-- dump date 20140619_155355 -- class Genbank::Feature -- table feature -- table main -- field 1 id -- field 2 type -- field 3 name -- field 4 contig -- field 5 start_pos -- field 6 end_pos -- field 7 strand -- field 8 description -- field 9 chrom_position -- field 10 organism -- field 11 GeneID -- header -- id type name contig start_pos end_pos strand description chrom_position organism GeneID 362242000001 SEQ_END SEQ_END NC_005916.1 174155 174155 DR NC_005916.1; contig end 174155..174155 Mycobacterium ulcerans Agy99 362242000002 SEQ_END SEQ_END NC_008611.1 5631606 5631606 DR NC_008611.1; contig end 5631606..5631606 Mycobacterium ulcerans Agy99 YP_025523.1 CDS rep NC_005916.1 1 1107 D MUP001, rep, len: 368 aa. Probable rep, similar to the Rep proteins on several Mycobacterial plasmids e.g. O32860 Rep of the plasmid pJAZ38 from Mycobacterium fortuitum (see Gavigan et al., 1997) (368 aa), fasta scores: opt: 1692, E(): 1.7e-96, (68.306% identity in 366 aa overlap); and Q938A1 RepA of the plasmid pVT2 from Mycobacterium avium (391 aa), fasta scores: opt: 1283, E(): 2.6e-71, (55.556% identity in 360 aa overlap); and Q48888 Rep of the plasmid pLR7 from Mycobacterium avium (360 aa). Also weakly similar to Q9ZHK2 Putative replication protein of the plasmid pSOX from Rhodococcus sp. X309 (368 aa), fasta scores: opt: 272, E(): 4.1e-09, (36.022% identity in 372 aa overlap). Contains Prosite match to entry PS50310 Alanine-rich region.; replication protein Rep 1..1107 Mycobacterium ulcerans Agy99 2846617 YP_025524.1 CDS MUP002c NC_005916.1 1117 1431 R MUP002c, -, len: 104 aa. Hypothetical protein.; hypothetical protein complement(1117..1431) Mycobacterium ulcerans Agy99 2846606 YP_025525.1 CDS MUP003 NC_005916.1 1694 2290 D MUP003, -, len: 198 aa. Hypothetical protein. Contains a helix turn helix motif between aa 30->51, Standard_deviations: 4.86, score 1665.00.; hypothetical protein 1694..2290 Mycobacterium ulcerans Agy99 2846561 YP_025526.1 CDS MUP004c NC_005916.1 2310 2924 R MUP004c, -, len: 204 aa. Hypothetical protein. An alternative start site exists at nt 2588.; hypothetical protein complement(2310..2924) Mycobacterium ulcerans Agy99 2846562 YP_025527.1 CDS parA NC_005916.1 2921 3901 R MUP005c, parA, len: 326 aa. Possible parA, chromosome partitioning protein, similar to several e.g. O05189|PARA_CAUCR Chromosome partitioning protein parA from Caulobacter crescentus (267 aa), fasta scores: opt: 279, E(): 4.3e-10, (29.918% identity in 244 aa overlap); Q8G6J3|AAN24469 Chromosome partitioning protein P from Bifidobacterium longum (336 aa), fasta scores: opt: 324, E(): 7.3e-13, (30.085% identity in 236 aa overlap) and Q8KBD6 ATPase, ParA family from Chlorobium tepidum (265 aa), fasta scores: opt: 285, E(): 1.8e-10, (28.807% identity in 243 aa overlap). Contains a Pfam match to entry PF00991, ParA family ATPase.; chromosome partitioning protein ParA complement(2921..3901) Mycobacterium ulcerans Agy99 2846551 YP_025528.1 CDS MUP006c NC_005916.1 5640 6386 R MUP006c, -, len: 248 aa. Hypothetical protein.; hypothetical protein complement(5640..6386) Mycobacterium ulcerans Agy99 2846563 YP_025529.1 CDS MUP007c NC_005916.1 6383 6604 R MUP007c, -, len: 73 aa. Conserved hypothetical protein, some similarity to O33270|Rv0340 Hypothetical protein from M. tuberculosis (179 aa), fasta scores: opt: 88, E(): 21, (32.558% identity in 43 aa overlap); and Q8KKW1 Hypothetical protein on the symbiotic plasmid p42d from Rhizobium etli (108 aa), fasta scores: opt: 92, E(): 7.1, (58.333% identity in 24 aa overlap); and O52677 Core protein (Fragment) from Escherichia coli (193 aa), fasta scores: opt: 91, E(): 13, (31.818% identity in 66 aa overlap).; hypothetical protein complement(6383..6604) Mycobacterium ulcerans Agy99 2846564 YP_025530.1 CDS MUP008c NC_005916.1 6612 7160 R MUP008c, -, len: 182 aa. Possible nucleic acid binding protein, similar to the C-terminus of several N utilization substance proteins e.g. O05818|NUSA_MYCTU|Rv2841c N utilization substance protein A from M. tuberculosis (347 aa), fasta scores: opt: 337, E(): 6.5e-14, (42.143% identity in 140 aa overlap); Q9HV54 N utilization substance protein A from Pseudomonas aeruginosa (493 aa), fasta scores: opt: 353, E(): 7.9e-15, (38.255% identity in 149 aa overlap); etc. Contains a possible Pfam match to entry PF00013 KH domain.; nucleic acid binding protein complement(6612..7160) Mycobacterium ulcerans Agy99 2846618 YP_025531.1 CDS MUP009 NC_005916.1 7188 7616 D MUP009, -, 142 aa. Hypothetical protein.; hypothetical protein 7188..7616 Mycobacterium ulcerans Agy99 2846574 YP_025532.1 CDS MUP010 NC_005916.1 7630 8421 D MUP010, -, len: 263 aa. Hypothetical protein.; hypothetical protein 7630..8421 Mycobacterium ulcerans Agy99 2846575 YP_025533.1 CDS pknQ NC_005916.1 8430 10412 D MUP011, pknQ, len: 660 aa. Probable pknQ, transmembrane serine/threonine-protein kinase (EC 2.7.1.-), similar to several mycobacterial serine/threonine-protein kinases e.g. Q10697|PKNJ_MYCTU|Rv2088 from M. tuberculosis (589 aa), fasta scores: opt: 1121, E(): 2.9e-43, (43.45% identity in 527 aa overlap); P72003|PKNF_MYCTU|Rv1746 from M. tuberculosis (476 aa), fasta scores: opt: 761, E(): 4.2e-27, (38.661% identity in 463 aa overlap); Q9RLQ7 Putative serine/threonine protein kinase from M. bovis (291 aa), etc. And similar to serine/threonine-protein kinases from other bacteria e.g. Q9XA16 Putative serine/threonine protein kinase from Streptomyces coelicolor (673 aa), fasta scores: opt: 656, E(): 2.9e-22 (36.164% identity in 365 aa overlap). Contains a Prosite matches to entries PS50011 Protein kinase domain and PS00108, Serine/Threonine protein kinases active-site signature pattern. Belongs to the SER/THR family of protein kinases.; transmembrane serine/threonine-protein kinase PknQ 8430..10412 Mycobacterium ulcerans Agy99 2846552 YP_025534.1 CDS MUP012c NC_005916.1 10429 10692 R MUP012c, -, len: 87 aa. Hypothetical protein. Some similarity to Antigen 84 from M. tuberculosis and M. leprae e.g. P46816|AG84_MYCTU|Rv2145c Antigen 84 from M. tuberculosis (260 aa), fasta scores: opt: 105, E(): 4.1, (36.735% identity in 49 aa overlap).; hypothetical protein complement(10429..10692) Mycobacterium ulcerans Agy99 2846576 YP_025535.1 CDS MUP013c NC_005916.1 10689 11147 R MUP013c, -, len: 152 aa. Possible conserved membrane protein, some similarity to Q8EX03 Putative integral membrane protein from Mycoplasma penetrans (303 aa), fasta scores: opt: 106, E(): 14, (31.250% identity in 96 aa overlap). Contains two membrane spanning regions towards the N-terminus.; hypothetical protein complement(10689..11147) Mycobacterium ulcerans Agy99 2846612 YP_025536.1 CDS MUP014c NC_005916.1 11149 11922 R MUP014c, -, len: 257 aa. Putative integral membrane protein, contains several membrane spanning regions. An alternative start codon exists downstream.; putative integral membrane protein complement(11149..11922) Mycobacterium ulcerans Agy99 2846613 YP_025537.1 CDS MUP015c NC_005916.1 11916 12692 R MUP015c, -, len: 258 aa. Possible secreted protein, contains probable N-terminal signal sequence.; hypothetical protein complement(11916..12692) Mycobacterium ulcerans Agy99 2846614 YP_025538.1 CDS MUP016c NC_005916.1 12689 13480 R MUP016c, -, len: 263 aa. Hypothetical protein. Contains a Prosite match to entry PS00152 ATPASE_ALPHA_BETA ATP synthase alpha and beta subunits signature; hypothetical protein complement(12689..13480) Mycobacterium ulcerans Agy99 2846615 YP_025539.1 CDS MUP017c NC_005916.1 13477 13929 R MUP017c, -, len: 150 aa. Possible conserved transmembrane protein, weakly similar to O06254|Rv3437 possible conserved transmembrane protein from M. tuberculosis (158 aa), fasta scores: opt: 244, E(): 2.3e-05, (38.393% identity in 112 aa overlap). Contains a Prosite match to entry PS50099 PRO_RICH Proline-rich region. Contains hydrophobic region at the C-terminus.; transmembrane protein complement(13477..13929) Mycobacterium ulcerans Agy99 2846616 YP_025540.1 CDS MUP018c NC_005916.1 13973 15061 R MUP018c, -, len: 362 aa. Probable forkhead-associated protein, similar to several other M. tuberculosis conserved hypothetical ala-rich proteins e.g. P96214|Rv3863 Hypothetical protein (392 aa), fasta scores: opt: 525, E(): 1.9e-25, (33.602% identity in 372 aa overlap); O50389|Rv3360 Hypothetical protein (122 aa), fasta scores: opt: 198, E(): 3.4e-05, (37.113% identity in 97 aa overlap); and similar to the N-terminus of O65934|Rv1747 hypothetical 92.2 kDa protein (ABC transporter, ATP-binding protein (865 aa), fasta scores: opt: 207, E(): 4.3e-05, (37.255% identity in 102 aa overlap). Contains a Prosite match to entries PS50006 FHA_DOMAIN Forkhead-associated (FHA) domain and PS50310 ALA_RICH Alanine-rich region. And a Pfam match to entry PF01381 HTH_3, Helix-turn-helix.; forkhead-associated protein complement(13973..15061) Mycobacterium ulcerans Agy99 2846607 YP_025541.1 CDS MUP019 NC_005916.1 15406 16440 D MUP019, -, len: 344 aa. Probable conserved membrane protein, weakly similar to several membrane associated proteins from M. tuberculosis e.g. to the C-terminus of two probable serine/threonine-protein kinases Q11053|PKNH_MYCTU|Rv1266c (626 aa), fasta scores: opt: 483, E(): 3.7e-16, (29.032% identity in 341 aa overlap); and PKNJ_MYCTU|Q10697|Rv2088 (589 aa), fasta scores: opt: 315, E(): 5.4e-08, (25.649% identity in 308 aa overlap). Also weakly similar to P96858|Rv3576 Possible lipoprotein lppH from M. tuberculosis (237 aa), fasta scores: opt: 380, E(): 1.9e-11, (29.918% identity in 244 aa overlap). Contains a Prosite match to entry PS50099 PRO_RICH Proline-rich region. Contains a membrane spanning region between aa 102 to 124.; hypothetical protein 15406..16440 Mycobacterium ulcerans Agy99 2846608 YP_025542.1 CDS MUP020 NC_005916.1 16430 16612 D MUP020, -, len: 60 aa. Conserved hypothetical protein, some similarity to Q983E1 Hypothetical protein mll8367 from Rhizobium loti (Mesorhizobium loti) (91 aa), fasta scores: opt: 92, E(): 4.5, (35.00% identity in 40 aa overlap).; hypothetical protein 16430..16612 Mycobacterium ulcerans Agy99 2846609 YP_025543.1 CDS MUP021 NC_005916.1 16609 16872 D MUP021, -, len: 87 aa. Possible transcriptional regulatory protein, similar to other possible WhiB-like regulatory proteins e.g. O69649|Rv3681c Probable whiB4 from M. tuberculosis (118 aa), fasta scores: opt: 141, E(): 0.001, (44.681% identity in 47 aa overlap); P96215|Rv3862c Possible whiB6 from M. tuberculosis (116 aa), fasta scores: opt: 133, E(): 0.0054, (29.412% identity in 85 aa overlap). Also weakly similar to other putative regulatory proteins e.g. Q9S2B9 Putative regulatory protein from Streptomyces coelicolor (112 aa), fasta scores: opt: 115, E(): 0.11, (34.211% identity in 76 aa overlap). Contains a Pfam match to entry PF02467 Transcription factor WhiB.; transcriptional regulatory protein 16609..16872 Mycobacterium ulcerans Agy99 2846610 YP_025544.1 CDS MUP022 NC_005916.1 17287 18621 D MUP022, -, len: 444 aa. Probable transposase for IS2606, highly similar to Q9ZG97 Probable transposase from M. ulcerans (444 aa), fasta scores: opt: 2409, E(): 5.3e-154, (97.872% identity in 376 aa overlap); and similar to O05912 Hypothetical 48.7 kDa protein (IS1554, transposase) from M. tuberculosis (439 aa), fasta scores: opt: 1675, E(): 1.1e-104, (68.564% identity in 369 aa overlap).; transposase for the insertion element IS2606 17287..18621 Mycobacterium ulcerans Agy99 2846611 YP_025545.1 CDS MUP023c NC_005916.1 18772 19404 R MUP023c, -, len: 210 aa. Hypothetical protein.; hypothetical protein complement(18772..19404) Mycobacterium ulcerans Agy99 2846553 YP_025546.1 CDS MUP024c NC_005916.1 19401 19988 R MUP024c, -, len: 195 aa. Hypothetical protein.; hypothetical protein complement(19401..19988) Mycobacterium ulcerans Agy99 2846554 YP_025547.1 CDS MUP025 NC_005916.1 20718 22457 D MUP025, -, len: 579 aa. Putative transposase, weakly similar to several putative transposases e.g. Q8R6J9 Transposase from Thermoanaerobacter tengcongensis (565 aa), fasta scores: opt: 315, E(): 5.2e-13, (22.095% identity in 525 aa overlap); O70063 Putative transposase from Mycobacterium smegmatis (503 aa), fasta scores: opt: 221, E(): 9.3e-07, (26.190% identity in 504 aa overlap); Q9Z681 Transposase from Mycoplasma mycoides subsp. mycoides SC (483 aa), fasta scores: opt: 221, E(): 9e-07, (23.288% identity in 365 aa overlap). Almost identical to MUP028 and highly similar to MUP037 but longer.; putative transposase 20718..22457 Mycobacterium ulcerans Agy99 2846555 YP_025548.1 CDS MUP026 NC_005916.1 22629 23963 D MUP026, -, len: 444 aa. Probable transposase for IS2606, highly similar to Q9ZG97 Probable transposase from M. ulcerans (444 aa), fasta scores: opt: 2874, E(): 3.8e-192, (98.423% identity in 444 aa overlap); and similar to O05912 Hypothetical 48.7 kDa protein (IS1554, transposase) from M. tuberculosis (439 aa), fasta scores: opt: 2063, E(): 1.1e-135, (71.034% identity in 435 aa overlap).; transposase for the insertion element IS2606 22629..23963 Mycobacterium ulcerans Agy99 2846556 YP_025549.1 CDS MUP027c NC_005916.1 24162 24980 R MUP027c, -, len: 272 aa. Putative transposase, similar to several putative transposases e.g. P71924|Rv2424c Probable transposase for IS1558 from Mycobacterium tuberculosis (333 aa), fasta scores: opt: 755, E(): 1.7e-42, (45.118% identity in 297 aa overlap); Q8RAU1 Transposase from Thermoanaerobacter tengcongensis (405 aa), fasta scores: opt: 756, E(): 1.7e-42, (42.517% identity in 294 aa overlap). Contains a Pfam match to entry PF02371, Transposase IS116/IS110/IS902 family.; putative transposase complement(24162..24980) Mycobacterium ulcerans Agy99 2846538 YP_025550.1 CDS MUP028c NC_005916.1 25197 26936 R MUP028c, -, len: 579 aa. Putative transposase, weakly similar to several putative transposases e.g. Q8R6J9 Transposase from Thermoanaerobacter tengcongensis (565 aa), fasta scores: opt: 315, E(): 6.8e-13, (22.095% identity in 525 aa overlap); O70063 Putative transposase from Mycobacterium smegmatis (503 aa), fasta scores: opt: 221, E(): 1.1e-06, (26.190% identity in 504 aa overlap); Q9Z681 Transposase from Mycoplasma mycoides subsp. mycoides SC (483 aa), fasta scores: opt: 221, E(): 1.1e-06, (23.288% identity in 365 aa overlap). Almost identical to MUP025 and highly similar to MUP037 but longer.; putative transposase complement(25197..26936) Mycobacterium ulcerans Agy99 2846539 YP_025551.1 CDS MUP029c NC_005916.1 26980 27321 R MUP029c, -, len: 113 aa. Probable IS2404 transposase fragment, highly similar to the C-terminus of O07439 Putative transposase from Mycobacterium ulcerans (327 aa), fasta scores: opt: 496, E(): 3.7e-31, (94.805% identity in 77 aa overlap). Note that this homology extends across the upstream TAG stop codon into the upstream MUP030, suggesting a possible readthrough of the previous stop codon. No sequence errors have been identified and this insertion sequence containing the internal stop codon is repeated at two other locations on the plasmid, MUP047 + MUP048 and MUP062 + MUP063.; transposase for the insertion element IS2404 (fragment) complement(26980..27321) Mycobacterium ulcerans Agy99 2846540 YP_025552.1 CDS MUP030c NC_005916.1 27322 28026 R MUP030c, -, len: 234 aa. Probable IS2404 transposase fragment, highly similar to O07439 Putative transposase from Mycobacterium ulcerans (327 aa), fasta scores: opt: 1839, E(): 2.4e-119, (92.949% identity in 312 aa overlap). Note that this homology extends across the stop codon and directly into MUP029, suggesting a possible readthrough of the TAG stop codon. No sequence errors have been identified and this insertion sequence containing the internal stop codon is repeated at two other locations on the plasmid, MUP047 + MUP048 and MUP062 + MUP063.; transposase for the insertion element IS2404 (fragment) complement(27322..28026) Mycobacterium ulcerans Agy99 2846541 YP_025553.1 CDS MUP031c NC_005916.1 28386 29720 R MUP031c, -, len: 444 aa. Probable transposase for IS2606, highly similar to Q9ZG97 Probable transposase from Mycobacterium ulcerans (444 aa), fasta scores: opt: 2879, E(): 6.2e-188, (98.423% identity in 444 aa overlap); and to O05912 Hypothetical 48.7 kDa protein (IS1554, transposase) from M. tuberculosis (439 aa), fasta scores: opt: 2055, E(): 5e-132, (70.805% identity in 435 aa overlap).; transposase for the insertion element IS2606 complement(28386..29720) Mycobacterium ulcerans Agy99 2846542 YP_025554.1 CDS mlsB NC_005916.1 30054 72446 R MUP032c, mlsB, len: 14130 aa. Type I modular polyketide synthase, composed of a loading module and seven extender modules; required for the synthesis of the mycolactone side-chain in M. ulcerans (see Stinear et al., 2003). Similar to the modules in several other bacterial polyketide synthases e.g. Q93NX9 AmphI from Streptomyces nodosus (9510 aa), fasta scores: opt: 10163, E(): 0, (41.418% identity in 10394 aa overlap); Q9ALM3 Polyketide synthase extender modules 5-7 from Saccharopolyspora spinosa (4928 aa), fasta scores: opt: 8980, E(): 0, (46.330% identity in 4945 aa overlap); Q93HJ2 Modular polyketide synthase from Streptomyces avermitilis (4685 aa), fasta scores: opt: 5558, E(): 0, (44.649% identity in 4934 aa overlap). Contains 8 x Pfam matches to entry PF00109 ketoacyl-synt: Beta-ketoacyl synthase, N-terminal domain and 8 x Pfam matches to entry PF02801 ketoacyl-synt_C: Beta-ketoacyl synthase, C-terminal domain. Contains 8 x Pfam matches to entry PF00550 pp-binding: Phosphopantetheine attachment site and 8 x Pfam matches to entry PF00698 Acyl_transf: Acyl transferase domain.; Type I modular polyketide synthase complement(30054..72446) Mycobacterium ulcerans Agy99 2846550 YP_025555.1 CDS MUP033c NC_005916.1 72536 72910 R MUP033c, -, len: 124 aa. Putative transposase, similar in part to several transposases e.g. BAC67824 Putative transposase from Streptomyces avermitilis (239 aa), fasta scores: opt: 263, E(): 3.7e-12, (50.617% identity in 81 aa overlap); Q8KRD5 ISRh1 transposase-like protein from Rhodococcus erythropolis (236 aa), fasta score: opt: 257, E(): 1e-11, (64.286% identity in 56 aa overlap); Q982I3 Transposase from plasmid pMLa of Rhizobium loti (Mesorhizobium loti) (185 aa), fasta scores: opt: 200, E(): 1.6e-07, (37.209% identity in 86 aa overlap). Highly similar to MUP041.; putative transposase complement(72536..72910) Mycobacterium ulcerans Agy99 2846557 YP_025556.1 CDS MUP034c NC_005916.1 73008 73547 R MUP034c, -, len: 179 aa. Putative transposase, weakly similar to several transposases from Xanthomonas e.g. Q8NL03 ISxac2 transposase from plasmid pXAC33 and pXAC64 of Xanthomonas axonopodis (275 aa), fasta scores: opt: 214, E(): 5.5e-07, (33.766% identity in 146 aa overlap); Q8PCU3 ISxcC1 transposase from Xanthomonas campestris (255 aa), fasta scores: opt: 198, E(): 3.8e-06, (34.286% identity in 105 aa overlap). Also weakly similar to Q9X9H9 IS1400 transposase B from Yersinia enterocolitica (294 aa), fasta scores: opt: 202, E(): 2.8e-06, (38.095% identity in 105 aa overlap). Almost identical to MUP042. Contains a Pfam match to entry PF00665 rve Integrase core domain.; putative transposase complement(73008..73547) Mycobacterium ulcerans Agy99 2846558 YP_025557.1 CDS MUP035 NC_005916.1 74138 74851 D MUP035, -, len: 237 aa. Putative transposase, similar to several transposases from Streptomyces e.g. BAC68344|Q82Q86 Putative transposase from Streptomyces avermitilis (354 aa), fasta scores: opt: 551, E(): 5.1e-23, (52.299% identity in 174 aa overlap); O69923 Putative transposase from Streptomyces coelicolor (379 aa), fasta score: opt: 309, E(): 7.6e-10, (35.897% identity in 195 aa overlap). And also weakly similar to BAC18219|Q8FPT1 Putative transposase from Corynebacterium efficiens (446 aa), fasta scores: opt: 435, E(): 8.5e-17, (39.487% identity in 195 aa overlap);and O07182|Rv0797 Putative transposase of IS1547 from M. tuberculosis (364 aa), fasta scores: opt: 225, E(): 3.5e-05, (31.818% identity in 154 aa overlap). Highly similar to MUP043 but shorter. Contains a Pfam match to entry PF01548 Transposase_9, Transposase.; putative transposase 74138..74851 Mycobacterium ulcerans Agy99 2846559 YP_025558.1 CDS MUP036c NC_005916.1 74905 76239 R MUP036c, -, len: 444 aa. Probable transposase for IS2606, highly similar to Q9ZG97 Probable transposase from Mycobacterium ulcerans (444 aa), fasta scores: opt: 2878, E(): 5.8e-191, (98.198% identity in 444 aa overlap); and to O05912 Hypothetical 48.7 kDa protein (IS1554, transposase) from M. tuberculosis (439 aa), fasta scores: opt: 2042, E(): 2.3e-134, (71.628% identity in 435 aa overlap).; transposase for the insertion element IS2606 complement(74905..76239) Mycobacterium ulcerans Agy99 2846560 YP_025559.1 CDS MUP037 NC_005916.1 76556 77911 D MUP037, -, len: 451. Putative transposase, weakly similar to several transposases from Thermoanaerobacter tengcongensis e.g. Q8R7J7 Transposase (565 aa), fasta scores: opt: 296, E(): 1.8e-10, (22.826% identity in 368 aa overlap); and Q8R6J9 Transposase (565 aa), fasta scores: opt: 292, E(): 3.5e-10, (22.283% identity in 368 aa overlap). And also weakly similar to O70063 Putative transposase from Mycobacterium smegmatis (503 aa), fasta scores: opt: 203, E(): 9.1e-05, (26.790% identity in 377 aa overlap). And highly similar to MUP025 and MUP028 but shorter.; putative transposase 76556..77911 Mycobacterium ulcerans Agy99 2846569 YP_025560.1 CDS MUP038c NC_005916.1 78019 78924 R MUP038c, -, len: 301 aa. Possible thioesterase, similar to AAO65353|Q84CJ0 LanU-like protein from Streptomyces murayamaensis (249 aa), fasta scores: opt: 558, E(): 6.4e-30, (37.945% identity in 253 aa overlap); and weakly similar to weakly similar to Q98NH0 Lactone-specific esterase from Rhizobium loti (Mesorhizobium loti) (334 aa), fasta scores: opt: 175, E(): 0.00037, (23.675% identity in 283 aa overlap); Q9CD03|ML2603 Hypothetical protein from M. leprae (279 aa), fasta scores: opt: 153, E(): 0.0099, (33.051% identity in 118 aa overlap); Q9XAK3|SCO3599 Hypothetical protein from Streptomyces coelicolor (318 aa), fasta scores: opt: 160, E(): 0.0037, (28.261% identity in 276 aa overlap); and Q8PQF3 Hydrolase from Xanthomonas axonopodis (280 aa), fasta scores: opt: 158, E(): 0.0045, (30.769% identity in 130 aa overlap). Contains potential match to Pfam PF02230 Phospholipase/Carboxylesterase, Pfam PF00561 alpha/beta hydrolase fold.; thioesterase complement(78019..78924) Mycobacterium ulcerans Agy99 2846570 YP_025561.1 CDS mlsA2 NC_005916.1 79080 86312 R MUP039c, mlsA2, len: 2410 aa. Type I modular polyketide synthase, composed of one extender module (module 9); required for the synthesis of the mycolactone core in M. ulcerans (see Stinear et al., 2003). Similar to part of several e.g. Q9S0R8 Type I polyketide synthase AVES 1 from Streptomyces avermitilis (3972 aa), fasta scores: opt: 3342, E(): 2.1e-187, (40.556% identity in 2160 aa overlap); Q93NW8 AmphA from Streptomyces nodosus (1412 aa), fasta scores: opt: 3171, E(): 9.9e-178, (47.163% identity in 1304 aa overlap). Contains a Pfam match to entry PF00109 ketoacyl-synt: Beta-ketoacyl synthase, N-terminal domain and a Pfam match to entry PF02801 ketoacyl-synt_C: Beta-ketoacyl synthase, C-terminal domain. Contains a Pfam match entry PF00550 pp-binding: Phosphopantetheine attachment site and a Pfam match to entry PF00698 Acyl_transf: Acyl transferase domain.; Type I modular polyketide synthase complement(79080..86312) Mycobacterium ulcerans Agy99 2846549 YP_025562.1 CDS mlsA1 NC_005916.1 86299 137271 R MUP040c, mlsA1, len: 16990 aa. Type I modular polyketide synthase, composed of a loading module and eight extender modules; required for the synthesis of the mycolactone core in M. ulcerans (see Stinear et al., 2003). Similar to the modules in several other bacterial polyketide synthases e.g. O33954 Tylactone synthase starter module and modules 1 & 2 from Streptomyces fradiae (4472 aa), fasta scores: opt: 5121, E(): 0, (41.958% identity in 4066 aa overlap); Q9ALM3 Polyketide synthase extender modules 5-7 from Saccharopolyspora spinosa (4928 aa), fasta scores: opt: 4860, E(): 0, (41.813% identity in 5515 aa overlap); and Q9ADL6 Soraphen polyketide synthase A from Polyangium cellulosum (6315 aa), fasta scores: opt: 4448, E(): 0, (39.533% identity in 6420 aa overlap). Contains 9 x Pfam matches to entry PF00109 ketoacyl-synt: Beta-ketoacyl synthase, N-terminal domain and 9 x Pfam match to entry PF02801 ketoacyl-synt_C: Beta-ketoacyl synthase, C-terminal domain. Contains 9 x Pfam match entry PF00550 pp-binding: Phosphopantetheine attachment site and 9 x Pfam match to entry PF00698 Acyl_transf: Acyl transferase domain.; Type I modular polyketide synthase complement(86299..137271) Mycobacterium ulcerans Agy99 2846548 YP_025563.1 CDS MUP041c NC_005916.1 137361 137735 R MUP041c, -, len: 124 aa. Putative transposase, similar in part to several transposases e.g. BAC67824 Putative transposase from Streptomyces avermitilis (239 aa), fasta scores: opt: 263, E(): 3.7e-12, (50.617% identity in 81 aa overlap); Q8KRD5 ISRh1 transposase-like protein from Rhodococcus erythropolis (236 aa), fasta score: opt: 257, E(): 9e-12, (64.286% identity in 56 aa overlap); Q982I3 Transposase from plasmid pMLa of Rhizobium loti (Mesorhizobium loti) (185 aa), fasta scores: opt: 200, E(): 1.5e-07, (37.209% identity in 86 aa overlap). Highly similar to MUP033.; putative transposase complement(137361..137735) Mycobacterium ulcerans Agy99 2846571 YP_025564.1 CDS MUP042c NC_005916.1 137833 138372 R MUP042c, -, len: 179 aa. Putative transposase, weakly similar to several transposases from Xanthomonas e.g. Q8NL03 ISxac2 transposase from Xanthomonas axonopodis (275 aa), opt: 212, E(): 3.8e-07, (33.562% identity in 146 aa overlap); Q8PCU3 ISxcC1 transposase from Xanthomonas campestris (255 aa), fasta scores: opt: 198, E(): 3.4e-06, (34.286% identity in 105 aa overlap). Also weakly similar to Q9X9H9 IS1400 transposase B from Yersinia enterocolitica (294 aa), fasta scores: opt: 202, E(): 2e-06, (38.095% identity in 105 aa overlap). Almost identical to MUP034. Contains a Pfam match to entry PF00665 rve Integrase core domain.; putative transposase complement(137833..138372) Mycobacterium ulcerans Agy99 2846572 YP_025565.1 CDS MUP043 NC_005916.1 138963 140018 D MUP043, -, len: 351 aa. Putative transposase, similar to several putative transposases from Streptomyces e.g BAC68344|Q82Q86 Putative transposase from Streptomyces avermitilis (354 aa), fasta scores: opt: 1146, E(): 1.1e-63, (52.493% identity in 341 aa overlap); O69923 Putative transposase from Streptomyces coelicolor (379 aa), fasta scores: opt: 742, E(): 1.5e-38, (39.339% identity in 333 aa overlap). And similar to transposases from Corynebacterium efficiens e.g. BAC18308|Q8FTR3 Putative transposase (409 aa), fasta scores: opt: 993, E(): 4e-54, (45.210% identity in 334 aa overlap); BAC17059|Q8CMH0 Putative transposase (412 aa), fasta scores: opt: 947, E(): 2.9e-51, (43.413% identity in 334 aa overlap). Also weakly similar to Q8VJ30 IS1547, transposase from M. tuberculosis (596 aa), fasta scores: opt: 474, E(): 7.4e-22, (35.347% identity in 350 aa overlap). Contains a Pfam match to entry PF02371, Transposase_20, Transposase IS116/IS110/IS902 family. Highly similar to MUP035 but longer.; putative transposase 138963..140018 Mycobacterium ulcerans Agy99 2846573 YP_025566.1 CDS MUP044c NC_005916.1 140008 140148 R MUP044c, -, len: 46 aa. Putative truncated transposase, similar to several transposases from Xanthomonas e.g. Q8P9Q3 IS1477 transposase from Xanthomonas campestris (88 aa), fasta scores: opt: 106, E(): 0.2, (55.882% identity in 34 aa overlap); and Q8PRI1 ISxac2 transposase from Xanthomonas axonopodis (88 aa), fasta scores: opt: 104, E(): 0.29, (48.571% identity in 35 aa overlap). And