-- dump date 20140619_095357 -- class Genbank::CDS -- table cds_note -- id note YP_762705.1 binds to the dnaA-box as an ATP-bound complex at the origin of replication during the initiation of chromosomal replication; can also affect transcription of multiple genes including itself. YP_762706.1 DNA nucleotidyltransferase (DNA-directed) YP_762707.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs.; ISFtu1 YP_762709.1 possble periplasmic serine protease YP_762712.1 molecular chaperone that is required for the normal export of envelope proteins out of the cell cytoplasm; in Escherichia coli this proteins forms a homotetramer in the cytoplasm and delivers proteins to be exported to SecA YP_762713.1 catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs YP_762717.1 catalyzes the synthesis of acetylphosphate or propionylphosphate from acetyl-CoA or propionyl-CoA and inorganic phosphate; when using propionyl-CoA the enzyme is functioning in the anaerobic pathway catabolizing threonine to propionate YP_762718.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_762719.1 catalyzes a two-step reaction, first charging an aspartate molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; contains discriminating and non-discriminating subtypes YP_762722.1 conserved in Francisella genomes YP_762723.1 aspartate transcarbamylase YP_762724.1 four CarB-CarA dimers form the carbamoyl phosphate synthetase holoenzyme that catalyzes the production of carbamoyl phosphate; CarB is responsible for the amidotransferase activity YP_762725.1 catalyzes production of carbamoyl phosphate from bicarbonate and glutamine in pyrimidine and arginine biosynthesis pathways; forms an octamer composed of four CarAB dimers YP_762726.1 acid phosphomonoesterase YP_762727.1 catalyzes the S-adenosylmethionine-dependent transmethylation of thiopurine compounds; may be involved in selenium cycling by forming dimethylselenide and/or dimethyldiselenide YP_762728.1 Catalyzes the reversible hydrolysis of the amide bond within dihydroorotate. This metabolic intermediate is required for the biosynthesis of pyrimidine nucleotides YP_762730.1 conserved in Francisella genomes YP_762734.1 conserved in Francisella genomes YP_762738.1 OMP decarboxylase YP_762739.1 DHOdehase YP_762741.1 hydroxyphenylpyruvate synthase YP_762742.1 hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr); possible defense mechanism against a harmful effect of D-tyrosine YP_762743.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs.; ISFtu1 YP_762744.1 conserved in Francisella genomes YP_762749.1 glucose-inhibited division protein B; SAM-dependent methyltransferase; methylates the N7 position of guanosine in position 527 of 16S rRNA YP_762751.1 conserved in Francisella genomes YP_762755.1 binds directly to the 16S rRNA and is involved in post-translational inhibition of arginine and ornithine decarboxylase YP_762756.1 binds to the ribosome on the universally-conserved alpha-sarcin loop YP_762757.1 catalyzes a sulfuration reaction to synthesize 2-thiouridine at the U34 position of tRNAs YP_762758.1 conserved in Francisella genomes YP_762759.1 PDF YP_762761.1 riboflavin biosynthesis protein YP_762762.1 catalyzes the formation of riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine YP_762763.1 bifunctional riboflavin-specific deaminase and HTP reductase YP_762765.1 exports sodium by using the electrochemical proton gradient to allow protons into the cell; functions in adaptation to high salinity and alkaline pH; activity increases at higher pH; downregulated at acidic pH YP_762770.1 conserved in Francisella genomes YP_762771.1 non-folate utilizing enzyme, catalyzes the production of beta-formyl glycinamide ribonucleotide from formate, ATP, and beta-GAR and a side reaction producing acetyl phosphate and ADP from acetate and ATP; involved in de novo purine biosynthesis YP_762772.1 1,4-beta-poly-N-acetylglucosaminidase YP_762773.1 caseinolytic protease YP_762774.1 conserved in Francisella genomes YP_762775.1 catalyzes the formation of indole and glyceraldehyde 3-phosphate from indoleglycerol phosphate in tryptophan biosynthesis YP_762776.1 catalyzes the formation of L-tryptophan from L-serine and 1-(indol-3-yl)glycerol 3-phosphate YP_762778.1 Acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation YP_762779.1 conserved in Francisella genomes YP_762780.1 conserved in Francisella genomes YP_762781.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_762782.1 IglA2 YP_762783.1 IglB2 YP_762784.1 IglC2 YP_762785.1 IglD2 YP_762786.1 conserved in Francisella genomes YP_762787.1 PdpC2; conserved in Francisella genomes YP_762788.1 conserved in Francisella genomes YP_762789.1 conserved in Francisella genomes YP_762790.1 conserved in Francisella genomes YP_762793.1 conserved in Francisella genomes YP_762794.1 conserved in Francisella genomes YP_762795.1 PdpB2; conserved in Francisella genomes YP_762796.1 PdpA2; conserved in Francisella genomes YP_762798.1 branched-chain amino acid aminotransferase YP_762799.1 catalyzes the formation of phosphoenolpyruvate from pyruvate YP_762801.1 conserved in Francisella genomes YP_762802.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_762803.1 LpsA YP_762806.1 transformation of porphobilinogen to hydroxymethylbilane in porphyrin biosynthesis YP_762807.1 may be involved in chromosome condensation; overexpression in Escherichia coli protects against decondensation by camphor; overexpressing the protein results in an increase in supercoiling YP_762808.1 conserved in Francisella genomes YP_762809.1 conserved in Francisella genomes YP_762810.1 conserved in Francisella genomes YP_762815.1 carbonic anhydrase YP_762817.1 An essential enzyme in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis YP_762818.1 conserved in Francisella genomes YP_762819.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_762823.1 conserved in Francisella genomes YP_762827.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_762833.1 conserved in Francisella genomes YP_762835.1 5-dehydroshikimate reductase YP_762836.1 conserved in Francisella genomes YP_762837.1 in Escherichia coli transcription of this gene is enhanced by polyamines YP_762844.1 Involved in peptide bond synthesis; alters the affinity of the ribosome for aminoacyl-tRNA YP_762845.1 3'-5' exoribonuclease specific for small oligoribonuclotides YP_762848.1 conserved in Francisella genomes YP_762856.1 converts protoheme IX and farnesyl diphosphate to heme O YP_762859.1 pyridoxamine kinase YP_762860.1 conserved in Francisella genomes YP_762868.1 5-oxoprolyl-peptidase YP_762870.1 DNA nucleotidyltransferase (DNA-directed) YP_762871.1 valine--tRNA ligase; ValRS; converts valine ATP and tRNA(Val) to AMP PPi and valyl-tRNA(Val); class-I aminoacyl-tRNA synthetase type 1 subfamily; has a posttransfer editing process to hydrolyze mischarged Thr-tRNA(Val) which is done by the editing domain YP_762872.1 conserved in Francisella genomes YP_762873.1 conserved in Francisella genomes YP_762874.1 cytochrome b2 YP_762875.1 catalyzes the reduction of nitroaromatic compounds such as nitrofurazone, quinones and the anti-tumor agent CB1954; NAD(P)H-dependent; oxygen insensitive YP_762877.1 class II family (does not require metal); tetrameric enzyme; fumarase C; reversibly converts (S)-malate to fumarate and water; functions in the TCA cycle YP_762878.1 Charges one glutamine molecule and pairs it to its corresponding RNA trinucleotide during protein translation YP_762881.1 tetrahydrofolate dehydrogenase YP_762882.1 one of the last subunits in the assembly of the 30S subunit; absence of S2 does not inhibit assembly but results in an inactive subunit YP_762883.1 EF-Ts; functions during elongation stage of protein translation; forms a dimer; associates with EF-Tu-GDP complex and promotes exchange of GDP to GTP resulting in regeneration of the active form of EF-Tu YP_762884.1 Catalyzes the phosphorylation of UMP to UDP YP_762885.1 Rrf; Frr; ribosome-recycling factor; release factor 4; RF4; recycles ribosomes upon translation termination along with release factor RF-3 and elongation factor EF-G; A GTPase-dependent process results in release of 50S from 70S; inhibited by release factor RF-1; essential for viability; structurally similar to tRNAs YP_762886.1 catalyzes the formation of undecaprenyl pyrophosphate from isopentenyl pyrophosphate YP_762887.1 CDP-diacylglycerol synthase YP_762888.1 catalyzes the formation of dUMP from dUTP YP_762889.1 3-phosphatidyl-1'-glycerol-3??