-- dump date 20140619_095530 -- class Genbank::Feature -- table feature -- table main -- field 1 id -- field 2 type -- field 3 name -- field 4 contig -- field 5 start_pos -- field 6 end_pos -- field 7 strand -- field 8 description -- field 9 chrom_position -- field 10 organism -- field 11 GeneID -- header -- id type name contig start_pos end_pos strand description chrom_position organism GeneID 177416000001 SEQ_END SEQ_END NC_006570.2 1892775 1892775 DR NC_006570.2; contig end 1892775..1892775 Francisella tularensis subsp. tularensis SCHU S4 YP_169084.1 CDS dnaA NC_006570.2 1 1521 D binds to the dnaA-box as an ATP-bound complex at the origin of replication during the initiation of chromosomal replication; can also affect transcription of multiple genes including itself.; chromosome replication initiator DnaA 1..1521 Francisella tularensis subsp. tularensis SCHU S4 3191523 YP_169085.1 CDS dnaN NC_006570.2 1558 2661 D Similar to DP3B_ECOLI (P00583) DNA polymerase III, beta chain from E. coli (366 aa). FASTA opt: 826 Z-score: 955.1 E(): 2.6e-45 Smith-Waterman score: 826; 35.230 identity in 369 aa overlap; DNA polymerase III subunit beta 1558..2661 Francisella tularensis subsp. tularensis SCHU S4 3191533 YP_169086.1 CDS isftu1 NC_006570.2 3204 3988 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(3204..3662,3662..3988)) Francisella tularensis subsp. tularensis SCHU S4 3192121 YP_169087.1 CDS FTT_0006 NC_006570.2 4661 5869 D Similar to Q9ZE69 Proline/betaine transporter (PROP1) from Rickettsia prowazekii (418 aa). FASTA: opt: 605 Z-score: 665.8 E(): 3.4e-29 Smith-Waterman score: 605; 28.293 identity in 410 aa overlap ORF ftt0006; MFS superfamily proline/ betaine transporter 4661..5869 Francisella tularensis subsp. tularensis SCHU S4 3192082 YP_169088.1 CDS aspS NC_006570.2 6044 7822 D catalyzes a two-step reaction, first charging an aspartate molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; contains discriminating and non-discriminating subtypes; aspartyl-tRNA synthetase 6044..7822 Francisella tularensis subsp. tularensis SCHU S4 3192542 YP_169089.1 CDS FTT_0013c NC_006570.2 11434 12216 R ORF ftt0013c; lipoprotein complement(11434..12216) Francisella tularensis subsp. tularensis SCHU S4 3192126 YP_169090.1 CDS FTT_0014c NC_006570.2 12456 12854 R ORF ftt0014c; hypothetical protein complement(12456..12854) Francisella tularensis subsp. tularensis SCHU S4 3192132 YP_169091.1 CDS purB NC_006570.2 13182 14480 D Catalyzes two discrete reactions in the de novo synthesis of purines: the cleavage of adenylosuccinate and succinylaminoimidazole carboxamide ribotide; adenylosuccinate lyase 13182..14480 Francisella tularensis subsp. tularensis SCHU S4 3191235 YP_169092.1 CDS FTT_0016 NC_006570.2 14514 15092 D ORF ftt0016; hypothetical protein 14514..15092 Francisella tularensis subsp. tularensis SCHU S4 3192139 YP_169093.1 CDS FTT_0017 NC_006570.2 15179 16720 D Similar to Q8PAK3 Toxin secretion ABC transporter ATP-binding protein from Xanthomonas axonopodis (563 aa). FASTA: opt: 454 Z-score: 516.4 E(): 7.1e-21 Smith-Waterman score: 454; 24.395identity in 496 aa overlap ORF ftt0017; hypothetical protein 15179..16720 Francisella tularensis subsp. tularensis SCHU S4 3192135 YP_169094.1 CDS FTT_0018 NC_006570.2 16728 17801 D Similar to Q8PMA5 RND efflux membrane fusion protein from Xanthomonas axonopodis (354 aa). FASTA: opt: 851 Z-score: 971.0 E(): 3.4e-46 Smith-Waterman score: 851; 37.846 identity in 325 aa overlap ORF ftt0018; secretion protein 16728..17801 Francisella tularensis subsp. tularensis SCHU S4 3192140 YP_169095.1 CDS gatC NC_006570.2 17884 18165 D Similar to Q82T57 Glu-tRNAGln amidotransferase C subunit from Nitrosomonas europaea (95 aa). FASTA:i opt: 188 Z-score: 259.9 E(): 1.4e-06 Smith-Waterman score: 188; 31.818 identity in 88 aa overlap; Glu-tRNAGln amidotransferase C subunit 17884..18165 Francisella tularensis subsp. tularensis SCHU S4 3191550 YP_169096.1 CDS gatA NC_006570.2 18174 19619 D allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; aspartyl/glutamyl-tRNA amidotransferase subunit A 18174..19619 Francisella tularensis subsp. tularensis SCHU S4 3191915 YP_169097.1 CDS gatB NC_006570.2 19622 21043 D allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; aspartyl/glutamyl-tRNA amidotransferase subunit B 19622..21043 Francisella tularensis subsp. tularensis SCHU S4 3191603 YP_169098.1 CDS FTT_0022 NC_006570.2 21058 21465 D ORF ftt0022; hypothetical protein 21058..21465 Francisella tularensis subsp. tularensis SCHU S4 3192103 YP_169101.1 CDS FTT_0025c NC_006570.2 23022 24551 R ORF ftt0025c; hypothetical protein complement(23022..24551) Francisella tularensis subsp. tularensis SCHU S4 3192092 YP_169102.1 CDS FTT_0026c NC_006570.2 24636 25826 R Similar to Q9HWP5 Probable MFS transporter from Pseudomonas aeruginosa (402 aa). FASTA: opt: 568 Z-score: 586.0 E(): 9.5e-25 Smith-Waterman score: 568; 26.425 identity in 386 aa overlap ORF ftt0026c; hypothetical protein complement(24636..25826) Francisella tularensis subsp. tularensis SCHU S4 3192094 YP_169103.1 CDS lysA1 NC_006570.2 25842 27095 R Similar to DCDA_BACSU (P23630) Diaminopimelate decarboxylase from Bacillus subtilis (441 aa). FASTA: opt: 437 Z-score: 538.0 E(): 4.5e-22 Smith-Waterman score: 437; 27.295 identity in 414 aa overlap; diaminopimelate decarboxylase complement(25842..27095) Francisella tularensis subsp. tularensis SCHU S4 3192305 YP_169104.1 CDS FTT_0028c NC_006570.2 27088 28350 R Similar to YCEE_ECOLI (P25744) Hypothetical transport protein yce from E. coli (408 aa). FASTA: opt: 364 Z-score: 408.7 E(): 7.1e-15 Smith-Waterman score: 450; 26.582 identity in 395 aa overlap ORF ftt0028c; hypothetical protein complement(27088..28350) Francisella tularensis subsp. tularensis SCHU S4 3192141 YP_169105.1 CDS FTT_0029c NC_006570.2 28357 30276 R Similar to AAO89973 (Q83EA5) FrgA protein from Coxiella burnetii (528 aa). FASTA: opt: 1047 Z-score: 1237.4 E(): 4.9e-61 Smith-Waterman score: 1053; 34.051 identity in 511 aa overlap ORF ftt0029c; hypothetical protein complement(28357..30276) Francisella tularensis subsp. tularensis SCHU S4 3192145 YP_169106.1 CDS fur NC_006570.2 30529 30951 R Similar to FUR_ECOLI (P06975) Ferric uptake regulation protein from E. coli (148 aa). FASTA: opt: 396 Z-score: 515.7 E(): 7.8e-21 Smith-Waterman score: 396; 40.714 identity in 140 aa overlap; ferric uptake regulation protein complement(30529..30951) Francisella tularensis subsp. tularensis SCHU S4 3191536 YP_169107.1 CDS nuoA NC_006570.2 31160 31558 D Similar to Q9A6X0 NADH dehydrogenase I, A subunit from Caulobacter crescentus (125 aa). FASTA: opt: 398 Z-score: 508.1 E(): 2.1e-20 Smith-Waterman score: 398; 46.565 identity in 131 aa overlap; NADH dehydrogenase I subunit A 31160..31558 Francisella tularensis subsp. tularensis SCHU S4 3191755 YP_169108.1 CDS nuoB NC_006570.2 31549 32025 D The point of entry for the majority of electrons that traverse the respiratory chain eventually resulting in the reduction of oxygen; NADH dehydrogenase subunit B 31549..32025 Francisella tularensis subsp. tularensis SCHU S4 3191756 YP_169109.1 CDS nuoC NC_006570.2 32022 32672 D Similar to NUOC_NEIMB (Q9K1C1) NADH-quinone oxidoreductase chain from Neisseria meningitidis (197 aa). FASTA: opt: 516 Z-score: 659.3 E(): 7.8e-29 Smith-Waterman score: 568; 44.286identity in 210 aa overlap; NADH dehydrogenase I 32022..32672 Francisella tularensis subsp. tularensis SCHU S4 3192334 YP_169110.1 CDS nuoD NC_006570.2 32694 33947 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit D 32694..33947 Francisella tularensis subsp. tularensis SCHU S4 3191807 YP_169111.1 CDS nuoE NC_006570.2 33957 34445 D Similar to Q83BQ9 NADH dehydrogenase I, E subunit from Xanthomonas campestris (174 aa). FASTA: opt: 528 Z-score: 689.7 E(): 1.6e-30 Smith-Waterman score: 528; 52.318 identity in 151 aa overlap; NADH dehydrogenase I subunit E 33957..34445 Francisella tularensis subsp. tularensis SCHU S4 3191813 YP_169112.1 CDS nuoF NC_006570.2 34452 35726 D Similar to Q83BR0 NADH dehydrogenase I, F subunit from Coxiella burnetti (422 aa). FASTA: opt: 1851 Z-score: 2306.0 E(): 1.5e-120 Smith-Waterman score: 1851; 61.848 identity in 422 aa overlap; NADH dehydrogenase I subunit F 34452..35726 Francisella tularensis subsp. tularensis SCHU S4 3191815 YP_169113.1 CDS nuoG NC_006570.2 35744 38110 D Catalyzes the transfer of electrons from NADH to ubiquinone; NADH dehydrogenase subunit G 35744..38110 Francisella tularensis subsp. tularensis SCHU S4 3191823 YP_169114.1 CDS nuoH NC_006570.2 38112 39122 D Similar to Q83BR2 NADH dehydrogenase I, H subunit from Coxiella burnetii (340 aa). FASTA: opt: 1510 Z-score: 1695.3 E(): 1.5e-86 Smith-Waterman score: 1510; 60.843 identity in 332 aa overlap; NADH dehydrogenase I subunit H 38112..39122 Francisella tularensis subsp. tularensis SCHU S4 3191826 YP_169115.1 CDS nuoI NC_006570.2 39136 39624 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit I 39136..39624 Francisella tularensis subsp. tularensis SCHU S4 3191832 YP_169116.1 CDS nuoJ NC_006570.2 39629 40234 D Similar to Q9K1B2 NADH dehydrogenase I, J subunit from Neisseria meningitidis (223 aa). FASTA: opt: 387 Z-score: 452.6 E(): 2.6e-17 Smith-Waterman score: 387; 38.191 identity in 199 aa overlap; NADH dehydrogenase I subunit J 39629..40234 Francisella tularensis subsp. tularensis SCHU S4 3191843 YP_169117.1 CDS nuoK NC_006570.2 40212 40544 D Similar to Q9A6Y6 NADH dehydrogenase I, K subunit from Caulobacter crescentus (101 aa). FASTA: opt: 300 Z-score: 405.7 E(): 1e-14 Smith-Waterman score: 300; 43.000 identity in 100 aa overlap; NADH dehydrogenase I subunit K 40212..40544 Francisella tularensis subsp. tularensis SCHU S4 3191853 YP_169118.1 CDS nuoL NC_006570.2 40554 42563 D Similar to Q83BR6 NADH dehydrogenase I, L subunit from Coxiella burnetii (653 aa). FASTA: opt: 1934 Z-score: 2065.6 E(): 3.6e-107 Smith-Waterman score: 2090; 50.760 identity in 658 aa overlap; NADH dehydrogenase I subunit L 40554..42563 Francisella tularensis subsp. tularensis SCHU S4 3192436 YP_169119.1 CDS nuoM NC_006570.2 42589 44178 D Similar to Q83BR7 NADH dehydrogenase I, M subunit from Coxiella burnetii (506 aa). FASTA: opt: 1099 Z-score: 1147.9 E(): 4.8e-56 Smith-Waterman score: 1659; 47.228 identity in 523 aa overlap; NADH dehydrogenase I subunit M 42589..44178 Francisella tularensis subsp. tularensis SCHU S4 3191014 YP_169120.1 CDS nuoN NC_006570.2 44188 45645 D Similar to Q83BR8 NADH dehydrogenase I, N subunit from Coxiella burnetii (482 aa). FASTA: opt: 1231 Z-score: 1222.9 E(): 3.2e-60 Smith-Waterman score: 1231; 39.914 identity in 466 aa overlap; NADH dehydrogenase I subunit N 44188..45645 Francisella tularensis subsp. tularensis SCHU S4 3191022 YP_169121.1 CDS FTT_0045 NC_006570.2 45721 45915 D Similar to Q8DDC7 Conserved hypothetical protein from Vibrio vulnificus (83 aa). FASTA: opt: 165 Z-score: 224.7 E(): 0.00013 Smith-Waterman score: 165; 45.763 identity in 59 aa overlap ORF ftt0045; hypothetical protein 45721..45915 Francisella tularensis subsp. tularensis SCHU S4 3192148 YP_169122.1 CDS hemE NC_006570.2 47462 48496 D catalyzes the formation of coproporphyrinogen from uroporphyrinogen III; uroporphyrinogen decarboxylase 47462..48496 Francisella tularensis subsp. tularensis SCHU S4 3192418 YP_169123.1 CDS FTT_0048 NC_006570.2 48716 49174 D Similar to Q88DV5 Conserved hypothetical protein from Pseudomonas putida (169 aa). FASTA: opt: 402 Z-score: 490.7 E(): 1.7e-19 Smith-Waterman score: 402; 43.243 identity in 148 aa overlap ORF ftt0048; hypothetical protein 48716..49174 Francisella tularensis subsp. tularensis SCHU S4 3192150 YP_169124.1 CDS nusA NC_006570.2 49192 50661 D modifies transcription through interactions with RNA polymerase affecting elongation, readthrough, termination, and antitermination; transcription elongation factor NusA 49192..50661 Francisella tularensis subsp. tularensis SCHU S4 3191035 YP_169125.1 CDS infB NC_006570.2 50700 53240 D Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits during initiation of protein synthesis. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex; translation initiation factor IF-2 50700..53240 Francisella tularensis subsp. tularensis SCHU S4 3192398 YP_169126.1 CDS rbfA NC_006570.2 53243 53674 D Similar to RBFA_ECOLI (P09170) Ribosome-binding factor A from E. coli (132 aa). FASTA: opt: 301 Z-score: 381.1 E(): 2.5e-13 Smith-Waterman score: 301; 41.935 identity in 124 aa overlap; ribosome-binding factor A 53243..53674 Francisella tularensis subsp. tularensis SCHU S4 3191646 YP_169127.1 CDS hisS NC_006570.2 53682 54947 D catalyzes a two-step reaction, first charging a histidine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class II aminoacyl-tRNA synthetase; forms homodimers; some organisms have a paralogous gene, hisZ, that is similar to hisS and produces a protein that performs the first step in histidine biosynthesis along with HisG; histidyl-tRNA synthetase 53682..54947 Francisella tularensis subsp. tularensis SCHU S4 3192430 YP_169128.1 CDS FTT_0053 NC_006570.2 54954 56252 D Similar to AAO90449 (Q83D24) Major facilitator family transporter from Coxiella burnetii (437 aa). FASTA: opt: 908 Z-score: 1023.7 E(): 3.6e-49 Smith-Waterman score: 909; 36.603 identity in 418 aa overlap ORF ftt0053; major facilitator transporter 54954..56252 Francisella tularensis subsp. tularensis SCHU S4 3192152 YP_169129.1 CDS FTT_0054 NC_006570.2 56255 57322 D Similar to YHCM_ECOLI (P46442) Hypothetical protein yhcM from E. coli (375 aa). FASTA: opt: 872 Z-score: 1033.2 E(): 1.2e-49 Smith-Waterman score: 898; 39.833 identity in 359 aa overlap ORF ftt0054; ATPase 56255..57322 Francisella tularensis subsp. tularensis SCHU S4 3190705 YP_169130.1 CDS rluC NC_006570.2 57315 58244 D Similar to RLUC_ECOLI (P23851) Ribosomal large subunit pseudouridine synthase C from E. coli (319 aa). FASTA: opt: 791 Z-score: 972.5 E(): 2.8e-46 Smith-Waterman score: 791; 40.777 identity in 309 aa overlap; ribosomal large subunit pseudouridine synthase C 57315..58244 Francisella tularensis subsp. tularensis SCHU S4 3191616 YP_169131.1 CDS FTT_0056c NC_006570.2 58246 59550 R Similar to Q83D24 (Q83D24) Major facilitator family transporter from Coxiella burnetii (437 aa). FASTA: opt: 1028 Z-score: 1172.6 E(): 2e-57 Smith-Waterman score: 1036; 40.187 identity in 428 aa overlap ORF ftt0056c; major facilitator transporter complement(58246..59550) Francisella tularensis subsp. tularensis SCHU S4 3192153 YP_169132.1 CDS FTT_0057 NC_006570.2 59627 60058 D ORF ftt0057; hypothetical protein 59627..60058 Francisella tularensis subsp. tularensis SCHU S4 3192151 YP_169133.1 CDS atpB NC_006570.2 60111 60902 D Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit A is part of the membrane proton channel F0; ATP synthase F0F1 subunit A 60111..60902 Francisella tularensis subsp. tularensis SCHU S4 3192531 YP_169134.1 CDS atpE NC_006570.2 60953 61258 D produces ATP from ADP in the presence of a proton gradient across the membrane; subunit C is part of the membrane proton channel F0; ATP synthase F0F1 subunit C 60953..61258 Francisella tularensis subsp. tularensis SCHU S4 3192108 YP_169135.1 CDS atpF NC_006570.2 61304 61774 D Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit B is part of the membrane proton channel.; ATP synthase F0F1 subunit B 61304..61774 Francisella tularensis subsp. tularensis SCHU S4 3191918 YP_169136.1 CDS atpH NC_006570.2 61794 62318 D Produces ATP from ADP in the presence of a proton gradient across the membrane; the delta subunit is part of the catalytic core of the ATP synthase complex; ATP synthase F0F1 subunit delta 61794..62318 Francisella tularensis subsp. tularensis SCHU S4 3190962 YP_169137.1 CDS atpA NC_006570.2 62337 63878 D produces ATP from ADP in the presence of a proton gradient across the membrane; the alpha chain is a catalytic subunit; ATP synthase F0F1 subunit alpha 62337..63878 Francisella tularensis subsp. tularensis SCHU S4 3190736 YP_169138.1 CDS atpG NC_006570.2 63893 64789 D produces ATP from ADP in the presence of a proton gradient across the membrane; the gamma chain is a regulatory subunit; ATP synthase F0F1 subunit gamma 63893..64789 Francisella tularensis subsp. tularensis SCHU S4 3192500 YP_169139.1 CDS atpD NC_006570.2 64799 66175 D Produces ATP from ADP in the presence of a proton gradient across the membrane. The beta chain is a regulatory subunit; ATP synthase F0F1 subunit beta 64799..66175 Francisella tularensis subsp. tularensis SCHU S4 3190957 YP_169140.1 CDS atpC NC_006570.2 66188 66625 D produces ATP from ADP in the presence of a proton gradient across the membrane; the epsilon subunit is part of the catalytic core of the ATP synthase complex; ATP synthase F0F1 subunit epsilon 66188..66625 Francisella tularensis subsp. tularensis SCHU S4 3192519 YP_169141.1 CDS FTT_0066 NC_006570.2 66836 69679 D ORF ftt0066; hypothetical protein 66836..69679 Francisella tularensis subsp. tularensis SCHU S4 3192095 YP_169142.1 CDS FTT_0067c NC_006570.2 69696 70025 R Similar to Q8D879 Glutaredoxin-related protein from Vibrio vulnificus (112 aa). FASTA: opt: 485 Z-score: 630.6 E(): 2.8e-27 Smith-Waterman score: 485; 65.306 identity in 98 aa overlap ORF ftt0067c; glutaredoxin-like protein complement(69696..70025) Francisella tularensis subsp. tularensis SCHU S4 3190708 YP_169143.1 CDS sodB NC_006570.2 70161 70739 D Similar to SODF_ECOLI (P09157) Superoxide dismutase [Fe] from E. coli (192 aa). FASTA: opt: 896 Z-score: 1143.2 E(): 8.8e-56 Smith-Waterman score: 896; 66.316 identity in 190 aa overlap; superoxide dismutase 70161..70739 Francisella tularensis subsp. tularensis SCHU S4 3190713 YP_169144.1 CDS FTT_0069c NC_006570.2 70747 70932 R ORF ftt0069c; hypothetical protein complement(70747..70932) Francisella tularensis subsp. tularensis SCHU S4 3190720 YP_169145.1 CDS ampG NC_006570.2 70944 72209 R Similar to AMPG_ECOLI (P36670) AmpG protein from E. coli (491 aa). FASTA: opt: 996 Z-score: 1121.1 E(): 1.5e-54 Smith-Waterman score: 996; 38.614 identity in 404 aa overlap; major facilitator superfamily transporter complement(70944..72209) Francisella tularensis subsp. tularensis SCHU S4 3192356 YP_169146.1 CDS gltA NC_006570.2 72298 73572 R Similar to CISY_PSEAE (P14165) Citrate synthase from Pseudomonas aeruginosa (428 aa). FASTA: opt: 1540 Z-score: 1956.3 E(): 4.5e-101 Smith-Waterman score: 1540; 57.353 identity in 408 aa overlap; citrate synthase complement(72298..73572) Francisella tularensis subsp. tularensis SCHU S4 3192375 YP_169147.1 CDS sdhC NC_006570.2 73815 74288 D Similar to Q884A1 Succinate dehydrogenase, cytochrome b556 subunit from Pseudomonas syringae (124 aa). FASTA: opt: 332 Z-score: 413.6 E(): 3.8e-15 Smith-Waterman score: 332; 39.844identity in 128 aa overlap; succinate dehydrogenase cytochrome b556 73815..74288 Francisella tularensis subsp. tularensis SCHU S4 3191400 YP_169148.1 CDS sdhD NC_006570.2 74264 74632 D Similar to Q88FA6 Succinate dehydrogenase, hydrophobic membrane anchor protein from Pseudomonas putida (122 aa). FASTA: opt: 242 Z-score: 306.8 E(): 3.4e-09 Smith-Waterman score: 242; 34.677 identity in 124 aa overlap; succinate dehydrogenase hydrophobic membrane anchor protein 74264..74632 Francisella tularensis subsp. tularensis SCHU S4 3192101 YP_169149.1 CDS sdhA NC_006570.2 74645 76438 D Similar to Q9I3D5 Succinate dehydrogenase (A subunit) from Pseudomonas aeruginosa (590 aa). FASTA: opt: 2468 Z-score: 2874.7 E(): 3.2e-152 Smith-Waterman score: 2468; 61.139 identity in 597 aa overlap; succinate dehydrogenase catalytic and NAD/flavoprotein subunit 74645..76438 Francisella tularensis subsp. tularensis SCHU S4 3191590 YP_169150.1 CDS sdhB NC_006570.2 76456 77157 D part of four member succinate dehydrogenase enzyme complex that forms a trimeric complex (trimer of tetramers); SdhA/B are the catalytic subcomplex and can exhibit succinate dehydrogenase activity in the absence of SdhC/D which are the membrane components and form cytochrome b556; SdhC binds ubiquinone; oxidizes succinate to fumarate while reducing ubiquinone to ubiquinol; the catalytic subunits are similar to fumarate reductase; succinate dehydrogenase iron-sulfur subunit 76456..77157 Francisella tularensis subsp. tularensis SCHU S4 3191270 YP_169151.1 CDS sucA NC_006570.2 77175 80000 D SucA; E1 component of the oxoglutarate dehydrogenase complex which catalyzes the formation of succinyl-CoA from 2-oxoglutarate; SucA catalyzes the reaction of 2-oxoglutarate with dihydrolipoamide succinyltransferase-lipoate to form dihydrolipoamide succinyltransferase-succinyldihydrolipoate and carbon dioxide; 2-oxoglutarate dehydrogenase E1 77175..80000 Francisella tularensis subsp. tularensis SCHU S4 3190733 YP_169152.1 CDS sucB NC_006570.2 80026 81495 D Similar to Q8EFN9 2-oxoglutarate dehydrogenase,E2 component, dihydrolipoamide succinyltransferase from Shewanella oneidensis (395 aa). FASTA: opt: 1379 Z-score: 1563.0 E(): 3.6e-79 Smith-Waterman score: 1379; 55.949 identity in 395 aa overlap; dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex 80026..81495 Francisella tularensis subsp. tularensis SCHU S4 3190739 YP_169153.1 CDS apt NC_006570.2 81535 82062 D catalyzes a salvage reaction resulting in the formation of AMP which is metabolically less costly than a de novo synthesis; adenine phosphoribosyltransferase 81535..82062 Francisella tularensis subsp. tularensis SCHU S4 3191768 YP_169154.1 CDS glmM NC_006570.2 82073 83404 D catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate; phosphoglucosamine mutase 82073..83404 Francisella tularensis subsp. tularensis SCHU S4 3191618 YP_169155.1 CDS tpiA NC_006570.2 83407 84168 D Reversibly isomerizes the ketone sugar dihydroxyacetone phosphate to the aldehyde sugar glyceraldehyde-3-phosphate; triosephosphate isomerase 83407..84168 Francisella tularensis subsp. tularensis SCHU S4 3191461 YP_169156.1 CDS secG NC_006570.2 84156 84509 D Similar to Q9KU83 Preprotein translocase, SecG subunit from Vibrio cholerae (111 aa). FASTA: opt: 283 Z-score: 369.6 E(): 1.1e-12 Smith-Waterman score: 289; 45.299identity in 117 aa overlap. Together with SecE and SecY forming an integral membrane complex.; preprotein translocase subunit SecG 84156..84509 Francisella tularensis subsp. tularensis SCHU S4 3192467 YP_169157.1 CDS FTT_0083 NC_006570.2 85720 86196 D ORF ftt0083; hypothetical protein 85720..86196 Francisella tularensis subsp. tularensis SCHU S4 3190719 YP_169158.1 CDS hemN NC_006570.2 86193 87335 R Similar to Q87V87 Oxygen-independent coproporphyrinogen III oxidase,,from Pseudomonas syringae (404 aa). FASTA: opt: 1156 Z-score: 1386.6 E(): 2.4e-69 Smith-Waterman score: 1156; 46.791 identity in 374 aa overlap; oxygen-independent coproporphyrinogen III oxidase complement(86193..87335) Francisella tularensis subsp. tularensis SCHU S4 3192423 YP_169159.1 CDS FTT_0085c NC_006570.2 87340 87717 R Similar to YWCD_BACSU P39602 Hypothetical protein ywcD from Bacillus subtilis (127 aa). FASTA: opt: 162 Z-score: 223.9 E(): 0.00013 Smith-Waterman score: 162; 26.667 identity in 120 aa overlap ORF ftt0085c; hypothetical protein complement(87340..87717) Francisella tularensis subsp. tularensis SCHU S4 3190725 YP_169160.1 CDS FTT_0086 NC_006570.2 87787 88665 D Similar to Q9I078 Hypothetical protein PA2765 from Pseudomonas aeruginosa (299 aa). FASTA: opt: 656 Z-score: 793.3 E(): 2.4e-36 Smith-Waterman score: 656; 36.678 identity in 289 aa overlap ORF ftt0086; hypothetical protein 87787..88665 Francisella tularensis subsp. tularensis SCHU S4 3190724 YP_169161.1 CDS acnA NC_006570.2 88770 91583 D Catalyzes the conversion of citrate to isocitrate; aconitate hydratase 88770..91583 Francisella tularensis subsp. tularensis SCHU S4 3192406 YP_169162.1 CDS pilT NC_006570.2 91678 92706 D Similar to Q8EBZ4 Twitching motility protein PilT from Shewanella oneidensis (345 aa). FASTA: opt: 1226 Z-score: 1391.3 E(): 1.3e-69 Smith-Waterman score: 1226; 57.186 identity in 334 aa overlap Required for pili retraction probably by filament disassembly. Homologous to pilB but which has the opposite effect. Unique to type IV pili.; Type IV pili nucleotide-binding protein 91678..92706 Francisella tularensis subsp. tularensis SCHU S4 3190704 YP_169163.1 CDS FTT_0090c NC_006570.2 93768 94214 R Similar to Q87GR3 Hypothetical protein from Vibrio parahaemolyticus (148 aa). FASTA: opt: 160 Z-score: 212.3 E(): 0.00057 Smith-Waterman score: 160; 29.104 identity in 134 aa overlap ORF ftt0090c; hypothetical protein complement(93768..94214) Francisella tularensis subsp. tularensis SCHU S4 3190741 YP_169164.1 CDS appB NC_006570.2 94225 95187 R Similar to Q87H27 Cytochrome BD2, subunit II from Vibrio parahaemolyticus (335 aa). FASTA: opt: 573 Z-score: 669.5 E(): 1.9e-29 Smith-Waterman score: 750; 34.743 identity in 331 aa overlap; cytochrome oxidase bd-II subunit II complement(94225..95187) Francisella tularensis subsp. tularensis SCHU S4 3190938 YP_169165.1 CDS FTT_0093 NC_006570.2 96803 97684 D Similar to Q8FFI5 Hypothetical protein yfcH from E. coli (297 aa). FASTA: opt: 498 Z-score: 611.0 E(): 3.8e-26 Smith-Waterman score: 498; 32.673 identity in 303 aa overlap ORF ftt0093; hypothetical protein 96803..97684 Francisella tularensis subsp. tularensis SCHU S4 3190730 YP_169166.1 CDS qseC NC_006570.2 97681 99108 R Similar to AAO90737 (Q83CA0) Sensor histidine kinase from Coxiella burnetii (478 aa). FASTA: opt: 653 Z-score: 752.8 E(): 4.9e-34 Smith-Waterman score: 677; 29.083 identity in 447 aa overlap Sensory histidine kinase in two-component regulatory system with QseB, regulates flagella and motility by quorum sensing in E. coli, according to Q8X524; sensor histidine kinase complement(97681..99108) Francisella tularensis subsp. tularensis SCHU S4 3191649 YP_169167.1 CDS FTT_0095 NC_006570.2 99224 100180 D ORF ftt0095; hypothetical protein 99224..100180 Francisella tularensis subsp. tularensis SCHU S4 3192116 YP_169168.1 CDS FTT_0096 NC_006570.2 100422 101462 D ORF ftt0096; hypothetical protein 100422..101462 Francisella tularensis subsp. tularensis SCHU S4 3192159 YP_169169.1 CDS FTT_0097 NC_006570.2 101596 102141 D ORF ftt0097; hypothetical protein 101596..102141 Francisella tularensis subsp. tularensis SCHU S4 3192161 YP_169170.1 CDS isftu1 NC_006570.2 102215 102999 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(102215..102673,102673..102999)) Francisella tularensis subsp. tularensis SCHU S4 3190940 YP_169171.1 CDS isftu2 NC_006570.2 103101 103844 D ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 transposase from Francisella tularensis (247 aa).; transposase 103101..103844 Francisella tularensis subsp. tularensis SCHU S4 3190803 YP_169172.1 CDS FTT_0101 NC_006570.2 104514 105527 D Similar to Y402_RICPR (Q9ZDC9) Hypothetical protein RP402 from Rickettsia prowazekii (317 aa). FASTA: opt: 431 Z-score: 518.0 E(): 5.9e-21 Smith-Waterman score: 432; 28.909 identity in 339 aa overlap ORF ftt0101; hypothetical protein 104514..105527 Francisella tularensis subsp. tularensis SCHU S4 3192163 YP_169173.1 CDS FTT_0103c NC_006570.2 106972 107553 R ORF ftt0103c; hypothetical protein complement(106972..107553) Francisella tularensis subsp. tularensis SCHU S4 3192110 YP_169174.1 CDS FTT_0104c NC_006570.2 107798 109075 R Similar to Q83DX6 Major facilitator family transporter from Coxiella burnetii (421 aa). FASTA: opt: 995 Z-score: 1049.4 E(): 1.5e-50 Smith-Waterman score: 995; 39.066identity in 407 aa overlap ORF ftt0104c; major facilitator transporter complement(107798..109075) Francisella tularensis subsp. tularensis SCHU S4 3192109 YP_169175.1 CDS FTT_0105c NC_006570.2 109207 112320 R Similar to AAO90606 (Q83CM1) Transporter,AcrB/AcrD/AcrF family from Coxiella burnetii (1019 aa). FASTA: opt: 2662 Z-score: 2840.6 E(): 2.3e-150 Smith-Waterman score: 3071; 45.171identity in 1025 aa overlap ORF ftt0105c; AcrB/AcrD/AcrF family transporter complement(109207..112320) Francisella tularensis subsp. tularensis SCHU S4 3190746 YP_169176.1 CDS FTT_0106c NC_006570.2 112320 113693 R Similar to Q83CM0 Efflux transporter, RND family, MFP subunit from Coxiella burnetti (380 aa). FASTA: opt: 732 Z-score: 824.0 E(): 5.2e-38 Smith-Waterman score: 781; 34.748identity in 377 aa overlap ORF ftt0106c; RND efflux transporter complement(112320..113693) Francisella tularensis subsp. tularensis SCHU S4 3192105 YP_169177.1 CDS dsbB NC_006570.2 113706 114197 R Similar to DSBB_NEIMB (Q9JYC6) Disulfide bond formation protein B (disuphide oxidoreductase) from Neisseria meningitidis (162 aa). FASTA: opt: 171 Z-score: 223.1 E(): 0.00014 Smith-Waterman score: 171; 27.344 identity in 128 aa overlap; disulfide bond formation protein complement(113706..114197) Francisella tularensis subsp. tularensis SCHU S4 3191543 YP_169178.1 CDS cca NC_006570.2 114237 115319 R catalyzes the addition and repair of the 3'-terminal CCA sequence in tRNA; these proteins belong to the CCA-adding enzyme subfamily 2 which does not have phosphohydrolase activity; tRNA CCA-pyrophosphorylase complement(114237..115319) Francisella tularensis subsp. tularensis SCHU S4 3191540 YP_169179.1 CDS msbA NC_006570.2 115424 117253 D lipid A transport protein, ABC transporter,ATP-binding and membrane protein. Similar to Q47908 ValA from Francisella novicida strain U112 (572 aa). FASTA: opt: 3334 Z-score: 3684.8 E(): 2.4e-197 Smith-Waterman score: 3334; 97.810 identity in 548 aa overlap (54-601:13-559). Similar to MSBA_HAEIN (P44407) Lipid A export ATP-binding protein from Haemophilus influenzae (587 aa). FASTA: opt: 1570 Z-score: 1735.1 E(): 9.4e-89 Smith-Waterman score: 1570; 41.913identity in 575 aa overlap.; lipid A transport protein ABC transporter ATP-binding protein/permease 115424..117253 Francisella tularensis subsp. tularensis SCHU S4 3191929 YP_169180.1 CDS lpxK NC_006570.2 117259 118227 D transfers the gamma-phosphate of ATP to the 4' position of a tetraacyldisaccharide 1-phosphate intermediate to form tetraacyldisaccharide 1,4'-bis-phosphate; tetraacyldisaccharide 4'-kinase 117259..118227 Francisella tularensis subsp. tularensis SCHU S4 3191936 YP_169181.1 CDS polA NC_006570.2 118323 121016 D Similar to AAO91288 (Q83AT5) DNA polymerase I from Coxiella burnetii (895 aa). FASTA: opt: 2961 Z-score: 3329.8 E(): 1.4e-177 Smith-Waterman score: 2961; 52.058 identity in 899 aa overlap; DNA polymerase I 118323..121016 Francisella tularensis subsp. tularensis SCHU S4 3191573 YP_169182.1 CDS FTT_0112 NC_006570.2 121036 121812 D type III; catalyzes the formation of (R)-4'-phosphopantothenate from (R)-pantothenate in coenzyme A biosynthesis; type III pantothenate kinases are not subject to feedback inhibition from coenzyme A and have a high Km for ATP; pantothenate kinase 121036..121812 Francisella tularensis subsp. tularensis SCHU S4 3192123 YP_169183.1 CDS deoB NC_006570.2 121817 123061 D catalyzes the transfer of phosphate between the C1 and C5 carbons of pentose; phosphopentomutase 121817..123061 Francisella tularensis subsp. tularensis SCHU S4 3191513 YP_169184.1 CDS deoC NC_006570.2 123024 123785 D Similar to AAP95774 (Q7VMS9) Deoxyribose-phosphate aldolase from Haemophilus ducreyi (258 aa). FASTA: opt: 632 Z-score: 764.1 E(): 1.1e-34 Smith-Waterman score: 632; 42.857 identity in 245 aa overlap; deoxyribose-phosphate aldolase 123024..123785 Francisella tularensis subsp. tularensis SCHU S4 3191515 YP_169185.1 CDS nupC1 NC_006570.2 123775 124974 D Similar to AAP07626 (Q81I17) Nucleoside permease nupC from Bacillus cereus (392 aa). FASTA: opt: 1236 Z-score: 1430.7 E(): 7.7e-72 Smith-Waterman score: 1236; 49.246 identity in 398 aa overlap Paralog of FTT0116; nucleoside permease NUP family protein 123775..124974 Francisella tularensis subsp. tularensis SCHU S4 3191032 YP_169186.1 CDS nupC1 NC_006570.2 124993 126195 D Similar to (AAP07626) Q81I17 Nucleoside permease nupC from Bacillus cereus (392 aa). FASTA: opt: 1351 Z-score: 1549.9 E(): 1.9e-78 Smith-Waterman score: 1351; 52.381 identity in 399 aa overlap Paralog of FTT0115; nucleoside permease NUP family protein 124993..126195 Francisella tularensis subsp. tularensis SCHU S4 3191029 YP_169187.1 CDS tmk NC_006570.2 126208 126837 D catalyzes the reversible phosphoryl transfer from adenosine triphosphate (ATP) to thymidine monophosphate (dTMP) to form thymidine diphosphate (dTDP); thymidylate kinase 126208..126837 Francisella tularensis subsp. tularensis SCHU S4 3190791 YP_169188.1 CDS prfC NC_006570.2 126899 128476 D stimulates the release of release factors 1 and 2 from the ribosome after hydrolysis of the ester bond in peptidyl-tRNA has occurred; GDP/GTP-binding protein; peptide chain release factor 3 126899..128476 Francisella tularensis subsp. tularensis SCHU S4 3191238 YP_169189.1 CDS FTT_0119 NC_006570.2 128551 129867 D ORF ftt0119; hypothetical protein 128551..129867 Francisella tularensis subsp. tularensis SCHU S4 3190765 YP_169190.1 CDS ftsY NC_006570.2 129864 130895 D Similar to Q9I6C1 Signal recognition particle receptor FtsY from Pseudomonas aeruginosa (455 aa). FASTA: opt: 1262 Z-score: 1403.4 E(): 2.8e-70 Smith-Waterman score: 1262; 61.935identity in 310 aa overlap; signal recognition particle receptor FtsY 129864..130895 Francisella tularensis subsp. tularensis SCHU S4 3191702 YP_169191.1 CDS uvrD NC_006570.2 130986 133208 D Similar to Q88C31 DNA helicase II from Pseudomonas putida (728 aa). FASTA: opt: 1880 Z-score: 2129.9 E(): 9.6e-111 Smith-Waterman score: 2083; 45.867 identity in 750 aa overlap; DNA helicase II 130986..133208 Francisella tularensis subsp. tularensis SCHU S4 3191925 YP_169192.1 CDS oppD NC_006570.2 136633 137601 D Similar to Q8D8A2 Oligopeptide ABC transporter,ATP-binding protein D from Vibrio vulnificus (324 aa). FASTA: opt: 1202 Z-score: 1353.8 E(): 1.6e-67 Smith-Waterman score: 1202; 56.270 identity in 311 aa overlap; oligopeptide ABC transporter ATP-binding protein 136633..137601 Francisella tularensis subsp. tularensis SCHU S4 3191178 YP_169193.1 CDS oppF NC_006570.2 137602 138576 D Similar to OPPF_HAEIN (P45051) Oligopeptide transport ATP-binding oppF from Haemophilus influenzae (332 aa). FASTA: opt: 1227 Z-score: 1398.5 E(): 5.3e-70 Smith-Waterman score: 1227; 56.790 identity in 324 aa overlap; oligopeptide ABC transporter ATP-binding protein 137602..138576 Francisella tularensis subsp. tularensis SCHU S4 3191181 YP_169194.1 CDS FTT_0127c NC_006570.2 138580 139782 R Similar to Q83CF6 Major facilitator family transporter from Coxiella burnetii (443 aa). FASTA: opt: 488 Z-score: 542.0 E(): 2.7e-22 Smith-Waterman score: 490; 27.621identity in 391 aa overlap ORF ftt0127c; major facilitator transporter complement(138580..139782) Francisella tularensis subsp. tularensis SCHU S4 3190764 YP_169195.1 CDS FTT_0128 NC_006570.2 139916 140497 D ORF ftt0128; hypothetical protein 139916..140497 Francisella tularensis subsp. tularensis SCHU S4 3190766 YP_169196.1 CDS FTT_0129 NC_006570.2 140641 141909 D Similar to Q83BM0 (Q83BM0) Major facilitator family transporter from Coxiella burnetii (428 aa). FASTA: opt: 497 Z-score: 533.3 E(): 8.2e-22 Smith-Waterman score: 529; 26.355 identity in 406 aa overlap ORF ftt0129; major facilitator transporter 140641..141909 Francisella tularensis subsp. tularensis SCHU S4 3190769 YP_169197.1 CDS glpK NC_006570.2 141966 143474 D Similar to GLPK_THETN (Q8R8J4) Glycerol kinase from Thermoanaerobacter tengcongensis (497 aa). FASTA: opt: 2233 Z-score: 2691.0 E(): 5.3e-142 Smith-Waterman score: 2233; 66.333 identity in 499 aa overlap. This CDS is disrupted by the insertion of an ISFtu1 element occurring very near the C-terminal end. It is not clear whether this insertion affects the function of the protein; glycerol kinase 141966..143474 Francisella tularensis subsp. tularensis SCHU S4 3192359 YP_169198.1 CDS isftu1 NC_006570.2 143567 144351 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(143567..144025,144025..144351)) Francisella tularensis subsp. tularensis SCHU S4 3190973 YP_169199.1 CDS glpA NC_006570.2 144667 146199 D Similar to O51259 Glycerol-3-phosphate dehydrogenase, anaerobic, from Borrelia burgdorferii (527 aa). FASTA: opt: 1330 Z-score: 1506.9 E(): 4.8e-76 Smith-Waterman score: 1330; 43.333 identity in 510 aa overlap; anaerobic glycerol-3-phosphate dehydrogenase 144667..146199 Francisella tularensis subsp. tularensis SCHU S4 3191077 YP_169200.1 CDS glpF NC_006570.2 146209 146973 D Similar to CAD75967 (Q7UMF0) Probable glycerol uptake facilitator protein from Rhodopirelulla baltica (271 aa). FASTA: opt: 796 Z-score: 888.1 E(): 1.4e-41 Smith-Waterman score: 796; 50.209 identity in 239 aa overlap; glycerol uptake facilitator protein 146209..146973 Francisella tularensis subsp. tularensis SCHU S4 3192242 YP_169201.1 CDS FTT_0134 NC_006570.2 146977 147690 D ORF ftt0134; hypothetical protein 146977..147690 Francisella tularensis subsp. tularensis SCHU S4 3190771 YP_169202.1 CDS FTT_0136 NC_006570.2 148165 149580 D Similar to AAQ00172 (Q7VBG7) DNA helicase related to phage enzyme from Prochlorococcus marinus (479 aa) FASTA: opt: 386 Z-score: 446.1 E(): 5.9e-17 Smith-Waterman score: 386; 26.681 identity in 476 aa overlap ORF ftt0136; helicase 148165..149580 Francisella tularensis subsp. tularensis SCHU S4 3190774 YP_169203.1 CDS tufA NC_006570.2 150045 151229 D EF-Tu; promotes GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis; when the tRNA anticodon matches the mRNA codon, GTP hydrolysis results; the inactive EF-Tu-GDP leaves the ribosome and release of GDP is promoted by elongation factor Ts; many prokaryotes have two copies of the gene encoding EF-Tu; elongation factor Tu 150045..151229 Francisella tularensis subsp. tularensis SCHU S4 3190712 YP_169204.1 CDS secE NC_006570.2 151421 151891 D forms a complex with SecY and SecG; SecYEG forms a protein-conducting channel to which secA binds and translocates targeted polypeptides across the cytoplasmic membrane, a process driven by ATP and a proton-motive force; preprotein translocase subunit SecE 151421..151891 Francisella tularensis subsp. tularensis SCHU S4 3191695 YP_169205.1 CDS nusG NC_006570.2 151907 152440 D Similar to NUSG_VIBVU (Q8DD25) Transcription antitermination protein nusG from Vibrio vulnificus (181 aa). FASTA: opt: 687 Z-score: 868.9 E(): 1.7e-40 Smith-Waterman score: 687; 58.621 identity in 174 aa overlap; transcription antitermination protein nusG 151907..152440 Francisella tularensis subsp. tularensis SCHU S4 3191046 YP_169206.1 CDS rplK NC_006570.2 152509 152943 D binds directly to 23S ribosomal RNA; 50S ribosomal protein L11 152509..152943 Francisella tularensis subsp. tularensis SCHU S4 3192014 YP_169207.1 CDS rplA NC_006570.2 152946 153641 D in Escherichia coli and Methanococcus, this protein autoregulates expression; the binding site in the mRNA mimics the binding site in the 23S rRNA; 50S ribosomal protein L1 152946..153641 Francisella tularensis subsp. tularensis SCHU S4 3192023 YP_169208.1 CDS rplJ NC_006570.2 153811 154329 D binds the two ribosomal protein L7/L12 dimers and anchors them to the large ribosomal subunit; 50S ribosomal protein L10 153811..154329 Francisella tularensis subsp. tularensis SCHU S4 3192015 YP_169209.1 CDS rplL NC_006570.2 154392 154769 D present in two forms; L12 is normal, while L7 is aminoacylated at the N-terminal serine; the only multicopy ribosomal protein; 4:1 ratio of L7/L12 per ribosome; two L12 dimers bind L10; critically important for translation efficiency and fidelity; stimulates GTPase activity of translation factors; 50S ribosomal protein L7/L12 154392..154769 Francisella tularensis subsp. tularensis SCHU S4 3192013 YP_169210.1 CDS rpoB NC_006570.2 154923 158999 D DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates; beta subunit is part of the catalytic core which binds with a sigma factor to produce the holoenzyme; DNA-directed RNA polymerase subunit beta 154923..158999 Francisella tularensis subsp. tularensis SCHU S4 3191982 YP_169211.1 CDS rpoC NC_006570.2 159061 163314 D Similar to Q9AAU1 (Q9AAU1) DNA-directed RNA polymerase, beta subunit from Caulobacter crescentus (1396 aa). FASTA: opt: 5089 Z-score: 5550.7 E(): 0 Smith-Waterman score: 5089; 56.626identity in 1381 aa overlap; DNA-directed RNA polymerase subunit beta 159061..163314 Francisella tularensis subsp. tularensis SCHU S4 3191980 YP_169212.1 CDS FTT_0146 NC_006570.2 163417 163653 D ORF ftt0146; hypothetical protein 163417..163653 Francisella tularensis subsp. tularensis SCHU S4 3190778 YP_169213.1 CDS gcp NC_006570.2 163653 164663 D in most organisms, only the N-terminal domain is present in a single polypeptide; in some archaea this domain is fused to a kinase domain; this gene is essential for growth in Escherichia coli and Bacillus subtilis; the secreted glycoprotease from Pasteurella haemolytica showed specificity for O-sialoglycosylated proteins; the Pyrococcus structure shows DNA-binding properties, iron-binding, ATP-binding, and AP endonuclease activity; DNA-binding/iron metalloprotein/AP endonuclease 163653..164663 Francisella tularensis subsp. tularensis SCHU S4 3192378 YP_169214.1 CDS FTT_0148 NC_006570.2 164777 165943 D Similar to Q27437 Stearoyl-CoA desaturase from Amblyomma americanum (317 aa). FASTA: opt: 653 Z-score: 747.5 E(): 9.6e-34 Smith-Waterman score: 653; 36.897 identity in 290 aa overlap ORF ftt0148; fatty acid desaturase 164777..165943 Francisella tularensis subsp. tularensis SCHU S4 3190777 YP_169215.1 CDS metK NC_006570.2 166155 167315 R catalyzes the formation of S-adenosylmethionine from methionine and ATP; methionine adenosyltransferase; S-adenosylmethionine synthetase complement(166155..167315) Francisella tularensis subsp. tularensis SCHU S4 3190826 YP_169216.1 CDS rpsP NC_006570.2 167609 167857 D binds to lower part of 30S body where it stabilizes two domains; required for efficient assembly of 30S; in Escherichia coli this protein has nuclease activity; 30S ribosomal protein S16 167609..167857 Francisella tularensis subsp. tularensis SCHU S4 3192515 YP_169217.1 CDS rimM NC_006570.2 167900 168409 D Essential for efficient processing of 16S rRNA; 16S rRNA-processing protein RimM 167900..168409 Francisella tularensis subsp. tularensis SCHU S4 3191619 YP_169218.1 CDS trmD NC_006570.2 168806 169570 D methylates guanosine-37 in various tRNAs; uses S-adenosyl-L-methionine to transfer methyl group to tRNA; tRNA (guanine-N(1)-)-methyltransferase 168806..169570 Francisella tularensis subsp. tularensis SCHU S4 3192286 YP_169219.1 CDS rplS NC_006570.2 169570 169917 D this protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site; 50S ribosomal protein L19 169570..169917 Francisella tularensis subsp. tularensis SCHU S4 3192004 YP_169220.1 CDS xerD NC_006570.2 170083 170961 D Similar to Q7ZAJ8 (Q7ZAJ8) Integrase/recombinase XerD from Shewanella oneidensis (300 aa). FASTA: opt: 1039 Z-score: 1285.0 E(): 1.1e-63 Smith-Waterman score: 1039; 52.414 identity in 290 aa overlap; integrase/recombinase 170083..170961 Francisella tularensis subsp. tularensis SCHU S4 3192118 YP_169221.1 CDS FTT_0155 NC_006570.2 171078 171929 D Similar to Q87HU0 oxidoreductase iron/ascorbate from Vibrio parahaemolyticus (279 aa). FASTA: opt: 944 Z-score: 1155.0 E(): 1.9e-56 Smith-Waterman score: 944; 49.110 identity in 281 aa overlap ORF ftt0155; oxidoreductase iron/ascorbate family protein 171078..171929 Francisella tularensis subsp. tularensis SCHU S4 3190781 YP_169222.1 CDS FTT_0156 NC_006570.2 171934 172518 D Similar to Q83EI5 Acid phosphatase, class B from Coxiella burnetti (221 aa). FASTA: opt: 326 Z-score: 430.9 E(): 4.1e-16 Smith-Waterman score: 326; 32.642identity in 193 aa overlap ORF ftt0156; acid phosphatase 171934..172518 Francisella tularensis subsp. tularensis SCHU S4 3190784 YP_169223.1 CDS FTT_0157c NC_006570.2 172515 173399 R Similar to Q8XMR0 Probable lic-1 operon protein from Clostridium perfringens (322 aa). FASTA: opt: 370 Z-score: 410.4 E(): 5.7e-15 Smith-Waterman score: 396; 30.492 identity in 305 aa overlap ORF ftt0157c; licB-like transmembrane protein complement(172515..173399) Francisella tularensis subsp. tularensis SCHU S4 3190874 YP_169224.1 CDS FTT_0158c NC_006570.2 173531 174139 R ORF ftt0158c; hypothetical protein complement(173531..174139) Francisella tularensis subsp. tularensis SCHU S4 3192337 YP_169225.1 CDS FTT_0159c NC_006570.2 174216 175037 R ORF ftt0159c; hypothetical protein complement(174216..175037) Francisella tularensis subsp. tularensis SCHU S4 3190819 YP_169226.1 CDS nudH NC_006570.2 175200 175667 D hydrolyzes diadenosine polyphosphate; dinucleoside polyphosphate hydrolase 175200..175667 Francisella tularensis subsp. tularensis SCHU S4 3191741 YP_169227.1 CDS FTT_0161c NC_006570.2 175677 176303 R Similar to Q8FXE8 PAP2 family protein from Brucella suis (325 aa). FASTA: opt: 314 Z-score: 375.8 E(): 4.8e-13 Smith-Waterman score: 314; 31.915identity in 188 aa overlap ORF ftt0161c; PAP2 family protein complement(175677..176303) Francisella tularensis subsp. tularensis SCHU S4 3192299 YP_169228.1 CDS ampD NC_006570.2 176377 176901 D Similar to Q88PQ9 N-acetyl-anhydromuramyl-L-alanine amidase ampD from Pseudomonas putida (190 aa). FASTA: opt: 623 Z-score: 809.4 E(): 3.4e-37 Smith-Waterman score: 623; 52.000 identity in 175 aa overlap; N-acetyl-anhydromuranmyl-L-alanine amidase 176377..176901 Francisella tularensis subsp. tularensis SCHU S4 3192487 YP_169229.1 CDS parE NC_006570.2 176959 178842 R decatenates newly replicated chromosomal DNA and relaxes positive and negative DNA supercoiling; DNA topoisomerase IV subunit B complement(176959..178842) Francisella tularensis subsp. tularensis SCHU S4 3191416 YP_169230.1 CDS FTT_0164c NC_006570.2 178932 180113 R Similar to Q9KRK6 Multidrug resistance protein from Vibrio cholerae (401 aa). FASTA: opt: 579 Z-score: 614.0 E(): 2.6e-26 Smith-Waterman score: 579; 28.989identity in 376 aa overlap ORF ftt0164c; eflux protein complement(178932..180113) Francisella tularensis subsp. tularensis SCHU S4 3192407 YP_169231.1 CDS FTT_0165c NC_006570.2 180113 181483 R Similar to Q88PJ4 Conserved hypothetical protein from Pseudomonas putida (380 aa). FASTA: opt: 644 Z-score: 755.3 E(): 3.5e-34 Smith-Waterman score: 646; 29.268 identity in 369 aa overlap ORF ftt0165c; lipoprotein complement(180113..181483) Francisella tularensis subsp. tularensis SCHU S4 3192524 YP_169232.1 CDS FTT_0166c NC_006570.2 181486 182142 R Similar to Q8FF57 Hypothetical protein yfgM from E. coli (206 aa). FASTA: opt: 155 Z-score: 188.7 E(): 0.012 Smith-Waterman score: 188; 27.778 identity in 198 aa overlap ORF ftt0166c; hypothetical protein complement(181486..182142) Francisella tularensis subsp. tularensis SCHU S4 3192512 YP_169233.1 CDS hemA NC_006570.2 182323 183573 D catalyzes the formation of glutamate-1-semialdehyde from glutamyl-tRNA(Glu) and NADPH; the second step of the pathway is catalyzed by glutamate-1-semialdehyde aminomutase which results in the formation of 5-aminolevulinic acid; functions in porphyrin (tetrapyrroles) biosynthesis; the crystal structure showed a C-terminal dimerization domain that appears to be absent in Chlamydial proteins; glutamyl-tRNA reductase 182323..183573 Francisella tularensis subsp. tularensis SCHU S4 3192414 YP_169234.1 CDS prfA NC_006570.2 183574 184659 D recognizes the termination signals UAG and UAA during protein translation a specificity which is dependent on amino acid residues residing in loops of the L-shaped tRNA-like molecule of RF1; this protein is similar to release factor 2; peptide chain release factor 1 183574..184659 Francisella tularensis subsp. tularensis SCHU S4 3191241 YP_169235.1 CDS FTT_0169 NC_006570.2 184652 185506 D ORF ftt0169; HemK protein 184652..185506 Francisella tularensis subsp. tularensis SCHU S4 3191135 YP_169236.1 CDS isftu1 NC_006570.2 185991 186775 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(185991..186317,186317..186775) Francisella tularensis subsp. tularensis SCHU S4 3190954 YP_169237.1 CDS FTT_0174 NC_006570.2 188216 188785 D Similar to Q83A22 Hypothetical protein from Coxiella burnetti (192 aa). FASTA: opt: 440 Z-score: 543.8 E(): 2.1e-22 Smith-Waterman score: 440; 37.634 identity in 186 aa overlap ORF ftt0174; hypothetical protein 188216..188785 Francisella tularensis subsp. tularensis SCHU S4 3191454 YP_169238.1 CDS FTT_0175c NC_006570.2 188779 189561 R Similar to AAO80856 (Q836Q5) ABC transporter, ATP-binding protein (269 aa). FASTA: opt: 738 Z-score: 809.5 E(): 3.1e-37 Smith-Waterman score: 738; 43.137 identity in 255 aa overlap missing 90 aa at C-terminus ORF ftt0175c; ABC transporter ATP-binding protein complement(188779..189561) Francisella tularensis subsp. tularensis SCHU S4 3191373 YP_169239.1 CDS FTT_0177c NC_006570.2 191745 192170 R Similar to Q88IP2 Acetyltransferase, GNAT family from Pseudomonas putida (364 aa). FASTA: opt: 250 Z-score: 324.7 E(): 3.1e-10 Smith-Waterman score: 250; 34.559 identity in 136 aa overlap ORF ftt0177c; acetyltransferase complement(191745..192170) Francisella tularensis subsp. tularensis SCHU S4 3191370 YP_169240.1 CDS rimK NC_006570.2 192157 193626 R Similar to Q9KPN3 Ribosomal protein S6 modification protein-related protein from Vibrio cholerae (483 aa). FASTA: opt: 1367 Z-score: 1648.7 E(): 6.1e-84 Smith-Waterman score: 1367; 43.149 identity in 489 aa overlap; 30S ribosomal protein S6 complement(192157..193626) Francisella tularensis subsp. tularensis SCHU S4 3191620 YP_169241.1 CDS FTT_0180 NC_006570.2 195907 196797 D Similar to Q8DDF5 1-acyl-sn-glycerol-3-phosphate acyltransferase from Vibrio vulnificus (271 aa). FASTA: opt: 749 Z-score: 902.5 E(): 2e-42 Smith-Waterman score: 749; 40.370 identity in 270 aa overlap ORF ftt0180; acyltransferase 195907..196797 Francisella tularensis subsp. tularensis SCHU S4 3191367 YP_169242.1 CDS FTT_0181c NC_006570.2 197194 197730 R Similar to Q9X885 small integral membrane protein from Streptomyces coelicolor (167 aa). FASTA: opt: 262 Z-score: 342.1 E(): 3.6e-11 Smith-Waterman score: 262; 30.723 identity in 166 aa overlap ORF ftt0181c; hypothetical protein complement(197194..197730) Francisella tularensis subsp. tularensis SCHU S4 3191366 YP_169243.1 CDS FTT_0182c NC_006570.2 197828 198370 R Similar to Q8D257 YrdC protein from Wigglesworthia glossinidia (194 aa). FASTA: opt: 374 Z-score: 473.0 E(): 1.9e-18 Smith-Waterman score: 374; 39.130 identity in 184 aa overlap ORF ftt0182c; Sua5/YciO/YrdC family protein complement(197828..198370) Francisella tularensis subsp. tularensis SCHU S4 3191365 YP_169244.1 CDS rpsA NC_006570.2 198487 200157 R in Escherichia coli this protein is involved in binding to the leader sequence of mRNAs and is itself bound to the 30S subunit; autoregulates expression via a C-terminal domain; in most gram negative organisms this protein is composed of 6 repeats of the S1 domain while in gram positive there are 4 repeats; the S1 nucleic acid-binding domain is found associated with other proteins; 30S ribosomal protein S1 complement(198487..200157) Francisella tularensis subsp. tularensis SCHU S4 3191976 YP_169245.1 CDS FTT_0184 NC_006570.2 200305 200766 D Similar to Q83DS3 Zinc-binding domain protein from Coxiella burnetii (142 aa). FASTA: opt: 473 Z-score: 642.4 E(): 6.8e-28 Smith-Waterman score: 473; 52.817 identity in 142 aa overlap ORF ftt0184; zinc-binding domain-containing protein 200305..200766 Francisella tularensis subsp. tularensis SCHU S4 3191361 YP_169246.1 CDS ddlB NC_006570.2 200770 201660 D D-alanine--D-alanine ligase; DdlA; DdlB; cytoplasmic; catalyzes the formation of D-alanyl-D-alanine from two D-alanines in peptidoglycan synthesis; there are two forms of this enzyme in Escherichia coli; D-alanyl-alanine synthetase A 200770..201660 Francisella tularensis subsp. tularensis SCHU S4 3191505 YP_169247.1 CDS ftsQ NC_006570.2 201657 202340 D Similar to Q83F15 Cell division protein FtsQ from Coxiella burnetii (243 aa). FASTA: opt: 291 Z-score: 355.4 E(): 6.6e-12 Smith-Waterman score: 291; 23.176 identity in 233 aa overlap; cell division protein FtsQ 201657..202340 Francisella tularensis subsp. tularensis SCHU S4 3192466 YP_169248.1 CDS ftsA NC_006570.2 202442 203704 D Similar to Q87WY9 Cell division protein FtsA from Pseuodomonas syringae (418 aa). FASTA: opt: 992 Z-score: 1194.6 E(): 1.2e-58 Smith-Waterman score: 992; 37.621 identity in 412 aa overlap; cell division protein FtsA 202442..203704 Francisella tularensis subsp. tularensis SCHU S4 3191833 YP_169249.1 CDS ftsZ NC_006570.2 203748 204893 D GTPase; similar structure to tubulin; forms ring-shaped polymers at the site of cell division; other proteins such as FtsA, ZipA, and ZapA, interact with and regulate FtsZ function; cell division protein FtsZ 203748..204893 Francisella tularensis subsp. tularensis SCHU S4 3191126 YP_169250.1 CDS lpxC NC_006570.2 204916 205776 D zinc-dependent; catalyzes the deacetylation of UDP-(3-O-acyl)-N-acetylglucosamine to UDP-3-O-(3-hydroxytetradecanoyl)-glucosamine in the second step of lipid A biosynthesis; UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase 204916..205776 Francisella tularensis subsp. tularensis SCHU S4 3190839 YP_169251.1 CDS dnaX NC_006570.2 205790 207430 R Similar to Q8DB24 DNA polymerase III, gamma/tau subunits from Vibrio vulnificus (734 aa). FASTA: opt: 1193 Z-score: 1334.5 E(): 1.9e-66 Smith-Waterman score: 1193; 44.893 identity in 421 aa overlap; DNA polymerase III subunit gamma and tau complement(205790..207430) Francisella tularensis subsp. tularensis SCHU S4 3191541 YP_169252.1 CDS prfB NC_006570.2 207703 208680 D Similar to RF2_HAEIN (P43918) Peptide chain release factor 2 from Haemophilus influenzae (365 aa). FASTA: opt: 1542 Z-score: 1839.9 E(): 1.4e-94 Smith-Waterman score: 1542; 68.405 identity in 326 aa overlap; peptide chain release factor 2 207703..208680 Francisella tularensis subsp. tularensis SCHU S4 3191240 YP_169253.1 CDS lysU NC_006570.2 208813 210543 D Similar to SYK2_ECOLI (P14825) Lysyl-tRNA synthetase, heat inducible, from E. coli (504 aa). FASTA: opt: 2003 Z-score: 2242.9 E(): 4.9e-117 Smith-Waterman score: 2003; 61.066 identity in 488 aa overlap; lysyl-tRNA synthetase 208813..210543 Francisella tularensis subsp. tularensis SCHU S4 3190947 YP_169254.1 CDS FTT_0193c NC_006570.2 210928 211470 R Similar to Y208_VIBCH (Q9KVD8) Hypothetical protein VC0208 from Vibrio cholerae (162 aa). FASTA: opt: 414 Z-score: 528.5 E(): 1.5e-21 Smith-Waterman score: 414; 41.875 identity in 160 aa overlap ORF ftt0193c; hypothetical protein complement(210928..211470) Francisella tularensis subsp. tularensis SCHU S4 3191362 YP_169255.1 CDS FTT_0194c NC_006570.2 211569 212441 R Similar to Q8X898 Hypothetical 31.1 kDa protein (Hypothetical membrane protein) from E.coli (274 aa). FASTA: opt: 398 Z-score: 431.1 E(): 4e-16 Smith-Waterman score: 398; 26.429 identity in 280 aa overlap ORF ftt0194c; hypothetical protein complement(211569..212441) Francisella tularensis subsp. tularensis SCHU S4 3191359 YP_169256.1 CDS FTT_0195c NC_006570.2 212450 213991 R Similar to Q8IX91 L-glutaminase from Homo sapien (602 aa). FASTA: opt: 1441 Z-score: 1670.0 E(): 3.9e-85 Smith-Waterman score: 1441; 42.887 identity in 478 aa overlap ORF ftt0195c; L-glutamine synthetase complement(212450..213991) Francisella tularensis subsp. tularensis SCHU S4 3191355 YP_169257.1 CDS glnA NC_006570.2 214073 215110 R Similar to AAO90051 (Q83E31) Glutamine synthetase from Coxiella burnetti (359 aa). FASTA: opt: 1369 Z-score: 1705.1 E(): 4.4e-87 Smith-Waterman score: 1369; 59.226 identity in 336 aa overlap; glutamine synthetase complement(214073..215110) Francisella tularensis subsp. tularensis SCHU S4 3191892 YP_169258.1 CDS holA NC_006570.2 215230 216207 R Similar to Q88DM9 DNA polymerase III, delta subunit from Pseudomonas putida (345 aa). FASTA: opt: 380 Z-score: 451.8 E(): 2.8e-17 Smith-Waterman score: 380; 28.707 identity in 317 aa overlap; DNA polymerase III subunit delta complement(215230..216207) Francisella tularensis subsp. tularensis SCHU S4 3192432 YP_169259.1 CDS blc NC_006570.2 216335 216811 D Similar to Q8EGB4 Lipoprotein Blc from Shewenella oneidensis (177 aa). FASTA: opt: 620 Z-score: 813.1 E(): 2.1e-37 Smith-Waterman score: 620; 56.604 identity in 159 aa overlap; outer membrane lipoprotein 216335..216811 Francisella tularensis subsp. tularensis SCHU S4 3192374 YP_169262.1 CDS isftu2 NC_006570.2 218947 219690 R ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 transposase from Francisella tularensis (247 aa).; transposase complement(218947..219690) Francisella tularensis subsp. tularensis SCHU S4 3191805 YP_169263.1 CDS purH NC_006570.2 220941 222488 R involved in de novo purine biosynthesis; bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase complement(220941..222488) Francisella tularensis subsp. tularensis SCHU S4 3191667 YP_169264.1 CDS purA NC_006570.2 222665 223951 D catalyzes the formation of N6-(1,2,-dicarboxyethyl)-AMP from L-aspartate, inosine monophosphate and GTP in AMP biosynthesis; adenylosuccinate synthetase 222665..223951 Francisella tularensis subsp. tularensis SCHU S4 3192003 YP_169265.1 CDS hpt NC_006570.2 223955 224488 D Similar to Q8R7L0 Hypoxanthine-guanine phosphoribosyltransferase from Thermoanaerobacter tengcogenesis (181 aa). FASTA: opt: 509 Z-score: 658.7 E(): 8.5e-29 Smith-Waterman score: 509; 47.337 identity in 169 aa overlap; hypoxanthine-guanine phosphoribosyltransferase 223955..224488 Francisella tularensis subsp. tularensis SCHU S4 3192107 YP_169266.1 CDS FTT_0207c NC_006570.2 225323 226165 R Similar to Q8G5L3 Possible permease of ABC transporter system from Bifidobacterium longum (277 aa). FASTA: opt: 582 Z-score: 622.6 E(): 8.7e-27 Smith-Waterman score: 582; 38.372identity in 258 aa overlap ORF ftt0207c; ABC transporter permease complement(225323..226165) Francisella tularensis subsp. tularensis SCHU S4 3191352 YP_169267.1 CDS FTT_0208c NC_006570.2 226107 226784 R Similar to MNTB_BACSU (O34338) Manganese transport system ATP-binding protein mntB from Bacillus subtilis (250 aa). FASTA: opt: 365 Z-score: 426.7 E(): 7.1e-16 Smith-Waterman score: 365; 30.986 identity in 213 aa overlap ORF ftt0208c; ABC transporter ATP-binding protein complement(226107..226784) Francisella tularensis subsp. tularensis SCHU S4 3191351 YP_169268.1 CDS FTT_0209c NC_006570.2 226786 227703 R Similar to Q88TE8 ABC transporter substrate binding protein from Lactobacillus plantarum (297 aa). FASTA: opt: 555 Z-score: 628.8 E(): 3.9e-27 Smith-Waterman score: 555; 30.935identity in 278 aa overlap ORF ftt0209c; periplasmic solute binding family protein complement(226786..227703) Francisella tularensis subsp. tularensis SCHU S4 3191350 YP_169269.1 CDS FTT_0211c NC_006570.2 229119 229583 R Similar to Q9F6B1 Outer membrane lipoprotein Pcp from Edwardsiella tarda (155 aa). FASTA: opt: 294 Z-score: 329.0 E(): 2e-10 Smith-Waterman score: 294; 35.032 identity in 157 aa overlap ORF ftt0211c; outer membrane lipoprotein complement(229119..229583) Francisella tularensis subsp. tularensis SCHU S4 3191348 YP_169270.1 CDS wrbA NC_006570.2 229594 230190 R Similar to Q9I509 Trp repressor binding protein WrbA from Pseudomonas aeruginosa (198 aa). FASTA: opt: 727 Z-score: 948.0 E(): 6.5e-45 Smith-Waterman score: 727; 54.974 identity in 191 aa overlap; trp repressor binding protein complement(229594..230190) Francisella tularensis subsp. tularensis SCHU S4 3192104 YP_169271.1 CDS rbn NC_006570.2 230197 231384 D Similar to RBN_ECOLI (P32146) tRNA processing ribonuclease BN from E. coli (290 aa). FASTA: opt: 535 Z-score: 578.5 E(): 2.5e-24 Smith-Waterman score: 535; 33.813 identity in 278 aa overlap; tRNA processing ribonuclease BN 230197..231384 Francisella tularensis subsp. tularensis SCHU S4 3191645 YP_169272.1 CDS priA NC_006570.2 231974 234127 D Similar to Q9KNQ3 Primosomal protein N` from Vibrio cholerae (734 aa). FASTA: opt: 1587 Z-score: 1770.8 E(): 9.7e-91 Smith-Waterman score: 1619; 37.823 identity in 735 aa overlap; primosomal protein N' 231974..234127 Francisella tularensis subsp. tularensis SCHU S4 3191237 YP_169273.1 CDS isftu1 NC_006570.2 234257 235041 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(234257..234583,234583..235041) Francisella tularensis subsp. tularensis SCHU S4 3191113 YP_169274.1 CDS FTT_0219c NC_006570.2 235980 236975 R Similar to Q81FW2 Low-affinity inorganic phosphate transport protein from Bacillus cereus (332 aa). FASTA: opt: 932 Z-score: 1065.7 E(): 1.8e-51 Smith-Waterman score: 932; 42.470 identity in 332 aa overlap ORF ftt0219c; phosphate transport protein complement(235980..236975) Francisella tularensis subsp. tularensis SCHU S4 3191345 YP_169275.1 CDS FTT_0220c NC_006570.2 236993 237649 R Similar to Q8XMV6 Hypothetical protein CPE0582 from Clostridium perfringens (210 aa). FASTA: opt: 207 Z-score: 248.6 E(): 5.9e-06 Smith-Waterman score: 207; 24.883 identity in 213 aa overlap ORF ftt0220c; hypothetical protein complement(236993..237649) Francisella tularensis subsp. tularensis SCHU S4 3191343 YP_169276.1 CDS acpA NC_006570.2 237886 239430 D acid phosphatase 237886..239430 Francisella tularensis subsp. tularensis SCHU S4 3192410 YP_169277.1 CDS ybgK NC_006570.2 239437 240213 R Similar to YBGK_HAEIN (P44298) Hypothetical protein HI1730 from Haemophilus influenzae (309 aa). FASTA: opt: 308 Z-score: 386.7 E(): 1.2e-13 Smith-Waterman score: 352; 32.453 identity in 265 aa overlap; hydrolase subunit complement(239437..240213) Francisella tularensis subsp. tularensis SCHU S4 3192142 YP_169278.1 CDS ybgL NC_006570.2 240255 240953 R Similar to Y993_PYRAB (Q9V005) Hypothetical UPF0271 protein PYRAB09930 from Pyrococcus abyssi (255 aa). FASTA: opt: 430 Z-score: 541.0 E(): 3e-22 Smith-Waterman score: 430; 35.294 identity in 238 aa overlap; LamB/YcsF family protein complement(240255..240953) Francisella tularensis subsp. tularensis SCHU S4 3192131 YP_169279.1 CDS isftu1 NC_006570.2 242738 243522 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(242738..243196,243196..243522)) Francisella tularensis subsp. tularensis SCHU S4 3191787 YP_169280.1 CDS FTT_0227c NC_006570.2 243546 243740 R ORF ftt0227c; hypothetical protein complement(243546..243740) Francisella tularensis subsp. tularensis SCHU S4 3191340 YP_169281.1 CDS orn NC_006570.2 243775 244311 R 3'-5' exoribonuclease specific for small oligoribonuclotides; oligoribonuclease complement(243775..244311) Francisella tularensis subsp. tularensis SCHU S4 3191187 YP_169282.1 CDS efp NC_006570.2 244314 244883 R Involved in peptide bond synthesis; alters the affinity of the ribosome for aminoacyl-tRNA; elongation factor P complement(244314..244883) Francisella tularensis subsp. tularensis SCHU S4 3191555 YP_169283.1 CDS FTT_0230c NC_006570.2 244923 245330 R Similar to Q87B50 Type IV pilin from Xylella fastidiosa (142 aa). FASTA: opt: 224 Z-score: 300.3 E(): 7.8e-09 Smith-Waterman score: 224; 32.090 identity in 134 aa overlap The pili fiber building block protein has a highly distinct primary structure; a short positively charged leader sequence, and a highly conserved and highly hydrophobic amino-terminal domain which forms the core of the pilus fiber; A variety of studies suggest that the invariant glycine (G) at -1 is required for prepilin cleavage and assembly and that the glutamate (E) at + 5 is essential for assembly and efficient N-terminal methylation but not cleavage. The region near the carboxy terminus is often hydrophilic and is stabilized by one or two disulfide bridges that may be involved in recognition of receptors. The genes with these characteristics are usually termed pilA, pilE, pilV, pilW, pilX and FimU. (Mattick John S.. 