some similarity to MUP051.; transposase complement(140008..140148) Mycobacterium ulcerans Agy99 2846596 YP_025567.1 CDS MUP045 NC_005916.1 140606 141592 D MUP045, -, len: 328 aa. Probable beta-ketoacyl synthase-like protein, weakly similar to Q9KHD3 Type III beta-ketoacyl synthase-like protein from Streptomyces griseus subsp. griseus (345 aa), fasta scores: opt: 238, E(): 1e-07, (26.282% identity in 312 aa overlap). Most similar to Q54816 Daunorubicin-doxorubicin polyketide synthase from Streptomyces peucetius (353 aa), fasta scores: opt: 301, E(): 9.1e-12, (31.672% identity in 341 aa overlap); and also similar to several 3-oxoacyl-(acyl-carrier-protein) synthases e.g. Q9A8I4 3-oxoacyl-(acyl-carrier-protein) synthase III from Caulobacter crescentus (322 aa), fasta scores: opt: 259, E(): 4.3e-09, (26.935% identity in 323 aa overlap).; beta-ketoacyl synthase-like protein 140606..141592 Mycobacterium ulcerans Agy99 2846597 YP_025568.1 CDS MUP046 NC_005916.1 142322 142615 D MUP046, -, len: 97 aa. Possible membrane protein, some similarity to Q92RR3 Hypothetical protein R00794 from Rhizobium meliloti (275 aa), E(): 26, (36.634% identity in 101 aa overlap). Contains a membrane spanning region between aa 24 to 46.; hypothetical protein 142322..142615 Mycobacterium ulcerans Agy99 2846598 YP_025569.1 CDS MUP047 NC_005916.1 143012 143716 D MUP047, -, len: 234 aa. Probable IS2404 transposase fragment, highly similar to O07439 Putative transposase from Mycobacterium ulcerans (327 aa), fasta scores: opt: 1339, E(): 5.2e-84, (91.880% identity in 234 aa overlap). Note that this homology extends across the stop codon and directly into MUP048, suggesting a possible readthrough of the TAG stop codon. No sequence errors have been identified and this insertion sequence containing the internal stop codon is repeated at two other locations on the plasmid, MUP029 + MUP030 and MUP062 + MUP063.; transposase for the insertion element IS2404 (fragment) 143012..143716 Mycobacterium ulcerans Agy99 2846599 YP_025570.1 CDS MUP048 NC_005916.1 143717 144058 D MUP048, -, len: 113 aa. Probable IS2404 transposase fragment, highly similar to the C-terminus of O07439 Putative transposase from M. ulcerans (327 aa), fasta scores: opt: 496, E(): 6.8e-31, (94.80% identity in 77 aa overlap). Note that this homology extends across the upstream TAG stop codon into the upstream MUP047, suggesting a possible readthrough of the previous stop codon. No sequence errors have been identified and this insertion sequence containing the internal stop codon is repeated at two other locations on the plasmid, MUP029 + MUP030 and MUP062 + MUP063.; transposase for the insertion element IS2404 (fragment) 143717..144058 Mycobacterium ulcerans Agy99 2846600 YP_025571.1 CDS MUP049c NC_005916.1 144304 144693 R MUP049c, -, len: 129 aa. Putative transposase, similar to several e.g. Q54335 Similar to ORF1 of the IS3 family from Streptomyces lividans (103 aa), fasta scores: opt: 225, E(): 2.9e-07, (44.565% identity in 92 aa overlap); and Q8XFW6 transposase from Brucella melitensis (93 aa), fasta scores: opt: 207, E(): 3.7e-06, (38.043% identity in 92 aa overlap); Q98A50 Transposase from Rhizobium loti (Mesorhizobium loti) (98 aa), fasta scores: opt: 204, E(): 6e-06, (37.234% identity in 94 aa overlap); Q8UJV4 Transposase from Agrobacterium tumefaciens plasmid AT (strain C58 / ATCC 33970) (96 aa), fasta scores: opt: 199, E(): 1.2e-05, (37.634% identity in 93 aa overlap). Contains a Pfam match to entry PF01527 Transposase_8, Transposase. Contains a helix turn helix motif between aa 58->79, tandard_deviations: 5.30, Score 1795.000.; putative transposase complement(144304..144693) Mycobacterium ulcerans Agy99 2846543 YP_025572.1 CDS MUP050 NC_005916.1 144660 145994 D MUP050, -, len: 444 aa. Probable transposase for IS2606, highly similar to Q9ZG97 Probable transposase from Mycobacterium ulcerans (444 aa), fasta scores: opt: 2878, E(): 2.7e-186, (98.423% identity in 444 aa overlap); and to O05912 Hypothetical 48.7 kDa protein (IS1554, transposase) from M. tuberculosis (439 aa), fasta scores: opt: 2053, E(): 1.2e-130, (70.805% identity in 435 aa overlap).; transposase for the insertion element IS2606 144660..145994 Mycobacterium ulcerans Agy99 2846544 YP_025573.1 CDS MUP051 NC_005916.1 146252 146533 D MUP051, -, len: 93 aa. Putative transposase, similar to several transposases from Rhizobium loti (Mesorhizobium loti) e.g. Q8KHM1 Putative insertion element transposase protein (88 aa), fasta scores: opt: 254, E(): 2.3e-10, (48.889% identity in 90 aa overlap). And weakly similar to several transposases from Yersinia e.g. O68709 Putative transposase from Yersinia pestis (88 aa), fasta scores: opt: 234, E(): 5.3e-09, (37.778% identity in 90 aa overlap). And some similarity to MUP044. Contains a Pfam match to entry PF01527, Transposase_8, Transposase. Contains helix turn helix motif between aa 27->48, Standard_deviations: 4.91, score 1680.00.; putative transposase 146252..146533 Mycobacterium ulcerans Agy99 2846545 YP_025574.1 CDS MUP052 NC_005916.1 146563 147396 D MUP052, -, len: 277 aa. Putative transposase, weakly similar to several transposases e.g Q89TF0 Blr2054 protein from Bradyrhizobium japonicum (290 aa), fasta scores: E(): 1.9e-27, (38.224% identity in 259 aa overlap); Q8U751 IS3 family transposase from Agrobacterium tumefaciens (strain C58 / ATCC 33970) (309 aa), fasta scores: opt: 549, E(): 1.3e-29, (39.068% identity in 279 aa overlap); Q8KJC6 Putative transposase for insertion sequence from Rhizobium loti (Mesorhizobium loti) (309 aa), fasta scores: opt: 528, E(): 2.5e-25 (35.836% identity in 293 aa overlap). Contains a Pfam match to PF00665 rve, Integrase core domain and a PS50316 histidine-rich region.; putative transposase 146563..147396 Mycobacterium ulcerans Agy99 2846546 YP_025575.1 CDS cyp150 NC_005916.1 147546 148859 R MUP053c, cyp150, len: 437 aa. Probable cyp150, cytochrome p450 (EC 1.14.-.-), similar to O08464|C140_MYCTU|Rv1880c Probable cytochrome P450 140 from M. tuberculosis (438 aa), fasta scores: opt: 1873, E(): 6.7e-117, (62.529% identity in 435 aa overlap). And weakly similar to numerous others e.g. Q89G11|BAC51802 Bll6537 protein from Bradyrhizobium japonicum (433 aa), fasta scores: opt: 755, E(): 6.1e-41 (34.925% identity in 398 aa overlap); and Q9X8Q3 Putative cytochrome P450 from Streptomyces coelicolor (411 aa), fasta scores: E(): 2.9e-36, (34.063% identity in 411 aa overlap). Contains a Pfam match to entry PF00067, Cytochrome P450. Belongs to the cytochrome P450 family.; cytochrome p450 150 cyp150 complement(147546..148859) Mycobacterium ulcerans Agy99 2846568 YP_025576.1 CDS MUP054c NC_005916.1 148856 149359 R MUP054c, -, len: 167 aa. Possible integrase fragment, weakly similar to several e.g. AAO22922 Integrase/recombinase from Myxococcus xanthus (300 aa), fasta scores: opt: 176, E(): 0.00018, (37.4% identity in 107 aa overlap); Q8NNZ9 Integrase from Corynebacterium glutamicum (Brevibacterium flavum) (308 aa), fasta scores: opt: 169, E(): 0.00054, (33.33% identity in 84 aa overlap); and Q8L3A3 Putative integrase/recombinase Xer from Bacillus stearothermophilus (304 aa), fasta scores: opt: 168, E(): 0.00063, (33.02% identity in 106 aa overlap). Contains a Pfam match to entry PF00589 Phage_integrase Phage integrase family.; integrase fragment complement(148856..149359) Mycobacterium ulcerans Agy99 2846547 YP_025577.1 CDS MUP055 NC_005916.1 149323 150657 D MUP055, -, len: 444 aa. Probable transposase for IS2606, highly similar to Q9ZG97 Probable transposase from Mycobacterium ulcerans (444 aa), fasta scores: opt: 2879, E(): 1.7e-184, (98.198% identity in 444 aa overlap); and to O05912 Hypothetical 48.7 kDa protein (IS1554, transposase) from M. tuberculosis (439 aa), fasta scores: opt: 2055, E(): 1.9e-129, (70.575% identity in 435 aa overlap).; transposase for the insertion element IS2606 149323..150657 Mycobacterium ulcerans Agy99 2846577 YP_025578.1 CDS MUP056c NC_005916.1 150862 151242 R MUP056c, -, len: 126 aa. Hypothetical protein.; hypothetical protein complement(150862..151242) Mycobacterium ulcerans Agy99 2846578 YP_025579.1 CDS MUP057c NC_005916.1 151341 152117 R MUP057c, -, len: 258 aa. Possible lipoprotein, weakly similar to Q49597 MK35 lipoprotein precursor from Mycobacterium kansasii (225 aa), fasta scores: opt: 139, E(): 0.21, (27.059% identity in 170 aa overlap); and weakly similar at the N-terminus to Q8XCD1 Hypothetical protein z2967 from Escherichia coli O157:H7 (115 aa), fasta scores: opt: 148, E(): 0.037, (29.167% identity in 96 aa overlap). Has signal peptide and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.; lipoprotein complement(151341..152117) Mycobacterium ulcerans Agy99 2846579 YP_025580.1 CDS MUP058c NC_005916.1 152314 153351 R MUP058c, -, len: 345 aa. Possible site-specific recombinase, weakly similar to several e.g. Q8U9U6 Site-specific recombinase from Agrobacterium tumefaciens (strain C58 / ATCC 33970) (331 aa), fasta scores: opt: 278, E(): 1.6e-11, (26.441% identity in 295 aa overlap)Q9JW14 Putative integrase/recombinase from Neisseria meningitidis (serogroup A) (305 aa), fasta scores: opt: 271, E(): 4.6e-11, (28.808% identity in 302 aa overlap); and O31206 Site-specific recombinase from Proteus mirabilis (313 aa), fasta scores: opt: 258, E(): 3.7e-10 (31.010% identity in 287 aa overlap). Contains Pfam match entries to PF02899 Phage integrase and PF00589 Phage integrase family.; site-specific recombinase complement(152314..153351) Mycobacterium ulcerans Agy99 2846580 YP_025581.1 CDS MUP059c NC_005916.1 153595 154641 R MUP059c, -, len: 348 aa. Probable transposase for IS2404, highly similar to O07439 Putative transposase from Mycobacterium ulcerans (327 aa), fasta scores: opt: 1839, E(): 2.4e-119, (92.949% identity in 312 aa overlap).; transposase for the insertion element IS2404 complement(153595..154641) Mycobacterium ulcerans Agy99 2846586 YP_025582.1 CDS MUP060 NC_005916.1 155147 155668 D MUP060, -, len: 173 aa. Probable IS2606 transposase fragment, highly similar to the N-terminus of Q9ZG97 Probable transposase from Mycobacterium ulcerans (444 aa), fasta scores: opt: 886, E(): 1.8e-52, (84.615% identity in 169 aa overlap); and to the N-terminus of O05912 Hypothetical 48.7 kDa protein (IS1554, transposase) from M. tuberculosis (439 aa), fasta scores: opt: 506, E(): 1.1e-26, (60.000% identity in 135 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses MUP060 and MUP061, no sequence errors have been identified to account for this.; transposase for the insertion element IS2606 (fragment) 155147..155668 Mycobacterium ulcerans Agy99 2846587 YP_025583.1 CDS MUP061 NC_005916.1 155574 156482 D MUP061, -, len: 302 aa. Probable IS2606 transposase fragment, highly similar to the C-terminus of Q9ZG97 Probable transposase from Mycobacterium ulcerans (444 aa), fasta scores: opt: 1990, E(): 2.8e-132, (98.675% identity in 302 aa overlap); and to the C-terminus of O05912 Hypothetical 48.7 kDa protein (IS1554, transposase) from M. tuberculosis (439 aa), fasta scores: opt: 1544, E(): 6.9e-101, (75.166% identity in 302 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses MUP060 and MUP061, no sequence errors have been identified to account for this.; transposase for the insertion element IS2606 (fragment) 155574..156482 Mycobacterium ulcerans Agy99 2846588 YP_025584.1 CDS MUP062 NC_005916.1 156842 157546 D MUP062, -, len: 234 aa. Probable IS2404 transposase fragment, highly similar to O07439 Putative transposase from M. ulcerans (327 aa), fasta scores: opt: 1333, E(): 2.7e-84, (91.880% identity in 234 aa overlap). Note that this homology extends across the stop codon and directly into MUP063, suggesting a possible readthrough of the TAG stop codon. No sequence errors have been identified and this insertion sequence containing the internal stop codon is repeated at two other locations on the plasmid, MUP029 + MUP030 and MUP047 + MUP048.; transposase for the insertion element IS2404 (fragment) 156842..157546 Mycobacterium ulcerans Agy99 2846589 YP_025585.1 CDS MUP063 NC_005916.1 157547 157888 D MUP063, -, len: 113 aa. Probable IS2404 transposase fragment, highly similar to the C-terminus of O07439 Putative transposase from M. ulcerans (327 aa), fasta scores: opt: 503, E(): 1.9e-31, (96.104% identity in 77 aa overlap). Note that this homology extends across the upstream TAG stop codon into the upstream MUP062, suggesting a possible readthrough of the previous stop codon. No sequence errors have been identified and this insertion sequence containing the internal stop codon is repeated at two other locations on the plasmid, MUP029 + MUP030 and MUP047 + MUP048.; transposase for the insertion element IS2404 (fragment) 157547..157888 Mycobacterium ulcerans Agy99 2846590 YP_025586.1 CDS MUP064c NC_005916.1 157889 158251 R MUP064c, -, len: 120 aa. Possible conserved membrane protein, shows some similarity with other putative membrane proteins e.g. C-terminus of O06345|Rv3482c Probable conserved membrane protein from M. tuberculosis (260 aa), fasta scores: opt: 129, E(): 0.39, (30.233% identity in 86 aa overlap). And show some similarity to Mycobacteriophage proteins e.g. AAN12600 Gp39 from Mycobacteriophage (132 aa), fasta score: E(): 0.055, (40.000% identity in 50 aa overlap). Contains several membrane spanning regions towards the C-terminus.; hypothetical protein complement(157889..158251) Mycobacterium ulcerans Agy99 2846601 YP_025587.1 CDS MUP065c NC_005916.1 158471 159352 R MUP065c, -, len: 293 aa. Conserved hypothetical protein, weakly similar at the N-terminus to several hypothetical proteins e.g. Q8U687 Hypothetical protein Atu6048 from Agrobacterium tumefaciens (strain C58 / ATCC 33970) (836 aa), fasta scores: opt: 223, E(): 8.2e-07, (30.000% identity in 170 aa overlap); and also some similarity at the N-terminus to several helicases e.g. AAO50170 Putative helicase from Streptomyces violaceoruber (866 aa), fasta scores: E(): 1.1e-09, (33.032% identity in 221 aa overlap); and P44701|SRMB_HAEIN ATP-dependent RNA helicase srmB homolog from Haemophilus influenzae (439 aa), fasta scores: opt: 124, E(): 1.9, (31.429% identity in 105 aa overlap). Contains a Pfam match to entry PF00270, DEAD/DEAD box helicase.; hypothetical protein complement(158471..159352) Mycobacterium ulcerans Agy99 2846602 YP_025588.1 CDS MUP066c NC_005916.1 159824 160330 R MUP066c, -, len: 168 aa. Conserved hypothetical protein, weakly similar to several bacteriophage-related proteins e.g. P03796|Y77_BPT7 Hypothetical gene 7.7 protein from Bacteriophage T7 (130 aa), fasta scores: opt: 171, E(): 0.0002, (32.075% identity in 106 aa overlap); and Q8XUF2 Putative bacteriophage-related protein from Ralstonia solanacearum (Pseudomonas solanacearum) (183 aa), fasta scores: opt: 163, E(): 0.00089, (32.773% identity in 119 aa overlap). Contains a Prosite match to entry PS00029 LEUCINE_ZIPPER Leucine zipper pattern.; hypothetical protein complement(159824..160330) Mycobacterium ulcerans Agy99 2846603 YP_025589.1 CDS MUP067c NC_005916.1 160417 161049 R MUP067c, -, len: 210 aa. Conserved hypothetical protein, weak similarity to N-terminus of spermidine synthases e.g. Q82EU4 Putative spermidine synthase from Streptomyces avermitilis (548 aa), FASTA scores: opt: 124, E(): 2.5, (34.091% identity in 88 aa overlap); and weak similarity to N-terminus of O87639|SCO3941 Putative serine/threonine phosphatase from Streptomyces coelicolor (368 aa), fasta scores: opt: 112, E(): 12, (27.919% identity in 197 aa overlap).; hypothetical protein complement(160417..161049) Mycobacterium ulcerans Agy99 2846604 YP_025590.1 CDS MUP068c NC_005916.1 161085 162215 R MUP068c, -, len: 376 aa. Conserved membrane protein, similar to several Mycobacterial membrane proteins e.g. O53933|Rv1782 (506 aa) from M. tuberculosis, fasta scores: opt: 763, E(): 1.8e-34, (41.935% identity in 341 aa overlap); Q9Z5I3 Hypothetical 53.9 kDa protein (Conserved membrane protein) from M. leprae (506 aa), fasta scores: opt: 733, E(): 4.2e-32, (38.596% identity in 399 aa overlap); and the M. tuberculosis proteins Rv3895c, Rv0283, Rv3869 and Rv3450c. And also weakly similar to other bacterial hypothetical proteins e.g. Q8NSU6 Hypothetical protein Cgl0571 from Corynebacterium glutamicum (419 aa), fasta scores: opt: 349, E(): 4.3e-12, (31.818% identity in 242 aa overlap). Contains a Pfam match to entry PF05108 DUF690, protein of unknown function and a Prosite match to entry PS50310 alanine-rich region. Contains a possible N-terminal signal sequence.; hypothetical protein complement(161085..162215) Mycobacterium ulcerans Agy99 2846605 YP_025591.1 CDS MUP069c NC_005916.1 162445 163779 R MUP069c, -, len: 444 aa. Probable transposase for IS2606, highly similar to Q9ZG97 Probable transposase from Mycobacterium ulcerans (444 aa), fasta scores: opt: 2867, E(): 8e-184, (97.973% identity in 444 aa overlap); and to O05912 Hypothetical 48.7 kDa protein (IS1554, transposase) from M. tuberculosis (439 aa), fasta scores: opt: 2043, E(): 9.7e-129, (70.345% identity in 435 aa overlap).; transposase for the insertion element IS2606 complement(162445..163779) Mycobacterium ulcerans Agy99 2846581 YP_025592.1 CDS MUP070c NC_005916.1 163727 164824 R MUP070c, -, len: 365 aa. Conserved hypothetical protein, weakly similar to part of Q9KYC4 Hypothetical protein SCO6906 from Streptomyces coelicolor (529 aa), fasta scores: opt: 185, E(): 0.0015, (27.305% identity in 282 aa overlap). Similarity to Q9KYC4 begins in the upstream CDS, and this could be accounted for by a frameshift, although the sequence has been checked and no discrepancy was found.; hypothetical protein complement(163727..164824) Mycobacterium ulcerans Agy99 2846582 YP_025593.1 CDS MUP071c NC_005916.1 164673 165089 R MUP071c, -, len: 138 aa. Conserved hypothetical protein, weakly similar to the N-terminus of Q9KYC4 Hypothetical protein SCO6906 from Streptomyces coelicolor (529 aa), fasta scores: opt: 153, E(): 0.0041, (29.060% identity in 117 aa overlap). Similarity to Q9KYC4 continues in the following CDS, and this could be accounted for by a frameshift, although the sequence has been checked and no discrepancy was found.; hypothetical protein complement(164673..165089) Mycobacterium ulcerans Agy99 2846583 YP_025594.1 CDS MUP072c NC_005916.1 165161 166357 R MUP072c, -, len: 398 aa. Conserved hypothetical protein, some similarity to proteins from Caenorhabditis elegans e.g. Q20739 F54B11.2 protein (304 aa), fasta scores: opt: 192, E(): 0.017, (39.216% identity in 102 aa overlap); and CAE11316 C. elegans COL-185 protein (305 aa), fasta scores: opt: 185, E(): 0.039, (38.235% identity in 102 aa overlap). Contains a Prosite match to entry PS50310 ALA_RICH Alanine-rich region.; hypothetical protein complement(165161..166357) Mycobacterium ulcerans Agy99 2846584 YP_025595.1 CDS MUP073c NC_005916.1 166354 167547 R MUP073c, -, len: 397 aa. Conserved hypothetical protein. Some similarity to part of Q9Z5I9|IF2_MYCLE|ML1556 Translation initiation factor IF-2 from M. leprae (924 aa), fasta scores: opt: 228, E(): 0.0023, (26.997% identity in 363 aa overlap). Contains a Prosite match to entry PS00015 NUCLEAR Bipartite nuclear targeting sequence.; hypothetical protein complement(166354..167547) Mycobacterium ulcerans Agy99 2846585 YP_025596.1 CDS MUP074c NC_005916.1 167568 168152 R MUP074c, -, len: 194 aa. Possible membrane protein, weakly similar to several bacterial membrane and hypothetical proteins e.g. O53200|Rv2473 Hypothetical protein from M. tuberculosis (238 aa), fasta scores: opt: 155, E(): 0.18, (25.248% identity in 202 aa overlap); O86726 Hypothetical protein SCO6544 from Streptomyces coelicolor (226 aa), fasta scores: opt: 155, E(): 0.18, (25.405% identity in 185 aa overlap). Contains a Prosite match to entry PS50310 ALA_RICH Alanine-rich region. Contains hydrophobic region in the N-terminus.; hypothetical protein complement(167568..168152) Mycobacterium ulcerans Agy99 2846591 YP_025597.1 CDS MUP075c NC_005916.1 168149 168487 R MUP075c, -, len: 112 aa. Hypothetical protein, some similarity to part of Q8Y3B8 Probable substrate-binding transmembrane protein from Ralstonia solanacearum (Pseudomonas solanacearum) (801 aa), fasta scores: opt: 131, E(): 1.1, (33.33% identity in 102 aa overlap). Contains a Prosite match to entry PS50310 ALA_RICH Alanine-rich region.; hypothetical protein complement(168149..168487) Mycobacterium ulcerans Agy99 2846592 YP_025598.1 CDS MUP076c NC_005916.1 168487 169158 R MUP076c, -, len: 223 aa. Possible membrane protein, contains a membrane spanning region between aa 57 to 79. Contains a Prosite match to entry PS50099 PRO_RICH Proline-rich region.; hypothetical protein complement(168487..169158) Mycobacterium ulcerans Agy99 2846593 YP_025599.1 CDS MUP077c NC_005916.1 169192 169584 R MUP077c, -, len: 130 aa. Conserved hypothetical protein, weakly similar to P71600|Y030_MYCTU|Rv0030 Hypothetical protein from M. tuberculosis (109 aa), fasta scores: opt: 122, E(): 0.42, (27.660% identity in 94 aa overlap); and O69744|Rv3880c Hypothetical protein from M. tuberculosis (115 aa), fasta scores: opt: 112, E(): 2.1, (29.000% identity in 100 aa overlap).; hypothetical protein complement(169192..169584) Mycobacterium ulcerans Agy99 2846594 YP_025600.1 CDS MUP078c NC_005916.1 169759 171342 R MUP078c, -, len: 527 aa. Conserved hypothetical protein, weakly similar to Q10691|YK83_MYCTU|Rv2083 Hypothetical protein from M. tuberculosis (314 aa), fasta scores: opt: 229, E(): 0.0041, (32.597% identity in 181 aa overlap); Q8WTL8 Hypothetical 37.7 kDa protein from Caenorhabditis elegans (435 aa), fasta scores: opt: 233, E(): 0.0034, (32.093% identity in 215 aa overlap); and also weakly similar to several PGRS-family proteins e.g. O53884|Rv0872c|PE_PGRS15 PGRS-family protein from M. tuberculosis (606 aa), fasta scores: opt: 220, E(): 0.016, (25.686% identity in 510 aa overlap). Contains a Prosite match to entry PS00029 LEUCINE_ZIPPER Leucine zipper pattern.; hypothetical protein complement(169759..171342) Mycobacterium ulcerans Agy99 2846595 YP_025601.1 CDS MUP079c NC_005916.1 171361 171660 R MUP079c, -, len: 99 aa. Conserved hypothetical protein, weakly similar to P71598|Y028_MYCTU|Rv0028 Hypothetical protein from M. tuberculosis (101 aa), fasta scores: opt: 93, E(): 11, (32.558% identity in 86 aa overlap); and some similarity to Q51652 DNA primase (Fragment) from Escherichia coli (489 aa), fasta scores: opt: 101, E(): 11, (29.762% identity in 84 aa overlap).; hypothetical protein complement(171361..171660) Mycobacterium ulcerans Agy99 2846565 YP_025602.1 CDS MUP080c NC_005916.1 171667 171939 R MUP080c, -, len: 90 aa. Conserved hypothetical protein, weakly similar to P71597|Y027_MYCTU|Rv0027 Hypothetical protein from M. tuberculosis (105 aa), fasta scores: opt: 117, E(): 0.3, (26.966% identity in 89 aa overlap).; hypothetical protein complement(171667..171939) Mycobacterium ulcerans Agy99 2846566 YP_025603.1 CDS MUP081c NC_005916.1 172002 173546 R MUP081c, -, len: 514 aa. Conserved hypothetical protein, weakly similar to P71596|YO26_MYCTU|Rv0026 Hypothetical protein from M. tuberculosis (448 aa), fasta scores: opt: 501 E(): 6.9e-15, (31.989% identity in 372 aa overlap). Contains a Prosite match to entry PS00017 ATP_GTP_A- ATP/GTP-binding site motif A (P-loop).; hypothetical protein complement(172002..173546) Mycobacterium ulcerans Agy99 2846567 YP_904252.1 CDS dnaA NC_008611.1 1 1533 D binds to the dnaA-box as an ATP-bound complex at the origin of replication during the initiation of chromosomal replication; can also affect transcription of multiple genes including itself.; chromosomal replication initiation protein 1..1533 Mycobacterium ulcerans Agy99 4553245 YP_904253.1 CDS dnaN NC_008611.1 2072 3280 D binds the polymerase to DNA and acts as a sliding clamp; DNA polymerase III subunit beta 2072..3280 Mycobacterium ulcerans Agy99 4553246 YP_904254.1 CDS recF NC_008611.1 3301 4458 D Required for DNA replication; binds preferentially to single-stranded, linear DNA; recombination protein F 3301..4458 Mycobacterium ulcerans Agy99 4553247 YP_904255.1 CDS MUL_0004 NC_008611.1 4455 5018 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein 4455..5018 Mycobacterium ulcerans Agy99 4553248 YP_904256.1 CDS gyrB NC_008611.1 5228 7306 D negatively supercoils closed circular double-stranded DNA; DNA gyrase subunit B 5228..7306 Mycobacterium ulcerans Agy99 4553249 YP_904257.1 CDS gyrA NC_008611.1 7346 9865 D negatively supercoils closed circular double-stranded DNA; DNA gyrase subunit A 7346..9865 Mycobacterium ulcerans Agy99 4553250 YP_904258.1 CDS MUL_0007 NC_008611.1 9949 10947 D membrane protein; hypothetical protein 9949..10947 Mycobacterium ulcerans Agy99 4553251 YP_904259.1 CDS MUL_0010 NC_008611.1 11361 12197 D cytoplasmic protein; hypothetical protein 11361..12197 Mycobacterium ulcerans Agy99 4553254 YP_904260.1 CDS MUL_0011 NC_008611.1 12225 12986 R cytoplasmic protein; transcription; transcriptional regulator complement(12225..12986) Mycobacterium ulcerans Agy99 4553255 YP_904261.1 CDS MUL_0012 NC_008611.1 13097 13501 R cytoplasmic protein; hypothetical protein complement(13097..13501) Mycobacterium ulcerans Agy99 4553256 YP_904262.1 CDS ppiA NC_008611.1 13691 14239 D Also detected in the extracellular matrix by proteomics.; cytoplasmic protein; Ppiases accelerate the folding of proteins [catalytic activity: cis-trans isomerization of proline imidic peptide bonds in oligopeptides]; peptidyl-prolyl cis-trans isomerase A, PpiA 13691..14239 Mycobacterium ulcerans Agy99 4553257 YP_904263.1 CDS MUL_0014 NC_008611.1 14250 14675 R membrane protein; hypothetical protein complement(14250..14675) Mycobacterium ulcerans Agy99 4553258 YP_904264.1 CDS MUL_0015 NC_008611.1 14831 15112 R integral membrane protein involved in inhibition of the Z-ring formation; putative septation inhibitor protein complement(14831..15112) Mycobacterium ulcerans Agy99 4552742 YP_904265.1 CDS MUL_0016 NC_008611.1 15241 15984 D membrane protein; hypothetical protein 15241..15984 Mycobacterium ulcerans Agy99 4552743 YP_904266.1 CDS trpG NC_008611.1 16022 16708 D aminodeoxychorismate synthase subunit PabA; with PabB catalyzes the formation of 4-amino-4-deoxychorismate from chorismate and glutamine in para-aminobenzoate synthesis; PabA provides the glutamine amidotransferase activity; para-aminobenzoate synthase component II 16022..16708 Mycobacterium ulcerans Agy99 4552744 YP_904267.1 CDS pknB NC_008611.1 16686 18566 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in signal transduction (via phosphorylation) thought to regulate cell division/differentiation. can phosphorylate the peptide substrate myelin basic protein (MBP) [catalytic activity: ATP + a protein = ADP + a phosphoprotein]; serine/threonine-protein kinase B PknB complement(16686..18566) Mycobacterium ulcerans Agy99 4552745 YP_904268.1 CDS pknA NC_008611.1 18563 19921 R membrane protein; involved in signal transduction (via phosphorylation) thought to regulate morphological changes associated with cell division/differentiation process. phosphorylates at serine and threonine residues [catalytic activity: ATP + a protein = ADP + a phosphoprotein]; serine/threonine-protein kinase a PknA complement(18563..19921) Mycobacterium ulcerans Agy99 4552746 YP_904269.1 CDS pbpA NC_008611.1 19918 21393 R membrane protein; involved in peptidoglycan synthesis (at the final stages) cell wall formation; PbpA is supposed to be responsible for the determination of the rod shape of the cell. its synthesizes cross-linked peptidoglycan from lipid intermediates.; penicillin-binding protein PbpA complement(19918..21393) Mycobacterium ulcerans Agy99 4552747 YP_904270.1 CDS rodA NC_008611.1 21390 22799 R membrane protein; this is a septum-peptidoglycan