-phosphate synthase YP_762890.1 interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone; located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side; mutations in the S12 gene confer streptomycin resistance YP_762891.1 binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit YP_762892.1 EF-G; promotes GTP-dependent translocation of the ribosome during translation; many organisms have multiple copies of this gene YP_762893.1 NusE; involved in assembly of the 30S subunit; in the ribosome, this protein is involved in the binding of tRNA; in Escherichia coli this protein was also found to be involved in transcription antitermination; NusB/S10 heterodimers bind boxA sequences in the leader RNA of rrn operons which is required for antitermination; binding of NusB/S10 to boxA nucleates assembly of the antitermination complex YP_762894.1 binds directly near the 3' end of the 23S rRNA, where it nucleates assembly of the 50S subunit; essential for peptidyltransferase activity; mutations in this gene confer resistance to tiamulin YP_762895.1 L4 is important during the early stages of 50S assembly; it initially binds near the 5' end of the 23S rRNA YP_762896.1 binds third domain of 23S rRNA and protein L29; part of exit tunnel YP_762897.1 one of the primary rRNA-binding proteins; required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation YP_762898.1 protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA YP_762899.2 binds specifically to 23S rRNA during the early stages of 50S assembly; makes contact with all 6 domains of the 23S rRNA in the assembled 50S subunit and ribosome; mutations in this gene result in erythromycin resistance; located near peptidyl-transferase center YP_762900.1 forms a complex with S10 and S14; binds the lower part of the 30S subunit head and the mRNA in the complete ribosome to position it for translation YP_762901.1 located in the peptidyl transferase center and may be involved in peptidyl transferase activity; similar to archaeal L10e YP_762902.1 one of the stabilizing components for the large ribosomal subunit YP_762903.1 primary binding protein; helps mediate assembly; involved in translation fidelity YP_762904.1 binds to the 23S rRNA between the centers for peptidyl transferase and GTPase YP_762905.1 assembly initiator protein; binds to 5' end of 23S rRNA and nucleates assembly of the 50S; surrounds polypeptide exit tunnel YP_762906.1 part of 50S and 5S/L5/L18/L25 subcomplex; contacts 5S rRNA and P site tRNA; forms a bridge to the 30S subunit in the ribosome by binding to S13 YP_762907.1 located in the peptidyl transferase center and involved in assembly of 30S ribosome subunit; similar to what is observed with proteins L31 and L33, some proteins in this family contain CXXC motifs that are involved in zinc binding; if two copies are present in a genome, then the duplicated copy appears to have lost the zinc-binding motif and is instead regulated by zinc; the proteins in this group do not appear to have the zinc-binding motif YP_762908.1 binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit YP_762909.1 ribosomal protein L6 appears to have arisen as a result of an ancient gene duplication as based on structural comparison of the Bacillus stearothermophilus protein; RNA-binding appears to be in the C-terminal domain; mutations in the L6 gene confer resistance to aminoglycoside antibiotics such as gentamicin and these occur in truncations of the C-terminal domain; it has been localized to a region between the base of the L7/L12 stalk and the central protuberance YP_762910.1 binds 5S rRNA along with protein L5 and L25 YP_762911.1 located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body; contacts S4 and S8; with S4 and S12 plays a role in translational accuracy; mutations in this gene result in spectinomycin resistance YP_762912.1 L30 binds domain II of the 23S rRNA and the 5S rRNA; similar to eukaryotic protein L7 YP_762913.1 late assembly protein YP_762914.1 forms heterotrimeric complex in the membrane; in bacteria the complex consists of SecY which forms the channel pore and SecE and SecG; the SecG subunit is not essential; in bacteria translocation is driven via the SecA ATPase YP_762915.1 located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA; makes contact with the large subunit via RNA-protein interactions and via protein-protein interactions with L5; contacts P-site tRNA YP_762916.1 located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA; forms part of the Shine-Dalgarno cleft in the 70S ribosome; interacts with S7 and S18 and IF-3 YP_762917.1 primary rRNA binding protein; nucleates 30S assembly; involved in translational accuracy with proteins S5 and S12; interacts with protein S5; involved in autogeneously regulating ribosomal proteins by binding to pseudoknot structures in the polycistronic mRNA; interacts with transcription complex and functions similar to protein NusA in antitermination YP_762918.1 catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Dimerization of the alpha subunit is the first step in the sequential assembly of subunits to form the holoenzyme YP_762919.1 is a component of the macrolide binding site in the peptidyl transferase center YP_762920.1 conserved in Francisella genomes YP_762921.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_762922.1 converts 2-oxoglutarate to glutamate; in Escherichia coli this enzyme plays a role in glutamate synthesis when the cell is under energy restriction; uses NADPH; forms a homohexamer YP_762923.1 DAP decarboxylase YP_762925.1 6-O-methylguanine-DNA methyltransferase YP_762929.1 conserved in Francisella genomes YP_762933.1 conserved in Francisella genomes YP_762934.1 GTP pyrophosphokinase YP_762935.1 conserved in Francisella genomes YP_762936.1 conserved in Francisella genomes YP_762937.1 conserved in Francisella genomes YP_762943.1 molecular chaperone that is required for the normal export of envelope proteins out of the cell cytoplasm; in Escherichia coli this proteins forms a homotetramer in the cytoplasm and delivers proteins to be exported to SecA YP_762944.1 This protein performs the mismatch recognition step during the DNA repair process YP_762945.1 catalyzes the carboxylation of acetyl-CoA to malonyl-CoA; forms a tetramer composed of two alpha (AccA) and two beta (AccD) subunits; one of the two catalytic subunits that can form the acetyl CoA carboxylase enzyme together with a carrier protein YP_762946.1 conserved in Francisella genomes YP_762947.1 conserved in Francisella genomes YP_762948.1 conserved in Francisella genomes YP_762949.1 catalyzes the cleavage of the lactyl ether moiety of N-acetylmuramic acid-6-phosphate (MurNAc-6-P) to form N-acetylglucosamine-6-phosphate (GlcNAc-6-P) and lactate; involved in MurNAc dissimilation pathway YP_762950.1 conserved in Francisella genomes YP_762951.1 conserved in Francisella genomes YP_762954.1 conserved in Francisella genomes YP_762955.1 catalyzes a two-step reaction, first charging a tryptophan molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA YP_762956.1 catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A; involved in coenzyme A biosynthesis YP_762958.1 E1 component; part of pyruvate dehydrogenase; forms a complex with DlaT and LpdC YP_762959.1 acetyl-CoA:dihydrolipoamide S-acetyltransferase YP_762960.1 dehydrolipoate dehydrogenase YP_762961.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_762964.1 conserved in Francisella genomes YP_762965.1 conserved in Francisella genomes YP_762967.1 catalyzes the formation of oxalozcetate and L-glutamate from L-aspartate and 2-oxoglutarate YP_762969.1 cyclophilin YP_762970.1 catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP); functions in the nonmevalonate isoprenoid biosynthesis pathway YP_762972.1 CDP-diglycerine-serine O-phosphatidyltransferase YP_762973.1 conserved in Francisella genomes YP_762978.1 conserved in Francisella genomes YP_762980.1 catalyzes the methylthiolation of an aspartic acid residue of the S12 protein of the 30S ribosomal subunit YP_762982.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_762989.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_762991.1 UbiA prenyltransferase family catalyzes the transfer of a prenyl group to various acceptors with hydrophobic ring structures in the biosynthesis of respiratory quinones, hemes, chlorophylls, vitamin E, and shikonin YP_762992.1 chorismate lyase YP_762993.1 RNase PH; tRNA nucleotidyltransferase; forms hexamers in Bacillus subtilis; phosphoroltic 3'-5' exoribonuclease; involved in maturation of tRNA precursors and removes terminal nucleotides near CCA acceptor arms of mature tRNAs YP_762994.1 conserved in Francisella genomes YP_762995.1 conserved in Francisella genomes YP_762997.1 putative metalloprotease YP_763000.1 conserved in Francisella genomes YP_763001.1 conserved in Francisella genomes YP_763002.1 catalyzes the NAD(P)H-dependent reduction of glycerol 3-phosphate to glycerone phosphate YP_763003.1 conserved in Francisella genomes YP_763004.1 Sms; stabilizes the strand-invasion intermediate during the DNA repair; involved in recombination of donor DNA and plays an important role in DNA damage repair after exposure to mutagenic agents YP_763005.1 conserved in Francisella genomes YP_763007.1 catalyzes the formation of chorismate from 5-O-(1-carboxyvinyl)-3-phosphoshikimate in aromatic amino acid biosynthesis YP_763008.1 conserved in Francisella genomes YP_763009.1 this stereospecific enzymes reduces the R isomer of methionine sulfoxide while MsrA reduces the S form; provides protection against oxidative stress YP_763014.1 catalyzes the formation of oxalozcetate and L-glutamate from L-aspartate and 2-oxoglutarate YP_763015.1 probable cobalt (Co2+), zinc (Zn2+), cadmium (Cd2+) CDF family cation diffusion facilitator YP_763016.1 ATPase involved in DNA repair YP_763020.1 catalyzes the formation of 5,10-methenyltetrahydrofolate from 5,10-methylenetetrahydrofolate and subsequent formation of 10-formyltetrahydrofolate from 5,10-methenyltetrahydrofolate YP_763021.1 catalyzes the formation of 1-(5-phosphoribosyl)-5-aminoimidazole from 2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in purine biosynthesis YP_763023.1 2-amino-N-ribosylacetamide 5'-phosphate transformylase YP_763024.1 AIR carboxylase YP_763025.1 With PurE catalyzes the conversion of aminoimidazole ribonucleotide to carboxyaminoimidazole ribonucleotide in the de novo purine