'Type IV Pili and twitching motility'. Annu. Rev. Microbiol. 2002. 56:289-314.) This protein has all the characteristics described above. ORF ftt0230c; Type IV pili fiber building block protein complement(244923..245330) Francisella tularensis subsp. tularensis SCHU S4 3191339 YP_169284.1 CDS htrB NC_006570.2 245343 246266 R Similar to HTRB_ECOLI (P24187) Lipid A biosynthesis lauroyl acyltransferase from E. coli (306 aa). FASTA: opt: 542 Z-score: 697.7 E(): 5.7e-31 Smith-Waterman score: 542; 32.895 identity in 304 aa overlap (8-307:14-306) n.b.Paralog of FTT0232c; acyltransferase complement(245343..246266) Francisella tularensis subsp. tularensis SCHU S4 3191968 YP_169285.1 CDS ddg NC_006570.2 246327 247226 R Similar to Q8ZNA3 (Q8ZNA3) Cold shock-induced palmitoleoyl transferase from Salmonella typhimurium (306 aa). FASTA: opt: 629 Z-score: 782.5 E(): 1.1e-35 Smith-Waterman score: 629; 36.755 identity in 302 aa overlap Paralog of FTT0231c; acyltransferase complement(246327..247226) Francisella tularensis subsp. tularensis SCHU S4 3191506 YP_169286.1 CDS yidC NC_006570.2 247233 248888 R Similar to 60IM_COXBU (P45650) 60 kDa inner-membrane protein homolog from Coxiella burnetti (566 aa). FASTA: opt: 1434 Z-score: 1537.8 E(): 8.4e-78 Smith-Waterman score: 1434; 41.815 identity in 562 aa overlap; inner-membrane protein complement(247233..248888) Francisella tularensis subsp. tularensis SCHU S4 3191276 YP_169287.2 CDS FTT_0234c NC_006570.2 248897 249169 R hypothetical protein complement(248897..249169) Francisella tularensis subsp. tularensis SCHU S4 3191338 YP_169288.1 CDS rnpA NC_006570.2 249121 249522 R Similar to RNPA_VIBCH (Q9KVY2) Ribonuclease P protein component from Vibrio cholerae (118 aa). FASTA: opt: 189 Z-score: 256.8 E(): 2.1e-06 Smith-Waterman score: 189; 35.870 identity in 92 aa overlap; ribonuclease P protein component complement(249121..249522) Francisella tularensis subsp. tularensis SCHU S4 3191068 YP_169289.1 CDS rpmH NC_006570.2 249464 249598 R in Escherichia coli transcription of this gene is enhanced by polyamines; 50S ribosomal protein L34 complement(249464..249598) Francisella tularensis subsp. tularensis SCHU S4 3191988 YP_169290.1 CDS FTT_0237c NC_006570.2 249690 250148 R ORF ftt0237c; hypothetical protein complement(249690..250148) Francisella tularensis subsp. tularensis SCHU S4 3191337 YP_169291.1 CDS aroE1 NC_006570.2 250275 251048 D Similar to AROE_VIBVU (Q8DDD4) Shikimate 5-dehydrogenase from Vibrio vulnificus (277 aa). FASTA: opt: 567 Z-score: 672.1 E(): 1.5e-29 Smith-Waterman score: 567; 37.984 identity in 258 aa overlap; shikimate 5-dehydrogenase 250275..251048 Francisella tularensis subsp. tularensis SCHU S4 3190948 YP_169292.1 CDS murC NC_006570.2 251115 252470 D Similar to Q9F1M9 MurC from Shewanella violacae (494 aa). FASTA: opt: 1010 Z-score: 1212.3 E(): 1.2e-59 Smith-Waterman score: 1010; 36.761 identity in 457 aa overlap; UDP-N-acetylmuramate--L-alanine ligase 251115..252470 Francisella tularensis subsp. tularensis SCHU S4 3191258 YP_169293.1 CDS FTT_0240 NC_006570.2 252463 253335 D Similar to Q87SH3 Conserved hypothetical protein from Vibrio parahaemolyticus (287 aa). FASTA: opt: 744 Z-score: 882.7 E(): 2.8e-41 Smith-Waterman score: 744; 46.996 identity in 283 aa overlap ORF ftt0240; tetrapyrrole methyltransferase family protein 252463..253335 Francisella tularensis subsp. tularensis SCHU S4 3191336 YP_169294.1 CDS FTT_0241c NC_006570.2 253346 253816 R ORF ftt0241c; hypothetical protein complement(253346..253816) Francisella tularensis subsp. tularensis SCHU S4 3191333 YP_169295.1 CDS FTT_0242 NC_006570.2 254156 254719 D Similar to Q83DS9 Hypothetical protein CBU0616 from Coxiella burnetii (196 aa). FASTA: opt: 376 Z-score: 484.1 E(): 4.5e-19 Smith-Waterman score: 376; 37.634 identity in 186 aa overlap ORF ftt0242; hypothetical protein 254156..254719 Francisella tularensis subsp. tularensis SCHU S4 3191334 YP_169296.1 CDS FTT_0243 NC_006570.2 254792 255571 D weak similarity to M. musculus protein,1700056O17 Rik protein (233 aa) ORF ftt0243; hypothetical protein 254792..255571 Francisella tularensis subsp. tularensis SCHU S4 3191331 YP_169297.1 CDS FTT_0244 NC_006570.2 255555 258296 D Similar to Q8R5Z3 DNA/RNA helicase (DEAD/DEAH box family) from Fusobacterium nucleatum (942 aa). FASTA: opt: 2318 Z-score: 2654.5 E(): 5.8e-140 Smith-Waterman score: 2473; 45.087 identity in 916 aa overlap ORF ftt0244; DNA/RNA helicase 255555..258296 Francisella tularensis subsp. tularensis SCHU S4 3191332 YP_169298.1 CDS usp NC_006570.2 258372 259208 D Similar to Q8PWX4 Universal stress protein from Methanosarcina maizae (323 aa). FASTA: opt: 347 Z-score: 418.2 E(): 2.1e-15 Smith-Waterman score: 347; 31.849 identity in 292 aa overlap; universal stress protein 258372..259208 Francisella tularensis subsp. tularensis SCHU S4 3191912 YP_169299.1 CDS isftu1 NC_006570.2 259568 260352 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(259568..259894,259894..260352) Francisella tularensis subsp. tularensis SCHU S4 3190734 YP_169300.1 CDS FTT_0248 NC_006570.2 260522 260893 D ORF ftt0248; hypothetical protein 260522..260893 Francisella tularensis subsp. tularensis SCHU S4 3191328 YP_169301.1 CDS feoB NC_006570.2 260994 263237 D Similar to Q8ZJH5 Ferrous iron transport protein B from Yersinia pestis (771 aa). FASTA: opt: 1928 Z-score: 2034.7 E(): 1.9e-105 Smith-Waterman score: 2307; 45.396 identity in 771 aa overlap; ferrous iron transport protein 260994..263237 Francisella tularensis subsp. tularensis SCHU S4 3191885 YP_169302.1 CDS ppdK NC_006570.2 264049 266697 D catalyzes the formation of phosphoenolpyruvate from pyruvate; pyruvate phosphate dikinase 264049..266697 Francisella tularensis subsp. tularensis SCHU S4 3191412 YP_169303.1 CDS ilvE NC_006570.2 267097 267984 D Similar to Q8Y1X0 Probable branched-chain amino acid aminotransferase protein from Ralstonia solanacearum (309 aa). FASTA: opt: 706 Z-score: 903.1 E(): 2.1e-42 Smith-Waterman score: 706; 39.931 identity in 288 aa overlap; branched-chain amino acid aminotransferase 267097..267984 Francisella tularensis subsp. tularensis SCHU S4 3191569 YP_169304.1 CDS leuA NC_006570.2 267995 269368 D Similar to Q9C5X5 2-isopropylmalate synthase from Arabidopsis thaliana (503 aa). FASTA: opt: 493 Z-score: 601.2 E(): 1.4e-25 Smith-Waterman score: 635; 31.373 identity in 408 aa overlap. This CDS is disrupted by the insertion of an ISFtu1 element occurring very near the C-terminal end. It is not clear whether this insertion affects the function of the protein; 2-isopropylmalate synthase 267995..269368 Francisella tularensis subsp. tularensis SCHU S4 3190775 YP_169305.1 CDS isftu1 NC_006570.2 269465 270249 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(269465..269923,269923..270249)) Francisella tularensis subsp. tularensis SCHU S4 3191118 YP_169308.1 CDS FTT_0256c NC_006570.2 272034 273002 R Similar to Q87T79 lipopolysaccharide A protein from Vibrio parahaemolyticus (311 aa). FASTA: opt: 835 Z-score: 996.3 E(): 1.3e-47 Smith-Waterman score: 910; 43.671 identity in 316 aa overlap ORF ftt0256c; lipopolysaccharide protein complement(272034..273002) Francisella tularensis subsp. tularensis SCHU S4 3191322 YP_169309.1 CDS FTT_0257 NC_006570.2 273191 275047 D Similar to Q8YNW1 Ribonuclease II family protein from Anabaena sp. (686 aa). FASTA: opt: 557 Z-score: 649.1 E(): 2.9e-28 Smith-Waterman score: 763; 28.290 identity in 661 aa overlap ORF ftt0257; ribonuclease II family protein 273191..275047 Francisella tularensis subsp. tularensis SCHU S4 3191323 YP_169310.1 CDS FTT_0258 NC_006570.2 275378 276046 D Similar to Q83AC9 Carboxylesterase/phospholipase family protein from Coxiella burnetii (200 aa). FASTA: opt: 599 Z-score: 736.0 E(): 4.2e-33 Smith-Waterman score: 599; 45.813 identity in 203 aa overlap ORF ftt0258; carboxylesterase/phospholipase family protein 275378..276046 Francisella tularensis subsp. tularensis SCHU S4 3191320 YP_169311.1 CDS hemC NC_006570.2 276043 276945 D transformation of porphobilinogen to hydroxymethylbilane in porphyrin biosynthesis; porphobilinogen deaminase 276043..276945 Francisella tularensis subsp. tularensis SCHU S4 3192416 YP_169312.1 CDS crcB NC_006570.2 276946 277338 D may be involved in chromosome condensation; overexpression in Escherichia coli protects against decondensation by camphor; overexpressing the protein results in an increase in supercoiling; camphor resistance protein CrcB 276946..277338 Francisella tularensis subsp. tularensis SCHU S4 3191891 YP_169313.1 CDS FTT_0261 NC_006570.2 277335 277742 D The predicted signal peptide are in conflict with the membrane lipoprotein lipid attachment site ORF ftt0261; hypothetical protein 277335..277742 Francisella tularensis subsp. tularensis SCHU S4 3191319 YP_169314.1 CDS FTT_0262 NC_006570.2 277882 278019 D The predicted signal peptide and the membrane lipoprotein lipid attachment site are in conflict with each other ORF ftt0262; lipoprotein 277882..278019 Francisella tularensis subsp. tularensis SCHU S4 3191318 YP_169316.1 CDS FTT_0264c NC_006570.2 278449 278622 R Similar to Q8Y1X1 Hypothetical protein RSc0566 from Ralstonia solancearum (65 aa). FASTA: opt: 160 Z-score: 236.6 E(): 2.5e-05 Smith-Waterman score: 160; 50.000 identity in 36 aa overlap ORF ftt0264c; hypothetical protein complement(278449..278622) Francisella tularensis subsp. tularensis SCHU S4 3191308 YP_169317.1 CDS FTT_0265 NC_006570.2 278751 280550 D Similar to Q83AV7 ABC transporter,permease protein from Coxiella burnetii (581 aa). FASTA: opt: 976 Z-score: 1115.9 E(): 2.9e-54 Smith-Waterman score: 1738; 45.318 identity in 598 aa overlap ORF ftt0265; ABC transporter permease 278751..280550 Francisella tularensis subsp. tularensis SCHU S4 3191316 YP_169318.1 CDS FTT_0266 NC_006570.2 280569 281885 D Similar to Q83AV8 ABC transporter, ATP-binding protein from Coxiella burnetii (432 aa). FASTA: opt: 1556 Z-score: 1713.5 E(): 1.5e-87 Smith-Waterman score: 1556; 54.673 identity in 428 aa overlap ORF ftt0266; ABC transporter ATP-binding protein 280569..281885 Francisella tularensis subsp. tularensis SCHU S4 3191315 YP_169319.1 CDS FTT_0267 NC_006570.2 282010 283128 D ORF ftt0267; hypothetical protein 282010..283128 Francisella tularensis subsp. tularensis SCHU S4 3191314 YP_169320.1 CDS FTT_0268 NC_006570.2 283222 285108 D Similar to Q88MV1 Sodium/hydrogen exchanger family protein from Pseudomonas putida (601 aa). FASTA: opt: 1318 Z-score: 1419.4 E(): 3.6e-71 Smith-Waterman score: 1318; 37.993 identity in 608 aa overlap ORF ftt0268; sodium/hydrogen exchanger (antiporter) family protein 283222..285108 Francisella tularensis subsp. tularensis SCHU S4 3191312 YP_169321.1 CDS FTT_0269 NC_006570.2 285093 285632 D Similar to Q8EKP8 Carbonic anhydrase,family 3 from Shewanella oneidensis (182 aa). FASTA: opt: 639 Z-score: 840.8 E(): 6.1e-39 Smith-Waterman score: 639; 50.000 identity in 178 aa overlap ORF ftt0269; carbonic anhydrase 285093..285632 Francisella tularensis subsp. tularensis SCHU S4 3191311 YP_169322.1 CDS lolB NC_006570.2 285583 286218 D Similar to Q83AQ2 Outer membrane lipoprotein LolB, from Coxiella burnetii (210 aa). FASTA: opt: 395 Z-score: 508.7 E(): 1.9e-20 Smith-Waterman score: 395; 33.668 identity in 199 aa overlap; lipoprotein releasing system outer membrane lipoprotein 285583..286218 Francisella tularensis subsp. tularensis SCHU S4 3191459 YP_169323.1 CDS ipk NC_006570.2 286206 287033 D An essential enzyme in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis; 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase 286206..287033 Francisella tularensis subsp. tularensis SCHU S4 3190842 YP_169324.1 CDS FTT_0272 NC_006570.2 287222 287542 D ORF ftt0272; hypothetical protein 287222..287542 Francisella tularensis subsp. tularensis SCHU S4 3191309 YP_169325.1 CDS isftu1 NC_006570.2 287566 288350 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(287566..287892,287892..288350) Francisella tularensis subsp. tularensis SCHU S4 3191277 YP_169327.1 CDS FTT_0277c NC_006570.2 290427 290525 R Similar to Q937K9 YbgT protein from Erwinia chrysanthemi (38 aa). FASTA: opt: 81 Z-score: 153.9 E(): 1 Smith-Waterman score: 81; 42.857 identity in 28 aa overlap ORF ftt0277c; hypothetical protein complement(290427..290525) Francisella tularensis subsp. tularensis SCHU S4 3191304 YP_169328.1 CDS cydB NC_006570.2 290541 291734 R Similar to AAO69749 (Q8XGM0) Cytochrome d ubiquinol oxidase subunit II from Salmonella typhi (379 aa). FASTA: opt: 645 Z-score: 711.2 E(): 1e-31 Smith-Waterman score: 1022; 40.506identity in 395 aa overlap; cytochrome d terminal oxidase polypeptide subunit II complement(290541..291734) Francisella tularensis subsp. tularensis SCHU S4 3191907 YP_169329.1 CDS cydA NC_006570.2 291745 293523 R Similar to AAP96534 (Q7VKT6) Cytochrome D ubiquinol oxidase, subunit I from Haemophilus ducreyi (516 aa). FASTA: opt: 1311 Z-score: 1457.7 E(): 2.7e-73 Smith-Waterman score: 1688; 47.478identity in 575 aa overlap Schu S4 seems to have extra sequence in between 340-420 aa; cytochrome d terminal oxidase polypeptide subunit I complement(291745..293523) Francisella tularensis subsp. tularensis SCHU S4 3191902 YP_169330.1 CDS yajR NC_006570.2 293952 295319 R Similar to AAO89830 (Q83EP3) Major facilitator family transporter from Coxiella burnetii (458 aa). FASTA: opt: 1379 Z-score: 1410.6 E(): 1.1e-70 Smith-Waterman score: 1379; 46.102 identity in 449 aa overlap; major facilitator transporter complement(293952..295319) Francisella tularensis subsp. tularensis SCHU S4 3192130 YP_169331.1 CDS cyoA NC_006570.2 295680 296582 D Similar to Q87DQ7 Cytochrome O ubiquinol oxidase subunit II from Xylella fastidiosa (319 aa). FASTA: opt: 956 Z-score: 1145.6 E(): 6.4e-56 Smith-Waterman score: 956; 49.477 identity in 287 aa overlap; cytochrome O ubiquinol oxidase subunit II 295680..296582 Francisella tularensis subsp. tularensis SCHU S4 3191910 YP_169332.1 CDS cyoB NC_006570.2 296621 298651 D Similar to AAO70008 (Q8XG02) Cytochrome o ubiquinol oxidase subunit I from Salmonella typhi (663 aa). FASTA: opt: 3045 Z-score: 3221.2 E(): 1.6e-171 Smith-Waterman score: 3045; 64.361identity in 665 aa overlap; cytochrome O ubiquinol oxidase subunit I 296621..298651 Francisella tularensis subsp. tularensis SCHU S4 3191917 YP_169333.1 CDS cyoC NC_006570.2 298648 299250 D Similar to Q87IH7 Cytochrome o ubiquinol oxidase, subunit III from Vibrio parahaemolyticus (200 aa). FASTA: opt: 768 Z-score: 931.9 E(): 5.1e-44 Smith-Waterman score: 768; 55.276identity in 199 aa overlap; cytochrome O ubiquinol oxidase subunit III 298648..299250 Francisella tularensis subsp. tularensis SCHU S4 3191921 YP_169334.1 CDS cyoD NC_006570.2 299252 299584 D Similar to Q8ZC55 Cytochrome O ubiquinol oxidase subunit IV from Yersinia pestis (110 aa). FASTA: opt: 325 Z-score: 419.7 E(): 1.7e-15 Smith-Waterman score: 325; 50.505 identity in 99 aa overlap; cytochrome O ubiquinol oxidase subunit IV 299252..299584 Francisella tularensis subsp. tularensis SCHU S4 3191924 YP_169335.1 CDS cyoE NC_006570.2 299615 300463 D converts protoheme IX and farnesyl diphosphate to heme O; protoheme IX farnesyltransferase 299615..300463 Francisella tularensis subsp. tularensis SCHU S4 3192388 YP_169336.1 CDS lpxD2 NC_006570.2 300608 301651 R Similar to Q8A014 UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase from Bacteroides thetaiotaomicron (346 aa). FASTA: opt: 687 Z-score: 795.8 E(): 2e-36 Smith-Waterman score: 687; 33.431 identity in 341 aa overlap paralog of FTT1571; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase complement(300608..301651) Francisella tularensis subsp. tularensis SCHU S4 3190871 YP_169337.1 CDS FTT_0287c NC_006570.2 301719 302414 R Similar to Q8XIW2 Hypothetical protein CPE2001 from Clostridium perfringens (229 aa). FASTA: opt: 806 Z-score: 994.2 E(): 1.7e-47 Smith-Waterman score: 806; 51.948 identity in 231 aa overlap ORF ftt0287c; hypothetical protein complement(301719..302414) Francisella tularensis subsp. tularensis SCHU S4 3191302 YP_169338.1 CDS pdxY NC_006570.2 302414 303265 R Similar to Q8D4Q2 Pyridoxal/pyridoxine/pyridoxamine kinase from Vibrio vulnificus (290 aa). FASTA: opt: 611 Z-score: 766.0 E(): 8.9e-35 Smith-Waterman score: 611; 38.545identity in 275 aa overlap; pyridoxal kinase complement(302414..303265) Francisella tularensis subsp. tularensis SCHU S4 3191321 YP_169339.1 CDS FTT_0289c NC_006570.2 303272 303706 R ORF ftt0289c; hypothetical protein complement(303272..303706) Francisella tularensis subsp. tularensis SCHU S4 3191300 YP_169340.1 CDS moxR NC_006570.2 303852 304808 D Similar to Q87YN6 MoxR protein, , from Pseudomonas syringae (319 aa). FASTA: opt: 1309 Z-score: 1518.5 E(): 9.9e-77 Smith-Waterman score: 1309; 61.489 identity in 309 aa overlap; methanol dehydrogenase regulatory protein 303852..304808 Francisella tularensis subsp. tularensis SCHU S4 3192009 YP_169341.1 CDS FTT_0291 NC_006570.2 304821 305732 D Similar to Q87YN5 Conserved hypothetical protein from Pseudomonas syringae (314 aa). FASTA: opt: 561 Z-score: 693.3 bits: 136.4 E(): 1e-30 Smith-Waterman score: 561; 30.479 identity in 292 aa overlap ORF ftt0291; hypothetical protein 304821..305732 Francisella tularensis subsp. tularensis SCHU S4 3191299 YP_169342.1 CDS FTT_0292 NC_006570.2 305722 306198 D Similar to Q8D3W9 Conserved hypothetical protein from Vibrio vulnificus (156 aa). FASTA: opt: 228 Z-score: 276.8 E(): 1.5e-07 Smith-Waterman score: 228; 28.481 identity in 158 aa overlap ORF ftt0292; hypothetical protein 305722..306198 Francisella tularensis subsp. tularensis SCHU S4 3191297 YP_169343.1 CDS FTT_0293 NC_006570.2 306177 307196 D Similar to Q88LA6 Von Willebrand factor type A domain protein from Pseuodmonas putida (358 aa). FASTA: opt: 905 Z-score: 1015.4 E(): 1.1e-48 Smith-Waterman score: 905; 45.122 identity in 328 aa overlap ORF ftt0293; hypothetical protein 306177..307196 Francisella tularensis subsp. tularensis SCHU S4 3191295 YP_008492911.1 CDS FTT_0293a NC_006570.2 307198 308196 D hypothetical protein 307198..308196 Francisella tularensis subsp. tularensis SCHU S4 16744727 YP_169344.1 CDS FTT_0295 NC_006570.2 308992 310617 D Similar to Q8ECP0 Conserved hypothetical protein from Shewanella oneidensis (555 aa). FASTA: opt: 529 Z-score: 628.4 E(): 4.1e-27 Smith-Waterman score: 529; 24.371 identity in 517 aa overlap ORF ftt0295; hypothetical protein 308992..310617 Francisella tularensis subsp. tularensis SCHU S4 3191293 YP_169345.1 CDS pcp NC_006570.2 310618 311295 D Similar to PCP_CLOPE (Q8XKH1) Pyrrolidone-carboxylate peptidase from Clostridium perfringens (215 aa). FASTA: opt: 710 Z-score: 844.3 E(): 3.9e-39 Smith-Waterman score: 710; 52.020identity in 198 aa overlap; pyrrolidone-carboxylate peptidase 310618..311295 Francisella tularensis subsp. tularensis SCHU S4 3191452 YP_169346.1 CDS FTT_0297 NC_006570.2 311303 311785 D ORF ftt0297; cytochrome c-type biogenesis protein 311303..311785 Francisella tularensis subsp. tularensis SCHU S4 3191292 YP_169347.1 CDS holC NC_006570.2 311816 312256 D Similar to Q8XWQ9 Probable DNA polymerase III (CHI subunit) protein from Ralstonia solanacearum (142 aa). FASTA: opt: 142 Z-score: 187.9 E(): 0.013 Smith-Waterman score: 142; 30.000identity in 120 aa overlap; DNA polymerase III subunit chi 311816..312256 Francisella tularensis subsp. tularensis SCHU S4 3192526 YP_169348.1 CDS valS NC_006570.2 312388 315147 D valine--tRNA ligase; ValRS; converts valine ATP and tRNA(Val) to AMP PPi and valyl-tRNA(Val); class-I aminoacyl-tRNA synthetase type 1 subfamily; has a posttransfer editing process to hydrolyze mischarged Thr-tRNA(Val) which is done by the editing domain; valyl-tRNA synthetase 312388..315147 Francisella tularensis subsp. tularensis SCHU S4 3191933 YP_169349.1 CDS FTT_0300 NC_006570.2 315176 316030 D ORF ftt0300; hypothetical protein 315176..316030 Francisella tularensis subsp. tularensis SCHU S4 3191289 YP_169350.1 CDS FTT_0301 NC_006570.2 316103 316972 D ORF ftt0301; hypothetical protein 316103..316972 Francisella tularensis subsp. tularensis SCHU S4 3191288 YP_169351.1 CDS FTT_0302 NC_006570.2 317156 317491 D ORF ftt0302; hypothetical protein 317156..317491 Francisella tularensis subsp. tularensis SCHU S4 3191287 YP_169352.1 CDS lldD1 NC_006570.2 317488 318645 R Similar to Q9JTX1 L-lactate dehydrogenase from Neisseria meningitidis (390 aa). FASTA: opt: 1591 Z-score: 1977.5 E(): 3e-102 Smith-Waterman score: 1591; 60.789 identity in 380 aa overlap; L-lactate dehydrogenase complement(317488..318645) Francisella tularensis subsp. tularensis SCHU S4 3191464 YP_169353.1 CDS nfnB NC_006570.2 318672 319325 R catalyzes the reduction of nitroaromatic compounds such as nitrofurazone, quinones and the anti-tumor agent CB1954; NAD(P)H-dependent; oxygen insensitive; dihydropteridine reductase complement(318672..319325) Francisella tularensis subsp. tularensis SCHU S4 3191694 YP_169354.1 CDS FTT_0305 NC_006570.2 319465 319842 D Similar to Q88NZ1 MutT/nudix family protein from Pseuodomonas putida (137 aa). FASTA: opt: 224 Z-score: 293.2 E(): 1.8e-08 Smith-Waterman score: 224; 34.545 identity in 110 aa overlap ORF ftt0305; MutT/nudix family protein 319465..319842 Francisella tularensis subsp. tularensis SCHU S4 3191286 YP_169355.1 CDS fumC NC_006570.2 320015 321406 D class II family (does not require metal); tetrameric enzyme; fumarase C; reversibly converts (S)-malate to fumarate and water; functions in the TCA cycle; fumarate hydratase 320015..321406 Francisella tularensis subsp. tularensis SCHU S4 3191124 YP_169356.1 CDS gltX NC_006570.2 321536 322942 D Charges one glutamine molecule and pairs it to its corresponding RNA trinucleotide during protein translation; glutamyl-tRNA synthetase 321536..322942 Francisella tularensis subsp. tularensis SCHU S4 3191767 YP_169357.1 CDS FTT_0308 NC_006570.2 322933 323910 D ORF ftt0308; hypothetical protein 322933..323910 Francisella tularensis subsp. tularensis SCHU S4 3191285 YP_169358.1 CDS isftu2 NC_006570.2 323934 324677 R ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 transposase from Francisella tularensis (247 aa).; transposase complement(323934..324677) Francisella tularensis subsp. tularensis SCHU S4 3190862 YP_169359.1 CDS FTT_0310 NC_006570.2 324973 326427 D Similar to Q8XT33 Probable amino-acid permease transmembrane protein from Ralstonia solanacearum (543 aa). FASTA: opt: 999 Z-score: 1146.0 E(): 5.5e-5 Smith-Waterman score: 999; 34.924 identity in 461 aa overlap ORF ftt0310; amino acid permease 324973..326427 Francisella tularensis subsp. tularensis SCHU S4 3191283 YP_169360.1 CDS FTT_0311c NC_006570.2 326759 327682 R Similar to Q9KKV6 Hypothetical protein VCA0994 from Vibrio cholerae (326 aa). FASTA: opt: 558 Z-score: 686.8 E(): 2.3e-30 Smith-Waterman score: 558; 31.949 identity in 313 aa overlap ORF ftt0311c; hypothetical protein complement(326759..327682) Francisella tularensis subsp. tularensis SCHU S4 3191081 YP_169361.1 CDS folA NC_006570.2 327764 328264 R Similar to Q9K7B6 Dihydrofolate reductase from Bacillus halodurans (163 aa). FASTA: opt: 465 Z-score: 598.3 E(): 2e-25 Smith-Waterman score: 465; 41.566 identity in 166 aa overlap; dihydrofolate reductase type I complement(327764..328264) Francisella tularensis subsp. tularensis SCHU S4 3191943 YP_169362.1 CDS rpsB NC_006570.2 328496 329215 D one of the last subunits in the assembly of the 30S subunit; absence of S2 does not inhibit assembly but results in an inactive subunit; 30S ribosomal protein S2 328496..329215 Francisella tularensis subsp. tularensis SCHU S4 3191975 YP_169363.1 CDS tsf NC_006570.2 329237 330106 D EF-Ts; functions during elongation stage of protein translation; forms a dimer; associates with EF-Tu-GDP complex and promotes exchange of GDP to GTP resulting in regeneration of the active form of EF-Tu; elongation factor Ts 329237..330106 Francisella tularensis subsp. tularensis SCHU S4 3191005 YP_169364.1 CDS pyrH NC_006570.2 330110 330859 D Catalyzes the phosphorylation of UMP to UDP; uridylate kinase 330110..330859 Francisella tularensis subsp. tularensis SCHU S4 3191650 YP_169365.1 CDS frr NC_006570.2 330888 331445 D Rrf; Frr; ribosome-recycling factor; release factor 4; RF4; recycles ribosomes upon translation termination along with release factor RF-3 and elongation factor EF-G; A GTPase-dependent process results in release of 50S from 70S; inhibited by release factor RF-1; essential for viability; structurally similar to tRNAs; ribosome recycling factor 330888..331445 Francisella tularensis subsp. tularensis SCHU S4 3191428 YP_169366.1 CDS uppS NC_006570.2 331478 332251 D catalyzes the formation of undecaprenyl pyrophosphate from isopentenyl pyrophosphate; undecaprenyl pyrophosphate synthase 331478..332251 Francisella tularensis subsp. tularensis SCHU S4 3191904 YP_169367.1 CDS cdsA NC_006570.2 332265 333059 D Similar to Q88MH5 Phosphatidate cytidylyltransferase from Pseudomonas putida (271 aa). FASTA: 398 opt: 534 Z-score: 626.6 E(): 5.2e-27 Smith-Waterman score: 536; 35.926 identity in 270 aa overlap; phosphatidate cytidylyltransferase 332265..333059 Francisella tularensis subsp. tularensis SCHU S4 3190936 YP_169368.1 CDS dut NC_006570.2 333069 333515 D catalyzes the formation of dUMP from dUTP; deoxyuridine 5'-triphosphate nucleotidohydrolase 333069..333515 Francisella tularensis subsp. tularensis SCHU S4 3191551 YP_169369.1 CDS pgsA NC_006570.2 333519 334115 D Similar to Q8XFD0 Phosphotidylglycerophosphate synthetase from Salmonella typhi (182 aa). FASTA: opt: 575 Z-score: 728.4 E(): 1.1e-32 Smith-Waterman score: 618; 50.000identity in 188 aa overlap; phosphatidylglycerophosphate synthetase 333519..334115 Francisella tularensis subsp. tularensis SCHU S4 3191364 YP_169370.1 CDS rpsL NC_006570.2 334250 334624 D interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone; located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side; mutations in the S12 gene confer streptomycin resistance; 30S ribosomal protein S12 334250..334624 Francisella tularensis subsp. tularensis SCHU S4 3191538 YP_169371.1 CDS rpsG NC_006570.2 334663 335136 D binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit; 30S ribosomal protein S7 334663..335136 Francisella tularensis subsp. tularensis SCHU S4 3191970 YP_169372.1 CDS fusA NC_006570.2 335151 337265 D EF-G; promotes GTP-dependent translocation of the ribosome during translation; many organisms have multiple copies of this gene; elongation factor G 335151..337265 Francisella tularensis subsp. tularensis SCHU S4 3191127 YP_169373.1 CDS rpsJ NC_006570.2 337286 337603 D NusE; involved in assembly of the 30S subunit; in the ribosome, this protein is involved in the binding of tRNA; in Escherichia coli this protein was also found to be involved in transcription antitermination; NusB/S10 heterodimers bind boxA sequences in the leader RNA of rrn operons which is required for antitermination; binding of NusB/S10 to boxA nucleates assembly of the antitermination complex; 30S ribosomal protein S10 337286..337603 Francisella tularensis subsp. tularensis SCHU S4 3191189 YP_169374.1 CDS rplC NC_006570.2 337707 338342 D binds directly near the 3' end of the 23S rRNA, where it nucleates assembly of the 50S subunit; essential for peptidyltransferase activity; mutations in this gene confer resistance to tiamulin; 50S ribosomal protein L3 337707..338342 Francisella tularensis subsp. tularensis SCHU S4 3192021 YP_169375.1 CDS rplD NC_006570.2 338372 338995 D L4 is important during the early stages of 50S assembly; it initially binds near the 5' end of the 23S rRNA; 50S ribosomal protein L4 338372..338995 Francisella tularensis subsp. tularensis SCHU S4 3192020 YP_169376.1 CDS rplW NC_006570.2 338992 339291 D binds third domain of 23S rRNA and protein L29; part of exit tunnel; 50S ribosomal protein L23 338992..339291 Francisella tularensis subsp. tularensis SCHU S4 3191999 YP_169377.1 CDS rplB NC_006570.2 339313 340137 D one of the primary rRNA-binding proteins; required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation; 50S ribosomal protein L2 339313..340137 Francisella tularensis subsp. tularensis SCHU S4 3192022 YP_169378.1 CDS rpsS NC_006570.2 340152 340430 D protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA; 30S ribosomal protein S19 340152..340430 Francisella tularensis subsp. tularensis SCHU S4 3192508 YP_169379.1 CDS rplV NC_006570.2 340446 340781 D binds specifically to 23S rRNA during the early stages of 50S assembly; makes contact with all 6 domains of the 23S rRNA in the assembled 50S subunit and ribosome; mutations in this gene result in erythromycin resistance; located near peptidyl-transferase center; 50S ribosomal protein L22 340446..340781 Francisella tularensis subsp. tularensis SCHU S4 3192000 YP_169380.3 CDS rpsC NC_006570.2 340796 341464 D forms a complex with S10 and S14; binds the lower part of the 30S subunit head and the mRNA in the complete ribosome to position it for translation; 30S ribosomal protein S3 340796..341464 Francisella tularensis subsp. tularensis SCHU S4 3191974 YP_169381.1 CDS rplP NC_006570.2 341468 341881 D located in the peptidyl transferase center and may be involved in peptidyl transferase activity; similar to archaeal L10e; 50S ribosomal protein L16 341468..341881 Francisella tularensis subsp. tularensis SCHU S4 3192008 YP_169382.1 CDS rpmC NC_006570.2 341881 342081 D one of the stabilizing components for the large ribosomal subunit; 50S ribosomal protein L29 341881..342081 Francisella tularensis subsp. tularensis SCHU S4 3191993 YP_169383.1 CDS rpsQ NC_006570.2 342094 342345 D primary binding protein; helps mediate assembly; involved in translation fidelity; 30S ribosomal protein S17 342094..342345 Francisella tularensis subsp. tularensis SCHU S4 3191023 YP_169384.1 CDS rplN NC_006570.2 342437 342805 D binds to the 23S rRNA between the centers for peptidyl transferase and GTPase; 50S ribosomal protein L14 342437..342805 Francisella tularensis subsp. tularensis SCHU S4 3192011 YP_169385.1 CDS rplX NC_006570.2 342827 343144 D assembly initiator protein; binds to 5' end of 23S rRNA and nucleates assembly of the 50S; surrounds polypeptide exit tunnel; 50S ribosomal protein L24 342827..343144 Francisella tularensis subsp. tularensis SCHU S4 3191998 YP_169386.1 CDS rplE NC_006570.2 343156 343695 D part of 50S and 5S/L5/L18/L25 subcomplex; contacts 5S rRNA and P site tRNA; forms a bridge to the 30S subunit in the ribosome by binding to S13; 50S ribosomal protein L5 343156..343695 Francisella tularensis subsp. tularensis SCHU S4 3192018 YP_169387.1 CDS rpsN NC_006570.2 343714 344019 D located in the peptidyl transferase center and involved in assembly of 30S ribosome subunit; similar to what is observed with proteins L31 and L33, some proteins in this family contain CXXC motifs that are involved in zinc binding; if two copies are present in a genome, then the duplicated copy appears to have lost the zinc-binding motif and is instead regulated by zinc; the proteins in this group do not appear to have the zinc-binding motif; 30S ribosomal protein S14 343714..344019 Francisella tularensis subsp. tularensis SCHU S4 3192379 YP_169388.1 CDS rpsH NC_006570.2 344036 344434 D binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit; 30S ribosomal protein S8 344036..344434 Francisella tularensis subsp. tularensis SCHU S4 3190958 YP_169389.1 CDS rplF NC_006570.2 344453 344989 D ribosomal protein L6 appears to have arisen as a result of an ancient gene duplication as based on structural comparison of the Bacillus stearothermophilus protein; RNA-binding appears to be in the C-terminal domain; mutations in the L6 gene confer resistance to aminoglycoside antibiotics such as gentamicin and these occur in truncations of the C-terminal domain; it has been localized to a region between the base of the L7/L12 stalk and the central protuberance; 50S ribosomal protein L6 344453..344989 Francisella tularensis subsp. tularensis SCHU S4 3192017 YP_169390.1 CDS rplR NC_006570.2 345012 345365 D binds 5S rRNA along with protein L5 and L25; 50S ribosomal protein L18 345012..345365 Francisella tularensis subsp. tularensis SCHU S4 3192006 YP_169391.1 CDS rpsE NC_006570.2 345393 345893 D located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body; contacts S4 and S8; with S4 and S12 plays a role in translational accuracy; mutations in this gene result in spectinomycin resistance; 30S ribosomal protein S5 345393..345893 Francisella tularensis subsp. tularensis SCHU S4 3191972 YP_169392.1 CDS rpmD NC_006570.2 345900 346085 D L30 binds domain II of the 23S rRNA and the 5S rRNA; similar to eukaryotic protein L7; 50S ribosomal protein L30 345900..346085 Francisella tularensis subsp. tularensis SCHU S4 3191991 YP_169393.1 CDS rplO NC_006570.2 346092 346523 D late assembly protein; 50S ribosomal protein L15 346092..346523 Francisella tularensis subsp. tularensis SCHU S4 3192010 YP_169394.1 CDS secY NC_006570.2 346534 347859 D forms heterotrimeric complex in the membrane; in bacteria the complex consists of SecY which forms the channel pore and SecE and SecG; the SecG subunit is not essential; in bacteria translocation is driven via the SecA ATPase; preprotein translocase subunit SecY 346534..347859 Francisella tularensis subsp. tularensis SCHU S4 3190709 YP_169395.1 CDS rpmJ NC_006570.2 347883 347996 D smallest protein in the large subunit; similar to what is found with protein L31 and L33 several bacterial genomes contain paralogs which may be regulated by zinc; the protein from Thermus thermophilus has a zinc-binding motif and contains a bound zinc ion; the proteins in this group have the motif; 50S ribosomal protein L36 347883..347996 Francisella tularensis subsp. tularensis SCHU S4 3191985 YP_169396.1 CDS rpsM NC_006570.2 348113 348469 D located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA; makes contact with the large subunit via RNA-protein interactions and via protein-protein interactions with L5; contacts P-site tRNA; 30S ribosomal protein S13 348113..348469 Francisella tularensis subsp. tularensis SCHU S4 3191860 YP_169397.1 CDS rpsK NC_006570.2 348509 348898 D located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA; forms part of the Shine-Dalgarno cleft in the 70S ribosome; interacts with S7 and S18 and IF-3; 30S ribosomal protein S11 348509..348898 Francisella tularensis subsp. tularensis SCHU S4 3190768 YP_169398.1 CDS rpsD NC_006570.2 348920 349540 D primary rRNA binding protein; nucleates 30S assembly; involved in translational accuracy with proteins S5 and S12; interacts with protein S5; involved in autogeneously regulating ribosomal proteins by binding to pseudoknot structures in the polycistronic mRNA; interacts with transcription complex and functions similar to protein NusA in antitermination; 30S ribosomal protein S4 348920..349540 Francisella tularensis subsp. tularensis SCHU S4 3191973 YP_169399.1 CDS rpoA1 NC_006570.2 349598 350569 D catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Dimerization of the alpha subunit is the first step in the sequential assembly of subunits to form the holoenzyme; DNA-directed RNA polymerase subunit alpha 349598..350569 Francisella tularensis subsp. tularensis SCHU S4 3191984 YP_169400.1 CDS rplQ NC_006570.2 350614 351051 D is a component of the macrolide binding site in the peptidyl transferase center; 50S ribosomal protein L17 350614..351051 Francisella tularensis subsp. tularensis SCHU S4 3192007 YP_169401.1 CDS isftu1 NC_006570.2 351205 351989 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(351205..351531,351531..351989) Francisella tularensis subsp. tularensis SCHU S4 3192216 YP_169402.1 CDS FTT_0354 NC_006570.2 352918 353913 D Duplicate of FTT0378 and FTT1263 ORF ftt0354; hypothetical protein 352918..353913 Francisella tularensis subsp. tularensis SCHU S4 3191453 YP_169403.1 CDS isftu1 NC_006570.2 354205 354989 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(354205..354663,354663..354989)) Francisella tularensis subsp. tularensis SCHU S4 3191882 YP_169404.1 CDS htpG NC_006570.2 355151 357037 D molecular chaperone; heat shock protein 90 355151..357037 Francisella tularensis subsp. tularensis SCHU S4 3191490 YP_169405.1 CDS isftu1 NC_006570.2 357361 358145 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(357361..357819,357819..358145)) Francisella tularensis subsp. tularensis SCHU S4 3192206 YP_169406.1 CDS FTT_0359 NC_006570.2 359413 359883 D ORF ftt0359; hypothetical protein 359413..359883 Francisella tularensis subsp. tularensis SCHU S4 3191450 YP_169407.1 CDS FTT_0360 NC_006570.2 360020 360844 D Similar to Q82V84 Short-chain dehydrogenase/reductase (SDR) superfamily from Nitrosomonas europaea (279 aa). FASTA: opt: 829 Z-score: 982.8 E(): 7.5e-47 Smith-Waterman score: 829; 49.632 identity in 272 aa overlap ORF ftt0360; short-chain dehydrogenase 360020..360844 Francisella tularensis subsp. tularensis SCHU S4 3191448 YP_169408.1 CDS FTT_0361c NC_006570.2 360866 362215 R Similar to Q8Z506 amino acid transporter from Salmonella typhi (473 aa). FASTA: opt: 717 Z-score: 754.8 E(): 3.8e-34 Smith-Waterman score: 755; 29.783 identity in 460 aa overlap ORF ftt0361c; amino acid transporter complement(360866..362215) Francisella tularensis subsp. tularensis SCHU S4 3191447 YP_169409.1 CDS FTT_0362c NC_006570.2 362474 363346 R ORF ftt0362c; hypothetical protein complement(362474..363346) Francisella tularensis subsp. tularensis SCHU S4 3191445 YP_169410.1 CDS isftu1 NC_006570.2 363514 364298 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(363514..363840,363840..364298) Francisella tularensis subsp. tularensis SCHU S4 3192291 YP_169411.1 CDS FTT_0364c NC_006570.2 364422 364871 R ORF ftt0364c; hypothetical protein complement(364422..364871) Francisella tularensis subsp. tularensis SCHU S4 3191446 YP_169412.1 CDS poxF NC_006570.2 365352 366083 D Similar to Q8PFB8 Phenol hydroxylase from Xanthomonas axonopodis (365 aa). FASTA: opt: 567 Z-score: 717.5 E(): 4.5e-32 Smith-Waterman score: 567; 38.211 identity in 246 aa overlap The protein seems to be shorter (<115 aa) than the matching proteins.; phenol hydroxylase 365352..366083 Francisella tularensis subsp. tularensis SCHU S4 3191544 YP_169413.1 CDS rpmE NC_006570.2 366205 366420 D RpmE; there appears to be two types of ribosomal proteins L31 in bacterial genomes; some contain a CxxC motif while others do not; Bacillus subtilis has both types; the proteins in this cluster have the CXXC motif; RpmE is found in exponentially growing Bacilli while YtiA was found after exponential growth; expression of ytiA is controlled by a zinc-specific transcriptional repressor; RpmE contains one zinc ion and a CxxC motif is responsible for this binding; forms an RNP particle along with proteins L5, L18, and L25 and 5S rRNA; found crosslinked to L2 and L25 and EF-G; may be near the peptidyltransferase site of the 50S ribosome; 50S ribosomal protein L31 366205..366420 Francisella tularensis subsp. tularensis SCHU S4 3191990 YP_169414.1 CDS gshA NC_006570.2 366631 368136 R Similar to Q88AR1 Glutamate--cysteine ligase from Pseudomonas syringae (529 aa). FASTA: opt: 1113 Z-score: 1281.1 E(): 1.8e-63 Smith-Waterman score: 1113; 39.250 identity in 507 aa overlap; glutamate--cysteine ligase complement(366631..368136) Francisella tularensis subsp. tularensis SCHU S4 3192547 YP_169415.1 CDS mviN NC_006570.2 368189 369733 R Similar to AAO89943 (Q83ED5) Integral membrane protein MviN from Coxiella burnetii (515 aa). FASTA: opt: 836 Z-score: 887.1 E(): 1.6e-41 Smith-Waterman score: 836; 30.452 identity in 509 aa overlap; virulence factor MviN complement(368189..369733) Francisella tularensis subsp. tularensis SCHU S4 3192066 YP_169416.1 CDS FTT_0369c NC_006570.2 369756 370817 R ORF ftt0369c; hypothetical protein complement(369756..370817) Francisella tularensis subsp. tularensis SCHU S4 3191442 YP_169417.1 CDS yjeE NC_006570.2 370900 371310 R Similar to Q8Z189 Hypothetical protein yjeE from Salmonella typhi (153 aa). FASTA: opt: 517 Z-score: 623.3 E(): 7.9e-27 Smith-Waterman score: 517; 56.489 identity in 131 aa overlap; nucleotide-binding protein complement(370900..371310) Francisella tularensis subsp. tularensis SCHU S4 3192380 YP_169418.1 CDS folC NC_006570.2 371288 372469 R Similar to Q8ECR5 FolC bifunctional protein from Shewanella oneidensis (431 aa). FASTA: opt: 507 Z-score: 630.2 E(): 3.3e-27 Smith-Waterman score: 627; 32.274 identity in 409 aa overlap; bifunctional folylpolyglutamate synthase/ dihydrofolate synthase complement(371288..372469) Francisella tularensis subsp. tularensis SCHU S4 3191937 YP_169419.1 CDS accD NC_006570.2 372484 373392 R Similar to Q8XFJ5 Acetyl-CoA carboxylase beta subunit from Salmonella typhi (304 aa). FASTA: opt: 1197 Z-score: 1430.2 E(): 9.1e-72 Smith-Waterman score: 1197; 62.116 identity in 293 aa overlap; acetyl-Coa carboxylase beta subunit complement(372484..373392) Francisella tularensis subsp. tularensis SCHU S4 3191466 YP_169420.1 CDS ndk NC_006570.2 373428 373850 R catalyzes the formation of nucleoside triphosphate from ATP and nucleoside diphosphate; nucleoside diphosphate kinase complement(373428..373850) Francisella tularensis subsp. tularensis SCHU S4 3191686 YP_169421.1 CDS pyrG NC_006570.2 373923 375563 R CTP synthase; cytidine triphosphate synthetase; catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; in Escherichia coli this enzyme forms a homotetramer; CTP synthetase complement(373923..375563) Francisella tularensis subsp. tularensis SCHU S4 3191651 YP_169422.1 CDS FTT_0376c NC_006570.2 377481 378539 R Weak hits at position aa 250-350 to many cytochrome oxidase subunit I eg:(Q85L50). ORF ftt0376c; hypothetical protein complement(377481..378539) Francisella tularensis subsp. tularensis SCHU S4 3191440 YP_169423.1 CDS isftu1 NC_006570.2 378906 379690 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(378906..379235,379235..379690) Francisella tularensis subsp. tularensis SCHU S4 3192115 YP_169424.1 CDS FTT_0378c NC_006570.2 379982 380977 R Duplicate of FTT0354 and FTT1263 ORF ftt0378c; hypothetical protein complement(379982..380977) Francisella tularensis subsp. tularensis SCHU S4 3191439 YP_169425.1 CDS gdh NC_006570.2 382014 383363 R converts 2-oxoglutarate to glutamate; in Escherichia coli this enzyme plays a role in glutamate synthesis when the cell is under energy restriction; uses NADPH; forms a homohexamer; glutamate dehydrogenase complement(382014..383363) Francisella tularensis subsp. tularensis SCHU S4 3191583 YP_169426.1 CDS isftu2 NC_006570.2 383702 384445 D ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 transposase from Francisella tularensis (247 aa).; transposase 383702..384445 Francisella tularensis subsp. tularensis SCHU S4 3192483 YP_169427.1 CDS FTT_0382 NC_006570.2 384570 384674 D ORF ftt0382; hypothetical protein 384570..384674 Francisella tularensis subsp. tularensis SCHU S4 3191437 YP_169428.1 CDS FTT_0383 NC_006570.2 385685 386020 D ORF ftt0383; hypothetical protein 385685..386020 Francisella tularensis subsp. tularensis SCHU S4 3191438 YP_169429.2 CDS psd NC_006570.2 385993 386844 R catalyzes the decarboxylation of phosphatidyl-L-serine to phosphatidylethanoleamine; phosphatidylserine decarboxylase complement(385993..386844) Francisella tularensis subsp. tularensis SCHU S4 3191677 YP_169430.1 CDS FTT_0385 NC_006570.2 386944 387924 D ORF ftt0385; hypothetical protein 386944..387924 Francisella tularensis subsp. tularensis SCHU S4 3191436 YP_169431.1 CDS nadM NC_006570.2 387933 388976 D NadM-Nudix subfamily; involved in creation of nicotanimide adenine dinucleotide NAD from either biosynthetic or salvage pathways; bifunctional nicotinamide mononucleotide adenylyltransferase/ADP-ribose pyrophosphatase 387933..388976 Francisella tularensis subsp. tularensis SCHU S4 3192435 YP_169432.1 CDS glmU NC_006570.2 388990 390357 D Similar to Q9KNH7 UDP-N-acetylglucosamine pyrophosphorylase from Vibrio cholerae (453 aa). FASTA: opt: 1597 Z-score: 1863.6 E(): 6.5e-96 Smith-Waterman score: 1597; 53.201 identity in 453 aa overlap; UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase 388990..390357 Francisella tularensis subsp. tularensis SCHU S4 3191075 YP_169433.1 CDS glmS NC_006570.2 390378 392216 D Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source; glucosamine--fructose-6-phosphate aminotransferase 390378..392216 Francisella tularensis subsp. tularensis SCHU S4 3192352 YP_169434.1 CDS FTT_0389 NC_006570.2 392300 392824 D Similar to Q9RWR2 Acetyltransferase, , from Deinococcus radiodurans (188 aa). FASTA: opt: 188 Z-score: 245.7 E(): 8.5e-06 Smith-Waterman score: 188; 28.105 identity in 153 aa overlap ORF ftt0389; acetyltransferase 392300..392824 Francisella tularensis subsp. tularensis SCHU S4 3191435 YP_169435.1 CDS rpsU NC_006570.2 392817 393014 R a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA; 30S ribosomal protein S21 complement(392817..393014) Francisella tularensis subsp. tularensis SCHU S4 3191264 YP_169436.1 CDS cspC NC_006570.2 393014 393217 R Similar to Q8D6M5 Cold shock protein from Vibrio vulnificus (70 aa). FASTA: opt: 272 Z-score: 403.6 E(): 1.4e-14 Smith-Waterman score: 272; 58.571 identity in 70 aa overlap; cold shock protein complement(393014..393217) Francisella tularensis subsp. tularensis SCHU S4 3191899 YP_169437.1 CDS FTT_0392c NC_006570.2 393465 393794 R Similar to Q8TT97 Hypothetical protein MA0540 from Methanosarcina mazei (95 aa). FASTA: opt: 151 Z-score: 197.5 E(): 0.0038 Smith-Waterman score: 151; 35.789 identity in 95 aa overlap ORF ftt0392c; hypothetical protein complement(393465..393794) Francisella tularensis subsp. tularensis SCHU S4 3191434 YP_169438.1 CDS map NC_006570.2 393921 394691 D Similar to Q886P4 Methionine aminopeptidase,type I from Pseudomonas syringae (260 aa). FASTA: opt: 1243 Z-score: 1568.2 E(): 1.9e-79 Smith-Waterman score: 1243; 70.120 identity in 251 aa overlap; methionine aminopeptidase 393921..394691 Francisella tularensis subsp. tularensis SCHU S4 3191507 YP_169439.1 CDS FTT_0394 NC_006570.2 394693 395541 D ORF ftt0394; hypothetical protein 394693..395541 Francisella tularensis subsp. tularensis SCHU S4 3191432 YP_169440.1 CDS FTT_0395 NC_006570.2 395587 396300 D Similar to Q9L5J1 hypothetical protein from Salmonella typhi (259 aa). FASTA: opt: 585 Z-score: 707.5 E(): 1.6e-31 Smith-Waterman score: 680; 46.743 identity in 261 aa overlap ORF ftt0395; hypothetical protein 395587..396300 Francisella tularensis subsp. tularensis SCHU S4 3191429 YP_169441.1 CDS parC NC_006570.2 396433 398658 D decatenates newly replicated chromosomal DNA and relaxes positive and negative DNA supercoiling; DNA topoisomerase IV subunit A 396433..398658 Francisella tularensis subsp. tularensis SCHU S4 3191415 YP_169442.1 CDS mtn NC_006570.2 398658 399344 D enables the cleavage of the glycosidic bond in both 5'-methylthioadenosine and S-adenosylhomocysteine; 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 398658..399344 Francisella tularensis subsp. tularensis SCHU S4 3191248 YP_169443.1 CDS FTT_0398c NC_006570.2 399331 399930 R ORF ftt0398c; hypothetical protein complement(399331..399930) Francisella tularensis subsp. tularensis SCHU S4 3191430 YP_169444.1 CDS FTT_0399c NC_006570.2 399923 401041 R Similar to Q885D8 BNR/Asp-box repeat protein from Pseudomonas syringae (432 aa). FASTA: opt: 536 Z-score: 654.4 E(): 1.5e-28 Smith-Waterman score: 536; 31.922 identity in 307 aa overlap ORF ftt0399c; BNR/Asp-box repeat-containing protein complement(399923..401041) Francisella tularensis subsp. tularensis SCHU S4 3191433 YP_169445.1 CDS slt NC_006570.2 401240 403216 D Similar to Q88L07 Soluble lytic transglycosylase, from Pseudomonas putida (657 aa). FASTA: opt: 596 Z-score: 646.0 E(): 3.9e-28 Smith-Waterman score: 819; 27.822 identity in 629 aa overlap.; soluble lytic murein transglycosylase 401240..403216 Francisella tularensis subsp. tularensis SCHU S4 3190706 YP_169446.1 CDS FTT_0401 NC_006570.2 403506 404228 D Partial homology to carboxy terminus of Q9CLV4a Hypothetical protein PM1097 from Pasteurella multocida (417 aa). FASTA: opt: 269 Z-score: 330.3 E(): 1.7e-10 Smith-Waterman score: 269; 26.050identity in 238 aa overlap ORF ftt0401; hypothetical protein 403506..404228 Francisella tularensis subsp. tularensis SCHU S4 3191427 YP_169447.1 CDS dnaE NC_006570.2 404585 408064 D Similar to DP3A_SALTY (P14567) DNA polymerase III alpha subunit from Salmonella typhimurium (1160 aa). FASTA: opt: 3099 Z-score: 3288.3 E(): 2.6e-175 Smith-Waterman score: 3099; 44.781identity in 1121 aa overlap.; DNA polymerase III subunit alpha 404585..408064 Francisella tularensis subsp. tularensis SCHU S4 3191527 YP_169448.1 CDS def1 NC_006570.2 408140 408775 D Similar to DEF_LEPIN (Q93LE9) Peptide deformylase from Leptospira interrogans (178 aa). FASTA: opt: 312 Z-score: 399.2 E(): 2.4e-14 Smith-Waterman score: 312; 35.758 identity in 165 aa overlap; peptide deformylase 408140..408775 Francisella tularensis subsp. tularensis SCHU S4 3191512 YP_169449.1 CDS lolC NC_006570.2 409479 410741 D Similar to Q83CV1 lipoprotein ABC transporter,permease protein from Coxiella burnetii (414 aa). FASTA: opt: 1273 Z-score: 1436.4 E(): 4.1e-72 Smith-Waterman score: 1273; 48.104 identity in 422 aa overlap; lipoprotein releasing system membrane protein 409479..410741 Francisella tularensis subsp. tularensis SCHU S4 3192203 YP_169450.1 CDS lolD NC_006570.2 410734 411429 D Similar to LOLD_NEIMA (P57030) Lipoprotein releasing system ATP-binding transport protein from Neisseria meningitidis (231 aa). FASTA: opt: 791 Z-score: 892.8 E(): 7e-42 Smith-Waterman score: 791; 51.770 identity in 226 aa overlap; lipoprotein releasing system ABC transporter ATP-binding protein 410734..411429 Francisella tularensis subsp. tularensis SCHU S4 3192199 YP_169451.1 CDS cadA NC_006570.2 411546 413687 D Similar to DCLY_ECOLI (P23892) Lysine decarboxylase, inducible from E. coli (715 aa) FASTA: opt: 2608 Z-score: 3095.0 E(): 1.7e-164 Smith-Waterman score: 2608; 53.912 identity in 703 aa overlap; lysine decarboxylase, inducable 411546..413687 Francisella tularensis subsp. tularensis SCHU S4 3191271 YP_169452.1 CDS gcvT NC_006570.2 413779 414855 D catalyzes the transfer of a methylene carbon from the methylamine-loaded GcvH protein to tetrahydrofolate, causing the release of ammonia and the generation of reduced GcvH protein; glycine cleavage system aminomethyltransferase T 413779..414855 Francisella tularensis subsp. tularensis SCHU S4 3192518 YP_169453.1 CDS gcvH1 NC_006570.2 414911 415294 D Similar to Q8FE66 Glycine cleavage system H protein from E coli (130 aa). FASTA: opt: 526 Z-score: 699.9 E(): 4.3e-31 Smith-Waterman score: 526; 60.938 identity in 128 aa overlap; glycine cleavage system H protein 414911..415294 Francisella tularensis subsp. tularensis SCHU S4 3191553 YP_169454.1 CDS gcvP1 NC_006570.2 415358 416725 D acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine; forms a heterodimer with subunit 2 to form the P protein; glycine dehydrogenase subunit 1 415358..416725 Francisella tularensis subsp. tularensis SCHU S4 3192502 YP_169455.1 CDS gcvP2 NC_006570.2 416731 418176 D acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine; forms a heterodimer with subunit 1 to form the P protein; glycine dehydrogenase subunit 2 416731..418176 Francisella tularensis subsp. tularensis SCHU S4 3192315 YP_169456.1 CDS aroE2 NC_006570.2 418296 419141 R Similar to Q8FQC7 shikimate 5-dehydrogenase from Corynebacterium efficiens(269 aa). FASTA: opt: 283 Z-score: 321.7 E(): 4.6e-10 Smith-Waterman score: 287; 28.077 identity in 260 aa overlap.; shikimate 5-dehydrogenase complement(418296..419141) Francisella tularensis subsp. tularensis SCHU S4 3190949 YP_169457.1 CDS pulB NC_006570.2 419167 422379 R Similar to Q8XK16 Pullulanase from Clostridium perfringens (1064 aa). FASTA: opt: 3419 Z-score: 3958.4 E(): 1.4e-212 Smith-Waterman score: 3419; 48.369identity in 1042 aa overlap Other FASTA hits have lower scores due to generally being smaller in size. Q8XK16 is an electronic annotation; pullulonase complement(419167..422379) Francisella tularensis subsp. tularensis SCHU S4 3191967 YP_169458.1 CDS glgB NC_006570.2 422413 424335 R catalyzes the transfer of a segment of a 1,4-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain; glycogen branching protein complement(422413..424335) Francisella tularensis subsp. tularensis SCHU S4 3190770 YP_169459.1 CDS pgm NC_006570.2 424637 426271 D catalyzes the interconversion of alpha-D-glucose 1-phosphate to alpha-D-glucose 6-phosphate; phosphoglucomutase 424637..426271 Francisella tularensis subsp. tularensis SCHU S4 3191363 YP_169460.1 CDS glgA NC_006570.2 427587 429056 D catalyzes the formation of alpha-1,4-glucan chains from ADP-glucose; glycogen synthase 427587..429056 Francisella tularensis subsp. tularensis SCHU S4 3192504 YP_169461.1 CDS malP NC_006570.2 429161 431434 D Similar to Q8DN49 Maltodextrin phosphorylase from Streptococcus pneumoniae (752 aa). FASTA: opt: 2938 Z-score: 3443.0 E(): 6.9e-184 Smith-Waterman score: 2938; 59.569 identity in 742 aa overlap; maltodextrin phosphorylase 429161..431434 Francisella tularensis subsp. tularensis SCHU S4 3191867 YP_169462.1 CDS malA NC_006570.2 431478 432941 D amylomaltase; acts to release glucose from maltodextrins; 4-alpha-glucanotransferase 431478..432941 Francisella tularensis subsp. tularensis SCHU S4 3192205 YP_169463.1 CDS glyQ NC_006570.2 433015 433923 D glycine--tRNA ligase alpha chain; GlyRS; class II aminoacyl tRNA synthetase; tetramer of alpha(2)beta(2); catalyzes a two-step reaction; first charging a glycine molecule by linking its carboxyl group to the alpha-phosphate of ATP; second by transfer of the aminoacyl-adenylate to its tRNA; glycyl-tRNA synthetase subunit alpha 433015..433923 Francisella tularensis subsp. tularensis SCHU S4 3191773 YP_169464.1 CDS murE NC_006570.2 434134 435573 D Similar to MURE_NEIMB (Q9K0Y9) UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase from Neisseria meningitidis (492 aa). FASTA: opt: 1037 Z-score: 1223.7, E(): 2.9e-60 Smith-Waterman score: 1037; 40.795 identity in 478 aa overlap; UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase 434134..435573 Francisella tularensis subsp. tularensis SCHU S4 3191957 YP_169465.1 CDS murF NC_006570.2 436365 437723 D Similar to Q87SG8 UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate -D-alanyl-D-alanyl ligase from Vibrio parahaemolyticus (454 aa). FASTA: opt: 870 Z-score: 1005.8 E(): 3.9e-48 Smith-Waterman score: 870; 35.011 identity in 457 aa overlap; UDP-N--acetylmuramoylalanyl-D-glutamyl-2,6- diaminopimelate-D-alanyl-D-alanyl ligase 436365..437723 Francisella tularensis subsp. tularensis SCHU S4 3191072 YP_169466.1 CDS FTT_0423 NC_006570.2 437732 437911 D ORF ftt0423; hypothetical protein 437732..437911 Francisella tularensis subsp. tularensis SCHU S4 3191424 YP_169468.1 CDS asd NC_006570.2 438607 439701 R catalyzes the formation of 4-aspartyl phosphate from aspartate 4-semialdehyde; aspartate-semialdehyde dehydrogenase complement(438607..439701) Francisella tularensis subsp. tularensis SCHU S4 3190952 YP_169469.1 CDS thrC1 NC_006570.2 443011 444297 D catalyzes the formation of L-threonine from O-phospho-L-homoserine; threonine synthase 443011..444297 Francisella tularensis subsp. tularensis SCHU S4 3190776 YP_169470.1 CDS speH NC_006570.2 444938 445372 D Similar to SPEH_METJA (Q57763) S-adenosylmethionine decarboxylase proenzyme from Methanococcus jannaschii (124 aa). FASTA: opt: 355 Z-score: 487.2, E(): 3e-19 Smith-Waterman score: 355; 44.737 identity in 114 aa overlap; S-adenosylmethionine decarboxylase 444938..445372 Francisella tularensis subsp. tularensis SCHU S4 3190726 YP_169471.1 CDS speE NC_006570.2 445372 446241 D catalyzes the formation of spermidine from putrescine and S-adenosylmethioninamine; spermidine synthase 445372..446241 Francisella tularensis subsp. tularensis SCHU S4 3190722 YP_169472.1 CDS speA NC_006570.2 446267 447676 D Similar to Q8A2B1 arginine decarboxylase from Bacteroides thetaiotamicron (630 aa). FASTA: opt: 571 Z-score: 678.2 E(): 6.9e-30 Smith-Waterman score: 689; 28.755 identity in 546 aa overlap; arginine decarboxylase 446267..447676 Francisella tularensis subsp. tularensis SCHU S4 3190717 YP_169473.1 CDS FTT_0433 NC_006570.2 447726 447914 D ORF ftt0433; hypothetical protein 447726..447914 Francisella tularensis subsp. tularensis SCHU S4 3191423 YP_169474.1 CDS FTT_0434 NC_006570.2 447895 448881 D Similar to Q8KCB6 Hypothetical protein CT1508 from Chlorobium tepidum (347 aa). FASTA: opt: 742 Z-score: 939.4 E(): 2e-44 Smith-Waterman score: 837; 38.484 identity in 343 aa overlap PAD_porph is a possible virulence factor for P. gingivalis: PMID: 10377098 ORF ftt0434; hypothetical protein 447895..448881 Francisella tularensis subsp. tularensis SCHU S4 3191422 YP_169475.1 CDS FTT_0435 NC_006570.2 448883 449743 D Similar to Q87NU4 carbon-nitrogen hydrolase from Vibrio parahaemolyticus (288 aa). FASTA: opt: 1139 Z-score: 1419.0 E(): 3.8e-71 Smith-Waterman score: 1139; 57.971 identity in 276 aa overlap. Possible candidate for citrulline ureidase ORF ftt0435; carbon-nitrogen hydrolase family protein 448883..449743 Francisella tularensis subsp. tularensis SCHU S4 3191421 YP_169476.1 CDS lpxH NC_006570.2 449760 450476 R catalyzes the formation of 2,3=diacylglucosamine 1-phosphate from UDP-2,3=diacylglucosamine; UDP-2,3-diacylglucosamine hydrolase complement(449760..450476) Francisella tularensis subsp. tularensis SCHU S4 3191794 YP_169477.1 CDS pyrE NC_006570.2 450466 451092 R involved in fifth step of pyrimidine biosynthesis; converts orotidine 5'-phosphate and diphosphate to orotate and 5-phospho-alpha-D-ribose 1-diphosphate; orotate phosphoribosyltransferase complement(450466..451092) Francisella tularensis subsp. tularensis SCHU S4 3191653 YP_169478.1 CDS mpl NC_006570.2 451252 452622 D Similar to MPL_ECOLI (P37773) UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso- diamino pimelate ligase (murein peptide ligase) from E coli (457 aa). FASTA: opt: 1392, Z-score: 1656.5, E(): 2.3e-84,Smith-Waterman score: 1392; 47.912 identity in 455 aa overlap; UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase 451252..452622 Francisella tularensis subsp. tularensis SCHU S4 3191609 YP_169479.1 CDS yjfH NC_006570.2 452619 453275 D Similar to YJFH_ECOLI (P39290) hypothetical tRNA/rRNA methyltransferase yjfH from E. coli (243 aa). FASTA: opt: 556, Z-score: 695.5, E(): 7.5e-31 Smith-Waterman score: 556; 43.128 identity in 211 aa overlap. This CDS is disrupted by the insertion of an ISFtu1 element close to the C-terminal. It is not clear whether this insertion affects the function of the protein; tRNA/rRNA methyltransferase 452619..453275 Francisella tularensis subsp. tularensis SCHU S4 3192391 YP_169480.1 CDS isftu1 NC_006570.2 453349 454133 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(453349..453807,453807..454133)) Francisella tularensis subsp. tularensis SCHU S4 3191789 YP_169481.1 CDS FTT_0442c NC_006570.2 455004 456197 R Similar to Q8EU72 Bicyclomycin resistance protein from Oceanobacillus iheyensis (412 aa). FASTA: opt: 614 Z-score: 668.2 E(): 2.5e-29 Smith-Waterman score: 614; 30.179identity in 391 aa overlap ORF ftt0442c; major facilitator transporter complement(455004..456197) Francisella tularensis subsp. tularensis SCHU S4 3191419 YP_169482.1 CDS FTT_0443 NC_006570.2 456469 457512 D Similar to Q83C03 Conserved domain protein from Coxiella burnetii (377 aa). FASTA: opt: 359 Z-score: 409.9 E(): 6.1e-15 Smith-Waterman score: 380; 27.350 identity in 351 aa overlap ORF ftt0443; hypothetical protein 456469..457512 Francisella tularensis subsp. tularensis SCHU S4 3191417 YP_169483.1 CDS tet NC_006570.2 457583 458821 D Similar to Q83CB9 Multidrug transporter, from Coxiella burnetii (412 aa). FASTA: opt: 558 Z-score: 638.9 E(): 1.1e-27 Smith-Waterman score: 558; 25.854identity in 410 aa overlap. Similar to tetA and tetC which are situated on a plasmid in E. coli; multidrug transporter 457583..458821 Francisella tularensis subsp. tularensis SCHU S4 3190751 YP_169484.1 CDS FTT_0446 NC_006570.2 460752 462194 D Similar to Q83RB7 transport protein from Shigella flexneri (500 aa). FASTA: opt: 567 Z-score: 573.2 E(): 4.9e-24 Smith-Waterman score: 578; 26.122 identity in 490 aa overlap ORF ftt0446; proton-dependent oligopeptide transport (POT) family protein 460752..462194 Francisella tularensis subsp. tularensis SCHU S4 3191413 YP_169485.1 CDS FTT_0447c NC_006570.2 462338 463678 R Similar to Q83DW3 Hypothetical protein from Coxiella burnetii (497 aa). FASTA: opt: 334 Z-score: 356.6 E(): 5.7e-12 Smith-Waterman score: 336; 24.129 identity in 402 aa overlap ORF ftt0447c; hypothetical protein complement(462338..463678) Francisella tularensis subsp. tularensis SCHU S4 3191414 YP_169486.1 CDS glnS NC_006570.2 463699 465345 R catalyzes a two-step reaction, first charging a glutamine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; glutaminyl-tRNA synthetase complement(463699..465345) Francisella tularensis subsp. tularensis SCHU S4 3190997 YP_169487.1 CDS pckA NC_006570.2 465490 467082 D PEP carboxykinase; PEP carboxylase; PEPCK; catalyzes the phosphorylation and decarboxylation of oxaloacetate to form phosphoenolpyruvate using ATP; phosphoenolpyruvate carboxykinase 465490..467082 Francisella tularensis subsp. tularensis SCHU S4 3191431 YP_169488.1 CDS mraY NC_006570.2 467157 468254 D First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan; phospho-N-acetylmuramoyl-pentapeptide- transferase 467157..468254 Francisella tularensis subsp. tularensis SCHU S4 3191161 YP_169489.1 CDS murD NC_006570.2 468254 469504 D Similar to MURD_CHLCV (Q821S1) UDP-N-acetylmuramoylalanine--D-glutamate ligase from Chlamydophila caviae (419 aa) FASTA: opt: 516 Z-score: 615.2 E(): 2.2e-26 Smith-Waterman score: 549; 28.000 identity in 425 aa overlap; UDP-N-acetylmuramoylalanine--D-glutamate ligase 468254..469504 Francisella tularensis subsp. tularensis SCHU S4 3191954 YP_169490.1 CDS ftsW NC_006570.2 469514 470719 D Similar to Q9KPG6 Cell division protein FtsW from Vibrio cholerae (398 aa). FASTA: opt: 1114 Z-score: 1237.3 E(): 5e-61 Smith-Waterman score: 1114; 44.892 identity in 372 aa overlap; cell division protein FtsW 469514..470719 Francisella tularensis subsp. tularensis SCHU S4 3190868 YP_169491.1 CDS FTT_0453c NC_006570.2 470727 471533 R Similar to Q83DW2 Hypothetical protein from Coxiella burnetii (273 aa). FASTA: opt: 554 Z-score: 648.8 E(): 3e-28 Smith-Waterman score: 554; 37.407identity in 270 aa overlap ORF ftt0453c; hypothetical protein complement(470727..471533) Francisella tularensis subsp. tularensis SCHU S4 3191411 YP_169492.1 CDS yfdH NC_006570.2 471632 472588 D Similar to Q83DW4 Glycosyl transferase, group 2 family protein from Coxiella burnetii (324 aa). FASTA: opt: 896 Z-score: 1055.7 E(): 6.5e-51 Smith-Waterman score: 896; 