biosynthetic protein, involved in cell wall formation. plays a role in the stabilization of the FtsZ ring during cell division.; cell division protein RodA complement(21390..22799) Mycobacterium ulcerans Agy99 4552748 YP_904271.1 CDS pstP NC_008611.1 22796 24355 R Detected in the membrane fraction by proteomics (LC- MS/MS) Also detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; involved in regulation (using dephosphorylation of a specific phosphorylated substrate); serine/threonine phosphatase PstP complement(22796..24355) Mycobacterium ulcerans Agy99 4552749 YP_904272.1 CDS MUL_0023 NC_008611.1 24352 24819 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(24352..24819) Mycobacterium ulcerans Agy99 4552750 YP_904273.1 CDS MUL_0024 NC_008611.1 24953 26551 R Detected in the cytoplasmic and secreted fractions by 2D-LC-MS/MS.; secreted protein; function unknown, contains FHA domain; hypothetical protein complement(24953..26551) Mycobacterium ulcerans Agy99 4552751 YP_904274.1 CDS MUL_0025 NC_008611.1 26845 28179 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 26845..28179 Mycobacterium ulcerans Agy99 4552752 YP_904275.1 CDS MUL_0027 NC_008611.1 28617 29372 D cytoplasmic protein; glycosylase 28617..29372 Mycobacterium ulcerans Agy99 4552754 YP_904276.1 CDS MUL_0028 NC_008611.1 29488 30162 R cytoplasmic protein; short-chain dehydrogenase complement(29488..30162) Mycobacterium ulcerans Agy99 4552755 YP_904277.1 CDS MUL_0029 NC_008611.1 30440 31192 D cytoplasmic protein; hypothetical protein 30440..31192 Mycobacterium ulcerans Agy99 4552756 YP_904278.1 CDS MUL_0032 NC_008611.1 32216 32920 R membrane protein; hypothetical protein complement(32216..32920) Mycobacterium ulcerans Agy99 4552164 YP_904279.1 CDS proV_1 NC_008611.1 33021 34220 R membrane protein; thought to be involved in active transport of osmoprotectant (glycine betaine/carnitine/choline/L- proline) across the membrane (import) responsible for energy coupling to the transport system.; osmoprotectant (glycine betaine/carnitine/choline/L-proline) transport ATP-binding protein ABC transporter ProV complement(33021..34220) Mycobacterium ulcerans Agy99 4552165 YP_904280.1 CDS MUL_0034 NC_008611.1 34213 34860 R membrane protein; maybe involved in active transport of osmoprotectant (glycine betaine/carnitine/choline/L- proline) across the membrane (import) responsible for the translocation of the substrate across the membrane.; ABC transporter permease complement(34213..34860) Mycobacterium ulcerans Agy99 4552166 YP_904281.1 CDS MUL_0035 NC_008611.1 35179 35586 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; DNA-binding protein 35179..35586 Mycobacterium ulcerans Agy99 4552167 YP_904282.1 CDS MUL_0036 NC_008611.1 35688 36635 R hypothetical protein complement(35688..36635) Mycobacterium ulcerans Agy99 4552168 YP_904283.1 CDS amt_1 NC_008611.1 36765 38078 D membrane protein; thought to be involved in transport of ammonium across the membrane (export) responsible for the translocation of the substrate across the membrane.; ammonium-transport integral membrane protein, Amt 36765..38078 Mycobacterium ulcerans Agy99 4552169 YP_904284.1 CDS MUL_0038 NC_008611.1 38088 38387 R cytoplasmic protein; hypothetical protein complement(38088..38387) Mycobacterium ulcerans Agy99 4552170 YP_904285.1 CDS MUL_0039 NC_008611.1 38384 39301 R N-term truncated by frame-shift mutation; cytoplasmic protein; hypothetical protein complement(38384..39301) Mycobacterium ulcerans Agy99 4552171 YP_904286.1 CDS whiB5 NC_008611.1 39468 39884 R cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein (Whib-like), WhiB5 complement(39468..39884) Mycobacterium ulcerans Agy99 4552172 YP_904287.1 CDS MUL_0041 NC_008611.1 40027 40818 D cytoplasmic protein; maybe involved in transcriptional mechanism.; transcriptional regulatory protein 40027..40818 Mycobacterium ulcerans Agy99 4552173 YP_904288.1 CDS MUL_0042 NC_008611.1 40815 41642 D secreted protein; function unknown. the P60 protein is a major extracellular protein may be involved in the invasion of host cells.; secreted protein P60-related protein 40815..41642 Mycobacterium ulcerans Agy99 4552174 YP_904289.1 CDS MUL_0043 NC_008611.1 41639 42019 D cytoplasmic protein; hypothetical protein 41639..42019 Mycobacterium ulcerans Agy99 4552175 YP_904290.1 CDS MUL_0044 NC_008611.1 42094 43359 D cytoplasmic protein; hypothetical protein 42094..43359 Mycobacterium ulcerans Agy99 4552176 YP_904291.1 CDS MUL_0045 NC_008611.1 43402 43719 D cytoplasmic protein; hypothetical protein 43402..43719 Mycobacterium ulcerans Agy99 4552177 YP_904292.1 CDS MUL_0046 NC_008611.1 43716 44120 D cytoplasmic protein; hypothetical protein 43716..44120 Mycobacterium ulcerans Agy99 4552178 YP_904293.1 CDS MUL_0047 NC_008611.1 44248 45354 D hypothetical protein 44248..45354 Mycobacterium ulcerans Agy99 4552179 YP_904294.1 CDS MUL_0048 NC_008611.1 45442 45762 D cytoplasmic protein; hypothetical protein 45442..45762 Mycobacterium ulcerans Agy99 4552180 YP_904295.1 CDS MUL_0049 NC_008611.1 45887 46069 D secreted protein; hypothetical protein 45887..46069 Mycobacterium ulcerans Agy99 4552181 YP_904296.1 CDS MUL_0050 NC_008611.1 46161 46934 R cytoplasmic protein; hypothetical protein complement(46161..46934) Mycobacterium ulcerans Agy99 4552182 YP_904297.1 CDS MUL_0051 NC_008611.1 46937 48229 R membrane protein; integral membrane protein complement(46937..48229) Mycobacterium ulcerans Agy99 4552183 YP_904298.1 CDS MUL_0052 NC_008611.1 48389 48994 D cytoplasmic protein; hypothetical protein 48389..48994 Mycobacterium ulcerans Agy99 4550136 YP_904299.1 CDS MUL_0053 NC_008611.1 48981 49976 R membrane protein; hypothetical protein complement(48981..49976) Mycobacterium ulcerans Agy99 4550137 YP_904300.1 CDS MUL_0054 NC_008611.1 50029 50379 R membrane protein; hypothetical protein complement(50029..50379) Mycobacterium ulcerans Agy99 4550138 YP_904301.1 CDS mtc28 NC_008611.1 50464 51597 R secreted protein; secreted proline rich protein Mtc28 complement(50464..51597) Mycobacterium ulcerans Agy99 4550139 YP_904302.1 CDS leuS NC_008611.1 51796 54726 D leucine--tRNA ligase; LeuRS; class-I aminoacyl-tRNA synthetase; charges leucine by linking carboxyl group to alpha-phosphate of ATP and then transfers aminoacyl-adenylate to its tRNA; due to the large number of codons that tRNA(Leu) recognizes, the leucyl-tRNA synthetase does not recognize the anticodon loop of the tRNA, but instead recognition is dependent on a conserved discriminator base A37 and a long arm; an editing domain hydrolyzes misformed products; in Methanothermobacter thermautotrophicus this enzyme associates with prolyl-tRNA synthetase; leucyl-tRNA synthetase 51796..54726 Mycobacterium ulcerans Agy99 4550140 YP_904303.1 CDS MUL_0057 NC_008611.1 54756 55421 R membrane protein; function unknown, possibly involved in cellular metabolism.; short chain dehydrogenase complement(54756..55421) Mycobacterium ulcerans Agy99 4550141 YP_904304.1 CDS MUL_0058 NC_008611.1 55462 55965 R Detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; possibly involved in transcriptional mechanism.; transcriptional regulatory protein complement(55462..55965) Mycobacterium ulcerans Agy99 4550142 YP_904305.1 CDS MUL_0061 NC_008611.1 58626 59390 R cytoplasmic protein; involved in transcriptional mechanism.; GntR family transcriptional regulator complement(58626..59390) Mycobacterium ulcerans Agy99 4550145 YP_904306.1 CDS MUL_0062 NC_008611.1 59501 60298 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase complement(59501..60298) Mycobacterium ulcerans Agy99 4550146 YP_904307.1 CDS MUL_0063 NC_008611.1 60330 61241 R cytoplasmic protein; function unknown, probably involved in lipid biosynthesis.; hydrolase complement(60330..61241) Mycobacterium ulcerans Agy99 4550147 YP_904308.1 CDS ino1 NC_008611.1 61287 62405 R involved in phosphatidylinositol (pi) biosynthetic pathway [catalytic activity: d-glucose 6-phosphate = 1L- myo-inositol 1-phosphate]; myo-inositol-1-phosphate synthase Ino1 complement(61287..62405) Mycobacterium ulcerans Agy99 4550148 YP_904309.1 CDS MUL_0065 NC_008611.1 62467 63009 R cytoplasmic protein; hypothetical protein complement(62467..63009) Mycobacterium ulcerans Agy99 4550149 YP_904310.1 CDS MUL_0066 NC_008611.1 63165 64034 R membrane protein; hypothetical protein complement(63165..64034) Mycobacterium ulcerans Agy99 4550150 YP_904311.1 CDS MUL_0067 NC_008611.1 64147 64581 D cytoplasmic protein; hypothetical protein 64147..64581 Mycobacterium ulcerans Agy99 4550151 YP_904312.1 CDS ponA1 NC_008611.1 65069 67111 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in peptidoglycan synthesis (at the final stages), cell wall formation. synthesis of cross-linked peptidoglycan from the lipid intermediates. the enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits); bifunctional penicillin-binding protein 1A/1B PonA1 65069..67111 Mycobacterium ulcerans Agy99 4550152 YP_904313.1 CDS MUL_0069 NC_008611.1 67108 68748 D membrane protein; hypothetical protein 67108..68748 Mycobacterium ulcerans Agy99 4550153 YP_904314.1 CDS MUL_0070 NC_008611.1 68748 69386 D cytoplasmic protein; transcriptional regulatory protein 68748..69386 Mycobacterium ulcerans Agy99 4550154 YP_904315.1 CDS rpsF NC_008611.1 69510 69800 D binds cooperatively with S18 to the S15-16S complex, allowing platform assembly to continue with S11 and S21; 30S ribosomal protein S6 69510..69800 Mycobacterium ulcerans Agy99 4551749 YP_904316.1 CDS ssb NC_008611.1 69892 70407 D binds to single stranded DNA and may facilitate the binding and interaction of other proteins to DNA; single-stranded DNA-binding protein 69892..70407 Mycobacterium ulcerans Agy99 4551750 YP_904317.1 CDS rpsR NC_008611.1 70453 70707 D binds as a heterodimer with protein S6 to the central domain of the 16S rRNA; helps stabilize the platform of the 30S subunit; 30S ribosomal protein S18 70453..70707 Mycobacterium ulcerans Agy99 4551751 YP_904318.1 CDS rplI NC_008611.1 70754 71212 D in Escherichia coli this protein is wrapped around the base of the L1 stalk; 50S ribosomal protein L9 70754..71212 Mycobacterium ulcerans Agy99 4551752 YP_904319.1 CDS dnaB NC_008611.1 71743 73125 D cytoplasmic protein; participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity. the intein is an endonuclease (potential); replicative DNA helicase DnaB 71743..73125 Mycobacterium ulcerans Agy99 4551753 YP_904320.1 CDS MUL_0076 NC_008611.1 73690 74349 D cytoplasmic protein; hypothetical protein 73690..74349 Mycobacterium ulcerans Agy99 4551754 YP_904321.1 CDS MUL_0077 NC_008611.1 74478 75104 R cytoplasmic protein; function unknown, possibly involved in cellular metabolism.; hydrolase complement(74478..75104) Mycobacterium ulcerans Agy99 4551755 YP_904322.1 CDS MUL_0080 NC_008611.1 77264 77878 R cytoplasmic protein; transcriptional regulator complement(77264..77878) Mycobacterium ulcerans Agy99 4551757 YP_904323.1 CDS MUL_0081 NC_008611.1 77913 78218 D cytoplasmic protein; hypothetical protein 77913..78218 Mycobacterium ulcerans Agy99 4551758 YP_904324.1 CDS MUL_0084 NC_008611.1 80415 80999 D cytoplasmic protein; hypothetical protein 80415..80999 Mycobacterium ulcerans Agy99 4551761 YP_904325.1 CDS MUL_0086 NC_008611.1 82839 83144 D cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulator 82839..83144 Mycobacterium ulcerans Agy99 4551763 YP_904326.1 CDS MUL_0087 NC_008611.1 83141 83557 D cytoplasmic protein; hypothetical protein 83141..83557 Mycobacterium ulcerans Agy99 4551764 YP_904327.1 CDS MUL_0089 NC_008611.1 85879 87126 D cytoplasmic protein; hypothetical protein 85879..87126 Mycobacterium ulcerans Agy99 4549582 YP_904328.1 CDS rfbD NC_008611.1 87171 88127 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; may form an ATP-driven O-antigen/lipopolysaccharide export apparatus, in association with RfbE. responsible for the translocation of the substrate across the membrane.; O-antigen/lipopolysaccharide transport integral membrane protein ABC transporter RfbD complement(87171..88127) Mycobacterium ulcerans Agy99 4549583 YP_904329.1 CDS MUL_0091 NC_008611.1 87998 88972 R membrane protein; possibly involved in rhamnose biosynthesis; L-rhamnosyltransferase complement(87998..88972) Mycobacterium ulcerans Agy99 4549584 YP_904330.1 CDS rfbE NC_008611.1 88920 89762 R membrane protein; may form an ATP-driven O-antigen/lipopolysaccharide export apparatus, in association with RfbD. responsible for energy coupling to the transport system.; O-antigen/lipopolysaccharide transport ATP- binding protein ABC transporter RfbE complement(88920..89762) Mycobacterium ulcerans Agy99 4549585 YP_904331.1 CDS MUL_0093 NC_008611.1 89766 90299 R cytoplasmic protein; hypothetical protein complement(89766..90299) Mycobacterium ulcerans Agy99 4549586 YP_904332.1 CDS MUL_0094 NC_008611.1 90312 92285 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; transmembrane protein complement(90312..92285) Mycobacterium ulcerans Agy99 4549587 YP_904333.1 CDS MUL_0095 NC_008611.1 92416 93612 D membrane protein; function unknown, probably involved in cellular metabolism.; aminotransferase 92416..93612 Mycobacterium ulcerans Agy99 4549588 YP_904334.1 CDS MUL_0096 NC_008611.1 93652 94644 R membrane protein; function unknown, probably involved in cellular metabolism.; oxidoreductase complement(93652..94644) Mycobacterium ulcerans Agy99 4549589 YP_904335.1 CDS MUL_0098 NC_008611.1 95273 96187 D PPE62; membrane protein; PPE family protein 95273..96187 Mycobacterium ulcerans Agy99 4549591 YP_904336.1 CDS MUL_0099 NC_008611.1 96405 97451 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 96405..97451 Mycobacterium ulcerans Agy99 4549592 YP_904337.1 CDS MUL_0101 NC_008611.1 98998 100044 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 98998..100044 Mycobacterium ulcerans Agy99 4551431 YP_904338.1 CDS fba NC_008611.1 101494 102528 R catalyzes the formation of glycerone phosphate and glyceraldehyde 3-phosphate from fructose 1,6, bisphosphate; fructose-bisphosphate aldolase complement(101494..102528) Mycobacterium ulcerans Agy99 4551433 YP_904339.1 CDS MUL_0104 NC_008611.1 102629 103321 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; transmembrane protein 102629..103321 Mycobacterium ulcerans Agy99 4551434 YP_904340.1 CDS MUL_0105 NC_008611.1 103310 104449 R cytoplasmic protein; glycosyl hydrolase complement(103310..104449) Mycobacterium ulcerans Agy99 4551435 YP_904341.1 CDS eutC NC_008611.1 109181 109954 D catalyzes the formation of acetaldehyde from ethanolamine; ethanolamine ammonia-lyase small subunit 109181..109954 Mycobacterium ulcerans Agy99 4551439 YP_904342.1 CDS MUL_0110 NC_008611.1 109957 111162 R membrane protein; von Willebrand factor type A domain-containing protein complement(109957..111162) Mycobacterium ulcerans Agy99 4551440 YP_904343.1 CDS MUL_0111 NC_008611.1 111169 111843 R Also detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; function unknown, probably involved in cellular metabolism.; membrane oxidoreductase complement(111169..111843) Mycobacterium ulcerans Agy99 4551441 YP_904344.1 CDS MUL_0112 NC_008611.1 111857 112735 R membrane protein; function unknown, probably involved in cellular metabolism.; membrane oxidoreductase complement(111857..112735) Mycobacterium ulcerans Agy99 4551442 YP_904345.1 CDS MUL_0113 NC_008611.1 112736 113326 R cytoplasmic protein; hypothetical protein complement(112736..113326) Mycobacterium ulcerans Agy99 4551443 YP_904346.1 CDS MUL_0114 NC_008611.1 113433 114212 R membrane protein; hypothetical protein complement(113433..114212) Mycobacterium ulcerans Agy99 4551444 YP_904347.1 CDS coxL NC_008611.1 114216 116606 R cytoplasmic protein; function unknown, probably involved in cellular metabolism [catalytic activity: CO + H(2)O + acceptor = CO(2) + reduced acceptor]; carbon monoxyde dehydrogenase large chain CoxL complement(114216..116606) Mycobacterium ulcerans Agy99 4551445 YP_904348.1 CDS coxS NC_008611.1 116603 117082 R cytoplasmic protein; probably involved in cellular metabolism [catalytic activity: CO + H(2)O + acceptor = CO(2) + reduced acceptor]; carbon monoxyde dehydrogenase small chain CoxS complement(116603..117082) Mycobacterium ulcerans Agy99 4551446 YP_904349.1 CDS MUL_0119 NC_008611.1 119275 120207 D cytoplasmic protein; possibly involved in transcriptional mechanism.; LysR family transcriptional regulator 119275..120207 Mycobacterium ulcerans Agy99 4551449 YP_904350.1 CDS secE2 NC_008611.1 120225 120440 D secreted protein; thought to be involved in protein transport (export); protein transport protein SecE2 120225..120440 Mycobacterium ulcerans Agy99 4552843 YP_904351.1 CDS MUL_0121 NC_008611.1 120549 121205 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; possibly causes methylation of RNA.; RNA methyltransferase complement(120549..121205) Mycobacterium ulcerans Agy99 4552844 YP_904352.1 CDS MUL_0122 NC_008611.1 121190 122083 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(121190..122083) Mycobacterium ulcerans Agy99 4552845 YP_904353.1 CDS pyrE NC_008611.1 122095 122634 R involved in fifth step of pyrimidine biosynthesis; converts orotidine 5'-phosphate and diphosphate to orotate and 5-phospho-alpha-D-ribose 1-diphosphate; orotate phosphoribosyltransferase complement(122095..122634) Mycobacterium ulcerans Agy99 4552846 YP_904354.1 CDS MUL_0124 NC_008611.1 122621 123394 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown, but possibly involvement in lipid metabolism.; ketoacyl reductase complement(122621..123394) Mycobacterium ulcerans Agy99 4552847 YP_904355.1 CDS MUL_0125 NC_008611.1 123439 124293 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(123439..124293) Mycobacterium ulcerans Agy99 4552848 YP_904356.1 CDS echA8_1 NC_008611.1 124651 125481 D cytoplasmic protein; oxidizes fatty acids using specific components (by similarity) [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]; enoyl-CoA hydratase, EchA8 124651..125481 Mycobacterium ulcerans Agy99 4552849 YP_904357.1 CDS clpB NC_008611.1 125478 128024 R Detected in the cytoplasmic fraction by LCMSMS. Also detected in the extracellular matrix and the membrane fraction by proteomics.; membrane protein; ATPase subunit of an intracellular ATP-dependent protease. in M. tuberculosis H37Rv it is regulated positively by SigH and negatively by HspR.; endopeptidase ATP binding protein (chain B) ClpB complement(125478..128024) Mycobacterium ulcerans Agy99 4552850 YP_904358.1 CDS MUL_0128 NC_008611.1 128351 129532 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; monooxygenase 128351..129532 Mycobacterium ulcerans Agy99 4552851 YP_904359.1 CDS MUL_0129 NC_008611.1 129607 130497 D cytoplasmic protein; possible carbohydrate transport and metabolism; hypothetical protein 129607..130497 Mycobacterium ulcerans Agy99 4552852 YP_904360.1 CDS MUL_0130 NC_008611.1 130824 131870 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 130824..131870 Mycobacterium ulcerans Agy99 4552853 YP_904361.1 CDS MUL_0131 NC_008611.1 132228 133562 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 132228..133562 Mycobacterium ulcerans Agy99 4552854 YP_904362.1 CDS MUL_0132 NC_008611.1 133754 134947 R cytoplasmic protein; required for the transposition of insertion element IS2404. ORF is extended by 50 aa.; transposase for IS2404 complement(133754..134947) Mycobacterium ulcerans Agy99 4552855 YP_904363.1 CDS MUL_0133 NC_008611.1 135369 135608 D cytoplasmic protein; hypothetical protein 135369..135608 Mycobacterium ulcerans Agy99 4552856 YP_904364.1 CDS MUL_0135 NC_008611.1 138020 139048 D cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator 138020..139048 Mycobacterium ulcerans Agy99 4552858 YP_904365.1 CDS MUL_0136 NC_008611.1 139396 140241 D membrane protein; function unknown, possibly involved in cellular metabolism.; short-chain type dehydrogenase/reductase 139396..140241 Mycobacterium ulcerans Agy99 4552859 YP_904366.1 CDS MUL_0137 NC_008611.1 140258 140908 D membrane protein; function unknown, contains NADH-flavin reductase domain; hypothetical protein 140258..140908 Mycobacterium ulcerans Agy99 4552860 YP_904367.1 CDS metE NC_008611.1 141241 143616 D catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine to form methionine; 5- methyltetrahydropteroyltriglutamate/homocysteine S-methyltransferase 141241..143616 Mycobacterium ulcerans Agy99 4552861 YP_904368.1 CDS MUL_0139 NC_008611.1 143694 145415 R membrane protein; hypothetical protein complement(143694..145415) Mycobacterium ulcerans Agy99 4552862 YP_904369.1 CDS MUL_0140 NC_008611.1 145487 145996 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(145487..145996) Mycobacterium ulcerans Agy99 4552863 YP_904370.1 CDS ppdK NC_008611.1 146094 147734 D catalyzes the formation of phosphoenolpyruvate from pyruvate; pyruvate phosphate dikinase 146094..147734 Mycobacterium ulcerans Agy99 4552864 YP_904371.1 CDS MUL_0142 NC_008611.1 147731 148336 D cytoplasmic protein; hypothetical protein 147731..148336 Mycobacterium ulcerans Agy99 4552865 YP_904372.1 CDS ephC NC_008611.1 149589 150515 R cytoplasmic protein; thought to be involved in detoxification reactions following oxidative damage to lipids [catalytic activity: an epoxide + H(2)O = a glycol]; epoxide hydrolase EphC complement(149589..150515) Mycobacterium ulcerans Agy99 4552867 YP_904373.1 CDS MUL_0145 NC_008611.1 151048 151551 D cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator 151048..151551 Mycobacterium ulcerans Agy99 4552868 YP_904374.1 CDS MUL_0146 NC_008611.1 151637 152113 R cytoplasmic protein; hypothetical protein complement(151637..152113) Mycobacterium ulcerans Agy99 4552869 YP_904375.1 CDS bpoB NC_008611.1 152240 153115 D cytoplasmic protein; supposed involved in detoxification reactions.; peroxidase BpoB 152240..153115 Mycobacterium ulcerans Agy99 4552870 YP_904376.2 CDS gnd2 NC_008611.1 153112 154134 R similar to full-length Gnd, these proteins seems to have a truncated C-terminal 6PGD domainin; in Methylobacillus flagellatus this gene is essential for NAD+-dependent oxidation of 6-phosphogluconate; 6-phosphogluconate dehydrogenase complement(153112..154134) Mycobacterium ulcerans Agy99 4552871 YP_904377.1 CDS zwf1 NC_008611.1 154149 155165 R catalyzes the formation of D-glucono-1,5-lactone 6-phosphate from D-glucose 6-phosphate; glucose-6-phosphate 1-dehydrogenase complement(154149..155165) Mycobacterium ulcerans Agy99 4552872 YP_904378.1 CDS MUL_0150 NC_008611.1 155832 156539 D cytoplasmic protein; hypothetical protein 155832..156539 Mycobacterium ulcerans Agy99 4552873 YP_904379.1 CDS MUL_0151 NC_008611.1 156572 156751 D cytoplasmic protein; hypothetical protein 156572..156751 Mycobacterium ulcerans Agy99 4552874 YP_904380.1 CDS MUL_5079 NC_008611.1 156733 157434 D cytoplasmic protein; hypothetical protein 156733..157434 Mycobacterium ulcerans Agy99 4552875 YP_904381.1 CDS MUL_0152 NC_008611.1 157531 157860 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; hypothetical protein complement(157531..157860) Mycobacterium ulcerans Agy99 4552876 YP_904382.1 CDS MUL_0153 NC_008611.1 157936 158232 R cytoplasmic protein; hypothetical protein complement(157936..158232) Mycobacterium ulcerans Agy99 4552877 YP_904383.1 CDS MUL_0155 NC_008611.1 158704 160224 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; dioxygenase 158704..160224 Mycobacterium ulcerans Agy99 4552676 YP_904384.1 CDS MUL_0156 NC_008611.1 160169 160714 D cytoplasmic protein; hypothetical protein 160169..160714 Mycobacterium ulcerans Agy99 4552677 YP_904385.1 CDS MUL_0157 NC_008611.1 160720 161154 R membrane protein; hypothetical protein complement(160720..161154) Mycobacterium ulcerans Agy99 4552678 YP_904386.1 CDS MUL_0158 NC_008611.1 161225 161998 R hypothetical protein complement(161225..161998) Mycobacterium ulcerans Agy99 4552679 YP_904387.1 CDS MUL_0160 NC_008611.1 163531 163977 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(163531..163977) Mycobacterium ulcerans Agy99 4552681 YP_904388.1 CDS MUL_0161 NC_008611.1 163982 164170 R cytoplasmic protein; hypothetical protein complement(163982..164170) Mycobacterium ulcerans Agy99 4552682 YP_904389.1 CDS MUL_0163 NC_008611.1 165556 166890 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 165556..166890 Mycobacterium ulcerans Agy99 4552684 YP_904390.1 CDS MUL_0165 NC_008611.1 167198 168244 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(167198..168244) Mycobacterium ulcerans Agy99 4552686 YP_904391.1 CDS MUL_0166 NC_008611.1 168649 169749 R translation-associated GTPase; the crystal structure of the Haemophilus influenzae YchF protein showed similarity to the yeast structure (PDB: 1NI3); fluorescence spectroscopy revealed nucleic acid binding; the yeast protein YBR025c interacts with the translation elongation factor eEF1; GTP-dependent nucleic acid-binding protein EngD complement(168649..169749) Mycobacterium ulcerans Agy99 4552687 YP_904392.1 CDS MUL_0167 NC_008611.1 169882 171102 D membrane protein; hypothetical protein 169882..171102 Mycobacterium ulcerans Agy99 4552688 YP_904393.1 CDS ispH NC_008611.1 171116 172114 R catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP); functions in the nonmevalonate isoprenoid biosynthesis pathway; 4-hydroxy-3-methylbut-2-enyl diphosphate reductase complement(171116..172114) Mycobacterium ulcerans Agy99 4552689 YP_904394.1 CDS MUL_0169 NC_008611.1 172224 172841 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; hypothetical protein 172224..172841 Mycobacterium ulcerans Agy99 4552690 YP_904395.1 CDS xseA NC_008611.1 172838 174085 D bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides; exodeoxyribonuclease VII large subunit 172838..174085 Mycobacterium ulcerans Agy99 4552691 YP_904396.1 CDS xseB NC_008611.1 174069 174323 D catalyzes the bidirectional exonucleolytic cleavage of DNA; exodeoxyribonuclease VII small subunit 174069..174323 Mycobacterium ulcerans Agy99 4553964 YP_904397.1 CDS MUL_0172 NC_008611.1 174378 175472 D cytoplasmic protein; function unknown, probably involved in cellular metabolism; cholesterol dehydrogenase 174378..175472 Mycobacterium ulcerans Agy99 4553965 YP_904398.1 CDS MUL_0173 NC_008611.1 175647 175910 D cytoplasmic protein; hypothetical protein 175647..175910 Mycobacterium ulcerans Agy99 4553966 YP_904399.1 CDS MUL_0174 NC_008611.1 176005 176271 R cytoplasmic protein; hypothetical protein complement(176005..176271) Mycobacterium ulcerans Agy99 4553967 YP_904400.1 CDS MUL_0175 NC_008611.1 176368 177414 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(176368..177414) Mycobacterium ulcerans Agy99 4553968 YP_904401.1 CDS MUL_0177 NC_008611.1 179542 180699 D membrane protein; hypothetical protein 179542..180699 Mycobacterium ulcerans Agy99 4553970 YP_904402.1 CDS MUL_0178 NC_008611.1 180677 181477 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(180677..181477) Mycobacterium ulcerans Agy99 4553971 YP_904403.1 CDS MUL_0179 NC_008611.1 181505 182215 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(181505..182215) Mycobacterium ulcerans Agy99 4553972 YP_904404.1 CDS glpX NC_008611.1 182221 183309 D type II fructose 1,6-bisphosphatae; in Escherichia coli this protein forms a dimer and binds manganese; fructose 1,6-bisphosphatase II 182221..183309 Mycobacterium ulcerans Agy99 4553973 YP_904405.1 CDS fumC NC_008611.1 183347 184777 D class II family (does not require metal); tetrameric enzyme; fumarase C; reversibly converts (S)-malate to fumarate and water; functions in the TCA cycle; fumarate hydratase 183347..184777 Mycobacterium ulcerans Agy99 4553974 YP_904406.1 CDS MUL_0182 NC_008611.1 184874 185854 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; membrane glycine and proline rich protein 184874..185854 Mycobacterium ulcerans Agy99 4553975 YP_904407.1 CDS MUL_0184 NC_008611.1 187386 188261 R membrane