nucleotide biosynthetic pathway YP_763028.1 glucose kinase YP_763033.1 conserved in Francisella genomes YP_763034.1 glutamine phosphoribosylpyrophosphate amidotransferase YP_763036.1 conserved in Francisella genomes YP_763037.1 UDP-GlcNAc-enoylpyruvate reductase YP_763038.1 enoylpyruvate transferase YP_763039.1 EngA; essential Neisserial GTPase; synchronizes cellular events by interacting with multiple targets with tandem G-domains; overexpression in Escherichia coli suppresses rrmJ mutation; structural analysis of the Thermotoga maritima ortholog shows different nucleotide binding affinities in the two binding domains YP_763040.1 conserved in Francisella genomes YP_763045.1 conserved in Francisella genomes YP_763046.1 conserved in Francisella genomes YP_763048.1 catalyzes the ATP-dependent breakage of single-stranded DNA followed by passage and rejoining; maintains net negative superhelicity YP_763054.1 conserved in Francisella genomes YP_763055.1 lipoprotein signal peptidase; integral membrane protein that removes signal peptides from prolipoproteins during lipoprotein biosynthesis YP_763056.1 IleRS; catalyzes the formation of isoleucyl-tRNA(Ile) from isoleucine and tRNA(Ile); since isoleucine and other amino acids such as valine are similar, there are additional editing function in this enzyme; one is involved in hydrolysis of activated valine-AMP and the other is involved in deacylation of mischarged Val-tRNA(Ile); there are two active sites, one for aminoacylation and one for editing; class-I aminoacyl-tRNA synthetase family type 1 subfamily; some organisms carry two different copies of this enzyme YP_763057.1 flavokinase YP_763058.1 malic enzyme; oxaloacetate-decarboxylating; NAD-dependent; catalyzes the formation of pyruvate form malate YP_763059.1 conserved in Francisella genomes YP_763060.1 conserved in Francisella genomes YP_763063.1 methionine--tRNA ligase; MetRS; adds methionine to tRNA(Met) with cleavage of ATP to AMP and diphosphate; some MetRS enzymes form dimers depending on a C-terminal domain that is also found in other proteins such as Trbp111 in Aquifex aeolicus and the cold-shock protein CsaA from Bacillus subtilis while others do not; four subfamilies exist based on sequence motifs and zinc content YP_763071.1 conserved in Francisella genomes YP_763072.1 catalyzes the decarboxylation of phosphatidyl-L-serine to phosphatidylethanoleamine YP_763073.1 conserved in Francisella genomes YP_763074.1 NadM-Nudix subfamily; involved in creation of nicotanimide adenine dinucleotide NAD from either biosynthetic or salvage pathways YP_763075.1 N-acetylglucosamine-1-phosphate uridyltransferase YP_763076.1 Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source YP_763078.1 a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA YP_763082.1 conserved in Francisella genomes YP_763084.1 decatenates newly replicated chromosomal DNA and relaxes positive and negative DNA supercoiling YP_763085.1 enables the cleavage of the glycosidic bond in both 5'-methylthioadenosine and S-adenosylhomocysteine YP_763086.1 conserved in Francisella genomes YP_763092.1 DNA nucleotidyltransferase (DNA-directed) YP_763093.1 PDF YP_763098.1 catalyzes the transfer of a methylene carbon from the methylamine-loaded GcvH protein to tetrahydrofolate, causing the release of ammonia and the generation of reduced GcvH protein YP_763100.1 acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine; forms a heterodimer with subunit 2 to form the P protein YP_763101.1 acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine; forms a heterodimer with subunit 1 to form the P protein YP_763102.1 5-dehydroshikimate reductase YP_763103.1 alpha-dextrin endo-1,6-alpha-glucosidase YP_763104.1 catalyzes the transfer of a segment of a 1,4-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain YP_763105.1 catalyzes the interconversion of alpha-D-glucose 1-phosphate to alpha-D-glucose 6-phosphate YP_763106.1 catalyzes the formation of ADP-glucose and diphosphate from ATP and alpha-D-glucose 1-phosphate YP_763107.1 catalyzes the formation of alpha-1,4-glucan chains from ADP-glucose YP_763108.1 amylophosphorylase YP_763109.1 amylomaltase; acts to release glucose from maltodextrins YP_763110.1 glycine--tRNA ligase alpha chain; GlyRS; class II aminoacyl tRNA synthetase; tetramer of alpha(2)beta(2); catalyzes a two-step reaction; first charging a glycine molecule by linking its carboxyl group to the alpha-phosphate of ATP; second by transfer of the aminoacyl-adenylate to its tRNA YP_763111.1 murE synthetase YP_763114.1 conserved in Francisella genomes YP_763115.1 catalyzes the formation of L-threonine from O-phospho-L-homoserine YP_763116.1 S-adenosyl-L-methionine decarboxylase YP_763117.1 catalyzes the formation of spermidine from putrescine and S-adenosylmethioninamine YP_763118.1 catalyzes the formation of 2,3=diacylglucosamine 1-phosphate from UDP-2,3=diacylglucosamine YP_763119.1 involved in fifth step of pyrimidine biosynthesis; converts orotidine 5'-phosphate and diphosphate to orotate and 5-phospho-alpha-D-ribose 1-diphosphate YP_763122.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763123.1 conserved in Francisella genomes YP_763125.1 conserved in Francisella genomes YP_763130.1 works in conjunction with MinC and MinD to enable cell division at the midpoint of the long axis of the cell YP_763133.1 in Escherichia coli BM108, a mutation that results in lack of L33 synthesis had no effect on ribosome synthesis or function; there are paralogous genes in several bacterial genomes, and a CXXC motif for zinc binding and an upstream regulation region of the paralog lacking this motif that are regulated by zinc similar to other ribosomal proteins like L31; the proteins in this group lack the CXXC motif YP_763134.1 required for 70S ribosome assembly YP_763135.1 conserved in Francisella genomes YP_763139.1 fumarase YP_763142.1 type III restriction enzyme YP_763143.1 Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine YP_763144.1 conserved in Francisella genomes YP_763145.1 DNA gyrase YP_763146.1 catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate YP_763149.1 adds the O-linked and N-linked 3(R)-hydroxy fatty acids to the glucosamine disaccharide during lipid A biosynthesis YP_763150.1 in Pseudomonas aeruginosa this enzyme is a trimer of dimers; essential for membrane formation; performs third step of type II fatty acid biosynthesis; catalyzes dehydration of (3R)-hydroxyacyl-ACP to trans-2-acyl-ACP YP_763151.1 catalyzes the addition of (R)-3-hydroxytetradecanoyl to the glucosamine disaccharide in lipid A biosynthesis YP_763152.1 catalyzes the formation of lipid A disaccharide from UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate, lipid A disaccharide is a precursor of lipid A that anchors LPS to the OM YP_763153.1 conserved in Francisella genomes YP_763157.1 CDP-diglyceride-choline O-phosphatidyltransferase YP_763158.1 farnesyl diphosphate synthetase YP_763159.1 3-deoxy-D-manno-octulosonic-acid transferase KdtA YP_763160.1 HAM1-like protein; Rec-dependent growth; RgdB; yggV; it is suspected that this protein functions to remove misincorporated bases such as xanthine or hypoxanthine YP_763161.1 P5CR YP_763163.1 bacterial leader peptidase I YP_763164.1 cytoplasmic enzyme involved in processing rRNA and some mRNAs; substrates typically have dsRNA regions; forms a homodimer; have N-terminal nuclease and C-terminal RNA-binding domains; requires magnesium as preferred ion for activity YP_763165.1 catalyzes isomerization of specific uridines in RNA to pseudouridine; responsible for residues in T loops of many tRNAs YP_763166.1 VacB YP_763170.1 CFA synthase YP_763175.1 outer membrane protein 26; conserved in Francisella genomes YP_763176.1 conserved in Francisella genomes YP_763181.1 niacin ribonucleotidase YP_763185.1 Catalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation YP_763186.1 beta-hydroxyacyl dehydrogenase YP_763187.2 functions in fatty acid oxidation; converts acyl-CoA and FAD to FADH2 and delta2-enoyl-CoA YP_763188.1 acyl-activating enzyme YP_763190.1 dual-cofactor-specific isocitrate dehydrogenase YP_763191.1 conserved in Francisella genomes YP_763195.1 galactowaldenase YP_763197.1 UDP-N-acetyl-D-mannosaminuronate dehydrogenase YP_763198.1 galactowaldenase YP_763201.1 asparagine synthetase (glutamine-hydrolyzing) YP_763202.1 pyridoxal phosphate-dependent sugar transaminase YP_763204.1 O-antigen transporter YP_763206.1 dTDP-glucose diphosphorylase YP_763207.1 GDP-mannose phosphorylase YP_763208.1 phosphomannose mutase YP_763209.1 An RNA-DNA helicase that actively releases nascent mRNAs from paused transcription complexes YP_763211.1 exopolypase YP_763215.1 catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Dimerization of the alpha subunit is the first step in the sequential assembly of subunits to form the holoenzyme YP_763224.1 DPN kinase YP_763226.1 tRNA (guanine-N(7)-)-methyltransferase; catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA by transferring the methyl residue from S-adenosyl-L-methionine YP_763234.1 conserved in Francisella genomes YP_763237.1 conserved in Francisella genomes YP_763238.1 conserved in Francisella genomes YP_763239.1 conserved in Francisella genomes YP_763240.1 catalyzes the formation of prolyl-tRNA(Pro) from proline and tRNA(Pro) YP_763246.1 hydroxymethylbilane hydrolyase (cyclizing) YP_763247.1 conserved in Francisella genomes YP_763248.1 conserved in Francisella genomes