43.189identity in 301 aa overlap; glycosyl transferase family protein 471632..472588 Francisella tularensis subsp. tularensis SCHU S4 3192164 YP_169493.1 CDS FTT_0455c NC_006570.2 472980 474743 R Similar to Q8XQM9 Probable transmembrane protein from Ralstonia solanacearum (551 aa). FASTA: opt: 683 Z-score: 749.4 E(): 7.6e-34 Smith-Waterman score: 683; 29.174 identity in 545 aa overlap ORF ftt0455c; dolichyl-phosphate-mannose-protein mannosyltransferase family protein complement(472980..474743) Francisella tularensis subsp. tularensis SCHU S4 3191408 YP_169494.1 CDS FTT_0456c NC_006570.2 474835 475128 R Similar to Y941_COXBU Hypothetical UPF0269 protein CBU0941 from Coxiella burnetii (90 aa). FASTA: opt: 346 Z-score: 470.4 E(): 2.6e-18 Smith-Waterman score: 346; 55.422 identity in 83 aa overlap ORF ftt0456c; hypothetical protein complement(474835..475128) Francisella tularensis subsp. tularensis SCHU S4 3191407 YP_169495.1 CDS yccK NC_006570.2 475119 475433 R Similar to Q9CNM6 Hypothetical protein PM0401 from Pasteurella multocida (109 aa). initn: 382 init1: 382 opt: 382 Z-score: 511.4 bits: 99.7 E(): 1.4e-20 Smith-Waterman score: 382; 58.586identity (58.58639ngapped) in 99 aa overlap; anaerobic sulfite reductase subunit complement(475119..475433) Francisella tularensis subsp. tularensis SCHU S4 3192160 YP_169496.1 CDS sspA NC_006570.2 475519 476151 D Similar to SSPA_HAESO (P31784) Stringent starvation protein A homolog from Haemophilus somnus (212 aa). FASTA: opt: 377 Z-score: 473.8 E(): 1.7e-18 Smith-Waterman score: 377; 35.106 identity in 188 aa overlap; stringent starvation protein A 475519..476151 Francisella tularensis subsp. tularensis SCHU S4 3190731 YP_169497.1 CDS sohB NC_006570.2 476163 477182 D SohB; periplasmic protein; member of the peptidase S49 family; periplasmic protease 476163..477182 Francisella tularensis subsp. tularensis SCHU S4 3190718 YP_169498.1 CDS holB NC_006570.2 477190 478101 D Similar to Q8D3A6 HolB protein from Wigglesworthia glossinidia brevipalpis (329 aa). FASTA: opt: 315 Z-score: 378.8 E(): 3.3e-13 Smith-Waterman score: 315; 27.304 identity in 293 aa overlap; DNA polymerase III subunit delta' 477190..478101 Francisella tularensis subsp. tularensis SCHU S4 3192338 YP_169499.1 CDS yhbY NC_006570.2 478112 478390 D Similar to Q87LZ3 RNA-binding protein containing KH domain from Vibrio parahaemolyticus (98 aa). FASTA: opt: 289 Z-score: 405.9 E(): 1e-14 Smith-Waterman score: 289; 52.222 identity in 90 aa overlap; RNA-binding protein 478112..478390 Francisella tularensis subsp. tularensis SCHU S4 3191267 YP_169500.1 CDS hemB NC_006570.2 478398 479372 D catalyzes the formation of porphobilinogen from 5-aminolevulinate; delta-aminolevulinic acid dehydratase 478398..479372 Francisella tularensis subsp. tularensis SCHU S4 3192415 YP_169501.1 CDS yibK NC_006570.2 479376 479843 D Similar to Q8E8X2 RNA methyltransferase, TrmH family, group 2 from Shewanella oneidensis (154 aa). FASTA: opt: 569 Z-score: 759.8 E(): 2e-34 Smith-Waterman score: 569; 57.616 identity in 151 aa overlap; tRNA/rRNA methyltransferase 479376..479843 Francisella tularensis subsp. tularensis SCHU S4 3191278 YP_169502.1 CDS ansB NC_006570.2 479961 481025 D Similar to ASG2_HAEIN (P43843) Probable L-asparaginase periplasmic [Precursor] from Haemophilus influenzae (349 aa). FASTA: opt: 897 Z-score: 1034.7 E(): 9.6e-50 Smith-Waterman score: 897; 43.966 identity in 348 aa overlap. No signal peptide predicted; periplasmic L-asparaginase II 479961..481025 Francisella tularensis subsp. tularensis SCHU S4 3192351 YP_169504.1 CDS argS NC_006570.2 481989 483734 R catalyzes a two-step reaction, first charging an arginine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA synthetase; arginyl-tRNA synthetase complement(481989..483734) Francisella tularensis subsp. tularensis SCHU S4 3190944 YP_169505.1 CDS ostA1 NC_006570.2 483860 486466 D Similar to OSTA_XYLFT (Q87AI9) Organic solvent tolerance protein from Xylella fastidiosa (792 aa). FASTA: opt: 700 Z-score: 808.7 E(): 3.7e-37 Smith-Waterman score: 775; 26.572 identity in 636 aa overlap; organic solvent tolerance protein 483860..486466 Francisella tularensis subsp. tularensis SCHU S4 3191215 YP_169506.1 CDS surA NC_006570.2 486448 487869 D Similar to Q8ZIK4 Survival protein SurA (Peptidyl-prolyl cis-trans isomerase) from Yersinia pestis (434 aa). FASTA: opt: 532 Z-score: 586.9 E(): 8.4e-25 Smith-Waterman score: 532; 25.176 identity in 425 aa overlap; peptidyl-prolyl cis-trans isomerase 486448..487869 Francisella tularensis subsp. tularensis SCHU S4 3190745 YP_169507.1 CDS ksgA NC_006570.2 487871 488659 D catalyzes the transfer of a total of four methyl groups from S-adenosyl-l-methionine (S-AdoMet) to two adjacent adenosine bases A1518 and A1519 in 16S rRNA; mutations in ksgA causes resistance to the translation initiation inhibitor kasugamycin; dimethyladenosine transferase 487871..488659 Francisella tularensis subsp. tularensis SCHU S4 3190742 YP_169508.1 CDS apaH NC_006570.2 488671 489498 D hydrolyzes P(1),P(4)-bis(5'-adenosyl) tetraphosphate to form 2 ADP; diadenosine tetraphosphatase 488671..489498 Francisella tularensis subsp. tularensis SCHU S4 3192350 YP_169509.1 CDS aroD NC_006570.2 489502 489939 D catalyzes the formation of 3-dehydroshikimate from 3-dehydroquinate in chorismate biosynthesis; 3-dehydroquinate dehydratase 489502..489939 Francisella tularensis subsp. tularensis SCHU S4 3192548 YP_169510.1 CDS accB NC_006570.2 489944 490417 D Similar to BCCP_PSEAE (P37799) Biotin carboxyl carrier protein of acetyl-CoA carboxylase from Pseudomonas aeruginosa (156 aa). FASTA: opt: 466 Z-score: 537.8 E(): 4.6e-22 Smith-Waterman score: 466; 48.148 identity in 162 aa overlap; acetyl-Coa carboxylase biotin carboxyl carrier protein subunit 489944..490417 Francisella tularensis subsp. tularensis SCHU S4 3191467 YP_169511.1 CDS accC NC_006570.2 490482 491837 D Similar to Q9KV62 Acetyl-CoA carboxylase, biotin carboxylase subunit from Vibrio cholerae (447 aa). FASTA: opt: 2119 Z-score: 2505.5 E(): 1.2e-131 Smith-Waterman score: 2119; 70.270 identity in 444 aa overlap; acetyl-Coa carboxylase biotin carboxylase subunit 490482..491837 Francisella tularensis subsp. tularensis SCHU S4 3192529 YP_169512.1 CDS FTT_0474 NC_006570.2 491931 492611 D ORF ftt0474; hypothetical protein 491931..492611 Francisella tularensis subsp. tularensis SCHU S4 3191405 YP_169513.1 CDS msc NC_006570.2 492637 493755 D Similar to Q87G16 (Q87G16) membrane protein from Vibrio parahaemolyticus (365 aa). FASTA: opt: 362 Z-score: 432.0 E(): 3.6e-16 Smith-Waterman score: 412; 29.167 identity in 384 aa overlap; mechanosensitive ion channel MscS 492637..493755 Francisella tularensis subsp. tularensis SCHU S4 3191637 YP_169514.1 CDS poxA NC_006570.2 493760 494683 R Similar to Q83AR3 (Q83AR3) Lysyl-tRNA synthetase-related protein from Coxiella burnetii (321 aa). FASTA: opt: 727 Z-score: 890.9 E(): 9.9e-42 Smith-Waterman score: 727; 42.088 identity in 297 aa overlap; lysyl-tRNA synthetase complement(493760..494683) Francisella tularensis subsp. tularensis SCHU S4 3191874 YP_169515.1 CDS birA NC_006570.2 494680 495462 R Similar to Q83CV0 BirA bifunctional protein from Coxiella burnetii (323 aa). FASTA: opt: 375 Z-score: 457.7 E(): 1.3e-17 Smith-Waterman score: 375; 31.004 identity in 229 aa overlap; transcription repressor BirA complement(494680..495462) Francisella tularensis subsp. tularensis SCHU S4 3190970 YP_169516.1 CDS recJ NC_006570.2 495459 497201 R Similar to RECJ_HAEIN (P45112) single-stranded-DNA-specific exonuclease from Haemophilus influenzae (575 aa). FASTA: opt: 869 Z-score: 996.6 E(): 1.3e-47 Smith-Waterman score: 902; 32.028identity in 562 aa overlap; single-stranded-DNA-specific exonuclease complement(495459..497201) Francisella tularensis subsp. tularensis SCHU S4 3191636 YP_169517.1 CDS perM NC_006570.2 497195 498301 R Similar to Q9I4W6 Hypothetical protein PA1007 from Pseudomonas aeruginosa (357 aa). FASTA: opt: 1016 Z-score: 1092.5 E(): 5.8e-53 Smith-Waterman score: 1016; 40.698 identity in 344 aa overlap; PerM family protein complement(497195..498301) Francisella tularensis subsp. tularensis SCHU S4 3191330 YP_169518.1 CDS xasA NC_006570.2 498403 499812 R Similar to Q8YBJ1 Glutamate:gamma-aminobutyrate antiporter from Brucella melitensis (510 aa). FASTA: opt: 983 Z-score: 1079.5 E(): 3.1e-52 Smith-Waterman score: 983; 34.698 identity in 464 aa overlap; glutamate:gamma-aminobutyric acid antiporter family protein complement(498403..499812) Francisella tularensis subsp. tularensis SCHU S4 3192117 YP_169519.1 CDS potF NC_006570.2 499896 501077 D Similar to Q92RX4 Probable putrescine-binding periplasmic protein from Rhizobium meliloti (364 aa). FASTA: opt: 847 Z-score: 993.2 E(): 2e-47 Smith-Waterman score: 847; 35.714 identity in 350 aa overlap; putrescine-binding periplasmic protein 499896..501077 Francisella tularensis subsp. tularensis SCHU S4 3191596 YP_169520.1 CDS FTT_0482c NC_006570.2 501330 502145 R ORF ftt0482c; lipoprotein complement(501330..502145) Francisella tularensis subsp. tularensis SCHU S4 3191404 YP_169521.1 CDS FTT_0483c NC_006570.2 502364 503251 R Similar to AAP95905 (AAP95905) Hypothetical protein from Haemophilus ducreyi 35000HP (342 aa). FASTA: opt: 686 Z-score: 815.5 E(): 1.6e-37 Smith-Waterman score: 686; 40.678 identity in 295 aa overlap ORF ftt0483c; hypothetical protein complement(502364..503251) Francisella tularensis subsp. tularensis SCHU S4 3191403 YP_169522.1 CDS FTT_0484 NC_006570.2 503405 504124 D ORF ftt0484; hypothetical protein 503405..504124 Francisella tularensis subsp. tularensis SCHU S4 3191402 YP_169523.1 CDS FTT_0485 NC_006570.2 504192 504854 D ORF ftt0485; hypothetical protein 504192..504854 Francisella tularensis subsp. tularensis SCHU S4 3191401 YP_169524.1 CDS mutL NC_006570.2 504862 506664 D Similar to MUTL_RICPR (Q9ZC88) DNA mismatch repair protein mutL from Rickettsia prowazekii (595 aa). FASTA: opt: 1236 Z-score: 1435.6 E(): 4.5e-72 Smith-Waterman score: 1236; 37.291 identity in 598 aa overlap; DNA mismatch repair protein 504862..506664 Francisella tularensis subsp. tularensis SCHU S4 3192051 YP_169526.1 CDS FTT_0488c NC_006570.2 507762 508895 R Similar to Q83BM0 Major facilitator family transporter from Coxiella burnetii (428 aa). FASTA: opt: 319 Z-score: E(): 3.7e-11 Smith-Waterman score: 319; 21.951 identity in 369 aa overlap ORF ftt0488c; major facilitator transporter complement(507762..508895) Francisella tularensis subsp. tularensis SCHU S4 3191397 YP_169527.1 CDS trxB NC_006570.2 509037 509987 R Similar to Q8ZGC9 Thioredoxin reductase from Yersinia pestis (320 aa). FASTA: opt: 1525 Z-score: 1798.2 E(): 2.9e-92 Smith-Waterman score: 1525; 70.927 identity in 313 aa overlap; thioredoxin reductase complement(509037..509987) Francisella tularensis subsp. tularensis SCHU S4 3191000 YP_169528.1 CDS FTT_0490c NC_006570.2 510079 511296 R Similar Q888K0 Phospholipase D family protein from Pseudomonas syringae (pv. tomato) (422 aa). FASTA: opt: 440 Z-score: 514.4 E(): 9.2e-21 Smith-Waterman score: 440; 25.840 identity in 387 aa overlap ORF ftt0490c; phospholipase D complement(510079..511296) Francisella tularensis subsp. tularensis SCHU S4 3191396 YP_169529.1 CDS gph NC_006570.2 511339 512013 R Similar to Q82SZ4 Phosphoglycolate phosphatase from Nitrosomonas europaea (240 aa). FASTA: opt: 432 Z-score: 532.2 E(): 9.4e-22 Smith-Waterman score: 432; 33.486 identity in 218 aa overlap; phosphoglycolate phosphatase complement(511339..512013) Francisella tularensis subsp. tularensis SCHU S4 3192528 YP_169530.1 CDS lysR NC_006570.2 512054 512980 R Similar to Q87J66 Transcriptional regulator, LysR family from Vibrio parahaemolyticus (311 aa). FASTA: opt: 675 Z-score: 768.6 E(): 6.4e-35 Smith-Waterman score: 675; 33.115 identity in 305 aa overlap; LysR family transcriptional regulator complement(512054..512980) Francisella tularensis subsp. tularensis SCHU S4 3190932 YP_169531.1 CDS cutC NC_006570.2 514279 515001 R Similar Q9CNA6 Hypothetical protein PM0526 from Pasteurella multocida (244 aa). FASTA: opt: 521 Z-score: 651.2 E(): 2.2e-28 Smith-Waterman score: 521; 40.175 identity in 229 aa overlap; CutC family protein complement(514279..515001) Francisella tularensis subsp. tularensis SCHU S4 3191903 YP_169532.1 CDS FTT_0495 NC_006570.2 515334 515837 D Similar to Q9K0Q8 Hypothetical protein NMB0526 from Neisseria meningitidis (serogroup B) (172 aa). FASTA: opt: 455 Z-score: 573.9 E(): 4.5e-24 Smith-Waterman score: 455; 42.353 identity in 170 aa overlap ORF ftt0495; hypothetical protein 515334..515837 Francisella tularensis subsp. tularensis SCHU S4 3191393 YP_169533.1 CDS FTT_0496 NC_006570.2 515843 516607 D Similar to Q935B8 Hypothetical protein HCM2.0044c from Salmonella typhi (251 aa). FASTA: opt: 242 Z-score: 297.7 E(): 1.1e-08 Smith-Waterman score: 296; 28.000 identity in 250 aa overlap ORF ftt0496; hypothetical protein 515843..516607 Francisella tularensis subsp. tularensis SCHU S4 3191392 YP_169536.1 CDS FTT_0501c NC_006570.2 520542 521582 R Similar to Q9I0I7 Hypothetical protein PA2651 from Pseudomonas aeruginosa (352 aa). FASTA: opt: 382 Z-score: 397.1 E(): 3.2e-14 Smith-Waterman score: 382; 26.686 identity in 341 aa overlap ORF ftt0501c; hypothetical protein complement(520542..521582) Francisella tularensis subsp. tularensis SCHU S4 3191386 YP_169537.1 CDS FTT_0502c NC_006570.2 521966 522391 R ORF ftt0502c; hypothetical protein complement(521966..522391) Francisella tularensis subsp. tularensis SCHU S4 3191385 YP_169538.1 CDS sucD NC_006570.2 522503 523375 R Similar to Q9JUS9 succinyl-CoA synthetase alpha from Neisseria meningitidis (serogroup A) (296 aa). FASTA: opt: 1574 Z-score: 1817.5 E(): 2.4e-93 Smith-Waterman score: 1574; 81.661identity in 289 aa overlap; succinyl-CoA synthetase subunit alpha complement(522503..523375) Francisella tularensis subsp. tularensis SCHU S4 3190740 YP_169539.1 CDS sucC NC_006570.2 523407 524570 R catalyzes the interconversion of succinyl-CoA and succinate; succinyl-CoA synthetase subunit beta complement(523407..524570) Francisella tularensis subsp. tularensis SCHU S4 3190738 YP_169540.1 CDS FTT_0505 NC_006570.2 524740 526653 D ORF ftt0505; hypothetical protein 524740..526653 Francisella tularensis subsp. tularensis SCHU S4 3191381 YP_169541.1 CDS FTT_0506c NC_006570.2 526661 526975 R ORF ftt0506c; hypothetical protein complement(526661..526975) Francisella tularensis subsp. tularensis SCHU S4 3191384 YP_169542.1 CDS FTT_0507 NC_006570.2 527634 528401 D Similar to Q9X610 BcfH from Salmonella typhimurium (269 aa). FASTA: opt: 305 Z-score: 371.4 E(): 8.5e-13 Smith-Waterman score: 305; 31.122 identity in 196 aa overlap ORF ftt0507; lipoprotein 527634..528401 Francisella tularensis subsp. tularensis SCHU S4 3191382 YP_169543.1 CDS dusA NC_006570.2 528408 529391 R Similar to Y926_SYNY3 (P72872) Hypothetical protein sll0926 from Synechocystis sp. (strain PCC 6803) (334 aa). FASTA: opt: 1129 Z-score: 1337.0 E(): 1.4e-66 Smith-Waterman score: 1129; 51.911 identity in 314 aa overlap; tRNA-dihydrouridine synthase A complement(528408..529391) Francisella tularensis subsp. tularensis SCHU S4 3191548 YP_169544.1 CDS FTT_0509c NC_006570.2 529425 530225 R Similar to Q8DBN3 Conserved hypothetical protein from Vibrio vulnificus (184 aa). FASTA: opt: 275 Z-score: 356.8 E(): 5.5e-12 Smith-Waterman score: 275; 30.208 identity in 192 aa overlap ORF ftt0509c; hypothetical protein complement(529425..530225) Francisella tularensis subsp. tularensis SCHU S4 3191380 YP_169545.1 CDS gyrB NC_006570.2 530401 532818 D Similar to GYRB_SALTY (Q60008) DNA gyrase subunit B from Salmonella typhimurium (803 aa). FASTA: opt: 3370 Z-score: 3873.3 E(): 7.5e-208 Smith-Waterman score: 3370; 63.321 identity in 807 aa overlap; DNA gyrase subunit B 530401..532818 Francisella tularensis subsp. tularensis SCHU S4 3192346 YP_169546.1 CDS FTT_0511 NC_006570.2 532948 533811 D with PdxT forms pyridoxal 5'-phosphate from glutamine, either ribose 5-phosphate or ribulose 5-phosphate, and either glyceraldehyde 3-phosphate or dihydroxyacetone phosphate; pyridoxal biosynthesis lyase PdxS 532948..533811 Francisella tularensis subsp. tularensis SCHU S4 3191379 YP_169547.3 CDS FTT_0512 NC_006570.2 533805 534353 D with PdxST is involved in the biosynthesis of pyridoxal 5'-phosphate; PdxT catalyzes the hydrolysis of glutamine to glutamate and ammonia; PdxS utilizes the ammonia to synthesize pyridoxal 5'-phosphate; glutamine amidotransferase subunit PdxT 533805..534353 Francisella tularensis subsp. tularensis SCHU S4 3191376 YP_169548.1 CDS isftu1 NC_006570.2 534457 535241 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(534457..534915,534915..535241)) Francisella tularensis subsp. tularensis SCHU S4 3190798 YP_169549.1 CDS FTT_0515 NC_006570.2 536458 536634 D ORF ftt0515; hypothetical protein 536458..536634 Francisella tularensis subsp. tularensis SCHU S4 3191377 YP_169550.1 CDS prmA NC_006570.2 541312 542157 D Similar to Q9CLW2 PrmA (Ribosomal protein L11 methyltransferase) from Pasteurella multocida (293 aa). FASTA: opt: 572 Z-score: 685.5 E(): 2.7e-30 Smith-Waterman score: 580; 33.677 identity in 291 aa overlap; 50S ribosomal protein L11 methyltransferase 541312..542157 Francisella tularensis subsp. tularensis SCHU S4 3191234 YP_169551.1 CDS FTT_0519 NC_006570.2 542147 543130 D Similar to Q9KGG5 Transcriptional regulator involved in nitrogen regulation, NifR3/Smm1 family from Bacillus halodurans (334 aa). FASTA: opt: 1134 Z-score: 1336.1 E(): 1.6e-66 Smith-Waterman score: 1134; 52.484 identity in 322 aa overlap ORF ftt0519; Nif3 family protein 542147..543130 Francisella tularensis subsp. tularensis SCHU S4 3191226 YP_169554.1 CDS FTT_0523 NC_006570.2 545582 546808 D Similar to Q89Z57 Type I restriction enzyme EcoAI specificity protein from Bacteroides thetaiotamicron (474 aa). FASTA: opt: 208 Z-score: 239.0 E(): 2e-05 Smith-Waterman score: 246; 24.363 identity in 353 aa overlap ORF ftt0523; hypothetical protein 545582..546808 Francisella tularensis subsp. tularensis SCHU S4 3191219 YP_169555.1 CDS FTT_0524 NC_006570.2 547050 547436 D Similar to Q8KBB0 Hypothetical protein CT1879 from Chlorobium tepidum (193 aa). FASTA: opt: 467 Z-score: 629.2 E(): 3.7e-27 Smith-Waterman score: 467; 60.656 identity in 122 aa overlap ORF ftt0524; hypothetical protein 547050..547436 Francisella tularensis subsp. tularensis SCHU S4 3191220 YP_169556.1 CDS FTT_0525 NC_006570.2 547483 548139 D Similar to RA50_THEVO DNA double-strand break repair rad50 ATPase from Thermoplasma volcanicum (895 aa). FASTA: opt: 188 Z-score: 215.6 E(): 0.0004 Smith-Waterman score: 188; 25.926 identity in 216 aa overlap. Similarity to N terminal domain ORF ftt0525; hypothetical protein 547483..548139 Francisella tularensis subsp. tularensis SCHU S4 3191218 YP_169557.1 CDS FTT_0526 NC_006570.2 548145 548351 D ORF ftt0526; hypothetical protein 548145..548351 Francisella tularensis subsp. tularensis SCHU S4 3191216 YP_169560.1 CDS FTT_0530c NC_006570.2 550624 551226 R Similar to Q97FB4 Intracellular protease/amidase related enzyme from Clostridium acetobutylicum (201 aa). FASTA: opt: 703 Z-score: 884.5 E(): 2.2e-41 Smith-Waterman score: 703; 51.531 identity in 196 aa overlap ORF ftt0530c; DJ-1/PfpI family protein complement(550624..551226) Francisella tularensis subsp. tularensis SCHU S4 3191211 YP_169561.1 CDS grxC NC_006570.2 553228 554460 R Similar to (glutaredoxin 3) Q8ZJM8 Glutaredoxin 3 from Yersinia pestis (82 aa). FASTA: opt: 181 Z-score: 263.7 E(): 8.5e-07 Smith-Waterman score: 181; 40.260 identity in 77 aa overlap,(ribonucleoside-diphosphate reductase) Q8QYZ8 Ribonucleoside-diphosphate reductase, beta subunit-like protein from Rana tigrina ranavirus (387 aa). FASTA: opt: 1124 Z-score: 1382.6 E(): 4.1e-69 Smith-Waterman score: 1124; 51.703 identity in 323 aa overlap; bifunctional gluaredoxin/ribonucleoside-diphosphate reductase subunit beta complement(553228..554460) Francisella tularensis subsp. tularensis SCHU S4 3191711 YP_169562.1 CDS grxA NC_006570.2 554467 554727 R Similar to GLR1_ECOLI (P00277) Glutaredoxin 1 from Escherichia coli (85 aa). FASTA: opt: 191 Z-score: 274.9 E(): 2e-07 Smith-Waterman score: 191; 40.000identity in 80 aa overlap; glutaredoxin complement(554467..554727) Francisella tularensis subsp. tularensis SCHU S4 3192520 YP_169563.1 CDS nrdA NC_006570.2 554731 556503 R Catalyzes the rate-limiting step in dNTP synthesis; ribonucleotide-diphosphate reductase subunit alpha complement(554731..556503) Francisella tularensis subsp. tularensis SCHU S4 3191708 YP_169564.1 CDS mdh NC_006570.2 556707 557666 R Catalyzes the reversible oxidation of malate to oxaloacetate; malate dehydrogenase complement(556707..557666) Francisella tularensis subsp. tularensis SCHU S4 3191869 YP_169565.1 CDS FTT_0536 NC_006570.2 557814 558329 D ORF ftt0536; hypothetical protein 557814..558329 Francisella tularensis subsp. tularensis SCHU S4 3191210 YP_169566.1 CDS FTT_0537 NC_006570.2 558345 558980 D Similar to Q8PD67 Ubiquinone biosynthesis protein from Xanthomonas campestris (217 aa). FASTA: opt: 710 Z-score: 878.4 E(): 4.9e-41 Smith-Waterman score: 710; 51.887 identity in 212 aa overlap (2-211:8-217) ORF ftt0537; ubiquinone biosynthesis protein 558345..558980 Francisella tularensis subsp. tularensis SCHU S4 3191209 YP_169567.1 CDS FTT_0538c NC_006570.2 558989 559558 R Similar to Q87LS3 Hypothetical protein from Vibrio parahaemolyticus (203 aa). FASTA: opt: 267 Z-score: 350.1 E(): 1.3e-11 Smith-Waterman score: 320; 31.795 identity in 195 aa overlap ORF ftt0538c; lipoprotein complement(558989..559558) Francisella tularensis subsp. tularensis SCHU S4 3191206 YP_169568.1 CDS FTT_0540c NC_006570.2 560383 561873 R ORF ftt0540c; hypothetical protein complement(560383..561873) Francisella tularensis subsp. tularensis SCHU S4 3191205 YP_169569.1 CDS yqaB NC_006570.2 561928 562527 R Similar to YQAB_ECOLI (P77475) Hypothetical protein yqaB from Escherichia coli (188 aa). FASTA: opt: 336 Z-score: 422.2 E(): 1.3e-15 Smith-Waterman score: 336; 33.889 identity in 180 aa overlap; haloacid dehalogenase complement(561928..562527) Francisella tularensis subsp. tularensis SCHU S4 3192402 YP_169570.1 CDS FTT_0543 NC_006570.2 563268 564644 D ORF ftt0543; hypothetical protein 563268..564644 Francisella tularensis subsp. tularensis SCHU S4 3191204 YP_169571.1 CDS phnA NC_006570.2 564750 565082 D Similar to PHNA_ECOLI PhnA protein from Escherichia coli (111 aa). FASTA: opt: 521 Z-score: 674.6 E(): 1.1e-29 Smith-Waterman score: 521; 66.972identity in 109 aa overlap; phosphonoacetate hydrolase 564750..565082 Francisella tularensis subsp. tularensis SCHU S4 3191136 YP_169572.1 CDS FTT_0545 NC_006570.2 565088 565303 D ORF ftt0545; hypothetical protein 565088..565303 Francisella tularensis subsp. tularensis SCHU S4 3191203 YP_169573.1 CDS FTT_0546 NC_006570.2 565307 565918 D ORF ftt0546; hypothetical protein 565307..565918 Francisella tularensis subsp. tularensis SCHU S4 3191202 YP_169575.1 CDS dnaQ NC_006570.2 566858 567550 D Similar to Q83EK2 DNA polymerase III, epsilon subunit from Coxiella burnetii (232 aa). FASTA: opt: 699 Z-score: 870.8 E(): 1.3e-40 Smith-Waterman score: 699; 49.550 identity in 222 aa overlap; DNA polymerase III subunit epsilon 566858..567550 Francisella tularensis subsp. tularensis SCHU S4 3191535 YP_169576.1 CDS vanY NC_006570.2 567544 568017 D Similar to Q87AM4 Peptidase (202 aa). FASTA: opt: 481 Z-score: 603.7 E(): 9.8e-26 Smith-Waterman score: 481; 50.000 identity in 142 aa overlap; D-alanyl-D-alanine carboxypeptidase 567544..568017 Francisella tularensis subsp. tularensis SCHU S4 3191938 YP_169577.1 CDS FTT_0550 NC_006570.2 567977 569413 D Similar to Q8EHZ3 TPR domain protein from Shewanella oneidensis (466 aa). FASTA: opt: 304 Z-score: 346.2 E(): 2.2e-11 Smith-Waterman score: 331; 23.333 identity) in 480 aa overlap ORF ftt0550; hypothetical protein 567977..569413 Francisella tularensis subsp. tularensis SCHU S4 3191200 YP_169578.1 CDS FTT_0552 NC_006570.2 570846 572342 D Similar to Q8P9Q7 Aldehyde dehydrogenase from Xanthomonas campestris (510 aa). FASTA: opt: 1972 Z-score: 2329.9 E(): 6.9e-122 Smith-Waterman score: 1972; 60.285 identity in 491 aa overlap ORF ftt0552; aldehyde dehydrogenase 570846..572342 Francisella tularensis subsp. tularensis SCHU S4 3191197 YP_169579.1 CDS FTT_0553 NC_006570.2 572347 573126 D Similar to Q8EJ53 Conserved hypothetical protein from Shewanella oneidensis (267 aa). FASTA: opt: 859 Z-score: 1057.9 E(): 4.9e-51 Smith-Waterman score: 859; 52.809 identity in 267 aa overlap ORF ftt0553; hypothetical protein 572347..573126 Francisella tularensis subsp. tularensis SCHU S4 3191762 YP_169580.1 CDS FTT_0554 NC_006570.2 573210 573482 D Similar to Q99SH0 Hypothetical protein SAV2087 from Stafylococcus aureus (97 aa). FASTA: opt: 243 Z-score: 331.6 E(): 1.4e-10 Smith-Waterman score: 243; 45.455 identity in 77 aa overlap ORF ftt0554; hypothetical protein 573210..573482 Francisella tularensis subsp. tularensis SCHU S4 3191196 YP_169581.1 CDS FTT_0555 NC_006570.2 573496 574227 D Similar to Q930P5 Hypothetical protein RA0150 from Rhizobium meliloti (262 aa). FASTA: opt: 924 Z-score: 1048.2 E(): 1.7e-50 Smith-Waterman score: 924; 54.202 identity in 238 aa overlap. Some similarity to glutaredoxin ORF ftt0555; hypothetical protein 573496..574227 Francisella tularensis subsp. tularensis SCHU S4 3191193 YP_169582.1 CDS oxyR NC_006570.2 574230 575099 R Similar to O06466 Oxidative stress transcriptional regulator from Xanthomonas campestris (313 aa). FASTA: opt: 707 Z-score: 842.2 E(): 5.1e-39 Smith-Waterman score: 707; 36.207 identity in 290 aa overlap; oxidative stress transcriptional regulator complement(574230..575099) Francisella tularensis subsp. tularensis SCHU S4 3191225 YP_169583.1 CDS FTT_0557 NC_006570.2 575197 575721 D Similar to Q92MY1 Hypothetical protein R02467 from Rhizobium meliloti (180 aa). FASTA: opt: 821 Z-score: 1055.0 E(): 7.2e-51 Smith-Waterman score: 821; 66.092 identity in 174 aa overlap ORF ftt0557; AhpC/TSA family protein 575197..575721 Francisella tularensis subsp. tularensis SCHU S4 3191195 YP_169584.1 CDS FTT_0558 NC_006570.2 575724 576335 D Similar to Q8XU62 Hypothetical protein RSc3331 from Ralstonia solanacearum (201 aa). FASTA: opt: 602 Z-score: 724.0 E(): 2e-32 Smith-Waterman score: 602; 48.020 identity in 202 aa overlap ORF ftt0558; short chain dehydrogenase 575724..576335 Francisella tularensis subsp. tularensis SCHU S4 3191194 YP_169585.1 CDS cmk NC_006570.2 576352 577017 R Similar to KCY_PASMU (P57875) Cytidylate kinase from Pasteurella multocida (227 aa). FASTA: opt: 748 Z-score: 879.9 E(): 4.1e-41 Smith-Waterman score: 748; 53.425 identity in 219 aa overlap; cytidylate kinase complement(576352..577017) Francisella tularensis subsp. tularensis SCHU S4 3192510 YP_169586.1 CDS serC NC_006570.2 577004 578056 R catalyzes the formation of 3-phosphonooxypyruvate and glutamate from O-phospho-L-serine and 2-oxoglutarate; required both in major phosphorylated pathway of serine biosynthesis and in the biosynthesis of pyridoxine; phosphoserine aminotransferase complement(577004..578056) Francisella tularensis subsp. tularensis SCHU S4 3190960 YP_169587.1 CDS isftu1 NC_006570.2 578376 579160 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(578376..578702,578702..579160) Francisella tularensis subsp. tularensis SCHU S4 3190912 YP_169588.1 CDS potG NC_006570.2 579542 580663 D Similar to Q8EHF2 Polyamine ABC transporter, ATP-binding protein from Shewanella oneidensis (378 aa). FASTA: opt: 1366 Z-score: 1557.5 E(): 7.3e-79 Smith-Waterman score: 1366; 55.496 identity in 373 aa overlap; polyamine transporter ABC transporter ATP-binding protein 579542..580663 Francisella tularensis subsp. tularensis SCHU S4 3191563 YP_169589.1 CDS potH NC_006570.2 580627 581544 D Similar to Q8FJE8 Putrescine transport system permease protein from Escherichia coli (314 aa). FASTA: opt: 949 Z-score: 1040.4 E(): 4.7e-50 Smith-Waterman score: 949; 43.878 identity in 294 aa overlap; polyamine transporter ABC transporter membrane protein 580627..581544 Francisella tularensis subsp. tularensis SCHU S4 3190974 YP_169590.1 CDS potI NC_006570.2 581558 582364 D Similar to Q8EHF0 Polyamine ABC transporter, permease protein from Shewanella oneidensis (271 aa). FASTA: opt: 873 Z-score: 1010.7 E(): 2.1e-48 Smith-Waterman score: 873; 46.241 identity in 266 aa overlap; polyamine transporter ABC transporter membrane protein 581558..582364 Francisella tularensis subsp. tularensis SCHU S4 3191478 YP_169591.1 CDS isftu2 NC_006570.2 582508 583251 R ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 transposase from Francisella tularensis (247 aa).; transposase complement(582508..583251) Francisella tularensis subsp. tularensis SCHU S4 3190925 YP_169592.1 CDS FTT_0566 NC_006570.2 583397 583663 D ORF ftt0566; hypothetical protein 583397..583663 Francisella tularensis subsp. tularensis SCHU S4 3191192 YP_169593.1 CDS FTT_0568 NC_006570.2 585869 586519 D Similar to Q26545 Phosphoserine phosphohydrolase-like protein trans-spliced from Schistosoma mansoni (223 aa). FASTA: opt: 319 Z-score: 380.8 E(): 2.5e-13 Smith-Waterman score: 319; 29.493 identity in 217 aa overlap. Similar to eukaryotic proteins. Historical HGT-event? ORF ftt0568; hypothetical protein 585869..586519 Francisella tularensis subsp. tularensis SCHU S4 3191190 YP_169594.1 CDS FTT_0569c NC_006570.2 586508 587530 R Similar to Q8P3T2 Integral membrane protein from Xanthomonas campestris (347 aa). FASTA: opt: 906 Z-score: 1071.1 E(): 9e-52 Smith-Waterman score: 906; 41.739 identity in 345 aa overlap ORF ftt0569c; hypothetical protein complement(586508..587530) Francisella tularensis subsp. tularensis SCHU S4 3191188 YP_169595.1 CDS FTT_0570 NC_006570.2 587610 588008 D ORF ftt0570; hypothetical protein 587610..588008 Francisella tularensis subsp. tularensis SCHU S4 3191186 YP_169596.1 CDS FTT_0571 NC_006570.2 588020 588727 D Partially similar to Q96F61 Hypothetical protein from Homo sapiens (409 aa). FASTA: opt: 403 Z-score: 516.3 E(): 7.3e-21 Smith-Waterman score: 403; 34.171 identity in 199 aa overlap ORF ftt0571; hypothetical protein 588020..588727 Francisella tularensis subsp. tularensis SCHU S4 3191185 YP_169597.1 CDS FTT_0572 NC_006570.2 588852 590306 D Similar to Q8Z269 PTR2 family transport protein from Salmonella typhi (489 aa). FASTA: opt: 729 Z-score: 778.1 E(): 1.9e-35 Smith-Waterman score: 770; 30.524identity in 439 aa overlap ORF ftt0572; proton-dependent oligopeptide transport (POT) family protein 588852..590306 Francisella tularensis subsp. tularensis SCHU S4 3191184 YP_169598.1 CDS alr NC_006570.2 590357 591454 D Similar to Q81EB9 Alanine racemase from Bacillus cereus (408 aa). FASTA: opt: 573 Z-score: 679.1 E(): 6.2e-30 Smith-Waterman score: 573; 29.863 identity in 365 aa overlap; alanine racemase 590357..591454 Francisella tularensis subsp. tularensis SCHU S4 3192353 YP_169599.1 CDS pheA NC_006570.2 593229 594071 D Similar to Q8KBW6 Prephenate dehydratase from chlorobium tepidum (280 aa). FASTA: opt: 624 Z-score: 792.7 E(): 2.9e-36 Smith-Waterman score: 624; 38.489 identity in 278 aa overlap; prephenate dehydratase 593229..594071 Francisella tularensis subsp. tularensis SCHU S4 3191368 YP_169600.1 CDS FTT_0576 NC_006570.2 594097 594765 D Similar to Q894N2 zinc metallopeptidase from Clostridium tetani (236 aa). FASTA: opt: 434 Z-score: 526.0 E(): 2.1e-21 Smith-Waterman score: 434; 36.245 identity in 229 aa overlap ORF ftt0576; hypothetical protein 594097..594765 Francisella tularensis subsp. tularensis SCHU S4 3191182 YP_169601.1 CDS sdaA NC_006570.2 594821 596194 D Similar to Q8EEW4 L-serine dehydratase 1 from Shewanella oneidensis (458 aa). FASTA: opt: 1442 Z-score: 1806.4 E(): 1e-92 Smith-Waterman score: 1442; 48.922 identity in 464 aa overlap; L-serine dehydratase 1 594821..596194 Francisella tularensis subsp. tularensis SCHU S4 3192275 YP_169602.1 CDS csdB NC_006570.2 596297 597520 D Similar to Q8ZDZ4 selenocysteine lyase from Yersinia pestis (406 aa). FASTA: opt: 1343 Z-score: 1645.0 E(): 9.9e-84 Smith-Waterman score: 1343; 48.507 identity in 402 aa overlap; selenocysteine lyase 596297..597520 Francisella tularensis subsp. tularensis SCHU S4 3191895 YP_169603.1 CDS FTT_0579 NC_006570.2 597541 597897 D Similar to AAP95950 HesB family protein from Haemophilus ducreyi (107 aa). FASTA: opt: 288 Z-score: 392.8 E(): 5.5e-14 Smith-Waterman score: 288; 41.121 identity in 107 aa overlap ORF ftt0579; HesB family protein 597541..597897 Francisella tularensis subsp. tularensis SCHU S4 3191180 YP_169604.1 CDS FTT_0580 NC_006570.2 597911 598462 D Similar to Q83BY3 Hypothetical protein from Coxiella burnetii (180 aa). FASTA: opt: 593 Z-score: 750.6 E(): 6.4e-34 Smith-Waterman score: 593; 48.045 identity in 179 aa overlap ORF ftt0580; hypothetical protein 597911..598462 Francisella tularensis subsp. tularensis SCHU S4 3191179 YP_169605.1 CDS coaD NC_006570.2 598474 598962 D Catalyzes the conversion of ATP and pantetheine 4'-phosphate to diphosphate and 3'-dephospho-coA; phosphopantetheine adenylyltransferase 598474..598962 Francisella tularensis subsp. tularensis SCHU S4 3192516 YP_169606.1 CDS fdx NC_006570.2 598967 599212 D ferredoxin. Identical to Q47937 Ferredoxin from Francisella novicida. Similar to Q88AH4 Ferredoxin from pseudomonas syringae (83 aa). FASTA: opt: 431 Z-score: 563.2 E(): 1.8e-23 Smith-Waterman score: 431; 65.432 identity in 81 aa overlap; ferredoxin 598967..599212 Francisella tularensis subsp. tularensis SCHU S4 3191881 YP_169607.1 CDS fopA1 NC_006570.2 599319 600500 D No transmembrane helix predicted.; outer membrane associated protein 599319..600500 Francisella tularensis subsp. tularensis SCHU S4 3192005 YP_169608.1 CDS FTT_0584 NC_006570.2 601162 604533 D ORF ftt0584; hypothetical protein 601162..604533 Francisella tularensis subsp. tularensis SCHU S4 3191177 YP_169609.1 CDS FTT_0586 NC_006570.2 605902 606480 D Similar to Q9YCL9 Hypothetical protein APE1239 from Aeropyrum pernix (221 aa). FASTA: opt: 238 Z-score: 303.8 E(): 5e-09 Smith-Waterman score: 238; 25.683 identity in 183 aa overlap. Weakly conserved. ORF ftt0586; hypothetical protein 605902..606480 Francisella tularensis subsp. tularensis SCHU S4 3191175 YP_169610.1 CDS isftu2 NC_006570.2 606904 607647 R ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 transposase from Francisella tularensis (247 aa).; transposase complement(606904..607647) Francisella tularensis subsp. tularensis SCHU S4 3190840 YP_169611.1 CDS aroA NC_006570.2 607810 609087 D Similar to AROA_VIBPA (Q87QX9) 3-phosphoshikimate 1-carboxyvinyltransferase from Vibrio parahaemolyticus (426 aa). FASTA: opt: 928 Z-score: 1157.4 E(): 1.4e-56 Smith-Waterman score: 928; 36.916 identity in 428 aa overlap; 3-phosphoshikimate 1-carboxyvinyltransferase 607810..609087 Francisella tularensis subsp. tularensis SCHU S4 3191078 YP_169612.1 CDS FTT_0589 NC_006570.2 609090 609680 D ORF ftt0589; hypothetical protein 609090..609680 Francisella tularensis subsp. tularensis SCHU S4 3191174 YP_169613.1 CDS rnhA NC_006570.2 609631 610113 D Similar to RNH_PSEAE (Q9I2S9) Ribonuclease H from Pseudomonas aeruginosa (148 aa). FASTA: opt: 673 Z-score: 830.4 E(): 2.3e-38 Smith-Waterman score: 673; 62.937 identity in 143 aa overlap; ribonuclease H 609631..610113 Francisella tularensis subsp. tularensis SCHU S4 3191605 YP_169614.1 CDS ansA NC_006570.2 610115 611152 D Similar to Q8EEG1 L-asparaginase I from Shewanella oneidensis (337 aa). FASTA: opt: 1041 Z-score: 1293.4 E(): 3.7e-64 Smith-Waterman score: 1041; 49.258identity in 337 aa overlap; L-asparaginase 610115..611152 Francisella tularensis subsp. tularensis SCHU S4 3192357 YP_169615.1 CDS cynT NC_006570.2 611223 611909 D Similar to Q87AM3 Carbonic anhydrase from xylella fastidiosa (220 aa). FASTA: opt: 577 Z-score: 733.9 E(): 5.5e-33 Smith-Waterman score: 722; 45.536 identity in 224 aa overlap; carbonic anhydrase 611223..611909 Francisella tularensis subsp. tularensis SCHU S4 3191916 YP_169616.1 CDS FTT_0593c NC_006570.2 612196 612801 R ORF ftt0593c; hypothetical protein complement(612196..612801) Francisella tularensis subsp. tularensis SCHU S4 3191172 YP_169617.1 CDS FTT_0594c NC_006570.2 612865 613737 R Similar to Q87N89 Hypothetical protein from Vibrio parahaemolyticus (344 aa). FASTA: opt: 706 Z-score: 859.6 E(): 5.5e-40 Smith-Waterman score: 706; 42.160 identity in 287 aa overlap ORF ftt0594c; hypothetical protein complement(612865..613737) Francisella tularensis subsp. tularensis SCHU S4 3191171 YP_169618.1 CDS rubA NC_006570.2 613746 613916 R Similar to Q88C68 Rubredoxin from Pseudomonas putida (55 aa). FASTA: opt: 334 Z-score: 465.9 E(): 4.6e-18 Smith-Waterman score: 334; 78.431 identity in 51 aa overlap; rubredoxin complement(613746..613916) Francisella tularensis subsp. tularensis SCHU S4 3191088 YP_169619.1 CDS FTT_0596c NC_006570.2 614003 614620 R ORF ftt0596c; hypothetical protein complement(614003..614620) Francisella tularensis subsp. tularensis SCHU S4 3191170 YP_169620.1 CDS FTT_0597 NC_006570.2 614732 616126 D Similar to Q9K5Z6 Hypothetical UPF0061 protein BH393 from Bacillus halodurans (492 aa). FASTA: opt: 1251 Z-score: 1519.2 E(): 1e-76 Smith-Waterman score: 1357; 48.233 identity in 481 aa overlap ORF ftt0597; hypothetical protein 614732..616126 Francisella tularensis subsp. tularensis SCHU S4 3191169 YP_169621.1 CDS FTT_0598c NC_006570.2 616158 617438 R Similar to Q8XLU3 Probable transmembrane symporter from Clostridium perfringens (435 aa). FASTA: opt: 881 Z-score: 961.9 E(): 1.1e-45 Smith-Waterman score: 881; 33.721identity in 430 aa overlap ORF ftt0598c; sodium-dicarboxylate symporter family protein complement(616158..617438) Francisella tularensis subsp. tularensis SCHU S4 3191168 YP_169622.1 CDS FTT_0599 NC_006570.2 617634 618662 D Similar to Q9AAR1 SIS domain protein from Caulobacter crescentus (358 aa). FASTA: opt: 864 Z-score: 1052.1 E(): 1e-50 Smith-Waterman score: 864; 42.353 identity in 340 aa overlap. Glucosamine-fructose-6-phosphate aminotransferase ORF ftt0599; SIS domain-containing protein 617634..618662 Francisella tularensis subsp. tularensis SCHU S4 3191165 YP_169623.1 CDS FTT_0601 NC_006570.2 620247 620366 D ORF ftt0601; hypothetical protein 620247..620366 Francisella tularensis subsp. tularensis SCHU S4 3191164 YP_169624.1 CDS FTT_0602c NC_006570.2 620993 622471 R ORF ftt0602c; hypothetical protein complement(620993..622471) Francisella tularensis subsp. tularensis SCHU S4 3191163 YP_169625.1 CDS FTT_0603 NC_006570.2 623031 623210 D ORF ftt0603; hypothetical protein 623031..623210 Francisella tularensis subsp. tularensis SCHU S4 3191162 YP_169626.1 CDS FTT_0604 NC_006570.2 623728 624162 D Similar to Q9CNR2 NhaP NA+/H+ antiporter Conserved hypothetical membrane protein from Pasteurella multocida (441 aa). FASTA: opt: 315 Z-score: 391.3 E(): 6.6e-14 Smith-Waterman score: 315; 39.850 identity in 133 aa overlap. Matches to a central part of an Nhap protein ORF ftt0604; hypothetical protein 623728..624162 Francisella tularensis subsp. tularensis SCHU S4 3191159 YP_169627.1 CDS FTT_0605c NC_006570.2 624348 624536 R ORF ftt0605c; hypothetical protein complement(624348..624536) Francisella tularensis subsp. tularensis SCHU S4 3191160 YP_169628.1 CDS ispG NC_006570.2 625340 626557 D catalyzes the conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate into 4-hydroxy-3-methyl-2-en-1-yl diphosphate; involved in isoprenoid synthesis; 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase 625340..626557 Francisella tularensis subsp. tularensis SCHU S4 3190876 YP_169629.1 CDS FTT_0608 NC_006570.2 626572 627150 D Similar to Q9KHC2 Hypothetical 27.0 kDa protein from secondary endosymbiont of Glycaspis brimblecombei (244 aa). FASTA: opt: 467 Z-score: 605.3 E(): 8e-26 Smith-Waterman score: 467; 44.751identity in 181 aa overlap ORF ftt0608; hypothetical protein 626572..627150 Francisella tularensis subsp. tularensis SCHU S4 3191157 YP_169630.1 CDS FTT_0609 NC_006570.2 627301 629094 D Similar to Q83F75 Peptidase, M24 family protein from Coxiella burnetii (597 aa). FASTA: opt: 1665 Z-score: 1963.9 E(): 1.7e-101 Smith-Waterman score: 1665; 45.286 identity in 594 aa overlap ORF ftt0609; peptidase M24 family protein 627301..629094 Francisella tularensis subsp. tularensis SCHU S4 3191155 YP_169631.1 CDS FTT_0610 NC_006570.2 629192 630256 D Similar to Q8DFB3 DNA/RNA endonuclease G from Vibrio vulnificus (247 aa). FASTA: opt: 682 Z-score: 838.7 E(): 8e-39 Smith-Waterman score: 682; 46.311 identity in 244 aa overlap ORF ftt0610; DNA/RNA endonuclease family protein 629192..630256 Francisella tularensis subsp. tularensis SCHU S4 3191154 YP_169632.1 CDS FTT_0611c NC_006570.2 630247 631110 R Similar to Q9L6I3 Extended-spectrum beta-lactamase BES-1 from Serratia marcescens (292 aa).FASTA: opt: 621 Z-score: 723.1 E(): 2.2e-32 Smith-Waterman score: 621; 34.397 identity in 282 aa overlap ORF ftt0611c; beta-lactamase complement(630247..631110) Francisella tularensis subsp. tularensis SCHU S4 3191153 YP_169633.1 CDS FTT_0612 NC_006570.2 631546 632358 D ORF ftt0612; hypothetical protein 631546..632358 Francisella tularensis subsp. tularensis SCHU S4 3191152 YP_169634.1 CDS FTT_0613c NC_006570.2 632679 633098 R This ORF is longer. ORF ftt0613c; hypothetical protein complement(632679..633098) Francisella tularensis subsp. tularensis SCHU S4 3191151 YP_169635.1 CDS FTT_0614c NC_006570.2 633344 634834 R Similar to Q820B4 Apolipoprotein N-acyltransferase from Coxiella burnetii (485 aa). FASTA: opt: 984 Z-score: 1082.0 E(): 2.2e-52 Smith-Waterman score: 984; 35.903 identity in 493 aa overlap ORF ftt0614c; apolipoprotein N-acyltransferase complement(633344..634834) Francisella tularensis subsp. tularensis SCHU S4 3191149 YP_169636.1 CDS FTT_0615c NC_006570.2 634834 635694 R Similar to Q8EHP0 Magnesium and cobalt efflux protein CorC from Shewanella oneidensis (291 aa). FASTA: opt: 676 Z-score: 772.4 E(): 3.9e-35 Smith-Waterman score: 676; 39.847 identity in 261 aa overlap ORF ftt0615c; metal ion transporter protein complement(634834..635694) Francisella tularensis subsp. tularensis SCHU S4 3191148 YP_169637.1 CDS FTT_0616c NC_006570.2 635666 636172 R Similar to Q8EHN9 Hypothetical UPF0054 protein SO117 from Shewanella oneidensis (153 aa). FATSA: opt: 433 Z-score: 547.4 E(): 1.3e-22 Smith-Waterman score: 433; 44.025identity in 159 aa overlap ORF ftt0616c; hypothetical protein complement(635666..636172) Francisella tularensis subsp. tularensis SCHU S4 3191147 YP_169638.1 CDS phoH NC_006570.2 636147 637130 R Simliar to Q87VY0 PhoH-like protein from Pseudomonas syringae (340 aa). FASTA: opt: 1132 Z-score: 1306.4 E(): 7.1e-65 Smith-Waterman score: 1132; 52.923 identity in 325 aa overlap; phoH-like protein complement(636147..637130) Francisella tularensis subsp. tularensis SCHU S4 3191788 YP_169639.1 CDS yleA NC_006570.2 637149 638477 R catalyzes the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine from N6-(dimethylallyl)adenosine (i(6)A); (dimethylallyl)adenosine tRNA methylthiotransferase complement(637149..638477) Francisella tularensis subsp. tularensis SCHU S4 3192397 YP_169640.1 CDS FTT_0619 NC_006570.2 638700 639374 D Similar to O67476 O-methyltransferase from Aquifex aeolicus (212 aa). FASTA: opt: 382 Z-score: 477.6 E(): 1e-18 Smith-Waterman score: 382; 33.495 identity in 206 aa overlap. ORF ftt0619; o-methyltransferase family protein 638700..639374 Francisella tularensis subsp. tularensis SCHU S4 3191146 YP_169641.1 CDS FTT_0620 NC_006570.2 639383 640126 D Similar to Q8PER3 Acid phosphatase from Xanthomonas axonopodis (310 aa). FASTA: opt: 238 Z-score: 293.2 E(): 1.9e-08 Smith-Waterman score: 238; 24.583 identity in 240 aa overlap. ORF ftt0620; HAD superfamily protein 639383..640126 Francisella tularensis subsp. tularensis SCHU S4 3191144 YP_169642.1 CDS tdk NC_006570.2 640142 640735 D catalyzes the formation of thymidine 5'-phosphate from thymidine; thymidine kinase 640142..640735 Francisella tularensis subsp. tularensis SCHU S4 3190754 YP_169643.1 CDS FTT_0622c NC_006570.2 640778 641242 R ORF ftt0622c; hypothetical protein complement(640778..641242) Francisella tularensis subsp. tularensis SCHU S4 3191145 YP_169644.1 CDS tig NC_006570.2 641397 642713 D Tig; RopA; peptidyl-prolyl cis/trans isomerase; promotes folding of newly synthesized proteins; binds ribosomal 50S subunit; forms a homodimer; trigger factor 641397..642713 Francisella tularensis subsp. tularensis SCHU S4 3190785 YP_169645.1 CDS clpP NC_006570.2 642739 643344 D hydrolyzes proteins to small peptides; with the ATPase subunits ClpA or ClpX, ClpP degrades specific substrates; ATP-dependent Clp protease proteolytic subunit 642739..643344 Francisella tularensis subsp. tularensis SCHU S4 3192361 YP_169646.1 CDS clpX NC_006570.2 643368 644621 D binds and unfolds substrates as part of the ClpXP protease; ATP-dependent protease ATP-binding subunit ClpX 643368..644621 Francisella tularensis subsp. tularensis SCHU S4 3192507 YP_169647.1 CDS lon NC_006570.2 644646 646970 D Similar to Q8Z8V0 Lon protease from Salmonella typhi (784 aa). FASTA: opt: 2734 Z-score: 2797.3 E(): 6.4e-148 Smith-Waterman score: 2734; 54.450 identity in 764 aa overlap.; DNA-binding, ATP-dependent protease La 644646..646970 Francisella tularensis subsp. tularensis SCHU S4 3190802 YP_169648.1 CDS hupB NC_006570.2 647057 647329 D Similar to Q93TM3 Histone-like protein HU form B from Pseudomonas putida (strain KT2440) (90 aa). FASTA: opt: 380 Z-score: 519.0 E(): 5.2e-21 Smith-Waterman score: 380; 68.539 identity in 89 aa overlap.; histone-like protein HU form B 647057..647329 Francisella tularensis subsp. tularensis SCHU S4 3191898 YP_169649.1 CDS FTT_0628 NC_006570.2 647441 648871 D Similar to PPID_BUCAP (Q8K987) Peptidyl-prolyl cis-trans isomerase from Buchnera aphidicola (subsp. Schizaphis graminum) (621 aa). FASTA: opt: 298 Z-score: 309.1E(): 2.5e-09 Smith-Waterman score: 298; 23.362 identity in 351 aa overlap. 200 aa shorter (in C-term) than most of the homologs. ORF ftt0628; hypothetical protein 647441..648871 Francisella tularensis subsp. tularensis SCHU S4 3191142 YP_169650.1 CDS miaA NC_006570.2 648872 649798 D IPP transferase; isopentenyltransferase; involved in tRNA modification; in Escherichia coli this enzyme catalyzes the addition of a delta2-isopentenyl group from dimethylallyl diphosphate to the N6-nitrogen of adenosine adjacent to the anticodon of tRNA species that read codons starting with uracil; further tRNA modifications may occur; mutations in miaA result in defects in translation efficiency and fidelity; tRNA delta(2)-isopentenylpyrophosphate transferase 648872..649798 Francisella tularensis subsp. tularensis SCHU S4 3190845 YP_169651.1 CDS hfq NC_006570.2 649909 650238 D Similar to HFQ_PASMU (Q9CMC6) Hfq protein from Pasteurella multocida (95 aa). FASTA: opt: 384 Z-score: 504.4 E(): 3.3e-20 Smith-Waterman score: 384; 68.182 identity in 88 aa overlap. Plays a role in degradation of RNA transcripts.; host factor I for bacteriophage Q beta replication 649909..650238 Francisella tularensis subsp. tularensis SCHU S4 3192428 YP_169652.1 CDS hflX NC_006570.2 650290 651597 D Similar to Q87VJ5 GTP-binding protein HflX from Pseudomonas syringae (pv. tomato) (433 aa). FASTA: opt: 1331 Z-score: 1520.3 E(): 8.6e-77 Smith-Waterman score: 1331; 49.302 identity in 430 aa overlap. Together with HflC-HflK involved in stability of phage lambda cII repressor; protease GTP-binding subunit 650290..651597 Francisella tularensis subsp. tularensis SCHU S4 3192427 YP_169653.1 CDS FTT_0632c NC_006570.2 651608 652798 R Similar to O53772 oxidoreductase (Monooxygenase) from Mycobacterium tuberculosis (388 aa). FASTA: opt: 832 Z-score: 997.9 E(): 1.1e-47 Smith-Waterman score: 832; 35.550 identity in 391 aa overlap. ORF ftt0632c; hypothetical protein complement(651608..652798) Francisella tularensis subsp. tularensis SCHU S4 3191141 YP_169654.1 CDS hflK NC_006570.2 652970 654037 D Similar to Q8FAK7 HflK protein from Escherichia coli (419 aa). FASTA: opt: 843 Z-score: 932.9 E(): 4.5e-44 Smith-Waterman score: 843; 40.0 identity in 330 aa overlap. With HflC, part of modulator for protease specific for FtsH phage lambda cII repressor.; hypothetical protein 652970..654037 Francisella tularensis subsp. tularensis SCHU S4 3192426 YP_169655.1 CDS hflC NC_006570.2 654039 654965 D Similar to Q8EJ66 HflC protein from Shewanella oneidensis (297 aa). FASTA: opt: 799 Z-score: 895.3 E(): 5.6e-42 Smith-Waterman score: 799; 42.466 identity in 292 aa overlap. With HflK, part of modulator for protease specific for FtsH phage lambda cII repressor.; hypothetical protein 654039..654965 Francisella tularensis subsp. tularensis SCHU S4 3192425 YP_169656.1 CDS wzb NC_006570.2 654966 655451 D Similar to O35016 YFKJ protein from Bacillus subtilis (156 aa). FASTA: opt: 391 Z-score: 494.5 E(): 1.2e-19 Smith-Waterman score: 391; 38.608 identity in 158 aa overlap.; low molecular weight (LMW) phosphotyrosine protein phosphatase 654966..655451 Francisella tularensis subsp. tularensis SCHU S4 3192102 YP_169657.1 CDS engB NC_006570.2 655448 656041 D binds guanine nucleotides; in Escherichia coli depletion results in defective cell division and filamentation; in Bacillus subtilis this gene is essential; ribosome biogenesis GTP-binding protein YsxC 655448..656041 Francisella tularensis subsp. tularensis SCHU S4 3191566 YP_169658.1 CDS FTT_0638 NC_006570.2 657478 657828 D fragment. Weak similarity to leuA LE12_METJA (Q58595) 2-isopropylmalate synthase 2 from Methanococcus jannaschii (518 aa). FASTA: opt: 119 Z-score: 151.8 E(): 1.5 Smith-Waterman score: 119; 26.316 identity in 114 aa overlap (6-115:356-467). Next downstream ORF also has similarity to leuA. ORF ftt0638; hypothetical protein 657478..657828 Francisella tularensis subsp. tularensis SCHU S4 3191139 YP_169659.1 CDS FTT_0639 NC_006570.2 657870 657980 D fragment. Weak similarity to leuA LE12_METJA (Q58595) 2-isopropylmalate synthase 2 from Methanococcus jannaschii (518 aa). FASTA: opt: 86 Z-score: 142.0 E(): 5.1 Smith-Waterman score: 86; 50.000 identity in 32 aa overlap (1-32:479-510). Next upstream ORF also have similarity to leuA. ORF ftt0639; hypothetical protein 657870..657980 Francisella tularensis subsp. tularensis SCHU S4 3191138 YP_169660.1 CDS ilvD NC_006570.2 658004 659659 D catalyzes the dehydration of 2,3-dihydroxy-3-methylbutanoate to 3-methyl-2-oxobutanoate in valine and isoleucine biosynthesis; dihydroxy-acid dehydratase 658004..659659 Francisella tularensis subsp. tularensis SCHU S4 3191790 YP_169661.1 CDS ilvH NC_006570.2 661522 661839 D Similar to Q8A610 Acetohydroxyacid synthase small subunit (187 aa). FASTA: opt: 159 Z-score: 221.3 E(): 0.0002 Smith-Waterman score: 159; 33.333 identity in 75 aa overlap.; acetolactate synthase small subunit 661522..661839 Francisella tularensis subsp. tularensis SCHU S4 3191561 YP_169662.1 CDS mfd NC_006570.2 663034 666459 R Similar to Q9CM06 Mfd from Pasteurella multocida (1145 aa). FASTA: opt: 3312 Z-score: 3697.7 E(): 4.5e-198 Smith-Waterman score: 3312; 44.794 identity in 1143 aa overlap.; transcription-repair coupling factor complement(663034..666459) Francisella tularensis subsp. tularensis SCHU S4 3192296 YP_169663.1 CDS FTT_0645c NC_006570.2 666504 667190 R Similar to YCCA_ECOLI (P06967) Hypothetical protein yccA from Escherichia coli (219 aa). FASTA: opt: 588 Z-score: 603.2 E(): 1.1e-25 Smith-Waterman score: 588; 48.416 identity in 221 aa overlap. ORF ftt0645c; hypothetical protein complement(666504..667190) Francisella tularensis subsp. tularensis SCHU S4 3191066 YP_169664.1 CDS FTT_0646c NC_006570.2 667306 668565 R ORF ftt0646c; hypothetical protein complement(667306..668565) Francisella tularensis subsp. tularensis SCHU S4 3191065 YP_169665.1 CDS FTT_0647c NC_006570.2 668565 668996 R Similar to Q82WE7 Hypothetical protein from Nitrosomonas europaea (164 aa). FASTA: opt: 383 Z-score: 490.4 E(): 2e-19 Smith-Waterman score: 383; 41.606 identity in 137 aa overlap. ORF ftt0647c; hypothetical protein complement(668565..668996) Francisella tularensis subsp. tularensis SCHU S4 3191064 YP_169666.1 CDS nth NC_006570.2 668993 669631 R Similar to Q9HYB4 Endonuclease III from Pseudomonas aeruginosa (212 aa). FASTA: opt: 916 Z-score: 1153.9 E(): 2.2e-56 Smith-Waterman score: 916; 67.308identity in 208 aa overlap.; endonuclease III complement(668993..669631) Francisella tularensis subsp. tularensis SCHU S4 3191739 YP_169667.1 CDS rnfB NC_006570.2 669624 670253 R Similar to Q83B23 4Fe-4S binding domain protein from Coxiella burnetii (213 aa). FASTA: opt: 552 Z-score: 680.7 E(): 5e-30 Smith-Waterman score: 552; 42.788 identity in 208 aa overlap.; (Fe-S)-binding protein complement(669624..670253) Francisella tularensis subsp. tularensis SCHU S4 3191608 YP_169668.1 CDS grxB NC_006570.2 670255 670899 R cofactor involved in the reduction of disulfides; glutaredoxin complement(670255..670899) Francisella tularensis subsp. tularensis SCHU S4 3192277 YP_169669.1 CDS FTT_0651 NC_006570.2 670961 672529 D Similar to Q9L9P6 IraB from Legionella pneumophila (501 aa). FASTA: opt: 1486 Z-score: 1640.1 E(): 1.8e-83 Smith-Waterman score: 1486; 46.708 identity in 486 aa overlap. ORF ftt0651; proton-dependent oligopeptide transport (POT) family protein 670961..672529 Francisella tularensis subsp. tularensis SCHU S4 3191063 YP_169670.1 CDS ftnA NC_006570.2 672532 673029 R Similar to Q899N4 Ferritin from Clostridium tetani (169 aa). FASTA: opt: 527 Z-score: 679.5 E(): 5.9e-30 Smith-Waterman score: 527; 45.783identity in 166 aa overlap.; ferritin complement(672532..673029) Francisella tularensis subsp. tularensis SCHU S4 3191830 YP_169671.1 CDS lipA NC_006570.2 673236 674219 D catalyzes the radical-mediated insertion of two sulfur atoms into an acyl carrier protein (ACP) bound to an octanoyl group to produce a lipoyl group; lipoyl synthase 673236..674219 Francisella tularensis subsp. tularensis SCHU S4 3190780 YP_169672.1 CDS elbB NC_006570.2 674249 674908 D Similar to Q87IN9 Sigma cross-reacting protein 27A from Vibrio parahaemolyticus (216 aa). FASTA: opt: 683 Z-score: 873.4 E(): 9.3e-41 Smith-Waterman score: 683; 49.074 identity in 216 aa overlap.; isoprenoid biosynthesis protein with amidotransferase-like domain 674249..674908 Francisella tularensis subsp. tularensis SCHU S4 3191558 YP_169673.1 CDS FTT_0655 NC_006570.2 674924 675670 D Similar to YO32_SHEON (Q8EEF0) Hypothetical UPF0082 protein SO2432 from Shewanella oneidensis (248 aa). FASTA: opt: 1090 Z-score: 1308.8 E(): 5.2e-65 Smith-Waterman score: 1090; 64.516 identity in 248 aa overlap. ORF ftt0655; hypothetical protein 674924..675670 Francisella tularensis subsp. tularensis SCHU S4 3191062 YP_169674.1 CDS ruvC NC_006570.2 675746 676282 D endonuclease; resolves Holliday structures; forms a complex of RuvABC; the junction binding protein RuvA forms a hexameric ring along with the RuvB helicase and catalyzes branch migration; RuvC then interacts with RuvAB to resolve the Holliday junction by nicking DNA strands of like polarity; Holliday junction resolvase 675746..676282 Francisella tularensis subsp. tularensis SCHU S4 3192532 YP_169675.1 CDS ruvA NC_006570.2 677567 678220 D plays an essential role in ATP-dependent branch migration of the Holliday junction; Holliday junction DNA helicase RuvA 677567..678220 Francisella tularensis subsp. tularensis SCHU S4 3191358 YP_169676.1 CDS FTT_0659 NC_006570.2 678246 679658 D Similar to Q87TH5 Conserved hypothetical protein from Vibrio parahaemolyticus (514 aa). FASTA: opt: 1129 Z-score: 1083.4 E(): 1.9e-52 Smith-Waterman score: 1132; 42.000 identity in 450 aa overlap ORF ftt0659; DNA recombination protein RmuC family protein 678246..679658 Francisella tularensis subsp. tularensis SCHU S4 3191059 YP_169677.1 CDS FTT_0660 NC_006570.2 679707 680294 D ORF ftt0660; hypothetical protein 679707..680294 Francisella tularensis subsp. tularensis SCHU S4 3191060 YP_169678.1 CDS FTT_0661c NC_006570.2 680316 680612 R Similar to Q82LF3 Hypothetical protein from Streptomyces avermitilis (109 aa). FASTA: opt: 260 Z-score: 364.1 E(): 2.2e-12 Smith-Waterman score: 260; 47.778 identity in 90 aa overlap. The homologs are of different sizes. ORF ftt0661c; hypothetical protein complement(680316..680612) Francisella tularensis subsp. tularensis SCHU S4 3191057 YP_169680.1 CDS FTT_0663 NC_006570.2 681271 682083 D ORF ftt0663; hypothetical protein 681271..682083 Francisella tularensis subsp. tularensis SCHU S4 3191056 YP_169681.1 CDS hdc NC_006570.2 682070 683206 R catalyzes the formation of histamine from L-histidine; histidine decarboxylase complement(682070..683206) Francisella tularensis subsp. tularensis SCHU S4 3192413 YP_169682.1 CDS FTT_0665c NC_006570.2 683314 683919 R Similar to Q88IX8 Class II aldolase/adducin domain protein from Pseudomonas putida (strain KT2440) (251 aa). FASTA: opt: 472 Z-score: 599.3 E(): 1.7e-25 Smith-Waterman score: 472; 38.579identity in 197 aa overlap. ORF ftt0665c; aldolase complement(683314..683919) Francisella tularensis subsp. tularensis SCHU S4 3191054 YP_169683.1 CDS FTT_0666c NC_006570.2 684010 684591 R Similar to Q896H4 DNA-3-methyladenine glycosylase from Clostridium tetani (203 aa). FASTA: opt: 451 Z-score: 589.0 E(): 6.4e-25 Smith-Waterman score: 451; 42.784 identity in 194 aa overlap. ORF ftt0666c; methylpurine-DNA glycosylase family protein complement(684010..684591) Francisella tularensis subsp. tularensis SCHU S4 3191052 YP_169684.1 CDS FTT_0667 NC_006570.2 684719 685222 D ORF ftt0667; hypothetical protein 684719..685222 Francisella tularensis subsp. tularensis SCHU S4 3191053 YP_169685.1 CDS FTT_0668 NC_006570.2 685222 685854 D Similar to Q8A546 membrane transporter involved in nicotinamide mononucleotide transport from Bacteroides thetaiotaomicron (188 aa). FASTA: opt: 333 Z-score: 403.8 E(): 1.3e-14 Smith-Waterman score: 333; 29.016identity in 193 aa overlap. ORF ftt0668; nicotinamide mononucleotide transport (NMT) family protein 685222..685854 Francisella tularensis subsp. tularensis SCHU S4 3191048 YP_169686.1 CDS FTT_0669 NC_006570.2 685952 687127 D Similar to Q8GRF6 MagA protein from Magnetospirillum magnetotacticum (Aquaspirillum magnetotacticum) (432 aa). FASTA: opt: 769 Z-score: 826.0 E(): 4.1e-38 Smith-Waterman score: 769; 31.635identity in 373 aa overlap. ORF ftt0669; sodium/hydrogen exchanger family protein 685952..687127 Francisella tularensis subsp. tularensis SCHU S4 3191049 YP_169687.1 CDS FTT_0670c NC_006570.2 687460 687924 R ORF ftt0670c; hypothetical protein complement(687460..687924) Francisella tularensis subsp. tularensis SCHU S4 3191047 YP_169688.1 CDS FTT_0671 NC_006570.2 688237 689517 D Similar to Q83DX6 Major facilitator family transporter from Coxiella burnetii (421 aa). FASTA: opt: 689 Z-score: 731.9 E(): 7.1e-33 Smith-Waterman score: 689; 29.197identity in 411 aa overlap. ORF ftt0671; major facilitator transporter 688237..689517 Francisella tularensis subsp. tularensis SCHU S4 3191045 YP_169689.1 CDS FTT_0673c NC_006570.2 690789 691796 R Similar to Q8DF84 Predicted deacylase from Vibrio vulnificus (352 aa). FASTA: opt: 789 Z-score: 951.5 E(): 4.2e-45 Smith-Waterman score: 789; 34.328 identity in 335 aa overlap. ORF ftt0673c; hypothetical protein complement(690789..691796) Francisella tularensis subsp. tularensis SCHU S4 3191042 YP_169690.1 CDS prsA NC_006570.2 692007 692975 D Similar to KPRS_RALSO (Q8Y2E1) Ribose-phosphate pyrophosphokinase from Ralstonia solanacearum (Pseudomonas solanacearum) (316 aa). FASTA: opt: 1269 Z-score: 1520.3 E(): 8.6e-77 Smith-Waterman score: 1269; 58.413 identity in 315 aa overlap.; ribose-phosphate pyrophosphokinase 692007..692975 Francisella tularensis subsp. tularensis SCHU S4 3191230 YP_169691.1 CDS rplY NC_006570.2 693075 693365 D the Ctc family of proteins consists of two types, one that contains the N-terminal ribosomal protein L25 domain only which in Escherichia coli binds the 5S rRNA while a subset of proteins contain a C-terminal extension that is involved in the stress response; 50S ribosomal protein L25 693075..693365 Francisella tularensis subsp. tularensis SCHU S4 3191997 YP_169692.1 CDS FTT_0676 NC_006570.2 693461 694720 D Similar to Q83E77 Hypothetical protein from Coxiella burnetii (417 aa). FASTA: opt: 1049 Z-score: 1211.5 E(): 1.4e-59 Smith-Waterman score: 1049; 40.049 identity in 407 aa overlap. ORF ftt0676; hypothetical protein 693461..694720 Francisella tularensis subsp. tularensis SCHU S4 3191043 YP_169693.1 CDS FTT_0677c NC_006570.2 694925 695701 R Similar to Q8Z984 methyltransferase from Salmonella typhi (256 aa). FASTA: opt: 707 Z-score: 818.1 E(): 1.1e-37 Smith-Waterman score: 707; 42.570 identity in 249 aa overlap. ORF ftt0677c; hypothetical protein complement(694925..695701) Francisella tularensis subsp. tularensis SCHU S4 3191041 YP_169694.1 CDS FTT_0678c NC_006570.2 696310 696585 R ORF ftt0678c; hypothetical protein complement(696310..696585) Francisella tularensis subsp. tularensis SCHU S4 3191039 YP_169695.1 CDS FTT_0679c NC_006570.2 696600 697691 R translation-associated GTPase; the crystal structure of the Haemophilus influenzae YchF protein showed similarity to the yeast structure (PDB: 1NI3); fluorescence spectroscopy revealed nucleic acid binding; the yeast protein YBR025c interacts with the translation elongation factor eEF1; GTP-dependent nucleic acid-binding protein EngD complement(696600..697691) Francisella tularensis subsp. tularensis SCHU S4 3191040 YP_169696.1 CDS pth NC_006570.2 697710 698285 R Enables the recycling of peptidyl-tRNAs produced at termination of translation; peptidyl-tRNA hydrolase complement(697710..698285) Francisella tularensis subsp. tularensis SCHU S4 3191676 YP_169697.1 CDS blaA NC_006570.2 698278 699162 R Similar to BLAC_YEREN (Q01166) Beta-lactamase precursor from Yersinia enterocolitica (294 aa). FASTA: opt: 670 Z-score: 829.7 E(): 2.5e-38 Smith-Waterman score: 670; 36.268 identity in 284 aa overlap.; beta-lactamase class A complement(698278..699162) Francisella tularensis subsp. tularensis SCHU S4 3190964 YP_169698.1 CDS FTT_0682c NC_006570.2 699171 700016 R ORF ftt0682c; hypothetical protein complement(699171..700016) Francisella tularensis subsp. tularensis SCHU S4 3191037 YP_169699.1 CDS pilD NC_006570.2 700056 700874 R Similar to Q888U2 Type IV pilus prepilin peptidase PilD from Pseudomonas syringae (pv. tomato) (290 aa). FASTA: opt: 784 Z-score: 898.0 E(): 4e-42 Smith-Waterman score: 784; 44.531identity in 256 aa overlap. Cleaves the leader peptide of the fiber building block proteins and methylates the resulting N-terminal residue.; Type IV pili leader peptidase and methylase complement(700056..700874) Francisella tularensis subsp. tularensis SCHU S4 3191481 YP_169700.1 CDS sthA NC_006570.2 700878 702293 R catalyzes the conversion of NADPH to NADH; soluble pyridine nucleotide transhydrogenase complement(700878..702293) Francisella tularensis subsp. tularensis SCHU S4 3190728 YP_169701.1 CDS FTT_0685c NC_006570.2 702547 703530 R Similar to KCH_ECOLI (P31069) potassium channel protein (417 aa). FASTA: opt: 381 Z-score: 423.3 E(): 1.1e-15 Smith-Waterman score: 381; 31.203identity in 266 aa overlap. ORF ftt0685c; potassium channel