protein; probably involved in carbohydrate degradation. may hydrolyse the glycosidic bond between two or more carbohydrates or between a carbohydrate and a non- carbohydrate moiety.; glycosyl hydrolase complement(187386..188261) Mycobacterium ulcerans Agy99 4553977 YP_904408.1 CDS MUL_0186 NC_008611.1 189626 189829 R hypothetical protein complement(189626..189829) Mycobacterium ulcerans Agy99 4553979 YP_904409.1 CDS desA2 NC_008611.1 189944 190771 R Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); cytoplasmic protein; thought to catalyze the principal conversion of saturated fatty acids to unsaturated fatty acids. thought to convert stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons delta-9 and delta-10 of the acyl chain [catalytic activity: stearoyl-[acyl-carrier protein] + AH2 + O2 = oleoyl-[acyl-carrier protein] + a + 2 H2O]; acyl-[acyl-carrier protein] desaturase DesA2 complement(189944..190771) Mycobacterium ulcerans Agy99 4553980 YP_904410.1 CDS glyA NC_008611.1 190828 192108 R catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate; serine hydroxymethyltransferase complement(190828..192108) Mycobacterium ulcerans Agy99 4553981 YP_904411.1 CDS coaA NC_008611.1 192270 193208 D catalyzes the formation of (R)-4'-phosphopantothenate in coenzyme A biosynthesis; pantothenate kinase 192270..193208 Mycobacterium ulcerans Agy99 4551412 YP_904412.1 CDS MUL_0190 NC_008611.1 193472 194452 R PE_PGRS20; membrane protein; PE-PGRS family protein complement(193472..194452) Mycobacterium ulcerans Agy99 4551413 YP_904413.1 CDS MUL_0191 NC_008611.1 194658 195176 R membrane protein; hypothetical protein complement(194658..195176) Mycobacterium ulcerans Agy99 4551414 YP_904414.1 CDS MUL_0192 NC_008611.1 195261 196628 R membrane protein; hypothetical protein complement(195261..196628) Mycobacterium ulcerans Agy99 4551415 YP_904415.1 CDS uppS NC_008611.1 196867 197682 R cytoplasmic protein; catalyzes the first committed step in the synthesis of decaprenyl diphosphate, a molecule which has a central role in the biosynthesis of most features of the mycobacterial cell wall. adds one isoprene unit to omega,E- geranyl diphosphate. in mycobacterium; short (C15) chain Z-isoprenyl diphosphate synthase, UppS complement(196867..197682) Mycobacterium ulcerans Agy99 4551416 YP_904416.1 CDS MUL_0194 NC_008611.1 197802 198542 D potential role involved in virulence; membrane protein; hemolysin-like protein 197802..198542 Mycobacterium ulcerans Agy99 4551417 YP_904417.1 CDS MUL_0195 NC_008611.1 198567 198950 R cytoplasmic protein; hypothetical protein complement(198567..198950) Mycobacterium ulcerans Agy99 4551418 YP_904418.1 CDS MUL_0196 NC_008611.1 198964 200988 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; transmembrane protein complement(198964..200988) Mycobacterium ulcerans Agy99 4551419 YP_904419.1 CDS MUL_0197 NC_008611.1 200975 201274 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(200975..201274) Mycobacterium ulcerans Agy99 4551420 YP_904420.1 CDS mca NC_008611.1 201271 202143 R cytoplasmic protein; mycothiol-dependent detoxification enzyme, involved in mycothiol biosynthesis.; mycothiol conjugate amidase Mca complement(201271..202143) Mycobacterium ulcerans Agy99 4551421 YP_904421.1 CDS MUL_0199 NC_008611.1 202285 202719 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 202285..202719 Mycobacterium ulcerans Agy99 4551422 YP_904422.1 CDS greA NC_008611.1 202951 203445 D necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites; arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus; transcription elongation factor GreA 202951..203445 Mycobacterium ulcerans Agy99 4551423 YP_904423.1 CDS metB NC_008611.1 203528 204694 R catalyzes the formation of cystathionine from L-cysteine and O-succinyl-L-homoserine; cystathionine gamma-synthase complement(203528..204694) Mycobacterium ulcerans Agy99 4551424 YP_904424.1 CDS pra NC_008611.1 204794 205552 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; proline-rich antigen Pra-like protein complement(204794..205552) Mycobacterium ulcerans Agy99 4551425 YP_904425.1 CDS cbs NC_008611.1 205748 207142 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (LC-MS/MS); cytoplasmic protein; thought to be involved in homocysteine transulfuration [catalytic activity: L-serine + L- homocysteine = cystathionine + H2O]; cystathionine beta-synthase complement(205748..207142) Mycobacterium ulcerans Agy99 4551426 YP_904426.1 CDS lipU NC_008611.1 207201 208079 R Detected in the cytoplasmic fraction by proteomics.; cytoplasmic protein; hydrolyses lipids; lipase LipU complement(207201..208079) Mycobacterium ulcerans Agy99 4551427 YP_904427.1 CDS MUL_0206 NC_008611.1 208533 209477 D secreted protein; hypothetical protein 208533..209477 Mycobacterium ulcerans Agy99 4551428 YP_904428.1 CDS fadA3 NC_008611.1 209587 210804 D Catalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation; acetyl-CoA acetyltransferase 209587..210804 Mycobacterium ulcerans Agy99 4551429 YP_904429.1 CDS MUL_0208 NC_008611.1 210985 211827 R membrane protein; hypothetical protein complement(210985..211827) Mycobacterium ulcerans Agy99 4551430 YP_904430.1 CDS echA9 NC_008611.1 212075 213112 D catalyzes the formation of 3-hydroxy-2-methylpropanoate from 3-hydroxy-2-methylpropanoyl-CoA; 3-hydroxyisobutyryl-CoA hydrolase 212075..213112 Mycobacterium ulcerans Agy99 4553912 YP_904431.1 CDS echA8 NC_008611.1 213124 213897 D Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase 213124..213897 Mycobacterium ulcerans Agy99 4553913 YP_904432.1 CDS MUL_0211 NC_008611.1 213909 215705 D membrane protein; hypothetical protein 213909..215705 Mycobacterium ulcerans Agy99 4553914 YP_904433.1 CDS MUL_0212 NC_008611.1 215905 217815 D PE_PGRS9_3; membrane protein; PE-PGRS family protein 215905..217815 Mycobacterium ulcerans Agy99 4553915 YP_904434.1 CDS MUL_0214 NC_008611.1 219507 220553 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 219507..220553 Mycobacterium ulcerans Agy99 4553917 YP_904435.1 CDS MUL_0215 NC_008611.1 221430 221813 R cytoplasmic protein; hypothetical protein complement(221430..221813) Mycobacterium ulcerans Agy99 4553918 YP_904436.1 CDS MUL_0216 NC_008611.1 221813 222388 R cytoplasmic protein; hypothetical protein complement(221813..222388) Mycobacterium ulcerans Agy99 4553919 YP_904437.1 CDS lpqV NC_008611.1 222531 222998 D membrane protein; lipoprotein LpqV 222531..222998 Mycobacterium ulcerans Agy99 4553920 YP_904438.1 CDS MUL_0218 NC_008611.1 223101 224165 D cytoplasmic protein; hypothetical protein 223101..224165 Mycobacterium ulcerans Agy99 4553921 YP_904439.1 CDS MUL_0220 NC_008611.1 225057 225935 R cytoplasmic protein; hypothetical protein complement(225057..225935) Mycobacterium ulcerans Agy99 4553923 YP_904440.1 CDS MUL_0221 NC_008611.1 225968 226441 R membrane protein; hypothetical protein complement(225968..226441) Mycobacterium ulcerans Agy99 4553924 YP_904441.1 CDS MUL_0223 NC_008611.1 228677 229381 D cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 228677..229381 Mycobacterium ulcerans Agy99 4553926 YP_904442.1 CDS MUL_0224 NC_008611.1 229406 229723 D cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 229406..229723 Mycobacterium ulcerans Agy99 4549632 YP_904443.1 CDS MUL_0225 NC_008611.1 229777 230658 R Detected in the membrane fraction by 2D-LC-MS/MS.; cytoplasmic protein; hypothetical protein complement(229777..230658) Mycobacterium ulcerans Agy99 4549633 YP_904444.1 CDS MUL_0226 NC_008611.1 230651 231547 R cytoplasmic protein; acid phosphatase complement(230651..231547) Mycobacterium ulcerans Agy99 4549634 YP_904445.1 CDS aslA NC_008611.1 231548 233098 R cytoplasmic protein; thought to play an important role in the mineralization of sulfates [catalytic activity: a phenol sulfate + H2O = a phenol + sulfate]; arylsulfatase, AslA complement(231548..233098) Mycobacterium ulcerans Agy99 4549635 YP_904446.1 CDS fadD14 NC_008611.1 234378 236009 R activates fatty acids by binding to coenzyme A; long-chain-fatty-acid--CoA ligase complement(234378..236009) Mycobacterium ulcerans Agy99 4549637 YP_904447.1 CDS MUL_0230 NC_008611.1 236131 237330 R hypothetical protein complement(236131..237330) Mycobacterium ulcerans Agy99 4549638 YP_904448.1 CDS MUL_0232 NC_008611.1 237857 238903 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(237857..238903) Mycobacterium ulcerans Agy99 4549640 YP_904449.1 CDS ctpE NC_008611.1 239476 241851 R membrane protein; metal cation-transporting ATPase; possibly catalyzes the transport of undeterminated metal cation with hydrolyse of ATP [catalytic activity: ATP + H(2)O + undeterminated metal cation(in) = ADP + phosphate + undeterminated metal cation(out)]; metal cation transporter ATPase p-type CtpE complement(239476..241851) Mycobacterium ulcerans Agy99 4549641 YP_904450.1 CDS MUL_0235 NC_008611.1 243670 244788 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(243670..244788) Mycobacterium ulcerans Agy99 4549643 YP_904451.1 CDS echA6 NC_008611.1 244795 245526 R Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase complement(244795..245526) Mycobacterium ulcerans Agy99 4549644 YP_904452.1 CDS accD3 NC_008611.1 245553 247034 D membrane protein; this protein is a component of the acetyl coenzyme a carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA [catalytic activity ATP + acetyl-CoA + HCO(3)(- ) = ADP + phosphate + malonyl-CoA]; acetyl-coenzyme a carboxylase carboxyl transferase (subunit beta) AccD3 245553..247034 Mycobacterium ulcerans Agy99 4549645 YP_904453.1 CDS MUL_0240 NC_008611.1 248092 249591 D C-term truncated 20aa.; membrane protein; function unknown, but maybe involved in efflux system (probably sugar or drug transport); hypothetical protein 248092..249591 Mycobacterium ulcerans Agy99 4549646 YP_904454.1 CDS bioF2_1 NC_008611.1 249613 250875 D cytoplasmic protein; could be involved in biotin biosynthesis (at the first step) [catalytic activity: 6-carboxyhexanoyl-CoA + L- alanine = 8-amino-7-oxononanoate + CoA + CO2]; 8-amino-7-oxononanoate synthase 249613..250875 Mycobacterium ulcerans Agy99 4549647 YP_904455.1 CDS cysM NC_008611.1 251051 251995 D cytoplasmic protein; involved in cysteine biosynthesis [catalytic activity : O3-acetyl-L-serine + H(2)S = L-cysteine + acetate]; cysteine synthase B 251051..251995 Mycobacterium ulcerans Agy99 4549648 YP_904456.1 CDS MUL_0245 NC_008611.1 253251 253772 R cytoplasmic protein; involved in transcriptional mechanism; transcriptional regulatory protein complement(253251..253772) Mycobacterium ulcerans Agy99 4549937 YP_904457.1 CDS MUL_0247 NC_008611.1 254272 255651 D cytoplasmic protein; hypothetical protein 254272..255651 Mycobacterium ulcerans Agy99 4549938 YP_904458.1 CDS prrA NC_008611.1 255802 256512 D Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); membrane protein; transcriptional regulator part of the two component regulatory system PrrA/PrrB. thought to be involved in the environmental adaptation , specifically in an early phase of the intracellular growth.; two component response transcriptional regulatory protein PrrA 255802..256512 Mycobacterium ulcerans Agy99 4549939 YP_904459.1 CDS prrB NC_008611.1 256527 257867 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; sensor part of the two component regulatory system PrrA/PrrB. thought to be involved in the environmental adaptation , specifically in an early phase of the intracellular growth.; two component sensor histidine kinase PrrB 256527..257867 Mycobacterium ulcerans Agy99 4549940 YP_904460.1 CDS MUL_0251 NC_008611.1 258526 258759 R membrane protein; hypothetical protein complement(258526..258759) Mycobacterium ulcerans Agy99 4549942 YP_904461.1 CDS ompA NC_008611.1 258772 259770 R membrane protein; the protein behaved as a porin of low specific activity. structural protein that may protect the integrity of the bacterium.; outer membrane protein OmpA complement(258772..259770) Mycobacterium ulcerans Agy99 4549943 YP_904462.1 CDS MUL_0253 NC_008611.1 259883 260146 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein 259883..260146 Mycobacterium ulcerans Agy99 4549944 YP_904463.1 CDS MUL_0254 NC_008611.1 260170 261744 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 260170..261744 Mycobacterium ulcerans Agy99 4549945 YP_904464.1 CDS gltA NC_008611.1 261749 263044 R type II enzyme; in Escherichia coli this enzyme forms a trimer of dimers which is allosterically inhibited by NADH and competitively inhibited by alpha-ketoglutarate; allosteric inhibition is lost when Cys206 is chemically modified which also affects hexamer formation; forms oxaloacetate and acetyl-CoA and water from citrate and coenzyme A; functions in TCA cycle, glyoxylate cycle and respiration; enzyme from Helicobacter pylori is not inhibited by NADH; type II citrate synthase complement(261749..263044) Mycobacterium ulcerans Agy99 4549946 YP_904465.1 CDS MUL_0256 NC_008611.1 263432 264901 R Also detected in the cytoplamic fraction by LCMSMS; membrane protein; function unknown, probably involved in cellular metabolism.; monooxygenase complement(263432..264901) Mycobacterium ulcerans Agy99 4549947 YP_904466.1 CDS pdxH NC_008611.1 265165 265653 R cytoplasmic protein; involved in biosynthesis of pyridoxine (vitamin B6) and pyridoxal phosphate. oxidize Pnp and PMP into pyridoxal 5'-phosphate (PLP)[catalytic activity: pyridoxamine 5'-phosphate + H(2)O + O(2) = pyridoxal 5'- phosphate + NH(3) + H(2)O(2)]; pyridoxamine 5'-phosphate oxidase PdxH complement(265165..265653) Mycobacterium ulcerans Agy99 4549948 YP_904467.1 CDS citA NC_008611.1 266751 267605 D forms citrate from oxaloacetate and acetyl-CoA; functions in TCA cycle; citrate synthase 2 266751..267605 Mycobacterium ulcerans Agy99 4549950 YP_904468.1 CDS MUL_0261 NC_008611.1 268076 269122 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(268076..269122) Mycobacterium ulcerans Agy99 4549952 YP_904469.1 CDS MUL_0263 NC_008611.1 273137 273595 D Detected in the cytoplasmic and secreted fractions by 2D-LC-MS/MS.; cytoplasmic protein; hypothetical protein 273137..273595 Mycobacterium ulcerans Agy99 4551600 YP_904470.1 CDS fprB NC_008611.1 273672 275357 R cytoplasmic protein; serves as the first electron transfer protein in all the P450 systems [catalytic activity: reduced adrenodoxin + NADP+ = oxidized adrenodoxin + NADPH]; NADPH:adrenodoxin oxidoreductase FprB complement(273672..275357) Mycobacterium ulcerans Agy99 4551601 YP_904471.1 CDS MUL_0265 NC_008611.1 275376 276401 R cytoplasmic protein; hypothetical protein complement(275376..276401) Mycobacterium ulcerans Agy99 4551602 YP_904472.1 CDS serC NC_008611.1 276608 277738 D catalyzes the formation of 3-phosphonooxypyruvate and glutamate from O-phospho-L-serine and 2-oxoglutarate; phosphoserine aminotransferase 276608..277738 Mycobacterium ulcerans Agy99 4551603 YP_904473.1 CDS MUL_0267 NC_008611.1 277860 278651 D cytoplasmic protein; hypothetical protein 277860..278651 Mycobacterium ulcerans Agy99 4551604 YP_904474.1 CDS MUL_0268 NC_008611.1 278648 278935 R membrane protein; transmembrane protein complement(278648..278935) Mycobacterium ulcerans Agy99 4551605 YP_904475.1 CDS MUL_0269 NC_008611.1 278932 279738 R cytoplasmic protein; causes methylation.; rRNA methyltransferase complement(278932..279738) Mycobacterium ulcerans Agy99 4551606 YP_904476.1 CDS MUL_0270 NC_008611.1 279768 280199 R cytoplasmic protein; thought to be involved in transcriptional mechanism.; MarR family transcriptional regulator complement(279768..280199) Mycobacterium ulcerans Agy99 4551607 YP_904477.1 CDS MUL_0271 NC_008611.1 280458 280739 D membrane protein; transmembrane protein 280458..280739 Mycobacterium ulcerans Agy99 4551608 YP_904478.1 CDS MUL_0272 NC_008611.1 280763 281218 D Detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; hypothetical protein 280763..281218 Mycobacterium ulcerans Agy99 4551609 YP_904479.1 CDS MUL_0273 NC_008611.1 281215 282018 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; hypothetical protein complement(281215..282018) Mycobacterium ulcerans Agy99 4551610 YP_904480.1 CDS MUL_0274 NC_008611.1 282124 283833 D membrane protein; transmembrane protein 282124..283833 Mycobacterium ulcerans Agy99 4551611 YP_904481.1 CDS MUL_0275 NC_008611.1 283830 284318 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 283830..284318 Mycobacterium ulcerans Agy99 4551612 YP_904482.1 CDS MUL_0276 NC_008611.1 284426 285280 D cytoplasmic protein; hypothetical protein 284426..285280 Mycobacterium ulcerans Agy99 4551613 YP_904483.1 CDS MUL_0277 NC_008611.1 285277 286188 R cytoplasmic protein; hypothetical protein complement(285277..286188) Mycobacterium ulcerans Agy99 4551614 YP_904484.1 CDS fadE10 NC_008611.1 286293 288245 R cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE10 complement(286293..288245) Mycobacterium ulcerans Agy99 4551615 YP_904485.1 CDS cspB NC_008611.1 288432 288986 R cytoplasmic protein; function unknown, thought to act in response to low temperature.; cold shock-like protein B CspB complement(288432..288986) Mycobacterium ulcerans Agy99 4551616 YP_904486.1 CDS MUL_0280 NC_008611.1 289000 289404 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 289000..289404 Mycobacterium ulcerans Agy99 4551617 YP_904487.1 CDS moaA NC_008611.1 289401 290483 D together with moaC, is involved in the conversion of a guanosine derivative (GXP) into molybdopterin precursor Z; molybdenum cofactor biosynthesis protein A 289401..290483 Mycobacterium ulcerans Agy99 4551618 YP_904488.1 CDS moaD2 NC_008611.1 290497 290790 D cytoplasmic protein; involved in molybdenum cofactor biosynthesis.; molybdenum cofactor biosynthesis protein D 2 MoaD2 290497..290790 Mycobacterium ulcerans Agy99 4554350 YP_904489.1 CDS rpfA NC_008611.1 291203 292066 D membrane protein; function unknown. may be promote the resuscitation and growth of dormant, nongrowing cell.; resuscitation-promoting factor RpfA 291203..292066 Mycobacterium ulcerans Agy99 4554351 YP_904490.1 CDS moaE2 NC_008611.1 292111 292536 R cytoplasmic protein; possibly a molybdenum biosynthesis cofactor. conversion of molybdopterin precursor Z into molybdopterin requires transfer of two sulfur atoms to precursor Z (to generate the dithiolene group) this is catalyzed by the converting factor composed of a small and large subunit.; molybdenum cofactor biosynthesis protein E2 MoaE2 complement(292111..292536) Mycobacterium ulcerans Agy99 4554352 YP_904491.1 CDS mog NC_008611.1 292533 293018 R cytoplasmic protein; involved in molybdopterin biosynthesis; involved in the biosynthesis of a demolybdo-cofactor (molybdopterin), necessary for molybdo-enzymes.; molybdopterin biosynthesis Mog protein complement(292533..293018) Mycobacterium ulcerans Agy99 4554353 YP_904492.1 CDS moaC2 NC_008611.1 293015 293560 R cytoplasmic protein; involved in the biosynthesis of molybdopterin.; molybdenum cofactor biosynthesis protein C 2 MoaC2 complement(293015..293560) Mycobacterium ulcerans Agy99 4554354 YP_904493.1 CDS MUL_0287 NC_008611.1 293564 293773 R hypothetical protein complement(293564..293773) Mycobacterium ulcerans Agy99 4554355 YP_904494.1 CDS MUL_0288 NC_008611.1 293836 296094 D hypothetical protein 293836..296094 Mycobacterium ulcerans Agy99 4554356 YP_904495.1 CDS ercc3 NC_008611.1 296198 297826 D cytoplasmic protein; involved in nucleotide excision repair. has helicase activity: acts by opening DNA either around the RNA transcription start site or the DNA damage.; DNA helicase Ercc3 296198..297826 Mycobacterium ulcerans Agy99 4554357 YP_904496.1 CDS MUL_0290 NC_008611.1 297938 298375 R cytoplasmic protein; hypothetical protein complement(297938..298375) Mycobacterium ulcerans Agy99 4554358 YP_904497.1 CDS MUL_0291 NC_008611.1 298676 298996 D membrane protein; hypothetical protein 298676..298996 Mycobacterium ulcerans Agy99 4554359 YP_904498.1 CDS MUL_0292 NC_008611.1 299024 299971 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; function unknown, may have reductase activity; hypothetical protein complement(299024..299971) Mycobacterium ulcerans Agy99 4554360 YP_904499.1 CDS MUL_0293 NC_008611.1 300077 300616 R cytoplasmic protein; transcriptional regulator complement(300077..300616) Mycobacterium ulcerans Agy99 4554361 YP_904500.1 CDS MUL_0294 NC_008611.1 300693 301904 D Also detected in the cytoplasmic fraction.; membrane protein; membrane-associated oxidoreductase 300693..301904 Mycobacterium ulcerans Agy99 4554362 YP_904501.1 CDS fadB NC_008611.1 301919 304063 R Detected in the membrane fraction by proteomics (LC- MS/MS) Also detected in the extracellular matrix by proteomics.; membrane protein; involved in fatty acid degradation (probably in fatty acid beta-oxidation cycle); fatty oxidation protein FadB complement(301919..304063) Mycobacterium ulcerans Agy99 4554363 YP_904502.1 CDS fadA NC_008611.1 304068 305279 R Catalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation; acetyl-CoA acetyltransferase complement(304068..305279) Mycobacterium ulcerans Agy99 4554364 YP_904503.1 CDS MUL_0297 NC_008611.1 305484 306680 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; aminotransferase 305484..306680 Mycobacterium ulcerans Agy99 4554365 YP_904504.1 CDS MUL_0299 NC_008611.1 307165 307620 R cytoplasmic protein; hypothetical protein complement(307165..307620) Mycobacterium ulcerans Agy99 4554367 YP_904505.1 CDS MUL_0301 NC_008611.1 308797 309237 R cytoplasmic protein; conserved hypothetical protein; hypothetical protein complement(308797..309237) Mycobacterium ulcerans Agy99 4554369 YP_904506.1 CDS pdc NC_008611.1 309301 311001 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; possible indole-3-pyruvate decarboxylase; [catalytic activity: 3-(indol-3-YL) pyruvate = 2-(indol-3- YL)acetaldehyde + CO2], or possible pyruvate decarboxylase; [catalytic activity: a 2-oxo acid = an aldehyde + CO2]; pyruvate or indole-3-pyruvate decarboxylase Pdc 309301..311001 Mycobacterium ulcerans Agy99 4549594 YP_904507.1 CDS MUL_0303 NC_008611.1 311016 311201 R cytoplasmic protein; conserved hypothetical protein; hypothetical protein complement(311016..311201) Mycobacterium ulcerans Agy99 4549595 YP_904508.1 CDS MUL_0305 NC_008611.1 311874 312467 R membrane protein; hypothetical protein complement(311874..312467) Mycobacterium ulcerans Agy99 4549597 YP_904509.1 CDS MUL_0306 NC_008611.1 312464 313366 R membrane protein; hypothetical protein complement(312464..313366) Mycobacterium ulcerans Agy99 4549598 YP_904510.1 CDS MUL_0308 NC_008611.1 315163 316344 R cytoplasmic protein; function unknown, contains a hydrolytic domain.; hypothetical protein complement(315163..316344) Mycobacterium ulcerans Agy99 4549600 YP_904511.1 CDS MUL_0309 NC_008611.1 316438 316860 R membrane protein; lipoprotein complement(316438..316860) Mycobacterium ulcerans Agy99 4549601 YP_904512.1 CDS echA8_2 NC_008611.1 317004 317768 D cytoplasmic protein; oxidizes fatty acids using specific components (by similarity) [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]; enoyl-CoA hydratase, EchA8_2 317004..317768 Mycobacterium ulcerans Agy99 4549602 YP_904513.1 CDS MUL_0311 NC_008611.1 317765 318196 D secreted protein; hypothetical protein 317765..318196 Mycobacterium ulcerans Agy99 4549603 YP_904514.1 CDS MUL_0313 NC_008611.1 318774 319097 D cytoplasmic protein; hypothetical protein 318774..319097 Mycobacterium ulcerans Agy99 4549606 YP_904515.1 CDS cyp150A6 NC_008611.1 319116 320390 R cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.; cytochrome P450 150A6 Cyp150A6 complement(319116..320390) Mycobacterium ulcerans Agy99 4549607 YP_904516.1 CDS fabG2 NC_008611.1 320674 321405 D membrane protein; involved in the fatty acid biosynthesis [catalytic activity: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH]; 3-oxoacyl-ACP reductase 320674..321405 Mycobacterium ulcerans Agy99 4549608 YP_904517.1 CDS MUL_0316 NC_008611.1 321514 321714 D cytoplasmic protein; ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.; ferredoxin 321514..321714 Mycobacterium ulcerans Agy99 4549609 YP_904518.1 CDS cyp188A3 NC_008611.1 321729 323141 D cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.; cytochrome P450 188A3 Cyp188A3 321729..323141 Mycobacterium ulcerans Agy99 4549610 YP_904519.1 CDS MUL_0319 NC_008611.1 324354 325394 R cytoplasmic protein; function unknown, but supposed involvement in lipid degradation.; acyl-CoA dehydrogenase complement(324354..325394) Mycobacterium ulcerans Agy99 4549612 YP_904520.1 CDS MUL_0320 NC_008611.1 325454 325954 D cytoplasmic protein; hypothetical protein 325454..325954 Mycobacterium ulcerans Agy99 4552639 YP_904521.1 CDS MUL_0321 NC_008611.1 326429 327964 R membrane protein; function unknown, possibly involved in transport of sulfate across the membrane.; integral membrane transporter complement(326429..327964) Mycobacterium ulcerans Agy99 4552640 YP_904522.1 CDS MUL_0322 NC_008611.1 328370 329452 R cytoplasmic protein; function unknown, but supposed involvement in lipid degradation.; acyl-CoA dehydrogenase complement(328370..329452) Mycobacterium ulcerans Agy99 4552641 YP_904523.1 CDS MUL_0323 NC_008611.1 329643 330296 D cytoplasmic protein; could be involved in transcriptional mechanism.; transcriptional regulatory protein 329643..330296 Mycobacterium ulcerans Agy99 4552642 YP_904524.1 CDS cyp189A7 NC_008611.1 330530 331732 D cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.; cytochrome P450 189A7 Cyp189A7 330530..331732 Mycobacterium ulcerans Agy99 4552643 YP_904525.1 CDS MUL_0326 NC_008611.1 332625 333455 D cytoplasmic protein; function unknown, possibly involved in cellular metabolism.; short chain dehydrogenase 332625..333455 Mycobacterium ulcerans Agy99 4552645 YP_904526.1 CDS MUL_0327 NC_008611.1 333452 334258 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase complement(333452..334258) Mycobacterium ulcerans Agy99 4552646 YP_904527.1 CDS MUL_0328 NC_008611.1 334267 336705 R cytoplasmic protein; acyl-CoA transferase complement(334267..336705) Mycobacterium ulcerans Agy99 4552647 YP_904528.1 CDS MUL_0330 NC_008611.1 337700 338818 R membrane protein; function unknown, hydrolyses peptides.; aminopeptidase complement(337700..338818) Mycobacterium ulcerans Agy99 4552649 YP_904529.1 CDS MUL_0331 NC_008611.1 338808 339977 R cytoplasmic protein; hydrolysis of peptide bonds; dipeptidase complement(338808..339977) Mycobacterium ulcerans Agy99 4552650 YP_904530.1 CDS cyp105Q4 NC_008611.1 341262 342506 R cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.; cytochrome P450 105Q4 Cyp105Q4 complement(341262..342506) Mycobacterium ulcerans Agy99 4552652 YP_904531.1 CDS MUL_0334 NC_008611.1 342509 342703 R cytoplasmic protein; ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.; ferredoxin complement(342509..342703) Mycobacterium ulcerans Agy99 4552653 YP_904532.1 CDS MUL_0335 NC_008611.1 342716 343381 R cytoplasmic protein; function unknown, maybe a transcriptional regulator; hypothetical protein complement(342716..343381) Mycobacterium ulcerans Agy99 4552654 YP_904533.1 CDS MUL_0336 NC_008611.1 343471 344274 R cytoplasmic protein; short chain dehydrogenase complement(343471..344274) Mycobacterium ulcerans Agy99 4552655 YP_904534.1 CDS MUL_0338 NC_008611.1 345276 346553 R membrane protein; thought to be involved in transport of undeterminated substrate (possibly drug) across the membrane. responsible for the translocation of the substrate across the membrane.; integral membrane transport protein complement(345276..346553) Mycobacterium ulcerans Agy99 4552657 YP_904535.1 CDS lpqS NC_008611.1 347877 348293 R membrane protein; lipoprotein LpqS complement(347877..348293) Mycobacterium ulcerans Agy99 4550871 YP_904536.1 CDS MUL_0341 NC_008611.1 348414 350072 D Also detected in the cytoplasmic and the membrane fraction by proteomics..; membrane protein; probable