YP_763249.1 glyoxalase II YP_763250.1 conserved in Francisella genomes YP_763252.1 conserved in Francisella genomes YP_763254.1 conserved in Francisella genomes YP_763256.1 glutamate (reduced nicotinamide adenine dinucleotide phosphate) synthase YP_763257.1 similar to Escherichia coli exonuclease V YP_763261.1 similar to Escherichia coli exonuclease V YP_763262.1 similar to Escherichia coli exonuclease V YP_763263.1 type III; catalyzes the formation of (R)-4'-phosphopantothenate from (R)-pantothenate in coenzyme A biosynthesis; type III pantothenate kinases are not subject to feedback inhibition from coenzyme A and have a high Km for ATP YP_763264.1 Converts L-aspartate to beta-alanine and provides the major route of beta-alanine production in bacteria. Beta-alanine is essential for the biosynthesis of pantothenate (vitamin B5) YP_763265.1 pantoate activating enzyme YP_763266.1 catalyzes the formation of tetrahydrofolate and 2-dehydropantoate from 5,10-methylenetetrahydrofolate and 3-methyl-2-oxobutanoate YP_763268.1 DNA joinase YP_763272.1 conserved in Francisella genomes YP_763273.1 NAD(+) synthetase (glutamine-hydrolyzing) YP_763276.1 possible multidrug efflux transporter YP_763278.1 acyl-activating enzyme YP_763280.1 in Escherichia coli RsmE methylates the N3 position of the U1498 base in 16S rRNA; cells lacking this function can grow, but are outcompeted by wild-type; SAM-dependent m(3)U1498 methyltransferase YP_763282.1 conserved in Francisella genomes YP_763283.1 mycobacterial cell entry protein (mce) YP_763287.1 pseudouridine synthase YP_763290.1 catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate YP_763292.1 conserved in Francisella genomes YP_763294.1 conserved in Francisella genomes YP_763296.1 conserved in Francisella genomes YP_763300.1 catalyzes the formation of 3-phosphonooxypyruvate from 3-phospho-D-glycerate in serine biosynthesis; can also reduce alpha ketoglutarate to form 2-hydroxyglutarate YP_763301.1 ThyA; catalyzes formation of dTMP and 7,8-dihydrofolate from 5,10-methylenetetrahydrofolate and dUMP; involved in deoxyribonucleotide biosynthesis; there are 2 copies in some Bacilli, one of which appears to be phage-derived YP_763302.1 transfers the N-acyl diglyceride moiety to the prospective N-terminal cysteine in prolipoprotein YP_763305.1 conserved in Francisella genomes YP_763310.1 5,10-methenyltetrahydrofolate synthetase YP_763314.1 conserved in Francisella genomes YP_763315.1 Synthesizes oQ from preQ1 in a single S-adenosylmethionine-requiring step YP_763318.1 aldoketomutase YP_763319.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763320.1 Catalyzes D-ribose 5-phosphate --> D-ribulose 5-phosphate in the nonoxidative branch of the pentose phosphate pathway YP_763321.1 conserved in Francisella genomes YP_763322.1 conserved in Francisella genomes YP_763323.1 GidA; glucose-inhibited cell division protein A; involved in the 5-carboxymethylaminomethyl modification (mnm(5)s(2)U) of the wobble uridine base in some tRNAs YP_763324.1 conserved in Francisella genomes YP_763325.1 conserved in Francisella genomes YP_763327.1 similar to Escherichia coli exonuclease I YP_763328.1 The UvrABC repair system catalyzes the recognition and processing of DNA lesions. The beta-hairpin of the Uvr-B subunit is inserted between the strands, where it probes for the presence of a lesion YP_763335.1 bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides YP_763337.1 conserved in Francisella genomes YP_763338.1 TtcA; YdaO; catalyzes the thiolation of cytosine 32 in specific tRNAs YP_763339.1 binds to ssrA RNA (tmRNA) and is required for its successful binding to ribosomes; also appears to function in the trans-translation step by promoting accommodation of tmRNA into the ribosomal A site; SmpB protects the tmRNA from RNase R degradation in Caulobacter crescentus; both the tmRNA and SmpB are regulated in cell cycle-dependent manner; functions in release of stalled ribosomes from damaged mRNAs and targeting proteins for degradation YP_763344.1 gamma-glutamyltranspeptidase YP_763347.1 conserved in Francisella genomes YP_763348.1 conserved in Francisella genomes YP_763349.1 conserved in Francisella genomes YP_763350.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763353.1 L-glutamine amidohydrolase YP_763355.1 glutamine amidotransferase YP_763356.1 aspartate aminotransferase YP_763357.1 Era; Escherichia coli Ras-like protein; Bex; Bacillus Era-complementing segment; essential protein in Escherichia coli that is involved in many cellular processes; GTPase; binds the cell membrane through apparent C-terminal domain; mutants are arrested during the cell cycle; Streptococcus pneumoniae Era binds to RNA and Escherichia coli Era binds 16S rRNA and 30S ribosome YP_763358.1 conserved in Francisella genomes YP_763360.1 essential enzyme that recycles AMP in active cells; converts ATP and AMP to two molecules of ADP YP_763361.1 conserved in Francisella genomes YP_763366.1 type I enzyme similar to type II but differentially regulated; major shikimate kinase in fully repressed cells; catalyzes the formation of shikimate 3-phosphate from shikimate in aromatic amino acid biosynthesis YP_763367.1 catalyzes the formation of 3-dehydroquinate from 3-deoxy-arabino-heptulonate 7-phosphate; functions in aromatic amino acid biosynthesis YP_763368.1 conserved in Francisella genomes YP_763369.1 conserved in Francisella genomes YP_763371.1 proline utilization protein A; multifunctional protein that functions in proline catabolism in the first two enzymatic steps resulting in the conversion of proline to glutamate; in Escherichia coli this protein self regulates transcription via a DNA-binding domain at the N-terminus but the protein from Pseudomonas does not have this domain YP_763376.1 conserved in Francisella genomes YP_763380.1 protoheme ferro-lyase; catalyzes the insertion of a ferrous ion into protoporphyrin IX to form protoheme; involved in protoheme biosynthesis; in some organisms this protein is membrane-associated while in others it is cytosolic YP_763381.1 conserved in Francisella genomes YP_763382.1 conserved in Francisella genomes YP_763387.1 catalyze the formation of cyanophycin which may act to store excess nitrogen YP_763389.1 catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate YP_763394.1 Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr) YP_763398.1 part of the preprotein secretory system; when complexed with proteins SecF and YajC, SecDFyajC stimulates the proton motive force-driven protein translocation, and appears to be required for the release of mature proteins from the extracytoplasmic side of the membrane YP_763399.1 forms a complex with SecD and YajC; SecDFyajC stimulates the proton motive force-driven protein translocation; seems to modulate the cycling of SecA by stabilizing its membrane-inserted state and appears to be required for the release of mature proteins from the extracytoplasmic side of the membrane; in some organisms, such as Bacillus subtilis, SecD is fused to SecF YP_763400.1 conserved in Francisella genomes YP_763401.1 binds with the catalytic core of RNA polymerase to produce the holoenzyme; this sigma factor is responsible for the expression of heat shock promoters YP_763402.1 3-enol-pyruvoylshikimate-5-phosphate synthase YP_763403.1 conserved in Francisella genomes YP_763404.1 endoribonuclease H YP_763407.1 conserved in Francisella genomes YP_763408.1 1-aminocyclopropane carboxylic acid deaminase YP_763412.1 possible sugar transporter YP_763413.1 conserved in Francisella genomes YP_763414.1 conserved in Francisella genomes YP_763415.1 catalyzes the conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate into 4-hydroxy-3-methyl-2-en-1-yl diphosphate; involved in isoprenoid synthesis YP_763419.1 cephalosporinase YP_763425.1 catalyzes the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine from N6-(dimethylallyl)adenosine (i(6)A) YP_763427.1 catalyzes the formation of thymidine 5'-phosphate from thymidine YP_763428.1 Tig; RopA; peptidyl-prolyl cis/trans isomerase; promotes folding of newly synthesized proteins; binds ribosomal 50S subunit; forms a homodimer YP_763429.1 hydrolyzes proteins to small peptides; with the ATPase subunits ClpA or ClpX, ClpP degrades specific substrates YP_763430.1 binds and unfolds substrates as part of the ClpXP protease YP_763431.1 ATP-dependent protease La YP_763432.1 histone-like protein HU form B YP_763434.1 IPP transferase; isopentenyltransferase; involved in tRNA modification; in Escherichia coli this enzyme catalyzes the addition of a delta2-isopentenyl group from dimethylallyl diphosphate to the N6-nitrogen of adenosine adjacent to the anticodon of tRNA species that read codons starting with uracil; further tRNA modifications may occur; mutations in miaA result in defects in translation efficiency and fidelity YP_763439.1 phosphotyrosine phosphatase YP_763440.1 binds guanine nucleotides; in Escherichia coli depletion results in defective cell division and filamentation; in Bacillus subtilis this gene is essential YP_763441.1 conserved in Francisella genomes YP_763442.1 conserved in Francisella genomes YP_763443.1 acetohydroxy acid synthetase YP_763444.1 acetohydroxy acid isomeroreductase YP_763445.1 superfamily II helicase YP_763447.1 conserved in Francisella genomes YP_763449.1 AP endonuclease class I YP_763451.1 cofactor involved in the reduction of disulfides YP_763453.1 catalyzes the radical-mediated insertion of two