protein complement(702547..703530) Francisella tularensis subsp. tularensis SCHU S4 3191036 YP_169702.1 CDS FTT_0686c NC_006570.2 703610 704914 R Similar to Q83E30 Proton/peptide symporter family protein from Coxiella burnetii (492 aa). FASTA: opt: 609 Z-score: 644.8 E(): 5e-28 Smith-Waterman score: 609; 26.545 identity in 437 aa overlap. The start is approx 55 aa later than the homologs. ORF ftt0686c; proton-dependent oligopeptide transport (POT) family protein complement(703610..704914) Francisella tularensis subsp. tularensis SCHU S4 3191033 YP_169703.1 CDS hslU NC_006570.2 705161 706528 R heat shock protein involved in degradation of misfolded proteins; ATP-dependent protease ATP-binding subunit HslU complement(705161..706528) Francisella tularensis subsp. tularensis SCHU S4 3192080 YP_169704.1 CDS hslV NC_006570.2 706536 707087 R heat shock protein involved in degradation of misfolded proteins; ATP-dependent protease peptidase subunit complement(706536..707087) Francisella tularensis subsp. tularensis SCHU S4 3191560 YP_169705.1 CDS FTT_0689 NC_006570.2 707279 708028 D ORF ftt0689; hypothetical protein 707279..708028 Francisella tularensis subsp. tularensis SCHU S4 3191031 YP_169706.1 CDS lplA NC_006570.2 708031 708933 R Similar to Q9KS71 Lipoate-protein ligase A from Vibrio cholerae (338 aa). FASTA: opt: 699 Z-score: 831.1 E(): 2.1e-38 Smith-Waterman score: 699; 38.545identity in 275 aa overlap.; lipoate-protein ligase A complement(708031..708933) Francisella tularensis subsp. tularensis SCHU S4 3192472 YP_169707.1 CDS tyrS NC_006570.2 709046 710236 D catalyzes the formation of tyrosyl-tRNA(Tyr) from tyrosine and tRNA(Tyr); tyrosyl-tRNA synthetase 709046..710236 Francisella tularensis subsp. tularensis SCHU S4 3191006 YP_169708.1 CDS isftu1 NC_006570.2 710737 711521 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(710737..711195,711195..711521)) Francisella tularensis subsp. tularensis SCHU S4 3190809 YP_169709.1 CDS mutM NC_006570.2 711592 712416 R Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases; formamidopyrimidine-DNA glycosylase complement(711592..712416) Francisella tularensis subsp. tularensis SCHU S4 3192054 YP_169710.1 CDS FTT_0694 NC_006570.2 712510 714609 D Similar to AAP18158 2-acyl-glycerophospho-ethanolamine acyltransferase,acyl-acyl-carrier protein synthetase from Shigella flexneri (719 aa). FASTA: opt: 735 Z-score: 862.5 E(): 3.8e-40 Smith-Waterman score: 826; 33.661identity in 508 aa overlap. n. b. No match between aa 1 and 196. Several domains missing compared to AAP18158 ORF ftt0694; hypothetical protein 712510..714609 Francisella tularensis subsp. tularensis SCHU S4 3191030 YP_169711.1 CDS mraW NC_006570.2 714615 715532 D Similar to AAP95225 S-adenosyl-methyltransferase MraW from Haemophilus ducreyi (313 aa). FASTA: opt: 918 Z-score: 1107.1 E(): 8.9e-54 Smith-Waterman score: 918; 49.677identity in 310 aa overlap.; S-adenosyl-methyltransferase MraW 714615..715532 Francisella tularensis subsp. tularensis SCHU S4 3191607 YP_169712.1 CDS FTT_0696 NC_006570.2 715529 715879 D Weak similarity to cell division protein ftsL. ORF ftt0696; hypothetical protein 715529..715879 Francisella tularensis subsp. tularensis SCHU S4 3191028 YP_169713.1 CDS ftsI NC_006570.2 715872 717566 D Similar to Q83F33 Penicillin-binding protein 3 from Coxiella burnetii (548 aa). FASTA: opt: 1361 Z-score: 1597.9 E(): 4.1e-81 Smith-Waterman score: 1361; 40.325 identity in 553 aa overlap.; peptidoglycan synthetase 715872..717566 Francisella tularensis subsp. tularensis SCHU S4 3191383 YP_169714.1 CDS rpsO NC_006570.2 717672 717938 D primary rRNA binding protein; helps nucleate assembly of 30S; binds directly to the 16S rRNA and an intersubunit bridge to the 23S rRNA; autoregulates translation through interactions with the mRNA leader sequence; 30S ribosomal protein S15 717672..717938 Francisella tularensis subsp. tularensis SCHU S4 3191158 YP_169715.1 CDS pnp NC_006570.2 717995 720076 D Similar to Q83D87 Polyribonucleotide nucleotidyltransferase from Coxiella burnetii (696 aa). FASTA: opt: 2437 Z-score: 2800.2 E(): 4.4e-148 Smith-Waterman score: 2437; 54.401identity in 693 aa overlap.; polynucleotide phosphorylase 717995..720076 Francisella tularensis subsp. tularensis SCHU S4 3191115 YP_169716.1 CDS FTT_0700 NC_006570.2 720082 720432 D essential respiratory protein A; may be involved in the transfer of iron-sulfur clusters; essential for growth using oxygen or alternate electron acceptors; iron-sulfur cluster insertion protein ErpA 720082..720432 Francisella tularensis subsp. tularensis SCHU S4 3191027 YP_169717.1 CDS kdsA NC_006570.2 720442 721290 D catalyzes the formation of 2-dehydro-3-deoxy-D-octonate 8-phosphate from phosphoenolpyruvate and D-arabinose 5-phosphate in LPS biosynthesis; 2-dehydro-3-deoxyphosphooctonate aldolase 720442..721290 Francisella tularensis subsp. tularensis SCHU S4 3192149 YP_169718.1 CDS udk NC_006570.2 721301 721966 D functions in pyrimidine salvage; pyrimidine ribonucleoside kinase; phosphorylates nucleosides or dinucleosides to make UMP or CMP using ATP or GTP as the donor; uridine kinase 721301..721966 Francisella tularensis subsp. tularensis SCHU S4 3192488 YP_169719.1 CDS rpoZ NC_006570.2 721959 722177 D Similar to RPOZ_PSEPK (Q88C82) Probable DNA-directed RNA polymerase omega chain from Pseudomonas putida (strain KT2440) (87 aa). FASTA: opt: 194 Z-score: 293.6 E(): 1.8e-08 Smith-Waterman score: 194; 52.857 identity in 70 aa overlap.; DNA-directed RNA polymerase subunit omega 721959..722177 Francisella tularensis subsp. tularensis SCHU S4 3191977 YP_169720.1 CDS FTT_0704 NC_006570.2 722442 723014 D ORF ftt0704; hypothetical protein 722442..723014 Francisella tularensis subsp. tularensis SCHU S4 3191026 YP_169721.1 CDS rumA NC_006570.2 723103 724452 D in Escherichia coli this enzyme catalyzes the SAM-dependent methylation of U1939 in the 23S ribomal RNA; binds an iron-sulfur cluster [4Fe-4S]; 23S rRNA 5-methyluridine methyltransferase 723103..724452 Francisella tularensis subsp. tularensis SCHU S4 3191516 YP_169722.1 CDS FTT_0707 NC_006570.2 725383 726177 D Similar to Q9CK00 Hypothetical protein PM1838 from Pasteurella multocida (234 aa). FASTA: opt: 456 Z-score: 507.2 E(): 2.3e-20 Smith-Waterman score: 456; 34.483identity in 232 aa overlap. ORF ftt0707; nicotinamide mononucleotide transport (NMT) family protein 725383..726177 Francisella tularensis subsp. tularensis SCHU S4 3191025 YP_169723.1 CDS FTT_0708 NC_006570.2 726265 727518 D Similar to Q83BD8 Major facilitator family transporter from Coxiella burnetii (411 aa). FASTA: opt: 868 Z-score: 941.6 E(): 1.5e-44 Smith-Waterman score: 868; 37.912identity in 364 aa overlap. ORF ftt0708; major facilitator transporter 726265..727518 Francisella tularensis subsp. tularensis SCHU S4 3191024 YP_169724.1 CDS eno NC_006570.2 727627 728997 D enolase; catalyzes the formation of phosphoenolpyruvate from 2-phospho-D-glycerate in glycolysis; phosphopyruvate hydratase 727627..728997 Francisella tularensis subsp. tularensis SCHU S4 3191859 YP_169725.1 CDS ftsB NC_006570.2 729005 729295 D Similar to FTSB_VIBPA (Q87LQ1) Cell division protein ftsB homolog from Vibrio parahaemolyticus (93 aa). FASTA: opt: 198 Z-score: 273.8 E(): 2.3e-07 Smith-Waterman score: 198; 37.209 identity in 86 aa overlap.; cell division protein 729005..729295 Francisella tularensis subsp. tularensis SCHU S4 3191079 YP_169726.1 CDS ispD NC_006570.2 729285 729974 D Similar to ISPD_PASMU (P57953) 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (238 aa). FASTA: opt: 506 Z-score: 656.8 E(): 1.1e-28 Smith-Waterman score: 506; 39.381 identity in 226 aa overlap.; 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 729285..729974 Francisella tularensis subsp. tularensis SCHU S4 3190877 YP_169727.1 CDS sdaC1 NC_006570.2 729979 731241 R Similar to SDAC_HAEIN (P44615) Serine transporter from Haemophilus influenzae (412 aa). FASTA: opt: 785 Z-score: 854.1 E(): 1.1e-39 Smith-Waterman score: 785; 33.099 identity in 426 aa overlap.; serine transporter complement(729979..731241) Francisella tularensis subsp. tularensis SCHU S4 3192377 YP_169728.1 CDS tdh NC_006570.2 731243 732298 R converts threonine and NAD to 1,2-amino-3-oxobutanoate and NADH; functions in threonine catabolism; L-threonine 3-dehydrogenase complement(731243..732298) Francisella tularensis subsp. tularensis SCHU S4 3190755 YP_169729.1 CDS kbl NC_006570.2 732321 733517 R catalyzes the formation of 2-amino-3-oxobutanoate from acetyl-CoA and glycine; 2-amino-3-ketobutyrate CoA ligase complement(732321..733517) Francisella tularensis subsp. tularensis SCHU S4 3192112 YP_169730.1 CDS FTT_0715 NC_006570.2 734050 736332 D Similar to Q9ZIX2 Chitinase c from Pseudoalteromonas sp. S9 (869 aa). FASTA: opt: 331 Z-score: 370.4 E(): 9.6e-13 Smith-Waterman score: 583; 24.111 identity in 900 aa overlap. The homologs are of varying sizes. ORF ftt0715; chitinase 734050..736332 Francisella tularensis subsp. tularensis SCHU S4 3191020 YP_169731.1 CDS upp NC_006570.2 736511 737155 D Catalyzes the formation of uracil and 5-phospho-alpha-D-ribosy 1-diphosphate from UMP and diphosphate; uracil phosphoribosyltransferase 736511..737155 Francisella tularensis subsp. tularensis SCHU S4 3191905 YP_169732.1 CDS FTT_0718 NC_006570.2 738163 738507 D ORF ftt0718; hypothetical protein 738163..738507 Francisella tularensis subsp. tularensis SCHU S4 3191021 YP_169733.1 CDS FTT_0719 NC_006570.2 738564 739787 D Aa 139-320 identical to AAP97846 Hypothetical protein (Fragment) from Francisella tularensis ssp. tularensis strain SchuS4 (184 aa). Similar to Q881T0 Proline/betaine transporter from Pseudomonas syringae (pv. tomato) (438 aa). FASTA: opt: 561 Z-score: 633.8 E(): 2.1e-27 Smith-Waterman score: 561; 25.616 identity in 406 aa overlap. ORF ftt0719; major facilitator transporter 738564..739787 Francisella tularensis subsp. tularensis SCHU S4 3191018 YP_169734.1 CDS dgt NC_006570.2 739771 741096 R Similar to DGT1_VIBPA (Q87R73) Deoxyguanosinetriphosphate triphosphohydrolase-like protein from Vibrio parahaemolyticus (440 aa). FASTA: opt: 587 Z-score: 683.8 E(): 3.4e-30 Smith-Waterman score: 661; 33.781identity in 447 aa overlap.; deoxyguanosinetriphosphate triphosphohydrolase complement(739771..741096) Francisella tularensis subsp. tularensis SCHU S4 3191520 YP_169735.1 CDS katG NC_006570.2 741200 743425 R Similar to CATA_YERPE (Q9X6B0) Peroxidase/catalase (EC 1.11.1.6) from Yersinia pestis (737 aa). FASTA: opt: 3041 Z-score: 3508.0 E(): 1.7e-187 Smith-Waterman score: 3041; 59.189 identity in 740 aa overlap.; peroxidase/catalase complement(741200..743425) Francisella tularensis subsp. tularensis SCHU S4 3192098 YP_169736.1 CDS isftu1 NC_006570.2 744289 745073 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(744289..744747,744747..745073)) Francisella tularensis subsp. tularensis SCHU S4 3191796 YP_169737.1 CDS FTT_0723c NC_006570.2 745380 746168 R Similar to Q88H18 Oxidoreductase, short chain dehydrogenase/reductase family Pseudomonas putida (strain KT2440) (248 aa). FASTA: opt: 792 Z-score: 881.9 E(): 3.1e-41 Smith-Waterman score: 792; 47.984 identity in 248 aa overlap. ORF ftt0723c; short-chain dehydrogenase complement(745380..746168) Francisella tularensis subsp. tularensis SCHU S4 3191019 YP_169738.1 CDS glpT NC_006570.2 747571 748887 R Similar to Q81V41 Glycerol-3-phosphate transporter from Bacillus anthracis (strain Ames) (449 aa). FASTA: opt: 1382 Z-score: 1521.3 E(): 7.6e-77 Smith-Waterman score: 1382; 48.268identity in 433 aa overlap.; glycerol-3-phosphate transporter complement(747571..748887) Francisella tularensis subsp. tularensis SCHU S4 3190969 YP_169739.1 CDS FTT_0726c NC_006570.2 748994 750028 R Similar to Q89FW5 Blr6584 protein from Bradyrhizobium japonicum (342 aa). FASTA: opt: 636 Z-score: 743.1 E(): 1.7e-33 Smith-Waterman score: 743; 36.336identity in 333 aa overlap. ORF ftt0726c; glycerophosphoryl diester phosphodiesterase family protein complement(748994..750028) Francisella tularensis subsp. tularensis SCHU S4 3191017 YP_169741.1 CDS ybhF NC_006570.2 751119 752051 D Similar to Q89II5 ABC transporter ATP-binding protein (310 aa). FASTA: opt: 1060 Z-score: 1223.8 E(): 2.8e-60 Smith-Waterman score: 1060; 54.605 identity in 304 aa overlap.; ABC transporter ATP-binding protein 751119..752051 Francisella tularensis subsp. tularensis SCHU S4 3192154 YP_169742.1 CDS ybhR NC_006570.2 752048 753166 D Similar to Q8Y316 Probable transmembrane protein from Ralstonia solanacearum (Pseudomonas solanacearum) (381 aa). FASTA: opt: 1138 Z-score: 1260.3 E(): 2.6e-62 Smith-Waterman score: 1138; 45.722 identity in 374 aa overlap.; ABC transporter permease 752048..753166 Francisella tularensis subsp. tularensis SCHU S4 3192155 YP_169743.1 CDS isftu2 NC_006570.2 753264 754007 D ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 transposase from Francisella tularensis (247 aa).; transposase 753264..754007 Francisella tularensis subsp. tularensis SCHU S4 3190910 YP_169745.1 CDS FTT_0732 NC_006570.2 754790 755137 D ORF ftt0732; hypothetical protein 754790..755137 Francisella tularensis subsp. tularensis SCHU S4 3192185 YP_169746.1 CDS FTT_0733 NC_006570.2 755166 755636 D Similar to Q9A7J3 Glutathione peroxidase from Caulobacter crescentus (160 aa). FASTA: opt: 605 Z-score: 801.1 E(): 9.9e-37 Smith-Waterman score: 605; 55.696 identity in 158 aa overlap. ORF ftt0733; glutathione peroxidase 755166..755636 Francisella tularensis subsp. tularensis SCHU S4 3192183 YP_169747.1 CDS FTT_0736 NC_006570.2 757924 758571 D Similar to Q8REM4 Hypothetical protein FN1075 (215 aa). FASTA: opt: 221 Z-score: 288.5 E(): 3.5e-08 Smith-Waterman score: 221; 25.463identity in 216 aa overlap. ORF ftt0736; hypothetical protein 757924..758571 Francisella tularensis subsp. tularensis SCHU S4 3192180 YP_169748.1 CDS FTT_0737c NC_006570.2 758582 758710 R ORF ftt0737c; hypothetical protein complement(758582..758710) Francisella tularensis subsp. tularensis SCHU S4 3192178 YP_169749.1 CDS yjeF NC_006570.2 758878 760422 D Similar to Q82Y67 Possible sugar kinase from Nitrosomonas europaea (505 aa). FASTA: Z-score: 922.3 E(): 1.8e-43 Smith-Waterman score: 856; 35.529 identity in 501 aa overlap.; carbohydrate kinase family protein 758878..760422 Francisella tularensis subsp. tularensis SCHU S4 3192390 YP_169750.1 CDS hslR NC_006570.2 760406 760774 R Similar to Q88AZ6 Heat shock protein 15 from Pseudomonas syringae (pv. tomato) (135 aa). FASTA: Z-score: 549.3 E(): 1.1e-22 Smith-Waterman score: 447; 58.824 identity in 119 aa overlap.; heat shock protein 15 complement(760406..760774) Francisella tularensis subsp. tularensis SCHU S4 3190937 YP_169753.1 CDS FTT_0742 NC_006570.2 763903 765891 D ORF ftt0742; lipoprotein 763903..765891 Francisella tularensis subsp. tularensis SCHU S4 3192174 YP_169754.1 CDS FTT_0743 NC_006570.2 766572 767387 D Similar to Q87Q96 Conserved hypothetical protein from Vibrio parahaemolyticus (274 aa). FASTA: opt: 950 Z-score: 1132.9 E(): 3.3e-55 Smith-Waterman score: 950; 49.259 identity in 270 aa overlap. ORF ftt0743; hypothetical protein 766572..767387 Francisella tularensis subsp. tularensis SCHU S4 3192172 YP_169758.1 CDS FTT_0747c NC_006570.2 769162 769947 R Similar to Q88NQ3 Membrane fusion protein, (289 aa). FASTA: opt: 721 Z-score: 853.4 E(): 1.2e-39 Smith-Waterman score: 721; 41.434identity in 251 aa overlap. Similar to ydhJ and yhcQ in E. coli. ORF ftt0747c; hypothetical protein complement(769162..769947) Francisella tularensis subsp. tularensis SCHU S4 3191854 YP_169759.1 CDS FTT_0748 NC_006570.2 770327 771046 D Similar to Q93PU6 regulatory protein TtgV from Pseudomonas putida (259 aa). FASTA: opt: 485 Z-score: 588.0 E(): 7.4e-25 Smith-Waterman score: 485; 33.473 identity in 239 aa overlap. ORF ftt0748; hypothetical protein 770327..771046 Francisella tularensis subsp. tularensis SCHU S4 3191851 YP_169760.1 CDS FTT_0749c NC_006570.2 771106 772092 R Aa 8-183 identical to AAP97845 Hypothetical protein (Fragment) from Francisella tularensis ssp. tularensis strain SchuS4 (176 aa). Similar to Q8CL71 Hypothetical from Yersinia pestis (311 aa). FASTA: opt: 842 Z-score: 992.9 E(): 2e-47 Smith-Waterman score: 842; 44.710 identity in 293 aa overlap ORF ftt0749c; hypothetical protein complement(771106..772092) Francisella tularensis subsp. tularensis SCHU S4 3191849 YP_169761.1 CDS FTT_0750 NC_006570.2 772298 773284 D Similar to Q83AM1 Radical SAM domain protein from Coxiella burnetii (342 aa). FASTA: opt: 1094 Z-score: 1304.2 E(): 9.4e-65 Smith-Waterman score: 1094; 48.930 identity in 327 aa overlap. ORF ftt0750; hypothetical protein 772298..773284 Francisella tularensis subsp. tularensis SCHU S4 3191848 YP_169762.1 CDS cspA NC_006570.2 773355 773558 R Similar to Q89UH2 Cold shock protein from Bradyrhizobium japonicum (70 aa). FASTA: opt: 297 Z-score: 443.7 E(): 8e-17 Smith-Waterman score: 297; 66.176 identity in 68 aa overlap.; cold shock protein complement(773355..773558) Francisella tularensis subsp. tularensis SCHU S4 3191900 YP_169764.1 CDS rpsU NC_006570.2 774330 774530 D a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA; 30S ribosomal protein S21 774330..774530 Francisella tularensis subsp. tularensis SCHU S4 3191263 YP_169765.1 CDS FTT_0754c NC_006570.2 774588 774767 R ORF ftt0754c; hypothetical protein complement(774588..774767) Francisella tularensis subsp. tularensis SCHU S4 3191844 YP_169766.1 CDS FTT_0755 NC_006570.2 775183 775677 D ORF ftt0755; hypothetical protein 775183..775677 Francisella tularensis subsp. tularensis SCHU S4 3191846 YP_169767.1 CDS FTT_0756 NC_006570.2 775768 776907 D Similar to Q83BX5 Cation-efflux family protein from Coxiella burnetii (378 aa). FASTA: opt: 773 Z-score: 908.9 E(): 9.8e-43 Smith-Waterman score: 774; 34.605 identity in 367 aa overlap. ORF ftt0756; cation-efflux family protein 775768..776907 Francisella tularensis subsp. tularensis SCHU S4 3191842 YP_169768.1 CDS galU NC_006570.2 776924 777787 D Similar to Q9K0G7 UTP--glucose-1-phosphate uridylyltransferase from Neisseria meningitidis (serogroup B) (289 aa). FASTA: opt: 1017 Z-score: 1291.1 E(): 5e-64 Smith-Waterman score: 1017; 57.439 identity in 289 aa overlap.; UTP-glucose-1-phosphate uridylyltransferase 776924..777787 Francisella tularensis subsp. tularensis SCHU S4 3191600 YP_169769.1 CDS FTT_0759 NC_006570.2 779260 780177 D Similar to YYAM_BACSU (P37511) Hypothetical transport protein yyaM from Bacillus subtilis (305 aa). FASTA: opt: 402 Z-score: 452.6 E(): 2.6e-17 Smith-Waterman score: 402; 25.614 identity in 285 aa overlap. ORF ftt0759; hypothetical protein 779260..780177 Francisella tularensis subsp. tularensis SCHU S4 3191841 YP_169770.1 CDS FTT_0760c NC_006570.2 780180 780386 R ORF ftt0760c; lipoprotein complement(780180..780386) Francisella tularensis subsp. tularensis SCHU S4 3191837 YP_169771.1 CDS FTT_0761c NC_006570.2 780471 780764 R ORF ftt0761c; hypothetical protein complement(780471..780764) Francisella tularensis subsp. tularensis SCHU S4 3191840 YP_169772.1 CDS recF NC_006570.2 780786 781835 R Similar to RECF_PROMI (P22839) DNA replication and repair protein from Proteus mirabilis (362 aa). FASTA: opt: 874 Z-score: 1010.7 E(): 2.1e-48 Smith-Waterman score: 874; 37.394 identity in 353 aa overlap.; DNA replication and repair protein RecF complement(780786..781835) Francisella tularensis subsp. tularensis SCHU S4 3191639 YP_169773.1 CDS yjjV NC_006570.2 781839 782585 R Similar to YJJV_ECOLI (P39408) deoxyribonuclease yjjV (259 aa). FASTA: opt: 503 Z-score: 595.3 E(): 2.9e-25 Smith-Waterman score: 503; 31.048 identity in 248 aa overlap.; TatD related DNAse family protein complement(781839..782585) Francisella tularensis subsp. tularensis SCHU S4 3192399 YP_169774.1 CDS glyS NC_006570.2 782778 784862 D Similar to SYGB_COXBU (P45651) Glycyl-tRNA synthetase beta chain from Coxiella burnetii (689 aa). FASTA: opt: 1810 Z-score: 2108.8 E(): 1.4e-109 Smith-Waterman score: 1810; 42.939 identity in 694 aa overlap.; glycyl-tRNA synthetase subunit beta 782778..784862 Francisella tularensis subsp. tularensis SCHU S4 3191775 YP_169775.1 CDS isftu1 NC_006570.2 785002 785786 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(785002..785328,785328..785786) Francisella tularensis subsp. tularensis SCHU S4 3190913 YP_169776.1 CDS deoD NC_006570.2 785955 786677 D Similar to Q9PQL0 Purine-nucleoside phosphorylase from Ureaplasma parvum (Ureaplasma urealyticum biotype 1) (239 aa). FASTA: opt: 697 Z-score: 837.2 E(): 9.7e-39 Smith-Waterman score: 697; 47.534 identity in 223 aa overlap.; purine nucleoside phosphorylase 785955..786677 Francisella tularensis subsp. tularensis SCHU S4 3191517 YP_169777.1 CDS FTT_0767c NC_006570.2 786674 787123 R ORF ftt0767c; hypothetical protein complement(786674..787123) Francisella tularensis subsp. tularensis SCHU S4 3191838 YP_169778.1 CDS FTT_0768c NC_006570.2 787127 788155 R ORF ftt0768c; hypothetical protein complement(787127..788155) Francisella tularensis subsp. tularensis SCHU S4 3191836 YP_169779.1 CDS secA NC_006570.2 788287 791007 D functions in protein export; can interact with acidic membrane phospholipids and the SecYEG protein complex; binds to preproteins; binds to ATP and undergoes a conformational change to promote membrane insertion of SecA/bound preprotein; ATP hydrolysis appears to drive release of the preprotein from SecA and deinsertion of SecA from the membrane; additional proteins SecD/F/YajC aid SecA recycling; exists in an equilibrium between monomers and dimers; may possibly form higher order oligomers; proteins in this cluster correspond SecA1; SecA2 is not essential and seems to play a role in secretion of a subset of proteins; preprotein translocase subunit SecA 788287..791007 Francisella tularensis subsp. tularensis SCHU S4 3192077 YP_169780.1 CDS rplU NC_006570.2 793076 793390 D Similar to AAP95195 50S ribosomal protein L21 from Haemophilus ducreyi (103 aa). FASTA: opt: 435 Z-score: 570.3 E(): 7.1e-24 Smith-Waterman score: 435; 66.019 identity in 103 aa overlap.; 50S ribosomal protein L21 793076..793390 Francisella tularensis subsp. tularensis SCHU S4 3192001 YP_169781.1 CDS rpmA NC_006570.2 793421 793675 D involved in the peptidyltransferase reaction during translation; 50S ribosomal protein L27 793421..793675 Francisella tularensis subsp. tularensis SCHU S4 3191996 YP_169782.1 CDS FTT_0774 NC_006570.2 794062 794439 D Similar to Q8DJY4 Tll1086 protein from Synechococcus elongatus (Thermosynechococcus elongatus) (126 aa). FASTA: opt: 516 Z-score: 715.9 E(): 5.5e-32 Smith-Waterman score: 516; 64.348 identity in 115 aa overlap. ORF ftt0774; hypothetical protein 794062..794439 Francisella tularensis subsp. tularensis SCHU S4 3191834 YP_169783.1 CDS rnd NC_006570.2 795675 796769 R Similar to Q8YGF6 Ribonuclease D from Brucella melitensis (385 aa). FASTA: opt: 538 Z-score: 584.5 E(): 1.2e-24 Smith-Waterman score: 538; 28.919 identity in 370 aa overlap.; ribonuclease D complement(795675..796769) Francisella tularensis subsp. tularensis SCHU S4 3191611 YP_169784.1 CDS uvrC NC_006570.2 796878 798716 D The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision; excinuclease ABC subunit C 796878..798716 Francisella tularensis subsp. tularensis SCHU S4 3191927 YP_169785.1 CDS FTT_0778 NC_006570.2 798783 799157 D ORF ftt0778; hypothetical protein 798783..799157 Francisella tularensis subsp. tularensis SCHU S4 3192437 YP_169786.1 CDS isftu1 NC_006570.2 799235 800019 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(799235..799561,799561..800019) Francisella tularensis subsp. tularensis SCHU S4 3190822 YP_169787.1 CDS FTT_0780c NC_006570.2 800295 800564 R ORF ftt0780c; hypothetical protein complement(800295..800564) Francisella tularensis subsp. tularensis SCHU S4 3192438 YP_169788.1 CDS FTT_0781c NC_006570.2 800554 801267 R Similar to Q82UL5 Hypothetical protein from Nitrosomonas europaea (239 aa). FASTA: opt: 736 Z-score: 920.8 E(): 2.1e-43 Smith-Waterman score: 736; 54.680 identity in 203 aa overlap. The family of possible lysine decarboxylase proteins shares a highly conserved motif PGGXGTXXE that is probably functionally important. This protein has that motif. ORF ftt0781c; hypothetical protein complement(800554..801267) Francisella tularensis subsp. tularensis SCHU S4 3191831 YP_169789.1 CDS fabI NC_006570.2 801553 802335 D Similar to Q87YS1 Enoyl-(acyl-carrier-protein) reductase from Pseudomonas syringae (pv. tomato) (264 aa). FASTA: opt: 1071 Z-score: 1313.1 E(): 3e-65 Smith-Waterman score: 1071; 62.595 identity in 262 aa overlap.; enoyl-ACP reductase 801553..802335 Francisella tularensis subsp. tularensis SCHU S4 3191864 YP_169790.1 CDS ars NC_006570.2 802482 803444 D Similar to Q9CPJ8 Arylsulfatase from Pasteurella multocida (327 aa). FASTA: opt: 544 Z-score: 669.4 E(): 2.2e-29 Smith-Waterman score: 544; 31.420 identity in 331 aa overlap.; arylsulfatase 802482..803444 Francisella tularensis subsp. tularensis SCHU S4 3190951 YP_169791.1 CDS FTT_0784 NC_006570.2 803456 803785 D ORF ftt0784; hypothetical protein 803456..803785 Francisella tularensis subsp. tularensis SCHU S4 3191824 YP_169792.1 CDS FTT_0785 NC_006570.2 803773 804594 D Similar to Q88K93 Conserved hypothetical protein (277 aa). FASTA: opt: 718 Z-score: 855.8 E(): 8.9e-40 Smith-Waterman score: 718; 42.308identity in 260 aa overlap. ORF ftt0785; hypothetical protein 803773..804594 Francisella tularensis subsp. tularensis SCHU S4 3191822 YP_169793.1 CDS FTT_0786 NC_006570.2 804591 805289 D ORF ftt0786; hypothetical protein 804591..805289 Francisella tularensis subsp. tularensis SCHU S4 3191821 YP_169794.1 CDS FTT_0787 NC_006570.2 805300 805890 D Similar to Q8EDN3 Conserved hypothetical protein from Shewanella oneidensis (197 aa). FASTA: opt: 435 Z-score: 540.9 E(): 3.1e-22 Smith-Waterman score: 435; 42.935 identity in 184 aa overlap. ORF ftt0787; DoxD-like family protein 805300..805890 Francisella tularensis subsp. tularensis SCHU S4 3191820 YP_169795.1 CDS kdsD NC_006570.2 805894 806877 R Similar to Q8KLW6 Hypothetical protein from Pseudomonas stutzeri (Pseudomonas perfectomarina) (324 aa). FASTA: opt: 1095 Z-score: 1307.1 E(): 6.5e-65 Smith-Waterman score: 1095; 54.489 identity in 323 aa overlap. Homologs are involved in sialic acid capsule biosynthesis in some pathogenic strains of Escherichia coli.; arabinose phosphate isomerase complement(805894..806877) Francisella tularensis subsp. tularensis SCHU S4 3192129 YP_169796.1 CDS rpe NC_006570.2 807002 807670 D Similar to Q8EK14 Ribulose-phosphate 3-epimerase (225 aa). FASTA: opt: 953 Z-score: 1164.4 E(): 5.8e-57 Smith-Waterman score: 953; 65.766identity in 222 aa overlap.; D-ribulose-phosphate 3-epimerase 807002..807670 Francisella tularensis subsp. tularensis SCHU S4 3192026 YP_169797.1 CDS FTT_0790 NC_006570.2 807651 809045 D Similar to Q9FDJ5 glycosyltransferase from Lactobacillus rhamnosus (466 aa). FASTA: opt: 733 Z-score: 876.0 E(): 6.7e-41 Smith-Waterman score: 733; 33.333 identity in 399 aa overlap. ORF ftt0790; sugar transferase 807651..809045 Francisella tularensis subsp. tularensis SCHU S4 3191819 YP_169798.1 CDS galE NC_006570.2 809038 810057 D UDP-glucose 4-epimerase 809038..810057 Francisella tularensis subsp. tularensis SCHU S4 3191584 YP_169799.1 CDS FTT_0792 NC_006570.2 810061 811290 D Similar to Q9HTC0 Glycosyltransferase WbpZ from Pseudomonas aeruginosa (381 aa). FASTA: opt: 621 Z-score: 721.7 E(): 2.6e-32 Smith-Waterman score: 1019; 38.725 identity in 408 aa overlap. ORF ftt0792; glycosyl transferase family protein 810061..811290 Francisella tularensis subsp. tularensis SCHU S4 3191845 YP_169800.1 CDS FTT_0793 NC_006570.2 811287 812969 D Similar to Q8DMA7 ATP-binding protein of ABC transporter from Synechococcus elongatus (Thermosynechococcus elongatus) (610 aa). FASTA: opt: 1084 Z-score: 1105.1 E(): 1.2e-53 Smith-Waterman score: 1084; 35.159 identity in 566 aa overlap. ORF ftt0793; ABC transporter ATP-binding protein/permease 811287..812969 Francisella tularensis subsp. tularensis SCHU S4 3191818 YP_169801.1 CDS FTT_0794 NC_006570.2 812983 814269 D Similar to AAP76675 Phosphoserine phosphatase from Helicobacter hepaticus (199 aa). FASTA: opt: 751 Z-score: 908.2 E(): 1.1e-42 Smith-Waterman score: 751; 55.385 identity in 195 aa overlap (18-212:4-198) AND to AAP76674 Hypothetical protein from Helicobacter hepaticus (213 aa). FASTA: opt: 823 Z-score: 994.3 E(): 1.7e-47 Smith-Waterman score: 823; 57.212 identity in 208 aa overlap (221-428:6-213). Seems to be two genes in some organisms. ORF ftt0794; hypothetical protein 812983..814269 Francisella tularensis subsp. tularensis SCHU S4 3191816 YP_169802.1 CDS FTT_0795 NC_006570.2 814208 814891 D Similar to AAP76671 Hypothetical protein cfa from Helicobacter hepaticus (210 aa). FASTA: opt: 627 Z-score: 745.4 E(): 1.3e-33 Smith-Waterman score: 627; 44.660 identity in 206 aa overlap. ORF ftt0795; hypothetical protein 814208..814891 Francisella tularensis subsp. tularensis SCHU S4 3191817 YP_169803.1 CDS FTT_0796 NC_006570.2 814895 815656 D ORF ftt0796; hypothetical protein 814895..815656 Francisella tularensis subsp. tularensis SCHU S4 3191828 YP_169804.1 CDS FTT_0797 NC_006570.2 815665 816624 D Similar to O85000 Galactosyl transferase from Streptococcus pneumoniae (323 aa). FASTA: opt: 577 Z-score: 682.7 E(): 3.9e-30 Smith-Waterman score: 577; 40.171 identity in 234 aa overlap. Same homolog as for next downstream ORF. ORF ftt0797; glycosyl transferase family protein 815665..816624 Francisella tularensis subsp. tularensis SCHU S4 3191827 YP_169805.1 CDS FTT_0798 NC_006570.2 816641 817648 D Similar to O85000 Galactosyl transferase from Streptococcus pneumoniae (323 aa). FASTA: opt: 499 Z-score: 609.1 E(): 4.9e-26 Smith-Waterman score: 499; 34.454 identity in 238 aa overlap. Same homolog as for next upstream ORF. ORF ftt0798; glycosyl transferase family protein 816641..817648 Francisella tularensis subsp. tularensis SCHU S4 3191812 YP_169806.1 CDS FTT_0799 NC_006570.2 817651 818664 D Similar to Q00481 Second mannosyl transferase from Salmonella enterica (336 aa). FASTA: opt: 651 Z-score: 775.2 E(): 2.7e-35 Smith-Waterman score: 651; 35.455 identity in 330 aa overlap. ORF ftt0799; glycosyl transferases group 1 family protein 817651..818664 Francisella tularensis subsp. tularensis SCHU S4 3191811 YP_169807.1 CDS FTT_0800 NC_006570.2 818683 819345 D Similar to Q8FV27 Hydrolase, haloacid dehalogenase-like family from Brucella suis (234 aa). FASTA: opt: 422 Z-score: 503.3 E(): 3.8e-20 Smith-Waterman score: 422; 33.486identity in 218 aa overlap. ORF ftt0800; haloacid dehalogenase 818683..819345 Francisella tularensis subsp. tularensis SCHU S4 3191810 YP_169808.1 CDS FTT_0801c NC_006570.2 819627 820733 R Similar to Q9YG89 310AA long hypothetical sugar kinase from Aeropyrum pernix (310 aa). FASTA: opt: 245 Z-score: 292.6 E(): 2.1e-08 Smith-Waterman score: 311; 25.795 identity in 283 aa overlap. ORF ftt0801c; carbohydrate/purine kinase pfkB family protein complement(819627..820733) Francisella tularensis subsp. tularensis SCHU S4 3191809 YP_169809.1 CDS cphB NC_006570.2 820936 821754 D Similar to CPHB_SYNY8 (P56946) Cyanophycinase from Synechocystis sp. (strain PCC 6308) (272 aa). FASTA: opt: 701 Z-score: 857.3 E(): 7.4e-40 Smith-Waterman score: 701; 41.667 identity in 264 aa overlap.; cyanophycinase 820936..821754 Francisella tularensis subsp. tularensis SCHU S4 3191887 YP_169810.1 CDS ans NC_006570.2 821970 822833 D Similar to Q9ABX3 Asparaginase family protein from Caulobacter crescentus (292 aa). FASTA: 146 opt: 465 Z-score: 567.7 E(): 1e-23 Smith-Waterman score: 465; 35.606 identity in 264 aa overlap.; asparaginase 821970..822833 Francisella tularensis subsp. tularensis SCHU S4 3190806 YP_169811.1 CDS FTT_0804 NC_006570.2 823505 824761 D Similar to O54543 (O54543) CITA from Legionella pneumophila (418 aa). FASTA: opt: 905 Z-score: 915.1 E(): 4.5e-43 Smith-Waterman score: 905; 33.254 identity in 418 aa overlap. ORF ftt0804; major facilitator transporter 823505..824761 Francisella tularensis subsp. tularensis SCHU S4 3191808 YP_169812.1 CDS capB NC_006570.2 824837 826054 D Similar to CAD71904 Capsule biosynthesis protein CapB from Rhodopirellula baltica (441 aa). FASTA: opt: 1155 Z-score: 1414.5 E(): 6.8e-71 Smith-Waterman score: 1156; 46.763 identity (50.914 ungapped) in 417 aa overlap.; capsule biosynthesis protein CapB 824837..826054 Francisella tularensis subsp. tularensis SCHU S4 3190971 YP_169813.1 CDS capC NC_006570.2 826079 826543 D Similar to Q8REZ4 CapC protein from Fusobacterium nucleatum (152 aa). FASTA: opt: 360 Z-score: 420.9 E(): 1.5e-15 Smith-Waterman score: 360; 35.616 identity in 146 aa overlap.; capsule biosynthesis protein CapC 826079..826543 Francisella tularensis subsp. tularensis SCHU S4 3192366 YP_169814.2 CDS FTT_0807 NC_006570.2 826545 827756 D Similar to CAD71902 Hypothetical protein from Rhodopirellula baltica (404 aa). FASTA: opt: 477 Z-score: 563.9 E(): 1.6e-23 Smith-Waterman score: 553; 31.633 identity in 392 aa overlap. ORF ftt0807; hypothetical protein 826545..827756 Francisella tularensis subsp. tularensis SCHU S4 3191829 YP_169815.1 CDS spoT NC_006570.2 827816 829939 D Similar to SPOT_ECOLI Guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase from Escherichia coli (702 aa). FASTA: opt: 1849 Z-score: 2107.7 E(): 1.7e-109 Smith-Waterman score: 1849; 41.210 identity in 711 aa overlap.; guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase 827816..829939 Francisella tularensis subsp. tularensis SCHU S4 3190723 YP_169816.1 CDS recR NC_006570.2 829947 830549 R involved in a recombinational process of DNA repair, independent of the recBC complex; recombination protein RecR complement(829947..830549) Francisella tularensis subsp. tularensis SCHU S4 3191634 YP_169817.1 CDS ybaB NC_006570.2 830549 830887 R Similar to YBAB_ECOLI Hypothetical UPF0133 protein ybaB from Escherichia coli (109 aa). FASTA: opt: 332 Z-score: 403.8 E(): 1.3e-14 Smith-Waterman score: 332; 49.074identity in 108 aa overlap.; hypothetical protein complement(830549..830887) Francisella tularensis subsp. tularensis SCHU S4 3192128 YP_169818.1 CDS murG NC_006570.2 830897 832012 R UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase; involved in cell wall formation; inner membrane-associated; last step of peptidoglycan synthesis; undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase complement(830897..832012) Francisella tularensis subsp. tularensis SCHU S4 3192028 YP_169819.1 CDS gcvH2 NC_006570.2 832116 832496 D Similar to GCS1_SULTO Probable glycine cleavage system H from Sulfolobus tokodaii (142 aa). FASTA: opt: 397 Z-score: 525.8 E(): 2.1e-21 Smith-Waterman score: 397; 43.089 identity in 123 aa overlap.; glycine cleavage system protein H 832116..832496 Francisella tularensis subsp. tularensis SCHU S4 3191479 YP_169820.1 CDS FTT_0813 NC_006570.2 832486 832782 D ORF ftt0813; hypothetical protein 832486..832782 Francisella tularensis subsp. tularensis SCHU S4 3190926 YP_169824.1 CDS thrS NC_006570.2 835100 837004 D catalyzes a two-step reaction, first charging a threonine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; catalyzes the formation of threonyl-tRNA(Thr) from threonine and tRNA(Thr); threonyl-tRNA synthetase 835100..837004 Francisella tularensis subsp. tularensis SCHU S4 3190763 YP_169825.1 CDS infC NC_006570.2 837080 837547 D Similar to IF3_ECOLI Translation initiation factor IF-3 from Escherichia coli (180 aa). FASTA: opt: 674 Z-score: 836.1 E(): 1.1e-38 Smith-Waterman score: 674; 64.103 identity in 156 aa overlap.; translation initiation factor IF-3 837080..837547 Francisella tularensis subsp. tularensis SCHU S4 3191791 YP_169826.1 CDS rpmI NC_006570.2 837590 837787 D Similar to RL35_ECOLI 50S ribosomal protein L35 from Escherichia coli (64 aa). FASTA: opt: 247 Z-score: 385.9 E(): 1.3e-13 Smith-Waterman score: 247; 62.500 identity in 64 aa overlap.; 50S ribosomal protein L35 837590..837787 Francisella tularensis subsp. tularensis SCHU S4 3191986 YP_169827.1 CDS rplT NC_006570.2 837823 838179 D binds directly to 23S ribosomal RNA prior to in vitro assembly of the 50S ribosomal subunit; 50S ribosomal protein L20 837823..838179 Francisella tularensis subsp. tularensis SCHU S4 3192002 YP_169828.1 CDS FTT_0821 NC_006570.2 838221 838925 D Similar to Q9KRB9 Hypothetical protein VC1723 from Vibrio cholerae (229 aa). FASTA: opt: 344 Z-score: 394.7 E(): 4.3e-14 Smith-Waterman score: 344; 29.075 identity in 227 aa overlap. ORF ftt0821; hypothetical protein 838221..838925 Francisella tularensis subsp. tularensis SCHU S4 3192335 YP_169829.1 CDS isftu1 NC_006570.2 839217 840001 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(839217..839543,839543..840001) Francisella tularensis subsp. tularensis SCHU S4 3191797 YP_169830.1 CDS FTT_0823 NC_006570.2 840225 840872 D Similar to Q9K8B7 Mutator MutT protein from Bacillus halodurans (207 aa). FASTA: opt: 413 Z-score: 505.4 E(): 2.9e-20 Smith-Waterman score: 413; 36.649identity in 191 aa overlap. ORF ftt0823; MutT protein 840225..840872 Francisella tularensis subsp. tularensis SCHU S4 3191835 YP_169831.1 CDS arsC1 NC_006570.2 840890 841243 D Similar to Q8CPW9 Arsenate reductase from Staphylococcus epidermidis (117 aa). FASTA: opt: 276 Z-score: 376.2 E(): 4.6e-13 Smith-Waterman score: 276; 32.759 identity in 116 aa overlap.; arsenate reductase 840890..841243 Francisella tularensis subsp. tularensis SCHU S4 3192546 YP_169832.1 CDS FTT_0825c NC_006570.2 841267 841602 R hypothetical lipoprotein, no significant homologs. ORF ftt0825c; hypothetical protein complement(841267..841602) Francisella tularensis subsp. tularensis SCHU S4 3191761 YP_169833.1 CDS FTT_0826c NC_006570.2 841779 842024 R ORF ftt0826c; lipoprotein complement(841779..842024) Francisella tularensis subsp. tularensis SCHU S4 3191760 YP_169834.1 CDS FTT_0827c NC_006570.2 842102 843415 R Similar to YIEG_ECOLI Hypothetical protein yieG from Escherichia coli (445 aa). FASTA: opt: 945 Z-score: 1037.7 E(): 6.6e-50 Smith-Waterman score: 945; 34.633 identity in 436 aa overlap. ORF ftt0827c; hypothetical protein complement(842102..843415) Francisella tularensis subsp. tularensis SCHU S4 3191758 YP_169835.1 CDS FTT_0829c NC_006570.2 845080 846795 R Similar to Q845W9 Aspartate:alanine antiporter from Pseudomonas dacunhae (561 aa). FASTA: opt: 1359 Z-score: 1502.2 bits: 287.8 E(): 8.8e-76 Smith-Waterman score: 1359; 38.351 identity in 558 aa overlap. ORF ftt0829c; aspartate:alanine antiporter complement(845080..846795) Francisella tularensis subsp. tularensis SCHU S4 3191754 YP_169836.1 CDS FTT_0831c NC_006570.2 847950 849203 R Weakly similar to Q8A8H3 Outer membrane protein,OmpA family from Bacteroides thetaiotaomicron (266 aa). FASTA: opt: 184 Z-score: 220.7 E(): 0.00021 Smith-Waterman score: 184; 29.299 identity in 157 aa overlap. ORF ftt0831c; OmpA family protein complement(847950..849203) Francisella tularensis subsp. tularensis SCHU S4 3191752 YP_169837.1 CDS fkpB NC_006570.2 849395 849859 D Similar to FKBX_ECOLI (P22563) FKBP-type 16 kDa peptidyl-prolyl cis-trans isomerase from Escherichia coli (148 aa). FASTA: opt: 357 Z-score: 475.0 E(): 1.4e-18 Smith-Waterman score: 357; 38.129 identity in 139 aa overlap.; FKBP-type peptidylprolyl isomerase 849395..849859 Francisella tularensis subsp. tularensis SCHU S4 3191946 YP_169838.1 CDS ispH NC_006570.2 849840 850796 D catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP); functions in the nonmevalonate isoprenoid biosynthesis pathway; 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 849840..850796 Francisella tularensis subsp. tularensis SCHU S4 3190808 YP_169839.1 CDS aroQ NC_006570.2 850798 851160 D Similar to CHMU_BACSU Chorismate mutase from Bacillus subtilis (127 aa). FASTA: opt: 360 Z-score: 492.8 E(): 1.5e-19 Smith-Waterman score: 360; 48.305identity in 118 aa overlap.; chorismate mutase 850798..851160 Francisella tularensis subsp. tularensis SCHU S4 3190933 YP_169840.1 CDS FTT_0835 NC_006570.2 851169 851990 D Similar to Q8EBE8 CDP-diacylglycerol--serine O-phosphatidyltransferase from Shewanella oneidensis (269 aa). FASTA: opt: 456 Z-score: 550.1 E(): 9.5e-23 Smith-Waterman score: 564; 37.209 identity in 258 aa overlap. ORF ftt0835; CDP-alcohol phosphatidyltransferase 851169..851990 Francisella tularensis subsp. tularensis SCHU S4 3191751 YP_169841.1 CDS FTT_0836c NC_006570.2 851995 852219 R ORF ftt0836c; hypothetical protein complement(851995..852219) Francisella tularensis subsp. tularensis SCHU S4 3191749 YP_169842.1 CDS tolQ NC_006570.2 852360 853079 D Similar to TOLQ_ECOLI TolQ protein from Escherichia coli (230 aa). FASTA: opt: 568 Z-score: 690.3 E(): 1.5e-30 Smith-Waterman score: 568; 38.991 identity in 218 aa overlap.; TolQ protein 852360..853079 Francisella tularensis subsp. tularensis SCHU S4 3191457 YP_169843.1 CDS tolR NC_006570.2 853089 853535 D Similar to TOLR_ECOLI TolR protein from Escherichia coli (142 aa). FASTA: opt: 303 Z-score: 379.2 bits: 76.1 E(): 3.2e-13 Smith-Waterman score: 303; 39.259identity in 135 aa overlap.; TolR protein 853089..853535 Francisella tularensis subsp. tularensis SCHU S4 3191469 YP_169844.1 CDS tolA NC_006570.2 853522 854433 D FTT0839 Contains similarity to Pfam domain PF06519 (TolA protein), Score=-66.2, E-value=1.4e-05; hypothetical protein 853522..854433 Francisella tularensis subsp. tularensis SCHU S4 3191750 YP_169845.1 CDS tolB NC_006570.2 854433 855752 D Similar to TOLB_ECOLI TolB protein precursor from Eschericia coli (430 aa). FASTA: opt: 692 Z-score: 785.2 E(): 7.6e-36 Smith-Waterman score: 717; 31.685identity in 445 aa overlap. TolB appears more highly conserved in other species: potential paralog; TolB protein precursor 854433..855752 Francisella tularensis subsp. tularensis SCHU S4 3190788 YP_169846.1 CDS FTT_0841 NC_006570.2 855756 856007 D ORF ftt0841; lipoprotein 855756..856007 Francisella tularensis subsp. tularensis SCHU S4 3191747 YP_169847.1 CDS FTT_0842 NC_006570.2 856026 856649 D Similar to PAL_ECOLI Peptidoglycan-associated lipoprotein from Escherichia coli (173 aa). FASTA: opt: 306 Z-score: 383.2 E(): 1.9e-13 Smith-Waterman score: 306; 33.333 identity in 162 aa overlap. ORF ftt0842; peptidoglycan-associated lipoprotein 856026..856649 Francisella tularensis subsp. tularensis SCHU S4 3191746 YP_169848.1 CDS FTT_0845 NC_006570.2 858877 859152 D Similar to Q9K315 Hypothetical protein VCA0343 OR VCA029 from Vibrio cholerae (83 aa). FASTA: opt: 250 Z-score: 356.2 E(): 6e-12 Smith-Waterman score: 250; 58.462 identity (58.4620ngapped) in 65 aa overlap. ORF ftt0845; hypothetical protein 858877..859152 Francisella tularensis subsp. tularensis SCHU S4 3191743 YP_169849.1 CDS FTT_0846 NC_006570.2 859133 860632 D Similar to Q9KS67 Deoxyribodipyrimidine photolyase, from Vibrio cholerae (504 aa). FASTA: opt: 1071 Z-score: 1263.0 E(): 1.8e-62 Smith-Waterman score: 1097; 37.817 identity in 513 aa overlap. ORF ftt0846; deoxyribodipyrimidine photolyase 859133..860632 Francisella tularensis subsp. tularensis SCHU S4 3191742 YP_169850.1 CDS FTT_0847 NC_006570.2 860686 862161 D Similar to Q8U6P2 Hypothetical protein Atu4765 (507 aa). FASTA: opt: 1800 Z-score: 2192.9 415.3 E(): 3e-114 Smith-Waterman score: 1800; 53.608identity in 485 aa overlap. ORF ftt0847; hypothetical protein 860686..862161 Francisella tularensis subsp. tularensis SCHU S4 3191738 YP_169851.1 CDS FTT_0848 NC_006570.2 862158 862295 D Similar to Q8P3G1 Hypothetical protein XCC4110 from Xanthomonas campestris (51 aa). FASTA: opt: 243 Z-score: 366.5 E(): 1.6e-12 Smith-Waterman score: 243; 63.636 identity in 44 aa overlap ORF ftt0848; hypothetical protein 862158..862295 Francisella tularensis subsp. tularensis SCHU S4 3191736 YP_169852.1 CDS FTT_0849 NC_006570.2 862421 863350 D Similar to Q81YH5 Bile acid transporter family protein from Bacillus anthracis (322 aa). FASTA: initn: 551 init1: 399 opt: 605 Z-score: 641.1 E(): 8.1e-28 Smith-Waterman score: 605; 32.432identity in 296 aa overlap. ORF ftt0849; bile acid symporter family protein 862421..863350 Francisella tularensis subsp. tularensis SCHU S4 3191737 YP_169853.1 CDS FTT_0850 NC_006570.2 863400 864023 D Similar to Q930D7 Hypothetical protein RA0261 Rhizobium meliloti (222 aa). FASTA: opt: 521 Z-score: 614.9 E(): 2.3e-26 Smith-Waterman score: 521; 42.424 identity in 198 aa overlap. ORF ftt0850; hypothetical protein 863400..864023 Francisella tularensis subsp. tularensis SCHU S4 3191735 YP_169854.1 CDS FTT_0851 NC_006570.2 864047 864805 D Similar to Q83C04 Membrane protein, from Coxiella burnetii (263 aa). FASTA: opt: 338 Z-score: 387.6 E(): 1.1e-13 Smith-Waterman score: 341; 28.625 identity in 269 aa overlap. ORF ftt0851; hypothetical protein 864047..864805 Francisella tularensis subsp. tularensis SCHU S4 3191740 YP_169855.1 CDS FTT_0853 NC_006570.2 866499 867737 D Similar to Q8PY17 Arsenite permease from Methanosarcina mazei (420 aa). FASTA: opt: 1294 Z-score: 1359.3 E(): 8e-68 Smith-Waterman score: 1294; 49.144 identity in 409 aa overlap. ORF ftt0853; arsenite permease family protein 866499..867737 Francisella tularensis subsp. tularensis SCHU S4 3191733 YP_169856.1 CDS FTT_0854c NC_006570.2 867716 868138 R Similar to Q8EM34 Hypothetical conserved protein from Oceanobacillus iheyensis (123 aa). FASTA: opt: 271 Z-score: 341.6 E(): 3.9e-11 Smith-Waterman score: 271; 37.190 identity in 121 aa overlap. ORF ftt0854c; hypothetical protein complement(867716..868138) Francisella tularensis subsp. tularensis SCHU S4 3191732 YP_169857.1 CDS FTT_0855c NC_006570.2 868158 868517 R Weakly similar to Q9KR35 Hypothetical protein VC1812 from Vibrio cholerae (139 aa). FASTA: opt: 184 Z-score: 256.0 E(): 2.3e-06 Smith-Waterman score: 184; 29.245 identity in 106 aa overlap. ORF ftt0855c; hypothetical protein complement(868158..868517) Francisella tularensis subsp. tularensis SCHU S4 3191731 YP_169858.1 CDS ubiA NC_006570.2 868531 869385 R UbiA prenyltransferase family catalyzes the transfer of a prenyl group to various acceptors with hydrophobic ring structures in the biosynthesis of respiratory quinones, hemes, chlorophylls, vitamin E, and shikonin; 4-hydroxybenzoate polyprenyltransferase complement(868531..869385) Francisella tularensis subsp. tularensis SCHU S4 3192470 YP_169859.1 CDS FTT_0857c NC_006570.2 869388 869894 R Weakly similar to similar to Q83F94 Chorismate--pyruvate lyase, from Coxiella burnetii (185 aa). FASTA: opt: 178 Z-score: 231.0 E(): 5.6e-05 Smith-Waterman score: 178; 27.215identity in 158 aa overlap ORF ftt0857c; hypothetical protein complement(869388..869894) Francisella tularensis subsp. tularensis SCHU S4 3191729 YP_169860.1 CDS rph NC_006570.2 869993 870700 D RNase PH; tRNA nucleotidyltransferase; forms hexamers in Bacillus subtilis; phosphoroltic 3'-5' exoribonuclease; involved in maturation of tRNA precursors and removes terminal nucleotides near CCA acceptor arms of mature tRNAs; ribonuclease PH 869993..870700 Francisella tularensis subsp. tularensis SCHU S4 3192025 YP_169861.1 CDS FTT_0859c NC_006570.2 870754 871077 R ORF ftt0859c; hypothetical protein complement(870754..871077) Francisella tularensis subsp. tularensis SCHU S4 3191728 YP_169862.1 CDS FTT_0860c NC_006570.2 871182 871433 R ORF ftt0860c; hypothetical protein complement(871182..871433) Francisella tularensis subsp. tularensis SCHU S4 3191726 YP_169863.1 CDS FTT_0861c NC_006570.2 871496 872089 R Similar to FMA2_BACNO (P17824) Fimbrial protein precursor (Pilin) from Bacteroides nodosus (Dichelobacter nodosus) (160 aa). FASTA: opt: 169 Z-score: 222.1 E(): 0.00018 Smith-Waterman score: 169; 34.646 identity in 127 aa overlap The pili fiber building block protein has a highly distinct primary structure; a short positively charged leader sequence, and a highly conserved and highly hydrophobic amino-terminal domain which forms the core of the pilus fiber; A variety of studies suggest that the invariant glycine (G) at -1 is required for prepilin cleavage and assembly and that the glutamate (E) at + 5 is essential for assembly and efficient N-terminal methylation but not cleavage. The region near the carboxy terminus is often hydrophilic and is stabilized by one or two disulfide bridges that may be involved in recognition of receptors. The genes with these characteristics are usually termed pilA, pilE, pilV, pilW, pilX and FimU. (Mattick John S.. 'Type IV Pili and twitching motility'. Annu. Rev. Microbiol. 2002. 56:289-314.) This protein has all the characteristics described above, but shows a weak global homology. ORF ftt0861c; Type IV pili fiber building block protein complement(871496..872089) Francisella tularensis subsp. tularensis SCHU S4 3191727 YP_169864.1 CDS htpX NC_006570.2 872194 873267 R metalloprotease; heat shock protein HtpX complement(872194..873267) Francisella tularensis subsp. tularensis SCHU S4 3191549 YP_169865.1 CDS FTT_0863c NC_006570.2 873271 873846 R Similar to Q83DZ3 LemA protein from Coxiella burnetii (192 aa) initn: 723 init1: 655 opt: 732 Z-score: 803.2 bits: 155.4 E(): 7.6e-37 Smith-Waterman score: 732; 60.417 identity in 192 aa overlap. ORF ftt0863c; LemA-like protein complement(873271..873846) Francisella tularensis subsp. tularensis SCHU S4 3191725 YP_169866.1 CDS FTT_0864c NC_006570.2 873894 874790 R Similar to Q87XZ5 Transcriptional regulator, LysR family, from Pseudomonas syringae (308 aa). FASTA: opt: 591 Z-score: 726.5 E(): 1.4e-32 Smith-Waterman score: 591; 34.948 identity in 289 aa overlap. ORF ftt0864c; LysR family transcriptional regulator complement(873894..874790) Francisella tularensis subsp. tularensis SCHU S4 3191724 YP_169867.1 CDS isftu2 NC_006570.2 876765 877508 D ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 transposase from Francisella tularensis (247 aa).; transposase 876765..877508 Francisella tularensis subsp. tularensis SCHU S4 3190911 YP_169868.1 CDS FTT_0868c NC_006570.2 879013 879378 R Similar to Q9RA93 ArsR from Sinorhizobium sp. As4 (115 aa). FASTA: opt: 185 Z-score: 259.0 bits: 53.3 E(): 1.6e-06 Smith-Waterman score: 185; 27.184 identity in 103 aa overlap. ORF ftt0868c; arsenical restistance operon repressor complement(879013..879378) Francisella tularensis subsp. tularensis SCHU S4 3191721 YP_169869.1 CDS FTT_0869 NC_006570.2 879521 880174 D ORF ftt0869; hypothetical protein 879521..880174 Francisella tularensis subsp. tularensis SCHU S4 3191720 YP_169870.1 CDS FTT_0870c NC_006570.2 880186 880317 R ORF ftt0870c; hypothetical protein complement(880186..880317) Francisella tularensis subsp. tularensis SCHU S4 3191718 YP_169871.1 CDS gpsA NC_006570.2 880494 881492 D catalyzes the NAD(P)H-dependent reduction of glycerol 3-phosphate to glycerone phosphate; NAD(P)H-dependent glycerol-3-phosphate dehydrogenase 880494..881492 Francisella tularensis subsp. tularensis SCHU S4 3192551 YP_169872.1 CDS FTT_0872c NC_006570.2 881493 882029 R ORF ftt0872c; hypothetical protein complement(881493..882029) Francisella tularensis subsp. tularensis SCHU S4 3191717 YP_169873.1 CDS radA NC_006570.2 882034 883404 R Sms; stabilizes the strand-invasion intermediate during the DNA repair; involved in recombination of donor DNA and plays an important role in DNA damage repair after exposure to mutagenic agents; DNA repair protein RadA complement(882034..883404) Francisella tularensis subsp. tularensis SCHU S4 3191647 YP_169874.1 CDS FTT_0874c NC_006570.2 883412 884182 R ORF ftt0874c; hypothetical protein complement(883412..884182) Francisella tularensis subsp. tularensis SCHU S4 3191715 YP_169875.1 CDS FTT_0875c NC_006570.2 884179 884778 R Similar to YOHD_ECOLI (P33366) Hypothetical protein yohD from Escherichia coli (192 aa). FASTA: opt: 311 Z-score: 379.7 bits: 77.1 E(): 2.9e-13 Smith-Waterman score: 311; 24.870 identity (25.806 ungapped) in 193 aa overlap. ORF ftt0875c; hypothetical protein complement(884179..884778) Francisella tularensis subsp. tularensis SCHU S4 3191716 YP_169876.1 CDS aroC NC_006570.2 884781 885839 R catalyzes the formation of chorismate from 5-O-(1-carboxyvinyl)-3-phosphoshikimate in aromatic amino acid biosynthesis; chorismate synthase complement(884781..885839) Francisella tularensis subsp. tularensis SCHU S4 3190943 YP_169877.1 CDS FTT_0877c NC_006570.2 886004 886633 R ORF ftt0877c; hypothetical protein complement(886004..886633) Francisella tularensis subsp. tularensis SCHU S4 3191714 YP_169878.1 CDS msrB NC_006570.2 886652 887164 R this stereospecific enzymes reduces the R isomer of methionine sulfoxide while MsrA reduces the S form; provides protection against oxidative stress; methionine sulfoxide reductase B complement(886652..887164) Francisella tularensis subsp. tularensis SCHU S4 3191245 YP_169879.1 CDS sodC NC_006570.2 887325 887882 D superoxide dismutase (Cu-Zn) precusor. Identical to SODC_FRATU from F. tularensis LVS.; superoxide dismuate 887325..887882 Francisella tularensis subsp. tularensis SCHU S4 3190715 YP_169880.1 CDS FTT_0881c NC_006570.2 888650 890044 R Similar to AAP09927 Arginine permease from Bacillus cereus (474 aa). FASTA: opt: 1041 Z-score: 1224.1 E(): 2.5e-60 Smith-Waterman score: 1041; 35.604 identity in 455 aa overlap. ORF ftt0881c; amino acid permease complement(888650..890044) Francisella tularensis subsp. tularensis SCHU S4 3191712 YP_169881.1 CDS aspC1 NC_006570.2 891809 892999 R catalyzes the formation of oxalozcetate and L-glutamate from L-aspartate and 2-oxoglutarate; aspartate aminotransferase complement(891809..892999) Francisella tularensis subsp. tularensis SCHU S4 3190956 YP_169882.1 CDS FTT_0885 NC_006570.2 893288 894067 D Similar to Q8D6R6 Predicted Co/Zn/Cd cation transporter from Vibrio vulnificus (297 aa). FASTA: opt: 344 Z-score: 414.1 E(): 3.3e-15 Smith-Waterman score: 344; 25.296 identity in 253 aa overlap. ORF ftt0885; cation transporter 893288..894067 Francisella tularensis subsp. tularensis SCHU S4 3191707 YP_169883.1 CDS FTT_0886 NC_006570.2 894173 895822 D Similar to P52118 DNA repair protein recN (Recombination protein (554 aa) fasta scores; opt: 1371 Z-score: 1221.7 E(): 3.4e-60 Smith-Waterman score: 1371; 39.674 identity in 552 aa overlap. ORF ftt0886; DNA repair protein RecN 894173..895822 Francisella tularensis subsp. tularensis SCHU S4 3191706 YP_169884.1 CDS FTT_0887c NC_006570.2 896394 896768 R ORF ftt0887c; hypothetical protein complement(896394..896768) Francisella tularensis subsp. tularensis SCHU S4 3191705 YP_169885.1 CDS FTT_0888c NC_006570.2 896761 897357 R Similar to Q59607 Pilin precursor from Neisseria gonorrhoeae (169 aa). FASTA: opt: 244 Z-score: 305.7 E(): 3.5e-09 Smith-Waterman score: 244; 30.435 identity in 138 aa overlap. The pili fiber building block protein has a highly distinct primary structure; a short positively charged leader sequence, and a highly conserved and highly hydrophobic amino-terminal domain which forms the core of the pilus fiber; A variety of studies suggest that the invariant glycine (G) at -1 is required for prepilin cleavage and assembly and that the glutamate (E) at + 5 is essential for assembly and efficient N-terminal methylation but not cleavage. The region near the carboxy terminus is often hydrophilic and is stabilized by one or two disulfide bridges that may be involved in recognition of receptors. The genes with these characteristics are usually termed pilA, pilE, pilV, pilW, pilX and FimU. (Mattick John S.. 'Type IV Pili and twitching motility'. Annu. Rev. Microbiol. 2002. 56:289-314.) This protein has all the characteristics described above. ORF ftt0888c; Type IV pili fiber building block protein complement(896761..897357) Francisella tularensis subsp. tularensis SCHU S4 3191703 YP_169886.1 CDS FTT_0889c NC_006570.2 898027 898467 R Similar to Q820M2 Fimbrial protein pilin:bacterial general secretion pathway protein H from Nitrosomonas europaea (136 aa). FASTA: opt: 216 Z-score: 280.1 E(): 1e-07 Smith-Waterman score: 218; 32.639 identity in 144 aa overlap. The pili fiber building block protein has a highly distinct primary structure; a short positively charged leader sequence, and a highly conserved and highly hydrophobic amino-terminal domain which forms the core of the pilus fiber; A variety of studies suggest that the invariant glycine (G) at -1 is required for prepilin cleavage and assembly and that the glutamate (E) at + 5 is essential for assembly and efficient N-terminal methylation but not cleavage. The region near the carboxy terminus is often hydrophilic and is stabilized by one or two disulfide bridges that may be involved in recognition of receptors. The genes with these characteristics are usually termed pilA, pilE, pilV, pilW, pilX and FimU. (Mattick John S.. 'Type IV Pili and twitching motility'. Annu. Rev. Microbiol. 2002. 56:289-314.) This protein has all the characteristics described above. ORF ftt0889c; Type IV pili fiber building block protein complement(898027..898467) Francisella tularensis subsp. tularensis SCHU S4 3191704 YP_169887.1 CDS FTT_0890c NC_006570.2 898598 899005 R Similar to Q8Y1X9 Type 4 fimbrial pilin signal peptide protein from Ralstonia solanacearum (168 aa). FASTA: opt: 278 Z-score: 327.6 E(): 2.1e-10 Smith-Waterman score: 292; 38.095identity in 147 aa overlap. The pili fiber building block protein has a highly distinct primary structure; a short positively charged leader sequence, and a highly conserved and highly hydrophobic amino-terminal domain which forms the core of the pilus fiber; A variety of studies suggest that the invariant glycine (G) at -1 is required for prepilin cleavage and assembly and that the glutamate (E) at + 5 is essential for assembly and efficient N-terminal methylation but not cleavage. The region near the carboxy terminus is often hydrophilic and is stabilized by one or two disulfide bridges that may be involved in recognition of receptors. The genes with these characteristics are usually termed pilA, pilE, pilV, pilW, pilX and FimU. (Mattick John S.. 'Type IV Pili and twitching motility'. Annu. Rev. Microbiol. 2002. 