oxidase, showing similarity with several oxidases, mainly L-ascorbate oxidases and copper resistance proteins; oxidase 348414..350072 Mycobacterium ulcerans Agy99 4550872 YP_904537.1 CDS MUL_0342 NC_008611.1 350103 350921 R Transmembrane helix. Detected in the membrane and the extracellular fractions by proteomics; membrane protein; function unknown, predicted hydrolase or acyltransferase domain.; hypothetical protein complement(350103..350921) Mycobacterium ulcerans Agy99 4550873 YP_904538.1 CDS MUL_0343 NC_008611.1 350921 351913 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS) Also detected in the cytoplamic fraction by LC- MS/MS; cytoplasmic protein; putative cyclase; hypothetical protein complement(350921..351913) Mycobacterium ulcerans Agy99 4550874 YP_904539.1 CDS MUL_0344 NC_008611.1 351910 352725 R catalyzes the formation of 5-alpha-androstane-3,17-dione from androsterone; Acts on other 3-alpha-hydroxysteroids and on 9-, 11- and 15-hydroxyprostaglandin.; 3-alpha-hydroxysteroid dehydrogenase complement(351910..352725) Mycobacterium ulcerans Agy99 4550875 YP_904540.1 CDS MUL_0345 NC_008611.1 352760 354061 R cytoplasmic protein; monoxygenase complement(352760..354061) Mycobacterium ulcerans Agy99 4550876 YP_904541.1 CDS MUL_0346 NC_008611.1 354064 355818 R initiates steroid ring degradation; catalyzes the transhydrogenation of 3-keto-4-ene-steroid to 3-keto-1,4-diene-steroid e.g., progesterone to 1,4-androstadiene-3,17-dione; 3-ketosteroid-delta-1-dehydrogenase complement(354064..355818) Mycobacterium ulcerans Agy99 4550877 YP_904542.1 CDS mhpB NC_008611.1 355815 356645 R catalyzes the cleavage of 3-(2,3-dihydroxyphenyl) propionate into 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate; part of the 3-phenylpropionic acid degradation pathway; member of the protocatechuate 4,5-dioxygenase family; 3-(2,3-dihydroxyphenyl)propionate dioxygenase complement(355815..356645) Mycobacterium ulcerans Agy99 4550878 YP_904543.1 CDS MUL_0348 NC_008611.1 357322 357999 D cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein 357322..357999 Mycobacterium ulcerans Agy99 4550879 YP_904544.1 CDS MUL_0349 NC_008611.1 358017 359627 D membrane protein; dehydrogenase 358017..359627 Mycobacterium ulcerans Agy99 4550880 YP_904545.1 CDS MUL_0350 NC_008611.1 359634 361028 R membrane protein; possible sensor part of a two component regulatory system; two component sensor kinase complement(359634..361028) Mycobacterium ulcerans Agy99 4550881 YP_904546.1 CDS narL NC_008611.1 361076 361726 D cytoplasmic protein; nitrate/nitrite response regulator protein NarL 361076..361726 Mycobacterium ulcerans Agy99 4550882 YP_904547.1 CDS MUL_0354 NC_008611.1 364334 365872 R membrane protein; integral membrane protein complement(364334..365872) Mycobacterium ulcerans Agy99 4550885 YP_904548.1 CDS MUL_0355 NC_008611.1 365985 366284 R membrane protein; PE-PGRS family protein complement(365985..366284) Mycobacterium ulcerans Agy99 4550886 YP_904549.1 CDS MUL_0356 NC_008611.1 366373 367251 R membrane protein; transport system kinase - possibly arginine; transport system kinase complement(366373..367251) Mycobacterium ulcerans Agy99 4550887 YP_904550.1 CDS MUL_0357 NC_008611.1 367269 368468 R cytoplasmic protein; function unknown but may be involved in fatty acid oxidation (BetA oxidation); beta-ketoacyl CoA thiolase complement(367269..368468) Mycobacterium ulcerans Agy99 4550888 YP_904551.1 CDS pip NC_008611.1 368579 369409 D cytoplasmic protein; specifically catalyzes the removal of N-terminal proline residues from peptides. thought to release the N- terminal proline from the dipeptides, pro-pro, pro-gln, pro-trp and pro-tyr; also from amides (pro-beta na) and oligopeptides, pro-leu-glynH2, pro-leu-gly and pro-phe-gly- lys. higher activity toward small peptides (up to three residues), but very low activity for longer peptides [catalytic activity: release of a N-terminal proline from a peptide]; proline iminopeptidase PIP 368579..369409 Mycobacterium ulcerans Agy99 4554388 YP_904552.1 CDS MUL_0359 NC_008611.1 369438 370205 R cytoplasmic protein; lipid metabolism; enoyl-CoA hydratase complement(369438..370205) Mycobacterium ulcerans Agy99 4554389 YP_904553.1 CDS MUL_0360 NC_008611.1 370207 371097 R cytoplasmic protein; involved in lipid metabolism.; acyl-CoA dehydrogenase complement(370207..371097) Mycobacterium ulcerans Agy99 4554390 YP_904554.1 CDS MUL_0361 NC_008611.1 371291 372160 R cytoplasmic protein; involved in lipid metabolism.; acyl-CoA dehydrogenase complement(371291..372160) Mycobacterium ulcerans Agy99 4554391 YP_904555.1 CDS MUL_0362 NC_008611.1 372175 373710 R cytoplasmic protein; acyl-CoA synthetase complement(372175..373710) Mycobacterium ulcerans Agy99 4554392 YP_904556.1 CDS MUL_0363 NC_008611.1 373694 373975 D cytoplasmic protein; similar to ferredoxin reductase electron transfer proteins; electron transfer protein 373694..373975 Mycobacterium ulcerans Agy99 4554393 YP_904557.1 CDS MUL_0364 NC_008611.1 374075 375046 R cytoplasmic protein; acyl-CoA transferase complement(374075..375046) Mycobacterium ulcerans Agy99 4554394 YP_904558.1 CDS MUL_0365 NC_008611.1 375043 375999 R cytoplasmic protein; oxidoreductase complement(375043..375999) Mycobacterium ulcerans Agy99 4554395 YP_904559.1 CDS mcmA2b NC_008611.1 375984 376421 R cytoplasmic protein; involved in propionic acid fermentation. catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid- cycle intermediates [catalytic activity : (R)-2-methyl-3- oxopropanoyl-CoA = succinyl- CoA]; methylmalonyl-CoA mutase subunit alpha, McmA2b complement(375984..376421) Mycobacterium ulcerans Agy99 4554396 YP_904560.1 CDS mcmA2a NC_008611.1 376422 377999 R cytoplasmic protein; catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates [catalytic activity : (R)-2-methyl-3-oxopropanoyl-CoA = succinyl- CoA] the enzyme methylmalonyl-CoA mutase is a member of a class of enzymes that uses coenzyme B12 (adenosylcobalamin) as a cofactor. the enzyme induces the formation of an adenosyl radical from the cofactor. this radical then initiates a free-radical rearrangement of its substrate, succinyl-CoA, to methylmalonyl-CoA.; methylmalonyl-CoA mutase beta subunit, McmA2a complement(376422..377999) Mycobacterium ulcerans Agy99 4554397 YP_904561.1 CDS MUL_0368 NC_008611.1 378125 378937 R cytoplasmic protein; possible methylase; hypothetical protein complement(378125..378937) Mycobacterium ulcerans Agy99 4554398 YP_904562.1 CDS lpqR NC_008611.1 378998 379807 R membrane protein; lipoprotein LpqR complement(378998..379807) Mycobacterium ulcerans Agy99 4554399 YP_904563.1 CDS MUL_0370 NC_008611.1 379918 380811 R cytoplasmic protein; transposase for IS2404; transposase for IS2404 complement(379918..380811) Mycobacterium ulcerans Agy99 4554400 YP_904564.1 CDS MUL_0371 NC_008611.1 381870 382724 D secreted protein; function unknown, contains a N-term cellobiohydrolase a (1,4-beta-cellobiosidase a) domain and a C-term cellulose-binding domain (CBD) domain. the CBD is found either at the N-term or at the C-terminal extremity of endoglucanases, cellobiohydrolases (exoglucanases), or xylanases; chitinase/cellulase 381870..382724 Mycobacterium ulcerans Agy99 4554402 YP_904565.1 CDS MUL_0372 NC_008611.1 382743 383321 R membrane protein; hypothetical protein complement(382743..383321) Mycobacterium ulcerans Agy99 4554403 YP_904566.1 CDS crtE NC_008611.1 383720 384712 D cytoplasmic protein; converts farensyl pyrophosphate to geranylgeranyl pyrophosphate, the latter being the substrate for CrtB in the production of phytoene during carotenoid synthesis; geranylgeranyl pyrophosphate synthase 383720..384712 Mycobacterium ulcerans Agy99 4554404 YP_904567.1 CDS crtB NC_008611.1 386470 387429 D cytoplasmic protein; part of carotenoid biosynthesis cluster - photochromogenesis; phytoene synthase, CrtB 386470..387429 Mycobacterium ulcerans Agy99 4554406 YP_904568.1 CDS crtYc NC_008611.1 387426 387749 D membrane protein; involved in carotenoid biosynthesis; lycopene cyclase, CrtYc 387426..387749 Mycobacterium ulcerans Agy99 4552709 YP_904569.1 CDS crtYd NC_008611.1 387746 388120 D cytoplasmic protein; involved in carotenoid biosynthesis; lycopene cyclase, CrtYd 387746..388120 Mycobacterium ulcerans Agy99 4552710 YP_904570.1 CDS MUL_0379 NC_008611.1 390469 391020 D cytoplasmic protein; involved in transcriptional mechanism.; MarR family transcriptional regulator 390469..391020 Mycobacterium ulcerans Agy99 4552712 YP_904571.1 CDS idi2 NC_008611.1 391030 392076 R catalyzes the isomerization of isopentenyl pyrophosphate to dimethylallyl diphosphate; isopentenyl pyrophosphate isomerase complement(391030..392076) Mycobacterium ulcerans Agy99 4552713 YP_904572.1 CDS MUL_0381 NC_008611.1 392191 392898 R cytoplasmic protein; hypothetical protein complement(392191..392898) Mycobacterium ulcerans Agy99 4552714 YP_904573.1 CDS MUL_0382 NC_008611.1 392892 393494 R membrane protein; hypothetical protein complement(392892..393494) Mycobacterium ulcerans Agy99 4552715 YP_904574.1 CDS ansP1 NC_008611.1 393576 395060 R membrane protein; involved in L-asparagine transport; L-asparagine permease AnsP1 complement(393576..395060) Mycobacterium ulcerans Agy99 4552716 YP_904575.1 CDS MUL_0384 NC_008611.1 395104 395346 R membrane protein; hypothetical protein complement(395104..395346) Mycobacterium ulcerans Agy99 4552717 YP_904576.1 CDS MUL_0385 NC_008611.1 395662 396096 R membrane protein; function unknown, predicted ATPase domain; hypothetical protein complement(395662..396096) Mycobacterium ulcerans Agy99 4552718 YP_904577.1 CDS MUL_0387 NC_008611.1 397144 398190 D cytoplasmic protein; transposase for IS2404; transposase for IS2404 397144..398190 Mycobacterium ulcerans Agy99 4552720 YP_904578.1 CDS MUL_0389 NC_008611.1 399429 400643 D cytoplasmic protein; contains amidohydrolase domain; hypothetical protein 399429..400643 Mycobacterium ulcerans Agy99 4552722 YP_904579.1 CDS MUL_0391 NC_008611.1 402249 403910 D membrane protein; PE-PGRS family protein 402249..403910 Mycobacterium ulcerans Agy99 4552724 YP_904580.1 CDS cspA NC_008611.1 404179 404382 R Detected in the secreted fraction by 2D-LC-MS/MS.; secreted protein; possibly involved in cold acclimatization processes (the production of the protein is supposed predominantly induced at low temperatures); cold shock protein a, CspA complement(404179..404382) Mycobacterium ulcerans Agy99 4552725 YP_904581.1 CDS rhlE1 NC_008611.1 404475 406049 R cytoplasmic protein; function unknown, possibly involved in ATP- dependent RNA unwinding, enzymes of this class are needed in a variety of cellular processes including splicing, ribosome biogenesis and RNA degradation.; ATP-dependent RNA helicase, RhlE1 complement(404475..406049) Mycobacterium ulcerans Agy99 4552726 YP_904582.1 CDS MUL_0394 NC_008611.1 406250 406399 D cytoplasmic protein; hypothetical protein 406250..406399 Mycobacterium ulcerans Agy99 4553945 YP_904583.1 CDS metH NC_008611.1 406789 410622 D one of two methionine synthases in Escherichia coli; MetH catalyzes a methyl transfer reaction from methyltetrahydrofolate to homocysteine to create methionine; requires zinc for activity; B12-dependent methionine synthase 406789..410622 Mycobacterium ulcerans Agy99 4553946 YP_904584.1 CDS MUL_0396 NC_008611.1 411028 411759 D cytoplasmic protein; contains a nucleotidyltransferase domain; hypothetical protein 411028..411759 Mycobacterium ulcerans Agy99 4553947 YP_904585.1 CDS MUL_0397 NC_008611.1 411698 412234 D cytoplasmic protein; hypothetical protein 411698..412234 Mycobacterium ulcerans Agy99 4553948 YP_904586.1 CDS MUL_0398 NC_008611.1 412514 413560 D cytoplasmic protein; transposase for IS2404; transposase for IS2404 412514..413560 Mycobacterium ulcerans Agy99 4553949 YP_904587.1 CDS MUL_0399 NC_008611.1 413626 414087 R membrane protein; PPE family protein complement(413626..414087) Mycobacterium ulcerans Agy99 4553950 YP_904588.1 CDS MUL_0400 NC_008611.1 414410 414655 D With PurL and PurQ catalyzes the conversion of formylglycinamide ribonucleotide, ATP, and glutamine to formylglycinamidine ribonucleotide, ADP, and glutamate in the fourth step of the purine biosynthetic pathway; phosphoribosylformylglycinamidine synthase subunit PurS 414410..414655 Mycobacterium ulcerans Agy99 4553951 YP_904589.1 CDS purQ NC_008611.1 414652 415326 D catalyzes the formation of 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and L-glutamine in purine biosynthesis; phosphoribosylformylglycinamidine synthase I 414652..415326 Mycobacterium ulcerans Agy99 4553952 YP_904590.1 CDS MUL_0402 NC_008611.1 415338 415802 R cytoplasmic protein; hypothetical protein complement(415338..415802) Mycobacterium ulcerans Agy99 4553953 YP_904591.1 CDS MUL_0403 NC_008611.1 415903 416367 R cytoplasmic protein; contains mannose-6-phosphate isomerase [carbohydrate transport and metabolism]; hypothetical protein complement(415903..416367) Mycobacterium ulcerans Agy99 4553954 YP_904592.1 CDS lpdB NC_008611.1 416393 417925 R cytoplasmic protein; contains pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component domain; dihydrolipoamide dehydrogenase, LpdB complement(416393..417925) Mycobacterium ulcerans Agy99 4553955 YP_904593.1 CDS cfp29 NC_008611.1 418436 419242 R Also detected in the extracellular matrix and membrane fraction by proteomics.; secreted protein; function unknown, high domain homology to Linocin_M18, Linocin_M18 bacteriocin protein. the Linocin_M18 region is found mostly in eubacteria, though homologous sequences have been identified in archaea; 29 kDa antigen Cfp29 complement(418436..419242) Mycobacterium ulcerans Agy99 4553956 YP_904594.1 CDS MUL_0406 NC_008611.1 419239 420246 R membrane protein; contains Dyp-type peroxidase domain. this family of dye-decolourising peroxidases lack a typical heme-binding region; hypothetical protein complement(419239..420246) Mycobacterium ulcerans Agy99 4553957 YP_904595.1 CDS MUL_0409 NC_008611.1 422006 422656 R cytoplasmic protein; domain similarity with acetyltransferases; hypothetical protein complement(422006..422656) Mycobacterium ulcerans Agy99 4553960 YP_904596.1 CDS purL NC_008611.1 422754 425051 D catalyzes the formation of 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and L-glutamine in purine biosynthesis; phosphoribosylformylglycinamidine synthase II 422754..425051 Mycobacterium ulcerans Agy99 4553961 YP_904597.1 CDS MUL_0411 NC_008611.1 425084 425698 D secreted protein; contains CAAX amino terminal protease domain. members of this family are probably proteases; the family contains CAAX prenyl protease. the proteins contain a highly conserved Glu-Glu motif at the amino End of the alignment. the alignment also contains two histidine residues that may be involved in zinc binding; hypothetical protein 425084..425698 Mycobacterium ulcerans Agy99 4553962 YP_904598.1 CDS MUL_0412 NC_008611.1 425738 426706 D cytoplasmic protein; contains phosphohydrolase domain; hypothetical protein 425738..426706 Mycobacterium ulcerans Agy99 4553963 YP_904599.1 CDS cpsY NC_008611.1 426651 428249 R cytoplasmic protein; thought to be involved in exopolysaccharide and/or lipopolysaccharide biosynthetic pathway [catalytic activity: UDP-glucose = UDP-galactose]; UDP-glucose 4-epimerase complement(426651..428249) Mycobacterium ulcerans Agy99 4551766 YP_904600.1 CDS mce2A NC_008611.1 429782 430987 R membrane protein; MCE-family protein Mce2A complement(429782..430987) Mycobacterium ulcerans Agy99 4551768 YP_904601.1 CDS MUL_0416 NC_008611.1 431007 431396 D cytoplasmic protein; hypothetical protein 431007..431396 Mycobacterium ulcerans Agy99 4551769 YP_904602.1 CDS purF NC_008611.1 431421 433061 D Catalyzes first step of the de novo purine nucleotide biosynthetic pathway; amidophosphoribosyltransferase 431421..433061 Mycobacterium ulcerans Agy99 4551770 YP_904603.1 CDS purM NC_008611.1 433065 434228 D catalyzes the formation of 1-(5-phosphoribosyl)-5-aminoimidazole from 2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in purine biosynthesis; phosphoribosylaminoimidazole synthetase 433065..434228 Mycobacterium ulcerans Agy99 4551771 YP_904604.1 CDS MUL_0419 NC_008611.1 434279 434458 R cytoplasmic protein; hypothetical protein complement(434279..434458) Mycobacterium ulcerans Agy99 4551772 YP_904605.1 CDS MUL_0420 NC_008611.1 434617 435708 R cytoplasmic protein; contains predicted aminomethyltransferase domain, related to GcvT - involved in the catabolism of glycine; hypothetical protein complement(434617..435708) Mycobacterium ulcerans Agy99 4551773 YP_904606.1 CDS ctpV NC_008611.1 436343 438676 R membrane protein; metal cation-transporting ATPase; possibly catalyzes the transport of undetermined metal cation with hydrolyse of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation (out)]; metal cation transporter p-type ATPase, CtpV complement(436343..438676) Mycobacterium ulcerans Agy99 4551775 YP_904607.1 CDS MUL_0424 NC_008611.1 438732 439040 R cytoplasmic protein; hypothetical protein complement(438732..439040) Mycobacterium ulcerans Agy99 4551776 YP_904608.1 CDS MUL_0425 NC_008611.1 439142 439528 R cytoplasmic protein; hypothetical protein complement(439142..439528) Mycobacterium ulcerans Agy99 4551777 YP_904609.1 CDS pabC NC_008611.1 439630 440520 D catalyzes the formation of 4-aminobenzoate and pyruvate from 4-amino-4-deoxychorismate; 4-amino-4-deoxychorismate lyase 439630..440520 Mycobacterium ulcerans Agy99 4551778 YP_904610.1 CDS MUL_0429 NC_008611.1 443275 443859 R cytoplasmic protein; N-terminal truncated in relation to orthologs; hypothetical protein complement(443275..443859) Mycobacterium ulcerans Agy99 4551780 YP_904611.1 CDS MUL_0430 NC_008611.1 443942 444040 R membrane protein; no H37Rv ortholog - very small, doubtful ORF; hypothetical protein complement(443942..444040) Mycobacterium ulcerans Agy99 4551781 YP_904612.1 CDS sseC2 NC_008611.1 444097 444399 R cytoplasmic protein; sulfur metabolism protein, SseC2 complement(444097..444399) Mycobacterium ulcerans Agy99 4551782 YP_904613.1 CDS cysA2 NC_008611.1 444401 445243 R Also detected in the membrane fraction by proteomics.; cytoplasmic protein; may be a sulfotransferase involved in the formation of thiosulfate [catalytic activity: thiosulfate + cyanide = sulfite + thiocyanate]; thiosulfate sulfurtransferase, CysA2 complement(444401..445243) Mycobacterium ulcerans Agy99 4551783 YP_904614.1 CDS MUL_0433 NC_008611.1 445281 445748 R membrane protein; integral membrane protein complement(445281..445748) Mycobacterium ulcerans Agy99 4551784 YP_904615.1 CDS MUL_0434 NC_008611.1 446048 446860 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(446048..446860) Mycobacterium ulcerans Agy99 4549973 YP_904616.1 CDS MUL_0435 NC_008611.1 447264 448031 D cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein 447264..448031 Mycobacterium ulcerans Agy99 4549974 YP_904617.1 CDS mshD NC_008611.1 448028 448990 D cytoplasmic protein; involved in the fourth step of mycothiol biosynthesis; mycothiol acetyltransferase, MshD 448028..448990 Mycobacterium ulcerans Agy99 4549975 YP_904618.1 CDS phoS3 NC_008611.1 449127 450233 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in phosphate transport; phosphate-binding protein 3 , PhoS3 449127..450233 Mycobacterium ulcerans Agy99 4549976 YP_904619.1 CDS pstA NC_008611.1 451319 452227 D membrane protein; nvolved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. this is one of the proteins required for binding-protein- mediated phosphate transport.; phosphate-transport integral membrane ABC transporter, PstA 451319..452227 Mycobacterium ulcerans Agy99 4549978 YP_904620.1 CDS phoT NC_008611.1 452247 453023 D ATP-binding protein; PstABCS is an ATP dependent phosphate uptake system which is responsible for inorganic phosphate uptake during phosphate starvation; phosphate transporter ATP-binding protein 452247..453023 Mycobacterium ulcerans Agy99 4549979 YP_904621.1 CDS phoY2 NC_008611.1 453058 453726 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); cytoplasmic protein; involved in transcriptional regulation of active transport of inorganic phosphate across the membrane; phosphate-transport system regulatory protein, PhoY2 complement(453058..453726) Mycobacterium ulcerans Agy99 4549980 YP_904622.1 CDS MUL_0444 NC_008611.1 456174 457307 R cytoplasmic protein; domain homology - tRNA-dihydrouridine synthase [translation, ribosomal structure and biogenesis]; transcriptional regulator complement(456174..457307) Mycobacterium ulcerans Agy99 4549982 YP_904623.1 CDS desA1 NC_008611.1 457316 458332 R cytoplasmic protein; involved in mycolic acid biosynthesis; acyl-[acyl-carrier protein] desaturase DesA1 complement(457316..458332) Mycobacterium ulcerans Agy99 4549983 YP_904624.1 CDS MUL_0446 NC_008611.1 458500 459141 R membrane protein; hypothetical protein complement(458500..459141) Mycobacterium ulcerans Agy99 4549984 YP_904625.1 CDS MUL_0447 NC_008611.1 459215 460273 D cytoplasmic protein; hypothetical protein 459215..460273 Mycobacterium ulcerans Agy99 4549985 YP_904626.1 CDS MUL_0448 NC_008611.1 460485 460856 R cytoplasmic protein; transcription regulator ArsR complement(460485..460856) Mycobacterium ulcerans Agy99 4549987 YP_904627.1 CDS MUL_0449 NC_008611.1 460914 461222 R cytoplasmic protein; function unknown, domain homology to CumB, cytosine/adenosine deaminases.; hypothetical protein complement(460914..461222) Mycobacterium ulcerans Agy99 4549988 YP_904628.1 CDS MUL_0450 NC_008611.1 461316 462221 D membrane protein; function unknown, may be involved in polyketide biosynthesis [secondary metabolites biosynthesis, transport, and catabolism]; O-methyltransferase 461316..462221 Mycobacterium ulcerans Agy99 4549989 YP_904629.1 CDS MUL_0452 NC_008611.1 463178 464224 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 463178..464224 Mycobacterium ulcerans Agy99 4552776 YP_904630.1 CDS MUL_0453 NC_008611.1 464190 465446 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; domain: regulator of polyketide synthase expression; hypothetical protein complement(464190..465446) Mycobacterium ulcerans Agy99 4552777 YP_904631.1 CDS atsD NC_008611.1 465476 467974 D membrane protein; thought to play an important role in the mineralization of sulfates [catalytic activity: a phenol sulfate + H2O = a phenol + sulfate]; arylsulfatase AtsD 465476..467974 Mycobacterium ulcerans Agy99 4552778 YP_904632.1 CDS MUL_0456 NC_008611.1 469958 470461 D cytoplasmic protein; putative OHCU decarboxylase 469958..470461 Mycobacterium ulcerans Agy99 4552780 YP_904633.1 CDS MUL_0457 NC_008611.1 470448 470819 D cytoplasmic protein; domain homology: transthyretin, transthyretin precursor (formerly prealbumin) transthyretin is a thyroid hormone-binding protein that transports thyroxine from the bloodstream to the brain. mutations in the human transthyretin are associated with several genetic disorders; hypothetical protein 470448..470819 Mycobacterium ulcerans Agy99 4552781 YP_904634.1 CDS MUL_0458 NC_008611.1 470635 471642 D membrane protein; domain: xanthine dehydrogenase, iron-sulfur cluster and FAD-binding subunit A [nucleotide transport and metabolism]; hypothetical protein 470635..471642 Mycobacterium ulcerans Agy99 4552782 YP_904635.1 CDS MUL_0459 NC_008611.1 471639 474341 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; carbon monoxide dehydrogenase 471639..474341 Mycobacterium ulcerans Agy99 4552783 YP_904636.1 CDS MUL_0460 NC_008611.1 474330 475259 R cytoplasmic protein; function unknown, contains a UbiH domain, 2- polyprenyl-6-methoxyphenol hydroxylase and related FAD- dependent oxidoreductases [coenzyme metabolism / energy production and conversion]; hypothetical protein complement(474330..475259) Mycobacterium ulcerans Agy99 4552784 YP_904637.1 CDS MUL_0462 NC_008611.1 475563 476291 R Detected in the membrane fraction by proteomics.; membrane protein; hypothetical protein complement(475563..476291) Mycobacterium ulcerans Agy99 4552786 YP_904638.1 CDS phoP NC_008611.1 476453 477175 D Detected in the membrane fraction by proteomics; membrane protein; involved in transcriptional mechanism. part of the two component regulatory system PhoP/PhoQ. this protein is thought to be a positive regulator for the phosphate regulon, required for intracellular growth. transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited; two component system response phosphate regulon transcriptional regulator, PhoP 476453..477175 Mycobacterium ulcerans Agy99 4552787 YP_904639.1 CDS phoR NC_008611.1 477216 478661 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; sensor part of a two component regulatory system. this protein is thought to be a sensor kinase for the phosphate regulon. transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited; two component system response phosphate sensor kinase, PhoR 477216..478661 Mycobacterium ulcerans Agy99 4552788 YP_904640.1 CDS MUL_0465 NC_008611.1 478668 479072 R Detected in the extracellular matrix by proteomics. Also detected in the cytoplasmic fraction by 2D-LC-MS/MS.; cytoplasmic protein; Hit family protein: diadenosine tetraphosphate (Ap4A) hydrolase and other Hit family hydrolases [nucleotide transport and metabolism / carbohydrate transport and metabolism / general function prediction only]; hypothetical protein complement(478668..479072) Mycobacterium ulcerans Agy99 4552789 YP_904641.1 CDS MUL_0467 NC_008611.1 480897 482339 D secreted protein; hypothetical protein 480897..482339 Mycobacterium ulcerans Agy99 4552791 YP_904642.1 CDS MUL_0468 NC_008611.1 482341 482760 R cytoplasmic protein; contains nuclear transport factor 2 (NTF2) domain; hypothetical protein complement(482341..482760) Mycobacterium ulcerans Agy99 4552792 YP_904643.1 CDS MUL_0469 NC_008611.1 482763 483047 R cytoplasmic protein; function unknown, domain homology suggests possible NADH:flavin oxidoreductase; hypothetical protein complement(482763..483047) Mycobacterium ulcerans Agy99 4552793 YP_904644.1 CDS adhB NC_008611.1 483077 484204 R membrane protein; thought to catalyze the reversible oxidation of ethanol to acetaldehyde with the concomitant reduction of NAD. probably acts on primary or secondary alcohols or hemiacetals [catalytic activity: an alcohol + NAD+ = an aldehyde or ketone + NADH]; zinc-containing alcohol dehydrogenase NAD- dependent AdhB complement(483077..484204) Mycobacterium ulcerans Agy99 4550676 YP_904645.1 CDS MUL_0471 NC_008611.1 484367 484912 R cytoplasmic protein; hypothetical protein complement(484367..484912) Mycobacterium ulcerans Agy99 4550677 YP_904646.1 CDS MUL_0472 NC_008611.1 484918 485124 R cytoplasmic protein; ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. probably involved in electron transport for cytochrome P- 450 system.; ferredoxin complement(484918..485124) Mycobacterium ulcerans Agy99 4550678 YP_904647.1 CDS cyp51B1 NC_008611.1 485124 486494 R cytoplasmic protein; involved in sterol biosynthesis. its biological substrate is not known. catalyzes C14-demethylation of lanosterol, 24,25-dihydrolanosterol and obtusifoliol which is critical for ergosterol biosynthesis. it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3- beta-ol.; cytochrome P450 51B1 Cyp51B1 complement(485124..486494) Mycobacterium ulcerans Agy99 4550679 YP_904648.1 CDS MUL_0474 NC_008611.1 486491 487315 R membrane protein; function unknown, domain homology to short-chain dehydrogenase/reductase family; short chain dehydrogenase complement(486491..487315) Mycobacterium ulcerans Agy99 4550680 YP_904649.1 CDS cyp123A3 NC_008611.1 487315 488535 R cytoplasmic protein; cytochrome P450s are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics; cytochrome P450 123A3 Cyp123A3 complement(487315..488535) Mycobacterium ulcerans Agy99 4550681 YP_904650.1 CDS MUL_0476 NC_008611.1 488532 488951 R membrane protein; hypothetical