sulfur atoms into an acyl carrier protein (ACP) bound to an octanoyl group to produce a lipoyl group YP_763456.1 endonuclease; resolves Holliday structures; forms a complex of RuvABC; the junction binding protein RuvA forms a hexameric ring along with the RuvB helicase and catalyzes branch migration; RuvC then interacts with RuvAB to resolve the Holliday junction by nicking DNA strands of like polarity YP_763458.1 plays an essential role in ATP-dependent branch migration of the Holliday junction YP_763460.1 conserved in Francisella genomes YP_763463.1 catalyzes the formation of histamine from L-histidine YP_763470.1 phosphoribosyl diphosphate synthetase YP_763471.1 the Ctc family of proteins consists of two types, one that contains the N-terminal ribosomal protein L25 domain only which in Escherichia coli binds the 5S rRNA while a subset of proteins contain a C-terminal extension that is involved in the stress response YP_763473.1 conserved in Francisella genomes YP_763474.1 translation-associated GTPase; the crystal structure of the Haemophilus influenzae YchF protein showed similarity to the yeast structure (PDB: 1NI3); fluorescence spectroscopy revealed nucleic acid binding; the yeast protein YBR025c interacts with the translation elongation factor eEF1 YP_763475.1 Enables the recycling of peptidyl-tRNAs produced at termination of translation YP_763476.1 cephalosporinase YP_763479.1 catalyzes the conversion of NADPH to NADH YP_763481.1 heat shock protein involved in degradation of misfolded proteins YP_763482.1 heat shock protein involved in degradation of misfolded proteins YP_763483.1 conserved in Francisella genomes YP_763485.1 catalyzes the formation of tyrosyl-tRNA(Tyr) from tyrosine and tRNA(Tyr) YP_763487.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763488.1 cytochrome b2 YP_763494.1 type I restriction enzyme YP_763497.1 conserved in Francisella genomes YP_763498.1 involved in translesion DNA polymerization with beta clamp of polymerase III; belongs to Y family of polymerases; does not contain proofreading function YP_763501.1 ribonucleotide reductase YP_763503.1 Catalyzes the rate-limiting step in dNTP synthesis YP_763504.1 Catalyzes the reversible oxidation of malate to oxaloacetate YP_763505.1 conserved in Francisella genomes YP_763508.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763513.1 conserved in Francisella genomes YP_763514.1 conserved in Francisella genomes YP_763515.1 DNA nucleotidyltransferase (DNA-directed) YP_763524.1 CMP kinase YP_763525.1 catalyzes the formation of 3-phosphonooxypyruvate and glutamate from O-phospho-L-serine and 2-oxoglutarate; required both in major phosphorylated pathway of serine biosynthesis and in the biosynthesis of pyridoxine YP_763527.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763529.1 catalyzes the conversion of the propionic acid groups of rings I and III to vinyl groups during heme synthesis YP_763531.1 binds cooperatively with S18 to the S15-16S complex, allowing platform assembly to continue with S11 and S21 YP_763532.1 binds as a heterodimer with protein S6 to the central domain of the 16S rRNA; helps stabilize the platform of the 30S subunit YP_763533.1 in Escherichia coli this protein is wrapped around the base of the L1 stalk YP_763537.1 pseudouridine synthase YP_763538.1 conserved in Francisella genomes YP_763539.1 acetyl-CoA:dihydrolipoamide S-acetyltransferase YP_763542.1 conserved in Francisella genomes YP_763543.1 conserved in Francisella genomes YP_763544.1 conserved in Francisella genomes YP_763545.1 conserved in Francisella genomes YP_763546.1 conserved in Francisella genomes YP_763547.1 conserved in Francisella genomes YP_763549.1 cyclophilin YP_763550.1 TtcA; YdaO; catalyzes the thiolation of cytosine 32 in specific tRNAs YP_763553.1 D-alanyl-D-alanine carboxypeptidase YP_763554.1 a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA YP_763558.1 beta-NADH dehydrogenase dinucleotide YP_763560.1 lipoyl/octanoyltransferase; catalyzes the transfer of the lipoyl/octanoyl moiety of lipoyl/octanoyl-ACP onto lipoate-dependent enzymes like pyruvate dehydrogenase and the glycine cleavage system H protein YP_763562.1 D-alanyl-D-alanine carboxypeptidase YP_763563.1 diphosphate phosphohydrolase YP_763565.1 conserved in Francisella genomes YP_763566.1 conserved in Francisella genomes YP_763570.1 mediates pseudouridylation (positions 38, 39, 40) at the tRNA anticodon region which contributes to the structural stability YP_763571.1 contains glutamine-hydrolyzing domain and glutamine amidotransferase; GMP-binding domain; functions to produce GMP from XMP in the IMP pathway YP_763572.1 catalyzes the formation of 1-deoxy-D-xylulose 5-phosphate from pyruvate and D-glyceraldehyde 3-phosphate YP_763577.1 promotes strand exchange during homologous recombination; RuvAB complex promotes branch migration; RuvABC complex scans the DNA during branch migration and resolves Holliday junctions at consensus sequences; forms hexameric rings around opposite DNA arms; requires ATP for branch migration and orientation of RuvAB complex determines direction of migration YP_763580.1 conserved in Francisella genomes YP_763586.1 this fusion consists of methionine sulfoxide B reductase at the N-terminus and A at the C-terminus; A and B are stereospecific enzymes that recognize the damaged produces of oxidative stress, S and R epimers of methionine sulfoxide, respectively; a fusion protein of these enzymes with thioredoxin provides protection against oxidative stress in Neisseria gonorrhoeae YP_763589.1 conserved in Francisella genomes YP_763591.1 conserved in Francisella genomes YP_763592.1 Catalyzes a two-step reaction, first charging an alanyl molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA YP_763594.1 cytosol aminopeptidase YP_763595.1 dihydroxyacetone transferase YP_763602.1 Required for efficient pilin antigenic variation YP_763603.1 conserved in Francisella genomes YP_763612.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763615.1 inositol 1-phosphatase YP_763619.1 beta-ketoacyl-ACP synthase YP_763620.1 carries the fatty acid chain in fatty acid biosynthesis YP_763623.1 beta-ketoacyl-ACP synthase YP_763624.1 involved in acylation of glycerol-3-phosphate to form 1-acyl-glycerol-3 phosphate for use in phospholipid biosynthesis; functions with PlsY YP_763625.1 some L32 proteins have zinc finger motifs consisting of CXXC while others do not YP_763627.1 catalyzes the formation of ribose 5-phosphate and xylulose 5-phosphate from sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate; can transfer ketol groups between several groups; in Escherichia coli there are two tkt genes, tktA expressed during exponential growth and the tktB during stationary phase YP_763628.1 GAPDH YP_763629.1 Converts 3-phospho-D-glycerate to 3-phospho-D-glyceroyl phosphate during the glycolysis pathway YP_763630.1 phosphoenol transphosphorylase YP_763631.1 class II aldolase; catalyzes the reversible aldol condensation of dihydroxyacetonephosphate and glyceraldehyde 3-phosphate in the Calvin cycle, glycolysis and gluconeogenesis YP_763632.1 IglA1 YP_763633.1 IglB1 YP_763634.1 IglC1 YP_763635.1 IglD1 YP_763636.1 conserved in Francisella genomes YP_763637.1 PdpC1; conserved in Francisella genomes YP_763638.1 conserved in Francisella genomes YP_763639.1 conserved in Francisella genomes YP_763640.1 conserved in Francisella genomes YP_763641.1 conserved in Francisella genomes YP_763643.1 conserved in Francisella genomes YP_763644.1 conserved in Francisella genomes YP_763645.1 conserved in Francisella genomes YP_763646.1 PdpB1; conserved in Francisella genomes YP_763647.1 PdpA1; conserved in Francisella genomes YP_763650.1 beta-thionase YP_763651.1 conserved in Francisella genomes YP_763652.1 in Escherichia coli this protein is involved in the biosynthesis of the hypermodified nucleoside 5-methylaminomethyl-2-thiouridine, which is found in the wobble position of some tRNAs and affects ribosomal frameshifting; shows potassium-dependent dimerization and GTP hydrolysis; also involved in regulation of glutamate-dependent acid resistance and activation of gadE YP_763653.1 conserved in Francisella genomes YP_763658.1 RNH2; RNase HII; binds manganese; endonuclease which specifically degrades the RNA of RNA-DNA hybrids YP_763660.1 stringent starvation protein subunit B YP_763661.1 stringent starvation protein subunit A YP_763662.2 forms a direct contact with the tRNA during translation YP_763663.1 in Escherichia coli this protein is one of the earliest assembly proteins in the large subunit YP_763666.1 with DnaK and DnaJ acts in response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins; may act as a thermosensor YP_763667.1 heat shock protein 70; assists in folding of nascent polypeptide chains; refolding of misfolded proteins; utilizes ATPase activity to help fold; co-chaperones are DnaJ and GrpE; multiple copies in some bacteria YP_763668.1 chaperone Hsp40; co-chaperone with DnaK; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion YP_763670.1 conserved in Francisella genomes YP_763671.