56:289-314.) This protein has all the characteristics described above. ORF ftt0890c; Type IV pili fiber building block protein complement(898598..899005) Francisella tularensis subsp. tularensis SCHU S4 3191700 YP_169888.1 CDS FTT_0891 NC_006570.2 899389 900108 D Similar to Q98NS1 Hypothetical protein mlr0012 from Rhizobium loti (244 aa). FASTA: opt: 327 Z-score: 404.4 bits: 82.2 E(): 1.2e-14 Smith-Waterman score: 327; 32.308 identity in 195 aa overlap. ORF ftt0891; hypothetical protein 899389..900108 Francisella tularensis subsp. tularensis SCHU S4 3191699 YP_169889.1 CDS folD NC_006570.2 900247 901095 D catalyzes the formation of 5,10-methenyltetrahydrofolate from 5,10-methylenetetrahydrofolate and subsequent formation of 10-formyltetrahydrofolate from 5,10-methenyltetrahydrofolate; bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methylene-tetrahydrofolate cyclohydrolase 900247..901095 Francisella tularensis subsp. tularensis SCHU S4 3191934 YP_169890.1 CDS purM NC_006570.2 901329 902372 D catalyzes the formation of 1-(5-phosphoribosyl)-5-aminoimidazole from 2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in purine biosynthesis; phosphoribosylaminoimidazole synthetase 901329..902372 Francisella tularensis subsp. tularensis SCHU S4 3191664 YP_169891.1 CDS purCD NC_006570.2 902372 904684 D Amino acids 1-327 (PurC) similar to AAR06292 SAICAR synthetase from Nicotiana tabacum (399 aa). BLAST: Expect = e-105 Identities = 187/322 (58), Positives = 236/322 (73), Gaps = 6/322 (1). Amino acids 328-770 (PurD) similar to ZP_00077753 Phosphoribosylamine-glycine ligase from Methanosarcina barkeri (433). BLAST: Expect = e-108 Identities = 204/436 (46), Positives = 285/436 (65), Gaps = 3/436 (0); fusion protein PurC/PurD 902372..904684 Francisella tularensis subsp. tularensis SCHU S4 3191239 YP_169892.1 CDS purN NC_006570.2 904688 905263 D Similar to Q8DBC3 Folate-dependent phosphoribosylglycinamide formyltransferase from Vibrio vulnificus (212 aa). FASTA: opt: 530 Z-score: 633.0 E(): 2.1e-27 Smith-Waterman score: 530; 45.263 identity in 190 aa overlap.; phosphoribosylglycinamide formyltransferase 904688..905263 Francisella tularensis subsp. tularensis SCHU S4 3191663 YP_169893.1 CDS purE NC_006570.2 905380 905871 D Similar to P72157 Phosphoribosylaminoimidazole carboxylase catalytic subunit from Psudomonas aeruginosa (163 aa). FASTA: opt: 719 Z-score: 894.1 E(): 6.6e-42 Smith-Waterman score: 719; 70.988 identity n 162 aa overlap; phosphoribosylaminoimidazole carboxylase catalyic subunit 905380..905871 Francisella tularensis subsp. tularensis SCHU S4 3191675 YP_169894.1 CDS purK NC_006570.2 905875 906972 D With PurE catalyzes the conversion of aminoimidazole ribonucleotide to carboxyaminoimidazole ribonucleotide in the de novo purine nucleotide biosynthetic pathway; phosphoribosylaminoimidazole carboxylase ATPase subunit 905875..906972 Francisella tularensis subsp. tularensis SCHU S4 3191666 YP_169895.1 CDS FTT_0898c NC_006570.2 906969 907322 R Similar to Q88N88 Conserved hypothetical protein TIGR002 from Pseudomonas putida (124 aa). FASTA: opt: 291 Z-score: 402.6 E(): 1.6e-14 Smith-Waterman score: 291; 38.938 identity in 113 aa overlap. ORF ftt0898c; hypothetical protein complement(906969..907322) Francisella tularensis subsp. tularensis SCHU S4 3191698 YP_169896.1 CDS prlC NC_006570.2 907389 909410 R Similar to P27298 Oligopeptidase A from Escherichia coli (680 aa). FASTA: opt: 1936 Z-score: 2040.0 E(): 8.9e-106 Smith-Waterman score: 1936; 43.722 identity in 677 aa overlap.; oligopeptidase A complement(907389..909410) Francisella tularensis subsp. tularensis SCHU S4 3191236 YP_169897.1 CDS FTT_0900 NC_006570.2 909502 909873 D ORF ftt0900; hypothetical protein 909502..909873 Francisella tularensis subsp. tularensis SCHU S4 3191697 YP_169898.1 CDS lpnA NC_006570.2 909954 910403 D Paralog to conserved hypothetical lipoprotein lpnB.; lipoprotein 909954..910403 Francisella tularensis subsp. tularensis SCHU S4 3191792 YP_169899.1 CDS FTT_0902 NC_006570.2 910562 911041 D paralog of FTT0903 ORF ftt0902; hypothetical protein 910562..911041 Francisella tularensis subsp. tularensis SCHU S4 3191696 YP_169900.1 CDS FTT_0903 NC_006570.2 911141 911644 D paralog of FTT0902 ORF ftt0903; hypothetical protein 911141..911644 Francisella tularensis subsp. tularensis SCHU S4 3191693 YP_169901.1 CDS lpnB NC_006570.2 911677 912144 D Similar to P18149 17 kDa major membrane protein precursor from Francisella tularensis ssp. holarctica strain LVS (149 aa). FASTA: opt: 274 Z-score: 359.1 E(): 4.1e-12 Smith-Waterman score: 274; 32.468 identity (33.5570ngapped) in 154 aa overlap. Paralog to conserved hypothetical lipoprotein lpnA.; lipoprotein 911677..912144 Francisella tularensis subsp. tularensis SCHU S4 3190834 YP_169902.1 CDS FTT_0905 NC_006570.2 912217 913659 D Similar to Q8KQ19 PilO from Pseudomonas aeruginosa (461 aa). FASTA: opt: 283 Z-score: 329.7 E(): 1.6e-10 Smith-Waterman score: 283; 25.284 identity in 352 aa overlap. PilO is required for glycosylation of Pseudomonas aeruginosa pilin. ORF ftt0905; Type IV pili glycosylation protein 912217..913659 Francisella tularensis subsp. tularensis SCHU S4 3191692 YP_169903.1 CDS topA NC_006570.2 913713 916007 R catalyzes the ATP-dependent breakage of single-stranded DNA followed by passage and rejoining; maintains net negative superhelicity; DNA topoisomerase I complement(913713..916007) Francisella tularensis subsp. tularensis SCHU S4 3190793 YP_169904.1 CDS parA NC_006570.2 916206 916847 D Similar to Q9F260 Partition protein from Actinobacillus actinomycetemcomitans (213 aa). FASTA: opt: 286 Z-score: 339.2 E(): 4.8e-11 mith-Waterman score: 286; 29.245 identity in 212 aa overlap; chmomosome partition protein A 916206..916847 Francisella tularensis subsp. tularensis SCHU S4 3191395 YP_169905.1 CDS parB NC_006570.2 916840 917754 D Similar to Q9CJA4 Chromosome partitioning protein from Lactococcus lactis (261 aa). FASTA: opt: 399 Z-score: 442.0 E(): 9e-17 Smith-Waterman score: 399; 33.333 identity in 264 aa overlap; chromosome partition protein B 916840..917754 Francisella tularensis subsp. tularensis SCHU S4 3191409 YP_169906.1 CDS FTT_0909 NC_006570.2 917768 918475 D Similar to Q88LT5 Glutamine amidotransferase, class I from Pseudomonas putida (244 aa). FASTA: opt: 589 Z-score: 771.5 bits: 150.1 E(): 4.4e-35 Smith-Waterman score: 589; 36.325identity in 234 aa overlap. ORF ftt0909; glutamine amidotransferase 917768..918475 Francisella tularensis subsp. tularensis SCHU S4 3191690 YP_169907.1 CDS FTT_0910 NC_006570.2 918478 919359 D Weakly similar to Q8GNM7 Lysophospholipase A from Legionella pneumophila (309 aa). FASTA: 86 opt: 302 Z-score: 378.8 bits: 78.1 E(): 3.3e-13 Smith-Waterman score: 360; 29.795 identity in 292 aa overlap. ORF ftt0910; hypothetical protein 918478..919359 Francisella tularensis subsp. tularensis SCHU S4 3191688 YP_169908.1 CDS FTT_0911 NC_006570.2 919356 919946 D Similar with Q87NK1 Hypothetical protein from Vibrio parahaemolyticus (195 aa) fasta scores; opt: 523 Z-score: 649.0 E(): 2.7e-28 Smith-Waterman score: 523; 42.857 identity in 189 aa overlap ORF ftt0911; hypothetical protein 919356..919946 Francisella tularensis subsp. tularensis SCHU S4 3191687 YP_169909.1 CDS rrmJ NC_006570.2 920006 920626 R Similar to Q8EHM3 Ribosomal RNA large subunit methyltransferase J from Shewanella oneidensis (209 aa). FASTA: opt: 730 Z-score: 844.0 E(): 3.7e-39 Smith-Waterman score: 730; 53.202identity in 203 aa overlap; ribosomal large subunit methyltransferase J complement(920006..920626) Francisella tularensis subsp. tularensis SCHU S4 3191489 YP_169910.1 CDS FTT_0913 NC_006570.2 920784 921251 D ORF ftt0913; hypothetical protein 920784..921251 Francisella tularensis subsp. tularensis SCHU S4 3191683 YP_169911.1 CDS lspA NC_006570.2 921246 921731 R lipoprotein signal peptidase; integral membrane protein that removes signal peptides from prolipoproteins during lipoprotein biosynthesis; lipoprotein signal peptidase complement(921246..921731) Francisella tularensis subsp. tularensis SCHU S4 3190811 YP_169912.1 CDS ileS NC_006570.2 921718 924525 R IleRS; catalyzes the formation of isoleucyl-tRNA(Ile) from isoleucine and tRNA(Ile); since isoleucine and other amino acids such as valine are similar, there are additional editing function in this enzyme; one is involved in hydrolysis of activated valine-AMP and the other is involved in deacylation of mischarged Val-tRNA(Ile); there are two active sites, one for aminoacylation and one for editing; class-I aminoacyl-tRNA synthetase family type 1 subfamily; some organisms carry two different copies of this enzyme; isoleucyl-tRNA synthetase complement(921718..924525) Francisella tularensis subsp. tularensis SCHU S4 3192543 YP_169913.1 CDS ribF NC_006570.2 924618 925538 R Similar to Q9CKF8 RibF from Pasteurella multocida (311 aa). FASTA: opt: 845 Z-score: 994.4 E(): 1.5e-47 Smith-Waterman score: 845; 44.481 identity in 308 aa overlap; riboflavin biosynthesis protein RibF complement(924618..925538) Francisella tularensis subsp. tularensis SCHU S4 3191623 YP_169914.1 CDS maeA NC_006570.2 925689 927503 D malic enzyme; oxaloacetate-decarboxylating; NAD-dependent; catalyzes the formation of pyruvate form malate; malate dehydrogenase 925689..927503 Francisella tularensis subsp. tularensis SCHU S4 3192386 YP_169915.1 CDS FTT_0918 NC_006570.2 927666 929339 D ORF ftt0918; hypothetical protein 927666..929339 Francisella tularensis subsp. tularensis SCHU S4 3191684 YP_169916.1 CDS FTT_0919 NC_006570.2 929356 930801 D ORF ftt0919; hypothetical protein 929356..930801 Francisella tularensis subsp. tularensis SCHU S4 3191682 YP_169917.1 CDS isftu1 NC_006570.2 930936 931720 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(930936..931262,931262..931720) Francisella tularensis subsp. tularensis SCHU S4 3190914 YP_169918.1 CDS FTT_0922 NC_006570.2 932888 933442 D Similarity to Q98JM3 Probable fatty acid hydroxylase from Rhizobium loti (164 aa). FASTA: opt: 305 Z-score: 383.8 E(): 1.6e-13 Smith-Waterman score: 305; 31.902 identity in 163 aa overlap. ORF ftt0922; fatty acid hydroxylase 932888..933442 Francisella tularensis subsp. tularensis SCHU S4 3191679 YP_169919.1 CDS rlmL NC_006570.2 933517 935670 D catalyzes the N2-methyl guanosine modification of the G2445 residue of 23S rRNA; 23S rRNA m(2)G2445 methyltransferase 933517..935670 Francisella tularensis subsp. tularensis SCHU S4 3192468 YP_169920.1 CDS FTT_0924 NC_006570.2 935772 936170 D ORF ftt0924; hypothetical protein 935772..936170 Francisella tularensis subsp. tularensis SCHU S4 3192465 YP_169921.1 CDS fmt NC_006570.2 936175 937116 D Similarity to P44787 Methionyl-tRNA formyltransferase from Haemophilus ducreyi (318 aa). FASTA: opt: 949 Z-score: 1086.2 E(): 1.2e-52 Smith-Waterman score: 958; 48.387 identity in 310 aa overlap; methionyl-tRNA formyltransferase 936175..937116 Francisella tularensis subsp. tularensis SCHU S4 3191926 YP_169922.1 CDS gshB NC_006570.2 937120 938094 D catalyzes the second step in the glutathione biosynthesis pathway, where it synthesizes ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione; glutathione synthetase 937120..938094 Francisella tularensis subsp. tularensis SCHU S4 3192106 YP_169923.1 CDS hemL NC_006570.2 938084 939379 D Converts (S)-4-amino-5-oxopentanoate to 5-aminolevulinate during the porphyrin biosynthesis pathway; glutamate-1-semialdehyde aminotransferase 938084..939379 Francisella tularensis subsp. tularensis SCHU S4 3192422 YP_169924.1 CDS FTT_0928c NC_006570.2 939400 941067 R SImilar to Q8RD12 Beta-glucosidase-related glycosidases from Thermoanaerobacter tengcongensis (591 aa). FASTA: opt: 1047 Z-score: 1141.5 E(): 9.9e-56 Smith-Waterman score: 1062; 35.106 identity in 564 aa overlap. ORF ftt0928c; beta-glucosidase complement(939400..941067) Francisella tularensis subsp. tularensis SCHU S4 3192464 YP_169925.1 CDS FTT_0931 NC_006570.2 942809 944068 D Similar to Q89YS8 Glucose/galactose transporter from Bacteroides thetaiotaomicron (415 aa). FASTA: opt: 1125 Z-score: 1176.3 bits: 226.6 E(): 1.3e-57 Smith-Waterman score: 1125; 41.446 identity in 415 aa overlap ORF ftt0931; major facilitator transporter 942809..944068 Francisella tularensis subsp. tularensis SCHU S4 3192459 YP_169926.1 CDS FTT_0932 NC_006570.2 944103 944954 D Similar to Q8A1Q9 ROK family transcriptional repressor from Bacteroides thetaiotaomicron (298 aa). FASTA: opt: 482 Z-score: 605.6 E(): 7.7e-26 Smith-Waterman score: 482; 30.769 identity in 286 aa overlap ORF ftt0932; ROK family protein 944103..944954 Francisella tularensis subsp. tularensis SCHU S4 3192460 YP_169927.1 CDS bioD NC_006570.2 945880 946557 R Similar to AAO90524 Dethiobiotin synthetase from Coxiella burnetii (242 aa). FASTA: opt: 464 Z-score: 551.2 E(): 7.5e-23 Smith-Waterman score: 464; 34.498 identity in 229 aa overlap; dethiobiotin synthetase complement(945880..946557) Francisella tularensis subsp. tularensis SCHU S4 3190963 YP_169928.1 CDS bioC NC_006570.2 946561 947298 R Similar to AAO90525 Biotin synthesis protein BioC, from Coxiella burnetii (248 aa) FASTA: opt: 232 Z-score: 273.5 E(): 2.2e-07 Smith-Waterman score: 232; 23.293 identity in 249 aa overlap; biotin synthesis protein BioC complement(946561..947298) Francisella tularensis subsp. tularensis SCHU S4 3190965 YP_169929.1 CDS bioF NC_006570.2 947276 948403 R Similar to AAO90527 8-amino-7-oxononanoate synthase (384 aa) fasta scores; opt: 763 Z-score: 912.3 E(): 5.8e-43 Smith-Waterman score: 763; 39.053identity in 338 aa overlap; 8-amino-7-oxononanoate synthase complement(947276..948403) Francisella tularensis subsp. tularensis SCHU S4 3190975 YP_169930.1 CDS bioB NC_006570.2 948397 949338 R Similar to AAO90528 Biotin synthase from Coxiella burnetii (321 aa) FASTA: opt: 1220 Z-score: 1430.1 E(): 8.3e-72 Smith-Waterman score: 1220; 60.396 identity in 303 aa overlap; biotin synthase complement(948397..949338) Francisella tularensis subsp. tularensis SCHU S4 3190968 YP_169931.1 CDS bioA NC_006570.2 949384 950715 D Similar to AAO90529 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase from Coxiella burnetii (442 aa). FASTA: opt: 1486 Z-score: 1733.9 E(): 9.9e-89 Smith-Waterman score: 1486; 50.588 identity in 425 aa overlap; adenosylmethionine-8-amino-7-oxononanoate aminotransferase 949384..950715 Francisella tularensis subsp. tularensis SCHU S4 3192333 YP_169932.1 CDS add1 NC_006570.2 950825 951769 R catalyzes the formation of inosine from adenosine; adenosine deaminase complement(950825..951769) Francisella tularensis subsp. tularensis SCHU S4 3192404 YP_169933.1 CDS FTT_0940c NC_006570.2 951917 952417 R Similar to Q88CI3 Conserved hypothetical protein from Pseudomonas putida (160 aa). FASTA: opt: 297 Z-score: 384.8 E(): 1.4e-13 Smith-Waterman score: 297; 32.653 identity in 147 aa overlap ORF ftt0940c; hypothetical protein complement(951917..952417) Francisella tularensis subsp. tularensis SCHU S4 3192457 YP_169934.1 CDS FTT_0941c NC_006570.2 952433 953353 R Similar to Q97VW1 Lipase (lipP-1) from Sulfolobus solfataricus (305 aa). FASTA: opt: 488 Z-score: 622.4 E(): 8.9e-27 Smith-Waterman score: 488; 32.990 identity in 291 aa overlap. ORF ftt0941c; lipase/esterase complement(952433..953353) Francisella tularensis subsp. tularensis SCHU S4 3192456 YP_169935.1 CDS folK NC_006570.2 953366 954631 R Similar to Q821E2 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase/dihydropteroate synthase from Chlamydophila caviae (447 aa). FASTA: opt: 517 Z-score: 602.2 E(): 1.2e-25 Smith-Waterman score: 604; 30.664 identity in 437 aa overlap; 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase complement(953366..954631) Francisella tularensis subsp. tularensis SCHU S4 3191528 YP_169936.1 CDS folB NC_006570.2 954633 954986 R Similar to O84620 Probable dihydroneopterin aldolase (124 aa) fasta scores; opt: 191 Z-score: 262.4 E(): 9.2e-07 Smith-Waterman score: 191; 30.189identity in 106 aa overlap; dihydroneopterin aldolase complement(954633..954986) Francisella tularensis subsp. tularensis SCHU S4 3191941 YP_169937.1 CDS FTT_0944 NC_006570.2 955056 956438 D Similar to Q9CHK3 O-succinylbenzoic acid-CoA ligase from Lactococcus lactis (420 aa). opt: 373 Z-score: 423.0 E(): 1e-15 Smith-Waterman score: 434; 27.692 identity in 390 aa overlap ORF ftt0944; AMP-binding protein 955056..956438 Francisella tularensis subsp. tularensis SCHU S4 3192455 YP_169938.1 CDS FTT_0945 NC_006570.2 956431 958194 D Similar to Q8ESD8 Para-aminobenzoate synthase component II (585 aa) fasta scores; opt: 1108 Z-score: 1235.5 E(): 5.7e-61 Smith-Waterman score: 1108; 33.851 identity in 579 aa overlap. ORF ftt0945; chorismate binding family protein 956431..958194 Francisella tularensis subsp. tularensis SCHU S4 3192454 YP_169939.1 CDS trpG1 NC_006570.2 958196 958750 D Similarity to Q972A0 anthranilate synthase component from Sulfolobus tokodaii (193 aa). FASTA: opt: 499 Z-score: 620.3 E(): 1.1e-26 Smith-Waterman score: 499; 40.741 identity in 189 aa overlap. Closely related to pabA proteins as they possess a shared domain feature; anthranilate synthase subunit II 958196..958750 Francisella tularensis subsp. tularensis SCHU S4 3190985 YP_169940.1 CDS FTT_0948c NC_006570.2 959971 961035 R Similar to Q92QZ5 oxidoreductase protein from Rhizobium meliloti (347 aa). FASTA: opt: 1125 Z-score: 1304.5 E(): 8.2e-65 Smith-Waterman score: 1125; 50.140 identity in 357 aa overlap. pseudogene: flanked by pseudogenes ORF ftt0948c; aldo/keto reductase complement(959971..961035) Francisella tularensis subsp. tularensis SCHU S4 3192451 YP_169941.1 CDS folE NC_006570.2 963398 964018 R involved in the first step of tetrahydrofolate biosynthesis; catalyzes the formation of formate and 2-amino-4-hydroxy-6-(erythro-1,2, 3-trihydroxypropyl)dihydropteridine triphosphate from GTP and water; forms a homopolymer; GTP cyclohydrolase I complement(963398..964018) Francisella tularensis subsp. tularensis SCHU S4 3191935 YP_169942.1 CDS rhlE NC_006570.2 964145 965470 D Similar to Q8D6C6 Superfamily II DNA and RNA helicase from Vibrio vulnificus (509 aa). FASTA: opt: 1533 Z-score: 1649.9 E(): 4.8e-84 Smith-Waterman score: 1533; 56.036 identity in 439 aa overlap; ATP-dependent RNA helicase RhlE 964145..965470 Francisella tularensis subsp. tularensis SCHU S4 3191629 YP_169943.1 CDS FTT_0953c NC_006570.2 965467 967008 R Similar to Q9KTB5 Proton/peptide symporter family protein from Vibrio cholerae (514 aa). FASTA: opt: 1329 Z-score: 1443.3 E(): 1.5e-72 Smith-Waterman score: 1329; 42.629identity in 502 aa overlap. ORF ftt0953c; proton-dependent oligopeptide transport (POT) family protein complement(965467..967008) Francisella tularensis subsp. tularensis SCHU S4 3192447 YP_169944.1 CDS FTT_0954c NC_006570.2 967146 967466 R Similar to O29392 Hypothetical protein AF0869 from Archaeoglobus fulgidus (108 aa). FASTA: opt: 272 Z-score: 377.5 E(): 3.9e-13 Smith-Waterman score: 272; 42.056 identity in 107 aa overlap ORF ftt0954c; hypothetical protein complement(967146..967466) Francisella tularensis subsp. tularensis SCHU S4 3192448 YP_169945.1 CDS gor NC_006570.2 967477 968838 R catalyzes the reduction of 2 glutathione to glutathione disulfide; maintains high levels of reduced glutathione in the cytosol; involved in redox regulation and oxidative defense; glutathione reductase complement(967477..968838) Francisella tularensis subsp. tularensis SCHU S4 3192553 YP_169946.1 CDS FTT_0956c NC_006570.2 968876 969595 R Similar to AAO50751 (Q86L42) Hypothetical protein from Dictyostelium discoideum (239 aa). FASTA: opt: 229 Z-score: 273.3 E(): 2.3e-07 Smith-Waterman score: 253; 29.437 identity in 231 aa overlap ORF ftt0956c; hypothetical protein complement(968876..969595) Francisella tularensis subsp. tularensis SCHU S4 3192446 YP_169947.1 CDS FTT_0957c NC_006570.2 969595 970098 R Similar to Q83YE2 Shy8 from Streptomyces hygroscopicus (163 aa). FASTA: opt: 343 Z-score: 413.4 bits: 82.8 E(): 3.9e-15 Smith-Waterman score: 343; 36.420 identity in 162 aa overlap. ORF ftt0957c; hypothetical protein complement(969595..970098) Francisella tularensis subsp. tularensis SCHU S4 3192445 YP_169948.1 CDS FTT_0958c NC_006570.2 970101 970808 R Similar to Q9HWL9 Probable short-chain dehydrogenase from Pseudomonas aeruginosa (238 aa). FASTA: opt: 848 Z-score: 1032.8 E(): 1.1e-49 Smith-Waterman score: 848; 55.932 identity in 236 aa overlap ORF ftt0958c; short chain dehydrogenase complement(970101..970808) Francisella tularensis subsp. tularensis SCHU S4 3192443 YP_169949.1 CDS xthA NC_006570.2 970829 971617 R Similar to Q83EM1 Exodeoxyribonuclease III from Coxiella burnetii (259 aa). FASTA: opt: 991 Z-score: 1173.5 E(): 1.6e-57 Smith-Waterman score: 991; 54.789 identity in 261 aa overlap; exodeoxyribonuclease III complement(970829..971617) Francisella tularensis subsp. tularensis SCHU S4 3192119 YP_169950.1 CDS FTT_0960 NC_006570.2 971901 972884 D Simiar to Q8EAC2 Conserved hypothetical protein Shewanella oneidensis (371 aa). FASTA: opt: 607 Z-score: 717.9 E(): 3.9e-32 Smith-Waterman score: 607; 32.622 identity in 328 aa overlap ORF ftt0960; hypothetical protein 971901..972884 Francisella tularensis subsp. tularensis SCHU S4 3192444 YP_169951.1 CDS mdaB NC_006570.2 972892 973482 D Similar to P40717 Modulator of drug activity B from Escherichia coli (193 aa). FASTA: opt: 900 Z-score: 1129.6 E(): 4.5e-55 Smith-Waterman score: 900; 63.542 identity in 192 aa overlap.; modulator of drug activity B 972892..973482 Francisella tularensis subsp. tularensis SCHU S4 3191872 YP_169952.1 CDS FTT_0962 NC_006570.2 973598 974260 D Similar to Q87Z85 ThiJ/PfpI family protein from Pseudomonas syringae (225 aa). FASTA: opt: 570 Z-score: 665.3 E(): 3.3e-29 Smith-Waterman score: 570; 38.991 identity in 218 aa overlap ORF ftt0962; ThiJ/PfpI family protein 973598..974260 Francisella tularensis subsp. tularensis SCHU S4 3192441 YP_169953.1 CDS aroG NC_006570.2 974330 975442 R catalyzes the formation of 3-deoxy-D-arabino-hept-2-ulosonate 7 phosphate from phosphoenolpyruvate and D-erythrose 4-phosphate, phenylalanine sensitive; phospho-2-dehydro-3-deoxyheptonate aldolase complement(974330..975442) Francisella tularensis subsp. tularensis SCHU S4 3190950 YP_169954.1 CDS ffh NC_006570.2 975474 976850 R Similar to Q9CLN6 Ffh from Pasteurella multocida (458 aa). FASTA: opt: 1851 Z-score: 1789.9 E(): 7.5e-92 Smith-Waterman score: 1851; 61.790 identity in 458 aa overlap; signal recognition particle protein, Ffh complement(975474..976850) Francisella tularensis subsp. tularensis SCHU S4 3191883 YP_169955.1 CDS FTT_0965c NC_006570.2 977194 978099 R Similar to Q928M9 Hypothetical protein lin2503 from Listeria innocua (279 aa). FASTA: opt: 733 Z-score: 821.7 E(): 6.4e-38 Smith-Waterman score: 733; 41.852 identity in 270 aa overlap. ORF ftt0965c; hypothetical protein complement(977194..978099) Francisella tularensis subsp. tularensis SCHU S4 3192440 YP_169956.1 CDS infA NC_006570.2 978193 978411 D stimulates the activities of the other two initiation factors, IF-2 and IF-3; translation initiation factor IF-1 978193..978411 Francisella tularensis subsp. tularensis SCHU S4 3191552 YP_169957.1 CDS FTT_0968c NC_006570.2 979373 980788 R Similar to Q83BZ0 Amino acid antiporter from Coxiella burnetii (474 aa). FASTA: 432 opt: 1275 Z-score: 1361.2 E(): 6.3e-68 Smith-Waterman score: 1275; 40.171 identity in 468 aa overlap. ORF ftt0968c; amino acid antiporter complement(979373..980788) Francisella tularensis subsp. tularensis SCHU S4 3192433 YP_169958.1 CDS trkA NC_006570.2 980853 982226 R involved in potassium uptake; found to be peripherally associated with the inner membrane in Escherichia coli; contains an NAD-binding domain; potassium transporter peripheral membrane protein complement(980853..982226) Francisella tularensis subsp. tularensis SCHU S4 3191475 YP_169959.1 CDS FTT_0970 NC_006570.2 982379 982789 D Similar to Q8PIG4 Hypothetical protein XAC2934 from Xanthomonas axonopodis (152 aa). FASTA: opt: 281 Z-score: 360.3 E(): 3.2e-12 Smith-Waterman score: 281; 33.588 identity in 131 aa overlap ORF ftt0970; hypothetical protein 982379..982789 Francisella tularensis subsp. tularensis SCHU S4 3191852 YP_169960.1 CDS FTT_0971 NC_006570.2 982796 984241 D with SufCD activates cysteine desulfurase SufS; cysteine desulfurase 982796..984241 Francisella tularensis subsp. tularensis SCHU S4 3190927 YP_169961.1 CDS FTT_0972 NC_006570.2 984280 985029 D Similar to Q8PIG2 ABC transporter ATP-binding protein from Xanthomonas axonopodis (254 aa). FASTA: opt: 1080 Z-score: 1117.5 E(): 2.1e-54 Smith-Waterman score: 1080; 65.041 identity in 246 aa overlap. ORF ftt0972; ABC transporter ATP-binding protein 984280..985029 Francisella tularensis subsp. tularensis SCHU S4 3190906 YP_169962.1 CDS FTT_0973 NC_006570.2 985033 986187 D Similar to Q83BX9 Hypothetical protein CBU1358 Coxiella burnetii (426 aa). FASTA: opt: 433 Z-score: 493.2 E(): 1.3e-19 Smith-Waterman score: 460; 29.282 identity in 362 aa overlap ORF ftt0973; hypothetical protein 985033..986187 Francisella tularensis subsp. tularensis SCHU S4 3190904 YP_169963.1 CDS FTT_0975 NC_006570.2 987156 987845 D Weakly similar to Q83CZ8 Hypothetical protein Coxiella burnetii (227 aa) 76 opt: 235 Z-score: 276.0 bits: 58.3 E(): 1.8e-07 Smith-Waterman score: 235; 25.000 identity in 236 aa overlap. ORF ftt0975; hypothetical protein 987156..987845 Francisella tularensis subsp. tularensis SCHU S4 3190902 YP_169964.1 CDS trxA2 NC_006570.2 987849 988175 D Similar to P37395 Thioredoxin from Cyanidium caldarium (107 aa) fasta scores; opt: 333 Z-score: 452.0 E(): 2.5e-17 Smith-Waterman score: 333; 45.098identity in 102 aa overlap; thioredoxin 987849..988175 Francisella tularensis subsp. tularensis SCHU S4 3191003 YP_169965.1 CDS FTT_0977c NC_006570.2 988574 988867 R Similar to Q89DD5 Bsl7504 protein from Bradyrhizobium japonicum (99 aa). FASTA: opt: 209 Z-score: 292.0 E(): 2e-08 Smith-Waterman score: 209; 38.462 identity in 91 aa overlap ORF ftt0977c; hypothetical protein complement(988574..988867) Francisella tularensis subsp. tularensis SCHU S4 3190901 YP_169966.1 CDS FTT_0978c NC_006570.2 988871 989476 R ORF ftt0978c; hypothetical protein complement(988871..989476) Francisella tularensis subsp. tularensis SCHU S4 3190898 YP_169967.1 CDS FTT_0979c NC_006570.2 989530 990897 R amino-acid permease, similarity to P39137 Amino-acid permease rocE from Bacillus subtilis (467 aa). FASTA: opt: 1272 Z-score: 1431.9 E(): 6.6e-72 Smith-Waterman score: 1272; 40.919 identity in 457 aa overlap. ORF ftt0979c; amino-acid permease complement(989530..990897) Francisella tularensis subsp. tularensis SCHU S4 3190899 YP_169968.1 CDS FTT_0980 NC_006570.2 991132 992373 D Similar to Q8UKI4 Aminotransferase, class II from Agrobacterium tumefaciens (413 aa). FASTA: opt: 486 Z-score: 559.2 E(): 2.7e-23 Smith-Waterman score: 554; 28.493 identity in 365 aa overlap ORF ftt0980; hypothetical protein 991132..992373 Francisella tularensis subsp. tularensis SCHU S4 3190897 YP_169969.1 CDS FTT_0981 NC_006570.2 992706 993836 D Weak homology to two-component sensory protein. ORF ftt0981; hypothetical protein 992706..993836 Francisella tularensis subsp. tularensis SCHU S4 3190896 YP_169970.1 CDS FTT_0982 NC_006570.2 993929 994072 D ORF ftt0982; hypothetical protein 993929..994072 Francisella tularensis subsp. tularensis SCHU S4 3190895 YP_169972.1 CDS mutT NC_006570.2 994786 995196 R Similar to Q87SF6 Mutator MutT protein from Vibrio parahaemolyticus (132 aa). FASTA: opt: 268 Z-score: 340.9 E(): 3.9e-11 Smith-Waterman score: 268; 29.545identity in 132 aa overlap.; Mutator protein complement(994786..995196) Francisella tularensis subsp. tularensis SCHU S4 3192063 YP_169973.1 CDS FTT_0985 NC_006570.2 995235 995819 D Similar to Q8EBZ9 Conserved hypothetical protein from Shewanella oneidensis (187 aa). FASTA: opt: 386 Z-score: 477.0 E(): 1e-18 Smith-Waterman score: 386; 36.022 identity in 186 aa overlap ORF ftt0985; hypothetical protein 995235..995819 Francisella tularensis subsp. tularensis SCHU S4 3190893 YP_169974.1 CDS FTT_0986 NC_006570.2 995821 996231 D Similar to Q9L3I7 Holliday junction resolvase from Aeromonas hydrophila (140 aa). FASTA: opt: 336 Z-score: 430.0 E(): 4.7e-16 Smith-Waterman score: 336; 36.567 identity in 134 aa overlap Holliday junction resolvase predicted in other proteins ORF ftt0986; hypothetical protein 995821..996231 Francisella tularensis subsp. tularensis SCHU S4 3190892 YP_169975.1 CDS FTT_0987 NC_006570.2 996324 996521 D ORF ftt0987; hypothetical protein 996324..996521 Francisella tularensis subsp. tularensis SCHU S4 3190891 YP_169976.1 CDS FTT_0988 NC_006570.2 996644 996832 D ORF ftt0988; hypothetical protein 996644..996832 Francisella tularensis subsp. tularensis SCHU S4 3190889 YP_169977.1 CDS FTT_0989 NC_006570.2 996926 998821 D Similar to Q9I271 Hypothetical protein PA2044 from Pseudomonas aeruginosa (624 aa). FASTA: opt: 447 Z-score: 484.0 E(): 4.2e-19 Smith-Waterman score: 447; 22.203 identity in 572 aa overlap. ORF ftt0989; hypothetical protein 996926..998821 Francisella tularensis subsp. tularensis SCHU S4 3190890 YP_169978.1 CDS leuS NC_006570.2 998929 1001370 D leucine--tRNA ligase; LeuRS; class-I aminoacyl-tRNA synthetase; charges leucine by linking carboxyl group to alpha-phosphate of ATP and then transfers aminoacyl-adenylate to its tRNA; due to the large number of codons that tRNA(Leu) recognizes, the leucyl-tRNA synthetase does not recognize the anticodon loop of the tRNA, but instead recognition is dependent on a conserved discriminator base A37 and a long arm; an editing domain hydrolyzes misformed products; in Methanothermobacter thermautotrophicus this enzyme associates with prolyl-tRNA synthetase; leucyl-tRNA synthetase 998929..1001370 Francisella tularensis subsp. tularensis SCHU S4 3190983 YP_169979.1 CDS FTT_0991 NC_006570.2 1001378 1001953 D ORF ftt0991; lipoprotein 1001378..1001953 Francisella tularensis subsp. tularensis SCHU S4 3190888 YP_169980.1 CDS FTT_0992 NC_006570.2 1002014 1003204 D Similar to Q8A7A2 membrane protein from Bacteroides thetaiotaomicron (391 aa). FASTA: opt: 908 Z-score: 1062.3 E(): 2.6e-51 Smith-Waterman score: 908; 37.637 identity in 364 aa overlap. ORF ftt0992; hypothetical protein 1002014..1003204 Francisella tularensis subsp. tularensis SCHU S4 3190887 YP_169981.1 CDS dcd NC_006570.2 1003211 1003777 R Catalyzes the formation of dUTP from dCTP in thymidylate biosynthesis; deoxycytidine triphosphate deaminase complement(1003211..1003777) Francisella tularensis subsp. tularensis SCHU S4 3191500 YP_169982.1 CDS FTT_0994c NC_006570.2 1003789 1004649 R Similar to Q8XWU1 Probable MRP protein ATP-binding from Ralstonia solanacearum. (362 aa). FASTA: opt: 1008 Z-score: 1105.3 E(): 1e-53 Smith-Waterman score: 1008; 52.688 identity in 279 aa overlap. ORF ftt0994c; MRP like protein complement(1003789..1004649) Francisella tularensis subsp. tularensis SCHU S4 3190886 YP_169983.1 CDS FTT_0995 NC_006570.2 1004723 1005955 D Similarity to Q8UDH1 MFS permease[proline/betaine] from Agrobacterium tumefaciens (443 aa). FASTA: opt: 727 Z-score: 824.0 E(): 4.8e-38 Smith-Waterman score: 828; 35.507identity in 414 aa overlap ORF ftt0995; major facilitator transporter 1004723..1005955 Francisella tularensis subsp. tularensis SCHU S4 3190885 YP_169984.1 CDS ybhO NC_006570.2 1007525 1008955 D Similar to P71040 Probable cardiolipin synthetase 2 from Bacillus subtilis (482 aa). FASTA: opt: 835 Z-score: 944.7 E(): 9.1e-45 Smith-Waterman score: 835; 32.525 identity in 495 aa overlap; cardiolipin synthetase 1007525..1008955 Francisella tularensis subsp. tularensis SCHU S4 3192157 YP_169985.1 CDS FTT_0998 NC_006570.2 1009121 1010110 D ORF ftt0998; hypothetical protein 1009121..1010110 Francisella tularensis subsp. tularensis SCHU S4 3192073 YP_169986.1 CDS cobS NC_006570.2 1010878 1012104 R Similar to Q8FUU8 Cobalamin synthesis protein/P47K family protein from Brucella suis (400 aa). FASTA: opt: 1643 Z-score: 1956.7 E(): 4.3e-101 Smith-Waterman score: 1643; 58.396 identity in 399 aa overlap.; cobalamin (vitamin B12) synthesis protein/P47K family protein complement(1010878..1012104) Francisella tularensis subsp. tularensis SCHU S4 3191886 YP_169987.1 CDS FTT_1001 NC_006570.2 1012246 1012677 D Similar to Q8ZJ15 regulatory protein from Yersinia pestis (169 aa). FASTA: opt: 313 Z-score: 406.8 E(): 9.2e-15 Smith-Waterman score: 313; 36.429 identity in 140 aa overlap. ORF ftt1001; regulatory protein 1012246..1012677 Francisella tularensis subsp. tularensis SCHU S4 3192069 YP_169988.1 CDS pheT NC_006570.2 1012720 1015092 R catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a tetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 2 subfamily; phenylalanyl-tRNA synthetase subunit beta complement(1012720..1015092) Francisella tularensis subsp. tularensis SCHU S4 3191133 YP_169989.1 CDS pheS NC_006570.2 1015113 1016126 R catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a heterotetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 1 subfamily; phenylalanyl-tRNA synthetase subunit alpha complement(1015113..1016126) Francisella tularensis subsp. tularensis SCHU S4 3191372 YP_169990.1 CDS FTT_1004c NC_006570.2 1016212 1017105 R Similar to Q9RX03 Hypothetical protein DR0512 from Deinococcus radiodurans (301 aa). Similar to opt: 577 Z-score: 644.6 E(): 5.1e-28 Smith-Waterman score: 577; 33.094 identity in 278 aa overlap. ORF ftt1004c; hypothetical protein complement(1016212..1017105) Francisella tularensis subsp. tularensis SCHU S4 3192070 YP_169991.1 CDS yhiP NC_006570.2 1017130 1018605 R Similar to Q8X5Q8 transport protein from Escherichia coli (489 aa). FASTA: opt: 1050 Z-score: 1137.7 E(): 1.8e-55 Smith-Waterman score: 1050; 35.569 identity in 492 aa overlap.; proton-dependent oligopeptide transporter (POT) family protein complement(1017130..1018605) Francisella tularensis subsp. tularensis SCHU S4 3191273 YP_008492912.1 CDS FTT_0005a NC_006570.2 1018628 1018813 R ABC transporter ATP-binding protein complement(1018628..1018813) Francisella tularensis subsp. tularensis SCHU S4 16744728 YP_169992.1 CDS FTT_1006 NC_006570.2 1018849 1020057 D ORF ftt1006; hypothetical protein 1018849..1020057 Francisella tularensis subsp. tularensis SCHU S4 3192068 YP_169995.1 CDS FTT_1009 NC_006570.2 1021117 1021653 D ORF ftt1009; hypothetical protein 1021117..1021653 Francisella tularensis subsp. tularensis SCHU S4 3192064 YP_169996.1 CDS FTT_1010 NC_006570.2 1021668 1021871 D Similar to Q81BZ8 Transcriptional regulator,Cro/CI family protein from Bacillus cereus (strain ATCC 14579 / DSM 31) (71 aa). FASTA: opt: 263 Z-score: 391.6 E(): 6.4e-14 Smith-Waterman score: 263; 60.606 identity in 66 aa overlap. ORF ftt1010; helix-turn-helix family protein 1021668..1021871 Francisella tularensis subsp. tularensis SCHU S4 3192061 YP_169997.1 CDS FTT_1011 NC_006570.2 1021983 1022171 D ORF ftt1011; hypothetical protein 1021983..1022171 Francisella tularensis subsp. tularensis SCHU S4 3192062 YP_169998.1 CDS ruvB NC_006570.2 1023158 1024204 R promotes strand exchange during homologous recombination; RuvAB complex promotes branch migration; RuvABC complex scans the DNA during branch migration and resolves Holliday junctions at consensus sequences; forms hexameric rings around opposite DNA arms; requires ATP for branch migration and orientation of RuvAB complex determines direction of migration; Holliday junction DNA helicase RuvB complement(1023158..1024204) Francisella tularensis subsp. tularensis SCHU S4 3191291 YP_169999.1 CDS FTT_1014c NC_006570.2 1024268 1024993 R Similar to YWFD_BACSU (P39640) Hypothetical oxidoreductase ywfD from Bacillus subtilis (255 aa). FASTA: opt: 410 Z-score: 474.7 E(): 1.5e-18 Smith-Waterman score: 471; 38.710 identity in 248 aa overlap. ORF ftt1014c; short chain dehydrogenase family protein complement(1024268..1024993) Francisella tularensis subsp. tularensis SCHU S4 3192059 YP_170000.1 CDS FTT_1015 NC_006570.2 1025111 1025701 D ORF ftt1015; hypothetical protein 1025111..1025701 Francisella tularensis subsp. tularensis SCHU S4 3192056 YP_170001.1 CDS FTT_1016c NC_006570.2 1025711 1026340 R Similar to Q9JQY7 lipase (GDSL lipase family protein) from Neisseria meningitidis (213 aa). FASTA: opt: 358 Z-score: 439.3 E(): 1.4e-16 Smith-Waterman score: 358; 32.203 identity in 177 aa overlap. ORF ftt1016c; GDSL-like lipase/acylhydrolase family protein complement(1025711..1026340) Francisella tularensis subsp. tularensis SCHU S4 3192058 YP_170002.1 CDS FTT_1017c NC_006570.2 1026362 1026697 R ORF ftt1017c; hypothetical protein complement(1026362..1026697) Francisella tularensis subsp. tularensis SCHU S4 3192055 YP_170003.1 CDS dxs NC_006570.2 1026713 1028560 R catalyzes the formation of 1-deoxy-D-xylulose 5-phosphate from pyruvate and D-glyceraldehyde 3-phosphate; 1-deoxy-D-xylulose-5-phosphate synthase complement(1026713..1028560) Francisella tularensis subsp. tularensis SCHU S4 3191556 YP_170004.1 CDS guaA NC_006570.2 1028642 1030192 R contains glutamine-hydrolyzing domain and glutamine amidotransferase; GMP-binding domain; functions to produce GMP from XMP in the IMP pathway; GMP synthase complement(1028642..1030192) Francisella tularensis subsp. tularensis SCHU S4 3191098 YP_170005.1 CDS FTT_1020c NC_006570.2 1030280 1031827 R Similar to Q83EA0 Amino acid permease family protein from Coxiella burnetii (531 aa). FASTA: opt: 925 Z-score: 996.3 E(): 1.3e-47 Smith-Waterman score: 947; 29.379 identity in 531 aa overlap. ORF ftt1020c; amino acid permease complement(1030280..1031827) Francisella tularensis subsp. tularensis SCHU S4 3192053 YP_170006.1 CDS truA NC_006570.2 1031880 1032656 R mediates pseudouridylation (positions 38, 39, 40) at the tRNA anticodon region which contributes to the structural stability; tRNA pseudouridine synthase A complement(1031880..1032656) Francisella tularensis subsp. tularensis SCHU S4 3190996 YP_170007.1 CDS FTT_1022c NC_006570.2 1032659 1034491 R Similar to Y125_PYRHO (O57865) Hypothetical protein PH0125 from Pyrococcus horikoshii (257 aa) (from 1-260). FASTA: opt: 520 Z-score: 632.1 E(): 2.6e-27 Smith-Waterman score: 520; 35.600 identity in 250 aa overlap AND Q97VT8 Lipase (lipP-2) from Sulfolobus solfataricus (311 aa) (from 300-610). FASTA: opt: 613 Z-score: 743.7 E(): 1.6e-33 Smith-Waterman score: 613; 34.586 identity in 266 aa overlap Possible merged protein due to a missing stop codon between 2 CDSs? ORF ftt1022c; hypothetical protein complement(1032659..1034491) Francisella tularensis subsp. tularensis SCHU S4 3192052 YP_170008.1 CDS FTT_1023c NC_006570.2 1034504 1035097 R Similar to Q8EGL6 Fumarylacetoacetate hydrolase family protein from Shewanella oneidensis (208 aa). FASTA: opt: 599 Z-score: 746.5 E(): 1.1e-33 Smith-Waterman score: 599; 51.913 identity in 183 aa overlap. ORF ftt1023c; fumarylacetoacetate hydrolase family protein complement(1034504..1035097) Francisella tularensis subsp. tularensis SCHU S4 3192050 YP_170009.1 CDS yhbG NC_006570.2 1035146 1035877 R Similar to Q8ZB44 Probable ABC transporter ATP-binding protein from Yersinia pestis (241 aa). FASTA: opt: 1091 Z-score: 1233.5 E(): 8.2e-61 Smith-Waterman score: 1091; 65.690 identity in 239 aa overlap.; ABC transporter ATP-binding protein complement(1035146..1035877) Francisella tularensis subsp. tularensis SCHU S4 3192168 YP_170010.1 CDS FTT_1025c NC_006570.2 1035951 1036784 R ORF ftt1025c; hypothetical protein complement(1035951..1036784) Francisella tularensis subsp. tularensis SCHU S4 3192048 YP_170011.1 CDS FTT_1026c NC_006570.2 1036771 1037397 R ORF ftt1026c; hypothetical protein complement(1036771..1037397) Francisella tularensis subsp. tularensis SCHU S4 3192049 YP_170012.1 CDS yrbI NC_006570.2 1037405 1037953 R Similar to AAP18518 Hypothetical protein yrbI from Escherichia coli (188 aa). FASTA: initn: 454 init1: 247 opt: 453 Z-score: 558.6 bits: 110.0 E(): 3.2e-23 Smith-Waterman score: 453; 50.000 identity in 152 aa overlap.; 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase complement(1037405..1037953) Francisella tularensis subsp. tularensis SCHU S4 3192403 YP_170013.1 CDS ppa NC_006570.2 1038044 1038565 R Similar to IPYR_YERPE (Q8ZB98) Inorganic pyrophosphatase from Yersinia pestis (175 aa). FASTA: opt: 762 Z-score: 1001.4 E(): 6.9e-48 Smith-Waterman score: 762; 62.209 identity in 172 aa overlap.; inorganic pyrophosphatase complement(1038044..1038565) Francisella tularensis subsp. tularensis SCHU S4 3191284 YP_170014.1 CDS dacD NC_006570.2 1038722 1040056 D Similar to Q87VW4 D-alanyl-D-alanine carboxypeptidase from Pseudomonas syringae (pv. tomato) (386 aa). FASTA: opt: 602 Z-score: 704.0 E(): 2.5e-31 Smith-Waterman score: 794; 36.409 identity in 401 aa overlap.; D-alanyl-D-alanine carboxypeptidase 1038722..1040056 Francisella tularensis subsp. tularensis SCHU S4 3191499 YP_170015.1 CDS FTT_1030 NC_006570.2 1040089 1040373 D Similar to YE87_NITEU (Q82UJ7) Hypothetical UPF0250 protein NE1487 from Nitrosomonas europaea (87 aa). FASTA: opt: 235 Z-score: 328.5 E(): 2.1e-10 Smith-Waterman score: 235; 36.471 identity in 85 aa overlap. ORF ftt1030; hypothetical protein 1040089..1040373 Francisella tularensis subsp. tularensis SCHU S4 3192047 YP_170016.1 CDS lipB NC_006570.2 1040383 1041003 D lipoyl/octanoyltransferase; catalyzes the transfer of the lipoyl/octanoyl moiety of lipoyl/octanoyl-ACP onto lipoate-dependent enzymes like pyruvate dehydrogenase and the glycine cleavage system H protein; lipoate-protein ligase B 1040383..1041003 Francisella tularensis subsp. tularensis SCHU S4 3190841 YP_170017.1 CDS ndh NC_006570.2 1045072 1046334 R Similar to Q8UDU6 NADH dehydrogenase from Agrobacterium tumefaciens (strain C58 / ATCC 33970) (438 aa). FASTA: opt: 826 Z-score: 983.7 E(): 6.7e-47 Smith-Waterman score: 826; 32.854 identity in 417 aa overlap.; NADH dehydrogenase complement(1045072..1046334) Francisella tularensis subsp. tularensis SCHU S4 3191685 YP_170018.1 CDS rpoD NC_006570.2 1046441 1048174 R Similar to P77890 LprpoD (RNA polymerase sigma factor) from Legionella pneumophila (620 aa). FASTA: opt: 1367 Z-score: 1480.9 E(): 1.4e-74 Smith-Waterman score: 1624; 47.182 identity in 621 aa overlap.; RNA polymerase sigma-70 factor complement(1046441..1048174) Francisella tularensis subsp. tularensis SCHU S4 3191979 YP_170019.1 CDS dnaG NC_006570.2 1048194 1050011 R Similar to Q8DEG2 DNA primase from Vibrio vulnificus (587 aa). FASTA: opt: 1152 Z-score: 1324.3 E(): 7.2e-66 Smith-Waterman score: 1155; 39.679 identity in 499 aa overlap.; DNA primase complement(1048194..1050011) Francisella tularensis subsp. tularensis SCHU S4 3191526 YP_170020.1 CDS FTT_1037c NC_006570.2 1050032 1050478 R Similar to Q87SL6 Conserved hypothetical protein from Vibrio parahaemolyticus (147 aa). FASTA: opt: 473 Z-score: 548.0 E(): 1.2e-22 Smith-Waterman score: 473; 47.586 identity in 145 aa overlap. ORF ftt1037c; hypothetical protein complement(1050032..1050478) Francisella tularensis subsp. tularensis SCHU S4 3192045 YP_170021.1 CDS rpsU NC_006570.2 1050539 1050736 R a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA; 30S ribosomal protein S21 complement(1050539..1050736) Francisella tularensis subsp. tularensis SCHU S4 3191008 YP_170022.1 CDS dacB1 NC_006570.2 1050992 1052395 D Similar to Q83FD1 D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase from Coxiella burnetii (477 aa). FASTA: opt: 654 Z-score: 780.3 E(): 1.4e-35 Smith-Waterman score: 654; 29.436 identity in 479 aa overlap.; D-alanyl-D-alanine carboxypeptidase 1050992..1052395 Francisella tularensis subsp. tularensis SCHU S4 3191495 YP_170023.1 CDS FTT_1040 NC_006570.2 1052396 1053007 D Similar to Q8EBG1 Conserved hypothetical protein from Shewanella oneidensis (205 aa). FASTA: opt: 610 Z-score: 776.5 E(): 2.3e-35 Smith-Waterman score: 610; 45.810 identity in 179 aa overlap. ORF ftt1040; lipoprotein 1052396..1053007 Francisella tularensis subsp. tularensis SCHU S4 3192043 YP_170024.1 CDS FTT_1041 NC_006570.2 1053023 1053439 D Similar to Q8ESG1 Hypothetical conserved protein from Oceanobacillus iheyensis (134 aa). FASTA: opt: 215 Z-score: 303.3 E(): 5.3e-09 Smith-Waterman score: 215; 32.773 identity in 119 aa overlap. ORF ftt1041; hypothetical protein 1053023..1053439 Francisella tularensis subsp. tularensis SCHU S4 3192044 YP_170025.1 CDS FTT_1042 NC_006570.2 1053432 1054235 D TtcA; YdaO; catalyzes the thiolation of cytosine 32 in specific tRNAs; C32 tRNA thiolase 1053432..1054235 Francisella tularensis subsp. tularensis SCHU S4 3192041 YP_170026.1 CDS FTT_1043 NC_006570.2 1054249 1055061 D Similar to O32766 Macrophage infectivity potentiator from Legionella hackeliae (249 aa). FASTA: opt: 235 Z-score: 273.3 E(): 2.5e-07 Smith-Waterman score: 343; 27.599 identity in 279 aa overlap. ORF ftt1043; FKBP-type peptidylprolyl isomerase 1054249..1055061 Francisella tularensis subsp. tularensis SCHU S4 3192042 YP_170027.1 CDS ispB NC_006570.2 1055079 1056056 R Similar to Q8EB79 Octaprenyl-diphosphate synthase from Shewanella oneidensis (323 aa). FASTA: opt: 1117 Z-score: 1360.2 E(): 7.1e-68 Smith-Waterman score: 1117; 53.704 identity in 324 aa overlap.; octaprenyl-diphosphate synthase complement(1055079..1056056) Francisella tularensis subsp. tularensis SCHU S4 3190799 YP_170028.1 CDS FTT_1045c NC_006570.2 1056146 1056577 R ORF ftt1045c; hypothetical protein complement(1056146..1056577) Francisella tularensis subsp. tularensis SCHU S4 3192040 YP_170029.1 CDS FTT_1046c NC_006570.2 1056658 1057281 R ORF ftt1046c; hypothetical protein complement(1056658..1057281) Francisella tularensis subsp. tularensis SCHU S4 3192039 YP_170030.2 CDS FTT_1047c NC_006570.2 1057953 1058315 R ORF ftt1047c; hypothetical protein complement(1057953..1058315) Francisella tularensis subsp. tularensis SCHU S4 3192038 YP_170031.2 CDS FTT_1048c NC_006570.2 1058367 1058933 R ORF ftt1048c; hypothetical protein complement(1058367..1058933) Francisella tularensis subsp. tularensis SCHU S4 3192037 YP_170032.1 CDS cysN NC_006570.2 1059979 1061391 R Similar to CYSN_VIBPA (Q87SX9) Sulfate adenylyltransferase subunit 1 from Vibrio parahaemolyticus (476 aa). FASTA: opt: 1823 Z-score: 2170.8 E(): 5e-113 Smith-Waterman score: 1823; 59.488 identity in 469 aa overlap.; sulfate adenylyltransferase complement(1059979..1061391) Francisella tularensis subsp. tularensis SCHU S4 3191498 YP_170033.1 CDS FTT_1051c NC_006570.2 1061830 1061994 R ORF ftt1051c; hypothetical protein complement(1061830..1061994) Francisella tularensis subsp. tularensis SCHU S4 3192036 YP_170034.1 CDS FTT_1052c NC_006570.2 1062454 1062660 R ORF ftt1052c; hypothetical protein complement(1062454..1062660) Francisella tularensis subsp. tularensis SCHU S4 3192035 YP_170035.1 CDS rimI NC_006570.2 1064388 1064876 R Similar to RIMI_ECOLI (P09453) Ribosomal-protein-alanine acetyltransferase (148 aa). FASTA: opt: 237 Z-score: 305.5 E(): 4e-09 Smith-Waterman score: 237; 36.585 identity in 123 aa overlap.; ribosomal-protein-alanine acetyltransferase complement(1064388..1064876) Francisella tularensis subsp. tularensis SCHU S4 3191621 YP_170036.1 CDS FTT_1055c NC_006570.2 1064854 1065279 R ORF ftt1055c; hypothetical protein complement(1064854..1065279) Francisella tularensis subsp. tularensis SCHU S4 3192031 YP_170037.1 CDS rluB NC_006570.2 1065279 1066094 R Similar to Q8ZEG0 Ribosomal large subunit pseudouridine synthase B from Yersinia pestis (318 aa). FASTA: opt: 938 Z-score: 1164.9 E(): 5.4e-57 Smith-Waterman score: 938; 56.705identity in 261 aa overlap.; ribosomal large subunit pseudouridine synthase B complement(1065279..1066094) Francisella tularensis subsp. tularensis SCHU S4 3191617 YP_170038.1 CDS FTT_1057c NC_006570.2 1066087 1066992 R Similar to Q9KRM4 Fimbrial biogenesis and twitching motility protein, from Vibrio cholerae (237 aa). FASTA: opt: 249 Z-score: 293.4 E(): 1.9e-08 Smith-Waterman score: 249; 25.346 identity in 217 aa overlap. Tetratrico peptide repeat (TPR) containing protein. Shows a weak similarity to the lipoprotein pilF (Pseudomonas) that has an N-terminal type II (lipoprotein) signal sequence, followed by six tandem degenerated 34-amino acid repeats, which are involved in protein-protein interactions. ORF ftt1057c; Type IV pili lipoprotein complement(1066087..1066992) Francisella tularensis subsp. tularensis SCHU S4 3192032 YP_170039.1 CDS FTT_1058c NC_006570.2 1066982 1068094 R Similar to Y2A6_PSEAE (Q51385) Hypothetical protein PA3806 from Pseudomonas aeruginosa (379 aa). FASTA: opt: 1458 Z-score: 1752.5 E(): 1e-89 Smith-Waterman score: 1458; 60.434 identity in 369 aa overlap. ORF ftt1058c; radical SAM superfamily protein complement(1066982..1068094) Francisella tularensis subsp. tularensis SCHU S4 3192030 YP_170040.1 CDS dnaB NC_006570.2 1068125 1069522 R Similar to DNAB_PASMU (Q9CNL6) Replicative DNA helicase from Pasteurella multocida (467 aa). FASTA: opt: 1484 Z-score: 1719.2 E(): 7.2e-88 Smith-Waterman score: 1484; 51.016 identity in 443 aa overlap.; replicative DNA helicase complement(1068125..1069522) Francisella tularensis subsp. tularensis SCHU S4 3191525 YP_170041.1 CDS rplI NC_006570.2 1069610 1070065 R in Escherichia coli this protein is wrapped around the base of the L1 stalk; 50S ribosomal protein L9 complement(1069610..1070065) Francisella tularensis subsp. tularensis SCHU S4 3192016 YP_170042.1 CDS rpsR NC_006570.2 1070084 1070302 R binds as a heterodimer with protein S6 to the central domain of the 16S rRNA; helps stabilize the platform of the 30S subunit; 30S ribosomal protein S18 complement(1070084..1070302) Francisella tularensis subsp. tularensis SCHU S4 3192527 YP_170043.1 CDS rpsF NC_006570.2 1070318 1070653 R binds cooperatively with S18 to the S15-16S complex, allowing platform assembly to continue with S11 and S21; 30S ribosomal protein S6 complement(1070318..1070653) Francisella tularensis subsp. tularensis SCHU S4 3191971 YP_170044.1 CDS hemF NC_006570.2 1071331 1072257 D catalyzes the conversion of the propionic acid groups of rings I and III to vinyl groups during heme synthesis; coproporphyrinogen III oxidase 1071331..1072257 Francisella tularensis subsp. tularensis SCHU S4 3192419 YP_170046.1 CDS isftu1 NC_006570.2 1073411 1074195 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(1073411..1073869,1073869..1074195)) Francisella tularensis subsp. tularensis SCHU S4 3192478 YP_170047.1 CDS FTT_1066c NC_006570.2 1074364 1074738 R ORF ftt1066c; hypothetical protein complement(1074364..1074738) Francisella tularensis subsp. tularensis SCHU S4 3191073 YP_170048.1 CDS FTT_1068c NC_006570.2 1078054 1078632 R ORF ftt1068c; hypothetical protein complement(1078054..1078632) Francisella tularensis subsp. tularensis SCHU S4 3192027 YP_170049.1 CDS FTT_1069c NC_006570.2 1078721 1079626 R ORF ftt1069c; hypothetical protein complement(1078721..1079626) Francisella tularensis subsp. tularensis SCHU S4 3192288 YP_170050.1 CDS FTT_1071c NC_006570.2 1082895 1083401 R Similar to Q82XK5 antirestriction protein from Nitrosomonas europaea (171 aa). FASTA: opt: 426 Z-score: 519.1 E(): 5e-21 Smith-Waterman score: 426; 42.771 identity in 166 aa overlap. ORF ftt1071c; hypothetical protein complement(1082895..1083401) Francisella tularensis subsp. tularensis SCHU S4 3190794 YP_170051.1 CDS FTT_1072 NC_006570.2 1084045 1084674 D ORF ftt1072; hypothetical protein 1084045..1084674 Francisella tularensis subsp. tularensis SCHU S4 3191071 YP_170053.1 CDS isftu1 NC_006570.2 1085435 1086219 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(1085435..1085761,1085761..1086219) Francisella tularensis subsp. tularensis SCHU S4 3190915 YP_170054.1 CDS FTT_1075 NC_006570.2 1087103 1087330 D Similar to Y4MF_RHISN (P55565) Hypothetical transcriptional regulator y4mF from Rhizobium sp. (strain NGR234) (76 aa). FASTA: opt: 205 Z-score: 292.4 E(): 2.1e-08 Smith-Waterman score: 205; 47.059identity in 68 aa overlap. ORF ftt1075; transcriptional regulator 1087103..1087330 Francisella tularensis subsp. tularensis SCHU S4 3191962 YP_170055.1 CDS hipA NC_006570.2 1087315 1088589 D Similar to Q82V03 Possible HipA protein from Nitrosomonas europaea (429 aa). FASTA: opt: 1021 Z-score: 1249.8 E(): 1e-61 Smith-Waterman score: 1021; 41.162 identity in 413 aa overlap.; transcriptional regulator 1087315..1088589 Francisella tularensis subsp. tularensis SCHU S4 3192429 YP_170058.1 CDS FTT_1079c NC_006570.2 1089907 1090167 R ORF ftt1079c; hypothetical protein complement(1089907..1090167) Francisella tularensis subsp. tularensis SCHU S4 3191961 YP_170059.1 CDS FTT_1080c NC_006570.2 1090184 1091062 R ORF ftt1080c; hypothetical protein complement(1090184..1091062) Francisella tularensis subsp. tularensis SCHU S4 3191959 YP_170060.1 CDS FTT_1081c NC_006570.2 1091062 1091865 R ORF ftt1081c; hypothetical protein complement(1091062..1091865) Francisella tularensis subsp. tularensis SCHU S4 3191958 YP_170061.1 CDS FTT_1082 NC_006570.2 1092091 1092513 D ORF ftt1082; hypothetical protein 1092091..1092513 Francisella tularensis subsp. tularensis SCHU S4 3191956 YP_170062.1 CDS FTT_1083c NC_006570.2 1092529 1092786 R ORF ftt1083c; hypothetical protein complement(1092529..1092786) Francisella tularensis subsp. tularensis SCHU S4 3191955 YP_170063.1 CDS rdgC NC_006570.2 1092791 1093651 R Required for efficient pilin antigenic variation; recombination associated protein complement(1092791..1093651) Francisella tularensis subsp. tularensis SCHU S4 3191644 YP_170064.1 CDS FTT_1085 NC_006570.2 1093833 1094831 D Similar to YM90_SHEON (Q8EES8) Hypothetical UPF0176 protein SO229 from Shewanella oneidensis (333 aa). FASTA: initn: 1561 init1: 1472 opt: 1479 Z-score: 1729.8 E(): 1.9e-88 Smith-Waterman score: 1479; 63.636identity in 330 aa overlap. ORF ftt1085; hypothetical protein 1093833..1094831 Francisella tularensis subsp. tularensis SCHU S4 3191952 YP_170065.1 CDS FTT_1086c NC_006570.2 1094962 1095792 R Similar to Q8EFU7 Conserved hypothetical protein from Shewanella oneidensis (279 aa). FASTA: 351 opt: 789 Z-score: 963.7 E(): 8.8e-46 Smith-Waterman score: 789; 45.290 identity in 276 aa overlap. ORF ftt1086c; hypothetical protein complement(1094962..1095792) Francisella tularensis subsp. tularensis SCHU S4 3191953 YP_170066.1 CDS rep NC_006570.2 1095848 1097863 R Similar to REP_HAEIN (P44804) ATP-dependent DNA helicase rep from Haemophilus influenzae (670 aa). FASTA: opt: 2055 Z-score: 2350.5 E(): 5e-123 Smith-Waterman score: 2055; 47.806 identity in 661 aa overlap.; ATP-dependent DNA helicase complement(1095848..1097863) Francisella tularensis subsp. tularensis SCHU S4 3191631 YP_170067.1 CDS FTT_1088c NC_006570.2 1097857 1098603 R ORF ftt1088c; hypothetical protein complement(1097857..1098603) Francisella tularensis subsp. tularensis SCHU S4 3191950 YP_170068.1 CDS FTT_1089 NC_006570.2 1099211 1099732 D Similar to Q8EW31 Hypothetical protein from Mycoplasma penetrans (194 aa). FASTA: opt: 235 Z-score: 294.7 E(): 1.6e-08 Smith-Waterman score: 235; 31.515 identity in 165 aa overlap. ORF ftt1089; isochorismatase hydrolase family protein 1099211..1099732 Francisella tularensis subsp. tularensis SCHU S4 3191951 YP_170069.1 CDS FTT_1090 NC_006570.2 1099729 1100406 D Similar to Q8A546 membrane transporter involved in nicotinamide mononucleotide transport from Bacteroides thetaiotaomicron (188 aa). FASTA: opt: 285 Z-score: 336.8 E(): 7.2e-11 Smith-Waterman score: 302; 29.592 identity in 196 aa overlap. ORF ftt1090; hypothetical protein 1099729..1100406 Francisella tularensis subsp. tularensis SCHU S4 3191265 YP_170070.1 CDS FTT_1091 NC_006570.2 1100419 1100907 D Similar to Q9AD62 hydrolase (193 aa). FASTA: opt: 251 Z-score: 311.7 E(): 1.8e-09 Smith-Waterman score: 251; 32.075identity in 159 aa overlap. ORF ftt1091; isochorismatase hydrolase family protein 1100419..1100907 Francisella tularensis subsp. tularensis SCHU S4 3191070 YP_170072.1 CDS talA NC_006570.2 1101545 1102510 R Similar to Q8EBH2 Transaldolase from Shewanella oneidensis (318 aa). FASTA: opt: 1030 Z-score: 1249.3 E(): 1.1e-61 Smith-Waterman score: 1030; 50.938 identity in 320 aa overlap.; transaldolase B complement(1101545..1102510) Francisella tularensis subsp. tularensis SCHU S4 3190749 YP_170073.1 CDS FTT_1094c NC_006570.2 1102644 1104059 R Similar to Q83DX0 Cytosol aminopeptidase family protein from Coxiella burnetii (458 aa). FASTA: opt: 1273 Z-score: 1536.1 E(): 1.1e-77 Smith-Waterman score: 1273; 41.830 identity in 459 aa overlap. ORF ftt1094c; cytosol aminopeptidase complement(1102644..1104059) Francisella tularensis subsp. tularensis SCHU S4 3191261 YP_170074.1 CDS FTT_1095c NC_006570.2 1104026 1105408 R Similar to Q88DA4 Outer membrane efflux protein from Pseudomonas putida (strain KT2440) (477 aa). FASTA: opt: 405 Z-score: 484.1 E(): 4.5e-19 Smith-Waterman score: 405; 22.907 identity in 454 aa overlap. Not a membrane protein. ORF ftt1095c; hypothetical protein complement(1104026..1105408) Francisella tularensis subsp. tularensis SCHU S4 3191260 YP_170075.1 CDS alaS NC_006570.2 1105416 1108013 R Catalyzes a two-step reaction, first charging an alanyl molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; alanyl-tRNA synthetase complement(1105416..1108013) Francisella tularensis subsp. tularensis SCHU S4 3192392 YP_170076.1 CDS FTT_1097 NC_006570.2 1108169 1108615 D ORF ftt1097; hypothetical protein 1108169..1108615 Francisella tularensis subsp. tularensis SCHU S4 3191259 YP_170077.1 CDS FTT_1099c NC_006570.2 1110704 1111039 R ORF ftt1099c; hypothetical protein complement(1110704..1111039) Francisella tularensis subsp. tularensis SCHU S4 3191257 YP_170078.1 CDS FTT_1100 NC_006570.2 1111142 1111477 D Similar to Q83DY7 Iojap-related protein from Coxiella burnetii (116 aa). FASTA: opt: 326 Z-score: 425.2 E(): 8.6e-16 Smith-Waterman score: 326; 41.509 identity in 106 aa overlap. ORF ftt1100; hypothetical protein 1111142..1111477 Francisella tularensis subsp. tularensis SCHU S4 3191255 YP_170079.1 CDS FTT_1103 NC_006570.2 1112962 1114059 D Similar to Q8Z9P6 exported protein from Salmonella typhi (281 aa). FASTA: opt: 392 Z-score: 435.6 E(): 2.3e-16 Smith-Waterman score: 392; 33.491 identity in 212 aa overlap. ORF ftt1103; lipoprotein 1112962..1114059 Francisella tularensis subsp. tularensis SCHU S4 3191250 YP_170080.1 CDS msrA1 NC_006570.2 1115543 1116397 R this fusion consists of methionine sulfoxide B reductase at the N-terminus and A at the C-terminus; A and B are stereospecific enzymes that recognize the damaged produces of oxidative stress, S and R epimers of methionine sulfoxide, respectively; a fusion protein of these enzymes with thioredoxin provides protection against oxidative stress in Neisseria gonorrhoeae; bifunctional methionine sulfoxide reductase B/A protein complement(1115543..1116397) Francisella tularensis subsp. tularensis SCHU S4 3191668 YP_170081.1 CDS tspO NC_006570.2 1116526 1117002 D Similar to Q8TID9 Benzodiazepine receptor TspO from Methanosarcina acetivorans (160 aa). FASTA: opt: 417 Z-score: 464.9 E(): 5.3e-18 Smith-Waterman score: 417; 40.994 identity in 161 aa overlap.; tryptophan-rich sensory protein 1116526..1117002 Francisella tularensis subsp. tularensis SCHU S4 3191010 YP_170082.1 CDS FTT_1108 NC_006570.2 1119088 1119840 D Similar to Q891T6 23S rRNA methyltransferase (EC 2.1.1.