protein complement(488532..488951) Mycobacterium ulcerans Agy99 4550682 YP_904651.1 CDS aldA NC_008611.1 489411 490880 D cytoplasmic protein; oxidizes a variety of aldehydes [catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH]; NAD-dependent aldehyde dehydrogenase AldA 489411..490880 Mycobacterium ulcerans Agy99 4550683 YP_904652.1 CDS MUL_0478 NC_008611.1 490891 491643 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; short chain dehydrogenase 490891..491643 Mycobacterium ulcerans Agy99 4550684 YP_904653.1 CDS MUL_0480 NC_008611.1 492554 492991 D cytoplasmic protein; involved in aromatic hydrocarbons catabolism. thought to be involved in the catabolism of protocatechuate to succinate-and acetyl-CoA in the beta- ketoadipate pathway (at the third step) [catalytic activity: 2-carboxy-5-oxo-2,5-dihydrofuran-2-acetate = 5- oxo-4,5-dihydrofuran-2-acetate + CO(2)]; 4-carboxymuconolactone decarboxylase 492554..492991 Mycobacterium ulcerans Agy99 4550686 YP_904654.1 CDS purD NC_008611.1 493030 494298 D catalyzes the formation of N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-D-ribosylamine and glycine in purine biosynthesis; phosphoribosylamine--glycine ligase 493030..494298 Mycobacterium ulcerans Agy99 4550687 YP_904655.1 CDS pknF_2 NC_008611.1 494338 495840 R membrane protein; involved in signal transduction (via phosphorylation) [catalytic activity: ATP + a protein = ADP + a phosphoprotein]; serine/threonine-protein kinase PknF_2 complement(494338..495840) Mycobacterium ulcerans Agy99 4550688 YP_904656.1 CDS MUL_0483 NC_008611.1 496226 497140 D cytoplasmic protein; domain: UspA, universal stress protein UspA and related nucleotide-binding proteins [signal transduction mechanisms]; hypothetical protein 496226..497140 Mycobacterium ulcerans Agy99 4550689 YP_904657.1 CDS moeY NC_008611.1 497169 499463 R membrane protein; involved in biosynthesis of a demolybdo cofactor (molybdopterin), necessary for molybdoenzymes. plays a role in the activation of the small subunit of the molybdopterin converting factor (MoaD); hypothetical protein complement(497169..499463) Mycobacterium ulcerans Agy99 4550690 YP_904658.1 CDS MUL_0485 NC_008611.1 499537 500583 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 499537..500583 Mycobacterium ulcerans Agy99 4550691 YP_904659.1 CDS ggtA NC_008611.1 501119 502528 R cytoplasmic protein; besides the cephalosporin acylase I activity which converts GL-7ACA into 7-Aca; this enzyme displays some gamma glutamyltranspeptidase activity: Ggt plays a key role in the gamma-glutamyl cycle, a pathway for the synthesis and degradation of glutathione. [catalytic activity 1: 7-beta-(4-carboxybutanamido)-cephalosporanic acid + H2O = 7-aminocephalosporanic acid + glutaric acid] [catalytic activity 2: (5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl-amino acid]; bifunctional acylase, GgtA complement(501119..502528) Mycobacterium ulcerans Agy99 4550692 YP_904660.1 CDS MUL_0487 NC_008611.1 502580 503518 R membrane protein; function unknown, contains esterase domain; hypothetical protein complement(502580..503518) Mycobacterium ulcerans Agy99 4551981 YP_904661.1 CDS MUL_0488 NC_008611.1 503553 504122 D membrane protein; contains transcriptional regulatory domain; hypothetical protein 503553..504122 Mycobacterium ulcerans Agy99 4551982 YP_904662.1 CDS MUL_0489 NC_008611.1 504163 504924 R cytoplasmic protein; hypothetical protein complement(504163..504924) Mycobacterium ulcerans Agy99 4551983 YP_904663.1 CDS purB NC_008611.1 505043 506470 D Catalyzes two discrete reactions in the de novo synthesis of purines: the cleavage of adenylosuccinate and succinylaminoimidazole carboxamide ribotide; adenylosuccinate lyase 505043..506470 Mycobacterium ulcerans Agy99 4551984 YP_904664.1 CDS cyp126A3 NC_008611.1 506467 507723 D cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.; cytochrome P450 126A3 Cyp126A3 506467..507723 Mycobacterium ulcerans Agy99 4551985 YP_904665.1 CDS MUL_0492 NC_008611.1 508010 509218 D membrane protein; PPE family protein 508010..509218 Mycobacterium ulcerans Agy99 4551986 YP_904666.1 CDS MUL_0493 NC_008611.1 509422 510711 D cytoplasmic protein; possibly involed in transcription regulation; DNA- binding.; putative regulatory protein 509422..510711 Mycobacterium ulcerans Agy99 4551987 YP_904667.1 CDS MUL_0494 NC_008611.1 510843 512366 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (LC-MS/MS); cytoplasmic protein; interconversion aldehyde and acid [catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH]; aldehyde dehydrogenase 510843..512366 Mycobacterium ulcerans Agy99 4551988 YP_904668.1 CDS adhX NC_008611.1 512418 513479 D cytoplasmic protein; Zn-dependent alcohol dehydrogenase, AdhX 512418..513479 Mycobacterium ulcerans Agy99 4551989 YP_904669.1 CDS MUL_0496 NC_008611.1 513470 514090 R membrane protein; transmembrane protein complement(513470..514090) Mycobacterium ulcerans Agy99 4551990 YP_904670.1 CDS purC NC_008611.1 514155 515048 D catalyzes the formation of (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate and L-aspartate in purine biosynthesis; SAICAR synthase; phosphoribosylaminoimidazole-succinocarboxamide synthase 514155..515048 Mycobacterium ulcerans Agy99 4551991 YP_904671.1 CDS ptrB NC_008611.1 515027 517162 D cytoplasmic protein; cleaves peptide bonds on the C-terminal side of LysYL and argininyl residues [catalytic activity: hydrolysis of arg-|-xaa and lys-|-xaa bonds in oligopeptides, even when P1' residue is proline]; protease II (oligopeptidase B), PtrB 515027..517162 Mycobacterium ulcerans Agy99 4551992 YP_904672.1 CDS MUL_0499 NC_008611.1 517121 517570 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(517121..517570) Mycobacterium ulcerans Agy99 4551993 YP_904673.1 CDS MUL_0500 NC_008611.1 517665 518381 D cytoplasmic protein; TetR-family; transcriptional regulator 517665..518381 Mycobacterium ulcerans Agy99 4551994 YP_904674.1 CDS MUL_0501 NC_008611.1 518175 519836 R membrane protein; translocase that confers resistance to substances of high hydrophobicity. involved in transport of multidrug across the membrane (export): multidrug resistance by an export mechanism. responsible for the translocation of the substrate across the membrane.; integral membrane drug efflux protein complement(518175..519836) Mycobacterium ulcerans Agy99 4551995 YP_904675.1 CDS MUL_0502 NC_008611.1 520005 520709 D cytoplasmic protein; hypothetical protein 520005..520709 Mycobacterium ulcerans Agy99 4551996 YP_904676.1 CDS MUL_0503 NC_008611.1 520667 521713 R membrane protein; hypothetical protein complement(520667..521713) Mycobacterium ulcerans Agy99 4551997 YP_904677.1 CDS MUL_0504 NC_008611.1 521765 521959 R hypothetical protein complement(521765..521959) Mycobacterium ulcerans Agy99 4551998 YP_904678.1 CDS MUL_0505 NC_008611.1 521934 522296 R secreted protein; hypothetical protein complement(521934..522296) Mycobacterium ulcerans Agy99 4551999 YP_904679.1 CDS MUL_0506 NC_008611.1 522629 522979 D cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 522629..522979 Mycobacterium ulcerans Agy99 4550747 YP_904680.1 CDS MUL_0507 NC_008611.1 523049 523675 D cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 523049..523675 Mycobacterium ulcerans Agy99 4550748 YP_904681.1 CDS MUL_0508 NC_008611.1 523885 524253 D cytoplasmic protein; hypothetical protein 523885..524253 Mycobacterium ulcerans Agy99 4550749 YP_904682.1 CDS MUL_0509 NC_008611.1 524368 524871 D cytoplasmic protein; excisionase 524368..524871 Mycobacterium ulcerans Agy99 4550750 YP_904683.1 CDS MUL_0510 NC_008611.1 524902 525072 D cytoplasmic protein; hypothetical protein 524902..525072 Mycobacterium ulcerans Agy99 4550751 YP_904684.1 CDS MUL_0511 NC_008611.1 525269 525502 D cytoplasmic protein; hypothetical protein 525269..525502 Mycobacterium ulcerans Agy99 4550752 YP_904685.1 CDS MUL_0512 NC_008611.1 525804 527240 D cytoplasmic protein; hypothetical protein 525804..527240 Mycobacterium ulcerans Agy99 4550753 YP_904686.1 CDS MUL_0513 NC_008611.1 527247 528020 R cytoplasmic protein; hypothetical protein complement(527247..528020) Mycobacterium ulcerans Agy99 4550754 YP_904687.1 CDS MUL_0515 NC_008611.1 528829 530424 D cytoplasmic protein; hypothetical protein 528829..530424 Mycobacterium ulcerans Agy99 4550756 YP_904688.1 CDS MUL_0516 NC_008611.1 530613 531470 D cytoplasmic protein; hypothetical protein 530613..531470 Mycobacterium ulcerans Agy99 4550757 YP_904689.1 CDS MUL_0517 NC_008611.1 531483 531635 D cytoplasmic protein; hypothetical protein 531483..531635 Mycobacterium ulcerans Agy99 4550758 YP_904690.1 CDS MUL_0518 NC_008611.1 531637 532416 D membrane protein; frameshift mutant?; hypothetical protein 531637..532416 Mycobacterium ulcerans Agy99 4550759 YP_904691.1 CDS MUL_0520 NC_008611.1 533642 534985 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 533642..534985 Mycobacterium ulcerans Agy99 4550761 YP_904692.1 CDS MUL_0521 NC_008611.1 535354 536400 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 535354..536400 Mycobacterium ulcerans Agy99 4552236 YP_904693.1 CDS MUL_0523 NC_008611.1 537317 537520 D cytoplasmic protein; hypothetical protein 537317..537520 Mycobacterium ulcerans Agy99 4552238 YP_904694.1 CDS MUL_0524 NC_008611.1 537534 537968 R cytoplasmic protein; may be an ADP-ribose pyrophosphatase - involved DNA replication, recombination, and repair; hypothetical protein complement(537534..537968) Mycobacterium ulcerans Agy99 4552239 YP_904695.1 CDS MUL_0525 NC_008611.1 538041 538835 D GntR-family transcriptional regulator; Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; transcriptional regulatory protein 538041..538835 Mycobacterium ulcerans Agy99 4552240 YP_904696.1 CDS MUL_0526 NC_008611.1 538935 539357 D cytoplasmic protein; hypothetical protein 538935..539357 Mycobacterium ulcerans Agy99 4552241 YP_904697.1 CDS MUL_0527 NC_008611.1 539414 540676 D membrane protein; contains FtsK domain: FtsK has extensive sequence similarity to wide variety of proteins from prokaryotes and plasmids, termed the FtsK/SpoIIIE family. this domain contains a putative ATP binding P-loop motif. it is found in the FtsK cell division protein from E. coli and the stage III sporulation protein E SpoIIIE, which has roles in regulation of prespore specific gene expression in B. subtilis. a mutation in FtsK causes a temperature sensitive block in cell division and it is involved in peptidoglycan synthesis or modification. the SpoIIIE protein is implicated in intercellular chromosomal DNA transfer.; hypothetical protein 539414..540676 Mycobacterium ulcerans Agy99 4552242 YP_904698.1 CDS MUL_0528 NC_008611.1 540685 540948 D cytoplasmic protein; hypothetical protein 540685..540948 Mycobacterium ulcerans Agy99 4552243 YP_904699.1 CDS MUL_0529 NC_008611.1 540948 542117 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS; cytoplasmic protein; part of a prophage not present in M. marinum; prophage integrase 540948..542117 Mycobacterium ulcerans Agy99 4552244 YP_904700.1 CDS MUL_0535 NC_008611.1 543034 544080 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(543034..544080) Mycobacterium ulcerans Agy99 4549991 YP_904701.1 CDS echA8_3 NC_008611.1 544542 545348 R Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase complement(544542..545348) Mycobacterium ulcerans Agy99 4549992 YP_904702.1 CDS MUL_0538 NC_008611.1 546180 547226 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 546180..547226 Mycobacterium ulcerans Agy99 4549994 YP_904703.1 CDS amiC_2 NC_008611.1 548714 550207 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; hydrolyzes a monocarboxylic acid amide and generates a monocarboxylate [catalytic activity: a monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)]; amidase 548714..550207 Mycobacterium ulcerans Agy99 4549995 YP_904704.1 CDS MUL_0542 NC_008611.1 552061 553314 D membrane protein; function unknown, facilitates transport of small solutes in or out of the cell.; multidrug resistance integral membrane efflux protein 552061..553314 Mycobacterium ulcerans Agy99 4549997 YP_904705.1 CDS MUL_0544 NC_008611.1 553832 554593 D membrane protein; dehydrogenase 553832..554593 Mycobacterium ulcerans Agy99 4549999 YP_904706.1 CDS MUL_0545 NC_008611.1 554604 555272 D cytoplasmic protein; hydrolase 554604..555272 Mycobacterium ulcerans Agy99 4550000 YP_904707.1 CDS MUL_0546 NC_008611.1 555279 556121 D membrane protein; domain homology to NagC, transcriptional regulator/sugar kinase [transcription / carbohydrate transport and metabolism]; glucose kinase 555279..556121 Mycobacterium ulcerans Agy99 4550001 YP_904708.1 CDS MUL_0548 NC_008611.1 557383 558168 D membrane protein; epimerase 557383..558168 Mycobacterium ulcerans Agy99 4550003 YP_904709.1 CDS MUL_0549 NC_008611.1 558273 559166 D cytoplasmic protein; hypothetical protein 558273..559166 Mycobacterium ulcerans Agy99 4550004 YP_904710.1 CDS MUL_5123 NC_008611.1 559251 559460 R cytoplasmic protein; hypothetical protein complement(559251..559460) Mycobacterium ulcerans Agy99 4550005 YP_904711.1 CDS MUL_0550 NC_008611.1 559999 561045 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 559999..561045 Mycobacterium ulcerans Agy99 4550693 YP_904712.1 CDS MUL_0551 NC_008611.1 561042 561290 R cytoplasmic protein; hypothetical protein complement(561042..561290) Mycobacterium ulcerans Agy99 4550694 YP_904713.1 CDS MUL_0552 NC_008611.1 561291 561713 R cytoplasmic protein; hypothetical protein complement(561291..561713) Mycobacterium ulcerans Agy99 4550695 YP_904714.1 CDS MUL_0554 NC_008611.1 562014 562793 R membrane protein; involved in transcriptional mechanism.; transcriptional regulatory protein complement(562014..562793) Mycobacterium ulcerans Agy99 4550697 YP_904715.1 CDS MUL_0556 NC_008611.1 564418 564798 D cytoplasmic protein; hypothetical protein 564418..564798 Mycobacterium ulcerans Agy99 4550699 YP_904716.1 CDS MUL_0559 NC_008611.1 565709 566377 D secreted protein; maybe exported; hypothetical protein 565709..566377 Mycobacterium ulcerans Agy99 4550701 YP_904717.1 CDS dcd NC_008611.1 566423 566995 D Catalyzes the formation of dUTP from dCTP in thymidylate biosynthesis; deoxycytidine triphosphate deaminase 566423..566995 Mycobacterium ulcerans Agy99 4550702 YP_904718.1 CDS MUL_0562 NC_008611.1 568350 568718 R cytoplasmic protein; hypothetical protein complement(568350..568718) Mycobacterium ulcerans Agy99 4550704 YP_904719.1 CDS MUL_0566 NC_008611.1 571803 572558 D cytoplasmic protein; hypothetical protein 571803..572558 Mycobacterium ulcerans Agy99 4550707 YP_904720.1 CDS MUL_0567 NC_008611.1 572585 572968 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein 572585..572968 Mycobacterium ulcerans Agy99 4550708 YP_904721.1 CDS rmlA NC_008611.1 572998 573864 D cytoplasmic protein; dTDP-L-rhamnose biosynthesis within the O antigen biosynthesis pathway of lipopolysaccharide biosynthesis [catalytic activity: dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-glucose]; alpha-D-glucose-1-phosphate thymidylyl- transferase, RmlA 572998..573864 Mycobacterium ulcerans Agy99 4552727 YP_904722.1 CDS MUL_0569 NC_008611.1 573898 574929 R membrane protein; function unknown. seems to influence both cell surface interactions among mycobacteria and the interactions of the bacteria with macrophages.; PE PGRS family protein complement(573898..574929) Mycobacterium ulcerans Agy99 4552728 YP_904723.1 CDS MUL_0570 NC_008611.1 575111 576157 R membrane protein; PE PGRS family protein complement(575111..576157) Mycobacterium ulcerans Agy99 4552729 YP_904724.1 CDS MUL_0571 NC_008611.1 576289 577182 R cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 complement(576289..577182) Mycobacterium ulcerans Agy99 4552730 YP_904725.1 CDS MUL_0572 NC_008611.1 577925 580594 D membrane protein; function unknown, member of the mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins; PE-PGRS family protein 577925..580594 Mycobacterium ulcerans Agy99 4552731 YP_904726.1 CDS aspC NC_008611.1 580688 581977 R broad specificity; family IV; in Corynebacterium glutamicum this protein can use glutamate, 2-aminobutyrate, and aspartate as amino donors and pyruvate as the acceptor; aminotransferase AlaT complement(580688..581977) Mycobacterium ulcerans Agy99 4552732 YP_904727.1 CDS MUL_0574 NC_008611.1 582008 584956 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; iron-sulfur-binding reductase complement(582008..584956) Mycobacterium ulcerans Agy99 4552733 YP_904728.1 CDS MUL_0575 NC_008611.1 585218 586561 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(585218..586561) Mycobacterium ulcerans Agy99 4552734 YP_904729.1 CDS MUL_0576 NC_008611.1 587077 587970 D cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 587077..587970 Mycobacterium ulcerans Agy99 4552735 YP_904730.1 CDS MUL_0578 NC_008611.1 590363 590902 D cytoplasmic protein; hypothetical protein 590363..590902 Mycobacterium ulcerans Agy99 4552737 YP_904731.1 CDS iniB NC_008611.1 591184 594051 D membrane protein; function unknown function but ortholog in M.tuberculosis induced by isoniazid and ethionamide.; membrane protein, IniB 591184..594051 Mycobacterium ulcerans Agy99 4552738 YP_904732.1 CDS iniA NC_008611.1 594152 595993 D membrane protein; in M. tuberculosis H37Rv this gene is induced by isoniazid (INH) or ethionamide treatment) (see Wilson et al., 1999); hypothetical protein 594152..595993 Mycobacterium ulcerans Agy99 4552739 YP_904733.1 CDS iniC NC_008611.1 595990 597471 D membrane protein; in M. tuberculosis H37Rv IniC is an isoniazid- inducible gene; protein IniC 595990..597471 Mycobacterium ulcerans Agy99 4552740 YP_904734.1 CDS MUL_0582 NC_008611.1 597476 599296 R membrane protein; hypothetical protein complement(597476..599296) Mycobacterium ulcerans Agy99 4552741 YP_904735.1 CDS MUL_0583 NC_008611.1 599379 599903 R membrane protein; hypothetical protein complement(599379..599903) Mycobacterium ulcerans Agy99 4553498 YP_904736.1 CDS MUL_0584 NC_008611.1 600011 600592 R membrane protein; hypothetical protein complement(600011..600592) Mycobacterium ulcerans Agy99 4553499 YP_904737.1 CDS lpqJ NC_008611.1 600629 601192 R membrane protein; lipoprotein LpqJ complement(600629..601192) Mycobacterium ulcerans Agy99 4553500 YP_904738.1 CDS coxM_2 NC_008611.1 601753 602640 R cytoplasmic protein; aerobic-type carbon monoxide dehydrogenase subunit CoxM_2 complement(601753..602640) Mycobacterium ulcerans Agy99 4553502 YP_904739.1 CDS coxL_2 NC_008611.1 602637 604967 R cytoplasmic protein; aerobic-type carbon monoxide dehydrogenase subunit CoxL_2 complement(602637..604967) Mycobacterium ulcerans Agy99 4553503 YP_904740.1 CDS MUL_0589 NC_008611.1 605118 605648 D membrane protein; hypothetical protein 605118..605648 Mycobacterium ulcerans Agy99 4553504 YP_904741.1 CDS dnaK NC_008611.1 608083 609951 D heat shock protein 70; assists in folding of nascent polypeptide chains; refolding of misfolded proteins; utilizes ATPase activity to help fold; co-chaperones are DnaJ and GrpE; multiple copies in some bacteria; molecular chaperone DnaK 608083..609951 Mycobacterium ulcerans Agy99 4553507 YP_904742.1 CDS grpE NC_008611.1 609948 610601 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the extracellular matrix by proteomics.; cytoplasmic protein; stimulates, jointly with DnaJ, the ATPase activity of DnaK. helps to release ADP from DnaK thus allowing DnaK to recycle more efficiently. seems to be regulated negatively by HspR.; GrpE protein (Hsp-70 cofactor) 609948..610601 Mycobacterium ulcerans Agy99 4553508 YP_904743.1 CDS dnaJ NC_008611.1 610659 611849 D chaperone Hsp40; co-chaperone with DnaK; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion; chaperone protein DnaJ 610659..611849 Mycobacterium ulcerans Agy99 4553509 YP_904744.1 CDS hspR NC_008611.1 611850 612332 D Extended C-term caused by point mutation removing stop codon. This CDS now overlap the C-term of the d/s CDS.; cytoplasmic protein; involved in transcriptional regulation (repression) of heat shock proteins e.g. DnaK, GrpE, DnaJ. binds to three inverted repeats (IR1-IR3) in the promoter region of the DnaK operon. induction: by heat shock.; heat shock protein transcriptional repressor HspR 611850..612332 Mycobacterium ulcerans Agy99 4553510 YP_904745.1 CDS MUL_0598 NC_008611.1 616018 617352 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 616018..617352 Mycobacterium ulcerans Agy99 4553512 YP_904746.1 CDS MUL_0601 NC_008611.1 619158 620489 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(619158..620489) Mycobacterium ulcerans Agy99 4550762 YP_904747.1 CDS cyp185A4 NC_008611.1 622440 623855 R cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. some have been shown to bind tightly to a range of azole-based antifungal drugs (E.G. miconazole, clotrimazole); cytochrome P450 185A4 Cyp185A4 complement(622440..623855) Mycobacterium ulcerans Agy99 4550763 YP_904748.1 CDS MUL_0605 NC_008611.1 624817 625266 R cytoplasmic protein; hypothetical protein complement(624817..625266) Mycobacterium ulcerans Agy99 4550764 YP_904749.1 CDS MUL_0606 NC_008611.1 625492 626829 D membrane protein; membrane acyltransferase 625492..626829 Mycobacterium ulcerans Agy99 4550765 YP_904750.1 CDS MUL_0607 NC_008611.1 626914 627666 D secreted protein; function unknown, domain identity to secreted hydrolases of the SgnH_hydrolase subfamily; hypothetical protein 626914..627666 Mycobacterium ulcerans Agy99 4550766 YP_904751.1 CDS MUL_0608 NC_008611.1 627736 627852 R membrane protein; hypothetical protein complement(627736..627852) Mycobacterium ulcerans Agy99 4550767 YP_904752.1 CDS MUL_0609 NC_008611.1 627964 630009 R membrane protein; transmembrane acyltransferase complement(627964..630009) Mycobacterium ulcerans Agy99 4550768 YP_904753.1 CDS MUL_0610 NC_008611.1 630176 631084 R membrane protein; has homology to a putative esterase domain; hypothetical protein complement(630176..631084) Mycobacterium ulcerans Agy99 4550769 YP_904754.1 CDS MUL_0611 NC_008611.1 631231 632223 D cytoplasmic protein; some similarity to universal stress protein UspA.; hypothetical protein 631231..632223 Mycobacterium ulcerans Agy99 4550770 YP_904755.1 CDS ctpF NC_008611.1 632275 634992 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; metal cation-transporting ATPase; possibly catalyzes the transport of a undeterminated metal cation with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation(out); metal cation-transporting p-type ATPase F, CtpF 632275..634992 Mycobacterium ulcerans Agy99 4550771 YP_904756.1 CDS MUL_0614 NC_008611.1 636161 637207 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(636161..637207) Mycobacterium ulcerans Agy99 4550773 YP_904757.1 CDS apbA NC_008611.1 639830 640747 R membrane protein; ketopantoate reductase PanE/ApbA. this is a family of 2-dehydropantoate 2-reductases also known as ketopantoate reductases. the reaction catalysed by this enzyme is: (R)-pantoate + NADP(+) <=> 2-dehydropantoate + NADPH. AbpA catalyses the NADPH reduction of ketopantoic acid to pantoic acid in the alternative pyrimidine biosynthetic (APB) pathway. ApbA and pane are allelic. ApbA, the ketopantoate reductase enzyme is required for the synthesis of thiamine via the APB biosynthetic pathway.; ketopantoate reductase, ApbA complement(639830..640747) Mycobacterium ulcerans Agy99 4550775 YP_904758.1 CDS MUL_0619 NC_008611.1 642133 642480 D cytoplasmic protein; hypothetical protein 642133..642480 Mycobacterium ulcerans Agy99 4550777 YP_904759.1 CDS MUL_0620 NC_008611.1 642477 642776 R cytoplasmic protein; hypothetical protein complement(642477..642776) Mycobacterium ulcerans Agy99 4550778 YP_904760.1 CDS hemL NC_008611.1 645805 647136 D Converts (S)-4-amino-5-oxopentanoate to 5-aminolevulinate during the porphyrin biosynthesis pathway; glutamate-1-semialdehyde aminotransferase 645805..647136 Mycobacterium ulcerans Agy99 4551620 YP_904761.1 CDS MUL_0624 NC_008611.1 647136 647744 D cytoplasmic protein; contains fructose-2,6-bisphosphatase domain [carbohydrate transport and metabolism]; hypothetical protein 647136..647744 Mycobacterium ulcerans Agy99 4551621 YP_904762.1 CDS MUL_0625 NC_008611.1 647741 648358 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; thioredoxin protein 647741..648358 Mycobacterium ulcerans Agy99 4551622 YP_904763.1 CDS ccsA NC_008611.1 648355 649146 D membrane protein; required during cytochrome biogenesis at the step of HemE attachment.; cytochrome C-type biogenesis protein, CcsA 648355..649146 Mycobacterium ulcerans Agy99 4551623 YP_904764.1 CDS MUL_0627 NC_008611.1 649143 650822 D membrane protein; required for cytochrome C biosynthesis; ResB-family protein 649143..650822 Mycobacterium ulcerans Agy99 4551624 YP_904765.1 CDS ccsB NC_008611.1 650819 651805 D membrane protein; required during cytochrome biogenesis at the step of HemE attachment.; cytochrome C-type biogenesis protein, CcsB 650819..651805 Mycobacterium ulcerans Agy99 4551625 YP_904766.1 CDS MUL_0630 NC_008611.1 653170 653322 R membrane protein; hypothetical protein complement(653170..653322) Mycobacterium ulcerans Agy99 4551627 YP_904767.1 CDS MUL_0631 NC_008611.1 653369 653737 D membrane protein; hypothetical protein 653369..653737 Mycobacterium ulcerans Agy99 4551628 YP_904768.1 CDS fabH NC_008611.1 653745 654752 R FabH; beta-ketoacyl-acyl carrier protein synthase III; catalyzes the condensation of acetyl-CoA with malonyl-ACP to initiate cycles of fatty acid elongation; differs from 3-oxoacyl-(acyl carrier protein) synthase I and II in that it utilizes CoA thioesters as primers rather than acyl-ACPs; 3-oxoacyl-ACP synthase complement(653745..654752) Mycobacterium ulcerans Agy99 4551629 YP_904769.1 CDS menA NC_008611.1 654851 655777 R catalyzes the formation of dimethylmenaquinone from 1,4-dihydroxy-2-naphthoate and octaprenyl diphosphate; 1,4-dihydroxy-2-naphthoate octaprenyltransferase complement(654851..655777) Mycobacterium ulcerans Agy99 4551630 YP_904770.1 CDS pnp NC_008611.1 655767 656561 D Catalyzes the reversible phosphorolysis of 5'-deoxy-5'- methylthioadenosine (MTA) to adenine and 5-methylthio-D-ribose-1- phosphate; 5'-methylthioadenosine phosphorylase 655767..656561 Mycobacterium ulcerans Agy99 4551631 YP_904771.1 CDS galE3 NC_008611.1 656558 657598 D cytoplasmic protein; involved in galactose metabolism [catalytic activity: UDP-glucose = UDP-galactose]; UDP-glucose 4-epimerase 656558..657598 Mycobacterium ulcerans Agy99 4551632 YP_904772.1 CDS MUL_0636 NC_008611.1 657606 659228 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(657606..659228) Mycobacterium ulcerans Agy99 4551633 YP_904773.1 CDS MUL_0637 NC_008611.1 659546 661390 D membrane protein; hypothetical protein 659546..661390 Mycobacterium ulcerans Agy99 4551634 YP_904774.1 CDS MUL_0638 NC_008611.1 661420 662076 D cytoplasmic protein; substrate (sugar) unknown [catalytic activity: NDP- sugar + dolichyl phosphate = NDP + dolichyl sugar phosphate]; glycosyl transferase - dolichol-P-sugar synthase 661420..662076 Mycobacterium ulcerans Agy99 4551635 YP_904775.1 CDS MUL_0639 NC_008611.1 662073 662735 D cytoplasmic protein; hypothetical protein 662073..662735 Mycobacterium ulcerans Agy99 4551636 YP_904776.1 CDS MUL_0640 NC_008611.1 662767 664119 R membrane protein; hypothetical protein complement(662767..664119) Mycobacterium ulcerans Agy99 4551637 YP_904777.1 CDS menE NC_008611.1 664131 665240 R cytoplasmic protein; involved in menaquinone biosynthesis. O- succinylbenzoic acid (OSB) to O-succinylbenzoyl-CoA (OSB- CoA) [catalytic activity: ATP + O-succinylbenzoate + CoA = AMP + diphosphate + O-succinylbenzoyl-CoA]; O-succinylbenzoic acid--CoA ligase complement(664131..665240) Mycobacterium ulcerans Agy99 4551805 YP_904778.1 CDS MUL_0642 NC_008611.1 665285 665581 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); cytoplasmic protein; hypothetical