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763673.1 catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a heterotetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 1 subfamily YP_763674.1 catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a tetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 2 subfamily YP_763678.1 conserved in Francisella genomes YP_763682.1 Catalyzes the formation of dUTP from dCTP in thymidylate biosynthesis YP_763683.1 conserved in Francisella genomes YP_763684.1 leucine--tRNA ligase; LeuRS; class-I aminoacyl-tRNA synthetase; charges leucine by linking carboxyl group to alpha-phosphate of ATP and then transfers aminoacyl-adenylate to its tRNA; due to the large number of codons that tRNA(Leu) recognizes, the leucyl-tRNA synthetase does not recognize the anticodon loop of the tRNA, but instead recognition is dependent on a conserved discriminator base A37 and a long arm; an editing domain hydrolyzes misformed products; in Methanothermobacter thermautotrophicus this enzyme associates with prolyl-tRNA synthetase YP_763687.1 conserved in Francisella genomes YP_763688.1 conserved in Francisella genomes YP_763690.1 crossover junction endoribonuclease YP_763692.1 conserved in Francisella genomes YP_763696.1 conserved in Francisella genomes YP_763703.1 with SufCD activates cysteine desulfurase SufS YP_763705.1 involved in potassium uptake; found to be peripherally associated with the inner membrane in Escherichia coli; contains an NAD-binding domain YP_763709.1 stimulates the activities of the other two initiation factors, IF-2 and IF-3 YP_763711.1 conserved in Francisella genomes YP_763713.1 catalyzes the formation of 3-deoxy-D-arabino-hept-2-ulosonate 7 phosphate from phosphoenolpyruvate and D-erythrose 4-phosphate, phenylalanine sensitive YP_763714.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763716.1 similar to Escherichia coli exonuclease III YP_763719.1 conserved in Francisella genomes YP_763720.1 catalyzes the reduction of 2 glutathione to glutathione disulfide; maintains high levels of reduced glutathione in the cytosol; involved in redox regulation and oxidative defense YP_763724.1 involved in the first step of tetrahydrofolate biosynthesis; catalyzes the formation of formate and 2-amino-4-hydroxy-6-(erythro-1,2, 3-trihydroxypropyl)dihydropteridine triphosphate from GTP and water; forms a homopolymer YP_763729.1 anthranilate synthase component I YP_763730.1 acyl-activating enzyme YP_763732.1 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase YP_763735.1 7,8-diamino-pelargonic acid aminotransferase YP_763736.1 biotin synthetase YP_763737.1 7-KAP synthetase YP_763739.1 DTB synthetase YP_763740.1 acetyl-CoA carboxylase biotin holoenzyme synthetase YP_763742.1 converts acetoacetate to acetone and carbon dioxide YP_763743.1 beta-hexosaminidase YP_763744.1 Converts (S)-4-amino-5-oxopentanoate to 5-aminolevulinate during the porphyrin biosynthesis pathway YP_763745.1 catalyzes the second step in the glutathione biosynthesis pathway, where it synthesizes ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione YP_763746.1 formylmethionyl-transfer ribonucleic synthetase YP_763747.1 conserved in Francisella genomes YP_763748.1 catalyzes the N2-methyl guanosine modification of the G2445 residue of 23S rRNA YP_763752.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763753.1 conserved in Francisella genomes YP_763754.1 short-chain dehydrogenase/reductase YP_763755.1 conserved in Francisella genomes YP_763756.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763757.1 conserved in Francisella genomes YP_763759.1 phenol hydroxylase YP_763760.2 RpmE; there appears to be two types of ribosomal proteins L31 in bacterial genomes; some contain a CxxC motif while others do not; Bacillus subtilis has both types; the proteins in this cluster have the CXXC motif; RpmE is found in exponentially growing Bacilli while YtiA was found after exponential growth; expression of ytiA is controlled by a zinc-specific transcriptional repressor; RpmE contains one zinc ion and a CxxC motif is responsible for this binding; forms an RNP particle along with proteins L5, L18, and L25 and 5S rRNA; found crosslinked to L2 and L25 and EF-G; may be near the peptidyltransferase site of the 50S ribosome YP_763761.1 gamma-glutamyl-L-cysteine synthetase YP_763763.1 conserved in Francisella genomes YP_763765.1 7,8-dihydrofolate synthetase YP_763767.1 catalyzes the formation of nucleoside triphosphate from ATP and nucleoside diphosphate YP_763768.1 CTP synthase; cytidine triphosphate synthetase; catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; in Escherichia coli this enzyme forms a homotetramer YP_763769.1 conserved in Francisella genomes YP_763770.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763771.1 conserved in Francisella genomes YP_763776.1 Catalyzes the conversion of ATP and pantetheine 4'-phosphate to diphosphate and 3'-dephospho-coA YP_763779.1 selenocysteine beta-lyase YP_763780.1 L-hydroxyaminoacid dehydratase YP_763785.1 conserved in Francisella genomes YP_763787.1 glycyl translase YP_763790.1 conserved in Francisella genomes YP_763792.1 glucose-1-phosphate uridylyltransferase YP_763794.1 conserved in Francisella genomes YP_763795.1 a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA YP_763796.1 conserved in Francisella genomes YP_763804.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763807.1 ribosome-associated heat shock protein Hsp15 YP_763809.1 conserved in Francisella genomes YP_763812.1 conserved in Francisella genomes YP_763813.1 conserved in Francisella genomes YP_763815.1 nicotinate-nucleotide pyrophosphorylase (carboxylating) YP_763816.1 3 different subfamilies; catalyzes the formation of quinolinate from iminoaspartate and dihydroxyacetone phosphate YP_763817.1 Essential for recycling GMP and indirectly, cGMP YP_763819.1 cyclophilin YP_763820.1 catalyzes the interconversion of UDP-galactose and galactose-1-P with UDP-galactose and glucose-1-P YP_763823.1 CMP-2-keto-3-deoxyoctulosonic acid synthetase; catalyzes the formation of CMP-3-deoxy-D-manno-octulosonate from CTP and 3-deoxy-D-manno-octulosonate which is incorporated into LPS YP_763825.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763827.1 binds directly to 23S ribosomal RNA prior to in vitro assembly of the 50S ribosomal subunit YP_763829.1 elongation factor (EF) YP_763830.1 catalyzes a two-step reaction, first charging a threonine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; catalyzes the formation of threonyl-tRNA(Thr) from threonine and tRNA(Thr) YP_763831.1 conserved in Francisella genomes YP_763832.1 conserved in Francisella genomes YP_763834.1 UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase; involved in cell wall formation; inner membrane-associated; last step of peptidoglycan synthesis YP_763836.1 involved in a recombinational process of DNA repair, independent of the recBC complex YP_763837.1 (ppGpp)ase YP_763840.1 probable poly-gamma-glutamate synthesis protein YP_763842.1 L-asparaginase YP_763843.1 possible cyanophycinase YP_763849.1 conserved in Francisella genomes YP_763854.1 galactowaldenase YP_763856.1 D-ribulose phosphate-3-epimerase YP_763857.1 arabinose phosphate isomerase YP_763859.1 conserved in Francisella genomes YP_763860.1 enoyl-ACP reductase YP_763862.1 conserved in Francisella genomes YP_763863.1 conserved in Francisella genomes YP_763864.1 The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision YP_763865.1 RNase D YP_763868.1 involved in the peptidyltransferase reaction during translation YP_763870.1 functions in protein export; can interact with acidic membrane phospholipids and the SecYEG protein complex; binds to preproteins; binds to ATP and undergoes a conformational change to promote membrane insertion of SecA/bound preprotein; ATP hydrolysis appears to drive release of the preprotein from SecA and deinsertion of SecA from the membrane; additional proteins SecD/F/YajC aid SecA recycling; exists in an equilibrium between monomers and dimers; may possibly form higher order oligomers; proteins in this cluster correspond SecA1; SecA2 is not essential and seems to play a role in secretion of a subset of proteins YP_763872.1 conserved in Francisella genomes YP_763873.1 inosine phosphorylase YP_763874.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763877.1 necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites; arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus YP_763879.1 functions in sugar metabolism in glycolysis and the Embden-Meyerhof pathways (EMP) and in gluconeogenesis; catalyzes reversible isomerization of glucose-6-phosphate to fructose-6-phosphate; member of PGI family YP_763880.1 thiamine kinase YP_763881.1 IMP oxidoreductase YP_763890.1 pyrimidine phosphorylase YP_763891.1 cytidine aminohydrolase YP_763893.1 catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate YP_763894.1 catalyzes a two-step reaction, first charging a serine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA YP_763895.1 D-fructokinase YP_763897.1 conserved in Francisella genomes YP_763898.1 conserved in Francisella genomes YP_763899.1 in Escherichia coli the CydCD ABC transporter exports cysteine and glutathione into the periplasm in order to maintain redox balance; important for cytochrome bd and c YP_763900.1 in Escherichia coli the CydCD ABC transporter exports cysteine and glutathione into the periplasm in order to maintain redox balance; important for cytochrome bd and c YP_763902.1 translation initiation inhibitor YP_763904.1 proline/betaine transporter YP_763905.1 deoxy-GTPase YP_763907.