-- dump date 20140619_105401 -- class Genbank::Contig -- table contig_comment -- id comment NC_010519.1 PROVISIONAL REFSEQ: This record has not yet been subject to finalPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.govPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu)PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu)PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org)PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-StanfordPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANLPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University HealthPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC)PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initialPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achievedPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National LaboratoryPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled withPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled with parallel phrap (High Performance Software, LLC). PossiblePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with Dupfinisher (unpublished, C.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with Dupfinisher (unpublished, C. Han) or transposon bomb of bridging clones (EZ-Tn5 PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with Dupfinisher (unpublished, C. Han) or transposon bomb of bridging clones (EZ-Tn5 Tnp transposome kit, Epicentre Biotechnologies). Gaps betweenPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with Dupfinisher (unpublished, C. Han) or transposon bomb of bridging clones (EZ-Tn5 Tnp transposome kit, Epicentre Biotechnologies). Gaps between contigs were closed by editing, custom primer walking, or PCRPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with Dupfinisher (unpublished, C. Han) or transposon bomb of bridging clones (EZ-Tn5 Tnp transposome kit, Epicentre Biotechnologies). Gaps between contigs were closed by editing, custom primer walking, or PCR amplification. The completed genome sequence of H. somnus strainPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with Dupfinisher (unpublished, C. Han) or transposon bomb of bridging clones (EZ-Tn5 Tnp transposome kit, Epicentre Biotechnologies). Gaps between contigs were closed by editing, custom primer walking, or PCR amplification. The completed genome sequence of H. somnus strain 2336 contains 26472 reads, achieving an average of 9.7-foldPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with Dupfinisher (unpublished, C. Han) or transposon bomb of bridging clones (EZ-Tn5 Tnp transposome kit, Epicentre Biotechnologies). Gaps between contigs were closed by editing, custom primer walking, or PCR amplification. The completed genome sequence of H. somnus strain 2336 contains 26472 reads, achieving an average of 9.7-fold sequence coverage per base with an error rate less than 1 inPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with Dupfinisher (unpublished, C. Han) or transposon bomb of bridging clones (EZ-Tn5 Tnp transposome kit, Epicentre Biotechnologies). Gaps between contigs were closed by editing, custom primer walking, or PCR amplification. The completed genome sequence of H. somnus strain 2336 contains 26472 reads, achieving an average of 9.7-fold sequence coverage per base with an error rate less than 1 in 100,000.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with Dupfinisher (unpublished, C. Han) or transposon bomb of bridging clones (EZ-Tn5 Tnp transposome kit, Epicentre Biotechnologies). Gaps between contigs were closed by editing, custom primer walking, or PCR amplification. The completed genome sequence of H. somnus strain 2336 contains 26472 reads, achieving an average of 9.7-fold sequence coverage per base with an error rate less than 1 in 100,000. The JGI and collaborators endorse the principles for thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with Dupfinisher (unpublished, C. Han) or transposon bomb of bridging clones (EZ-Tn5 Tnp transposome kit, Epicentre Biotechnologies). Gaps between contigs were closed by editing, custom primer walking, or PCR amplification. The completed genome sequence of H. somnus strain 2336 contains 26472 reads, achieving an average of 9.7-fold sequence coverage per base with an error rate less than 1 in 100,000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with Dupfinisher (unpublished, C. Han) or transposon bomb of bridging clones (EZ-Tn5 Tnp transposome kit, Epicentre Biotechnologies). Gaps between contigs were closed by editing, custom primer walking, or PCR amplification. The completed genome sequence of H. somnus strain 2336 contains 26472 reads, achieving an average of 9.7-fold sequence coverage per base with an error rate less than 1 in 100,000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data toPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with Dupfinisher (unpublished, C. Han) or transposon bomb of bridging clones (EZ-Tn5 Tnp transposome kit, Epicentre Biotechnologies). Gaps between contigs were closed by editing, custom primer walking, or PCR amplification. The completed genome sequence of H. somnus strain 2336 contains 26472 reads, achieving an average of 9.7-fold sequence coverage per base with an error rate less than 1 in 100,000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of thisPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with Dupfinisher (unpublished, C. Han) or transposon bomb of bridging clones (EZ-Tn5 Tnp transposome kit, Epicentre Biotechnologies). Gaps between contigs were closed by editing, custom primer walking, or PCR amplification. The completed genome sequence of H. somnus strain 2336 contains 26472 reads, achieving an average of 9.7-fold sequence coverage per base with an error rate less than 1 in 100,000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborativePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with Dupfinisher (unpublished, C. Han) or transposon bomb of bridging clones (EZ-Tn5 Tnp transposome kit, Epicentre Biotechnologies). Gaps between contigs were closed by editing, custom primer walking, or PCR amplification. The completed genome sequence of H. somnus strain 2336 contains 26472 reads, achieving an average of 9.7-fold sequence coverage per base with an error rate less than 1 in 100,000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with Dupfinisher (unpublished, C. Han) or transposon bomb of bridging clones (EZ-Tn5 Tnp transposome kit, Epicentre Biotechnologies). Gaps between contigs were closed by editing, custom primer walking, or PCR amplification. The completed genome sequence of H. somnus strain 2336 contains 26472 reads, achieving an average of 9.7-fold sequence coverage per base with an error rate less than 1 in 100,000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376).PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000947. URL -- http://www.jgi.doe.gov JGI Project ID: 4042873 Source DNA and bacteria available from Tom Inzana (tinzana@vt.edu) Contacts: Tom Inzana (tinzana@vt.edu) Paul Richardson (microbes@cuba.jgi-psf.org) Quality assurance done by JGI-Stanford Annotation done by JGI-ORNL and JGI-LANL Draft sequencing was performed at the Oklahoma University Health Sciences Center (OUHSC) http://microgen.ouhsc.edu/h_somnus/h_somnus_home.htm) and initial gap closure was done at Virginia Tech. The finishing was achieved at the Joint Genome Institute's Los Alamos National Laboratory (LANL). During final finishing at LANL, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with Dupfinisher (unpublished, C. Han) or transposon bomb of bridging clones (EZ-Tn5 Tnp transposome kit, Epicentre Biotechnologies). Gaps between contigs were closed by editing, custom primer walking, or PCR amplification. The completed genome sequence of H. somnus strain 2336 contains 26472 reads, achieving an average of 9.7-fold sequence coverage per base with an error rate less than 1 in 100,000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). COMPLETENESS: full length.