protein complement(665285..665581) Mycobacterium ulcerans Agy99 4551806 YP_904779.1 CDS MUL_0643 NC_008611.1 665641 665925 R membrane protein; hypothetical protein complement(665641..665925) Mycobacterium ulcerans Agy99 4551807 YP_904780.1 CDS MUL_0645 NC_008611.1 667315 667722 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(667315..667722) Mycobacterium ulcerans Agy99 4551809 YP_904781.1 CDS MUL_0646 NC_008611.1 667736 668365 R cytoplasmic protein; hypothetical protein complement(667736..668365) Mycobacterium ulcerans Agy99 4551810 YP_904782.1 CDS MUL_0647 NC_008611.1 668384 669271 R Detected in the membrane fraction by proteomics. Also detected in the cytoplasmic fraction by 2D-LC-MS/MS.; cytoplasmic protein; short chain dehydrogenase complement(668384..669271) Mycobacterium ulcerans Agy99 4551811 YP_904783.1 CDS menB NC_008611.1 669315 670217 R catalyzes the formation of 1,4-dihydroxy-2-naphthoate from O-succinylbenzoyl-CoA; naphthoate synthase complement(669315..670217) Mycobacterium ulcerans Agy99 4551812 YP_904784.1 CDS MUL_0649 NC_008611.1 670214 670549 R cytoplasmic protein; hypothetical protein complement(670214..670549) Mycobacterium ulcerans Agy99 4551813 YP_904785.1 CDS fadD8 NC_008611.1 671026 672627 R activates fatty acids by binding to coenzyme A; acyl-CoA synthetase complement(671026..672627) Mycobacterium ulcerans Agy99 4551814 YP_904786.1 CDS MUL_0651 NC_008611.1 672716 674320 D membrane protein; has significant domain identity to predicted metal- dependent hydrolases; hypothetical protein 672716..674320 Mycobacterium ulcerans Agy99 4551815 YP_904787.1 CDS menC NC_008611.1 674317 675300 D catalyzes the dehydration of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid to form O-succinylbenzoate; O-succinylbenzoate synthase 674317..675300 Mycobacterium ulcerans Agy99 4551816 YP_904788.1 CDS bpoC NC_008611.1 675293 676081 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; non-haem peroxidase; bromoperoxidase BpoC 675293..676081 Mycobacterium ulcerans Agy99 4551817 YP_904789.1 CDS menD NC_008611.1 676145 677791 D SEPHCHC synthase; forms 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1- carboxylate from 2-oxoglutarate and isochorismate in menaquinone biosynthesis; 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase 676145..677791 Mycobacterium ulcerans Agy99 4551818 YP_904790.1 CDS MUL_0655 NC_008611.1 677788 678312 D membrane protein; hypothetical protein 677788..678312 Mycobacterium ulcerans Agy99 4551819 YP_904791.1 CDS pimB NC_008611.1 678388 679539 D cytoplasmic protein; involved in lipoarabinomannan (lam) biosynthesis.; mannosyltransferase, PimB 678388..679539 Mycobacterium ulcerans Agy99 4551820 YP_904792.1 CDS MUL_0657 NC_008611.1 679536 680138 R cytoplasmic protein; function unknown, mar family; transcriptional regulator complement(679536..680138) Mycobacterium ulcerans Agy99 4551821 YP_904793.1 CDS MUL_0658 NC_008611.1 680149 681342 D cytoplasmic protein; hypothetical protein 680149..681342 Mycobacterium ulcerans Agy99 4551822 YP_904794.1 CDS MUL_0659 NC_008611.1 681332 682639 D cytoplasmic protein; hypothetical protein 681332..682639 Mycobacterium ulcerans Agy99 4551823 YP_904795.1 CDS ubiE NC_008611.1 682668 683363 D Catalyzes the carbon methylation reaction in the biosynthesis of ubiquinone; ubiquinone/menaquinone biosynthesis methyltransferase 682668..683363 Mycobacterium ulcerans Agy99 4553382 YP_904796.1 CDS MUL_0661 NC_008611.1 683382 683717 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; hypothetical protein complement(683382..683717) Mycobacterium ulcerans Agy99 4553383 YP_904797.1 CDS MUL_0662 NC_008611.1 683707 684438 R cytoplasmic protein; methyltransferase complement(683707..684438) Mycobacterium ulcerans Agy99 4553384 YP_904798.1 CDS MUL_0663 NC_008611.1 684504 685742 R membrane protein; has significant domain conservation with dehydrogenases (flavoproteins) [energy production and conversion]; FAD-linked oxidoreductase complement(684504..685742) Mycobacterium ulcerans Agy99 4553385 YP_904799.1 CDS grcC1 NC_008611.1 685764 686771 D cytoplasmic protein; supplies polyprenyl diphosphate; polyprenyl diphosphate synthetase, GrcC1 685764..686771 Mycobacterium ulcerans Agy99 4553386 YP_904800.1 CDS hspX NC_008611.1 686862 687722 D putative metalloprotease; heat shock protein HtpX 686862..687722 Mycobacterium ulcerans Agy99 4553387 YP_904801.1 CDS sulP NC_008611.1 687743 689344 R membrane protein; generates CO(2) and H(2)O from H(2)CO(3), and possibly involved in transport of sulfate across the membrane.; transmembrane carbonic anhydrase, SulP complement(687743..689344) Mycobacterium ulcerans Agy99 4553388 YP_904802.1 CDS sulP_1 NC_008611.1 689637 691190 R membrane protein; frameshift; transmembrane carbonic anhydrase, SulP complement(689637..691190) Mycobacterium ulcerans Agy99 4553389 YP_904803.1 CDS gpdA1 NC_008611.1 691423 692448 R catalyzes the NAD(P)H-dependent reduction of glycerol 3-phosphate to glycerone phosphate; NAD(P)H-dependent glycerol-3-phosphate dehydrogenase complement(691423..692448) Mycobacterium ulcerans Agy99 4553390 YP_904804.1 CDS MUL_0668 NC_008611.1 692900 693595 D membrane protein; hydrolyzes peptides and/or proteins. cleaves preferentially after serine residues; serine protease 692900..693595 Mycobacterium ulcerans Agy99 4553391 YP_904805.1 CDS rimL NC_008611.1 697302 697760 R cytoplasmic protein; domain identity to N-acetylases of ribosomal proteins; acetyltransferase, RimL complement(697302..697760) Mycobacterium ulcerans Agy99 4553393 YP_904806.1 CDS MUL_0671 NC_008611.1 697968 698459 R putative nucleotide binding property based on structural studies of Haemophilus influenzae crystallized protein in PDB Accession Number 1IN0 and NMR studies of Escherichia coli YajQ; the YajQ protein from Pseudomonas synringae appears to play a role in activation of bateriophage phi6 segment L transcription; putative nucleotide-binding protein complement(697968..698459) Mycobacterium ulcerans Agy99 4553394 YP_904807.1 CDS MUL_0673 NC_008611.1 698652 700913 R membrane protein; ABC-type transport system, involved in lipoprotein release, permease component; ABC transporter complement(698652..700913) Mycobacterium ulcerans Agy99 4553396 YP_904808.1 CDS MUL_0674 NC_008611.1 701006 701740 R membrane protein; ABC transporter ATP-binding protein complement(701006..701740) Mycobacterium ulcerans Agy99 4553397 YP_904809.1 CDS MUL_0675 NC_008611.1 701737 702342 R cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulator complement(701737..702342) Mycobacterium ulcerans Agy99 4553398 YP_904810.1 CDS MUL_0676 NC_008611.1 702465 703277 R cytoplasmic protein; carveol-like dehydrogenase complement(702465..703277) Mycobacterium ulcerans Agy99 4553399 YP_904811.1 CDS nhaP NC_008611.1 703399 704619 R membrane protein; NhaP-type Na+/H+ and K+/H+ antiporter: inorganic ion transport and metabolism] antiporters are ubiquitous membrane proteins that are involved in homeostasis of H(+) and na(+) throughout the biological kingdom.; ion antiporter, NhaP complement(703399..704619) Mycobacterium ulcerans Agy99 4553400 YP_904812.1 CDS MUL_0678 NC_008611.1 704778 705824 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(704778..705824) Mycobacterium ulcerans Agy99 4554462 YP_904813.1 CDS MUL_0679 NC_008611.1 706434 706631 D hypothetical protein 706434..706631 Mycobacterium ulcerans Agy99 4554463 YP_904814.1 CDS MUL_0681 NC_008611.1 707984 708607 D cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator 707984..708607 Mycobacterium ulcerans Agy99 4554465 YP_904815.1 CDS MUL_0685 NC_008611.1 710901 711080 D membrane protein; hypothetical protein 710901..711080 Mycobacterium ulcerans Agy99 4554469 YP_904816.1 CDS MUL_0686 NC_008611.1 711204 712226 D cytoplasmic protein; methyltransferase/methylase 711204..712226 Mycobacterium ulcerans Agy99 4554470 YP_904817.1 CDS MUL_0687 NC_008611.1 712367 713098 D cytoplasmic protein; adenylate cyclase 712367..713098 Mycobacterium ulcerans Agy99 4554471 YP_904818.1 CDS gatA_1 NC_008611.1 715636 717156 D cytoplasmic protein; component of the translational apparatus. furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. the reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-glu-tRNA(Gln) [catalytic activity: ATP + L-glutamyl-tRNA(Gln) + L- glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L- glutamate]; peptide amidase, GatA 715636..717156 Mycobacterium ulcerans Agy99 4554473 YP_904819.1 CDS MUL_0690 NC_008611.1 717353 717700 R cytoplasmic protein; oxidoreductase complement(717353..717700) Mycobacterium ulcerans Agy99 4554474 YP_904820.1 CDS galT NC_008611.1 717732 718889 D membrane protein; involved in galactose metabolism (Leloir pathway) [catalytic activity: UTP + alpha-D-galactose 1-phosphate = diphosphate + UDP-galactose]; galactose-1-phosphate uridylyltransferase 717732..718889 Mycobacterium ulcerans Agy99 4554475 YP_904821.1 CDS galK NC_008611.1 718886 719986 D catalyzes the formation of alpha-D-galactose 1-phosphate from D-galactose in galactose metabolism; galactokinase 718886..719986 Mycobacterium ulcerans Agy99 4554476 YP_904822.1 CDS MUL_0693 NC_008611.1 719983 721089 D membrane protein; ArsR family; transcriptional regulator 719983..721089 Mycobacterium ulcerans Agy99 4554477 YP_904823.1 CDS MUL_0695 NC_008611.1 724517 725329 D cytoplasmic protein; hypothetical protein 724517..725329 Mycobacterium ulcerans Agy99 4553823 YP_904824.1 CDS MUL_0698 NC_008611.1 728621 730093 R membrane protein; contains hydrolase domain; hypothetical protein complement(728621..730093) Mycobacterium ulcerans Agy99 4553826 YP_904825.1 CDS MUL_0699 NC_008611.1 730021 730602 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(730021..730602) Mycobacterium ulcerans Agy99 4553827 YP_904826.1 CDS lppF NC_008611.1 730716 732011 R membrane protein; lipoprotein LppF complement(730716..732011) Mycobacterium ulcerans Agy99 4553828 YP_904827.1 CDS lpqN NC_008611.1 732215 732895 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; lipoprotein LpqN complement(732215..732895) Mycobacterium ulcerans Agy99 4553829 YP_904828.1 CDS MUL_0702 NC_008611.1 732899 733135 D hypothetical protein 732899..733135 Mycobacterium ulcerans Agy99 4553830 YP_904829.1 CDS MUL_0704 NC_008611.1 735808 738183 R Also detected in the cytoplamic fraction by LCMSMS; membrane protein; integral membrane protein complement(735808..738183) Mycobacterium ulcerans Agy99 4553832 YP_904830.1 CDS MUL_0705 NC_008611.1 738681 739943 R cytoplasmic protein; function unknown, imidazolonepropionase is an amidohydrolase; imidazolonepropionase complement(738681..739943) Mycobacterium ulcerans Agy99 4553833 YP_904831.1 CDS MUL_0706 NC_008611.1 740075 741016 D cytoplasmic protein; hypothetical protein 740075..741016 Mycobacterium ulcerans Agy99 4553834 YP_904832.1 CDS recD NC_008611.1 741037 742800 R cytoplasmic protein; involved in homologous recombination; exodeoxyribonuclease V (alpha chain), RecD complement(741037..742800) Mycobacterium ulcerans Agy99 4553835 YP_904833.1 CDS recB NC_008611.1 742797 746129 R cytoplasmic protein; involved in homologous recombination.; exodeoxyribonuclease V (beta chain), RecB complement(742797..746129) Mycobacterium ulcerans Agy99 4553836 YP_904834.1 CDS recC NC_008611.1 746129 749428 R cytoplasmic protein; involved in homologous recombination; exodeoxyribonuclease V (gamma chain), RecC complement(746129..749428) Mycobacterium ulcerans Agy99 4553837 YP_904835.1 CDS MUL_0710 NC_008611.1 749470 749919 D membrane protein; nitroreductase 749470..749919 Mycobacterium ulcerans Agy99 4553838 YP_904836.1 CDS cynS NC_008611.1 750066 750542 D catalyzes the reaction of cyanate and bicarbonate to produce ammonia and carbon dioxide; cyanate hydratase 750066..750542 Mycobacterium ulcerans Agy99 4553839 YP_904837.1 CDS echA3 NC_008611.1 751598 752293 R Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase complement(751598..752293) Mycobacterium ulcerans Agy99 4552571 YP_904838.1 CDS MUL_0714 NC_008611.1 752375 753247 R membrane protein; hypothetical protein complement(752375..753247) Mycobacterium ulcerans Agy99 4552572 YP_904839.1 CDS gloB NC_008611.1 753399 754115 R Detected in the cytoplasmic fraction by proteomics.; cytoplasmic protein; thought to be involved in glyoxal pathway. thioesterase that catalyses the hydrolysis of S-D-lactoyl- glutathione to form glutathione and D-lactic acid [catalytic activity: (S)-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy acid anion]; glyoxalase II, GloB complement(753399..754115) Mycobacterium ulcerans Agy99 4552573 YP_904840.1 CDS MUL_0716 NC_008611.1 754239 754448 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; domain homology to Arc/MetJ class transcription regulators; hypothetical protein 754239..754448 Mycobacterium ulcerans Agy99 4552574 YP_904841.1 CDS rpmG NC_008611.1 754836 755003 D in Escherichia coli BM108, a mutation that results in lack of L33 synthesis had no effect on ribosome synthesis or function; there are paralogous genes in several bacterial genomes, and a CXXC motif for zinc binding and an upstream regulation region of the paralog lacking this motif that are regulated by zinc similar to other ribosomal proteins like L31; the proteins in this group have the CXXC motif; 50S ribosomal protein L33 754836..755003 Mycobacterium ulcerans Agy99 4552577 YP_904842.1 CDS MUL_0720 NC_008611.1 755054 755533 D functions as a heterodimer along with HadB in fatty acid biosynthesis; fatty acid synthase type II; FAS-II; (3R)-hydroxyacyl-ACP dehydratase subunit HadA 755054..755533 Mycobacterium ulcerans Agy99 4552578 YP_904843.1 CDS MUL_0721 NC_008611.1 755520 755948 D functions as a heterodimer along with HadA or HadC in fatty acid biosynthesis; fatty acid synthase type II; FAS-II; (3R)-hydroxyacyl-ACP dehydratase subunit HadB 755520..755948 Mycobacterium ulcerans Agy99 4552579 YP_904844.1 CDS MUL_0722 NC_008611.1 755959 756459 D functions as a heterodimer along with HadB in fatty acid biosynthesis; fatty acid synthase type II; FAS-II; (3R)-hydroxyacyl-ACP dehydratase subunit HadC 755959..756459 Mycobacterium ulcerans Agy99 4552580 YP_904845.1 CDS secE NC_008611.1 756828 757316 D forms a complex with SecY and SecG; SecYEG forms a putative protein-conducting channel to which secA binds and translocates targeted polypeptides across the cytoplasmic membrane, a process driven by ATP and a proton-motive force; preprotein translocase subunit SecE 756828..757316 Mycobacterium ulcerans Agy99 4552582 YP_904846.1 CDS nusG NC_008611.1 757348 758061 D Modulates Rho-dependent transcription termination; transcription antitermination protein NusG 757348..758061 Mycobacterium ulcerans Agy99 4552583 YP_904847.1 CDS rplK NC_008611.1 758108 758536 D binds directly to 23S ribosomal RNA; 50S ribosomal protein L11 758108..758536 Mycobacterium ulcerans Agy99 4552584 YP_904848.1 CDS rplA NC_008611.1 758624 759331 D in Escherichia coli and Methanococcus, this protein autoregulates expression; the binding site in the mRNA mimics the binding site in the 23S rRNA; 50S ribosomal protein L1 758624..759331 Mycobacterium ulcerans Agy99 4552585 YP_904849.1 CDS mmaA4 NC_008611.1 761462 762400 R Detected in the cytoplamic fraction by LC-MS/MS. Detected in the membrane fraction by proteomics (2D-LC- MS/MS); cytoplasmic protein; methyltransferase. involved in mycolic acids modification. catalyzes unusual S-adenosyl-methionine- dependent transformation of a cis-olefin mycolic acid into a secondary alcohol. catalyzes introduction of a hydroxyl group at the distal position on mycolic acid chains to produce the hydroxyl mycolate. mycolic acids represent a major constituent of the mycobacterial cell wall complex. methyl transfer results in formation of a secondary hydroxy group with an adjacent methyl branch; olefinic mycolic acid methyl transferase.; methoxy mycolic acid synthase 4, MmaA4 complement(761462..762400) Mycobacterium ulcerans Agy99 4552128 YP_904850.1 CDS mmaA3 NC_008611.1 762504 763385 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; methyltransferase involved in mycolic acid biosynthesis; methoxy mycolic acid synthase 3, MmaA3 complement(762504..763385) Mycobacterium ulcerans Agy99 4552129 YP_904851.1 CDS mmaA2 NC_008611.1 763669 764385 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; methyltransferase involved in mycolic acid biosynthesis; methoxy mycolic acid synthase 2, MmaA2 complement(763669..764385) Mycobacterium ulcerans Agy99 4552130 YP_904852.1 CDS lipG1 NC_008611.1 765500 766444 R membrane protein; lipase/esterase complement(765500..766444) Mycobacterium ulcerans Agy99 4552132 YP_904853.1 CDS MUL_0734 NC_008611.1 766454 767869 R cytoplasmic protein; contains a kinase domain and ATP-binding cassette; hypothetical protein complement(766454..767869) Mycobacterium ulcerans Agy99 4552133 YP_904854.1 CDS lipG2 NC_008611.1 767959 768870 R cytoplasmic protein; lipase/esterase complement(767959..768870) Mycobacterium ulcerans Agy99 4552134 YP_904855.1 CDS MUL_0737 NC_008611.1 770468 774556 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; alpha-mannosidase activity: hydrolysis of terminal non-reducing alpha-D-mannose residues in alpha-D- mannosides.; alpha-mannosidase 770468..774556 Mycobacterium ulcerans Agy99 4552136 YP_904856.1 CDS fabD2 NC_008611.1 774578 775039 D cytoplasmic protein; nvolved in lipid metabolism; fatty acid biosynthesis [catalytic activity: malonyl-CoA + [acyl- carrier protein] = CoA + malonyl-[acyl-carrier protein]]; malonyl CoA-acyl carrier protein transacylase, FabD2 774578..775039 Mycobacterium ulcerans Agy99 4552137 YP_904857.1 CDS MUL_0739 NC_008611.1 775046 775954 D function unknown; probably involved in specific sugar metabolism or regulation; carbohydrate kinase 775046..775954 Mycobacterium ulcerans Agy99 4552138 YP_904858.1 CDS MUL_0740 NC_008611.1 776374 778350 D membrane protein; PE-PGRS family protein 776374..778350 Mycobacterium ulcerans Agy99 4552139 YP_904859.1 CDS rplJ NC_008611.1 778725 779345 D binds the two ribosomal protein L7/L12 dimers and anchors them to the large ribosomal subunit; 50S ribosomal protein L10 778725..779345 Mycobacterium ulcerans Agy99 4552140 YP_904860.1 CDS rplL NC_008611.1 779413 779805 D present in two forms; L12 is normal, while L7 is aminoacylated at the N-terminal serine; the only multicopy ribosomal protein; 4:1 ratio of L7/L12 per ribosome; two L12 dimers bind L10; critically important for translation efficiency and fidelity; stimulates GTPase activity of translation factors; 50S ribosomal protein L7/L12 779413..779805 Mycobacterium ulcerans Agy99 4552141 YP_904861.1 CDS MUL_0743 NC_008611.1 779869 780606 R cytoplasmic protein; function unknown, involved in transcriptional mechanism; transcriptional regulatory protein complement(779869..780606) Mycobacterium ulcerans Agy99 4552142 YP_904862.1 CDS MUL_0744 NC_008611.1 780678 782180 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS); cytoplasmic protein; dioxygenase and related enzymes [secondary metabolites biosynthesis, transport, and catabolism]; dioxygenase 780678..782180 Mycobacterium ulcerans Agy99 4552143 YP_904863.1 CDS mkl NC_008611.1 782359 783345 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS) Also detected in the cytoplasmic fraction by 2D- LC-MS/MS.; membrane protein; thought to be involved in active transport of ribonucleotide across the membrane. responsible for energy coupling to the transport system.; ribonucleotide-transport ATP-binding protein ABC transporter, Mkl 782359..783345 Mycobacterium ulcerans Agy99 4552144 YP_904864.1 CDS rpoB NC_008611.1 783938 787468 D DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates; beta subunit is part of the catalytic core which binds with a sigma factor to produce the holoenzyme; DNA-directed RNA polymerase subunit beta 783938..787468 Mycobacterium ulcerans Agy99 4552145 YP_904865.1 CDS rpoC NC_008611.1 787521 791471 D DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Subunit beta' binds to sigma factor allowing it to bind to the -10 region of the promoter; DNA-directed RNA polymerase subunit beta' 787521..791471 Mycobacterium ulcerans Agy99 4552035 YP_904866.1 CDS MUL_0748 NC_008611.1 792135 792326 D membrane protein; PE-PGRS family protein 792135..792326 Mycobacterium ulcerans Agy99 4552036 YP_904867.1 CDS end NC_008611.1 795440 796198 D Assists in DNA repair by cleaving phosphodiester bonds at apurinic or apyrimidinic sties to produce new 5' ends that are base-free deoxyribose 5-phosphate residues; endonuclease IV 795440..796198 Mycobacterium ulcerans Agy99 4552039 YP_904868.1 CDS lpqP NC_008611.1 796263 796919 D membrane protein; lipoprotein LpqP 796263..796919 Mycobacterium ulcerans Agy99 4552040 YP_904869.1 CDS MUL_0753 NC_008611.1 797119 798780 D cytoplasmic protein; may be involved in lipid degradation; acyl-CoA dehydrogenase 797119..798780 Mycobacterium ulcerans Agy99 4552041 YP_904870.1 CDS echA4 NC_008611.1 798789 799727 D Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase 798789..799727 Mycobacterium ulcerans Agy99 4552042 YP_904871.1 CDS MUL_0755 NC_008611.1 799741 800472 D membrane protein; high domain identity with transcriptional repressors; putative regulatory protein 799741..800472 Mycobacterium ulcerans Agy99 4552043 YP_904872.1 CDS echA5 NC_008611.1 800469 801242 D Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase 800469..801242 Mycobacterium ulcerans Agy99 4552044 YP_904873.1 CDS mmpL5 NC_008611.1 801299 804199 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; thought to be involved in fatty acid transport; transmembrane transport protein MmpL complement(801299..804199) Mycobacterium ulcerans Agy99 4552045 YP_904874.1 CDS mmpS5 NC_008611.1 804196 804624 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; transmembrane proteinm, MmpS5 complement(804196..804624) Mycobacterium ulcerans Agy99 4552046 YP_904875.1 CDS MUL_0759 NC_008611.1 804808 805308 D cytoplasmic protein; transcriptional regulator 804808..805308 Mycobacterium ulcerans Agy99 4552047 YP_904876.1 CDS MUL_0760 NC_008611.1 805358 805747 R membrane protein; hypothetical protein complement(805358..805747) Mycobacterium ulcerans Agy99 4552048 YP_904877.1 CDS MUL_0761 NC_008611.1 805885 806262 R membrane protein; hypothetical protein complement(805885..806262) Mycobacterium ulcerans Agy99 4552049 YP_904878.1 CDS MUL_0762 NC_008611.1 806526 807173 D cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator 806526..807173 Mycobacterium ulcerans Agy99 4552050 YP_904879.1 CDS rpsL NC_008611.1 807549 807923 D interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone; located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side; mutations in the S12 gene confer streptomycin resistance; 30S ribosomal protein S12 807549..807923 Mycobacterium ulcerans Agy99 4552051 YP_904880.1 CDS rpsG NC_008611.1 807923 808393 D binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit; 30S ribosomal protein S7 807923..808393 Mycobacterium ulcerans Agy99 4552052 YP_904881.1 CDS fusA1 NC_008611.1 808474 810579 D EF-G; promotes GTP-dependent translocation of the ribosome during translation; many organisms have multiple copies of this gene; elongation factor G 808474..810579 Mycobacterium ulcerans Agy99 4552053 YP_904882.1 CDS tuf NC_008611.1 810766 811956 D EF-Tu; promotes GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis; when the tRNA anticodon matches the mRNA codon, GTP hydrolysis results; the inactive EF-Tu-GDP leaves the ribosome and release of GDP is promoted by elongation factor Ts; many prokaryotes have two copies of the gene encoding EF-Tu; elongation factor Tu 810766..811956 Mycobacterium ulcerans Agy99 4551894 YP_904883.1 CDS MUL_0767 NC_008611.1 812206 813015 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 812206..813015 Mycobacterium ulcerans Agy99 4551895 YP_904884.1 CDS MUL_0768 NC_008611.1 813228 813974 D Catalyzes the first of the two reduction steps in the elongation cycle of fatty acid synthesis; 3-ketoacyl-ACP reductase 813228..813974 Mycobacterium ulcerans Agy99 4551896 YP_904885.1 CDS MUL_0769 NC_008611.1 813984 815414 D cytoplasmic protein; ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.; ferredoxin reductase 813984..815414 Mycobacterium ulcerans Agy99 4551897 YP_904886.1 CDS MUL_0770 NC_008611.1 815320 816381 R cytoplasmic protein; hypothetical protein complement(815320..816381) Mycobacterium ulcerans Agy99 4551898 YP_904887.1 CDS MUL_0771 NC_008611.1 816381 816974 R cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulator complement(816381..816974) Mycobacterium ulcerans Agy99 4551899 YP_904888.1 CDS MUL_0772 NC_008611.1 817173 817502 D hypothetical protein 817173..817502 Mycobacterium ulcerans Agy99 4551900 YP_904889.1 CDS pqqE NC_008611.1 817499 818608 D cytoplasmic protein; required for coenzyme pyrrolo-quinoline-quinone (PQQ) biosynthesis.; coenzyme PQQ synthesis protein E, PqqE 817499..818608 Mycobacterium ulcerans Agy99 4551901 YP_904890.1 CDS lldD1 NC_008611.1 818679 819851 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in respiration; catalyzes conversion of lactate into pyruvate [catalytic activity: (S)-lactate + 2 ferricytochrome C = pyruvate + 2 ferrocytochrome C]; L-lactate dehydrogenase (cytochrome) LldD1 818679..819851 Mycobacterium ulcerans Agy99 4551902 YP_904891.1 CDS MUL_0775 NC_008611.1 820006 820758 D cytoplasmic protein; catalyses the hydrolysis of creatinine to creatine; creatinine amidohydrolase 820006..820758 Mycobacterium ulcerans Agy99 4551903 YP_904892.1 CDS MUL_0776 NC_008611.1 820755 822167 D membrane protein; membrane glycosyl transferase 820755..822167 Mycobacterium ulcerans Agy99 4551904 YP_904893.1 CDS MUL_0777 NC_008611.1 822176 823612 D membrane protein; dehydrogenase 822176..823612 Mycobacterium ulcerans Agy99 4551905 YP_904894.1 CDS MUL_0778 NC_008611.1 823602 824255 R cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator complement(823602..824255) Mycobacterium ulcerans Agy99 4551906 YP_904895.1 CDS MUL_0780 NC_008611.1 826603 827946 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(826603..827946) Mycobacterium ulcerans Agy99 4551908 YP_904896.1 CDS MUL_0781 NC_008611.1 828377 829381 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 828377..829381 Mycobacterium ulcerans Agy99 4551909 YP_904897.1 CDS MUL_0782 NC_008611.1 829924 831396 D cytoplasmic protein; C-term truncated by IS2606 insertion; PPE family protein 829924..831396 Mycobacterium ulcerans Agy99 4551910 YP_904898.1 CDS MUL_0784 NC_008611.1 832513 832830 R cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 complement(832513..832830) Mycobacterium ulcerans Agy99 4551528 YP_904899.1 CDS MUL_0785 NC_008611.1 832855 833559 R cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 complement(832855..833559) Mycobacterium ulcerans Agy99 4551529 YP_904900.1 CDS MUL_0787 NC_008611.1 834619 835986 D membrane protein; lipase 834619..835986 Mycobacterium ulcerans Agy99 4551531 YP_904901.1 CDS MUL_0788 NC_008611.1 835919 836239 D cytoplasmic protein; hypothetical protein 835919..836239 Mycobacterium ulcerans Agy99 4551532 YP_904902.1 CDS rpsJ NC_008611.1 836530 836835 D NusE; involved in assembly of the 30S subunit; in the ribosome, this protein is involved in the binding of tRNA; in Escherichia coli this protein was also found to be involved in transcription antitermination; NusB/S10 heterodimers bind boxA sequences in the leader RNA of rrn operons which is required for antitermination; binding of NusB/S10 to boxA nucleates assembly of the antitermination complex; 30S ribosomal protein S10 836530..836835 Mycobacterium ulcerans Agy99 4551533 YP_904903.1 CDS rplC NC_008611.1 836850 837503 D binds directly near the 3' end of the 23S rRNA, where it nucleates assembly of the 50S subunit; essential for peptidyltransferase activity; mutations in this