156997 ECBD_R0081 tRNA ECBD_R0081 NC_012947.1 3934391 3934475 R tRNA-Leu complement(3934391..3934475) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160141 ECBD_R0082 tRNA ECBD_R0082 NC_012947.1 4043295 4043370 R tRNA-Gly complement(4043295..4043370) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158439 ECBD_R0083 tRNA ECBD_R0083 NC_012947.1 4043406 4043481 R tRNA-Gly complement(4043406..4043481) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158440 ECBD_R0084 tRNA ECBD_R0084 NC_012947.1 4043518 4043593 R tRNA-Gly complement(4043518..4043593) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158441 ECBD_R0085 tRNA ECBD_R0085 NC_012947.1 4072761 4072836 D tRNA-Phe 4072761..4072836 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158470 ECBD_R0088 tRNA ECBD_R0088 NC_012947.1 4224597 4224672 R tRNA-Glu complement(4224597..4224672) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156532 ECBD_R0090 tRNA ECBD_R0090 NC_012947.1 4258593 4258668 R tRNA-Thr complement(4258593..4258668) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156562 ECBD_R0091 tRNA ECBD_R0091 NC_012947.1 4258675 4258749 R tRNA-Gly complement(4258675..4258749) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156563 ECBD_R0092 tRNA ECBD_R0092 NC_012947.1 4258866 4258950 R tRNA-Tyr complement(4258866..4258950) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156564 ECBD_R0093 tRNA ECBD_R0093 NC_012947.1 4258959 4259034 R tRNA-Thr complement(4258959..4259034) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156565 ECBD_R0096 tRNA ECBD_R0096 NC_012947.1 4265810 4265885 R tRNA-Glu complement(4265810..4265885) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156571 ECBD_R0100 tRNA ECBD_R0100 NC_012947.1 4399662 4399737 R tRNA-Ala complement(4399662..4399737) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157859 ECBD_R0101 tRNA ECBD_R0101 NC_012947.1 4399780 4399856 R tRNA-Ile complement(4399780..4399856) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157860 ECBD_R0103 tRNA ECBD_R0103 NC_012947.1 4470759 4470835 R tRNA-Pro complement(4470759..4470835) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158008 ECBD_R0104 tRNA ECBD_R0104 NC_012947.1 4470878 4470964 R tRNA-Leu complement(4470878..4470964) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158009 ECBD_R0105 tRNA ECBD_R0105 NC_012947.1 4470985 4471060 R tRNA-His complement(4470985..4471060) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158010 ECBD_R0106 tRNA ECBD_R0106 NC_012947.1 4471119 4471195 R tRNA-Arg complement(4471119..4471195) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158011 ECBD_R0107 tRNA ECBD_R0107 NC_012947.1 4506538 4506613 R tRNA-Trp complement(4506538..4506613) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158044 ECBD_R0108 tRNA ECBD_R0108 NC_012947.1 4506622 4506698 R tRNA-Asp complement(4506622..4506698) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158045 ECBD_R0111 tRNA ECBD_R0111 NC_012947.1 4510053 4510128 R tRNA-Glu complement(4510053..4510128) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158048 ECBD_R0003 rRNA ECBD_R0003 NC_012947.1 483879 485408 D 16S ribosomal RNA 483879..485408 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159463 ECBD_R0006 rRNA ECBD_R0006 NC_012947.1 485862 488767 D 23S ribosomal RNA 485862..488767 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159466 ECBD_R0007 rRNA ECBD_R0007 NC_012947.1 488864 488978 D 5S ribosomal RNA 488864..488978 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159467 ECBD_R0009 rRNA ECBD_R0009 NC_012947.1 489109 489223 D 5S ribosomal RNA 489109..489223 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159469 ECBD_R0026 rRNA ECBD_R0026 NC_012947.1 1142240 1143769 D 16S ribosomal RNA 1142240..1143769 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159537 ECBD_R0028 rRNA ECBD_R0028 NC_012947.1 1144122 1147025 D 23S ribosomal RNA 1144122..1147025 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159539 ECBD_R0029 rRNA ECBD_R0029 NC_012947.1 1147122 1147236 D 5S ribosomal RNA 1147122..1147236 Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8159540 ECBD_R0073 rRNA ECBD_R0073 NC_012947.1 3550583 3550697 R 5S ribosomal RNA complement(3550583..3550697) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160119 ECBD_R0074 rRNA ECBD_R0074 NC_012947.1 3550888 3553793 R 23S ribosomal RNA complement(3550888..3553793) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160120 ECBD_R0077 rRNA ECBD_R0077 NC_012947.1 3554238 3555767 R 16S ribosomal RNA complement(3554238..3555767) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8160123 ECBD_R0086 rRNA ECBD_R0086 NC_012947.1 4221202 4221316 R 5S ribosomal RNA complement(4221202..4221316) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156530 ECBD_R0087 rRNA ECBD_R0087 NC_012947.1 4221507 4224410 R 23S ribosomal RNA complement(4221507..4224410) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156531 ECBD_R0089 rRNA ECBD_R0089 NC_012947.1 4224763 4226292 R 16S ribosomal RNA complement(4224763..4226292) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156533 ECBD_R0094 rRNA ECBD_R0094 NC_012947.1 4262499 4262613 R 5S ribosomal RNA complement(4262499..4262613) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156569 ECBD_R0095 rRNA ECBD_R0095 NC_012947.1 4262710 4265614 R 23S ribosomal RNA complement(4262710..4265614) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156570 ECBD_R0097 rRNA ECBD_R0097 NC_012947.1 4265976 4267505 R 16S ribosomal RNA complement(4265976..4267505) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8156572 ECBD_R0098 rRNA ECBD_R0098 NC_012947.1 4396270 4396384 R 5S ribosomal RNA complement(4396270..4396384) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157857 ECBD_R0099 rRNA ECBD_R0099 NC_012947.1 4396575 4399476 R 23S ribosomal RNA complement(4396575..4399476) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157858 ECBD_R0102 rRNA ECBD_R0102 NC_012947.1 4399930 4401459 R 16S ribosomal RNA complement(4399930..4401459) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8157861 ECBD_R0109 rRNA ECBD_R0109 NC_012947.1 4506752 4506866 R 5S ribosomal RNA complement(4506752..4506866) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158046 ECBD_R0110 rRNA ECBD_R0110 NC_012947.1 4506963 4509866 R 23S ribosomal RNA complement(4506963..4509866) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158047 ECBD_R0112 rRNA ECBD_R0112 NC_012947.1 4510219 4511748 R 16S ribosomal RNA complement(4510219..4511748) Escherichia coli 'BL21-Gold(DE3)pLysS AG' 8158049