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763908.1 possible D-Ala-D-Ala carboxypeptidase C YP_763910.1 glycerophosphoryl diester phosphodiesterase YP_763912.1 conserved in Francisella genomes YP_763913.1 Catalyzes the formation of uracil and 5-phospho-alpha-D-ribosy 1-diphosphate from UMP and diphosphate YP_763915.1 catalyzes the formation of 2-amino-3-oxobutanoate from acetyl-CoA and glycine YP_763916.1 converts threonine and NAD to 1,2-amino-3-oxobutanoate and NADH; functions in threonine catabolism YP_763918.1 4-diphosphocytidyl-2-C-methyl-D-erythritol synthase YP_763920.1 enolase; catalyzes the formation of phosphoenolpyruvate from 2-phospho-D-glycerate in glycolysis YP_763924.1 in Escherichia coli this enzyme catalyzes the SAM-dependent methylation of U1939 in the 23S ribomal RNA; binds an iron-sulfur cluster [4Fe-4S] YP_763925.1 conserved in Francisella genomes YP_763926.1 DNA-dependent RNA polymerase YP_763927.1 functions in pyrimidine salvage; pyrimidine ribonucleoside kinase; phosphorylates nucleosides or dinucleosides to make UMP or CMP using ATP or GTP as the donor YP_763928.1 catalyzes the formation of 2-dehydro-3-deoxy-D-octonate 8-phosphate from phosphoenolpyruvate and D-arabinose 5-phosphate in LPS biosynthesis YP_763929.1 essential respiratory protein A; may be involved in the transfer of iron-sulfur clusters; essential for growth using oxygen or alternate electron acceptors YP_763930.1 polynucleotide phosphorylase YP_763931.1 primary rRNA binding protein; helps nucleate assembly of 30S; binds directly to the 16S rRNA and an intersubunit bridge to the 23S rRNA; autoregulates translation through interactions with the mRNA leader sequence YP_763932.1 penicillin binding protein (3 or 1B) YP_763933.1 conserved in Francisella genomes YP_763936.1 Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases YP_763937.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763938.2 with PdxST is involved in the biosynthesis of pyridoxal 5'-phosphate; PdxT catalyzes the hydrolysis of glutamine to glutamate and ammonia; PdxS utilizes the ammonia to synthesize pyridoxal 5'-phosphate YP_763939.1 with PdxT forms pyridoxal 5'-phosphate from glutamine, either ribose 5-phosphate or ribulose 5-phosphate, and either glyceraldehyde 3-phosphate or dihydroxyacetone phosphate YP_763940.1 DNA gyrase YP_763944.1 conserved in Francisella genomes YP_763945.1 conserved in Francisella genomes YP_763946.1 conserved in Francisella genomes YP_763947.1 catalyzes the interconversion of succinyl-CoA and succinate YP_763948.1 succinate thiokinase YP_763949.1 conserved in Francisella genomes YP_763952.1 alanine:alanine ligase (ADP-forming) YP_763953.1 L-asparaginase YP_763955.1 arabinose permease YP_763958.1 NADP--thioredoxin reductase YP_763960.1 conserved in Francisella genomes YP_763962.1 conserved in Francisella genomes YP_763964.1 probable glutamate:gamma-aminobutyric acid transporter YP_763967.1 acetyl-CoA carboxylase biotin holoenzyme synthetase YP_763968.1 lysine translase YP_763969.1 conserved in Francisella genomes YP_763972.1 catalyzes the formation of 3-dehydroshikimate from 3-dehydroquinate in chorismate biosynthesis YP_763973.1 hydrolyzes P(1),P(4)-bis(5'-adenosyl) tetraphosphate to form 2 ADP YP_763974.1 catalyzes the transfer of a total of four methyl groups from S-adenosyl-l-methionine (S-AdoMet) to two adjacent adenosine bases A1518 and A1519 in 16S rRNA; mutations in ksgA causes resistance to the translation initiation inhibitor kasugamycin YP_763975.1 cyclophilin YP_763977.1 catalyzes a two-step reaction, first charging an arginine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA synthetase YP_763979.1 L-asparaginase YP_763981.1 catalyzes the formation of porphobilinogen from 5-aminolevulinate YP_763983.1 DNA nucleotidyltransferase (DNA-directed) YP_763984.1 SohB; periplasmic protein; member of the peptidase S49 family YP_763988.1 amylophosphorylase YP_763989.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_763993.1 D-glutamate ligase YP_763994.1 First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan YP_763995.1 PEP carboxykinase; PEP carboxylase; PEPCK; catalyzes the phosphorylation and decarboxylation of oxaloacetate to form phosphoenolpyruvate using ATP YP_763996.1 catalyzes a two-step reaction, first charging a glutamine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA YP_764002.1 conserved in Francisella genomes YP_764003.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs.; ISFtu1 YP_764007.1 Involved in ubiquinone biosynthesis YP_764008.1 conserved in Francisella genomes YP_764011.1 conserved in Francisella genomes YP_764012.1 ATP:glycerol 3-phosphotransferase YP_764014.1 conserved in Francisella genomes YP_764018.1 conserved in Francisella genomes YP_764019.1 stimulates the release of release factors 1 and 2 from the ribosome after hydrolysis of the ester bond in peptidyl-tRNA has occurred; GDP/GTP-binding protein YP_764020.1 catalyzes the reversible phosphoryl transfer from adenosine triphosphate (ATP) to thymidine monophosphate (dTMP) to form thymidine diphosphate (dTDP) YP_764023.1 deoxyriboaldolase YP_764024.1 catalyzes the transfer of phosphate between the C1 and C5 carbons of pentose YP_764025.1 type III; catalyzes the formation of (R)-4'-phosphopantothenate from (R)-pantothenate in coenzyme A biosynthesis; type III pantothenate kinases are not subject to feedback inhibition from coenzyme A and have a high Km for ATP YP_764026.1 DNA nucleotidyltransferase (DNA-directed) YP_764027.1 transfers the gamma-phosphate of ATP to the 4' position of a tetraacyldisaccharide 1-phosphate intermediate to form tetraacyldisaccharide 1,4'-bis-phosphate YP_764029.1 catalyzes the addition and repair of the 3'-terminal CCA sequence in tRNA; these proteins belong to the CCA-adding enzyme subfamily 2 which does not have phosphohydrolase activity YP_764035.1 possible microcin C7 resistance protein YP_764036.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_764038.1 catalyzes a two-step reaction; charges a cysteine by linking its carboxyl group to the alpha-phosphate of ATP then transfers the aminoacyl-adenylate to its tRNA YP_764039.1 involved in methylation of ribosomal protein L3 YP_764042.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_764043.1 conserved in Francisella genomes YP_764045.1 conserved in Francisella genomes YP_764046.1 conserved in Francisella genomes YP_764047.1 conserved in Francisella genomes YP_764053.1 type II fructose 1,6-bisphosphatae; in Escherichia coli this protein forms a dimer and binds manganese YP_764059.1 conserved in Francisella genomes YP_764061.1 conserved in Francisella genomes YP_764063.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_764064.1 60 kDa chaperone family; promotes refolding of misfolded polypeptides especially under stressful conditions; forms two stacked rings of heptamers to form a barrel-shaped 14mer; ends can be capped by GroES; misfolded proteins enter the barrel where they are refolded when GroES binds; many bacteria have multiple copies of the groEL gene which are active under different environmental conditions; the B.japonicum protein in this cluster is expressed constitutively; in Rhodobacter, Corynebacterium and Rhizobium this protein is essential for growth YP_764065.1 10 kDa chaperonin; Cpn10; GroES; forms homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring YP_764070.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_764073.1 recognizes the termination signals UAG and UAA during protein translation a specificity which is dependent on amino acid residues residing in loops of the L-shaped tRNA-like molecule of RF1; this protein is similar to release factor 2 YP_764074.1 catalyzes the formation of glutamate-1-semialdehyde from glutamyl-tRNA(Glu) and NADPH; the second step of the pathway is catalyzed by glutamate-1-semialdehyde aminomutase which results in the formation of 5-aminolevulinic acid; functions in porphyrin (tetrapyrroles) biosynthesis; the crystal structure showed a C-terminal dimerization domain that appears to be absent in Chlamydial proteins YP_764078.1 decatenates newly replicated chromosomal DNA and relaxes positive and negative DNA supercoiling YP_764081.1 hydrolyzes diadenosine polyphosphate YP_764084.1 acid phosphomonoesterase YP_764087.1 this protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site YP_764088.1 methylates guanosine-37 in various tRNAs; uses S-adenosyl-L-methionine to transfer methyl group to tRNA YP_764089.1 Essential for efficient processing of 16S rRNA YP_764090.1 binds to lower part of 30S body where it stabilizes two domains; required for efficient assembly of 30S; in Escherichia coli this protein has nuclease activity YP_764091.1 catalyzes the formation of S-adenosylmethionine from methionine and ATP; methionine adenosyltransferase YP_764093.1 in most organisms, only the N-terminal domain is present in a single polypeptide; in some archaea this domain is fused to a kinase domain; this gene is essential for growth in Escherichia coli and Bacillus subtilis; the secreted glycoprotease from Pasteurella haemolytica showed specificity for O-sialoglycosylated proteins; the Pyrococcus structure shows DNA-binding properties, iron-binding, ATP-binding, and AP endonuclease activity YP_764094.1 conserved in Francisella genomes YP_764095.1 DNA-dependent RNA polymerase YP_764096.1 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates; beta subunit is part of the catalytic core which binds with a sigma factor to produce the holoenzyme YP_764098.1 present in two forms; L12 is normal, while L7 is aminoacylated at the N-terminal serine; the only multicopy ribosomal protein; 4:1 ratio