-) from Clostridium tetani (260 aa). FASTA: opt: 442 Z-score: 545.7 E(): 1.7e-22 Smith-Waterman score: 442; 34.091 identity in 264 aa overlap. ORF ftt1108; rRNA methyltransferase 1119088..1119840 Francisella tularensis subsp. tularensis SCHU S4 3191249 YP_170083.1 CDS FTT_1109 NC_006570.2 1119934 1120902 D Similar to Q8U768 Choloylglycine hydrolase family protein from Agrobacterium tumefaciens (strain C58 / ATCC 33970) (355 aa). FASTA: opt: 362 Z-score: 436.7 E(): 2e-16 Smith-Waterman score: 424; 28.956 identity in 297 aa overlap. ORF ftt1109; choloylglycine hydrolase family protein 1119934..1120902 Francisella tularensis subsp. tularensis SCHU S4 3191246 YP_170084.1 CDS FTT_1110 NC_006570.2 1121067 1121420 D ORF ftt1110; hypothetical protein 1121067..1121420 Francisella tularensis subsp. tularensis SCHU S4 3191247 YP_170085.1 CDS isftu2 NC_006570.2 1121516 1122259 D ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 transposase from Francisella tularensis (247 aa).; transposase 1121516..1122259 Francisella tularensis subsp. tularensis SCHU S4 3190832 YP_170086.1 CDS rpoH NC_006570.2 1122307 1123185 R binds with the catalytic core of RNA polymerase to produce the holoenzyme; this sigma factor is responsible for the expression of heat shock promoters; RNA polymerase factor sigma-32 complement(1122307..1123185) Francisella tularensis subsp. tularensis SCHU S4 3191978 YP_170087.1 CDS FTT_1113c NC_006570.2 1123209 1123739 R ORF ftt1113c; hypothetical protein complement(1123209..1123739) Francisella tularensis subsp. tularensis SCHU S4 3191244 YP_170088.1 CDS secF NC_006570.2 1123771 1124715 R forms a complex with SecD and YajC; SecDFyajC stimulates the proton motive force-driven protein translocation; seems to modulate the cycling of SecA by stabilizing its membrane-inserted state and appears to be required for the release of mature proteins from the extracytoplasmic side of the membrane; in some organisms, such as Bacillus subtilis, SecD is fused to SecF; preprotein translocase subunit SecF complement(1123771..1124715) Francisella tularensis subsp. tularensis SCHU S4 3191076 YP_170089.1 CDS secD NC_006570.2 1124734 1126671 R part of the preprotein secretory system; when complexed with proteins SecF and YajC, SecDFyajC stimulates the proton motive force-driven protein translocation, and appears to be required for the release of mature proteins from the extracytoplasmic side of the membrane; preprotein translocase subunit SecD complement(1124734..1126671) Francisella tularensis subsp. tularensis SCHU S4 3191719 YP_170090.1 CDS yajC NC_006570.2 1126728 1127084 R Similar to Q8XFR4 (Q8XFR4) membrane protein (Preprotein translocase IISP family, membrane subunit) from Salmonella typhi (110 aa). FASTA: opt: 347 Z-score: 458.8 E(): 1.2e-17 Smith-Waterman score: 347; 47.664 identity in 107 aa overlap.; preprotein translocase family protein complement(1126728..1127084) Francisella tularensis subsp. tularensis SCHU S4 3192124 YP_170091.1 CDS FTT_1117c NC_006570.2 1127638 1128210 R Similar to Q9HZE2 Hypothetical protein PA3066 from Pseudomonas aeruginosa (190 aa). FASTA: opt: 359 Z-score: 477.3 E(): 1.1e-18 Smith-Waterman score: 359; 35.638 identity in 188 aa overlap. ORF ftt1117c; isochorismatase hydrolase family protein complement(1127638..1128210) Francisella tularensis subsp. tularensis SCHU S4 3191243 YP_170092.1 CDS FTT_1118c NC_006570.2 1128222 1129097 R Similar to Q9KKY7 Hypothetical protein VCA0963 from Vibrio cholerae (307 aa). FASTA: opt: 340 Z-score: 385.2 E(): 1.5e-13 Smith-Waterman score: 340; 22.603 identity in 292 aa overlap. ORF ftt1118c; hypothetical protein complement(1128222..1129097) Francisella tularensis subsp. tularensis SCHU S4 3191233 YP_170093.1 CDS FTT_1119 NC_006570.2 1129202 1130077 D Similar to Q8D460 Transcriptional regulator from Vibrio vulnificus (296 aa). FASTA: opt: 459 Z-score: 575.9 E(): 3.5e-24 Smith-Waterman score: 459; 29.197 identity in 274 aa overlap. ORF ftt1119; LysR family transcriptional regulator 1129202..1130077 Francisella tularensis subsp. tularensis SCHU S4 3191673 YP_170094.1 CDS tgt NC_006570.2 1130084 1131187 R Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr); queuine tRNA-ribosyltransferase complement(1130084..1131187) Francisella tularensis subsp. tularensis SCHU S4 3190761 YP_170095.1 CDS FTT_1122c NC_006570.2 1132161 1132631 R ORF ftt1122c; lipoprotein complement(1132161..1132631) Francisella tularensis subsp. tularensis SCHU S4 3191672 YP_170096.1 CDS ygiH NC_006570.2 1133213 1133827 D Similar to Y647_YERPE (Q8ZI67) Hypothetical UPF0078 protein YPO0647/Y3532 (216 aa). FASTA: opt: 604 Z-score: 713.6 E(): 7.4e-32 Smith-Waterman score: 604; 48.500 identity in 200 aa overlap.; hypothetical protein 1133213..1133827 Francisella tularensis subsp. tularensis SCHU S4 3191266 YP_170097.1 CDS metN NC_006570.2 1133790 1134866 D Similar to AAP15732 ATP-binding component of a transporter from Shigella flexneri (343 aa). FASTA: opt: 1070 Z-score: 1232.1 E(): 9.7e-61 Smith-Waterman score: 1070; 46.221 identity in 344 aa overlap.; D-methionine ABC transporter ATP-binding protein 1133790..1134866 Francisella tularensis subsp. tularensis SCHU S4 3190821 YP_170098.1 CDS metIQ NC_006570.2 1134844 1136316 D Similar to METQ_SALTI (Q8Z992) D-methionine-binding lipoprotein metQ [Precursor] from Salmonella typhi (271 aa). FASTA: opt: 815 Z-score: 926.6 E(): 1e-43 Smith-Waterman score: 815; 51.020identity in 245 aa overlap. The FASTA-hits do not include the first 220 aa. Probably two genes, metI and metQ, in other organisms.; D-methionine ABC transporter permease/substrate-binding protein 1134844..1136316 Francisella tularensis subsp. tularensis SCHU S4 3190931 YP_170099.1 CDS FTT_1126 NC_006570.2 1136387 1137598 D Similar to Q83BG9 Tryptophan/tyrosine permease family protein from Coxiella burnetii (426 aa). FASTA: opt: 560 Z-score: 604.8 E(): 8.5e-26 Smith-Waterman score: 560; 27.750identity in 400 aa overlap. ORF ftt1126; aromatic amino acid HAAP transporter 1136387..1137598 Francisella tularensis subsp. tularensis SCHU S4 3191671 YP_170100.1 CDS FTT_1127 NC_006570.2 1137664 1138422 D Similar to Q83F83 Rhodanese domain protein from Coxiella burnetii (124 aa). FASTA: opt: 447 Z-score: 559.4 E(): 2.9e-23 Smith-Waterman score: 447; 51.240 identity in 121 aa overlap. ORF ftt1127; rhodanese-like family protein 1137664..1138422 Francisella tularensis subsp. tularensis SCHU S4 3192171 YP_170101.1 CDS ispF NC_006570.2 1138435 1138914 D catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase 1138435..1138914 Francisella tularensis subsp. tularensis SCHU S4 3190836 YP_170102.1 CDS FTT_1129c NC_006570.2 1138919 1140604 R Similar to CPHA_ANAVA (O86109) Cyanophycin synthetase from Anabaena variabilis (901 aa). FASTA: opt: 2470 Z-score: 2850.7 E(): 6.8e-151 Smith-Waterman score: 2506; 46.787 identity in 887 aa overlap. Probable paralog of cphA since the protein sequences show considerable divergence and gene length differ. ORF ftt1129c; hypothetical protein complement(1138919..1140604) Francisella tularensis subsp. tularensis SCHU S4 3191730 YP_170103.1 CDS cphA NC_006570.2 1140630 1143452 R catalyze the formation of cyanophycin which may act to store excess nitrogen; cyanophycin synthetase complement(1140630..1143452) Francisella tularensis subsp. tularensis SCHU S4 3191888 YP_008492913.1 CDS FTT_1131 NC_006570.2 1143576 1144319 D molybdopterin-binding protein 1143576..1144319 Francisella tularensis subsp. tularensis SCHU S4 3191441 YP_170104.1 CDS glpQ NC_006570.2 1144308 1145060 R Similar to Q83DQ4 Glycerophosphoryl diester phosphodiesterase, (250 aa). FASTA: opt: 306 Z-score: 366.0 E(): 1.7e-12 Smith-Waterman score: 306; 28.916identity in 249 aa overlap.; glycerophosphoryl diester phosphodiesterase complement(1144308..1145060) Francisella tularensis subsp. tularensis SCHU S4 3190955 YP_170105.1 CDS pilB NC_006570.2 1145219 1146997 D Similar to O68431 Pilus assembly protein PilB from Pseudomonas syringae (pv. tomato) (575 aa). FASTA: opt: 1381 Z-score: 1521.6 E(): 7.3e-77 Smith-Waterman score: 1592; 44.597 identity in 583 aa overlap. PilB is required for pili assembly. Homologous to pilT but which has the opposite effect. Homologs exists in other systems.; Type IV pili ABC transporter ATP-binding protein 1145219..1146997 Francisella tularensis subsp. tularensis SCHU S4 3191922 YP_170106.1 CDS pilC NC_006570.2 1147012 1148241 D Similar to Q888U1 Type IV pilus biogenesis protein PilC from Pseudomonas syringae (pv. tomato) (405 aa). FASTA: opt: 1017 Z-score: 1153.2 E(): 2.4e-56 Smith-Waterman score: 1017; 39.055identity in 402 aa overlap. Termed pilF in N. gonorrhoeae.; Type IV pili polytopic inner membrane protein 1147012..1148241 Francisella tularensis subsp. tularensis SCHU S4 3192189 YP_170107.1 CDS FTT_1136c NC_006570.2 1149485 1149820 R ORF ftt1136c; hypothetical protein complement(1149485..1149820) Francisella tularensis subsp. tularensis SCHU S4 3191658 YP_170108.1 CDS FTT_1137c NC_006570.2 1149837 1150097 R ORF ftt1137c; hypothetical protein complement(1149837..1150097) Francisella tularensis subsp. tularensis SCHU S4 3191825 YP_170109.1 CDS hemH NC_006570.2 1150231 1151232 D Similar to HEMZ_RHIME (Q92M52) Ferrochelatase (EC 4.99.1.1) from Rhizobium meliloti (Sinorhizobium meliloti) (339 aa). FASTA: opt: 1069 Z-score: 1243.4 E(): 2.3e-61 Smith-Waterman score: 1069; 48.160 identity in 326 aa overlap.; ferrochelatase 1150231..1151232 Francisella tularensis subsp. tularensis SCHU S4 3192420 YP_170110.1 CDS FTT_1140 NC_006570.2 1153449 1153646 D ORF ftt1140; hypothetical protein 1153449..1153646 Francisella tularensis subsp. tularensis SCHU S4 3191969 YP_170111.1 CDS isftu1 NC_006570.2 1153900 1154684 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(1153900..1154358,1154358..1154684)) Francisella tularensis subsp. tularensis SCHU S4 3191798 YP_170112.1 CDS FTT_1143 NC_006570.2 1155021 1155266 D ORF ftt1143; hypothetical protein 1155021..1155266 Francisella tularensis subsp. tularensis SCHU S4 3191981 YP_170113.1 CDS chaB NC_006570.2 1156322 1156528 D Similar to Q8XGJ2 Cation transport regulator ChaB from Salmonella typhi (76 aa). FASTA: opt: 267 Z-score: 394.9 E(): 4.2e-14 Smith-Waterman score: 267; 54.412 identity in 68 aa overlap.; cation transport regulator 1156322..1156528 Francisella tularensis subsp. tularensis SCHU S4 3192345 YP_170114.1 CDS dfp NC_006570.2 1157636 1158811 R Similar to DFP_HAEIN (P44953) DNA/pantothenate metabolism flavoprotein homolog from Haemophilus influenzae (400 aa). FASTA: opt: 1012 Z-score: 1206.6 E(): 2.6e-59 Smith-Waterman score: 1012; 43.577identity in 397 aa overlap.; 4'-phosphopantothenoylcysteine decarboxylase complement(1157636..1158811) Francisella tularensis subsp. tularensis SCHU S4 3191519 YP_170115.1 CDS yjhB NC_006570.2 1158824 1160008 R Similar to YJHB_ECOLI (P39352) Hypothetical metabolite transport protein yjhB from Escherichia coli (405 aa). FASTA: opt: 383 Z-score: 393.4 E(): 5.1e-14 Smith-Waterman score: 383; 26.425 identity in 386 aa overlap.; major facilitator transporter complement(1158824..1160008) Francisella tularensis subsp. tularensis SCHU S4 3192394 YP_170116.1 CDS FTT_1149c NC_006570.2 1160084 1161592 R Similar to Q8XT33 Probable amino-acid permease transmembrane from Ralstonia solanacearum (Pseudomonas solanacearum) (543 aa). FASTA: opt: 960 Z-score: 1077.6 E(): 3.9e-52 Smith-Waterman score: 969; 35.730 identity in 445 aa overlap. ORF ftt1149c; amino acid transporter family protein complement(1160084..1161592) Francisella tularensis subsp. tularensis SCHU S4 3191748 YP_170117.1 CDS putA NC_006570.2 1161752 1165825 R proline utilization protein A; multifunctional protein that functions in proline catabolism in the first two enzymatic steps resulting in the conversion of proline to glutamate; in Escherichia coli this protein self regulates transcription via a DNA-binding domain at the N-terminus but the protein from Pseudomonas does not have this domain; bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase complement(1161752..1165825) Francisella tularensis subsp. tularensis SCHU S4 3191660 YP_170118.1 CDS FTT_1151c NC_006570.2 1165833 1166120 R Similar to Q87LM9 Conserved hypothetical protein from Vibrio parahaemolyticus (86 aa). FASTA: opt: 176 Z-score: 242.1 E(): 1.4e-05 Smith-Waterman score: 176; 32.857 identity in 70 aa overlap. ORF ftt1151c; hypothetical protein complement(1165833..1166120) Francisella tularensis subsp. tularensis SCHU S4 3191681 YP_170119.1 CDS FTT_1152 NC_006570.2 1166334 1166717 D ORF ftt1152; hypothetical protein 1166334..1166717 Francisella tularensis subsp. tularensis SCHU S4 3192179 YP_170120.1 CDS FTT_1153c NC_006570.2 1166784 1167008 R ORF ftt1153c; hypothetical protein complement(1166784..1167008) Francisella tularensis subsp. tularensis SCHU S4 3192176 YP_170121.1 CDS aroB NC_006570.2 1167066 1168145 R catalyzes the formation of 3-dehydroquinate from 3-deoxy-arabino-heptulonate 7-phosphate; functions in aromatic amino acid biosynthesis; 3-dehydroquinate synthase complement(1167066..1168145) Francisella tularensis subsp. tularensis SCHU S4 3190946 YP_170122.1 CDS aroK NC_006570.2 1168142 1168672 R type I enzyme similar to type II but differentially regulated; major shikimate kinase in fully repressed cells; catalyzes the formation of shikimate 3-phosphate from shikimate in aromatic amino acid biosynthesis; shikimate kinase I complement(1168142..1168672) Francisella tularensis subsp. tularensis SCHU S4 3192541 YP_170123.1 CDS pilQ NC_006570.2 1168798 1170582 R Similar to Q8EK21 Type IV pilus biogenesis protein PilQ from Shewanella oneidensis (684 aa). FASTA: opt: 1014 Z-score: 1145.2 E(): 6.8e-56 Smith-Waterman score: 1014; 31.658 identity in 597 aa overlap. The nearest gene neighbours show similarity to pilN, pilO and pilP. Also required for type II protein secretion.; Type IV pilin multimeric outer membrane protein complement(1168798..1170582) Francisella tularensis subsp. tularensis SCHU S4 3191514 YP_170124.1 CDS FTT_1157c NC_006570.2 1170595 1171185 R Similar to Q8DCM3 Tfp pilus assembly protein PilP from Vibrio vulnificus (170 aa). FASTA: opt: 176 Z-score: 222.1 E(): 0.00018 Smith-Waterman score: 176; 29.921 identity in 127 aa overlap. Weak similarity to the lipoprotein pilP, which appears to be required for stabilisation of pilQ multimer formation. The nearest gene neighbours show similarity to pilN, pilO and pilQ, which indicates that this protein could be pilP ORF ftt1157c; Type IV pili lipoprotein complement(1170595..1171185) Francisella tularensis subsp. tularensis SCHU S4 3191399 YP_170125.1 CDS FTT_1158c NC_006570.2 1171191 1171787 R Similar to Q8PH76 Fimbrial assembly membrane protein from Xanthomonas axonopodis (pv. citri) (221 aa). FASTA: opt: 144 Z-score: 177.4 E(): 0.055 Smith-Waterman score: 144; 25.946 identity in 185 aa overlap. Weak similarity to type IV pili glycosylation protein (pilO). The nearest gene neighbours show similarity to pilN, pilP and pilQ, which indicates that this protein could be pilO. PilO is required for glycosylation of Pseudomonas aeruginosa pilin. ORF ftt1158c; Type IV pili glycosylation protein complement(1171191..1171787) Francisella tularensis subsp. tularensis SCHU S4 3191034 YP_170126.1 CDS FTT_1159c NC_006570.2 1171781 1172344 R Similar to Q52540 Membrane protein PilN from Pseudomonas syringae (199 aa). FASTA: opt: 143 Z-score: 182.6 E(): 0.028 Smith-Waterman score: 143; 23.704 identity in 135 aa overlap. The nearest gene neighbours show similarity to pilO, pilP and pilQ, which indicates that this protein could be pilN. PilN is also required for type II protein secretion. ORF ftt1159c; Type IV pili associated protein complement(1171781..1172344) Francisella tularensis subsp. tularensis SCHU S4 3191614 YP_170127.1 CDS FTT_1160c NC_006570.2 1172346 1173350 R ORF ftt1160c; hypothetical protein complement(1172346..1173350) Francisella tularensis subsp. tularensis SCHU S4 3192170 YP_170128.1 CDS adk NC_006570.2 1173477 1174133 D essential enzyme that recycles AMP in active cells; converts ATP and AMP to two molecules of ADP; adenylate kinase 1173477..1174133 Francisella tularensis subsp. tularensis SCHU S4 3192396 YP_170129.1 CDS FTT_1163c NC_006570.2 1177474 1177890 R ORF ftt1163c; hypothetical protein complement(1177474..1177890) Francisella tularensis subsp. tularensis SCHU S4 3192471 YP_170130.1 CDS era NC_006570.2 1177905 1178798 R Era; Escherichia coli Ras-like protein; Bex; Bacillus Era-complementing segment; essential protein in Escherichia coli that is involved in many cellular processes; GTPase; binds the cell membrane through apparent C-terminal domain; mutants are arrested during the cell cycle; Streptococcus pneumoniae Era binds to RNA and Escherichia coli Era binds 16S rRNA and 30S ribosome; GTP-binding protein Era complement(1177905..1178798) Francisella tularensis subsp. tularensis SCHU S4 3191858 YP_170131.1 CDS aspC2 NC_006570.2 1178912 1180105 R Similar to Q83E19 Aspartate aminotransferase from Coxiella burnetii (394 aa). FASTA: opt: 1067 Z-score: 1292.7 E(): 4.1e-64 Smith-Waterman score: 1067; 41.645 identity in 389 aa overlap.; aspartate aminotransferase complement(1178912..1180105) Francisella tularensis subsp. tularensis SCHU S4 3192538 YP_170132.1 CDS FTT_1166c NC_006570.2 1180115 1180921 R Similar to Q8D5X6 Predicted glutamine amidotransferase from Vibrio vulnificus (253 aa). FASTA: opt: 654 Z-score: 813.9 E(): 1.9e-37 Smith-Waterman score: 654; 42.466identity in 219 aa overlap. ORF ftt1166c; glutamine amidotransferase complement(1180115..1180921) Francisella tularensis subsp. tularensis SCHU S4 3192326 YP_170133.1 CDS FTT_1167c NC_006570.2 1180925 1181563 R Similar to Q8EE04 (Q8EE04) Conserved hypothetical protein from Shewanella oneidensis (236 aa). FASTA: opt: 349 Z-score: 425.3 E(): 8.5e-16 Smith-Waterman score: 359; 32.273 identity in 220 aa overlap. ORF ftt1167c; glycoprotease family protein complement(1180925..1181563) Francisella tularensis subsp. tularensis SCHU S4 3192328 YP_170134.1 CDS nagA NC_006570.2 1181567 1182700 R Similar to Q99VS2 NagA protein (Probable N-acetylglucosamine-6-phosphate deacetylase) from Staphylococcus aureus (393 aa). FASTA: opt: 1006 Z-score: 1213.0 E(): 1.1e-59 Smith-Waterman score: 1006; 43.523 identity in 386 aa overlap.; N-acetylglucosamine-6-phosphate deacetylase complement(1181567..1182700) Francisella tularensis subsp. tularensis SCHU S4 3192442 YP_170135.1 CDS FTT_1169 NC_006570.2 1182763 1183635 D Similar to Q8EE00 GTP-binding protein (313 aa). FASTA: opt: 793 Z-score: 926.5 E(): 1e-43 Smith-Waterman score: 793; 44.218 identity in 294 aa overlap. ORF ftt1169; GTP-binding protein 1182763..1183635 Francisella tularensis subsp. tularensis SCHU S4 3190858 YP_170136.1 CDS FTT_1170 NC_006570.2 1183699 1185447 D ORF ftt1170; lipoprotein 1183699..1185447 Francisella tularensis subsp. tularensis SCHU S4 3190838 YP_170137.1 CDS hsdM NC_006570.2 1185437 1186903 R Similar to Q8RN13 HsdM from Campylobacter jejuni (494 aa). FASTA: opt: 2001 Z-score: 2374.3 E(): 2.3e-124 Smith-Waterman score: 2001; 59.719 identity in 499 aa overlap.; DNA-methyltransferase, type I restriction-modification enzyme subunit M complement(1185437..1186903) Francisella tularensis subsp. tularensis SCHU S4 3190753 YP_170138.1 CDS csp NC_006570.2 1186890 1187321 R Similar to Q9HSS3 Cold shock protein from Halobacterium sp. (strain NRC-1 / ATCC 700922 / JCM 11081) (74 aa). FASTA: opt: 170 Z-score: 221.1 E(): 0.0002 Smith-Waterman score: 170; 40.323identity in 62 aa overlap. CDS is longer than FASTA matches. Homology is to first 70 aa of protein only and no matches found for 2nd half of CDS; DNA-binding cold shock protein complement(1186890..1187321) Francisella tularensis subsp. tularensis SCHU S4 3191897 YP_170139.1 CDS FTT_1174c NC_006570.2 1188833 1189042 R ORF ftt1174c; hypothetical protein complement(1188833..1189042) Francisella tularensis subsp. tularensis SCHU S4 3192318 YP_170140.1 CDS FTT_1175c NC_006570.2 1189242 1189880 R ORF ftt1175c; hypothetical protein complement(1189242..1189880) Francisella tularensis subsp. tularensis SCHU S4 3192329 YP_170143.1 CDS bipA NC_006570.2 1194339 1196156 D Similar to Q9KNJ4 GTP-binding protein TypA from Vibrio cholerae (609 aa). FASTA: opt: 2972 Z-score: 3319.9 E(): 5e-177 Smith-Waterman score: 2972; 73.045identity in 601 aa overlap.; GTP binding translational elongation factor Tu and G family protein 1194339..1196156 Francisella tularensis subsp. tularensis SCHU S4 3190966 YP_170144.1 CDS ggt NC_006570.2 1197099 1198904 R Similar to Q8EFL9 Gamma-glutamyltranspeptidase from Shewanella oneidensis (579 aa). FASTA: opt: 2002 Z-score: 2308.7 E(): 1.1e-120 Smith-Waterman score: 2002; 54.545 identity in 572 aa overlap.; gamma-glutamyltranspeptidase complement(1197099..1198904) Francisella tularensis subsp. tularensis SCHU S4 3192241 YP_170145.1 CDS FTT_1183c NC_006570.2 1199956 1201161 R Similar to YFCK_ECO57 (Q8XCQ7) Hypothetical protein yfcK from Escherichia coli (668 aa). FASTA: opt: 495 Z-score: 572.0 E(): 5.7e-24 Smith-Waterman score: 495; 28.087 identity in 413 aa overlap. ORF ftt1183c; lipoprotein complement(1199956..1201161) Francisella tularensis subsp. tularensis SCHU S4 3191007 YP_170146.1 CDS FTT_1184c NC_006570.2 1201203 1201508 R Similar to Y369_VIBVU (Q8DF55) Hypothetical protein VV10369 from Vibrio vulnificus (117 aa). FASTA: opt: 238 Z-score: 320.2 E(): 6.1e-10 Smith-Waterman score: 238; 41.304 identity in 92 aa overlap. ORF ftt1184c; hypothetical protein complement(1201203..1201508) Francisella tularensis subsp. tularensis SCHU S4 3191004 YP_170147.1 CDS FTT_1185c NC_006570.2 1201508 1201939 R Similar to Q8ZH13 Hypothetical protein YPO1102 from Yersinia pestis (144 aa). FASTA: opt: 463 Z-score: 623.4 E(): 7.9e-27 Smith-Waterman score: 463; 43.662 39dentity in 142 aa overlap. ORF ftt1185c; hypothetical protein complement(1201508..1201939) Francisella tularensis subsp. tularensis SCHU S4 3191001 YP_170148.1 CDS smpB NC_006570.2 1202014 1202487 D binds to ssrA RNA (tmRNA) and is required for its successful binding to ribosomes; also appears to function in the trans-translation step by promoting accommodation of tmRNA into the ribosomal A site; SmpB protects the tmRNA from RNase R degradation in Caulobacter crescentus; both the tmRNA and SmpB are regulated in cell cycle-dependent manner; functions in release of stalled ribosomes from damaged mRNAs and targeting proteins for degradation; SsrA-binding protein 1202014..1202487 Francisella tularensis subsp. tularensis SCHU S4 3190711 YP_170149.1 CDS FTT_1187 NC_006570.2 1202488 1203243 D TtcA; YdaO; catalyzes the thiolation of cytosine 32 in specific tRNAs; C32 tRNA thiolase 1202488..1203243 Francisella tularensis subsp. tularensis SCHU S4 3190998 YP_170150.1 CDS FTT_1188 NC_006570.2 1203515 1204150 D ORF ftt1188; hypothetical protein 1203515..1204150 Francisella tularensis subsp. tularensis SCHU S4 3190993 YP_170151.1 CDS xseA NC_006570.2 1205946 1207331 R bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides; exodeoxyribonuclease VII large subunit complement(1205946..1207331) Francisella tularensis subsp. tularensis SCHU S4 3192120 YP_170152.1 CDS FTT_1191 NC_006570.2 1207484 1208644 D Similar to Q8RFU4 N-acyl-L-amino acid amidohydrolase from Fusobacterium nucleatum (subsp. nucleatum) (393 aa). FASTA: opt: 945 Z-score: 1170.3 E(): 2.7e-57 Smith-Waterman score: 945; 38.259 identity in 379 aa overlap. ORF ftt1191; aminoacylase 1207484..1208644 Francisella tularensis subsp. tularensis SCHU S4 3190989 YP_170154.1 CDS FTT_1194c NC_006570.2 1210462 1211388 R Similar to Q884M8 Conserved hypothetical protein from Pseudomonas syringae (pv. tomato) (319 aa). FASTA: opt: 460 Z-score: 588.5 E(): 6.9e-25 Smith-Waterman score: 460; 30.435identity in 322 aa overlap. pseudogene (upstream sequence matches homologs). ORF ftt1194c; lipoprotein complement(1210462..1211388) Francisella tularensis subsp. tularensis SCHU S4 3192282 YP_170155.1 CDS FTT_1196c NC_006570.2 1213063 1214346 R Similar to Q9ZC87 Proline/betaine transporter (PROP7) from Rickettsia prowazekii (423 aa). FASTA: opt: 746 Z-score: 810.0 E(): 3.2e-37 Smith-Waterman score: 746; 31.450identity in 407 aa overlap. ORF ftt1196c; major facilitator transporter complement(1213063..1214346) Francisella tularensis subsp. tularensis SCHU S4 3192271 YP_170156.1 CDS murI NC_006570.2 1214431 1215228 R Similar to MURI_NEIMC (Q9RMM3) Glutamate racemase (EC 5.1.1.3) from Neisseria meningitidis (serogroup C) (270 aa). FASTA: opt: 826 Z-score: 958.8 E(): 1.6e-45 Smith-Waterman score: 826; 48.473identity in 262 aa overlap.; glutamate racemase complement(1214431..1215228) Francisella tularensis subsp. tularensis SCHU S4 3192033 YP_170157.1 CDS FTT_1198c NC_006570.2 1215228 1216058 R ORF ftt1198c; hypothetical protein complement(1215228..1216058) Francisella tularensis subsp. tularensis SCHU S4 3192270 YP_170158.1 CDS uvrB NC_006570.2 1216062 1218068 R The UvrABC repair system catalyzes the recognition and processing of DNA lesions. The beta-hairpin of the Uvr-B subunit is inserted between the strands, where it probes for the presence of a lesion; excinuclease ABC subunit B complement(1216062..1218068) Francisella tularensis subsp. tularensis SCHU S4 3191920 YP_170159.1 CDS sbcB NC_006570.2 1218070 1219518 R Similar to Q83BX4 Exodeoxyribonuclease I, from Coxiella burnetii (458 aa). FASTA: opt: 677 Z-score: 797.9 E(): 1.5e-36 Smith-Waterman score: 1190; 39.875 identity in 479 aa overlap.; exodeoxyribonuclease I complement(1218070..1219518) Francisella tularensis subsp. tularensis SCHU S4 3191444 YP_170160.1 CDS FTT_1201c NC_006570.2 1219652 1220374 R Similar to Q9KKP5 Oxidoreductase,short-chain dehydrogenase/reductase family from Vibrio cholerae (239 aa). FASTA: opt: 1097 Z-score: 1344.5 E(): 5.4e-67 Smith-Waterman score: 1097; 72.458identity in 236 aa overlap. ORF ftt1201c; short-chain dehydrogenase family oxidoreductase complement(1219652..1220374) Francisella tularensis subsp. tularensis SCHU S4 3192265 YP_170161.1 CDS FTT_1202 NC_006570.2 1220490 1221389 D Similar to Q9KKP4 Transcriptional regulator, LysR family from Vibrio cholerae (301 aa). FASTA: opt: 661 Z-score: 794.3 E(): 2.4e-36 Smith-Waterman score: 661; 36.120identity in 299 aa overlap. ORF ftt1202; LysR family transcriptional regulator 1220490..1221389 Francisella tularensis subsp. tularensis SCHU S4 3192261 YP_170162.1 CDS FTT_1203c NC_006570.2 1221400 1222305 R ORF ftt1203c; hypothetical protein complement(1221400..1222305) Francisella tularensis subsp. tularensis SCHU S4 3192257 YP_170163.1 CDS FTT_1204c NC_006570.2 1222292 1223374 R ORF ftt1204c; hypothetical protein complement(1222292..1223374) Francisella tularensis subsp. tularensis SCHU S4 3192253 YP_170164.1 CDS gidA NC_006570.2 1223517 1225400 D GidA; glucose-inhibited cell division protein A; involved in the 5-carboxymethylaminomethyl modification (mnm(5)s(2)U) of the wobble uridine base in some tRNAs; tRNA uridine 5-carboxymethylaminomethyl modification protein GidA 1223517..1225400 Francisella tularensis subsp. tularensis SCHU S4 3192517 YP_170165.1 CDS FTT_1206 NC_006570.2 1225412 1225804 D ORF ftt1206; hypothetical protein 1225412..1225804 Francisella tularensis subsp. tularensis SCHU S4 3192252 YP_170166.1 CDS FTT_1207 NC_006570.2 1225818 1227188 D ORF ftt1207; hypothetical protein 1225818..1227188 Francisella tularensis subsp. tularensis SCHU S4 3192249 YP_170167.1 CDS rpiA NC_006570.2 1227190 1227864 D Catalyzes D-ribose 5-phosphate --> D-ribulose 5-phosphate in the nonoxidative branch of the pentose phosphate pathway; ribose-5-phosphate isomerase A 1227190..1227864 Francisella tularensis subsp. tularensis SCHU S4 3192024 YP_170168.1 CDS isftu1 NC_006570.2 1229938 1230722 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(1229938..1230396,1230396..1230722)) Francisella tularensis subsp. tularensis SCHU S4 3190805 YP_170169.1 CDS FTT_1211c NC_006570.2 1230784 1231176 R CDS is disrupted by the insertion of an ISFtu1 element occurring very near the C-terminal end. It is not clear whether this insertion affects the function of the protein ORF ftt1211c; hypothetical protein complement(1230784..1231176) Francisella tularensis subsp. tularensis SCHU S4 3192240 YP_170170.1 CDS gloA NC_006570.2 1231176 1231559 R Similar to LGUL_VIBPA (P46235) Probable lactoylglutathione lyase from Vibrio parahaemolyticus (138 aa). FASTA: opt: 549 Z-score: 759.1 E(): 2.2e-34 Smith-Waterman score: 549; 64.516 identity in 124 aa overlap.; lactoylglutathione lyase complement(1231176..1231559) Francisella tularensis subsp. tularensis SCHU S4 3192332 YP_170171.1 CDS FTT_1213 NC_006570.2 1231675 1232388 D Similar to YPDP_BACSU (P54163) Hypothetical protein ypdP from Bacillus subtilis (229 aa). FASTA: opt: 472 Z-score: 563.3 E(): 1.7e-23 Smith-Waterman score: 472; 40.435 identity in 230 aa overlap. ORF ftt1213; hypothetical protein 1231675..1232388 Francisella tularensis subsp. tularensis SCHU S4 3192236 YP_170172.1 CDS FTT_1214c NC_006570.2 1232642 1233433 R Similar to Q8XN14 Hypothetical protein CPE0524 from Clostridium perfringens (264 aa). FASTA: opt: 486 Z-score: 584.5 E(): 1.1e-24 Smith-Waterman score: 486; 31.939 identity in 263 aa overlap. ORF ftt1214c; haloacid dehalogenase complement(1232642..1233433) Francisella tularensis subsp. tularensis SCHU S4 3192234 YP_170173.1 CDS queA NC_006570.2 1234068 1235090 R Synthesizes oQ from preQ1 in a single S-adenosylmethionine-requiring step; S-adenosylmethionine--tRNA ribosyltransferase-isomerase complement(1234068..1235090) Francisella tularensis subsp. tularensis SCHU S4 3191648 YP_170174.1 CDS FTT_1216c NC_006570.2 1235110 1235571 R ORF ftt1216c; hypothetical protein complement(1235110..1235571) Francisella tularensis subsp. tularensis SCHU S4 3192235 YP_170175.1 CDS visC NC_006570.2 1235593 1236828 R Similar to Q83F72 VisC protein from Coxiella burnetii (411 aa). FASTA: opt: 645 Z-score: 778.5 E(): 1.8e-35 Smith-Waterman score: 858; 37.192 identity in 406 aa overlap.; monooxygenase family protein complement(1235593..1236828) Francisella tularensis subsp. tularensis SCHU S4 3191940 YP_170176.1 CDS ubiH NC_006570.2 1236818 1238029 R Similar to Q87UH4 2-octaprenyl-6-methoxyphenyl hydroxylase from Pseudomonas syringae (pv. tomato) (395 aa). FASTA: opt: 654 Z-score: 770.5 E(): 5e-35 Smith-Waterman score: 654; 32.143 identity in 392 aa overlap.; 2-octaprenyl-6-methoxyphenyl hydroxylase complement(1236818..1238029) Francisella tularensis subsp. tularensis SCHU S4 3191137 YP_170177.1 CDS FTT_1219c NC_006570.2 1238038 1238574 R Similar to YGA1_PSEPK (Q88CI0) Hypothetical UPF0149 protein PP5201 from Pseudomonas putida (strain KT2440) (184 aa). FASTA: opt: 269 Z-score: 349.3 E(): 1.4e-11 Smith-Waterman score: 269; 34.637identity in 179 aa overlap. ORF ftt1219c; hypothetical protein complement(1238038..1238574) Francisella tularensis subsp. tularensis SCHU S4 3192233 YP_170178.1 CDS FTT_1220 NC_006570.2 1238975 1239523 D Similar to Q8ZM70 ligase from Salmonella typhimurium (182 aa). FASTA: opt: 317 Z-score: 411.1 E(): 5.2e-15 Smith-Waterman score: 317; 32.727 identity in 165 aa overlap. ORF ftt1220; 5-formyltetrahydrofolate cyclo-ligase 1238975..1239523 Francisella tularensis subsp. tularensis SCHU S4 3192232 YP_170179.1 CDS FTT_1221 NC_006570.2 1239526 1239792 D ORF ftt1221; hypothetical protein 1239526..1239792 Francisella tularensis subsp. tularensis SCHU S4 3192229 YP_170180.1 CDS dedA2 NC_006570.2 1239799 1240425 D Similar to Q89YU2 Conserved hypothetical protein, membrane protein from Bacteroides thetaiotaomicron (213 aa). FASTA: opt: 602 Z-score: 702.4 E(): 3.1e-31 Smith-Waterman score: 602; 42.857identity in 210 aa overlap.; DedA family protein 1239799..1240425 Francisella tularensis subsp. tularensis SCHU S4 3191509 YP_170181.1 CDS dedA1 NC_006570.2 1240481 1241128 D Similar to Q8R8Y8 Hypothetical protein DedA2 from Thermoanaerobacter tengcongensis (209 aa). FAST: opt: 674 Z-score: 828.7 E(): 2.9e-38 Smith-Waterman score: 674; 45.894 identity in 207 aa overlap.; DedA family protein 1240481..1241128 Francisella tularensis subsp. tularensis SCHU S4 3191510 YP_170182.1 CDS recO NC_006570.2 1241204 1241902 R Similar to RECO_VIBVU (Q8DC74) DNA repair protein recO from Vibrio vulnificus (243 aa). FASTA: opt: 244 Z-score: 288.3 E(): 3.6e-08 Smith-Waterman score: 244; 29.130 identity in 230 aa overlap.; DNA repair protein RecO complement(1241204..1241902) Francisella tularensis subsp. tularensis SCHU S4 3191635 YP_170183.1 CDS FTT_1225c NC_006570.2 1241886 1242215 R ORF ftt1225c; hypothetical protein complement(1241886..1242215) Francisella tularensis subsp. tularensis SCHU S4 3192227 YP_170184.1 CDS iscS NC_006570.2 1242208 1243383 R Similar to ISCS_VIBCH (Q9KTY2) Cysteine desulfurase (EC 4.4.1.-) from Vibrio cholerae (404 aa). FASTA: opt: 1104 Z-score: 1336.0 E(): 1.6e-66 Smith-Waterman score: 1104; 45.812 identity in 382 aa overlap.; cysteine desulfurase complement(1242208..1243383) Francisella tularensis subsp. tularensis SCHU S4 3191911 YP_170185.1 CDS rne NC_006570.2 1243633 1246290 D Similar to Q9KQG8 Ribonuclease E from Vibrio cholerae (1052 aa). FASTA: opt: 1928 Z-score: 1743.2 E(): 3.3e-89 Smith-Waterman score: 1943; 43.377 identity in 770 aa overlap.; ribonuclease E 1243633..1246290 Francisella tularensis subsp. tularensis SCHU S4 3191610 YP_170186.1 CDS lgt NC_006570.2 1246305 1247111 D transfers the N-acyl diglyceride moiety to the prospective N-terminal cysteine in prolipoprotein; prolipoprotein diacylglyceryl transferase 1246305..1247111 Francisella tularensis subsp. tularensis SCHU S4 3190760 YP_170187.1 CDS thyA NC_006570.2 1247096 1247920 D ThyA; catalyzes formation of dTMP and 7,8-dihydrofolate from 5,10-methylenetetrahydrofolate and dUMP; involved in deoxyribonucleotide biosynthesis; there are 2 copies in some Bacilli, one of which appears to be phage-derived; thymidylate synthase 1247096..1247920 Francisella tularensis subsp. tularensis SCHU S4 3190786 YP_170188.1 CDS serA NC_006570.2 1247923 1249167 D catalyzes the formation of 3-phosphonooxypyruvate from 3-phospho-D-glycerate in serine biosynthesis; can also reduce alpha ketoglutarate to form 2-hydroxyglutarate; D-3-phosphoglycerate dehydrogenase 1247923..1249167 Francisella tularensis subsp. tularensis SCHU S4 3191850 YP_170189.1 CDS mesJ NC_006570.2 1249168 1250364 D Similar to Q83BJ9 MesJ protein from Coxiella burnetii (449 aa). FASTA: opt: 639 Z-score: 733.1 E(): 6.1e-33 Smith-Waterman score: 726; 31.387identity in 411 aa overlap. Thought to be a cell cycle protein; PP-loop family protein 1249168..1250364 Francisella tularensis subsp. tularensis SCHU S4 3191862 YP_170190.1 CDS isftu1 NC_006570.2 1250509 1251293 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(1250509..1250835,1250835..1251293) Francisella tularensis subsp. tularensis SCHU S4 3190831 YP_170191.1 CDS yjdL NC_006570.2 1251367 1252695 R Similar to YJDL_ECOLI (P39276) Hypothetical transporter yjdL from Escherichia coli (485 aa).FASTA: opt: 1064 Z-score: 1135.9 E(): 2.2e-55 Smith-Waterman score: 1064; 38.222 identity in 450 aa overlap.This CDS is disrupted by the insertion of an ISFtu1 element occurring very near the C-terminal end. It is not clear whether this insertion affects the function of the protein; proton-dependent oligopeptide transport (POT) family protein complement(1251367..1252695) Francisella tularensis subsp. tularensis SCHU S4 3191280 YP_170192.1 CDS FTT_1234 NC_006570.2 1252946 1254091 D Similar to Q8YIA2 Choloylglycine hydrolase (EC 3.5.1.24) from Brucella melitensis (367 aa). FASTA: opt: 387 Z-score: 460.1 E(): 9.7e-18 Smith-Waterman score: 574; 34.718 identity in 337 aa overlap. ORF ftt1234; choloylglycine hydrolase family protein 1252946..1254091 Francisella tularensis subsp. tularensis SCHU S4 3192226 YP_170193.1 CDS lpcC NC_006570.2 1254092 1255156 R Similar to Q8FZH8 Lipopolysaccharide core biosynthesis mannosyltransferase LpcC from Brucella suis (354 aa). FASTA: opt: 825 Z-score: 1005.6 E(): 4e-48 Smith-Waterman score: 825; 38.352 identity in 352 aa overlap.; glycosyl transferase family protein complement(1254092..1255156) Francisella tularensis subsp. tularensis SCHU S4 3190861 YP_170194.1 CDS FTT_1236 NC_006570.2 1255326 1256414 D ORF ftt1236; hypothetical protein 1255326..1256414 Francisella tularensis subsp. tularensis SCHU S4 3192220 YP_170195.1 CDS FTT_1237 NC_006570.2 1256422 1257312 D Similar to Q8A3T1 stress protein from Bacteroides thetaiotaomicron (309 aa). FASTA: opt: 308 Z-score: 375.2 E(): 5.2e-13 Smith-Waterman score: 348; 26.599 identity in 297 aa overlap. ORF ftt1237; glycosyl transferase family protein 1256422..1257312 Francisella tularensis subsp. tularensis SCHU S4 3192224 YP_170196.1 CDS FTT_1238c NC_006570.2 1257338 1258669 R ORF ftt1238c; hypothetical protein complement(1257338..1258669) Francisella tularensis subsp. tularensis SCHU S4 3192211 YP_170197.1 CDS FTT_1239 NC_006570.2 1258877 1260148 D ORF ftt1239; hypothetical protein 1258877..1260148 Francisella tularensis subsp. tularensis SCHU S4 3192217 YP_170198.2 CDS FTT_1240c NC_006570.2 1260163 1260711 R Similar to YJGA_HAEIN (P45076) Hypothetical protein HI1151 from Haemophilus influenzae (178 aa). FASTA: opt: 359 Z-score: 390.6 E(): 7.3e-14 Smith-Waterman score: 359; 35.928 identity in 167 aa overlap. ORF ftt1240c; hypothetical protein complement(1260163..1260711) Francisella tularensis subsp. tularensis SCHU S4 3192214 YP_170199.1 CDS glyA NC_006570.2 1260815 1262068 D catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate; serine hydroxymethyltransferase 1260815..1262068 Francisella tularensis subsp. tularensis SCHU S4 3191778 YP_170200.1 CDS FTT_1242 NC_006570.2 1262350 1263612 D ORF ftt1242; hypothetical protein 1262350..1263612 Francisella tularensis subsp. tularensis SCHU S4 3192215 YP_170201.1 CDS mreA NC_006570.2 1263494 1265347 R Similar to EAA32028 Hypothetical protein from Neurospora crassa (567 aa). FASTA: opt: 643 Z-score: 759.7 E(): 2e-34 Smith-Waterman score: 793; 29.879 identity in 579 aa overlap.; FAD binding family protein complement(1263494..1265347) Francisella tularensis subsp. tularensis SCHU S4 3192019 YP_170202.1 CDS yfiO NC_006570.2 1265424 1266248 R Similar to Q8XYX9 Probable transmembrane protein from Ralstonia solanacearum (Pseudomonas solanacearum) (289 aa). FASTA: opt: 563 Z-score: 689.8 E(): 1.6e-30 Smith-Waterman score: 563; 32.558 identity in 258 aa overlap.; lipoprotein complement(1265424..1266248) Francisella tularensis subsp. tularensis SCHU S4 3192165 YP_170203.1 CDS rluD NC_006570.2 1266337 1267335 D Similar to Q87S65 Ribosomal large subunit pseudouridine from Vibrio parahaemolyticus (325 aa). FASTA: opt: 917 Z-score: 1118.2 E(): 2.2e-54 Smith-Waterman score: 917; 47.468 identity in 316 aa overlap.; ribosomal large subunit pseudouridine synthase D 1266337..1267335 Francisella tularensis subsp. tularensis SCHU S4 3191615 YP_170204.1 CDS FTT_1246 NC_006570.2 1267277 1268125 D Similar to Q8EEM6 Bax protein, from Shewanella oneidensis (255 aa). FASTA: opt: 369 Z-score: 448.5 E(): 4.3e-17 Smith-Waterman score: 369; 31.081 identity in 222 aa overlap. ORF ftt1246; hypothetical protein 1267277..1268125 Francisella tularensis subsp. tularensis SCHU S4 3192204 YP_170205.1 CDS FTT_1247 NC_006570.2 1268200 1269300 D Similar to Q9A5X9 ABC transporter,permease protein, from Caulobacter crescentus.(371 aa). FASTA: opt: 755 Z-score: 860.3 E(): 5e-40 Smith-Waterman score: 755; 36.095 identity in 338 aa overlap. ORF ftt1247; ABC transporter permease 1268200..1269300 Francisella tularensis subsp. tularensis SCHU S4 3192202 YP_170206.1 CDS FTT_1248 NC_006570.2 1269315 1270076 D Similar to Q83DK2 ABC transporter,ATP-binding protein from Coxiella burnetii (268 aa). FASTA: opt: 674 Z-score: 783.9 E(): 9e-36 Smith-Waterman score: 674; 46.188 identity in 223 aa overlap. ORF ftt1248; ABC transporter ATP-binding protein 1269315..1270076 Francisella tularensis subsp. tularensis SCHU S4 3192198 YP_170207.1 CDS FTT_1249 NC_006570.2 1270086 1270811 D Similar to Q9HZ27 Hypothetical protein PA3213 from Pseudomonas aeruginosa (312 aa). FASTA: opt: 282 Z-score: 315.9 E(): 1.1e-09 Smith-Waterman score: 282; 23.661 identity in 224 aa overlap. The archetype (Rv0169) for mce related family proteins, was isolated as being necessary for colonization of, and survival within, the macrophage MEDLINE:93377076. ORF ftt1249; cell entry (mce) family protein 1270086..1270811 Francisella tularensis subsp. tularensis SCHU S4 3192197 YP_170208.1 CDS FTT_1250 NC_006570.2 1270816 1271514 D ORF ftt1250; hypothetical protein 1270816..1271514 Francisella tularensis subsp. tularensis SCHU S4 3192195 YP_170209.1 CDS FTT_1251c NC_006570.2 1271504 1272190 R Similar to Q8EDF8 Conserved hypothetical protein from Shewanella oneidensis (234 aa). FASTA: opt: 692 Z-score: 805.5 E(): 5.6e-37 Smith-Waterman score: 692; 44.828 identity in 232 aa overlap. ORF ftt1251c; hypothetical protein complement(1271504..1272190) Francisella tularensis subsp. tularensis SCHU S4 3191080 YP_170210.1 CDS FTT_1252 NC_006570.2 1272261 1272989 D in Escherichia coli RsmE methylates the N3 position of the U1498 base in 16S rRNA; cells lacking this function can grow, but are outcompeted by wild-type; SAM-dependent m(3)U1498 methyltransferase; 16S ribosomal RNA methyltransferase RsmE 1272261..1272989 Francisella tularensis subsp. tularensis SCHU S4 3192555 YP_170211.1 CDS FTT_1253 NC_006570.2 1273219 1274706 D Similar to Q9KTB5 Proton/peptide symporter family protein from Vibrio cholerae (514 aa). FASTA: opt: 1234 Z-score: 1376.6 E(): 8.7e-69 Smith-Waterman score: 1245; 42.050identity in 478 aa overlap. ORF ftt1253; proton-dependent oligopeptide transport (POT) family protein 1273219..1274706 Francisella tularensis subsp. tularensis SCHU S4 3191476 YP_170212.1 CDS fadD1 NC_006570.2 1274814 1276505 D Similar to Q89UU0 Long-chain-fatty-acid-CoA ligase from Bradyrhizobium japonicum (561 aa). FASTA: opt: 1308 Z-score: 1621.3 E(): 2.1e-82 Smith-Waterman score: 1308; 38.324 identity in 561 aa overlap.; acyl-CoA synthetase 1274814..1276505 Francisella tularensis subsp. tularensis SCHU S4 3191870 YP_170213.1 CDS FTT_1255c NC_006570.2 1276493 1277341 R Similar to Q8PAT1 Transcriptional regulator from Xanthomonas campestris (pv. campestris) (287 aa). FASTA: opt: 689 Z-score: 866.5 E(): 2.3e-40 Smith-Waterman score: 689; 37.354identity in 257 aa overlap. ORF ftt1255c; AraC family transcriptional regulator complement(1276493..1277341) Francisella tularensis subsp. tularensis SCHU S4 3191460 YP_170214.1 CDS emrB NC_006570.2 1277485 1279014 D Similar to Q83DE1 Drug resistance transporter, EmrB/QacA family from Coxiella burnetii (526 aa). FASTA: opt: 1037 Z-score: 1145.4 E(): 6.6e-56 Smith-Waterman score: 1037; 31.928 identity in 498 aa overlap.; major facilitator transporter 1277485..1279014 Francisella tularensis subsp. tularensis SCHU S4 3191559 YP_170215.1 CDS emrA1 NC_006570.2 1279036 1280058 D Similar to Q83DE0 Multidrug resistance protein from Coxiella burnetii (331 aa). FASTA: opt: 742 Z-score: 834.3 E(): 1.4e-38 Smith-Waterman score: 742; 37.695 identity in 321 aa overlap.; HlyD family secretion protein 1279036..1280058 Francisella tularensis subsp. tularensis SCHU S4 3192360 YP_170216.1 CDS FTT_1258 NC_006570.2 1280068 1281540 D Similar to Q93NK3 outer membrane protein from Pseudomonas syringae (pv. syringae) (475 aa). FASTA: opt: 594 Z-score: 697.2 E(): 6e-31 Smith-Waterman score: 594; 28.970 identity in 466 aa overlap. ORF ftt1258; outer membrane efflux protein 1280068..1281540 Francisella tularensis subsp. tularensis SCHU S4 3191474 YP_170217.1 CDS nadE NC_006570.2 1281669 1282418 D Similar to NADE_MYCPN (P75216) Probable NH(3)-dependent NAD(+) synthetase Mycoplasma pneumoniae (248 aa). FASTA: opt: 601 Z-score: 760.1 E(): 1.9e-34 Smith-Waterman score: 601; 40.928identity in 237 aa overlap.; NH(3)-dependent NAD(+) synthetase 1281669..1282418 Francisella tularensis subsp. tularensis SCHU S4 3190905 YP_170218.1 CDS FTT_1260 NC_006570.2 1282522 1282944 D ORF ftt1260; hypothetical protein 1282522..1282944 Francisella tularensis subsp. tularensis SCHU S4 3191472 YP_170219.1 CDS isftu1 NC_006570.2 1283965 1284749 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(1283965..1284291,1284291..1284749) Francisella tularensis subsp. tularensis SCHU S4 3190916 YP_170220.1 CDS FTT_1263c NC_006570.2 1285041 1286036 R Duplicate of FTT0354 and FTT0378. ORF ftt1263c; hypothetical protein complement(1285041..1286036) Francisella tularensis subsp. tularensis SCHU S4 3191465 YP_170221.1 CDS isftu1 NC_006570.2 1286965 1287749 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(1286965..1287423,1287423..1287749)) Francisella tularensis subsp. tularensis SCHU S4 3190884 YP_170222.1 CDS yhhW NC_006570.2 1287773 1288486 R Similar to YC10_PSEAE (Q9I4C8) Hypothetical protein PA1210 from Pseudomonas aeruginosa (232 aa). FASTA: opt: 602 Z-score: 765.5 E(): 9.5e-35 Smith-Waterman score: 602; 41.667 identity in 228 aa overlap. This CDS is disrupted by the insertion of an ISFtu1 element occurring very near the C-terminal end. It is not clear whether this insertion affects the function of the protein.; pirin complement(1287773..1288486) Francisella tularensis subsp. tularensis SCHU S4 3191272 YP_170223.1 CDS FTT_1267 NC_006570.2 1288586 1289482 D Similar to Q8E7Z8 Transcriptional regulator, LysR family from Shewanella oneidensis (289 aa). FASTA: opt: 561 Z-score: 683.5 E(): 3.5e-30 Smith-Waterman score: 561; 32.423identity in 293 aa overlap. ORF ftt1267; LysR family transcriptional regulator 1288586..1289482 Francisella tularensis subsp. tularensis SCHU S4 3191463 YP_170224.1 CDS dnaJ1 NC_006570.2 1289496 1290620 R chaperone Hsp40; co-chaperone with DnaK; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion; molecular chaperone DnaJ complement(1289496..1290620) Francisella tularensis subsp. tularensis SCHU S4 3191529 YP_170225.1 CDS dnaK NC_006570.2 1290709 1292637 R heat shock protein 70; assists in folding of nascent polypeptide chains; refolding of misfolded proteins; utilizes ATPase activity to help fold; co-chaperones are DnaJ and GrpE; multiple copies in some bacteria; molecular chaperone DnaK complement(1290709..1292637) Francisella tularensis subsp. tularensis SCHU S4 3191531 YP_170226.1 CDS grpE NC_006570.2 1292738 1293325 R with DnaK and DnaJ acts in response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins; may act as a thermosensor; heat shock protein GrpE complement(1292738..1293325) Francisella tularensis subsp. tularensis SCHU S4 3191601 YP_170227.1 CDS mltA NC_006570.2 1293414 1294580 D Similar to Q81ZZ6 membrane-bound lytic murein transglycosylase A transmembrane protein from Nitrosomonas europaea (440 aa). FASTA: opt: 821 Z-score: 989.7 E(): 3.1e-47 Smith-Waterman score: 821; 39.769 identity in 347 aa overlap.; membrane-bound lytic murein transglycosylase A (MLT) family protein 1293414..1294580 Francisella tularensis subsp. tularensis SCHU S4 3191995 YP_170228.1 CDS FTT_1272 NC_006570.2 1294573 1295757 D Similar to Q8KB91 Protoporphyrinogen oxidase, from Chlorobium tepidum (399 aa). FASTA: opt: 594 Z-score: 698.0 E(): 5.5e-31 Smith-Waterman score: 673; 31.552 identity in 393 aa overlap. ORF ftt1272; hypothetical protein 1294573..1295757 Francisella tularensis subsp. tularensis SCHU S4 3191462 YP_170229.3 CDS rplM NC_006570.2 1295869 1296324 D in Escherichia coli this protein is one of the earliest assembly proteins in the large subunit; 50S ribosomal protein L13 1295869..1296324 Francisella tularensis subsp. tularensis SCHU S4 3192012 YP_170230.2 CDS rpsI NC_006570.2 1296340 1296729 D forms a direct contact with the tRNA during translation; 30S ribosomal protein S9 1296340..1296729 Francisella tularensis subsp. tularensis SCHU S4 3192545 YP_170231.1 CDS mglA NC_006570.2 1296789 1297406 D Similar to O52734 Macrophage growth locus A from Francisella novicida strain U112 (205 aa). FASTA: opt: 1275 Z-score: 1587.7 E(): 1.5e-80 Smith-Waterman score: 1275; 99.512 identity in 205 aa overlap (1-205:1-205).; macrophage growth locus subunit A 1296789..1297406 Francisella tularensis subsp. tularensis SCHU S4 3192495 YP_170232.1 CDS mglB NC_006570.2 1297408 1297815 D Identical to O52735 Macrophage growth locus B from Francisella novicida strain U112 (135 aa).; ClpXP protease specificity-enhancing factor 1297408..1297815 Francisella tularensis subsp. tularensis SCHU S4 3191082 YP_170233.1 CDS FTT_1277c NC_006570.2 1297812 1299083 R Similar to Q88EE1 Sodium-solute symporter, from Pseudomonas putida (strain KT2440) (459 aa). FASTA: opt: 279 Z-score: 311.8 E(): 1.8e-09 Smith-Waterman score: 300; 20.863identity in 417 aa overlap. ORF ftt1277c; sodium-solute symporter family protein complement(1297812..1299083) Francisella tularensis subsp. tularensis SCHU S4 3191458 YP_170234.1 CDS rnhB NC_006570.2 1299076 1299639 R RNH2; RNase HII; binds manganese; endonuclease which specifically degrades the RNA of RNA-DNA hybrids; ribonuclease HII complement(1299076..1299639) Francisella tularensis subsp. tularensis SCHU S4 3191604 YP_170235.1 CDS yfhQ NC_006570.2 1299641 1300384 R Similar to Q8ZCR9 SpoU-family rRNA methylase from Yersinia pestis (257 aa). FASTA: opt: 688 Z-score: 847.8 E(): 2.5e-39 Smith-Waterman score: 688; 47.755 identity in 245 aa overlap.; SpoU rRNA methylase family protein complement(1299641..1300384) Francisella tularensis subsp. tularensis SCHU S4 3192166 YP_170236.1 CDS ptsN NC_006570.2 1300377 1300832 R Similar to Q88PA0 Phosphotransferase system, nitrogen regulatory IIA protein from Pseudomonas putida (strain KT2440) (154 aa). FASTA: opt: 239 Z-score: 318.2 E(): 7.8e-10 Smith-Waterman score: 239; 26.316identity in 152 aa overlap.; PEP-dependent sugar PTS system family protein complement(1300377..1300832) Francisella tularensis subsp. tularensis SCHU S4 3191252 YP_170237.1 CDS yhbH NC_006570.2 1300869 1301165 R Similar to RP5M_PSEPK (P15592) Probable sigma(54) modulation protein from Pseudomonas putida (102 aa). FASTA: opt: 336 Z-score: 439.6 E(): 1.4e-16 Smith-Waterman score: 336; 54.839 identity in 93 aa overlap.; sigma-54 modulation protein complement(1300869..1301165) Francisella tularensis subsp. tularensis SCHU S4 3191268 YP_170238.1 CDS FTT_1282 NC_006570.2 1301284 1301529 D ORF ftt1282; hypothetical protein 1301284..1301529 Francisella tularensis subsp. tularensis SCHU S4 3191455 YP_170239.1 CDS trmE NC_006570.2 1301535 1302887 D in Escherichia coli this protein is involved in the biosynthesis of the hypermodified nucleoside 5-methylaminomethyl-2-thiouridine, which is found in the wobble position of some tRNAs and affects ribosomal frameshifting; shows potassium-dependent dimerization and GTP hydrolysis; also involved in regulation of glutamate-dependent acid resistance and activation of gadE; tRNA modification GTPase TrmE 1301535..1302887 Francisella tularensis subsp. tularensis SCHU S4 3192196 YP_170240.1 CDS FTT_1284c NC_006570.2 1302897 1303091 R ORF ftt1284c; hypothetical protein complement(1302897..1303091) Francisella tularensis subsp. tularensis SCHU S4 3190792 YP_170241.1 CDS FTT_1285c NC_006570.2 1303123 1304013 R Similar to Q8D448 Transcriptional regulator from Vibrio vulnificus (303 aa). FASTA: opt: 372 Z-score: 436.1 E(): 2.1e-16 Smith-Waterman score: 372; 28.571 identity in 294 aa overlap. ORF ftt1285c; LysR family transcriptional regulator complement(1303123..1304013) Francisella tularensis subsp. tularensis SCHU S4 3190789 YP_170242.1 CDS cbs NC_006570.2 1305421 1306344 D Similar to Q9BH24 Cystathionine beta-synthase 5 from Trypanosoma cruzi (384 aa). FASTA: opt: 540 Z-score: 651.0 E(): 2.3e-28 Smith-Waterman score: 542; 31.494 identity in 308 aa overlap.; cystathionine beta-synthase 1305421..1306344 Francisella tularensis subsp. tularensis SCHU S4 3191274 YP_170243.1 CDS FTT_1288 NC_006570.2 1306598 1307167 D Similar to Q8Z651 membrane protein from Salmonella typhi (204 aa). FASTA: opt: 462 Z-score: 553.9 E(): 5.9e-23 Smith-Waterman score: 462; 36.667 identity in 180 aa overlap. ORF ftt1288; hypothetical protein 1306598..1307167 Francisella tularensis subsp. tularensis SCHU S4 3191091 YP_170245.1 CDS metG NC_006570.2 1314025 1316049 D methionine--tRNA ligase; MetRS; adds methionine to tRNA(Met) with cleavage of ATP to AMP and diphosphate; some MetRS enzymes form dimers depending on a C-terminal domain that is also found in other proteins such as Trbp111 in Aquifex aeolicus and the cold-shock protein CsaA from Bacillus subtilis while others do not; four subfamilies exist based on sequence motifs and zinc content; methionyl-tRNA synthetase 1314025..1316049 Francisella tularensis subsp. tularensis SCHU S4 3192497 YP_170246.1 CDS FTT_1291 NC_006570.2 1316015 1317289 D Similar to EXUT_BACSU (O34456) Hexuronate transporter from Bacillus subtilis (422 aa). FASTA: opt: 655 Z-score: 716.7 E(): 5e-32 Smith-Waterman score: 659; 28.250 identity in 400 aa overlap. ORF ftt1291; major facilitator transporter 1316015..1317289 Francisella tularensis subsp. tularensis SCHU S4 3191093 YP_170247.1 CDS FTT_1292c NC_006570.2 1317286 1317888 R Similar to Q88YM1 Hypothetical protein from Lactobacillus plantarum (215 aa). FASTA: opt: 420 Z-score: 532.1 E(): 9.5e-22 Smith-Waterman score: 420; 35.025 identity in 197 aa overlap. ORF ftt1292c; hypothetical protein complement(1317286..1317888) Francisella tularensis subsp. tularensis SCHU S4 3191094 YP_170248.1 CDS FTT_1293c NC_006570.2 1317938 1318552 R Similar to Q9KSS8 Hypothetical protein VC1178 from Vibrio cholerae (206 aa). FASTA: opt: 558 Z-score: 715.6 E(): 5.8e-32 Smith-Waterman score: 558; 42.703 identity in 185 aa overlap. ORF ftt1293c; Sua5_yciO_yrdC family protein complement(1317938..1318552) Francisella tularensis subsp. tularensis SCHU S4 3191096 YP_170249.1 CDS ispZ NC_006570.2 1318613 1319143 D Similar to ISPZ_RALSO (Q8XYL2) Probable intracellular septation protein from Ralstonia solanacearum (Pseudomonas solanacearum) (186 aa). FASTA: opt: 549 Z-score: 659.0 E(): 8.2e-29 Smith-Waterman score: 549; 38.150 identity in 173 aa overlap.; intracellular septation protein A family protein 1318613..1319143 Francisella tularensis subsp. tularensis SCHU S4 3192218 YP_170250.1 CDS glk1 NC_006570.2 1319482 1320498 R Similar to GLK_ANASP (P58616) Glucokinase from Anabaena sp. (strain PCC 7120) (342 aa). FASTA: opt: 735 Z-score: 904.6 E(): 1.7e-42 Smith-Waterman score: 735; 36.735 identity in 343 aa overlap.; glucose kinase complement(1319482..1320498) Francisella tularensis subsp. tularensis SCHU S4 3192511 YP_170251.1 CDS ubiE NC_006570.2 1320592 1321344 D Similar to Q8D1I3 COQ5 methyltransferase from Yersinia pestis (251 aa). FASTA: opt: 1184 Z-score: 1440.7 E(): 2.4e-72 Smith-Waterman score: 1184; 70.800 identity in 250 aa overlap.; menaquinone biosynthesis methyltransferase 1320592..1321344 Francisella tularensis subsp. tularensis SCHU S4 3192321 YP_170252.1 CDS FTT_1297 NC_006570.2 1321328 1321933 D ORF ftt1297; hypothetical protein 1321328..1321933 Francisella tularensis subsp. tularensis SCHU S4 3191097 YP_170253.1 CDS ubiB NC_006570.2 1321926 1323584 D Similar to Q83A88 Ubiquinone biosynthesis protein AarF, from Coxiella burnetii (541 aa). FASTA: opt: 1682 Z-score: 2006.3 E(): 7.3e-104 Smith-Waterman score: 1682; 48.269 identity in 520 aa overlap. unrelated to the ABC transporter proteins.; 2-polyprenylphenol 6-hydroxylase 1321926..1323584 Francisella tularensis subsp. tularensis SCHU S4 3190824 YP_170254.1 CDS hitA NC_006570.2 1323590 1323928 D Similar to Q82WM8 HIT (Histidine triad) family from Nitrosomonas europaea (116 aa). FASTA: opt: 424 Z-score: 574.5 E(): 4.1e-24 Smith-Waterman score: 424; 53.571 identity in 112 aa overlap.; histidine triad (HIT) family protein 1323590..1323928 Francisella tularensis subsp. tularensis SCHU S4 3192431 YP_170255.1 CDS FTT_1300c NC_006570.2 1323945 1324076 R ORF ftt1300c; hypothetical protein complement(1323945..1324076) Francisella tularensis subsp. tularensis SCHU S4 3191100 YP_170256.1 CDS FTT_1302 NC_006570.2 1325075 1325905 D ORF ftt1302; hypothetical protein 1325075..1325905 Francisella tularensis subsp. tularensis SCHU S4 3191106 YP_170257.1 CDS FTT_1303c NC_006570.2 1325907 1326782 R ORF ftt1303c; hypothetical protein complement(1325907..1326782) Francisella tularensis subsp. tularensis SCHU S4 3191105 YP_170258.1 CDS murB NC_006570.2 1326861 1327709 R Similar to MURB_THEMA (Q9X239) UDP-N-acetylenolpyruvoylglucosamine reductase from Thermotoga maritima (284 aa). FASTA: opt: 577 Z-score: 699.7 E(): 4.4e-31 Smith-Waterman score: 577; 39.273 identity in 275 aa overlap.; UDP-N-acetylenolpyruvoylglucosamine reductase complement(1326861..1327709) Francisella tularensis subsp. tularensis SCHU S4 3191254 YP_170259.1 CDS murA NC_006570.2 1327717 1329051 R Similar to Q814X9 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Bacillus cereus (strain ATCC 14579 / DSM 31) (434 aa). FASTA: opt: 697 Z-score: 859.3 E(): 5.6e-40 Smith-Waterman score: 765; 33.333 identity in 438 aa overlap.; UDP-N-acetylglucosamine 1-carboxyvinyltransferase complement(1327717..1329051) Francisella tularensis subsp. tularensis SCHU S4 3191251 YP_170260.1 CDS engA NC_006570.2 1328990 1330387 R EngA; essential Neisserial GTPase; synchronizes cellular events by interacting with multiple targets with tandem G-domains; overexpression in Escherichia coli suppresses rrmJ mutation; structural analysis of the Thermotoga maritima ortholog shows different nucleotide binding affinities in the two binding domains; GTP-binding protein EngA complement(1328990..1330387) Francisella tularensis subsp. tularensis SCHU S4 3191567 YP_170261.1 CDS FTT_1307c NC_006570.2 1330603 1331139 R ORF ftt1307c; hypothetical protein complement(1330603..1331139) Francisella tularensis subsp. tularensis SCHU S4 3191107 YP_170262.1 CDS FTT_1308c NC_006570.2 1331184 1331792 R ORF ftt1308c; hypothetical protein complement(1331184..1331792) Francisella tularensis subsp. tularensis SCHU S4 3191108 YP_170263.1 CDS hflB NC_006570.2 1332931 1334847 R Similar to FTSH_ECO57 (Q8X9L0) Cell division protease ftsH from Escherichia coli O157:H7 (644 aa). FASTA: opt: 2569 Z-score: 2823.5 E(): 2.2e-149 Smith-Waterman score: 2569; 60.125 identity in 642 aa overlap.; ATP-dependent metalloprotease complement(1332931..1334847) Francisella tularensis subsp. tularensis SCHU S4 3192424 YP_170264.1 CDS yieO NC_006570.2 1335070 1336476 D Similar to Q8UHQ9 MFS permease from Agrobacterium tumefaciens (strain C58 / ATCC 33970) (475 aa). FASTA: opt: 757 Z-score: 760.1 E(): 1.9e-34 Smith-Waterman score: 766; 32.735 identity in 446 aa overlap.; major facilitator transporter 1335070..1336476 Francisella tularensis subsp. tularensis SCHU S4 3191279 YP_170265.1 CDS uvrA NC_006570.2 1336482 1339304 R Similar to UVRA_PSEAE (Q9HWG0) UvrABC system protein A (UvrA protein) from Pseudomonas aeruginosa (945 aa). FASTA: opt: 4062 Z-score: 4339.5 E():0 Smith-Waterman score: 4062; 63.800identity in 942 aa overlap.; DNA excision repair protein subunit A complement(1336482..1339304) Francisella tularensis subsp. tularensis SCHU S4 3191913 YP_170266.1 CDS greA NC_006570.2 1339343 1339825 R necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites; arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus; transcription elongation factor GreA complement(1339343..1339825) Francisella tularensis subsp. tularensis SCHU S4 3191923 YP_170267.1 CDS FTT_1314c NC_006570.2 1339859 1340443 R Similar to Q8EBJ4 Type IV pilus biogenesis protein (164 aa). FASTA: opt: 157 Z-score: 204.8 E(): 0.0016 Smith-Waterman score: 157; 27.607 identity in 163 aa overlap. The pili fiber building block protein has a highly distinct primary structure; a short positively charged leader sequence, and a highly conserved and highly hydrophobic amino-terminal domain which forms the core of the pilus fiber; A variety of studies suggest that the invariant glycine (G) at -1 is required for prepilin cleavage and assembly and that the glutamate (E) at + 5 is essential for assembly and efficient N-terminal methylation but not cleavage. The region near the carboxy terminus is often hydrophilic and is stabilized by one or two disulfide bridges that may be involved in recognition of receptors. The genes with these characteristics are usually termed pilA, pilE, pilV, pilW, pilX and FimU. (Mattick John S.. 'Type IV Pili and twitching motility'. Annu. Rev. Microbiol. 2002. 56:289-314.) This protein has all the characteristics described above, except the glutamate (E) at + 5, but shows a weak global homology. ORF ftt1314c; Type IV pili fiber building block protein complement(1339859..1340443) Francisella tularensis subsp. tularensis SCHU S4 3191109 YP_170268.1 CDS pgi NC_006570.2 1340444 1342066 R functions in sugar metabolism in glycolysis and the Embden-Meyerhof pathways (EMP) and in gluconeogenesis; catalyzes reversible isomerization of glucose-6-phosphate to fructose-6-phosphate; member of PGI family; glucose-6-phosphate isomerase complement(1340444..1342066) Francisella tularensis subsp. tularensis SCHU S4 3191335 YP_170269.1 CDS FTT_1316c NC_006570.2 1342053 1342706 R Similar to Q8R9T9 Thiamine pyrophosphokinase from Thermoanaerobacter tengcongensis (211 aa). FASTA: opt: 221 Z-score: 278.6 E(): 1.3e-07 Smith-Waterman score: 238; 25.943 identity in 212 aa overlap. ORF ftt1316c; thiamine pyrophospokinase complement(1342053..1342706) Francisella tularensis subsp. tularensis SCHU S4 3191111 YP_170270.1 CDS guaB NC_006570.2 1342772 1344232 R Similar to Q88P22 Inosine-5-monophosphate dehydrogenase from Pseudomonas putida (strain KT2440) (489 aa). FASTA: opt: 2187 Z-score: 2403.4 E(): 5.6e-126 Smith-Waterman score: 2187; 68.033 identity in 488 aa overlap.; inosine-5'-monophosphate dehydrogenase complement(1342772..1344232) Francisella tularensis subsp. tularensis SCHU S4 3192330 YP_170271.1 CDS pepA NC_006570.2 1344333 1345772 R Similar to Q887M0 Cytosol aminopeptidase from Pseudomonas syringae (pv. tomato) (496 aa). FASTA: opt: 1161 Z-score: 1388.1 E(): 2e-69 Smith-Waterman score: 1165; 42.540 identity in 496 aa overlap.; cytosol aminopeptidase complement(1344333..1345772) Francisella tularensis subsp. tularensis SCHU S4 3191324 YP_170272.1 CDS FTT_1319 NC_006570.2 1345938 1347020 D Similar to Q8EH61 Conserved hypothetical protein from Shewanella oneidensis (370 aa). FASTA: opt: 370 Z-score: 433.4 E(): 3e-16 Smith-Waterman score: 370; 21.508 identity in 358 aa overlap. ORF ftt1319; YjgP/YjgQ family permease 1345938..1347020 Francisella tularensis subsp. tularensis SCHU S4 3191112 YP_170273.1 CDS FTT_1320 NC_006570.2 1347020 1348081 D Similar to Q8ZK26 permease from Salmonella typhimurium (360 aa). FASTA: opt: 510 Z-score: 585.0 E(): 1.1e-24 Smith-Waterman score: 511; 27.147 identity in 361 aa overlap. ORF ftt1320; YjgP/YjgQ family permease 1347020..1348081 Francisella tularensis subsp. tularensis SCHU S4 3191114 YP_170274.1 CDS FTT_1321 NC_006570.2 1348089 1349342 D Similar to Q83AI4 Peptidase, M16 family from Coxiella burnetii (459 aa). FASTA: opt: 868 Z-score: 1053.1 E(): 9.1e-51 Smith-Waterman score: 868; 34.158 identity in 404 aa overlap. ORF ftt1321; peptidase M16 family protein 1348089..1349342 Francisella tularensis subsp. tularensis SCHU S4 3191116 YP_170275.1 CDS FTT_1322 NC_006570.2 1349349 1350572 D Similar to Q83AI5 Peptidase, M16 family from Coxiella burnetii (443 aa). FASTA: opt: 605 Z-score: 727.1 E(): 1.3e-32 Smith-Waterman score: 605; 28.607 identity in 402 aa overlap. ORF ftt1322; peptidase M16 family protein 1349349..1350572 Francisella tularensis subsp. tularensis SCHU S4 3191117 YP_170276.1 CDS FTT_1323 NC_006570.2 1350573 1351151 D Similar to YHHF_HAEIN (P44869) methylase HI0767 from Haemophilus influenzae (193 aa). FASTA: opt: 455 Z-score: 588.3 E(): 7e-25 Smith-Waterman score: 455; 42.697 identity in 178 aa overlap. ORF ftt1323; methylase 1350573..1351151 Francisella tularensis subsp. tularensis SCHU S4 3191119 YP_170277.1 CDS FTT_1324 NC_006570.2 1351185 1351835 D Similar to Q9CM99 Hypothetical protein PM0935 from Pasteurella multocida (226 aa). FASTA: opt: 523 Z-score: 599.4 E(): 1.7e-25 Smith-Waterman score: 523; 39.151 identity in 212 aa overlap. ORF ftt1324; hypothetical protein 1351185..1351835 Francisella tularensis subsp. tularensis SCHU S4 3191120 YP_170278.1 CDS miaE NC_006570.2 1351888 1352499 R Similar to Q88KV1 TRNA-(ms(2)io(6)a)-hydroxylase, from Pseudomonas putida (strain KT2440)(205 aa). FASTA: opt: 660 Z-score: 867.5 E(): 2e-40 Smith-Waterman score: 660; 50.777 identity in 193 aa overlap.; tRNA-(ms(2)io(6)a)-hydroxylase complement(1351888..1352499) Francisella tularensis subsp. tularensis SCHU S4 3190883 YP_170279.1 CDS udp NC_006570.2 1352615 1353418 D Similar to Q9CKN8 Udp from Pasteurella multocida (252 aa). FASTA: opt: 817 Z-score: 1065.9 E(): 1.8e-51 Smith-Waterman score: 817; 48.760 identity in 242 aa overlap.; uridine phosphorylase 1352615..1353418 Francisella tularensis subsp. tularensis SCHU S4 3192475 YP_170280.1 CDS cdd NC_006570.2 1353439 1353840 D Similar to Q8RCG7 Cytidine deaminase from Thermoanaerobacter tengcongensis (135 aa). FASTA: opt: 413 Z-score: 565.4 E(): 1.3e-23 Smith-Waterman score: 413; 52.273 identity in 132 aa overlap.; cytidine deaminase 1353439..1353840 Francisella tularensis subsp. tularensis SCHU S4 3190961 YP_003097805.1 CDS FTT_1328c NC_006570.2 1354038 1357067 R Similar to Q8EDV0 Oxidoreductase,FAD-binding, from Shewanella oneidensis (1013 aa).; FAD-binding family protein complement(1354038..1357067) Francisella tularensis subsp. tularensis SCHU S4 3191121 YP_170281.1 CDS gpmI NC_006570.2 1357287 1358825 D catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate; phosphoglyceromutase 1357287..1358825 Francisella tularensis subsp. tularensis SCHU S4 3190945 YP_170282.1 CDS serS NC_006570.2 1358840 1360120 D catalyzes a two-step reaction, first charging a serine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; seryl-tRNA synthetase 1358840..1360120 Francisella tularensis subsp. tularensis SCHU S4 3190714 YP_170283.1 CDS cscK NC_006570.2 1360230 1361129 D Similar to Q97IW7 Fructokinase from Clostridium acetobutylicum (288 aa). FASTA: opt: 932 Z-score: 1175.6 E(): 1.4e-57 Smith-Waterman score: 932; 47.603identity in 292 aa overlap.; fructokinase 1360230..1361129 Francisella tularensis subsp. tularensis SCHU S4 3191896 YP_170284.1 CDS FTT_1332 NC_006570.2 1361207 1362466 D Similar to Q9X4C7 Repeat unit exporter from Escherichia coli (419 aa). FASTA: opt: 324 Z-score: 369.2 E(): 1.1e-12 Smith-Waterman score: 324; 22.604 identity in 407 aa overlap. ORF ftt1332; polysaccharide biosynthesis protein 1361207..1362466 Francisella tularensis subsp. tularensis SCHU S4 3191123 YP_170285.1 CDS FTT_1333c NC_006570.2 1362470 1362670 R ORF ftt1333c; hypothetical protein complement(1362470..1362670) Francisella tularensis subsp. tularensis SCHU S4 3191125 YP_170286.1 CDS FTT_1334c NC_006570.2 1362698 1363207 R ORF ftt1334c; hypothetical protein complement(1362698..1363207) Francisella tularensis subsp. tularensis SCHU S4 3191128 YP_170287.1 CDS cydD NC_006570.2 1363584 1365365 D in Escherichia coli the CydCD ABC transporter exports cysteine and glutathione into the periplasm in order to maintain redox balance; important for cytochrome bd and c; cysteine/glutathione ABC transporter permease/ATP-binding protein 1363584..1365365 Francisella tularensis subsp. tularensis SCHU S4 3191909 YP_170288.1 CDS cydC NC_006570.2 1365362 1367011 D in Escherichia coli the CydCD ABC transporter exports cysteine and glutathione into the periplasm in order to maintain redox balance; important for cytochrome bd and c; cysteine/glutathione ABC transporter permease/ATP-binding protein 1365362..1367011 Francisella tularensis subsp. tularensis SCHU S4 3191906 YP_170289.1 CDS dctA NC_006570.2 1367013 1368263 R Similar to DTA2_PSEAE (Q9I4F5) C4-dicarboxylate transport protein from Pseudomonas aeruginosa (436 aa). FASTA: opt: 1534 Z-score: 1703.1 E(): 5.7e-87 Smith-Waterman score: 1534; 55.206identity in 413 aa overlap.; C4-dicarboxylate transport protein complement(1367013..1368263) Francisella tularensis subsp. tularensis SCHU S4 3191503 YP_170290.1 CDS FTT_1338c NC_006570.2 1368402 1368782 R Similar to Q99WA6 SA0455 protein (Translation initiation inhibitor homologue) from Staphylococcus aureus (126 aa). FASTA: 452 opt: 452 Z-score: 597.7 E(): 2.1e-25 Smith-Waterman score: 452; 61.017 identity in 118 aa overlap. ORF ftt1338c; translation initiation inhibitor complement(1368402..1368782) Francisella tularensis subsp. tularensis SCHU S4 3191130 YP_170291.1 CDS FTT_1339c NC_006570.2 1368782 1370326 R Similar to Q87N13 Sulfate permease family protein from Vibrio parahaemolyticus (519 aa). FASTA: opt: 1333 Z-score: 1472.3 E(): 4.1e-74 Smith-Waterman score: 1602; 47.398 identity in 538 aa overlap. ORF ftt1339c; sulfate permease family protein complement(1368782..1370326) Francisella tularensis subsp. tularensis SCHU S4 3191572 YP_170292.1 CDS isftu2 NC_006570.2 1370445 1371188 R ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 transposase from Francisella tularensis (247 aa).; transposase complement(1370445..1371188) Francisella tularensis subsp. tularensis SCHU S4 3192307 YP_170293.1 CDS FTT_1341 NC_006570.2 1371389 1372045 D Similiar to CAD85637 (Q82TY8) integral membrane transmembrane protein from Nitrosomonas europaea (221 aa). FASTA: 670 Z-score: 817.1 E(): 1.2e-37 50.226identity in 221 aa overlap ORF ftt1341; hypothetical protein 1371389..1372045 Francisella tularensis subsp. tularensis SCHU S4 3191574 YP_170294.1 CDS FTT_1342 NC_006570.2 1372159 1373214 D Similar to Q08861 Gamma-aminobutyric acid-grated chloride- ion channel/receptor, zeta subunit, precusor from Lymnaea stagnalis (437 aa). FASTA: opt: 357 Z-score: 407.4 E(): 7.7e-15 Smith-Waterman score: 360; 23.510 identity in 302 aa overlap ORF ftt1342; hypothetical protein 1372159..1373214 Francisella tularensis subsp. tularensis SCHU S4 3191575 YP_170295.1 CDS FTT_1343c NC_006570.2 1373509 1374318 R ORF ftt1343c; hypothetical protein complement(1373509..1374318) Francisella tularensis subsp. tularensis SCHU S4 3191576 YP_170296.1 CDS pdpA1 NC_006570.2 1379925 1382387 D Similar to AAP58968.1 (Q7X3I9) PdpA from Francisella novicida (820 aa). BLAST Score = 1545 bits (4000), Expect = 0.0 Identities = 777/820 (94), Positives = 787/820 (95) Identical to FTT1699. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625; hypothetical protein 1379925..1382387 Francisella tularensis subsp. tularensis SCHU S4 3191294 YP_170297.1 CDS pdpB1 NC_006570.2 1382392 1385673 D Similar to AAP58967.1 (Q7X3J0) PdpB from Francisella novicida (1093 aa). BLAST Score = 1914 bits (4959), Expect = 0.0 Identities = 975/1093 (89),Positives = 988/1093 (90) Identical to FTT1700. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625; hypothetical protein 1382392..1385673 Francisella tularensis subsp. tularensis SCHU S4 3191301 YP_170298.1 CDS FTT_1346 NC_006570.2 1385663 1386040 D Identical to FTT1701. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625 ORF ftt1346; hypothetical protein 1385663..1386040 Francisella tularensis subsp. tularensis SCHU S4 3191570 YP_170299.1 CDS FTT_1347 NC_006570.2 1386053 1386547 D Similar to AAP58969.1 (Q7XI38) unknown protein from Francisella novicida (164 aa). BLAST Score = 305 bits (780), Expect = 2e-82 Identities = 163/164 (99),Positives = 163/164 (99) Identical to FTT1702. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625 ORF ftt1347; hypothetical protein 1386053..1386547 Francisella tularensis subsp. tularensis SCHU S4 3191577 YP_170300.1 CDS FTT_1348 NC_006570.2 1386552 1388282 D 1-213 aa similar to AAP58970.1 (Q7X3I7) unknown Francisella novicida (212 aa). BLAST Score 352 ,Expect = 1e-95 Identities = 177/209 (84), Positives = 189/209 (90). 330-580aa similar to AAP58971.1 (Q7X3I6) unknown protein from Francisella tularensis subsp. novicida (251 aa). BLAST Score = 483 bits (1244), Expect = e-135 Identities = 243/251 (96), Positives = 249/251 (99). Identical to FTT1703. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625. Protein fusion product of two hypothetical proteins in F. novicida. ORF ftt1348; hypothetical protein 1386552..1388282 Francisella tularensis subsp. tularensis SCHU S4 3191578 YP_170301.1 CDS FTT_1349 NC_006570.2 1388283 1388804 D Similar to AAP58972.1 (Q7X3I5) unknown protein from Francisella novicida (173 aa). BLAST Score = 336, Expect = 1e-91 Identities = 170/173 (98), Positives = 171/173 (98) Identical to FTT1704. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625 ORF ftt1349; hypothetical protein 1388283..1388804 Francisella tularensis subsp. tularensis SCHU S4 3191580 YP_170302.1 CDS FTT_1350 NC_006570.2 1388817 1390247 D Similar to AAP58973.1 (Q7X3I4) unknown protein from Francisella novicida (476 aa). BLAST Score = 864 bits (2233), Expect = 0.0 Identities = 433/476 (90),Positives = 437/476 (91) Identical to FTT1705. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625 ORF ftt1350; hypothetical protein 1388817..1390247 Francisella tularensis subsp. tularensis SCHU S4 3191581 YP_170303.1 CDS FTT_1351 NC_006570.2 1390260 1390883 D Similar to AAP58974.1 (Q7X3I3) unknown protein from Francisella novicida (207 aa). BLAST Score = 391 bits (1005), Expect = e-108 Identities = 194/207 (93),Positives = 195/207 (94) Identical to FTT1706. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625 ORF ftt1351; hypothetical protein 1390260..1390883 Francisella tularensis subsp. tularensis SCHU S4 3191586 YP_170304.1 CDS FTT_1352 NC_006570.2 1390905 1392056 D Similar to AAP58975.1 (Q7X3I2) unknown protein from Francisella novicida (262 aa), BLAST Score = 443 bits (1140), Expect = e-12 Identities = 229/262 (87),Positives = 235/262 (89) This protein is identical to FTT1707. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625 ORF ftt1352; hypothetical protein 1390905..1392056 Francisella tularensis subsp. tularensis SCHU S4 3191588 YP_170305.1 CDS FTT_1353 NC_006570.2 1392022 1392795 D Similar to AAP58976.1 (Q7X3I1) unknown protein from Francisella novicida (257 aa). BLAST Score = 412, Expect = e-114 Identities = 213/257 (82), Positives = 213/257 (82) Identical to FTT1708. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625. ORF ftt1353; hypothetical protein 1392022..1392795 Francisella tularensis subsp. tularensis SCHU S4 3191589 YP_170306.1 CDS pdpC1 NC_006570.2 1392835 1396821 D Similar to AAP58966.1 PdpC from Francisella novicida (1218 aa). BLAST Score = 2172, Expect = 0.0 Identities = 1089/1220 (89), Positives = 1120/1220 (91),Gaps = 3/1220 (0) Identical to FTT1709. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625; hypothetical protein 1392835..1396821 Francisella tularensis subsp. tularensis SCHU S4 3191593 YP_170307.1 CDS FTT_1355 NC_006570.2 1396839 1397414 D Similar to AAP58977.1 (Q7X3I0) unknown protein from Francisella novicida (188 aa). BLAST Score = 321, Expect = 3e-87 Identities = 156/174 (89), Positives = 162/174 (93) Identical to FTT1710. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625 ORF ftt1355; hypothetical protein 1396839..1397414 Francisella tularensis subsp. tularensis SCHU S4 3191594 YP_170308.1 CDS iglD1 NC_006570.2 1397421 1398626 R Similar to AAP58965.1 (Q7C3J6) IglD from Francisella novicida (398 aa). BLAST Score = 746, Expect = 0.0 Identities = 382/398 (95), Positives = 383/398 (96) Identical to FTT1711. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625; intracellular growth locus subunit D complement(1397421..1398626) Francisella tularensis subsp. tularensis SCHU S4 3191518 YP_170309.1 CDS iglC1 NC_006570.2 1398638 1399273 R Similar to AAP58964.1 (Q7X3J5) IglC (23 kDa protein) from Francisella novicida (211 aa). BLAST Score = 405, Expect = e-112 Identities = 209/211 (99), Positives = 210/211 (99) Identical to FTT1712. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625; intracellular growth locus subunit C complement(1398638..1399273) Francisella tularensis subsp. tularensis SCHU S4 3191591 YP_170310.1 CDS iglB1 NC_006570.2 1399284 1400828 R Similar to AAP58963.1 (Q7X3J4) IglB from Francisella novicida (514 aa). BLAST Score = 997, Expect = 0.0 Identities = 496/514 (96), Positives = 499/514 (97) Identical to FTT1713. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625; intracellular growth locus subunit B complement(1399284..1400828) Francisella tularensis subsp. tularensis SCHU S4 3191564 YP_170311.1 CDS iglA1 NC_006570.2 1400818 1401408 R Similar to AAP58962.1 (Q7X3J5) IglA from Francisella novicida (184 aa). BLAST Score = 350, Expect = 6e-96 Identities = 181/184 (98), Positives = 181/184 (98) Identical to FTT1714. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625; intracellular growth locus subunit A complement(1400818..1401408) Francisella tularensis subsp. tularensis SCHU S4 3191217 YP_170312.1 CDS pdpD1 NC_006570.2 1401453 1405040 R Similar to AAP58961.1 (Q7C3J6) PdpD from Francisella novicida (1245 aa). BLAST Score = 2194, Expect = 0.0 Identities = 1133/1245 (91), Positives = 1154/1245 (92), Gaps = 50/1245 (4) Identical to FTT1715. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625; hypothetical protein complement(1401453..1405040) Francisella tularensis subsp. tularensis SCHU S4 3191310 YP_170313.1 CDS isftu1 NC_006570.2 1407396 1408180 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(1407396..1407854,1407854..1408180)) Francisella tularensis subsp. tularensis SCHU S4 3190873 YP_170314.1 CDS fbaB NC_006570.2 1410206 1411270 R class II aldolase; catalyzes the reversible aldol condensation of dihydroxyacetonephosphate and glyceraldehyde 3-phosphate in the Calvin cycle, glycolysis and gluconeogenesis; fructose-1,6-bisphosphate aldolase complement(1410206..1411270) Francisella tularensis subsp. tularensis SCHU S4 3191878 YP_170315.1 CDS pyk NC_006570.2 1411283 1412719 R Similar to AAO91275 (Q83AU7) Pyruvate kinase from Coxiella burnetii (477 aa). FASTA: opt: 1622 Z-score: 1687.6 E(): 3.8e-86 Smith-Waterman score: 1622; 53.249identity in 477 aa overlap domain, score 55.8, E-value 8.1e-14; pyruvate kinase complement(1411283..1412719) Francisella tularensis subsp. tularensis SCHU S4 3191657 YP_170316.1 CDS pgk NC_006570.2 1412735 1413913 R Converts 3-phospho-D-glycerate to 3-phospho-D-glyceroyl phosphate during the glycolysis pathway; phosphoglycerate kinase complement(1412735..1413913) Francisella tularensis subsp. tularensis SCHU S4 3191360 YP_170317.1 CDS gapA NC_006570.2 1413932 1414978 R Similar to Q8XKT9 Glyceraldehyde-3-phosphate dehydrogenase from Clostridium perfringens (332 aa). FASTA: opt: 1511 Z-score: 1671.9 E(): 2.8e-85 Smith-Waterman score: 1511; 71.341identity in 328 aa overlap; glyceraldehyde-3-phosphate dehydrogenase complement(1413932..1414978) Francisella tularensis subsp. tularensis SCHU S4 3191592 YP_170318.1 CDS tktA NC_006570.2 1414971 1416962 R catalyzes the formation of ribose 5-phosphate and xylulose 5-phosphate from sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate; can transfer ketol groups between several groups; in Escherichia coli there are two tkt genes, tktA expressed during exponential growth and the tktB during stationary phase; transketolase complement(1414971..1416962) Francisella tularensis subsp. tularensis SCHU S4 3190783 YP_170319.1 CDS FTT_1370 NC_006570.2 1417102 1417605 D Similar to AAN42711 (Q83LI4) Orf, conserved hypothetical protein from Shigella flexneri (173 aa). FASTA: opt: 194 Z-score: 254.2 E(): 2.6e-06 Smith-Waterman score: 194; 26.708 identity in 161 aa overlap ORF ftt1370; hypothetical protein 1417102..1417605 Francisella tularensis subsp. tularensis SCHU S4 3191598 YP_170320.1 CDS rpmF NC_006570.2 1417649 1417831 D some L32 proteins have zinc finger motifs consisting of CXXC while others do not; 50S ribosomal protein L32 1417649..1417831 Francisella tularensis subsp. tularensis SCHU S4 3191613 YP_170321.1 CDS plsX NC_006570.2 1417930 1418976 D involved in acylation of glycerol-3-phosphate to form 1-acyl-glycerol-3 phosphate for use in phospholipid biosynthesis; functions with PlsY; glycerol-3-phosphate acyltransferase PlsX 1417930..1418976 Francisella tularensis subsp. tularensis SCHU S4 3191914 YP_170322.1 CDS fabH NC_006570.2 1419073 1420044 D Similar to Q9JW56 3-oxoacyl-[acyl-carrier-protein] synthase III from Neisseria meningitidis (320 aa). FASTA: opt: 1148 Z-score: 1323.1 E(): 7.6e-66 Smith-Waterman score: 1148; 54.859 identity in 319 aa overlap; 3-oxoacyl-ACP synthase 1419073..1420044 Francisella tularensis subsp. tularensis SCHU S4 3191865 YP_170323.1 CDS FTT_1374 NC_006570.2 1420092 1421012 D Similar to Q9CJS7 FabD from Pasteurella multocida (312 aa). FASTA: opt: 984 Z-score: 1099.0 E(): 2.3e-53 Smith-Waterman score: 984;50.163 identity in 307 aa overlap ORF ftt1374; malonyl CoA-ACP transacylase 1420092..1421012 Francisella tularensis subsp. tularensis SCHU S4 3191602 YP_170324.1 CDS fabG NC_006570.2 1421026 1421769 D Similar to Q9CJS6 FabG from Pasteurella multocida (242 aa). FASTA: opt: 1029 Z-score: 1192.2 E(): 1.5e-58 Smith-Waterman score: 1029; 66.667 identity in 240 aa overlap; 3-oxoacyl-ACP reductase 1421026..1421769 Francisella tularensis subsp. tularensis SCHU S4 3191861 YP_170325.1 CDS acpP NC_006570.2 1421865 1422149 D carries the fatty acid chain in fatty acid biosynthesis; acyl carrier protein 1421865..1422149 Francisella tularensis subsp. tularensis SCHU S4 3192405 YP_170326.1 CDS fabF NC_006570.2 1422229 1423488 D Similar to Q8Y0J0 Probable 3-oxoacyl-[acyl-carrier-protein] synthase II from Ralstonia solanacearum (410 aa). FASTA: opt: 1679 Z-score: 1887.3 E(): 2.8e-97 Smith-Waterman score: 1679; 59.233 identity in 417 aa overlap; 3-oxoacyl-ACP synthase 1422229..1423488 Francisella tularensis subsp. tularensis SCHU S4 3191863 YP_008492914.1 CDS FTT_1379c NC_006570.2 1423843 1424529 R hypothetical protein complement(1423843..1424529) Francisella tularensis subsp. tularensis SCHU S4 3191482 YP_170327.1 CDS suhB NC_006570.2 1427069 1427857 D Similar to P22783 Inositol-1-monophosphatase from E. coli (267 aa). FASTA: opt: 630 Z-score: 759.0 E(): 2e-34 Smith-Waterman score: 656; 36.882identity in 263 aa overlap; inositol monophosphatase 1427069..1427857 Francisella tularensis subsp. tularensis SCHU S4 3190744 YP_170328.1 CDS sun NC_006570.2 1427857 1429137 D Similar to P44788 SUN protein (FMU protein) from Haemophilus influenzae (451 aa). FASTA: opt: 956 Z-score: 1079.4 E(): 2.9e-52 Smith-Waterman score: 956; 39.401 identity in 434 aa overlap; Sun protein 1427857..1429137 Francisella tularensis subsp. tularensis SCHU S4 3190743 YP_170329.1 CDS FTT_1384c NC_006570.2 1429115 1429606 R Similar to Q8D9X5 Probable acetyltransferase from Vibrio vulnificus (141 aa). FASTA: opt: 183 Z-score: 242.0 E(): 1.3e-05 Smith-Waterman score: 183; 27.132 identity in 129 aa overlap ORF ftt1384c; acetyltransferase complement(1429115..1429606) Francisella tularensis subsp. tularensis SCHU S4 3191485 YP_170330.2 CDS FTT_1385c NC_006570.2 1429699 1429968 R ORF ftt1385c; hypothetical protein complement(1429699..1429968) Francisella tularensis subsp. tularensis SCHU S4 3191487 YP_170331.1 CDS isftu1 NC_006570.2 1429959 1430743 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(1429959..1430285,1430285..1430743) Francisella tularensis subsp. tularensis SCHU S4 3190917 YP_170332.1 CDS ligN NC_006570.2 1431189 1433225 R Similar to P43813 DNA ligase from Haemophilus influenzae (679 aa). FASTA: opt: 2154 Z-score: 2353.2 E(): 3.2e-123 Smith-Waterman score: 2154; 48.817 identity in 676 aa overlap; DNA ligase complement(1431189..1433225) Francisella tularensis subsp. tularensis SCHU S4 3190767 YP_170333.1 CDS FTT_1388 NC_006570.2 1433533 1434249 D Similar to AAO81619 (Q833S4) Conserved domain protein from Enterococcus faecalis (281 aa). FASTA: opt: 171 Z-score: 214.0 E(): 0.00046 Smith-Waterman score: 194;26.337 identity in 243 aa overlap ORF ftt1388; hypothetical protein 1433533..1434249 Francisella tularensis subsp. tularensis SCHU S4 3191491 YP_170334.1 CDS panB NC_006570.2 1434251 1435045 D catalyzes the formation of tetrahydrofolate and 2-dehydropantoate from 5,10-methylenetetrahydrofolate and 3-methyl-2-oxobutanoate; 3-methyl-2-oxobutanoate hydroxymethyltransferase 1434251..1435045 Francisella tularensis subsp. tularensis SCHU S4 3191227 YP_170335.1 CDS panC NC_006570.2 1435032 1435817 D Similar to AAO89975 (Q83EA3) Pantoate-beta-alanine ligase from Coxiella burnetii (257 aa). FASTA: opt: 692 Z-score: 830.8 E(): 2e-38 Smith-Waterman score: 692; 40.927 identity in 259 aa overlap; pantoate-beta-alanine ligase 1435032..1435817 Francisella tularensis subsp. tularensis SCHU S4 3191378 YP_170336.1 CDS panD NC_006570.2 1435811 1436146 D Converts L-aspartate to beta-alanine and provides the major route of beta-alanine production in bacteria. Beta-alanine is essential for the biosynthesis of pantothenate (vitamin B5); aspartate alpha-decarboxylase 1435811..1436146 Francisella tularensis subsp. tularensis SCHU S4 3191388 YP_170337.1 CDS FTT_1392 NC_006570.2 1436150 1436920 D type III; catalyzes the formation of (R)-4'-phosphopantothenate from (R)-pantothenate in coenzyme A biosynthesis; type III pantothenate kinases are not subject to feedback inhibition from coenzyme A and have a high Km for ATP; pantothenate kinase 1436150..1436920 Francisella tularensis subsp. tularensis SCHU S4 3191493 YP_170338.1 CDS recD NC_006570.2 1436929 1438797 R Similar to Q9RPH5 RecD from Mycobacterium smegmatis (554 aa). FASTA: opt: 593 Z-score: 627.7 E(): 4.1e-27 Smith-Waterman score: 776; 32.510 identity in 486 aa overlap; exodeoxyribonuclease V subunit alpha complement(1436929..1438797) Francisella tularensis subsp. tularensis SCHU S4 3191640 YP_170339.1 CDS recB NC_006570.2 1438763 1442416 R Similar to Q8EF45 Exodeoxyribonuclease V,beta subunit from Shewanella oneidensis (1259 aa). FASTA: opt: 742 Z-score: 770.7 E(): 4.5e-35 Smith-Waterman score: 1544; 30.448identity in 1317 aa overlap; exodeoxyribonuclease V subunit beta complement(1438763..1442416) Francisella tularensis subsp. tularensis SCHU S4 3191642 YP_170340.1 CDS FTT_1395c NC_006570.2 1442488 1443918 R Similar Q8A303 ATP-dependent DNA helicase from Bacteriodes thetaiotamicron (511 aa). FASTA: opt: 496 Z-score: 533.4 E(): 7.4e-22 Smith-Waterman score: 815; 32.200 identity in 500 aa overlap ORF ftt1395c; ATP-dependent DNA helicase complement(1442488..1443918) Francisella tularensis subsp. tularensis SCHU S4 3192384 YP_170341.1 CDS FTT_1396c NC_006570.2 1444061 1444639 R Similar to AAO81663 (Q833N3) Conserved hypothetical protein from Entercoccus faecalis (203 aa). FASTA: opt: 155 Z-score: 198.6 E(): 0.0033 Smith-Waterman score: 155; 25.850 identity in 147 aa overlap ORF ftt1396c; hypothetical protein complement(1444061..1444639) Francisella tularensis subsp. tularensis SCHU S4 3192382 YP_170342.1 CDS recC NC_006570.2 1444575 1447814 R Similar to P44945 Exodeoxyribonuclease V gamma chain from Haemophilus influenzae (1121 aa). FASTA: opt: 1139 Z-score: 1214.3 E(): 8.7e-60 Smith-Waterman score: 1433; 28.344 identity in 1129 aa overlap; exodeoxyribonuclease V subunit gamma complement(1444575..1447814) Francisella tularensis subsp. tularensis SCHU S4 3191641 YP_170343.1 CDS FTT_1399 NC_006570.2 1449653 1450522 D Similar to Q8KH10 Conserved hypothetical integral membrane (299 aa). FASTA: opt: 516 Z-score: 612.8 E(): 2.8e-26 Smith-Waterman score: 516; 29.818 identity in 275 aa overlap ORF ftt1399; hypothetical protein 1449653..1450522 Francisella tularensis subsp. tularensis SCHU S4 3192469 YP_170344.1 CDS FTT_1400c NC_006570.2 1450536 1450922 R ORF ftt1400c; hypothetical protein complement(1450536..1450922) Francisella tularensis subsp. tularensis SCHU S4 3190879 YP_170345.1 CDS FTT_1401 NC_006570.2 1451079 1451744 D Similar to Q8HA35 Repressor (217 aa). FASTA: opt: 183 Z-score: 217.7 E(): 0.00028 Smith-Waterman score: 225; 29.167 identity in 216 aa overlap ORF ftt1401; prophage repressor protein 1451079..1451744 Francisella tularensis subsp. tularensis SCHU S4 3190880 YP_170346.1 CDS FTT_1402c NC_006570.2 1452316 1453950 R ORF ftt1402c; hypothetical protein complement(1452316..1453950) Francisella tularensis subsp. tularensis SCHU S4 3190795 YP_170347.1 CDS feoA NC_006570.2 1453967 1454191 R Similar to P33649 Ferrous iron transport protein A from E. coli (75 aa). FASTA: opt: 148 Z-score: 228.5 E(): 7.1e-05 Smith-Waterman score: 148; 31.081 identity in 74 aa overlap; ferrous iron transport protein A complement(1453967..1454191) Francisella tularensis subsp. tularensis SCHU S4 3191880 YP_170348.1 CDS FTT_1404 NC_006570.2 1454300 1455007 D Similar to Q8E9Y6 Cell division protein FtsN from Shewanella oneidensis (231 aa). FASTA: opt: 157 Z-score: 187.2 E(): 0.014 Smith-Waterman score: 180; 27.094 identity in 203 aa overlap ORF ftt1404; cell division protein 1454300..1455007 Francisella tularensis subsp. tularensis SCHU S4 3190843 YP_170349.1 CDS gloB NC_006570.2 1455085 1455843 R Similar to Q47677 Probable hydroxyacylglutathione hydrolase from E. coli (251 aa). FASTA: opt: 426 Z-score: 519.2 E(): 4.6e-21 Smith-Waterman score: 426; 36.290 identity in 248 aa overlap; hydroxyacylglutathione hydrolase complement(1455085..1455843) Francisella tularensis subsp. tularensis SCHU S4 3192409 YP_170350.1 CDS FTT_1406c NC_006570.2 1455857 1457014 R ORF ftt1406c; hypothetical protein complement(1455857..1457014) Francisella tularensis subsp. tularensis SCHU S4 3190867 YP_170351.1 CDS FTT_1407c NC_006570.2 1457011 1458096 R ORF ftt1407c; hypothetical protein complement(1457011..1458096) Francisella tularensis subsp. tularensis SCHU S4 3192327 YP_170352.1 CDS hemD NC_006570.2 1458062 1458811 R Similar to Q980U3 Uroporphyrinogen III synthase, from Sulfolobus solfataricus (237 aa). FASTA: opt: 171 Z-score: 213.0 E(): 0.00052 Smith-Waterman score: 171; 34.746 identity in 118 aa overlap; uroporphyrinogen III synthase complement(1458062..1458811) Francisella tularensis subsp. tularensis SCHU S4 3192417 YP_170353.1 CDS FTT_1409c NC_006570.2 1458812 1459228 R Similar to Q8PK22 Hypothetical protein from Xanthomonas axonopodis (145 aa). FASTA: opt: 415 Z-score: 523.5 E(): 2.6e-21 Smith-Waterman score: 415; 45.652 identity in 138 aa overlap ORF ftt1409c; hypothetical protein complement(1458812..1459228) Francisella tularensis subsp. tularensis SCHU S4 3192474 YP_170354.1 CDS FTT_1410 NC_006570.2 1459926 1460471 D Similar to Q8XTB4 Probable transmembrane protein from Ralstonia solanacearum (184 aa). FASTA: opt: 443 Z-score: 558.2 E(): 3e-23 Smith-Waterman score: 443; 42.045 identity in 176 aa overlap ORF ftt1410; hypothetical protein 1459926..1460471 Francisella tularensis subsp. tularensis SCHU S4 3192484 YP_170355.1 CDS proS NC_006570.2 1463060 1464757 D catalyzes the formation of prolyl-tRNA(Pro) from proline and tRNA(Pro); prolyl-tRNA synthetase 1463060..1464757 Francisella tularensis subsp. tularensis SCHU S4 3191231 YP_170356.1 CDS FTT_1413 NC_006570.2 1464903 1466033 D Similar to Q886Y5 Aminotransferase, classes I and II from Pseudomonas syringae (382 aa). FASTA: opt: 929 Z-score: 1036.9 E(): 6.6e-50 Smith-Waterman score: 929; 37.076 identity in 383 aa overlap ORF ftt1413; aminotransferase 1464903..1466033 Francisella tularensis subsp. tularensis SCHU S4 3192494 YP_170357.1 CDS FTT_1414 NC_006570.2 1466033 1466515 D ORF ftt1414; hypothetical protein 1466033..1466515 Francisella tularensis subsp. tularensis SCHU S4 3190816 YP_170358.1 CDS FTT_1415 NC_006570.2 1466623 1467525 D ORF ftt1415; hypothetical protein 1466623..1467525 Francisella tularensis subsp. tularensis SCHU S4 3192313 YP_170359.1 CDS FTT_1416c NC_006570.2 1467577 1467990 R ORF ftt1416c; lipoprotein complement(1467577..1467990) Francisella tularensis subsp. tularensis SCHU S4 3191965 YP_170360.1 CDS FTT_1417 NC_006570.2 1468144 1468929 D Similar to Q8ZRJ8 amidohydrolase from Salmonella typhimurium (255 aa). FASTA: opt: 723 Z-score: 882.8 E(): 2.6e-41 Smith-Waterman score: 723; 43.359 identity in 256 aa overlap ORF ftt1417; carbon-nitrogen hydrolase 1468144..1468929 Francisella tularensis subsp. tularensis SCHU S4 3192491 YP_170361.1 CDS nusB NC_006570.2 1468913 1469338 R Similar to Q889Q5 N utilization substance protein B from Pseudomonas syringae (165 aa). FASTA: opt: 274 Z-score: 347.8 E(): 1.6e-11 Smith-Waterman score: 274; 32.847 identity in 137 aa overlap; N utilization substance protein B complement(1468913..1469338) Francisella tularensis subsp. tularensis SCHU S4 3191038 YP_170363.1 CDS FTT_1420 NC_006570.2 1469984 1470451 D ORF ftt1420; hypothetical protein 1469984..1470451 Francisella tularensis subsp. tularensis SCHU S4 3190820 YP_170364.1 CDS FTT_1421 NC_006570.2 1470670 1471119 D Similar to Q88E79 Conserved hypothetical protein from Pseudomonas putida (150 aa). FASTA: opt: 511 Z-score: 638.2 E(): 1.1e-27 Smith-Waterman score: 511; 46.809 identity in 141 aa overlap ORF ftt1421; hypothetical protein 1470670..1471119 Francisella tularensis subsp. tularensis SCHU S4 3190847 YP_170365.1 CDS FTT_1422 NC_006570.2 1471159 1471779 D Similar to Q87UK0 Oxidoreductase, 2OG-Fe(II) oxygenase family (244 aa). FASTA: opt: 400 Z-score: 493.2 E(): 1.3e-19 33.163 identity in 196 aa overlap ORF ftt1422; oxidoreductase 1471159..1471779 Francisella tularensis subsp. tularensis SCHU S4 3191857 YP_170366.1 CDS FTT_1423c NC_006570.2 1471772 1472521 R Similar to Q8NQW1 Predicted permease from Corynebacterium glutamicum (250 aa). FASTA: opt: 472 Z-score: 553.4 E(): 5.6e-23 Smith-Waterman score: 472; 35.573 identity in 253 aa overlap. ORF ftt1423c; hypothetical protein complement(1471772..1472521) Francisella tularensis subsp. tularensis SCHU S4 3192297 YP_170367.1 CDS FTT_1424c NC_006570.2 1472523 1473203 R Similar to Q89VN8 Bll1007 protein from Bradyrhizobium japonicum (345 aa). FASTA: opt: 834 Z-score: 1018.3 E(): 7.2e-49 Smith-Waterman score: 834; 53.509 identity in 228 aa overlap ORF ftt1424c; hypothetical protein complement(1472523..1473203) Francisella tularensis subsp. tularensis SCHU S4 3192493 YP_170368.1 CDS naoX NC_006570.2 1473248 1474942 R Similar to Q8XMF5 NADH oxidase from Clostridium perfringens (566 aa). FASTA: opt: 2055 Z-score: 2177.9 E(): 1.8e-113 57.117 identity in 548 aa overlap; NADH oxidase complement(1473248..1474942) Francisella tularensis subsp. tularensis SCHU S4 3192453 YP_170369.1 CDS FTT_1426c NC_006570.2 1474964 1475482 R Similar to Q8YQC8 Hypothetical protein from Anabaena sp. (177 aa). FASTA: opt: 465 Z-score: 572.2 E(): 5e-24 Smith-Waterman score: 465; 43.023 identity in 172 aa overlap ORF ftt1426c; hypothetical protein complement(1474964..1475482) Francisella tularensis subsp. tularensis SCHU S4 3190849 YP_008492915.1 CDS FTT_1426a NC_006570.2 1475548 1475847 R hypothetical protein complement(1475548..1475847) Francisella tularensis subsp. tularensis SCHU S4 16744730 YP_170370.1 CDS isftu1 NC_006570.2 1476022 1476806 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(1476022..1476348,1476348..1476806) Francisella tularensis subsp. tularensis SCHU S4 3190918 YP_170371.1 CDS FTT_1428c NC_006570.2 1476967 1477482 R Similar to AAP10414 (Q81AS7) Acetyltransferase from Bacillus cereus (174 aa). FASTA: opt: 372 Z-score: 461.6 E(): 7.3e-18 Smith-Waterman score: 372; 40.341 identity in 176 aa overlap ORF ftt1428c; acetyltransferase complement(1476967..1477482) Francisella tularensis subsp. tularensis SCHU S4 3192325 YP_170372.1 CDS rhtC NC_006570.2 1478662 1479297 D Similar Q8DDM9 to threonine efflux protein from Vibrio vulnificus (223 aa). FASTA: opt: 310 Z-score: 386.3 E(): 1.1e-13 Smith-Waterman score: 310; 29.577 identity in 213 aa overlap; threonine efflux protein 1478662..1479297 Francisella tularensis subsp. tularensis SCHU S4 3191627 YP_170373.1 CDS ppnK NC_006570.2 1479294 1480184 R Similar to Q89AR9 inorganic polyphosphate/ATP-NAD kinase from Buchnera aphidicola (292 aa). FASTA: opt: 682 Z-score: 800.3 E(): 1e-36 Smith-Waterman score: 682; 38.776 identity in 294 aa overlap; inorganic phosphate/ATP-NAD kinase complement(1479294..1480184) Francisella tularensis subsp. tularensis SCHU S4 3191987 YP_170374.1 CDS gtrB NC_006570.2 1480373 1481317 D Similar to Q55487 glycosyl transferase from Synechocystis sp. (318 aa). FASTA: opt: 1079 Z-score: 1283.0 E(): 1.3e-63 Smith-Waterman score: 1079; 50.000 identity in 298 aa overlap; glycosyl transferase family protein 1480373..1481317 Francisella tularensis subsp. tularensis SCHU S4 3191777 YP_170375.1 CDS yadH NC_006570.2 1481321 1482100 R Similar Q88LN3 ABC transporter,permease protein from Pseudomonas putida (259 aa). FASTA: opt: 890 Z-score: 979.2 E(): 1.1e-46 Smith-Waterman score: 890; 50.193 identity in 259 aa overlap; ABC transporter permease complement(1481321..1482100) Francisella tularensis subsp. tularensis SCHU S4 3192122 YP_170376.1 CDS yagD NC_006570.2 1482109 1483032 R Similar to Q9CNG2 YagD from Pasteurella multocida (312 aa). FASTA: opt: 1028 Z-score: 1082.3 E(): 2e-52 Smith-Waterman score: 1028; 50.662 identity in 302 aa overlap; ABC transporter ATP-binding protein complement(1482109..1483032) Francisella tularensis subsp. tularensis SCHU S4 3192125 YP_170377.1 CDS isftu2 NC_006570.2 1483215 1483958 D ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 transposase from Francisella tularensis (247 aa).; transposase 1483215..1483958 Francisella tularensis subsp. tularensis SCHU S4 3190848 YP_170378.1 CDS FTT_1439c NC_006570.2 1485237 1486004 R Similar to Q87N28 deoxyribonuclease from vibrio parahaemolyticus (255 aa). FASTA: opt: 787 Z-score: 917.1 E(): 3.1e-43 Smith-Waterman score: 787; 44.314 identity in 255 aa overlap ORF ftt1439c; deoxyribonuclease complement(1485237..1486004) Francisella tularensis subsp. tularensis SCHU S4 3190817 YP_170379.1 CDS FTT_1441 NC_006570.2 1487670 1488155 D Similar to Q8YQ94 Hypothetical protein from Anabaena sp. (144 aa). FASTA: opt: 497 Z-score: 629.3 E(): 3.4e-27 Smith-Waterman score: 497; 54.610 identity in 141 aa overlap ORF ftt1441; hypothetical protein 1487670..1488155 Francisella tularensis subsp. tularensis SCHU S4 3190796 YP_170380.1 CDS rpoA2 NC_006570.2 1488773 1489729 R catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Dimerization of the alpha subunit is the first step in the sequential assembly of subunits to form the holoenzyme; DNA-directed RNA polymerase subunit alpha complement(1488773..1489729) Francisella tularensis subsp. tularensis SCHU S4 3191983 YP_170381.1 CDS FTT_1443c NC_006570.2 1489817 1490719 R Similar to Q87ZS7 ATPase, AAA family from Pseudomonas syringae (440 aa). FASTA: opt: 1018 Z-score: 1104.6 E(): 1.1e-53 Smith-Waterman score: 1096; 43.243 identity in 407 aa overlap ORF ftt1443c; recombination factor protein RarA complement(1489817..1490719) Francisella tularensis subsp. tularensis SCHU S4 3192473 YP_170382.1 CDS ppx NC_006570.2 1491287 1492222 R Similar to P44828 exopolyphosphatase from Pneumophilus influenzae (323 aa). FASTA: opt: 730 Z-score: 856.9 E(): 7.1e-40 Smith-Waterman score: 730; 41.275 identity in 298 aa overlap; exopolyphosphatase complement(1491287..1492222) Francisella tularensis subsp. tularensis SCHU S4 3191242 YP_170383.1 CDS trxA1 NC_006570.2 1492411 1492734 D Similar to Q9JYY9 Thioredoxin from Neisseria meningitidis (110 aa). FASTA: opt: 452 Z-score: 595.9 E(): 2.4e-25 Smith-Waterman score: 452; 62.000 identity in 100 aa overlap; thioredoxin 1492411..1492734 Francisella tularensis subsp. tularensis SCHU S4 3190999 YP_170384.1 CDS rho NC_006570.2 1492777 1494039 D An RNA-DNA helicase that actively releases nascent mRNAs from paused transcription complexes; transcription termination factor Rho 1492777..1494039 Francisella tularensis subsp. tularensis SCHU S4 3191628 YP_170385.1 CDS manB NC_006570.2 1495151 1496635 R Similar to Q8UCV4 Phosphomannomutase from Agrobacterium tumefaciens (506 aa). FASTA: opt: 1226 Z-score: 1345.0 E(): 4.6e-67 Smith-Waterman score: 1226; 41.127 identity in 479 aa overlap; phosphomannomutase complement(1495151..1496635) Francisella tularensis subsp. tularensis SCHU S4 3192273 YP_170386.1 CDS manC NC_006570.2 1496666 1498072 R Similar to Q9ETR3 GDP-mannose pyrophosphorylase from Salmonella enterica (464 aa). FASTA: opt: 1571 Z-score: 1720.2 E(): 5.8e-88 Smith-Waterman score: 1571; 50.647 identity in 464 aa overlap; mannose-1-phosphate guanylyltransferase complement(1496666..1498072) Francisella tularensis subsp. tularensis SCHU S4 3191524 YP_170387.1 CDS wbtM NC_006570.2 1499039 1500085 R Similar to Q9RDY1 RmlB protein from Legionella pneumophila (351 aa). FASTA: opt: 1490 Z-score: 1729.8 E(): 1.7e-88 Smith-Waterman score: 1490; 64.392 identity in 337 aa overlap; dTDP-D-glucose 4,6-dehydratase complement(1499039..1500085) Francisella tularensis subsp. tularensis SCHU S4 3192100 YP_170388.1 CDS wbtL NC_006570.2 1500602 1501486 R Similar to O66250 Glucose-1-phosphate-thymidylyltransferase from Actinobacillus actinomycetemcomitans (290 aa). FASTA: opt: 1336 Z-score: 1567.4 E(): 1.9e-79 Smith-Waterman score: 1336; 67.466 identity in 292 aa overlap; glucose-1-phosphate thymidylyltransferase complement(1500602..1501486) Francisella tularensis subsp. tularensis SCHU S4 3192089 YP_170389.1 CDS wbtK NC_006570.2 1501511 1502371 R Similar to Q8ECG2 Glycosyl transferase, group 2 family protein from Shewanella oneidensis (298 aa). FASTA: opt: 724 Z-score: 845.9 E(): 2.9e-39 Smith-Waterman score: 724; 39.789 identity in 284 aa overlap; glycosyltransferase complement(1501511..1502371) Francisella tularensis subsp. tularensis SCHU S4 3192091 YP_170390.1 CDS wzx NC_006570.2 1502364 1503851 R Similar to AAO39697(Q84DG4) O antigen flippase from E. coli (464 aa). FASTA: opt: 861 Z-score: 954.5 E(): 2.6e-45 Smith-Waterman score: 861; 31.343identity in 469 aa overlap; O-antigen flippase complement(1502364..1503851) Francisella tularensis subsp. tularensis SCHU S4 3192113 YP_170391.1 CDS wbtJ NC_006570.2 1503856 1504581 R Similar to Q50721 Hypothetical protein Rv3404c precurser from Mycobacterium tuberculosis (234 aa). FASTA: opt: 562 Z-score: 656.5 E(): 1e-28 Smith-Waterman score: 562; 43.243 identity in 185 aa overlap; hypothetical protein complement(1503856..1504581) Francisella tularensis subsp. tularensis SCHU S4 3192088 YP_170392.1 CDS wbtI NC_006570.2 1504578 1505660 R Similarity to Q97GL6 Sugar transaminase, involved in dTDP-4-amino-4,6-dideoxyglucose biosynthesis from Clostridium acetobutylicum (364 aa). FASTA: opt: 1344 Z-score: 1521.8 E(): 6.5e-77 Smith-Waterman score: 1344; 59.155 identity in 355 aa overlap; sugar transamine/perosamine synthetase complement(1504578..1505660) Francisella tularensis subsp. tularensis SCHU S4 3192086 YP_170393.1 CDS wbtH NC_006570.2 1505653 1507539 R Similar to Q8GGB6 Asparagine synthase-like protein from Pseudomonas aeruginosa (627 aa). FASTA: opt: 1855 Z-score: 2052.2 E(): 1.9e-106 Smith-Waterman score: 1855; 46.022 identity in 641 aa overlap; asparagine synthase complement(1505653..1507539) Francisella tularensis subsp. tularensis SCHU S4 3192087 YP_170394.1 CDS wbtG NC_006570.2 1507539 1508639 R Similar to Q8ECG6 Glycosyl transferase group 1 family protein from Shewanella oneidensis (373 aa). FASTA: opt: 531 Z-score: 625.5 E(): 5.5e-27 Smith-Waterman score: 531; 32.353 identity in 374 aa overlap; glycosyl transferase family protein complement(1507539..1508639) Francisella tularensis subsp. tularensis SCHU S4 3192084 YP_170395.1 CDS wzy NC_006570.2 1508636 1509865 R membrane protein/O-antigen polymerase (personal communication from authors); membrane protein/O-antigen protein complement(1508636..1509865) Francisella tularensis subsp. tularensis SCHU S4 3192114 YP_170396.1 CDS wbtF NC_006570.2 1509882 1510853 R Similar to Q8KN66 WbpP from Pseudomonas aeruginosa (341 aa). FASTA: opt: 1033 Z-score: 1225.7 E(): 2e-60 Smith-Waterman score: 1033; 51.562identity in 320 aa overlap; NAD dependent epimerase complement(1509882..1510853) Francisella tularensis subsp. tularensis SCHU S4 3192079 YP_170397.1 CDS wbtE NC_006570.2 1510853 1512163 R Similar to Q8EMC3 Vi polysaccharide biosynthesis from Oceanobacillus iheyensis (437 aa). FASTA: opt: 1817 Z-score: 2048.6 E(): 2.9e-106 Smith-Waterman score: 1817; 59.268 identity in 437 aa overlap; UDP-glucose/GDP-mannose dehydrogenase complement(1510853..1512163) Francisella tularensis subsp. tularensis SCHU S4 3192081 YP_170398.1 CDS wbtD NC_006570.2 1512181 1513272 R Similar to Q8EMF7 Galacturonosyl transferase from Oceanobacillus iheyensis (362 aa). FASTA: opt: 908 Z-score: 1050.2 E(): 1.2e-50 Smith-Waterman score: 908; 40.223 identity in 358 aa overlap; galacturonosyl transferase complement(1512181..1513272) Francisella tularensis subsp. tularensis SCHU S4 3192078 YP_170399.1 CDS wbtC NC_006570.2 1513262 1514053 R Similar to Q9K6L4 UDP-glucose 4-epimerase from Bacillus halodurans (282 aa). FASTA: opt: 758 Z-score: 904.3 E(): 1.6e-42 Smith-Waterman score: 758; 45.455 identity in 264 aa overlap; UDP-glucose 4-epimerase complement(1513262..1514053) Francisella tularensis subsp. tularensis SCHU S4 3191944 YP_170400.1 CDS wbtB NC_006570.2 1514055 1514672 R Similarity to Q9K6L3 Galactosyltransferase from Bacillus halodurans (207 aa). FASTA: opt: 902 Z-score: 1122.0 E(): 1.2e-54 Smith-Waterman score: 902; 66.667 identity in 195 aa overlap; galactosyl transferase complement(1514055..1514672) Francisella tularensis subsp. tularensis SCHU S4 3191948 YP_170401.1 CDS wbtA NC_006570.2 1514680 1516416 R Similar to Q9S4V4 PglF from Campylobacter jejuni (590 aa). FASTA: opt: 1343 Z-score: 1435.9 E(): 3.9e-72 Smith-Waterman score: 1378; 43.543identity in 604 aa overlap; dTDP-glucose 4,6-dehydratase complement(1514680..1516416) Francisella tularensis subsp. tularensis SCHU S4 3191939 YP_170402.1 CDS isftu2 NC_006570.2 1516518 1517261 R ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 transposase from Francisella tularensis (247 aa).; transposase complement(1516518..1517261) Francisella tularensis subsp. tularensis SCHU S4 3190875 YP_170403.1 CDS nadB NC_006570.2 1518366 1519853 R Similar to Q8XQG4 L-aspartate oxidase 2 from Ralstonia solanacearum (536 aa). FASTA: opt: 1043 Z-score: 1161.3 E(): 7.8e-57 Smith-Waterman score: 1043; 38.008 identity in 492 aa overlap; L-aspartate oxidase complement(1518366..1519853) Francisella tularensis subsp. tularensis SCHU S4 3192075 YP_170404.1 CDS nadC NC_006570.2 1519856 1520719 R Similar to Q8DC25 Nicotinate-nucleotide pyrophosphorylase from Vibrio vulnificus (295 aa). FASTA: opt: 888 Z-score: 999.5 E(): 8e-48 Smith-Waterman score: 888; 53.737 identity in 281 aa overlap; nicotinate-nucleotide pyrophosphorylase complement(1519856..1520719) Francisella tularensis subsp. tularensis SCHU S4 3190900 YP_170405.1 CDS nadA NC_006570.2 1520728 1521753 R 3 different subfamilies; catalyzes the formation of quinolinate from iminoaspartate and dihydroxyacetone phosphate; quinolinate synthetase complement(1520728..1521753) Francisella tularensis subsp. tularensis SCHU S4 3192072 YP_170406.1 CDS gmk NC_006570.2 1521774 1522346 R Essential for recycling GMP and indirectly, cGMP; guanylate kinase complement(1521774..1522346) Francisella tularensis subsp. tularensis SCHU S4 3192552 YP_170407.1 CDS deaD NC_006570.2 1522463 1524172 R Similar to DEAD_KLEPN (P33906) Cold-shock DEAD-box protein A (ATP-dependent RNA helicase deaD) from Klebsiella pneumoniae (642 aa). FASTA: opt: 1669 Z-score: 1759.9 E(): 3.5e-90 Smith-Waterman score: 1669; 45.070 identity in 568 aa overlap; cold-shock DEAD-box protein A complement(1522463..1524172) Francisella tularensis subsp. tularensis SCHU S4 3191508 YP_170408.1 CDS ppiC NC_006570.2 1524237 1524515 R Identical to Q9ETL6 parvulin ( peptidyl-prolyl cis-trans isomerase) from Francisella tularensis ssp. tularensis strain SchuS4 (FSC237) (92 aa).; peptidyl-prolyl cis-trans isomerase complement(1524237..1524515) Francisella tularensis subsp. tularensis SCHU S4 3192184 YP_170409.1 CDS galP2 NC_006570.2 1524610 1526004 R Similar to GALP__ECOLI Galactose-proton symporter from E. coli (464 aa). FASTA: opt: 965 Z-score: 1103.1 E(): 1.4e-53 Smith-Waterman score: 965; 36.226 identity in 461 aa overlap; major facilitator superfamily galactose-proton symporter complement(1524610..1526004) Francisella tularensis subsp. tularensis SCHU S4 3191585 YP_170410.1 CDS galP1 NC_006570.2 1526036 1527418 R Similar to AAO70555 (Q8XEV6) Galactose-proton symport from Salmonella typhimurium (464 aa). FASTA: opt: 1295 Z-score: 1483.7 E(): 8.6e-75 Smith-Waterman score: 1295; 45.140 identity in 463 aa overlap; major facilitator superfamily galactose-proton symporter complement(1526036..1527418) Francisella tularensis subsp. tularensis SCHU S4 3191546 YP_170411.1 CDS galT NC_006570.2 1527509 1528534 D catalyzes the interconversion of UDP-galactose and galactose-1-P with UDP-galactose and glucose-1-P; galactose-1-phosphate uridylyltransferase 1527509..1528534 Francisella tularensis subsp. tularensis SCHU S4 3192373 YP_170412.1 CDS galK NC_006570.2 1528537 1529685 D Similar to GAL1_ACTPL Galactokinase from Actinobacillus pleuropneumoniae (384 aa) FASTA: opt: 959 Z-score: 1098.0 E(): 2.6e-53 Smith-Waterman score: 959; 38.710identity in 372 aa overlap; galactokinase 1528537..1529685 Francisella tularensis subsp. tularensis SCHU S4 3191539 YP_170413.1 CDS FTT_1477c NC_006570.2 1529682 1530077 R Similar to Q88NK0 Conserved hypothetical protein from Pseudomonas putida (139 aa). FASTA: opt: 362 Z-score: 463.4 E(): 5.9e-18 Smith-Waterman score: 362; 40.458 identity in 131 aa overlap ORF ftt1477c; hypothetical protein complement(1529682..1530077) Francisella tularensis subsp. tularensis SCHU S4 3191765 YP_170414.2 CDS kdsB NC_006570.2 1530070 1530822 R CMP-2-keto-3-deoxyoctulosonic acid synthetase; catalyzes the formation of CMP-3-deoxy-D-manno-octulosonate from CTP and 3-deoxy-D-manno-octulosonate which is incorporated into LPS; 3-deoxy-manno-octulosonate cytidylyltransferase complement(1530070..1530822) Francisella tularensis subsp. tularensis SCHU S4 3192251 YP_170415.1 CDS FTT_1479c NC_006570.2 1530815 1531003 R Similarity to Q8DAV0 Conserved hypothetical protein from Vibrio vulnificus (59 aa). FASTA: opt: 252 Z-score: 369.5 E(): 1e-12 Smith-Waterman score: 252; 56.140 identity in 57 aa overlap ORF ftt1479c; hypothetical protein complement(1530815..1531003) Francisella tularensis subsp. tularensis SCHU S4 3192303 YP_170417.1 CDS isftu1 NC_006570.2 1531728 1532512 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(1531728..1532186,1532186..1532512)) Francisella tularensis subsp. tularensis SCHU S4 3191013 YP_170418.1 CDS lpd NC_006570.2 1532885 1534297 R Similar to AAO90013 (Q83E67) Dihydrolipoamide dehydrogenase from Coxiella burnetti (474 aa). FASTA: opt: 2052 Z-score: 2129.3 E(): 9.5e-111 Smith-Waterman score: 2052; 65.890 identity in 472 aa overlap.; dihydrolipoamide dehydrogenase complement(1532885..1534297) Francisella tularensis subsp. tularensis SCHU S4 3192301 YP_170419.1 CDS aceF NC_006570.2 1534311 1536206 R E2 component of pyruvate dehydrogenase multienzyme complex; in Escherichia coli AceF contains three N-terminal lipoyl domains; dihydrolipoamide acetyltransferase complement(1534311..1536206) Francisella tularensis subsp. tularensis SCHU S4 3192412 YP_170420.1 CDS aceE NC_006570.2 1536224 1538905 R E1 component; part of pyruvate dehydrogenase; forms a complex with DlaT and LpdC; pyruvate dehydrogenase subunit E1 complement(1536224..1538905) Francisella tularensis subsp. tularensis SCHU S4 3192411 YP_170421.1 CDS FTT_1486c NC_006570.2 1539205 1540341 R Similar to AAO91321 (Q83AQ3) Hypothetical protein from Coxiella burnetti (388 aa). FASTA: opt: 858 Z-score: 1007.0 E(): 3.1e-48 Smith-Waterman score: 858 38.504 identity in 361 aa overlap ORF ftt1486c; hypothetical protein complement(1539205..1540341) Francisella tularensis subsp. tularensis SCHU S4 3190829 YP_170422.1 CDS coaE NC_006570.2 1540469 1541083 D catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A; involved in coenzyme A biosynthesis; dephospho-CoA kinase 1540469..1541083 Francisella tularensis subsp. tularensis SCHU S4 3191932 YP_170423.1 CDS trpS NC_006570.2 1541380 1542384 D catalyzes a two-step reaction, first charging a tryptophan molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; tryptophanyl-tRNA synthetase 1541380..1542384 Francisella tularensis subsp. tularensis SCHU S4 3190995 YP_170424.1 CDS FTT_1489 NC_006570.2 1542386 1543111 D ORF ftt1489; hypothetical protein 1542386..1543111 Francisella tularensis subsp. tularensis SCHU S4 3190835 YP_170425.1 CDS FTT_1490 NC_006570.2 1543121 1544419 D Similar to Q9RZK7 NA+/H+ antiporter, , from Deinococcus radiodurans (422 aa). FASTA: opt: 529 Z-score: 612.1 E(): 3.1e-26 Smith-Waterman score: 771; 36.211 identity in 417 aa overlap ORF ftt1490; Na+/H+ antiporter 1543121..1544419 Francisella tularensis subsp. tularensis SCHU S4 3192492 YP_170428.1 CDS FTT_1493c NC_006570.2 1546344 1546739 R ORF ftt1493c; hypothetical protein complement(1546344..1546739) Francisella tularensis subsp. tularensis SCHU S4 3191764 YP_170429.1 CDS murQ NC_006570.2 1546741 1547625 R catalyzes the cleavage of the lactyl ether moiety of N-acetylmuramic acid-6-phosphate (MurNAc-6-P) to form N-acetylglucosamine-6-phosphate (GlcNAc-6-P) and lactate; involved in MurNAc dissimilation pathway; N-acetylmuramic acid 6-phosphate etherase complement(1546741..1547625) Francisella tularensis subsp. tularensis SCHU S4 3192311 YP_170430.1 CDS FTT_1495c NC_006570.2 1547622 1549586 R ORF ftt1495c; hypothetical protein complement(1547622..1549586) Francisella tularensis subsp. tularensis SCHU S4 3192314 YP_170431.1 CDS FTT_1496c NC_006570.2 1549595 1550608 R ORF ftt1496c; hypothetical protein complement(1549595..1550608) Francisella tularensis subsp. tularensis SCHU S4 3190823 YP_170432.1 CDS FTT_1497c NC_006570.2 1550589 1551092 R ORF ftt1497c; hypothetical protein complement(1550589..1551092) Francisella tularensis subsp. tularensis SCHU S4 3192312 YP_170433.1 CDS accA NC_006570.2 1551112 1552059 R catalyzes the carboxylation of acetyl-CoA to malonyl-CoA; forms a tetramer composed of two alpha (AccA) and two beta (AccD) subunits; one of the two catalytic subunits that can form the acetyl CoA carboxylase enzyme together with a carrier protein; acetyl-Coa carboxylase carboxyltransferase subunit alpha complement(1551112..1552059) Francisella tularensis subsp. tularensis SCHU S4 3192200 YP_170434.1 CDS muts NC_006570.2 1552193 1554766 D This protein performs the mismatch recognition step during the DNA repair process; DNA mismatch repair protein MutS 1552193..1554766 Francisella tularensis subsp. tularensis SCHU S4 3192057 YP_170435.1 CDS secB2 NC_006570.2 1554838 1555281 D molecular chaperone that is required for the normal export of envelope proteins out of the cell cytoplasm; in Escherichia coli this proteins forms a homotetramer in the cytoplasm and delivers proteins to be exported to SecA; preprotein translocase subunit SecB 1554838..1555281 Francisella tularensis subsp. tularensis SCHU S4 3191901 YP_170436.1 CDS FTT_1502 NC_006570.2 1556722 1557939 D Similar to AAP17334 (TRYP_ECOLI) Tyrosine-specific transport protein from E. coli (403 aa). FASTA: opt: 741 Z-score: 839.5 E(): 6.5e-39 Smith-Waterman score: 741; 32.663identity in 398 aa overlap ORF ftt1502; aromatic amino acid HAAP transporter 1556722..1557939 Francisella tularensis subsp. tularensis SCHU S4 3190878 YP_170437.1 CDS xerC NC_006570.2 1557949 1558827 D Similar to Q88B11 Integrase/recombinase XerC from Pseudomonas syringae (295 aa). FASTA: opt: 790 Z-score: 939.6 E(): 1.7e-44 Smith-Waterman score: 790; 42.177 identity in 294 aa overlap.; integrase/recombinase XerC 1557949..1558827 Francisella tularensis subsp. tularensis SCHU S4 3191568 YP_170438.1 CDS FTT_1504 NC_006570.2 1558827 1559552 D Similar to YHIQ_NEIGO P72077 Hypothetical 27.3kDa protein from Neisseria gonorrhoeae (254 aa). FASTA: opt: 350 Z-score: 423.8 E(): 9.3e-16 Smith-Waterman score: 350; 36.073 identity in 219 aa overlap. ORF ftt1504; hypothetical protein 1558827..1559552 Francisella tularensis subsp. tularensis SCHU S4 3192496 YP_170439.1 CDS FTT_1505c NC_006570.2 1559559 1560173 R ORF ftt1505c; hypothetical protein complement(1559559..1560173) Francisella tularensis subsp. tularensis SCHU S4 3190866 YP_170440.1 CDS FTT_1506 NC_006570.2 1560261 1560839 D ORF ftt1506; hypothetical protein 1560261..1560839 Francisella tularensis subsp. tularensis SCHU S4 3190870 YP_170441.1 CDS FTT_1507 NC_006570.2 1560916 1561548 D ORF ftt1507; hypothetical protein 1560916..1561548 Francisella tularensis subsp. tularensis SCHU S4 3192309 YP_170442.1 CDS relA NC_006570.2 1561664 1563607 R Similar to Q8EBQ4 GTP pyrophosphokinase from Shewenella oneidensis (735 aa). FASTA: opt: 1566 Z-score: 1723.7 E(): 3.7e-88 Smith-Waterman score: 1639; 39.744 identity in 624 aa overlap; GTP pyrophosphokinase complement(1561664..1563607) Francisella tularensis subsp. tularensis SCHU S4 3191632 YP_170443.1 CDS FTT_1509c NC_006570.2 1563976 1564332 R ORF ftt1509c; hypothetical protein complement(1563976..1564332) Francisella tularensis subsp. tularensis SCHU S4 3190812 YP_170444.1 CDS FTT_1510c NC_006570.2 1564366 1565562 R Similar to Q8DAB5 Tyrosine-specific transport protein from Vibrio vulnificus (401 aa). FASTA: opt: 804 Z-score: 930.6 E(): 5.5e-44 Smith-Waterman score: 804; 35.309 identity in 405 aa overlap ORF ftt1510; aromatic amino acid HAAP transporter complement(1564366..1565562) Francisella tularensis subsp. tularensis SCHU S4 3191763 YP_170445.1 CDS FTT_1511 NC_006570.2 1565753 1566637 D Similar to AAO90839 Membrane protein, from Coxiella burnetii (305 aa). FASTA: opt: 693 Z-score: 824.9 E(): 4.3e-38 Smith-Waterman score: 693; 38.776 identity in 294 aa overlap. ORF ftt1511; hypothetical protein 1565753..1566637 Francisella tularensis subsp. tularensis SCHU S4 3190797 YP_170446.1 CDS dnaJ2 NC_006570.2 1566627 1567553 R Similar to CAD85066 (Q82VD9) DnaJ N-terminal domain:DnaJ C terminal domain from Nitrosomonas europaea (314 aa). FASTA opt: 800 Z-score: 823.9 E(): 4.8e-38 39.482 identity in 309 aa overlap.; heat shock protein, hsp40 complement(1566627..1567553) Francisella tularensis subsp. tularensis SCHU S4 3191530 YP_170447.1 CDS FTT_1514c NC_006570.2 1568483 1568854 R ORF ftt1514c; hypothetical protein complement(1568483..1568854) Francisella tularensis subsp. tularensis SCHU S4 3192319 YP_170448.1 CDS FTT_1515c NC_006570.2 1568862 1569461 R Similar to Q8XMV7 Hypothetical protein CPE0581 from Clostridium perfrigens (195 aa). FASTA: opt: 348 Z-score: 432.5 E(): 3.1e-16 Smith-Waterman score: 348; 33.333 identity in 189 aa overlap. FTT1563 is more likely to be phosphatidylcoline synthase. ORF ftt1515c; hypothetical protein complement(1568862..1569461) Francisella tularensis subsp. tularensis SCHU S4 3192482 YP_170449.1 CDS FTT_1517c NC_006570.2 1570879 1571598 R Similar to Q92KR0 hypothetical transmembrane protein from Rhizobium meliloti (266aa). FASTA: opt: 566 Z-score: 664.8 E(): 3.5e-29 Smith-Waterman score: 566; 40.090 identity in 222 aa overlap. ORF ftt1517c; hypothetical protein complement(1570879..1571598) Francisella tularensis subsp. tularensis SCHU S4 3190872 YP_170450.1 CDS ogt NC_006570.2 1571628 1572200 D Similar to Q8A7W3 methylated-DNA--protein-cysteine methyltransferase from Bacteroides thetaiotaomicron (181aa). FASTA: opt: 443 Z-score: 536.8 bits: 106.7 E(): 4.8e-22 Smith-Waterman score: 443; 44.375 identity in 160 aa overlap. ogt in E.coli is a bifunctional protein containing an autotranscriptional activator at its carboxy domain and a methylated-DNA--protein-cysteine methyltransferase (MGMT) domain at its carboxy terminus. In Bacillus subtilis these two functions are independent and are referred to as adaA and adaB respectively; methylated-DNA--protein-cysteine methyltransferase 1571628..1572200 Francisella tularensis subsp. tularensis SCHU S4 3191050 YP_170451.1 CDS gabP NC_006570.2 1572684 1574021 R Similar to Q8A4M6 glutamate/gamma-aminobutyrate anti-porter from Bacteroides thetaiotaomicron. FASTA: opt: 911 Z-score: 1001.3 E(): 6.4e-48 Smith-Waterman score: 911; 31.696identity in 448 aa overlap.; glutamate/gamma-aminobutyrate anti-porter complement(1572684..1574021) Francisella tularensis subsp. tularensis SCHU S4 3191571 YP_170452.1 CDS FTT_1522c NC_006570.2 1575272 1575862 R Similar to Q8P4A2, hypothetical protein XCC3808 from Xanthamonas campestris (258aa). FASTA: opt:421 Z-score: 521.1 E(): 3.6e-21 Smith-Waterman score: 421; 34.536 identity in 194 aa overlap ORF ftt1522c; hypothetical protein complement(1575272..1575862) Francisella tularensis subsp. tularensis SCHU S4 3191012 YP_170453.1 CDS isftu2 NC_006570.2 1576231 1576974 R ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 transposase from Francisella tularensis (247 aa).; transposase complement(1576231..1576974) Francisella tularensis subsp. tularensis SCHU S4 3192477 YP_170454.1 CDS hrpA NC_006570.2 1577456 1581724 R Similar to Q8ZE61 ATP-dependent helicase from Yersinia pestis (1280 aa). FASTA: opt: 2018 Z-score: 2071.8 E(): 1.5e-107 Smith-Waterman score: 2974; 38.714 identity in 1400 aa overlap; ATp-dependent helicase complement(1577456..1581724) Francisella tularensis subsp. tularensis SCHU S4 3192408 YP_170455.1 CDS FTT_1525c NC_006570.2 1581748 1582635 R ORF ftt1525c; hypothetical protein complement(1581748..1582635) Francisella tularensis subsp. tularensis SCHU S4 3190818 YP_170456.1 CDS idh NC_006570.2 1582649 1584892 R Similar to Q8EDW2 Isocitrate dehydrogenase,NADP-dependent from Shewanella oneidensis (741 aa). FASTA: opt: 3146 Z-score: 3332.9 E(): 8.6e-178 65.574identity in 732 aa overlap; isocitrate dehydrogenase complement(1582649..1584892) Francisella tularensis subsp. tularensis SCHU S4 3192248 YP_170457.1 CDS arsC2 NC_006570.2 1584896 1585261 R Similar to Q87MH6 Arsenate reductase from Vibrio parahaemolyticus (116 aa). FASTA: opt: 287 Z-score: 374.9 E(): 5e-13 Smith-Waterman score: 287; 38.739 identity in 111 aa overlap; arsenate reductase complement(1584896..1585261) Francisella tularensis subsp. tularensis SCHU S4 3191783 YP_170458.1 CDS fadD2 NC_006570.2 1585477 1587165 D Similar to Q8EE09 Long-chain-fatty-acid--CoA ligase from Shewanella oneidensis (557 aa). FASTA: opt: 1556 Z-score: 1747.3 E(): 1.8e-89 Smith-Waterman score: 1556; 42.908identity in 557 aa overlap; long chain fatty acid CoA ligase 1585477..1587165 Francisella tularensis subsp. tularensis SCHU S4 3191875 YP_170459.3 CDS fadE NC_006570.2 1587182 1589443 D functions in fatty acid oxidation; converts acyl-CoA and FAD to FADH2 and delta2-enoyl-CoA; acyl-CoA dehydrogenase 1587182..1589443 Francisella tularensis subsp. tularensis SCHU S4 3191873 YP_170460.1 CDS fadB/acbP NC_006570.2 1589516 1592212 D N-terminal (85 aa) similar to CBP_YEAST (P31787) Acyl-CoA-binding protein (ACBP) from Saccharomyces cerevisiae (86 aa). FASTA opt: 206 Z-score: 281.8 E(): 7.6e-08 Smith-Waterman score: 206; 44.444identity in 72 aa overlap. C-terminus (110-898) similar to Q8P986 3-hydroxacyl-CoA dehydrogenase from Xanthomonas campestris (790 aa). FASTA: opt: 2495 Z-score: 2747.5 E(): 3.5e-145 Smith-Waterman score: 2495;48.228 identity in 790 aa overlap No match to first 110 aa with Q8P986 or any other 3-hydroxacyl-CoA dehydrogenase. Acyl-CoA-binding protein has only been described in eukaryotes unknown EC_number 5.2.2.3; bifunctional 3-hydroxacyl-CoA dehydrogenase/acyl-CoA-binding protein 1589516..1592212 Francisella tularensis subsp. tularensis SCHU S4 3191871 YP_170461.1 CDS fadA NC_006570.2 1592225 1593412 D Catalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation; acetyl-CoA acetyltransferase 1592225..1593412 Francisella tularensis subsp. tularensis SCHU S4 3191866 YP_170462.1 CDS FTT_1532 NC_006570.2 1593474 1593962 D Similar to Q8ABB1 Conserved hypothetical protein from Bacteriodes thetaiotaomicron (163 aa). FASTA: opt: 237 Z-score: 310.7 E(): 1.9e-09 Smith-Waterman score: 237; 27.097 identity in 155 aa overlap ORF ftt1532; hypothetical protein 1593474..1593962 Francisella tularensis subsp. tularensis SCHU S4 3190857 YP_170463.1 CDS FTT_1534c NC_006570.2 1595622 1597037 R Similar to Q8P596 Pre-B cell enhancing factor related protein from Xanthomonas campestris (468 aa). FASTA: opt: 1453 Z-score: 1660.8 E(): 1.2e-84 Smith-Waterman score: 1453; 49.260 identity in 473 aa overlap ORF ftt1534c; nicotinate phosphoribosyltransferase complement(1595622..1597037) Francisella tularensis subsp. tularensis SCHU S4 3190863 YP_170464.1 CDS FTT_1536c NC_006570.2 1598380 1599294 R Similar to Q8RGP8 Hypothetical protein from Mycoplasma penetrans (310 aa). FASTA: opt: 805 Z-score: 956.1 E(): 2.1e-45 Smith-Waterman score: 805; 42.308 identity in 312 aa overlap ORF ftt1536c; hypothetical protein complement(1598380..1599294) Francisella tularensis subsp. tularensis SCHU S4 3192292 YP_170465.1 CDS FTT_1537c NC_006570.2 1599434 1600828 R Similar to Q8DAW7 Conserved hypothetical protein from Vibrio vulnificus (448 aa). FASTA: opt: 455 Z-score: 511.9 E(): 1.2e-20 Smith-Waterman score: 520; 26.539 identity in 471 aa overlap ORF ftt1537c; hypothetical protein complement(1599434..1600828) Francisella tularensis subsp. tularensis SCHU S4 3192317 YP_170466.1 CDS FTT_1538c NC_006570.2 1600834 1601817 R ORF ftt1538c; hypothetical protein complement(1600834..1601817) Francisella tularensis subsp. tularensis SCHU S4 3192498 YP_170467.1 CDS FTT_1539c NC_006570.2 1601828 1603258 R Similar to Q9PJ34 periplasmic protein from Campylobacter jejuni (445 aa). FASTA: opt: 520 Z-score: 539.8 E(): 3.2e-22 Smith-Waterman score: 530; 27.673 identity in 477 aa overlap ORF ftt1539c; hypothetical protein complement(1601828..1603258) Francisella tularensis subsp. tularensis SCHU S4 3190869 YP_170468.1 CDS FTT_1540c NC_006570.2 1603315 1603923 R Similar to Q8EHZ4 Conserved hypothetical protein from Shewanella oneidensis (197 aa). FASTA: opt: 542 Z-score: 654.0 E(): 1.4e-28 Smith-Waterman score: 542; 41.584 identity in 202 aa overlap ORF ftt1540c; hypothetical protein complement(1603315..1603923) Francisella tularensis subsp. tularensis SCHU S4 3192320 YP_170470.1 CDS omp26 NC_006570.2 1604618 1605154 R Similar to Q57483 Outer membrane protein 26 precursor (197 aa). FASTA: opt: 139 Z-score: 159.9 E(): 0.47 Smith-Waterman score: 139; 25.641 identity in 195 aa overlap; hypothetical protein complement(1604618..1605154) Francisella tularensis subsp. tularensis SCHU S4 3191140 YP_170471.1 CDS FTT_1543 NC_006570.2 1605297 1605986 D Similar to Q8UGQ7 Two component response regulator from Agrobacterium tumefaciens (252 aa). FASTA: opt: 684 Z-score: 819.7 E(): 8.4e-38 Smith-Waterman score: 684; 45.333 identity in 225 aa overlap ORF ftt1543; two component response regulator 1605297..1605986 Francisella tularensis subsp. tularensis SCHU S4 3190991 YP_170472.1 CDS isftu1 NC_006570.2 1609794 1610578 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(1609794..1610120,1610120..1610578) Francisella tularensis subsp. tularensis SCHU S4 3190830 YP_170473.1 CDS FTT_1549 NC_006570.2 1611289 1611744 D ORF ftt1549; hypothetical protein 1611289..1611744 Francisella tularensis subsp. tularensis SCHU S4 3192255 YP_170474.1 CDS FTT_1550 NC_006570.2 1611838 1612380 D Similar to Q8EBW9 Conserved hypothetical protein (183 aa). FASTA: opt: 505 Z-score: 618.4 E(): 1.4e-26 Smith-Waterman score: 505; 40.237identity in 169 aa overlap ORF ftt1550; hypothetical protein 1611838..1612380 Francisella tularensis subsp. tularensis SCHU S4 3192230 YP_170475.1 CDS FTT_1551 NC_006570.2 1612382 1613125 D Similar to Q888B1 Oxidoreductase,short chain dehydrogenase from Pseudomonas syringae (262 aa). FASTA: opt: 390 Z-score: 468.5 E(): 3e-18 Smith-Waterman score: 445; 32.143 identity in 252 aa overlap ORF ftt1551; oxidoreductase 1612382..1613125 Francisella tularensis subsp. tularensis SCHU S4 3192231 YP_170476.1 CDS ole1 NC_006570.2 1613139 1613966 D Similar to Q8PMP8 Delta 9 acyl-lipid fatty acid desaturase from Xanthomonas axonopodis (298 aa). FASTA: opt: 851 Z-score: 1027.5 E(): 2.2e-49 Smith-Waterman score: 851; 45.149identity in 268 aa overlap; Delta 9 acyl-lipid fatty acid desaturase 1613139..1613966 Francisella tularensis subsp. tularensis SCHU S4 3191051 YP_170477.1 CDS rnr NC_006570.2 1613990 1616287 R Similar to Q8EAG7 Ribonuclease R from (808 aa). FASTA: opt: 1840 Z-score: 1989.4 E(): 5.9e-103 Smith-Waterman score: 1863; 41.602 identity in 774 aa overlap; ribonuclease R complement(1613990..1616287) Francisella tularensis subsp. tularensis SCHU S4 3191067 YP_170478.1 CDS truB NC_006570.2 1616280 1617188 R catalyzes isomerization of specific uridines in RNA to pseudouridine; responsible for residues in T loops of many tRNAs; tRNA pseudouridine synthase B complement(1616280..1617188) Francisella tularensis subsp. tularensis SCHU S4 3190994 YP_170479.1 CDS rnc NC_006570.2 1617175 1617867 R cytoplasmic enzyme involved in processing rRNA and some mRNAs; substrates typically have dsRNA regions; forms a homodimer; have N-terminal nuclease and C-terminal RNA-binding domains; requires magnesium as preferred ion for activity; ribonuclease III complement(1617175..1617867) Francisella tularensis subsp. tularensis SCHU S4 3191612 YP_170480.1 CDS lepB NC_006570.2 1617874 1618737 R Similar to AAO90612 9 83CL5) Signal peptidase I from Coxiella burnetii (259 aa). FASTA: opt: 716 Z-score: 839.6 E(): 6.5e-39 Smith-Waterman score: 717; 39.416 identity in 274 aa overlap; signal peptidase I complement(1617874..1618737) Francisella tularensis subsp. tularensis SCHU S4 3190721 YP_170481.1 CDS FTT_1557c NC_006570.2 1618742 1619428 R Similar to AAO90736 DNA-binding response regulator from Coxiella burnetii (223 aa). FASTA: opt: 669 Z-score: 771.7 E(): 3.9e-35 Smith-Waterman score: 669; 45.778 identity in 225 aa overlap ORF ftt1557c; two-component response regulator complement(1618742..1619428) Francisella tularensis subsp. tularensis SCHU S4 3190787 YP_170482.1 CDS proC NC_006570.2 1621205 1622029 R Similar to Q87LJ5 Pyrroline-5-carboxylate reductase from Vibrio parahaemolyticus (272 aa). FASTA: opt: 656 Z-score: 770.0 E(): 4.9e-35 Smith-Waterman score: 656; 39.925identity in 268 aa overlap; pyrroline-5-carboxylate reductase complement(1621205..1622029) Francisella tularensis subsp. tularensis SCHU S4 3191232 YP_170483.1 CDS FTT_1560 NC_006570.2 1622136 1622714 D HAM1-like protein; Rec-dependent growth; RgdB; yggV; it is suspected that this protein functions to remove misincorporated bases such as xanthine or hypoxanthine; deoxyribonucleotide triphosphate pyrophosphatase 1622136..1622714 Francisella tularensis subsp. tularensis SCHU S4 3190748 YP_170484.1 CDS kdtA NC_006570.2 1622819 1624114 D Similar to Q87VF9 3-deoxy-D-manno-octulosonic-acid transferase from Pseudomonas syringae (447 aa). FASTA: opt: 1099 Z-score: 1251.3 E(): 7.6e-62 Smith-Waterman score: 1099; 40.143 identity in 421 aa overlap; 3-deoxy-D-manno-octulosonic-acid transferase 1622819..1624114 Francisella tularensis subsp. tularensis SCHU S4 3191532 YP_170485.1 CDS ispA NC_006570.2 1624111 1624992 D Similar to P45204 Geranyltranstransferase from Haemophilus influenzae (295 aa). FASTA: opt: 728 Z-score: 859.4 E(): 5.1e-40 Smith-Waterman score: 728; 43.972 identity in 282 aa overlap; geranyltranstransferase 1624111..1624992 Francisella tularensis subsp. tularensis SCHU S4 3192308 YP_170486.1 CDS pcs NC_006570.2 1625040 1625804 D Similar to O51265 Phosphatidyltransferase from Borrelia burgdorferi (234 aa). FASTA: opt: 438 Z-score: 531.7 E(): 9.1e-22 Smith-Waterman score: 496; 35.743 identity in 249 aa overlap; phosphatidylcholine synthase 1625040..1625804 Francisella tularensis subsp. tularensis SCHU S4 3191290 YP_170487.1 CDS FTT_1564 NC_006570.2 1625874 1626683 D Similar to Q9I6Z1 Hypothetical protein PA0141 from Pseudomonas aeruginosa (298 aa). FASTA: opt: 1210 Z-score: 1427.9 E(): 1.1e-71 Smith-Waterman score: 1210; 62.738 identity in 263 aa overlap ORF ftt1564; hypothetical protein 1625874..1626683 Francisella tularensis subsp. tularensis SCHU S4 3190758 YP_170489.1 CDS FTT_1567c NC_006570.2 1628971 1629558 R ORF ftt1567c; hypothetical protein complement(1628971..1629558) Francisella tularensis subsp. tularensis SCHU S4 3192158 YP_170490.1 CDS lpxB NC_006570.2 1629617 1630759 R Similar to Q87MF0 Lipid-A-disaccharide synthase from Vibrio parahaemolyticus (379 aa). FASTA: 603 opt: 1010 Z-score: 1166.5 E(): 4e-57 Smith-Waterman score: 1010; 41.207 identity in 381 aa overlap; lipid-A-disaccharide synthase complement(1629617..1630759) Francisella tularensis subsp. tularensis SCHU S4 3191793 YP_170491.1 CDS lpxA NC_006570.2 1630759 1631538 R catalyzes the addition of (R)-3-hydroxytetradecanoyl to the glucosamine disaccharide in lipid A biosynthesis; UDP-N-acetylglucosamine acyltransferase complement(1630759..1631538) Francisella tularensis subsp. tularensis SCHU S4 3190814 YP_170492.1 CDS fabZ NC_006570.2 1631541 1632032 R in Pseudomonas aeruginosa this enzyme is a trimer of dimers; essential for membrane formation; performs third step of type II fatty acid biosynthesis; catalyzes dehydration of (3R)-hydroxyacyl-ACP to trans-2-acyl-ACP; (3R)-hydroxymyristoyl-ACP dehydratase complement(1631541..1632032) Francisella tularensis subsp. tularensis SCHU S4 3191868 YP_170493.1 CDS lpxD NC_006570.2 1632047 1633060 R adds the O-linked and N-linked 3(R)-hydroxy fatty acids to the glucosamine disaccharide during lipid A biosynthesis; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase complement(1632047..1633060) Francisella tularensis subsp. tularensis SCHU S4 3190807 YP_170494.1 CDS ompH NC_006570.2 1633079 1633582 R Similar to OMPH_YEREN Cationic 19 kDa outer membrane protein from Yersinia enterocolitica (169 aa). FASTA: opt: 245 Z-score: 251.8 E(): 3.6e-06 Smith-Waterman score: 245; 28.000 identity in 150 aa overlap; outer membrane protein OmpH complement(1633079..1633582) Francisella tularensis subsp. tularensis SCHU S4 3191143 YP_170495.1 CDS FTT_1573c NC_006570.2 1633718 1636096 R Similar to Q88MH2 Outer membrane protein,bacterial surface antigen family from Pseudomonas putida (786 aa). FASTA: opt: 1561 Z-score: 1675.2 E(): 1.9e-85 Smith-Waterman score: 1561; 32.959 identity in 801 aa overlap ORF ftt1573c; hypothetical protein complement(1633718..1636096) Francisella tularensis subsp. tularensis SCHU S4 3192167 YP_170496.1 CDS dxr NC_006570.2 1636102 1637259 R catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate; 1-deoxy-D-xylulose 5-phosphate reductoisomerase complement(1636102..1637259) Francisella tularensis subsp. tularensis SCHU S4 3191554 YP_170497.1 CDS gyrA NC_006570.2 1637265 1639871 R Similar to GYRA_SALTY (P37411) DNA gyrase subunit A from Salmonella typhimurium (878 aa). FASTA: opt: 3442 Z-score: 3640.6 E(): 6.3e-195 Smith-Waterman score: 3442; 60.665 identity in 872 aa overlap; DNA gyrase subunit A complement(1637265..1639871) Francisella tularensis subsp. tularensis SCHU S4 3191786 YP_170498.1 CDS FTT_1576 NC_006570.2 1640130 1640675 D ORF ftt1576; hypothetical protein 1640130..1640675 Francisella tularensis subsp. tularensis SCHU S4 3192137 YP_170499.1 CDS FTT_1577 NC_006570.2 1640711 1641796 D ORF ftt1577; hypothetical protein 1640711..1641796 Francisella tularensis subsp. tularensis SCHU S4 3192144 YP_170500.1 CDS ung NC_006570.2 1641831 1642493 R Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine; uracil-DNA glycosylase complement(1641831..1642493) Francisella tularensis subsp. tularensis SCHU S4 3192316 YP_170501.1 CDS FTT_1579c NC_006570.2 1642490 1645363 R Similar to O25314 Type III restriction enzyme R protein from Helicobacter pylori (1001 aa). FASTA: opt: 1491 Z-score: 1485.7 E(): 6.6e-75 Smith-Waterman score: 2480; 42.828 identity in 990 aa overlap ORF ftt1579c; Type III restriction enzyme complement(1642490..1645363) Francisella tularensis subsp. tularensis SCHU S4 3192096 YP_170502.1 CDS FTT_1580c NC_006570.2 1645600 1646130 R Identical to AAP83333 Hypothetical protein from Francisella tularensis ssp. tularensis strain SchuS4 (FSC237) (176 aa). ORF ftt1580c; hypothetical protein complement(1645600..1646130) Francisella tularensis subsp. tularensis SCHU S4 3192134 YP_170503.1 CDS FTT_1581c NC_006570.2 1646199 1646483 R Similar to Q8DE33 Predicted endonuclease from Vibrio vulnificus (94 aa). FASTA: opt: 387 Z-score: 525.2 E(): 2.1e-21 Smith-Waterman score: 387; 61.798identity in 89 aa overlap ORF ftt1581c; endonuclease complement(1646199..1646483) Francisella tularensis subsp. tularensis SCHU S4 3192076 YP_170504.1 CDS isftu1 NC_006570.2 1648839 1649623 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(1648839..1649297,1649297..1649623)) Francisella tularensis subsp. tularensis SCHU S4 3190919 YP_170506.1 CDS FTT_1586c NC_006570.2 1649883 1650164 R ORF ftt1586c; hypothetical protein complement(1649883..1650164) Francisella tularensis subsp. tularensis SCHU S4 3191930 YP_008492916.1 CDS FTT_1587c NC_006570.2 1650177 1651679 R hypothetical protein complement(1650177..1651679) Francisella tularensis subsp. tularensis SCHU S4 3191942 YP_170507.1 CDS FTT_1589c NC_006570.2 1652045 1652788 R ORF ftt1589c; hypothetical protein complement(1652045..1652788) Francisella tularensis subsp. tularensis SCHU S4 3191908 YP_170508.1 CDS ubiG NC_006570.2 1652791 1653486 R Involved in ubiquinone biosynthesis; 3-demethylubiquinone-9 3-methyltransferase complement(1652791..1653486) Francisella tularensis subsp. tularensis SCHU S4 3192481 YP_170509.1 CDS FTT_1591 NC_006570.2 1653589 1654728 D Similar to Q9Z674 Lipoprotein from Zymomonas mobilis (256 aa). FASTA: opt: 352 Z-score: 404.8 E(): 1.1e-14 Smith-Waterman score: 352; 31.739 identity in 230 aa overlap ORF ftt1591; lipoprotein 1653589..1654728 Francisella tularensis subsp. tularensis SCHU S4 3191537 YP_170510.1 CDS FTT_1594 NC_006570.2 1658651 1659643 D Similar to Q8D7C4 Transcriptional regulator from Vibrio vulnificus (301 aa). FASTA: opt: 364 Z-score: 428.7 E(): 5e-16 Smith-Waterman score: 364; 33.010 identity in 206 aa overlap ORF ftt1594; LysR family transcriptional regulator 1658651..1659643 Francisella tularensis subsp. tularensis SCHU S4 3191502 YP_170513.1 CDS FTT_1597 NC_006570.2 1660677 1662134 D ORF ftt1597; hypothetical protein 1660677..1662134 Francisella tularensis subsp. tularensis SCHU S4 3191501 YP_170514.1 CDS FTT_1598 NC_006570.2 1662198 1665032 D This CDS is disrupted by the insertion of an ISFtu1 element occurring very near the C-terminal end. It is not clear whether this insertion affects the function of the protein. ORF ftt1598; hypothetical protein 1662198..1665032 Francisella tularensis subsp. tularensis SCHU S4 3191599 YP_170515.1 CDS isftu1 NC_006570.2 1665129 1665913 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(1665129..1665587,1665587..1665913)) Francisella tularensis subsp. tularensis SCHU S4 3191801 YP_170516.1 CDS fumA NC_006570.2 1666398 1667912 R Similar to Q87NJ5 Fumarate hydratase, class I from Vibrio parahaemolyticus (505 aa). FASTA: opt: 2398 Z-score: 2635.2 E(): 6.3e-139 Smith-Waterman score: 2398; 69.384 identity in 503 aa overlap; fumerate hydratase complement(1666398..1667912) Francisella tularensis subsp. tularensis SCHU S4 3191534 YP_008492917.1 CDS FTT_1600a NC_006570.2 1668466 1668663 R hypothetical protein complement(1668466..1668663) Francisella tularensis subsp. tularensis SCHU S4 16744732 YP_170517.1 CDS recG NC_006570.2 1668667 1670706 R Similar to Q88C73 ATP-dependent DNA helicase RecG from Pseudomonas putida (692 aa). FASTA: opt: 1698 Z-score: 1867.5 E(): 3.6e-96 Smith-Waterman score: 1698; 41.314 identity in 685 aa overlap; ATP-dependent DNA helicase RecG complement(1668667..1670706) Francisella tularensis subsp. tularensis SCHU S4 3191638 YP_170518.1 CDS FTT_1602 NC_006570.2 1670836 1671180 D ORF ftt1602; hypothetical protein 1670836..1671180 Francisella tularensis subsp. tularensis SCHU S4 3191069 YP_170519.1 CDS rpmB NC_006570.2 1671314 1671550 D required for 70S ribosome assembly; 50S ribosomal protein L28 1671314..1671550 Francisella tularensis subsp. tularensis SCHU S4 3191994 YP_170520.1 CDS rpmG NC_006570.2 1671577 1671732 D in Escherichia coli BM108, a mutation that results in lack of L33 synthesis had no effect on ribosome synthesis or function; there are paralogous genes in several bacterial genomes, and a CXXC motif for zinc binding and an upstream regulation region of the paralog lacking this motif that are regulated by zinc similar to other ribosomal proteins like L31; the proteins in this group lack the CXXC motif; 50S ribosomal protein L33 1671577..1671732 Francisella tularensis subsp. tularensis SCHU S4 3191989 YP_170521.1 CDS minC NC_006570.2 1671767 1672453 D Similar to Q87RC3 Septum site-determining protein MinC from Vibrio parahaemolyticus (220 aa). FASTA: opt: 444 Z-score: 518.9 E(): 4.7e-21 Smith-Waterman score: 444; 39.196 identity in 199 aa overlap; septum site-determining protein MinC 1671767..1672453 Francisella tularensis subsp. tularensis SCHU S4 3192486 YP_170522.1 CDS minD NC_006570.2 1672493 1673317 D Similar to Q8FWX1 Septum site-determining protein MinD from Brucella suis(271 aa). FASTA: opt: 1091 Z-score: 1181.8 E(): 5.6e-58 Smith-Waterman score: 1091; 62.825 identity in 269 aa overlap; septum site-determining protein MinD 1672493..1673317 Francisella tularensis subsp. tularensis SCHU S4 3192434 YP_170523.1 CDS minE NC_006570.2 1673320 1673592 D works in conjunction with MinC and MinD to enable cell division at the midpoint of the long axis of the cell; cell division topological specificity factor MinE 1673320..1673592 Francisella tularensis subsp. tularensis SCHU S4 3191992 YP_170524.1 CDS FTT_1608 NC_006570.2 1673606 1674409 D Similar to YRBF_HAEIN Probable ABC transporter ATP-binding protein HI1087 from Haemophilus influenzae (264 aa). FASTA: opt: 861 Z-score: 970.8 E(): 3.2e-46 Smith-Waterman score: 861; 50.958identity in 261 aa overlap Equivalent to yrbF of yrb operon in E.coli ORF ftt1608; ABC transporter ATP-binding protein 1673606..1674409 Francisella tularensis subsp. tularensis SCHU S4 3191486 YP_170525.1 CDS FTT_1609 NC_006570.2 1674413 1675177 D Similar Q8VUQ9 Toluene resistance protein Trg1 from Pseudomonas putida (265 aa). FASTA: opt: 793 Z-score: 934.5 E(): 3.4e-44 Smith-Waterman score: 793; 55.869 identity in 213 aa overlap Equivalent to yrbE of yrb operon in E.coli ORF ftt1609; ABC transporter permease 1674413..1675177 Francisella tularensis subsp. tularensis SCHU S4 3191579 YP_003097806.1 CDS FTT_1610 NC_006570.2 1675177 1675710 D Similar to YRBD_HAEIN P45029 Hypothetical protein HI1085 from Haemophilus influenzae (167 aa).; hypothetical protein 1675177..1675710 Francisella tularensis subsp. tularensis SCHU S4 8303684 YP_170526.1 CDS FTT_1611 NC_006570.2 1675778 1676446 D Similar to YRBC_ECOLI Protein yrbC precursor from E. coli (211 aa). FASTA: opt: 230 Z-score: 278.3 E(): 1.2e-07 Smith-Waterman score: 230; 26.147 identity in 218 aa overlap Equivalent to yrbC of yrb operon in E.coli ORF ftt1611; hypothetical protein 1675778..1676446 Francisella tularensis subsp. tularensis SCHU S4 3191122 YP_170527.1 CDS FTT_1612 NC_006570.2 1676446 1676739 D Similar to YRBB_ECOLI Hypothetical protein yrbB from E. coli (97 aa). FASTA: opt: 116 Z-score: 165.8 E(): 0.22 Smith-Waterman score: 116; 31.915identity in 47 aa overlap Equivalent to yrbB of yrb operon in E.coli ORF ftt1612; hypothetical protein 1676446..1676739 Francisella tularensis subsp. tularensis SCHU S4 3191129 YP_170528.1 CDS FTT_1613 NC_006570.2 1676756 1676992 D Similar to Q8ZB55 BolA-like protein from Yersinia pestis (87 aa). FASTA: opt: 163 Z-score: 236.9 E(): 2.4e-05 Smith-Waterman score: 163; 35.526 identity in 76 aa overlap Equivalent to yrbA of yrb operon in E.coli ORF ftt1613; BolA-like protein 1676756..1676992 Francisella tularensis subsp. tularensis SCHU S4 3191104 YP_170530.1 CDS isftu1 NC_006570.2 1678189 1678973 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(1678189..1678515,1678515..1678973) Francisella tularensis subsp. tularensis SCHU S4 3192194 YP_170531.1 CDS cysS NC_006570.2 1679445 1680839 D catalyzes a two-step reaction; charges a cysteine by linking its carboxyl group to the alpha-phosphate of ATP then transfers the aminoacyl-adenylate to its tRNA; cysteinyl-tRNA synthetase 1679445..1680839 Francisella tularensis subsp. tularensis SCHU S4 3192385 YP_170532.1 CDS hemK NC_006570.2 1680843 1681787 D involved in methylation of ribosomal protein L3; N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase 1680843..1681787 Francisella tularensis subsp. tularensis SCHU S4 3192421 YP_170533.2 CDS isftu1 NC_006570.2 1683438 1684222 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(1683438..1683764,1683764..1684222) Francisella tularensis subsp. tularensis SCHU S4 3192293 YP_170535.1 CDS FTT_1622c NC_006570.2 1685495 1686415 R ORF ftt1622c; hypothetical protein complement(1685495..1686415) Francisella tularensis subsp. tularensis SCHU S4 3192187 YP_170538.1 CDS FTT_1625c NC_006570.2 1687246 1687836 R ORF ftt1625c; hypothetical protein complement(1687246..1687836) Francisella tularensis subsp. tularensis SCHU S4 3192193 YP_170539.1 CDS FTT_1626c NC_006570.2 1687847 1689253 R ORF ftt1626c; hypothetical protein complement(1687847..1689253) Francisella tularensis subsp. tularensis SCHU S4 3192192 YP_170540.1 CDS FTT_1627c NC_006570.2 1689250 1690053 R ORF ftt1627c; hypothetical protein complement(1689250..1690053) Francisella tularensis subsp. tularensis SCHU S4 3191084 YP_170541.1 CDS FTT_1628c NC_006570.2 1690454 1691764 R Similar to Q88C61 metal ion transporter from Pseudomonas putida (446 aa). FASTA: opt: 1033 Z-score: 1180.6 E(): 6.5e-58 Smith-Waterman score: 1035; 40.455 identity in 440 aa overlap ORF ftt1628c; metal ion transporter complement(1690454..1691764) Francisella tularensis subsp. tularensis SCHU S4 3191086 YP_170542.1 CDS FTT_1629c NC_006570.2 1691775 1693454 R ORF ftt1629c; hypothetical protein complement(1691775..1693454) Francisella tularensis subsp. tularensis SCHU S4 3192479 YP_170543.1 CDS putP NC_006570.2 1693525 1694991 R Similar to PUTP_ECOLI Sodium/proline symporter from E. coli (501 aa). FASTA: opt: 1536 Z-score: 1735.2 E(): 8.4e-89 Smith-Waterman score: 1536; 46.531identity in 490 aa overlap; sodium/proline permease complement(1693525..1694991) Francisella tularensis subsp. tularensis SCHU S4 3191659 YP_170544.1 CDS glpX NC_006570.2 1695297 1696283 R type II fructose 1,6-bisphosphatae; in Escherichia coli this protein forms a dimer and binds manganese; fructose 1,6-bisphosphatase II complement(1695297..1696283) Francisella tularensis subsp. tularensis SCHU S4 3192549 YP_170545.1 CDS FTT_1632c NC_006570.2 1696303 1696941 R Similar to AAO91263 (Q83AV9) Hypothetical protein from Coxiella burnetti (205 aa). FASTA: opt: 552 Z-score: 685.2 E(): 2.6e-30 Smith-Waterman score: 552; 41.000 identity in 200 aa overlap ORF ftt1632c; hypothetical protein complement(1696303..1696941) Francisella tularensis subsp. tularensis SCHU S4 3191085 YP_170546.1 CDS FTT_1633c NC_006570.2 1696950 1698404 R Similiar to AAP09999 (Q81BU4) Lysine-specific permease from Bacillus cereus (491 aa). FASTA: opt: 1734 Z-score: 1972.4 bits: 374.4 E(): 5.2e-102 Smith-Waterman score: 1817; 53.459 identity in 477 aa overlap ORF ftt1633c; amino acid transporter complement(1696950..1698404) Francisella tularensis subsp. tularensis SCHU S4 3191087 YP_170547.1 CDS FTT_1634c NC_006570.2 1698652 1699320 R ORF ftt1634c; hypothetical protein complement(1698652..1699320) Francisella tularensis subsp. tularensis SCHU S4 3192381 YP_170548.1 CDS ftsK NC_006570.2 1699442 1701943 D Similar to Q9I0M3 Cell division protein FtsK from Rickettsia prowazekii (811 aa). FASTA: opt: 2030 Z-score: 2059.0 E(): 7.7e-107 Smith-Waterman score: 2197; 45.808 identity in 823 aa overlap; cell division protein 1699442..1701943 Francisella tularensis subsp. tularensis SCHU S4 3191691 YP_170549.1 CDS lolA NC_006570.2 1701944 1702561 D Similar to LOLA_PSEAE (Q9I0M4) Outer-membrane lipoproteins carrier from Pseudomonas aeruginosa (208 aa). FASTA: opt: 384 Z-score: 440.9 E(): 1e-16 Smith-Waterman score: 384; 30.476 identity in 210 aa overlap; lipoprotein releasing system outer membrane lipoproteins carrier 1701944..1702561 Francisella tularensis subsp. tularensis SCHU S4 3190782 YP_170550.1 CDS FTT_1637c NC_006570.2 1702959 1703162 R ORF ftt1637c; hypothetical protein complement(1702959..1703162) Francisella tularensis subsp. tularensis SCHU S4 3192340 YP_170551.1 CDS trkH NC_006570.2 1703274 1704728 D Similar to Q9KNI3 Potassium uptake protein TrkH from Vibrio cholerae (485 aa). FASTA: opt: 1470 Z-score: 1682.5 E(): 7.3e-86 Smith-Waterman score: 1470; 43.827 identity in 486 aa overlap; potassium uptake protein 1703274..1704728 Francisella tularensis subsp. tularensis SCHU S4 3191471 YP_170552.1 CDS FTT_1639c NC_006570.2 1704743 1705288 R ORF ftt1639c; hypothetical protein complement(1704743..1705288) Francisella tularensis subsp. tularensis SCHU S4 3190827 YP_008492918.1 CDS FTT_1640c NC_006570.2 1705311 1706012 R hypothetical protein complement(1705311..1706012) Francisella tularensis subsp. tularensis SCHU S4 8303709 YP_170553.1 CDS FTT_1644 NC_006570.2 1708832 1710256 D Similar to O86076 Sucrose hydrolase from E. coli (477 aa). FASTA: opt: 787 Z-score: 912.8 E(): 5.4e-43 Smith-Waterman score: 787; 31.929 identity in 451 aa overlap ORF ftt1644; beta-fructofuranosidase 1708832..1710256 Francisella tularensis subsp. tularensis SCHU S4 3192336 YP_170554.1 CDS FTT_1645 NC_006570.2 1710275 1711507 D Similar to Q8EDM5 Conserved hypothetical protein from Shewanella oneidensis (384 aa). FASTA: opt: 372 Z-score: 419.7 E(): 1.6e-15 Smith-Waterman score: 372; 23.018 identity in 391 aa overlap ORF ftt1645; hypothetical protein 1710275..1711507 Francisella tularensis subsp. tularensis SCHU S4 3191089 YP_170556.1 CDS pyrD NC_006570.2 1712449 1713195 R Similar to Q9WYG8 Dihydroorotate dehydrogenase from Pyrobaculum aerophilum (297 aa). FASTA: opt: 148 Z-score: 178.6 E(): 0.043 Smith-Waterman score: 191; 26.568 identity in 271 aa overlap; diyroorotate dehydrogenase complement(1712449..1713195) Francisella tularensis subsp. tularensis SCHU S4 3191654 YP_170557.1 CDS pyrF NC_006570.2 1713197 1713841 R Similar to PYRF_STAAM (Q9LCT0) Orotidine 5'-phosphate decarboxylase from Bradyrhizobium japonicum (237 aa). FASTA: opt: 228 z-score: 280.6 E(): 8.8e-08 Smith-Waterman score: 243; 28.061identity in 196 aa overlap; orotidine 5'-phosphate decarboxylase complement(1713197..1713841) Francisella tularensis subsp. tularensis SCHU S4 3191652 YP_170558.1 CDS FTT_1650c NC_006570.2 1717533 1718081 R Similar to Q93LJ4 Periplasmic monofunctional chorismate mutase from Salmonella typhimurium (181 aa). FASTA: opt: 186 Z-score: 223.0 E(): 0.00014 Smith-Waterman score: 186; 31.746 identity in 126 aa overlap ORF ftt1650; chorismate mutase complement(1717533..1718081) Francisella tularensis subsp. tularensis SCHU S4 3190851 YP_170559.1 CDS FTT_1651 NC_006570.2 1718233 1718859 D Similar to Q8KDD5 Hypothetical protein from Chlorobium tepidum (215 aa). FASTA: opt: 569 z-score: 706.8 E(): 1.6e-31 Smith-Waterman score: 569; 40.580 identity in 207 aa overlap ORF ftt1651; hypothetical protein 1718233..1718859 Francisella tularensis subsp. tularensis SCHU S4 3192480 YP_170560.1 CDS FTT_1652c NC_006570.2 1718860 1719729 R Similar to Q8EFC8 Transcriptional regulator, LysR family from Shewanella oneidensis (291 aa). FASTA: opt: 443 Z-score: 527.9 E(): 1.5e-21 Smith-Waterman score: 443; 29.831 identity in 295 aa overlap ORF ftt1652c; LysR family transcriptional regulator complement(1718860..1719729) Francisella tularensis subsp. tularensis SCHU S4 3190850 YP_170561.1 CDS FTT_1653 NC_006570.2 1719855 1720262 D ORF ftt1653; hypothetical protein 1719855..1720262 Francisella tularensis subsp. tularensis SCHU S4 3190846 YP_170562.1 CDS emrA2 NC_006570.2 1720351 1721316 D Similar to AAO90332 (Q83DE0) Multidrug resistance protein from Coxiella burnetii (331 aa). FASTA: opt: 406 z-score: 447.9 E(): 4.2e-17 Smith-Waterman score: 406; 28.135 identity in 327 aa overlap; HlyD family secretion protein 1720351..1721316 Francisella tularensis subsp. tularensis SCHU S4 3191565 YP_170563.1 CDS FTT_1655 NC_006570.2 1721291 1722355 D ORF ftt1655; hypothetical protein 1721291..1722355 Francisella tularensis subsp. tularensis SCHU S4 3190881 YP_170564.1 CDS FTT_1656c NC_006570.2 1722471 1723022 R Similiar to Q8PGE2 Hypothetical protein from Xanthomonas axonopodis(186 aa). FASTA: opt: 578 Z-score: 703.0 E(): 2.6e-31 Smith-Waterman score: 578; 51.462 identity in 171 aa overlap ORF ftt1656c; hypothetical protein complement(1722471..1723022) Francisella tularensis subsp. tularensis SCHU S4 3190837 YP_170566.1 CDS FTT_1658c NC_006570.2 1723736 1723990 R Similar to BAC68773 (Q82P70) Hypothetical protein from Streptomyces avermitilis (95 aa). FASTA: opt: 135 Z-score: 188.6 E(): 0.012 Smith-Waterman score: 135; 25.316 identity in 79 aa overlap ORF ftt1658c; hypothetical protein complement(1723736..1723990) Francisella tularensis subsp. tularensis SCHU S4 3190815 YP_170567.1 CDS FTT_1659 NC_006570.2 1724243 1725268 D ORF ftt1659; hypothetical protein 1724243..1725268 Francisella tularensis subsp. tularensis SCHU S4 3190833 YP_170568.1 CDS pyrC NC_006570.2 1725296 1726642 R Catalyzes the reversible hydrolysis of the amide bond within dihydroorotate. This metabolic intermediate is required for the biosynthesis of pyrimidine nucleotides; dihydroorotase complement(1725296..1726642) Francisella tularensis subsp. tularensis SCHU S4 3191655 YP_170569.1 CDS tmpT NC_006570.2 1726774 1727454 D catalyzes the S-adenosylmethionine-dependent transmethylation of thiopurine compounds; may be involved in selenium cycling by forming dimethylselenide and/or dimethyldiselenide; thiopurine S-methyltransferase 1726774..1727454 Francisella tularensis subsp. tularensis SCHU S4 3190790 YP_170570.1 CDS carA NC_006570.2 1729048 1730214 D catalyzes production of carbamoyl phosphate from bicarbonate and glutamine in pyrimidine and arginine biosynthesis pathways; forms an octamer composed of four CarAB dimers; carbamoyl phosphate synthase small subunit 1729048..1730214 Francisella tularensis subsp. tularensis SCHU S4 3191074 YP_170571.1 CDS carB NC_006570.2 1730219 1733503 D four CarB-CarA dimers form the carbamoyl phosphate synthetase holoenzyme that catalyzes the production of carbamoyl phosphate; CarB is responsible for the amidotransferase activity; carbamoyl phosphate synthase large subunit 1730219..1733503 Francisella tularensis subsp. tularensis SCHU S4 3191275 YP_170572.1 CDS pyrB NC_006570.2 1733645 1734565 D Similar to PYRB_METJA Aspartate carbamoyltransferase from Methanococcus jannaschii (306 aa). FASTA: opt: 943 Z-score: 1081.5 E(): 2.2e-52 Smith-Waterman score: 943; 49.338 identity in 302 aa overlap; aspartate carbamoyltransferase 1733645..1734565 Francisella tularensis subsp. tularensis SCHU S4 3191656 YP_170573.1 CDS FTT_1666c NC_006570.2 1734578 1735465 R Similar to AAP10961 (Q819E3) 3-hydroxyisobutyrate dehydrogenase from Bacillus cereus (292 aa). FASTA: opt: 384 z-score: 452.2 E(): 2.4e-17 Smith-Waterman score: 384; 24.483 identity in 290 aa overlap ORF ftt1666c; 3-hydroxyisobutyrate dehydrogenase complement(1734578..1735465) Francisella tularensis subsp. tularensis SCHU S4 3192365 YP_170574.1 CDS FTT_1667 NC_006570.2 1735953 1736189 D ORF ftt1667; hypothetical protein 1735953..1736189 Francisella tularensis subsp. tularensis SCHU S4 3191894 YP_170575.1 CDS sdaC2 NC_006570.2 1736516 1737739 D Similar to Q8ZGH6 Serine transporter from Yersinia pestis (433 aa). FASTA: opt: 1336 Z-score: 1510.1 E(): 2.9e-76 Smith-Waterman score: 1336; 46.078identity in 408 aa overlap.; serine transporter 1736516..1737739 Francisella tularensis subsp. tularensis SCHU S4 3191410 YP_170576.1 CDS isftu1 NC_006570.2 1738723 1739507 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(1738723..1739049,1739049..1739507) Francisella tularensis subsp. tularensis SCHU S4 3190921 YP_170577.1 CDS ribD NC_006570.2 1740586 1741653 D Similar to RIBD_AQUAE Riboflavin biosynthesis protein ribD from Aquiflex aeolicus (356 aa). FASTA: opt: 915 z-score: 1060.1 E(): 3.4e-51 Smith-Waterman score: 916; 43.824 identity in 340 aa overlap; riboflavin biosynthesis protein ribD 1740586..1741653 Francisella tularensis subsp. tularensis SCHU S4 3191624 YP_170578.1 CDS ribB NC_006570.2 1741646 1742251 D catalyzes the formation of riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine; riboflavin synthase subunit alpha 1741646..1742251 Francisella tularensis subsp. tularensis SCHU S4 3191625 YP_170579.1 CDS ribA NC_006570.2 1742244 1743455 D Similar to GCH2_AQUAE Riboflavin biosynthesis protein ribA from Aquiflex aeolicus (406 aa). FASTA: opt: 1135 z-score: 1316.5 E(): 1.8e-65 Smith-Waterman score: 1135; 46.305 identity in 406 aa overlap; riboflavin biosynthesis protein ribA/GTP-cyclohydrolase II 1742244..1743455 Francisella tularensis subsp. tularensis SCHU S4 3191626 YP_170580.1 CDS ribH NC_006570.2 1743465 1743908 D Similar to AAO90192 (Q83DP8) Riboflavin synthase, beta subunit from Coxiella burnetii (151 aa). FASTA: opt: 496 z-score: 645.4 E(): 4.2e-28 Smith-Waterman score: 496; 53.571 identity in 140 aa overlap; riboflavin synthase subunit beta 1743465..1743908 Francisella tularensis subsp. tularensis SCHU S4 3191622 YP_170581.1 CDS def2 NC_006570.2 1743905 1744429 D Similar to DEF_NEIMA (Q9JQN0) Peptide deformylase from Neisseria meningitidis (167 aa). FASTA: opt: 465 z-score: 571.7 E(): 5.4e-24 Smith-Waterman score: 465; 46.914 identity in 162 aa overlap; peptide deformylase 1743905..1744429 Francisella tularensis subsp. tularensis SCHU S4 3191511 YP_170582.1 CDS FTT_1676 NC_006570.2 1744511 1745503 D ORF ftt1676; hypothetical protein 1744511..1745503 Francisella tularensis subsp. tularensis SCHU S4 3192368 YP_170583.1 CDS mnmA NC_006570.2 1745523 1746668 R catalyzes a sulfuration reaction to synthesize 2-thiouridine at the U34 position of tRNAs; tRNA-specific 2-thiouridylase MnmA complement(1745523..1746668) Francisella tularensis subsp. tularensis SCHU S4 3191949 YP_170584.1 CDS lepA NC_006570.2 1746616 1748400 R binds to the ribosome on the universally-conserved alpha-sarcin loop; GTP-binding protein LepA complement(1746616..1748400) Francisella tularensis subsp. tularensis SCHU S4 3192146 YP_170585.1 CDS rpsT NC_006570.2 1748670 1748942 D binds directly to the 16S rRNA and is involved in post-translational inhibition of arginine and ornithine decarboxylase; 30S ribosomal protein S20 1748670..1748942 Francisella tularensis subsp. tularensis SCHU S4 3192339 YP_170586.1 CDS olmA NC_006570.2 1749016 1749372 R Similar to Q8KTI1 Outer membrane lipoprotein OmlA from Azaobacter vinelandii (171 aa). FASTA: opt: 208 Z-score: 273.7 E(): 2.1e-07 Smith-Waterman score: 208; 30.392 identity in 102 aa overlap; outer membrane lipoprotein complement(1749016..1749372) Francisella tularensis subsp. tularensis SCHU S4 3191055 YP_170587.1 CDS lpcA NC_006570.2 1749380 1749976 R Similar to Q8EK08 Phosphoheptose isomerase from Shewanella oneidensis (197 aa). FASTA: opt: 705 z-score: 847.8 E(): 2.3e-39 Smith-Waterman score: 705; 53.684 identity in 190 aa overlap; phosphoheptose isomerase complement(1749380..1749976) Francisella tularensis subsp. tularensis SCHU S4 3191473 YP_170588.1 CDS FTT_1683c NC_006570.2 1752525 1753676 R Similar to Q8NM37 Permeases of the major facilitator superfamily from Corynebacterium glutamicum (398 aa). FASTA: opt: 440 z-score: 489.8 E(): 2e-19 Smith-Waterman score: 440; 24.211 identity in 380 aa overlap ORF ftt1683c; major facilitator transporter complement(1752525..1753676) Francisella tularensis subsp. tularensis SCHU S4 3192371 YP_170589.1 CDS FTT_1684 NC_006570.2 1753773 1754675 D Similar to Q8D3N4 Transcriptional regulator from Vibrio vulnificus (307 aa). FASTA: opt: 510 Z-score: 590.3 E(): 5e-25 32.975 identity in 279 aa overlap ORF ftt1684; transcriptional regulator 1753773..1754675 Francisella tularensis subsp. tularensis SCHU S4 3192501 YP_170591.1 CDS FTT_1686c NC_006570.2 1755756 1756424 R Similar to Q894C5 Proline synthetase associated protein from Clostridium tetani (225 aa). FASTA: opt: 462 z-score: 549.8 E(): 8.9e-23 37.220 identity in 223 aa overlap ORF ftt1686c; hypothetical protein complement(1755756..1756424) Francisella tularensis subsp. tularensis SCHU S4 3192358 YP_170592.1 CDS gidB NC_006570.2 1756421 1757038 R glucose-inhibited division protein B; SAM-dependent methyltransferase; methylates the N7 position of guanosine in position 527 of 16S rRNA; 16S rRNA methyltransferase GidB complement(1756421..1757038) Francisella tularensis subsp. tularensis SCHU S4 3192244 YP_170593.1 CDS FTT_1688 NC_006570.2 1757138 1758343 D Similar to Q8EFB8 Tyrosine-specific transport protein from Shewanella oneidensis (395 aa). FASTA: opt: 854 z-score: 967.0 E(): 5.2e-46 38.378 identity in 370 aa overlap ORF ftt1688; aromatic amino acid HAAP transporter 1757138..1758343 Francisella tularensis subsp. tularensis SCHU S4 3192349 YP_170594.1 CDS FTT_1689c NC_006570.2 1758340 1759032 R Similar to CAD84514 (Q82WQ9) Possible transmembrane protein from Nitrosomonas europaea (223 aa). FASTA: opt: 294 z-score: 358.6 E(): 4e-12 25.481 identity in 208 aa overlap ORF ftt1689c; hypothetical protein complement(1758340..1759032) Francisella tularensis subsp. tularensis SCHU S4 3192347 YP_170595.1 CDS FTT_1690 NC_006570.2 1759436 1760101 D Similar to Q9ABA7 NADH dehydrogenase from Caulobacter crescentus (220 aa). FASTA: opt: 429 z-score: 535.0 E(): 6e-22 35.545 identity in 211 aa overlap ORF ftt1690; NADH dehydrogenase 1759436..1760101 Francisella tularensis subsp. tularensis SCHU S4 3192341 YP_170596.1 CDS FTT_1691 NC_006570.2 1760372 1760896 D This CDS is disrupted by the insertion of an ISFtu1 element occurring very near the C-terminal end. It is not clear whether this insertion affects the function of the protein. ORF ftt1691; hypothetical protein 1760372..1760896 Francisella tularensis subsp. tularensis SCHU S4 3192348 YP_170621.1 CDS isftu1 NC_006570.2 1760920 1761704 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(1760920..1761246,1761246..1761704) Francisella tularensis subsp. tularensis SCHU S4 3191802 YP_170598.1 CDS FTT_1693c NC_006570.2 1761847 1762611 R Similar to Q87SC0 conserved hypothetical protein from Vibrio parahaemolyticus (258 aa). FASTA: opt: 724 z-score: 845.1 E(): 3.2e-39 46.094 identity in 256 aa overlap ORF ftt1693c; hypothetical protein complement(1761847..1762611) Francisella tularensis subsp. tularensis SCHU S4 3191497 YP_170599.1 CDS FTT_1694c NC_006570.2 1763252 1763686 R Similar to Q8ZCK6 membrane protein (Hypothetical) from Yersinia pestis (175 aa). FASTA: opt:353 z-score 450 E():2.9e-17 35.9identity in 142 aa overlap ORF ftt1694c; hypothetical protein complement(1763252..1763686) Francisella tularensis subsp. tularensis SCHU S4 3192354 YP_170600.1 CDS groES NC_006570.2 1763937 1764224 D 10 kDa chaperonin; Cpn10; GroES; forms homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring; co-chaperonin GroES 1763937..1764224 Francisella tularensis subsp. tularensis SCHU S4 3191890 YP_170601.1 CDS groEL NC_006570.2 1764262 1765896 D 60 kDa chaperone family; promotes refolding of misfolded polypeptides especially under stressful conditions; forms two stacked rings of heptamers to form a barrel-shaped 14mer; ends can be capped by GroES; misfolded proteins enter the barrel where they are refolded when GroES binds; many bacteria have multiple copies of the groEL gene which are active under different environmental conditions; the B.japonicum protein in this cluster is expressed constitutively; in Rhodobacter, Corynebacterium and Rhizobium this protein is essential for growth; molecular chaperone GroEL 1764262..1765896 Francisella tularensis subsp. tularensis SCHU S4 3191101 YP_170602.2 CDS isftu1 NC_006570.2 1766112 1766896 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(1766112..1766570,1766570..1766896)) Francisella tularensis subsp. tularensis SCHU S4 3190864 YP_170603.1 CDS fdh NC_006570.2 1766954 1767670 R Similar to FDH_PSESR Formate dehydrogenase from Pseudomonas sp. (strain 101) (400 aa). FASTA: opt: 1074 z-score: 1253.7, E(): 5.5e-62, 66.949 identity in 236 aa overlap. This CDS is disrupted by the insertion of an ISFtu1 element occurring very near the C-terminal end. It is not clear whether this insertion affects the function of the protein.; formate dehydrogenase complement(1766954..1767670) Francisella tularensis subsp. tularensis SCHU S4 3191879 YP_170604.1 CDS pdpA2 NC_006570.2 1773260 1775722 D Similar to AAP58968.1 (Q7X3I9) PdpA from Francisella novicida (820 aa). BLAST Score = 1545 bits (4000), Expect = 0.0 Identities = 777/820 (94), Positives = 787/820 (95). Identical to FTT1344. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281; hypothetical protein 1773260..1775722 Francisella tularensis subsp. tularensis SCHU S4 3191298 YP_170605.1 CDS pdpB2 NC_006570.2 1775727 1779008 D Similar to AAP58967.1 (Q7X3J0) PdpB from Francisella novicida (1093 aa). BLAST Score = 1914 bits (4959), Expect = 0.0 Identities = 975/1093 (89),Positives = 988/1093 (90). This protein is identical to FTT1345. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..140828; hypothetical protein 1775727..1779008 Francisella tularensis subsp. tularensis SCHU S4 3191305 YP_170606.1 CDS FTT_1701 NC_006570.2 1778998 1779375 D Identical to FTT1346. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281 ORF ftt1701; hypothetical protein 1778998..1779375 Francisella tularensis subsp. tularensis SCHU S4 3192364 YP_170607.1 CDS FTT_1702 NC_006570.2 1779388 1779882 D Similar to AAP58969.1 (Q7XI38) unknown protein from Francisella novicida (164 aa). BLAST Score = 305 bits (780), Expect = 2e-82 Identities = 163/164 (99),Positives = 163/164 (99). Identical to FTT1347. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281 ORF ftt1702; hypothetical protein 1779388..1779882 Francisella tularensis subsp. tularensis SCHU S4 3192355 YP_170608.1 CDS FTT_1703 NC_006570.2 1779887 1781617 D 1-213 aa similar to AAP58970.1 (Q7X3I7) unknown Francisella novicida (212 aa). BLAST Score 352 ,Expect = 1e-95 Identities = 177/209 (84), Positives = 189/209 (90). 330-580 aa similar to AAP58971.1 (Q7X3I6) unknown protein from Francisella tularensis subsp. novicida (251 aa). BLAST Score = 483 bits (1244), Expect = e-135 Identities = 243/251 (96), Positives = 249/251 (99). Identical to FTT1348. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281. Protein fusion product of two hypothetical proteins in F. novicida. ORF ftt1703; hypothetical protein 1779887..1781617 Francisella tularensis subsp. tularensis SCHU S4 3192367 YP_170609.1 CDS FTT_1704 NC_006570.2 1781618 1782139 D Similar to AAP58972.1 (Q7X3I5) unknown protein from Francisella novicida (173 aa). BLAST Score = 336, Expect = 1e-91 Identities = 170/173 (98), Positives = 171/173 (98). Identical to FTT1349. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281 ORF ftt1704; hypothetical protein 1781618..1782139 Francisella tularensis subsp. tularensis SCHU S4 3192362 YP_170610.1 CDS FTT_1705 NC_006570.2 1782152 1783582 D Similar to AAP58973.1 (Q7X3I4) unknown protein from Francisella novicida (476 aa). BLAST Score = 864 bits (2233), Expect = 0.0 Identities = 433/476 (90),Positives = 437/476 (91). Identical to FTT1350. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281 ORF ftt1705; hypothetical protein 1782152..1783582 Francisella tularensis subsp. tularensis SCHU S4 3192369 YP_170611.1 CDS FTT_1706 NC_006570.2 1783595 1784218 D Similar to AAP58974.1 (Q7X3I3) unknown protein from Francisella novicida (207 aa). BLAST Score = 391 bits (1005), Expect = e-108 Identities = 194/207 (93),Positives = 195/207 (94). Identical to FTT1351. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281 ORF ftt1706; hypothetical protein 1783595..1784218 Francisella tularensis subsp. tularensis SCHU S4 3192554 YP_170612.1 CDS FTT_1707 NC_006570.2 1784240 1785391 D Similar to AAP58975.1 (Q7X3I2) unknown protein from Francisella novicida (262 aa), BLAST Score = 443 bits (1140), Expect = e-12 Identities = 229/262 (87),Positives = 235/262 (89). Identical to FTT1352. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281 ORF ftt1707; hypothetical protein 1784240..1785391 Francisella tularensis subsp. tularensis SCHU S4 3192534 YP_170613.1 CDS FTT_1708 NC_006570.2 1785357 1786130 D Similar to AAP58976.1 (Q7X3I1) unknown protein from Francisella novicida (257 aa). BLAST Score = 412, Expect = e-114 Identities = 213/257 (82), Positives = 213/257 (82). Identical to FTT1353. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281 ORF ftt1708; hypothetical protein 1785357..1786130 Francisella tularensis subsp. tularensis SCHU S4 3192550 YP_170614.1 CDS pdpC2 NC_006570.2 1786170 1790156 D Similar to AAP58966.1 PdpC from Francisella novicida (1218 aa). BLAST Score = 2172, Expect = 0.0 Identities = 1089/1220 (89), Positives = 1120/1220 (91),Gaps = 3/1220 (0). Identical to FTT1354. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281; hypothetical protein 1786170..1790156 Francisella tularensis subsp. tularensis SCHU S4 3192535 YP_170615.1 CDS FTT_1710 NC_006570.2 1790174 1790749 D Similar to AAP58977.1 (Q7X3I0) unknown protein from Francisella novicida (188 aa). BLAST Score = 321, Expect = 3e-87 Identities = 156/174 (89), Positives = 162/174 (93). Identical to FTT1355. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281 ORF ftt1710; hypothetical protein 1790174..1790749 Francisella tularensis subsp. tularensis SCHU S4 3190953 YP_170616.1 CDS iglD2 NC_006570.2 1790756 1791961 R Similar to AAP58965.1 (Q7C3J6) IglD from Francisella novicida (398 aa). BLAST Score = 746, Expect = 0.0 Identities = 382/398 (95), Positives = 383/398 (96) Identical to FTT1356. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281; intracellular growth locus subunit D complement(1790756..1791961) Francisella tularensis subsp. tularensis SCHU S4 3192363 YP_170617.1 CDS iglC2 NC_006570.2 1791973 1792608 R Similar to AAP58964.1 (Q7X3J5) IglC from Francisella novicida (23 kDa protein) (211 aa). BLAST Score = 405,Expect = e-112 Identities = 209/211 (99), Positives = 210/211 (99). Identical to FTT1357. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281; intracellular growth locus subunit C complement(1791973..1792608) Francisella tularensis subsp. tularensis SCHU S4 3191814 YP_170618.1 CDS iglB2 NC_006570.2 1792619 1794163 R Similar to AAP58963.1 (Q7X3J4) IglB from Francisella novicida (514 aa). BLAST Score = 997, Expect = 0.0 Identities = 496/514 (96), Positives = 499/514 (97). Identical to FTT1358. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281; intracellular growth locus subunit B complement(1792619..1794163) Francisella tularensis subsp. tularensis SCHU S4 3191488 YP_170619.1 CDS iglA2 NC_006570.2 1794153 1794743 R Similar to AAP58962.1 (Q7X3J5) IglA from Francisella novicida (184 aa). BLAST Score = 350, Expect = 6e-96 Identities = 181/184 (98), Positives = 181/184 (98). Identical to FTT1359. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281; intracellular growth locus subunit A complement(1794153..1794743) Francisella tularensis subsp. tularensis SCHU S4 3191759 YP_170620.1 CDS pdpD2 NC_006570.2 1794788 1798375 R Similar to AAP58961.1 (Q7C3J6) PdpD from Francisella novicida (1245 aa). BLAST Score = 2194, Expect = 0.0 Identities = 1133/1245 (91), Positives = 1154/1245 (92), Gaps = 50/1245 (4). Identical to FTT1360. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281; hypothetical protein complement(1794788..1798375) Francisella tularensis subsp. tularensis SCHU S4 3191313 YP_170638.1 CDS isftu1 NC_006570.2 1800731 1801515 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(1800731..1801189,1801189..1801515)) Francisella tularensis subsp. tularensis SCHU S4 3190844 YP_170622.1 CDS purL NC_006570.2 1802073 1805945 R catalyzes the formation of 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and L-glutamine in purine biosynthesis; phosphoribosylformylglycinamidine synthase complement(1802073..1805945) Francisella tularensis subsp. tularensis SCHU S4 3191665 YP_170623.1 CDS purF NC_006570.2 1805945 1807435 R Catalyzes first step of the de novo purine nucleotide biosynthetic pathway; amidophosphoribosyltransferase complement(1805945..1807435) Francisella tularensis subsp. tularensis SCHU S4 3191669 YP_170624.1 CDS gad NC_006570.2 1807529 1808875 R Similar to Q8FHG5 Glutamate decarboxylase beta from E. coli (489 aa). FASTA: opt: 1709 Z-score: 1968.5 bits: 373.6 E(): 8.6e-102 Smith-Waterman score: 1709; 57.011 identity in 435 aa overlap; glutamate decarboxylase complement(1807529..1808875) Francisella tularensis subsp. tularensis SCHU S4 3191876 YP_170625.1 CDS FTT_1723c NC_006570.2 1808886 1809530 R Similar to GSP_BACBR (P40683) 4'-phosphopantetheinyl transferase from Bacillus brevis (237 aa). FASTA: opt: 224 Z-score: 292.3 E(): 2.2e-08 Smith-Waterman score: 224; 33.929 identity in 112 aa overlap ORF ftt1723c; hypothetical protein complement(1808886..1809530) Francisella tularensis subsp. tularensis SCHU S4 3192138 YP_170626.1 CDS tolC NC_006570.2 1809536 1811065 R Similar to TOLC_ECOLI (P02930) Outer membrane protein tolC precursor from E. coli (495 aa). FASTA: opt: 548 Z-score: 649.7 E(): 2.7e-28 Smith-Waterman score: 548; 28.000 identity in 425 aa overlap; outer membrane protein TolC complement(1809536..1811065) Francisella tularensis subsp. tularensis SCHU S4 3191456 YP_170627.1 CDS pcm NC_006570.2 1811074 1811694 R Similar to Q9JXU0 Protein-L-isoaspartate O-methyltransferase from Neisseria meningitidis (218 aa). FASTA: opt: 335 Z-score: 438.9 E(): 1.5e-16 Smith-Waterman score: 335; 33.659 identity in 205 aa overlap; protein-L-isoaspartate O-methyltransferase complement(1811074..1811694) Francisella tularensis subsp. tularensis SCHU S4 3191449 YP_170628.1 CDS yegQ NC_006570.2 1812014 1813345 D Similar to YEGQ_ECOLI (P76403) protease yegQ from E. coli (453 aa). FASTA: opt: 1828 Z-score: 2215.7 E(): 1.6e-115 Smith-Waterman score: 1828; 61.745identity in 447 aa overlap Truncated ortholog (188 aa) previously reported in Schu as O68834 Hypothetical 21.3 kDa protein and AAC12939 unknown; protease yegQ 1812014..1813345 Francisella tularensis subsp. tularensis SCHU S4 3192162 YP_170629.1 CDS FTT_1727c NC_006570.2 1813386 1814576 R Similar to Q83CE1 Drug resistance transporter, Bcr/CflA family from Coxiella burnetii (398 aa). FASTA: opt: 957 Z-score: 1006.0 E(): 3.8e-48 Smith-Waterman score: 957; 38.542identity in 384 aa overlap ORF ftt1727c; multidrug resistance protein complement(1813386..1814576) Francisella tularensis subsp. tularensis SCHU S4 3192514 YP_170630.1 CDS nhaD NC_006570.2 1814742 1816169 D Similar to Q8D4S6 Na+/H+ antiporter NhaD from Vibrio vulnificus (478 aa). FASTA: opt: 1428 Z-score: 1679.2 E(): 1.2e-85 Smith-Waterman score: 1592; 50.617identity in 486 aa overlap; Na+/H+ antiporter 1814742..1816169 Francisella tularensis subsp. tularensis SCHU S4 3191701 YP_170631.1 CDS FTT_1730c NC_006570.2 1817820 1819694 R Similar to Q97MC6 Amino acid transporter from Clostridium acetobutylicum (620 aa). FASTA: opt: 1338 Z-score: 1514.2 E(): 1.9e-76 Smith-Waterman score: 1338; 37.745 identity in 612 aa overlap ORF ftt1730c; amino acid transporter complement(1817820..1819694) Francisella tularensis subsp. tularensis SCHU S4 3192505 YP_170632.1 CDS obgE NC_006570.2 1819701 1820705 R essential GTPase; exhibits high exchange rate for GTP/GDP; associates with 50S ribosomal subunit; involved in regulation of chromosomal replication; GTPase ObgE complement(1819701..1820705) Francisella tularensis subsp. tularensis SCHU S4 3191931 YP_170633.1 CDS FTT_1732c NC_006570.2 1820781 1821977 R Similar to Q8FGM8 Tyrosine-specific transport protein from E. coli (403 aa). FASTA: opt: 724 Z-score: 839.2 E(): 6.8e-39 Smith-Waterman score: 724; 35.638 identity in 376 aa overlap ORF ftt1732; aromatic amino acid HAAP transporter complement(1820781..1821977) Francisella tularensis subsp. tularensis SCHU S4 3192136 YP_170635.1 CDS kdpD NC_006570.2 1823996 1826677 R Similar to Q8U9E1 Two component sensor kinase from Agrobacterium tumefaciens (900 aa). FASTA: opt: 1625 Z-score: 1777.7 E(): 3.6e-91 Smith-Waterman score: 1625; 32.745 identity in 907 aa overlap; two component sensor protein KdpD complement(1823996..1826677) Francisella tularensis subsp. tularensis SCHU S4 3192246 YP_170636.1 CDS kdpC NC_006570.2 1826781 1827335 R Similar to Q8R8I7 K+-transporting ATPase, c chain from Thermoanaerobacter tengcogenesis (193 aa). FASTA: opt: 404 Z-score: 471.4 E(): 2.1e-18 Smith-Waterman score: 404; 43.333identity in 180 aa overlap; potassium-transporting ATPase subunit C complement(1826781..1827335) Francisella tularensis subsp. tularensis SCHU S4 3192156 YP_170637.1 CDS kdpB NC_006570.2 1827352 1829391 R Similar to ATKB_RHIME (Q92XJ0) Potassium-transporting ATPase B chain from Rhizobium meliloti (680 aa). FASTA: opt: 2393 Z-score: 2638.7 E(): 4.4e-139 Smith-Waterman score: 2393; 55.490 identity in 683 aa overlap; potassium-transporting ATPase subunit B complement(1827352..1829391) Francisella tularensis subsp. tularensis SCHU S4 3192083 YP_170649.1 CDS isftu1 NC_006570.2 1831850 1832634 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(1831850..1832308,1832308..1832634)) Francisella tularensis subsp. tularensis SCHU S4 3190922 YP_170639.1 CDS ipdC NC_006570.2 1833371 1835068 R Similar to Q8TT45 Indolepyruvate decarboxylase Methanosarcina activorans (550 aa). FASTA: opt: 1265 Z-score: 1467.5 E(): 7.6e-74 Smith-Waterman score: 1265; 36.909 identity in 550 aa overlap; indolepyruvate decarboxylase complement(1833371..1835068) Francisella tularensis subsp. tularensis SCHU S4 3191884 YP_170640.1 CDS FTT_1746 NC_006570.2 1835599 1836522 D Similar to Q87YG1 Signal peptide peptidase SppA, 36K type from Pseudomonas syringae (332 a). FASTA: opt: 839 Z-score: 1007.2 E(): 3.3e-48 Smith-Waterman score: 839; 46.622 identity in 296 aa overlap ORF ftt1746; peptidase 1835599..1836522 Francisella tularensis subsp. tularensis SCHU S4 3192539 YP_170641.1 CDS FTT_1747 NC_006570.2 1836576 1837151 D Similar to AAO90156 (Q83DT1) Outer membrane protein OmpH, , from Coxiella burnetii (165 aa). FASTA: opt: 175 Z-score: 183.6 E(): 0.022 Smith-Waterman score: 216; 26.816 identity in 179 aa overlap ORF ftt1747; hypothetical protein 1836576..1837151 Francisella tularensis subsp. tularensis SCHU S4 3191766 YP_170642.1 CDS glpe NC_006570.2 1837287 1837709 D Similar to AAO91018 (Q83BI7) Rhodanese domain protein Coxiella burnetii (144 aa). FASTA: opt: 279 Z-score: 342.5 E(): 3.5e-11 Smith-Waterman score: 279; 33.094 identity in 139 aa overlap; thiosulfate sulfurtransferase 1837287..1837709 Francisella tularensis subsp. tularensis SCHU S4 3190942 YP_170643.1 CDS secB1 NC_006570.2 1837736 1838185 D molecular chaperone that is required for the normal export of envelope proteins out of the cell cytoplasm; in Escherichia coli this proteins forms a homotetramer in the cytoplasm and delivers proteins to be exported to SecA; preprotein translocase subunit SecB 1837736..1838185 Francisella tularensis subsp. tularensis SCHU S4 3191208 YP_170644.1 CDS recA NC_006570.2 1838207 1839286 D catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs; recombinase A 1838207..1839286 Francisella tularensis subsp. tularensis SCHU S4 3191643 YP_170645.1 CDS recX NC_006570.2 1839273 1839725 D Similar to RECX_HAEIN (P43706) Regulatory protein recX from Haemophilus influenzae (126 aa). FASTA: opt: 243 Z-score: 314.3 E(): 1.3e-09 Smith-Waterman score: 243; 31.967 identity in 122 aa overlap; regulatory protein recX 1839273..1839725 Francisella tularensis subsp. tularensis SCHU S4 3191633 YP_170646.1 CDS ssb NC_006570.2 1839898 1840374 D Similar to SSB_HAEIN P44409 Single-strand binding protein (SSB) from Haemophilus influenzae (168 aa). FASTA: opt: 437 Z-score: 543.8 E(): 1.9e-22 Smith-Waterman score: 437; 44.578identity in 166 aa overlap; single-strand binding protein 1839898..1840374 Francisella tularensis subsp. tularensis SCHU S4 3190727 YP_170647.1 CDS tdcD NC_006570.2 1840480 1841634 D Similar to ACKA_CLOTS (Q59331) Acetate kinase (Acetokinase) from Clostridium thermosaccharolyticum (400 aa). FASTA: opt: 1204 Z-score: 1394.5 E(): 8e-70 Smith-Waterman score: 1204; 49.873 identity in 395 aa overlap; propionate kinase 1840480..1841634 Francisella tularensis subsp. tularensis SCHU S4 3190747 YP_170648.1 CDS pta NC_006570.2 1841644 1843740 D catalyzes the synthesis of acetylphosphate or propionylphosphate from acetyl-CoA or propionyl-CoA and inorganic phosphate; when using propionyl-CoA the enzyme is functioning in the anaerobic pathway catabolizing threonine to propionate; phosphate acetyltransferase 1841644..1843740 Francisella tularensis subsp. tularensis SCHU S4 3191229 YP_170668.1 CDS isftu1 NC_006570.2 1844748 1845532 R ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase complement(join(1844748..1845206,1845206..1845532)) Francisella tularensis subsp. tularensis SCHU S4 3191804 YP_170650.1 CDS FTT_1758c NC_006570.2 1846969 1847562 R Similar to Q8D0W2 cytochrome from Yersinia pestis (199 aa). FASTA: opt: 273 Z-score: 328.7 E(): 1.9e-10 Smith-Waterman score: 273; 27.979identity in 193 aa overlap ORF ftt1758c; B-type cytochrome complement(1846969..1847562) Francisella tularensis subsp. tularensis SCHU S4 3190935 YP_170651.1 CDS nhaA NC_006570.2 1848530 1849681 D exports sodium by using the electrochemical proton gradient to allow protons into the cell; functions in adaptation to high salinity and alkaline pH; activity increases at higher pH; downregulated at acidic pH; pH-dependent sodium/proton antiporter 1848530..1849681 Francisella tularensis subsp. tularensis SCHU S4 3191689 YP_170652.1 CDS FTT_1761 NC_006570.2 1849746 1850465 D Similar to YAFD_ECOLI (P30865) Hypothetical protein yafD from E. coli (266 aa). FASTA: opt: 320 Z-score: 403.2 E(): 1.5e-14 Smith-Waterman score: 320; 34.498 identity in 229 aa overlap ORF ftt1761; hypothetical protein 1849746..1850465 Francisella tularensis subsp. tularensis SCHU S4 3191889 YP_170653.1 CDS FTT_1762c NC_006570.2 1850471 1851211 R Similar to Q9F728 N-acylhomoserine lactone synthase from Pseudomonas fluorescens (259 aa). FASTA: opt: 408 Z-score: 521.4 E(): 3.8e-21 Smith-Waterman score: 408; 34.498 identity in 229 aa overlap ORF ftt1762c; acetyltransferase complement(1850471..1851211) Francisella tularensis subsp. tularensis SCHU S4 3190934 YP_170654.1 CDS FTT_1763c NC_006570.2 1851225 1851977 R Similar to Q9F728 N-acylhomoserine lactone syntase from Pseudomonas fluorescens (259 aa). FASTA: opt: 442 Z-score: 546.9 E(): 1.4e-22 Smith-Waterman score: 442; 37.963 identity in 216 aa overlap. ORF ftt1763c; acetyltransferase complement(1851225..1851977) Francisella tularensis subsp. tularensis SCHU S4 3190865 YP_170655.1 CDS FTT_1764c NC_006570.2 1852140 1852463 R Similar to FER_PSEST (P08811) Ferredoxin from Pseudomonas stutzeri (106 aa). FASTA: opt: 535 Z-score: 674.6 E(): 1.1e-29 Smith-Waterman score: 535; 65.714identity in 105 aa overlap ORF ftt1764c; ferredoxin complement(1852140..1852463) Francisella tularensis subsp. tularensis SCHU S4 3190855 YP_170656.1 CDS FTT_1765 NC_006570.2 1852683 1853336 D ORF ftt1765; hypothetical protein 1852683..1853336 Francisella tularensis subsp. tularensis SCHU S4 3190854 YP_170657.1 CDS FTT_1766 NC_006570.2 1853414 1854070 D Similar to Q8YLW7 O-methyltransferase from Anabaena sp. (220 aa). FASTA: opt: 622 Z-score: 791.6 E(): 3.4e-36 Smith-Waterman score: 622; 43.836identity in 219 aa overlap ORF ftt1766; O-methyltransferase 1853414..1854070 Francisella tularensis subsp. tularensis SCHU S4 3190939 YP_170658.1 CDS purT NC_006570.2 1854072 1855232 R non-folate utilizing enzyme, catalyzes the production of beta-formyl glycinamide ribonucleotide from formate, ATP, and beta-GAR and a side reaction producing acetyl phosphate and ADP from acetate and ATP; involved in de novo purine biosynthesis; phosphoribosylglycinamide formyltransferase 2 complement(1854072..1855232) Francisella tularensis subsp. tularensis SCHU S4 3191662 YP_170659.1 CDS FTT_1768c NC_006570.2 1855286 1857106 R Similar to Q9KQP6 Chitinase from Vibrio cholerae (846 aa). FASTA: opt: 242 Z-score: 272.3 E(): 2.8e-07 Smith-Waterman score: 279; 21.309 identity in 718 aa overlap. ORF ftt1768c; chitinase complement(1855286..1857106) Francisella tularensis subsp. tularensis SCHU S4 3190860 YP_170660.1 CDS clpB NC_006570.2 1857728 1860307 R Similar to FASTA: AAO89660 (Q83F55) ClpB protein from Coxiella burnetii (859 aa). FASTA: opt: 3593 Z-score: 3223.6 E(): 1.1e-171 Smith-Waterman score: 3593; 65.357 identity in 840 aa overlap; ClpB protein complement(1857728..1860307) Francisella tularensis subsp. tularensis SCHU S4 3192509 YP_170661.1 CDS FTT_1771 NC_006570.2 1861326 1861679 D ORF ftt1771; hypothetical protein 1861326..1861679 Francisella tularensis subsp. tularensis SCHU S4 3192304 YP_170662.1 CDS trpA NC_006570.2 1861734 1862543 R catalyzes the formation of indole and glyceraldehyde 3-phosphate from indoleglycerol phosphate in tryptophan biosynthesis; tryptophan synthase subunit alpha complement(1861734..1862543) Francisella tularensis subsp. tularensis SCHU S4 3190980 YP_170663.1 CDS trpB NC_006570.2 1862543 1863733 R catalyzes the formation of L-tryptophan from L-serine and 1-(indol-3-yl)glycerol 3-phosphate; tryptophan synthase subunit beta complement(1862543..1863733) Francisella tularensis subsp. tularensis SCHU S4 3190984 YP_170664.1 CDS clcA NC_006570.2 1865149 1866678 R Similar to CLCA_ECOLI (P37019) Voltage-gated ClC-type chloride channel from E. coli (473 aa). FASTA: opt: 1120 Z-score: 1211.7 E(): 1.3e-59 Smith-Waterman score: 1120; 39.545 identity in 440 aa overlap; voltage-gated ClC-type chloride channel clcA complement(1865149..1866678) Francisella tularensis subsp. tularensis SCHU S4 3191542 YP_170665.1 CDS FTT_1776c NC_006570.2 1866749 1867147 R ORF ftt1776c; hypothetical protein complement(1866749..1867147) Francisella tularensis subsp. tularensis SCHU S4 3190853 YP_170666.1 CDS FTT_1777c NC_006570.2 1867150 1867560 R ORF ftt1777c; hypothetical protein complement(1867150..1867560) Francisella tularensis subsp. tularensis SCHU S4 3192294 YP_170667.1 CDS FTT_1778c NC_006570.2 1867581 1867973 R ORF ftt1778c; hypothetical protein complement(1867581..1867973) Francisella tularensis subsp. tularensis SCHU S4 3190801 YP_003097807.1 CDS isftu1 NC_006570.2 1868806 1869590 D ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase join(1868806..1869132,1869132..1869590) Francisella tularensis subsp. tularensis SCHU S4 3190923 YP_170669.1 CDS FTT_1781c NC_006570.2 1869829 1870578 R ORF ftt1781c; hypothetical protein complement(1869829..1870578) Francisella tularensis subsp. tularensis SCHU S4 3190804 YP_170670.1 CDS yjjK NC_006570.2 1870520 1872199 R ChvD; in Agrobacterium tumefaciens, mutations in both Walker boxes were found to affect virulence; ABC transporter ATP-binding protein complement(1870520..1872199) Francisella tularensis subsp. tularensis SCHU S4 3192393 YP_170671.1 CDS FTT_1783 NC_006570.2 1872367 1873653 D Similar to Q83A52 Major facilitator family transporter from Coxiella burnetii (443 aa). FASTA: opt: 751 Z-score: 809.6 E(): 3.3e-37 Smith-Waterman score: 751; 34.204identity in 421 aa overlap ORF ftt1783; major facilitator transporter 1872367..1873653 Francisella tularensis subsp. tularensis SCHU S4 3190882 YP_170672.1 CDS FTT_1784c NC_006570.2 1873634 1873942 R ORF ftt1784c; hypothetical protein complement(1873634..1873942) Francisella tularensis subsp. tularensis SCHU S4 3191772 YP_170673.1 CDS FTT_1785c NC_006570.2 1873954 1874166 R ORF ftt1785c; hypothetical protein complement(1873954..1874166) Francisella tularensis subsp. tularensis SCHU S4 3191779 YP_170674.1 CDS FTT_1787c NC_006570.2 1875200 1875811 R Similar to Q8UGV8 LysE family transporter from Agrobactium tumifaciens (202 aa). FASTA: opt: 346 Z-score: 414.9 E(): 3.2e-15 Smith-Waterman score: 346; 30.102 identity in 196 aa overlap ORF ftt1787c; amino acid transporter LysE complement(1875200..1875811) Francisella tularensis subsp. tularensis SCHU S4 3191282 YP_170675.1 CDS FTT_1789 NC_006570.2 1877112 1877906 D Similar to Q83C04 Membrane protein, , from Coxiella burnetii (263 aa). FASTA: opt: 496 Z-score: 540.4 E(): 3.3e-22 Smith-Waterman score: 496; 30.038 identity in 263 aa overlap ORF ftt1789; hypothetical protein 1877112..1877906 Francisella tularensis subsp. tularensis SCHU S4 3191776 YP_170676.1 CDS FTT_1791 NC_006570.2 1878315 1878677 D ORF ftt1791; hypothetical protein 1878315..1878677 Francisella tularensis subsp. tularensis SCHU S4 3191780 YP_170677.1 CDS pepN NC_006570.2 1879809 1882403 R Similar to AMPN_ECOLI (P04825) Aminopeptidase N from E. coli (869 aa). FASTA: opt: 2226 Z-score: 2532.9 E(): 3.4e-133 Smith-Waterman score: 2751; 48.856identity in 874 aa overlap; aminopeptidase complement(1879809..1882403) Francisella tularensis subsp. tularensis SCHU S4 3191327 YP_170678.1 CDS FTT_1794 NC_006570.2 1882587 1883015 D Similar to Q9WYK7 Heat shock protein, class I from Thermatoga maritima (147 aa). FASTA: opt: 288 Z-score: 346.4 E(): 2.1e-11 Smith-Waterman score: 288; 37.879 identity in 132 aa overlap ORF ftt1794; heat shock protein 1882587..1883015 Francisella tularensis subsp. tularensis SCHU S4 3192536 YP_170679.1 CDS trpC NC_006570.2 1883012 1884373 R monomeric bifunctional protein; functions in tryptophan biosynthesis pathway; phosphoribosylanthranilate is rearranged to carboxyphenylaminodeoxyribulosephosphate which is then closed to form indole-3-glycerol phosphate; bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase complement(1883012..1884373) Francisella tularensis subsp. tularensis SCHU S4 3190977 YP_170680.1 CDS FTT_1796c NC_006570.2 1884464 1884877 R Similar to Q9KF27 Hypothetical protein BH0670 from Bacillus halodurans (159 aa). FASTA: opt: 208 Z-score: 278.0 E(): 1.4e-07 Smith-Waterman score: 208; 28.682 identity in 129 aa overlap ORF ftt1796c; hypothetical protein complement(1884464..1884877) Francisella tularensis subsp. tularensis SCHU S4 3191782 YP_170681.1 CDS msrA2 NC_006570.2 1884882 1885571 R Similar to MSA2_CAUCR (Q9A9E9) Peptide methionine sulfoxide reductase from Caulobacter crescentus (196 aa). FASTA opt: 510 Z-score: 598.2 E(): 2e-25 Smith-Waterman score: 510; 39.024 identity in 205 aa overlap; peptide methionine sulfoxide reductase complement(1884882..1885571) Francisella tularensis subsp. tularensis SCHU S4 3191678 YP_170682.1 CDS FTT_1798c NC_006570.2 1885655 1886023 R ORF ftt1798c; hypothetical protein complement(1885655..1886023) Francisella tularensis subsp. tularensis SCHU S4 3192283 YP_170683.1 CDS FTT_1800c NC_006570.2 1886426 1886878 R ORF ftt1800c; hypothetical protein complement(1886426..1886878) Francisella tularensis subsp. tularensis SCHU S4 3191269 YP_008492919.1 CDS FTT_1801c NC_006570.2 1886940 1887965 R anthranilate phosphoribosyltransferase complement(1886940..1887965) Francisella tularensis subsp. tularensis SCHU S4 3190987 YP_008492920.1 CDS ST1801a NC_006570.2 1887950 1888528 R anthranilate synthase subunit II complement(1887950..1888528) Francisella tularensis subsp. tularensis SCHU S4 16744733 YP_170684.1 CDS trpE NC_006570.2 1888532 1890076 R with component II, the glutamine amidotransferase, catalyzes the formation of anthranilate from chorismate and glutamine; anthranilate synthase subunit I complement(1888532..1890076) Francisella tularensis subsp. tularensis SCHU S4 3190982 YP_170685.1 CDS trpR NC_006570.2 1890085 1890372 R Similar to TRPR_ECOLI (P03032) Trp operon repressor from E. coli (107 aa). FASTA: opt: 175 Z-score: 239.1 E(): 2e-05 Smith-Waterman score: 175; 32.584 identity in 89 aa overlap; trp operon repressor complement(1890085..1890372) Francisella tularensis subsp. tularensis SCHU S4 3190986 YP_170686.1 CDS rng NC_006570.2 1890846 1892342 R Similar to RNG_ECOLI (P25537) Ribonuclease G from E. coli (488 aa). FASTA: opt: 1439 Z-score: 1642.1 E(): 1.4e-83 Smith-Waterman score: 1439; 45.418 identity in 491 aa overlap; ribonuclease G complement(1890846..1892342) Francisella tularensis subsp. tularensis SCHU S4 3191606 FTT_t01 tRNA FTT_t01 NC_006570.2 48571 48644 D tRNA-Met 48571..48644 Francisella tularensis subsp. tularensis SCHU S4 3192264 FTT_t02 tRNA FTT_t02 NC_006570.2 84519 84601 D tRNA-Leu 84519..84601 Francisella tularensis subsp. tularensis SCHU S4 3192260 FTT_t03 tRNA FTT_t03 NC_006570.2 149738 149819 D tRNA-Tyr 149738..149819 Francisella tularensis subsp. tularensis SCHU S4 3192278 FTT_t04 tRNA FTT_t04 NC_006570.2 149824 149894 D tRNA-Gly 149824..149894 Francisella tularensis subsp. tularensis SCHU S4 3192225 FTT_t05 tRNA FTT_t05 NC_006570.2 149926 149998 D tRNA-Thr 149926..149998 Francisella tularensis subsp. tularensis SCHU S4 3192279 FTT_t06 tRNA FTT_t06 NC_006570.2 151250 151325 D tRNA-Trp 151250..151325 Francisella tularensis subsp. tularensis SCHU S4 3192285 FTT_t07 tRNA FTT_t07 NC_006570.2 287053 287127 D tRNA-Gln 287053..287127 Francisella tularensis subsp. tularensis SCHU S4 3192228 FTT_t08 tRNA FTT_t08 NC_006570.2 326622 326697 D tRNA-Glu 326622..326697 Francisella tularensis subsp. tularensis SCHU S4 3192221 FTT_t09 tRNA FTT_t09 NC_006570.2 722243 722315 D tRNA-Val 722243..722315 Francisella tularensis subsp. tularensis SCHU S4 3190978 FTT_t10 tRNA FTT_t10 NC_006570.2 774956 775040 R tRNA-Ser complement(774956..775040) Francisella tularensis subsp. tularensis SCHU S4 3192276 FTT_t11 tRNA FTT_t11 NC_006570.2 822892 822967 D tRNA-Phe 822892..822967 Francisella tularensis subsp. tularensis SCHU S4 3192266 FTT_t12 tRNA FTT_t12 NC_006570.2 822985 823061 D tRNA-Pro 822985..823061 Francisella tularensis subsp. tularensis SCHU S4 3192268 FTT_t13 tRNA FTT_t13 NC_006570.2 823070 823146 D tRNA-Arg 823070..823146 Francisella tularensis subsp. tularensis SCHU S4 3192219 FTT_t14 tRNA FTT_t14 NC_006570.2 823174 823246 D tRNA-His 823174..823246 Francisella tularensis subsp. tularensis SCHU S4 3192243 FTT_t15 tRNA FTT_t15 NC_006570.2 823256 823338 D tRNA-Leu 823256..823338 Francisella tularensis subsp. tularensis SCHU S4 3192263 FTT_t16 tRNA FTT_t16 NC_006570.2 864851 864937 D tRNA-Ser 864851..864937 Francisella tularensis subsp. tularensis SCHU S4 3192269 FTT_t17 tRNA FTT_t17 NC_006570.2 932688 932761 R tRNA-Val complement(932688..932761) Francisella tularensis subsp. tularensis SCHU S4 3190976 FTT_t18 tRNA FTT_t18 NC_006570.2 1310648 1310723 R tRNA-Ala complement(1310648..1310723) Francisella tularensis subsp. tularensis SCHU S4 8303690 FTT_t19 tRNA FTT_t19 NC_006570.2 1310741 1310814 R tRNA-Ile complement(1310741..1310814) Francisella tularensis subsp. tularensis SCHU S4 8303711 FTT_t20 tRNA FTT_t20 NC_006570.2 1313653 1313728 R tRNA-Val complement(1313653..1313728) Francisella tularensis subsp. tularensis SCHU S4 8303708 FTT_t21 tRNA FTT_t21 NC_006570.2 1313756 1313847 R tRNA-Ser complement(1313756..1313847) Francisella tularensis subsp. tularensis SCHU S4 8303718 FTT_t22 tRNA FTT_t22 NC_006570.2 1353942 1354017 R tRNA-Lys complement(1353942..1354017) Francisella tularensis subsp. tularensis SCHU S4 8303676 FTT_t23 tRNA FTT_t23 NC_006570.2 1377767 1377842 R tRNA-Ala complement(1377767..1377842) Francisella tularensis subsp. tularensis SCHU S4 8303691 FTT_t24 tRNA FTT_t24 NC_006570.2 1377860 1377933 R tRNA-Ile complement(1377860..1377933) Francisella tularensis subsp. tularensis SCHU S4 8303712 FTT_t25 tRNA FTT_t25 NC_006570.2 1451947 1452035 R tRNA-Leu complement(1451947..1452035) Francisella tularensis subsp. tularensis SCHU S4 8303700 FTT_t26 tRNA FTT_t26 NC_006570.2 1452041 1452114 R tRNA-Cys complement(1452041..1452114) Francisella tularensis subsp. tularensis SCHU S4 8303721 FTT_t27 tRNA FTT_t27 NC_006570.2 1452133 1452204 R tRNA-Gly complement(1452133..1452204) Francisella tularensis subsp. tularensis SCHU S4 8303716 FTT_t28 tRNA FTT_t28 NC_006570.2 1459457 1459529 D tRNA-Asn 1459457..1459529 Francisella tularensis subsp. tularensis SCHU S4 8303701 FTT_t29 tRNA FTT_t29 NC_006570.2 1488679 1488765 R tRNA-Leu complement(1488679..1488765) Francisella tularensis subsp. tularensis SCHU S4 8303704 FTT_t30 tRNA FTT_t30 NC_006570.2 1541116 1541192 D tRNA-Arg 1541116..1541192 Francisella tularensis subsp. tularensis SCHU S4 8303679 FTT_t31 tRNA FTT_t31 NC_006570.2 1541207 1541278 D tRNA-Gly 1541207..1541278 Francisella tularensis subsp. tularensis SCHU S4 8303714 FTT_t32 tRNA FTT_t32 NC_006570.2 1626748 1626824 D tRNA-Met 1626748..1626824 Francisella tularensis subsp. tularensis SCHU S4 8303680 FTT_t33 tRNA FTT_t33 NC_006570.2 1706190 1706263 D tRNA-Met 1706190..1706263 Francisella tularensis subsp. tularensis SCHU S4 8303710 FTT_t34 tRNA FTT_t34 NC_006570.2 1763167 1763242 R tRNA-Arg complement(1763167..1763242) Francisella tularensis subsp. tularensis SCHU S4 8303686 FTT_t35 tRNA FTT_t35 NC_006570.2 1771102 1771177 R tRNA-Ala complement(1771102..1771177) Francisella tularensis subsp. tularensis SCHU S4 8303692 FTT_t36 tRNA FTT_t36 NC_006570.2 1771195 1771268 R tRNA-Ile complement(1771195..1771268) Francisella tularensis subsp. tularensis SCHU S4 8303713 FTT_t37 tRNA FTT_t37 NC_006570.2 1890629 1890705 R tRNA-Asp complement(1890629..1890705) Francisella tularensis subsp. tularensis SCHU S4 8303719 FTT_t38 tRNA FTT_t38 NC_006570.2 1890710 1890785 R tRNA-Thr complement(1890710..1890785) Francisella tularensis subsp. tularensis SCHU S4 8303715 FTT_r01 rRNA FTT_r01 NC_006570.2 408838 408952 R 5S ribosomal RNA complement(408838..408952) Francisella tularensis subsp. tularensis SCHU S4 3190959 FTT_r02 rRNA FTT_r02 NC_006570.2 1307547 1307661 R 5S ribosomal RNA complement(1307547..1307661) Francisella tularensis subsp. tularensis SCHU S4 8303707 FTT_r03 rRNA FTT_r03 NC_006570.2 1307712 1310573 R 23S ribosomal RNA complement(1307712..1310573) Francisella tularensis subsp. tularensis SCHU S4 3190909 FTT_r04 rRNA FTT_r04 NC_006570.2 1310909 1312431 R 16S ribosomal RNA complement(1310909..1312431) Francisella tularensis subsp. tularensis SCHU S4 3192533 FTT_r05 rRNA FTT_r05 NC_006570.2 1374666 1374780 R 5S ribosomal RNA complement(1374666..1374780) Francisella tularensis subsp. tularensis SCHU S4 8303698 FTT_r06 rRNA FTT_r06 NC_006570.2 1374831 1377692 R 23S ribosomal RNA complement(1374831..1377692) Francisella tularensis subsp. tularensis SCHU S4 3190928 FTT_r07 rRNA FTT_r07 NC_006570.2 1378028 1379550 R 16S ribosomal RNA complement(1378028..1379550) Francisella tularensis subsp. tularensis SCHU S4 3192274 FTT_r08 rRNA FTT_r08 NC_006570.2 1768001 1768115 R 5S ribosomal RNA complement(1768001..1768115) Francisella tularensis subsp. tularensis SCHU S4 8303683 FTT_r09 rRNA FTT_r09 NC_006570.2 1768166 1771027 R 23S ribosomal RNA complement(1768166..1771027) Francisella tularensis subsp. tularensis SCHU S4 3192387 FTT_r10 rRNA FTT_r10 NC_006570.2 1771363 1772885 R 16S ribosomal RNA complement(1771363..1772885) Francisella tularensis subsp. tularensis SCHU S4 3192537