of L7/L12 per ribosome; two L12 dimers bind L10; critically important for translation efficiency and fidelity; stimulates GTPase activity of translation factors YP_764099.1 binds the two ribosomal protein L7/L12 dimers and anchors them to the large ribosomal subunit YP_764100.2 in Escherichia coli and Methanococcus, this protein autoregulates expression; the binding site in the mRNA mimics the binding site in the 23S rRNA YP_764101.1 binds directly to 23S ribosomal RNA YP_764103.1 EF-Tu; promotes GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis; when the tRNA anticodon matches the mRNA codon, GTP hydrolysis results; the inactive EF-Tu-GDP leaves the ribosome and release of GDP is promoted by elongation factor Ts; many prokaryotes have two copies of the gene encoding EF-Tu YP_764105.1 conserved in Francisella genomes YP_764112.1 conserved in Francisella genomes YP_764113.1 Catalyzes the conversion of citrate to isocitrate YP_764116.1 coprogen oxidase YP_764121.1 Reversibly isomerizes the ketone sugar dihydroxyacetone phosphate to the aldehyde sugar glyceraldehyde-3-phosphate YP_764122.1 catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate YP_764123.1 catalyzes a salvage reaction resulting in the formation of AMP which is metabolically less costly than a de novo synthesis YP_764124.1 dihydrolipoamide S-succinyltransferase YP_764125.1 SucA; E1 component of the oxoglutarate dehydrogenase complex which catalyzes the formation of succinyl-CoA from 2-oxoglutarate; SucA catalyzes the reaction of 2-oxoglutarate with dihydrolipoamide succinyltransferase-lipoate to form dihydrolipoamide succinyltransferase-succinyldihydrolipoate and carbon dioxide YP_764126.1 part of four member succinate dehydrogenase enzyme complex that forms a trimeric complex (trimer of tetramers); SdhA/B are the catalytic subcomplex and can exhibit succinate dehydrogenase activity in the absence of SdhC/D which are the membrane components and form cytochrome b556; SdhC binds ubiquinone; oxidizes succinate to fumarate while reducing ubiquinone to ubiquinol; the catalytic subunits are similar to fumarate reductase YP_764127.1 fumarate dehydrogenase YP_764128.1 fumarate dehydrogenase YP_764129.1 fumarate dehydrogenase YP_764130.1 (R)-citric synthase YP_764131.1 AmpG-signal transducer YP_764132.1 conserved in Francisella genomes YP_764136.1 produces ATP from ADP in the presence of a proton gradient across the membrane; the epsilon subunit is part of the catalytic core of the ATP synthase complex YP_764137.1 Produces ATP from ADP in the presence of a proton gradient across the membrane. The beta chain is a regulatory subunit YP_764138.1 produces ATP from ADP in the presence of a proton gradient across the membrane; the gamma chain is a regulatory subunit YP_764139.1 produces ATP from ADP in the presence of a proton gradient across the membrane; the alpha chain is a catalytic subunit YP_764140.1 Produces ATP from ADP in the presence of a proton gradient across the membrane; the delta subunit is part of the catalytic core of the ATP synthase complex YP_764141.1 Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit B is part of the membrane proton channel. YP_764142.1 produces ATP from ADP in the presence of a proton gradient across the membrane; subunit C is part of the membrane proton channel F0 YP_764143.1 Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit A is part of the membrane proton channel F0 YP_764144.1 conserved in Francisella genomes YP_764146.1 pseudouridine synthase YP_764149.1 catalyzes a two-step reaction, first charging a histidine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class II aminoacyl-tRNA synthetase; forms homodimers; some organisms have a paralogous gene, hisZ, that is similar to hisS and produces a protein that performs the first step in histidine biosynthesis along with HisG YP_764151.1 Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits during initiation of protein synthesis. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex YP_764152.1 modifies transcription through interactions with RNA polymerase affecting elongation, readthrough, termination, and antitermination YP_764154.1 catalyzes the formation of coproporphyrinogen from uroporphyrinogen III YP_764155.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_764156.1 conserved in Francisella genomes YP_764157.1 coenzyme Q reductase YP_764158.1 coenzyme Q reductase YP_764159.1 coenzyme Q reductase YP_764160.1 coenzyme Q reductase YP_764161.1 coenzyme Q reductase YP_764162.1 Catalyzes the transfer of electrons from NADH to quinone YP_764163.1 coenzyme Q reductase YP_764164.1 Catalyzes the transfer of electrons from NADH to ubiquinone YP_764165.1 coenzyme Q reductase YP_764166.1 coenzyme Q reductase YP_764167.1 Catalyzes the transfer of electrons from NADH to quinone YP_764168.1 coenzyme Q reductase YP_764169.1 The point of entry for the majority of electrons that traverse the respiratory chain eventually resulting in the reduction of oxygen YP_764174.1 DAP decarboxylase YP_764176.1 conserved in Francisella genomes YP_764177.1 conserved in Francisella genomes YP_764179.1 conserved in Francisella genomes YP_764180.1 allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA YP_764181.1 allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA YP_764183.1 conserved in Francisella genomes YP_764184.1 Catalyzes two discrete reactions in the de novo synthesis of purines: the cleavage of adenylosuccinate and succinylaminoimidazole carboxamide ribotide YP_764185.1 conserved in Francisella genomes YP_764186.1 conserved in Francisella genomes YP_764188.1 catalyzes the formation of 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and L-glutamine in purine biosynthesis YP_764189.1 Catalyzes first step of the de novo purine nucleotide biosynthetic pathway YP_764192.1 L-isoaspartyl protein carboxyl methyltransferase YP_764197.1 essential GTPase; exhibits high exchange rate for GTP/GDP; associates with 50S ribosomal subunit; involved in regulation of chromosomal replication YP_764199.1 conserved in Francisella genomes YP_764200.1 K(+)-importing ATPase YP_764201.1 catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions YP_764202.1 catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate in leucine biosynthesis; forms a heterodimer of LeuC/D YP_764203.1 dehydratase component, catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate YP_764204.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_764205.1 conserved in Francisella genomes YP_764208.1 DNA nucleotidyltransferase (DNA-directed) YP_764210.1 L-glutamine amidohydrolase YP_764211.1 conserved in Francisella genomes YP_764212.1 lysine translase YP_764215.1 DNA nucleotidyltransferase (DNA-directed) YP_764216.1 zinc-dependent; catalyzes the deacetylation of UDP-(3-O-acyl)-N-acetylglucosamine to UDP-3-O-(3-hydroxytetradecanoyl)-glucosamine in the second step of lipid A biosynthesis YP_764217.1 GTPase; similar structure to tubulin; forms ring-shaped polymers at the site of cell division; other proteins such as FtsA, ZipA, and ZapA, interact with and regulate FtsZ function YP_764220.1 D-alanine--D-alanine ligase; DdlA; DdlB; cytoplasmic; catalyzes the formation of D-alanyl-D-alanine from two D-alanines in peptidoglycan synthesis; there are two forms of this enzyme in Escherichia coli YP_764222.1 in Escherichia coli this protein is involved in binding to the leader sequence of mRNAs and is itself bound to the 30S subunit; autoregulates expression via a C-terminal domain; in most gram negative organisms this protein is composed of 6 repeats of the S1 domain while in gram positive there are 4 repeats; the S1 nucleic acid-binding domain is found associated with other proteins YP_764230.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_764231.1 involved in de novo purine biosynthesis YP_764232.1 catalyzes the formation of N6-(1,2,-dicarboxyethyl)-AMP from L-aspartate, inosine monophosphate and GTP in AMP biosynthesis YP_764233.1 guanine phosphoribosyltransferase YP_764241.1 replication factor Y YP_764242.1 ISFtu1 transposase. Member of the IS630 Tc-1 mariner family. A programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3', is located 325 nt from the 5' end (a.a. 109) which enables frameshifting to produce a single ORF (this is a regulatory mechanism associated with some transposases). Alignment of the frameshifted ORF with additional members of the IS630 family, and mariner elements, shows that this transposase shares conserved residues, including a fully conserved, Asp-Glu (DE) dipeptide, and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if the ribosomal frameshift occurs. YP_764243.1 ChvD; in Agrobacterium tumefaciens, mutations in both Walker boxes were found to affect virulence YP_764245.1 conserved in Francisella genomes YP_764246.1 conserved in Francisella genomes YP_764248.1 amino-oligopeptidase YP_764250.1 monomeric bifunctional protein; functions in tryptophan biosynthesis pathway; phosphoribosylanthranilate is rearranged to carboxyphenylaminodeoxyribulosephosphate which is then closed to form indole-3-glycerol phosphate YP_764251.1 peptide Met(O) reductase YP_764254.1 conserved in Francisella genomes YP_764255.1 Catalyzes the conversion of N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate YP_764256.1 glutamine amido-transferase YP_764257.1 with component II, the glutamine amidotransferase, catalyzes the formation of anthranilate from chorismate and glutamine