gene confer resistance to tiamulin; 50S ribosomal protein L3 836850..837503 Mycobacterium ulcerans Agy99 4551534 YP_904904.1 CDS rplD NC_008611.1 837503 838177 D L4 is important during the early stages of 50S assembly; it initially binds near the 5' end of the 23S rRNA; 50S ribosomal protein L4 837503..838177 Mycobacterium ulcerans Agy99 4551535 YP_904905.1 CDS rplW NC_008611.1 838177 838479 D binds third domain of 23S rRNA and protein L29; part of exit tunnel; 50S ribosomal protein L23 838177..838479 Mycobacterium ulcerans Agy99 4551536 YP_904906.1 CDS rplB NC_008611.1 838509 839351 D one of the primary rRNA-binding proteins; required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation; 50S ribosomal protein L2 838509..839351 Mycobacterium ulcerans Agy99 4551537 YP_904907.1 CDS rpsS NC_008611.1 839373 839654 D protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA; 30S ribosomal protein S19 839373..839654 Mycobacterium ulcerans Agy99 4551538 YP_904908.1 CDS rplV NC_008611.1 839683 840213 D binds specifically to 23S rRNA during the early stages of 50S assembly; makes contact with all 6 domains of the 23S rRNA in the assembled 50S subunit and ribosome; mutations in this gene result in erythromycin resistance; located near peptidyl-transferase center; 50S ribosomal protein L22 839683..840213 Mycobacterium ulcerans Agy99 4551539 YP_904909.1 CDS rpsC NC_008611.1 840213 841040 D forms a complex with S10 and S14; binds the lower part of the 30S subunit head and the mRNA in the complete ribosome to position it for translation; 30S ribosomal protein S3 840213..841040 Mycobacterium ulcerans Agy99 4551540 YP_904910.1 CDS rplP NC_008611.1 841044 841460 D located in the peptidyl transferase center and may be involved in peptidyl transferase activity; similar to archaeal L10e; 50S ribosomal protein L16 841044..841460 Mycobacterium ulcerans Agy99 4551541 YP_904911.1 CDS rpmC NC_008611.1 841460 841702 D one of the stabilizing components for the large ribosomal subunit; 50S ribosomal protein L29 841460..841702 Mycobacterium ulcerans Agy99 4551542 YP_904912.1 CDS rpsQ NC_008611.1 841699 842061 D primary binding protein; helps mediate assembly; involved in translation fidelity; 30S ribosomal protein S17 841699..842061 Mycobacterium ulcerans Agy99 4551543 YP_904913.1 CDS MUL_0801 NC_008611.1 844636 845247 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 844636..845247 Mycobacterium ulcerans Agy99 4551338 YP_904914.1 CDS MUL_0803 NC_008611.1 846265 847203 D membrane protein; transmembrane protein 846265..847203 Mycobacterium ulcerans Agy99 4551340 YP_904915.1 CDS rplN NC_008611.1 847454 847822 D binds to the 23S rRNA between the centers for peptidyl transferase and GTPase; 50S ribosomal protein L14 847454..847822 Mycobacterium ulcerans Agy99 4551341 YP_904916.1 CDS rplX NC_008611.1 847823 848146 D assembly initiator protein; binds to 5' end of 23S rRNA and nucleates assembly of the 50S; surrounds polypeptide exit tunnel; 50S ribosomal protein L24 847823..848146 Mycobacterium ulcerans Agy99 4551342 YP_904917.1 CDS rplE NC_008611.1 848143 848715 D part of 50S and 5S/L5/L18/L25 subcomplex; contacts 5S rRNA and P site tRNA; forms a bridge to the 30S subunit in the ribosome by binding to S13; 50S ribosomal protein L5 848143..848715 Mycobacterium ulcerans Agy99 4551343 YP_904918.1 CDS rpsN NC_008611.1 848720 848905 D located in the peptidyl transferase center and involved in assembly of 30S ribosome subunit; similar to what is observed with proteins L31 and L33, some proteins in this family contain CXXC motifs that are involved in zinc binding; if two copies are present in a genome, then the duplicated copy appears to have lost the zinc-binding motif and is instead regulated by zinc; the proteins in this group appear to contain the zinc-binding motif; 30S ribosomal protein S14 848720..848905 Mycobacterium ulcerans Agy99 4551344 YP_904919.1 CDS rpsH NC_008611.1 848982 849380 D binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit; 30S ribosomal protein S8 848982..849380 Mycobacterium ulcerans Agy99 4551345 YP_904920.1 CDS rplF NC_008611.1 849391 849930 D ribosomal protein L6 appears to have arisen as a result of an ancient gene duplication as based on structural comparison of the Bacillus stearothermophilus protein; RNA-binding appears to be in the C-terminal domain; mutations in the L6 gene confer resistance to aminoglycoside antibiotics such as gentamicin and these occur in truncations of the C-terminal domain; it has been localized to a region between the base of the L7/L12 stalk and the central protuberance; 50S ribosomal protein L6 849391..849930 Mycobacterium ulcerans Agy99 4551346 YP_904921.1 CDS rplR NC_008611.1 849972 850340 D cytoplasmic protein; this is one of 3 proteins that mediate the attachment of the 5S RNA into the large ribosomal subunit.; 50S ribosomal protein L18 849972..850340 Mycobacterium ulcerans Agy99 4551347 YP_904922.1 CDS rpsE NC_008611.1 850378 851052 D located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body; contacts S4 and S8; with S4 and S12 plays a role in translational accuracy; mutations in this gene result in spectinomycin resistance; 30S ribosomal protein S5 850378..851052 Mycobacterium ulcerans Agy99 4551348 YP_904923.1 CDS rpmD NC_008611.1 851049 851267 D L30 binds domain II of the 23S rRNA and the 5S rRNA; similar to eukaryotic protein L7; 50S ribosomal protein L30 851049..851267 Mycobacterium ulcerans Agy99 4551349 YP_904924.1 CDS rplO NC_008611.1 851264 851704 D late assembly protein; 50S ribosomal protein L15 851264..851704 Mycobacterium ulcerans Agy99 4551350 YP_904925.1 CDS MUL_0814 NC_008611.1 851772 852698 R cytoplasmic protein; flavin-dependent oxidoreductase complement(851772..852698) Mycobacterium ulcerans Agy99 4551351 YP_904926.1 CDS ssp NC_008611.1 852785 854587 D membrane protein; involved in digestion of the cleaved signal peptides. this activity is necessary to maintain proper secretion of mature proteins across the membrane.; protease IV, Ssp 852785..854587 Mycobacterium ulcerans Agy99 4551352 YP_904927.1 CDS MUL_0816 NC_008611.1 854584 855498 R cytoplasmic protein; O-methyltransferase complement(854584..855498) Mycobacterium ulcerans Agy99 4551353 YP_904928.1 CDS MUL_0817 NC_008611.1 855518 856420 R cytoplasmic protein; O-methyltransferase complement(855518..856420) Mycobacterium ulcerans Agy99 4551354 YP_904929.1 CDS MUL_0818 NC_008611.1 856417 857391 R cytoplasmic protein; O-methyltransferase complement(856417..857391) Mycobacterium ulcerans Agy99 4551355 YP_904930.1 CDS adh_1 NC_008611.1 857608 859623 D membrane protein; function unknown, may contain two dehyrogenase domains, first showing better similarity; short-chain dehydrogenase 857608..859623 Mycobacterium ulcerans Agy99 4551356 YP_904931.1 CDS MUL_0820 NC_008611.1 859632 860312 R cytoplasmic protein; methyltransferase complement(859632..860312) Mycobacterium ulcerans Agy99 4551357 YP_904932.1 CDS MUL_0821 NC_008611.1 860368 861246 D cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein 860368..861246 Mycobacterium ulcerans Agy99 4550173 YP_904933.1 CDS fucA NC_008611.1 861270 861926 R catalyzes the formation of glycerone phosphate and (S)-lactaldehyde from L-fuculose 1-phosphate; L-fuculose phosphate aldolase complement(861270..861926) Mycobacterium ulcerans Agy99 4550174 YP_904934.1 CDS serA2 NC_008611.1 861923 862912 R cytoplasmic protein; involved at the first committed step in the 'phosphorylated' pathway of L-serine biosynthesis. catalyzes the oxidation of D-3-phosphoglycerate to 3- phosphohydroxypyruvate [catalytic activity: 3- phosphoglycerate + NAD(+) = 3-phosphohydroxypyruvate + NADH]; D-3-phosphoglycerate dehydrogenase complement(861923..862912) Mycobacterium ulcerans Agy99 4550175 YP_904935.1 CDS xylB NC_008611.1 862943 864178 D membrane protein; phosphorylates D-xylulose [catalytic activity: ATP + D-xylulose = ADP + D-xylulose 5-phosphate]; D-xylulose-kinase (xylulokinase), XylB 862943..864178 Mycobacterium ulcerans Agy99 4550176 YP_904936.1 CDS MUL_0825 NC_008611.1 864386 865114 D Detected in the cytoplasmic and membrane fractions by LC-MS/MS. Also detected in the extracellular matrix by proteomics.; cytoplasmic protein; hypothetical protein 864386..865114 Mycobacterium ulcerans Agy99 4550177 YP_904937.1 CDS secY NC_008611.1 867306 868631 D forms heterotrimeric complex in the membrane; in bacteria the complex consists of SecY which forms the channel pore and SecE and SecG; the SecG subunit is not essential; in bacteria translocation is driven via the SecA ATPase; preprotein translocase subunit SecY 867306..868631 Mycobacterium ulcerans Agy99 4550180 YP_904938.1 CDS adk NC_008611.1 868628 869173 D essential enzyme that recycles AMP in active cells; converts ATP and AMP to two molecules of ADP; adenylate kinase 868628..869173 Mycobacterium ulcerans Agy99 4550181 YP_904939.1 CDS mapA NC_008611.1 869176 869976 D catalyzes the removal of N-terminal amino acids from peptides and arylamides; generally Co(II) however activity has been shown for some methionine aminopeptidases with Zn, Fe, or Mn; methionine aminopeptidase 869176..869976 Mycobacterium ulcerans Agy99 4550182 YP_904940.1 CDS sigL NC_008611.1 870029 870562 D Member of the extracytoplasmic function sigma factors which are active under specific conditions; binds with the catalytic core of RNA polymerase to produce the holoenzyme and directs bacterial core RNA polymerase to specific promoter elements to initiate transcription; in M. tuberculosis this protein regulates polyketide synthases and secreted or membrane proteins; RNA polymerase sigma factor SigL 870029..870562 Mycobacterium ulcerans Agy99 4550183 YP_904941.1 CDS MUL_0832 NC_008611.1 870641 871387 D membrane protein; function unknown, domain identity suggests possible role in transcription regulation; hypothetical protein 870641..871387 Mycobacterium ulcerans Agy99 4550184 YP_904942.1 CDS MUL_0833 NC_008611.1 871474 871974 D cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulator 871474..871974 Mycobacterium ulcerans Agy99 4550185 YP_904943.1 CDS MUL_0834 NC_008611.1 871998 872546 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein 871998..872546 Mycobacterium ulcerans Agy99 4550186 YP_904944.1 CDS mmsB NC_008611.1 872559 873443 R membrane protein; catalyzes the NAD-dependent, reversible oxidation of 3-hydroxbutyrate to methylmalonate [catalytic activity: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3- oxopropanoate + NADH]; 3-hydroxyisobutyrate dehydrogenase complement(872559..873443) Mycobacterium ulcerans Agy99 4550187 YP_904945.1 CDS fadE9 NC_008611.1 873456 874628 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; lipid metabolism; acyl-CoA dehydrogenase, FadE9 complement(873456..874628) Mycobacterium ulcerans Agy99 4550188 YP_904946.1 CDS mmsA NC_008611.1 874634 876154 R Detected in the cytoplamic fraction by LCMSMS. Also detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; plays a role in valine and pyrimidine metabolism. binds fatty acyl-CoA [catalytic activity: 2-methyl-3- oxopropanoate + CoA + NAD+ = propanoyl-CoA + CO2 + NADH]; methylmalonate semialdehyde dehydrogenase, MmsA complement(874634..876154) Mycobacterium ulcerans Agy99 4550189 YP_904947.1 CDS MUL_0838 NC_008611.1 876334 877914 R membrane protein; transmembrane carbohydrate transport protein complement(876334..877914) Mycobacterium ulcerans Agy99 4550190 YP_904948.1 CDS rmlC NC_008611.1 878058 878663 R cytoplasmic protein; involved in dTDP-L-rhamnose biosynthesis, within the O antigen biosynthesis pathway of lipopolysaccharide biosynthesis: conversion of dTDP-4-keto-6-deoxy-D-glucose to dTDP-4-keto-rhamnose [catalytic activity: dTDP-4- dehydro-6-deoxy-D-glucose = dTDP-4-dehydro-6-deoxy-L- mannose]; dTDP-4-dehydrorhamnose 3,5-epimerase, RmlC complement(878058..878663) Mycobacterium ulcerans Agy99 4550191 YP_904949.1 CDS rmlB NC_008611.1 878665 879660 R cytoplasmic protein; involved in dTDP-L-rhamnose biosynthesis [catalytic activity: dTDP-glucose = DTDP-4-dehydro-6-deoxy-D-glucose + H(2)O]; dTDP-glucose-4,6-dehydratase, RmlB complement(878665..879660) Mycobacterium ulcerans Agy99 4553550 YP_904950.1 CDS MUL_0841 NC_008611.1 879670 880530 R cytoplasmic protein; hypothetical protein complement(879670..880530) Mycobacterium ulcerans Agy99 4553551 YP_904951.1 CDS MUL_0842 NC_008611.1 880557 881453 R cytoplasmic protein; hypothetical protein complement(880557..881453) Mycobacterium ulcerans Agy99 4553552 YP_904952.1 CDS infA NC_008611.1 881701 881922 D stimulates the activities of the other two initiation factors, IF-2 and IF-3; translation initiation factor IF-1 881701..881922 Mycobacterium ulcerans Agy99 4553553 YP_904953.1 CDS rpmJ NC_008611.1 881956 882069 D smallest protein in the large subunit; similar to what is found with protein L31 and L33 several bacterial genomes contain paralogs which may be regulated by zinc; the protein from Thermus thermophilus has a zinc-binding motif and contains a bound zinc ion; the proteins in this group have the motif; 50S ribosomal protein L36 881956..882069 Mycobacterium ulcerans Agy99 4553554 YP_904954.1 CDS rpsM NC_008611.1 882260 882634 D located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA; makes contact with the large subunit via RNA-protein interactions and via protein-protein interactions with L5; contacts P-site tRNA; 30S ribosomal protein S13 882260..882634 Mycobacterium ulcerans Agy99 4553555 YP_904955.1 CDS rpsK NC_008611.1 882638 883054 D located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA; forms part of the Shine-Dalgarno cleft in the 70S ribosome; interacts with S7 and S18 and IF-3; 30S ribosomal protein S11 882638..883054 Mycobacterium ulcerans Agy99 4553556 YP_904956.1 CDS rpsD NC_008611.1 883073 883678 D primary rRNA binding protein; nucleates 30S assembly; involved in translational accuracy with proteins S5 and S12; interacts with protein S5; involved in autogeneously regulating ribosomal proteins by binding to pseudoknot structures in the polycistronic mRNA; interacts with transcription complex and functions similar to protein NusA in antitermination; 30S ribosomal protein S4 883073..883678 Mycobacterium ulcerans Agy99 4553557 YP_904957.1 CDS rpoA NC_008611.1 883764 884807 D catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Dimerization of the alpha subunit is the first step in the sequential assembly of subunits to form the holoenzyme; DNA-directed RNA polymerase subunit alpha 883764..884807 Mycobacterium ulcerans Agy99 4553558 YP_904958.1 CDS rplQ NC_008611.1 884868 885437 D is a component of the macrolide binding site in the peptidyl transferase center; 50S ribosomal protein L17 884868..885437 Mycobacterium ulcerans Agy99 4553559 YP_904959.1 CDS truA NC_008611.1 885499 886281 D mediates pseudouridylation (positions 38, 39, 40) at the tRNA anticodon region which contributes to the structural stability; tRNA pseudouridine synthase A 885499..886281 Mycobacterium ulcerans Agy99 4553560 YP_904960.1 CDS cycA NC_008611.1 886297 887787 R membrane protein; permease that is involved in the transport across the cytoplasmic membrane of D-alanine, D-serine and glycine; D-serine/D-alanine/glycine transporter, CycA complement(886297..887787) Mycobacterium ulcerans Agy99 4553561 YP_904961.1 CDS MUL_5078 NC_008611.1 888055 889197 R membrane protein; PPE family protein complement(888055..889197) Mycobacterium ulcerans Agy99 4553562 YP_904962.1 CDS cut4 NC_008611.1 892507 893202 R membrane protein; hydrolysis of cutin (a polyester that forms the structure of plant cuticle); cutinase complement(892507..893202) Mycobacterium ulcerans Agy99 4553565 YP_904963.1 CDS cut3 NC_008611.1 893234 894037 R membrane protein; cutinase complement(893234..894037) Mycobacterium ulcerans Agy99 4553566 YP_904964.1 CDS MUL_0857 NC_008611.1 894150 895658 D membrane protein; hypothetical protein 894150..895658 Mycobacterium ulcerans Agy99 4553567 YP_904965.1 CDS mycP4 NC_008611.1 895548 896921 R membrane protein; membrane-anchored serine protease (mycosin), MycP4 complement(895548..896921) Mycobacterium ulcerans Agy99 4553568 YP_904966.1 CDS MUL_0859 NC_008611.1 896918 898300 R membrane protein; hypothetical protein complement(896918..898300) Mycobacterium ulcerans Agy99 4553765 YP_904967.1 CDS MUL_0860 NC_008611.1 898415 902095 D membrane protein; function unknown but has 3 ATP binding P-loop motifs; hypothetical protein 898415..902095 Mycobacterium ulcerans Agy99 4553766 YP_904968.1 CDS MUL_0861 NC_008611.1 902092 903336 D membrane protein; hypothetical protein 902092..903336 Mycobacterium ulcerans Agy99 4553767 YP_904969.1 CDS esxU NC_008611.1 903399 903752 D secreted protein; EsaT-6 like protein, EsxU 903399..903752 Mycobacterium ulcerans Agy99 4553768 YP_904970.1 CDS esxT NC_008611.1 903768 904064 D secreted protein; EsaT-6 like protein, EsxT 903768..904064 Mycobacterium ulcerans Agy99 4553769 YP_904971.1 CDS rplM NC_008611.1 904303 904746 D in Escherichia coli this protein is one of the earliest assembly proteins in the large subunit; 50S ribosomal protein L13 904303..904746 Mycobacterium ulcerans Agy99 4553770 YP_904972.1 CDS rpsI NC_008611.1 904743 905201 D forms a direct contact with the tRNA during translation; 30S ribosomal protein S9 904743..905201 Mycobacterium ulcerans Agy99 4553771 YP_904973.1 CDS glmM NC_008611.1 905352 906689 D catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate; phosphoglucosamine mutase 905352..906689 Mycobacterium ulcerans Agy99 4553772 YP_904974.1 CDS MUL_0867 NC_008611.1 906794 907105 D cytoplasmic protein; hypothetical protein 906794..907105 Mycobacterium ulcerans Agy99 4553773 YP_904975.1 CDS MUL_0868 NC_008611.1 907108 908505 D cytoplasmic protein; hypothetical protein 907108..908505 Mycobacterium ulcerans Agy99 4553774 YP_904976.1 CDS MUL_0870 NC_008611.1 909590 910429 R Detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; hydrolase domain identity; hypothetical protein complement(909590..910429) Mycobacterium ulcerans Agy99 4553776 YP_904977.1 CDS glmS NC_008611.1 911022 912899 D Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source; glucosamine--fructose-6-phosphate aminotransferase 911022..912899 Mycobacterium ulcerans Agy99 4553778 YP_904978.1 CDS MUL_0874 NC_008611.1 913561 914607 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(913561..914607) Mycobacterium ulcerans Agy99 4553780 YP_904979.1 CDS MUL_0876 NC_008611.1 915234 915947 D hypothetical protein 915234..915947 Mycobacterium ulcerans Agy99 4553781 YP_904980.1 CDS MUL_0877 NC_008611.1 915978 917399 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; has domain identity with YjeF-related protein N- terminus and sugar kinases c-terminus; transmembrane protein 915978..917399 Mycobacterium ulcerans Agy99 4553782 YP_904981.1 CDS gadB NC_008611.1 917436 918821 D cytoplasmic protein; catalyzes the production of GabA [catalytic activity: L-glutamate = 4-aminobutanoate + CO(2)]; glutamate decarboxylase 917436..918821 Mycobacterium ulcerans Agy99 4553783 YP_904982.1 CDS alr NC_008611.1 918908 920074 D converts L-alanine to D-alanine which is used in cell wall biosynthesis; binds one pyridoxal phosphate per monomer; forms a homodimer; alanine racemase 918908..920074 Mycobacterium ulcerans Agy99 4550853 YP_904983.1 CDS MUL_0880 NC_008611.1 920064 921185 D secreted protein; hydrolase 920064..921185 Mycobacterium ulcerans Agy99 4550854 YP_904984.1 CDS MUL_0881 NC_008611.1 921178 921648 D cytoplasmic protein; ATPase domain; hypothetical protein 921178..921648 Mycobacterium ulcerans Agy99 4550855 YP_904985.1 CDS MUL_0882 NC_008611.1 921645 922271 D membrane protein; peptidase; hypothetical protein 921645..922271 Mycobacterium ulcerans Agy99 4550856 YP_904986.1 CDS rimI NC_008611.1 922268 922744 D cytoplasmic protein; this enzyme acetylates the N-terminal alanine of ribosomal protein S18 [catalytic activity: acetyl-CoA + ribosomal-protein L-alanine = CoA + ribosomal-protein N- acetyl-L-alanine]; ribosomal-protein-alanine acetyltransferase, RimI 922268..922744 Mycobacterium ulcerans Agy99 4550857 YP_904987.1 CDS gcp NC_008611.1 922745 923776 D in most organisms, only the N-terminal domain is present in a single polypeptide; in some archaea this domain is fused to a kinase domain; this gene is essential for growth in Escherichia coli and Bacillus subtilis; the secreted glycoprotease from Pasteurella haemolytica showed specificity for O-sialoglycosylated proteins; the Pyrococcus structure shows DNA-binding properties, iron-binding, ATP-binding, and AP endonuclease activity; putative DNA-binding/iron metalloprotein/AP endonuclease 922745..923776 Mycobacterium ulcerans Agy99 4550858 YP_904988.1 CDS groES NC_008611.1 924090 924392 D 10 kDa chaperonin; Cpn10; GroES; forms homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring; co-chaperonin GroES 924090..924392 Mycobacterium ulcerans Agy99 4550859 YP_904989.1 CDS groEL NC_008611.1 924485 926104 D 60 kDa chaperone family; promotes refolding of misfolded polypeptides especially under stressful conditions; forms two stacked rings of heptamers to form a barrel-shaped 14mer; ends can be capped by GroES; misfolded proteins enter the barrel where they are refolded when GroES binds; many bacteria have multiple copies of the groEL gene which are active under different environmental conditions; the B.japonicum protein in this cluster is expressed constitutively; in Rhodobacter, Corynebacterium and Rhizobium this protein is not essential for growth; chaperonin GroEL 924485..926104 Mycobacterium ulcerans Agy99 4550860 YP_904990.1 CDS MUL_0888 NC_008611.1 927247 928293 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(927247..928293) Mycobacterium ulcerans Agy99 4550862 YP_904991.1 CDS MUL_0889 NC_008611.1 928988 929425 R cytoplasmic protein; hypothetical protein complement(928988..929425) Mycobacterium ulcerans Agy99 4550863 YP_904992.1 CDS MUL_0890 NC_008611.1 929692 931407 R membrane protein; PPE family protein complement(929692..931407) Mycobacterium ulcerans Agy99 4550864 YP_904993.1 CDS MUL_0891 NC_008611.1 931450 931977 D cytoplasmic protein; hypothetical protein 931450..931977 Mycobacterium ulcerans Agy99 4550865 YP_904994.1 CDS MUL_0894 NC_008611.1 933955 935610 D cytoplasmic protein; metal-dependent hydrolase 933955..935610 Mycobacterium ulcerans Agy99 4550867 YP_904995.1 CDS whiB3 NC_008611.1 935655 935963 R cytoplasmic protein; involved in transcriptional mechanism (growth phase- dependent); Whib-like regulatory protein, WhiB3 complement(935655..935963) Mycobacterium ulcerans Agy99 4550868 YP_904996.1 CDS MUL_0896 NC_008611.1 936474 937520 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 936474..937520 Mycobacterium ulcerans Agy99 4550869 YP_904997.1 CDS sigD NC_008611.1 937567 938160 D Member of the extracytoplasmic function sigma factors which are active under specific conditions; binds with the catalytic core of RNA polymerase to produce the holoenzyme and directs bacterial core RNA polymerase to specific promoter elements to initiate transcription; this protein is involved in expression of ribosome-associated gene products in stationary phase; RNA polymerase sigma factor SigD 937567..938160 Mycobacterium ulcerans Agy99 4550780 YP_904998.1 CDS MUL_0898 NC_008611.1 938153 939199 D cytoplasmic protein; hypothetical protein 938153..939199 Mycobacterium ulcerans Agy99 4550781 YP_904999.1 CDS MUL_0899 NC_008611.1 939260 939673 R cytoplasmic protein; hypothetical protein complement(939260..939673) Mycobacterium ulcerans Agy99 4550782 YP_905000.1 CDS guaB2 NC_008611.1 939837 941435 D catalyzes the synthesis of xanthosine monophosphate by the NAD+ dependent oxidation of inosine monophosphate; inosine 5'-monophosphate dehydrogenase 939837..941435 Mycobacterium ulcerans Agy99 4550783 YP_905001.1 CDS guaB3 NC_008611.1 941445 942572 D catalyzes the synthesis of xanthosine monophosphate by the NAD+ dependent oxidation of inosine monophosphate; inosine 5-monophosphate dehydrogenase 941445..942572 Mycobacterium ulcerans Agy99 4550784 YP_905002.1 CDS MUL_0902 NC_008611.1 942753 944579 D membrane protein; PPE family protein 942753..944579 Mycobacterium ulcerans Agy99 4550785 YP_905003.1 CDS choD NC_008611.1 944668 946413 D membrane protein; involved in cholesterol metabolism [catalytic activity: cholesterol + O(2) = cholest-4-en-3-one + H(2)O(2)]; cholesterol oxidase ChoD 944668..946413 Mycobacterium ulcerans Agy99 4550786 YP_905004.1 CDS tauD NC_008611.1 946439 947335 R cytoplasmic protein; in E. coli this enzyme catalyses the oxygenolytic release of sulfite from taurine.; taurine catabolism dioxygenase, TauD complement(946439..947335) Mycobacterium ulcerans Agy99 4550787 YP_905005.1 CDS MUL_0905 NC_008611.1 947396 947971 D cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulator 947396..947971 Mycobacterium ulcerans Agy99 4550788 YP_905006.1 CDS MUL_0906 NC_008611.1 947957 949873 R cytoplasmic protein; similar to maltose phosphorylase, a dimeric enzyme that catalyses the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose; glycosyl hydrolase complement(947957..949873) Mycobacterium ulcerans Agy99 4550789 YP_905007.1 CDS MUL_0907 NC_008611.1 950296 951342 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 950296..951342 Mycobacterium ulcerans Agy99 4550790 YP_905008.1 CDS MUL_0908 NC_008611.1 951308 951865 R cytoplasmic protein; cellular metabolism; hydrolase complement(951308..951865) Mycobacterium ulcerans Agy99 4550791 YP_905009.1 CDS MUL_0909 NC_008611.1 951947 952465 D cytoplasmic protein; hypothetical protein 951947..952465 Mycobacterium ulcerans Agy99 4550792 YP_905010.1 CDS MUL_0910 NC_008611.1 952504 953121 D cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein 952504..953121 Mycobacterium ulcerans Agy99 4550793 YP_905011.1 CDS MUL_0911 NC_008611.1 953118 954674 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS) Also detected in the cytoplasmic fraction by 2D- LC-MS/MS.; membrane protein; integral membrane transport protein complement(953118..954674) Mycobacterium ulcerans Agy99 4550794 YP_905012.1 CDS MUL_0912 NC_008611.1 954753 955436 D cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein 954753..955436 Mycobacterium ulcerans Agy99 4550795 YP_905013.1 CDS guaA NC_008611.1 955509 957092 D contains glutamine-hydrolyzing domain and glutamine amidotransferase; GMP-binding domain; functions to produce GMP from XMP in the IMP pathway; GMP synthase 955509..957092 Mycobacterium ulcerans Agy99 4550796 YP_905014.1 CDS MUL_0914 NC_008611.1 957160 957849 R secreted protein; hypothetical protein complement(957160..957849) Mycobacterium ulcerans Agy99 4550797 YP_905015.1 CDS MUL_0915 NC_008611.1 957923 958615 D cytoplasmic protein; hypothetical protein 957923..958615 Mycobacterium ulcerans Agy99 4550798 YP_905016.1 CDS MUL_0916 NC_008611.1 958622 960193 D secreted protein; function unknown but belongs to the DNA/RNA polymerase superfamily; hypothetical protein 958622..960193 Mycobacterium ulcerans Agy99 4551638 YP_905017.1 CDS acrA1 NC_008611.1 961261 963273 R cytoplasmic protein; multi functional protein with fatty acyl-CoA reductase activity in C-terminal part; short chain dehydrogenase complement(961261..963273) Mycobacterium ulcerans Agy99 4551640 YP_905018.1 CDS lpqD NC_008611.1 963313 964026 R membrane protein; lipoprotein LpqD complement(963313..964026) Mycobacterium ulcerans Agy99 4551641 YP_905019.1 CDS MUL_0920 NC_008611.1 964122 964994 D Also detected as secreted protein; cytoplasmic protein; may have dehydratase activity; contains a MaoC protein family domain; hypothetical protein 964122..964994 Mycobacterium ulcerans Agy99 4551642 YP_905020.1 CDS otsB2 NC_008611.1 965008 966180 R cytoplasmic protein; involved in osmoregulatory trehalose biosynthesis. mycobacteria can produce trehalose from glucose 6- phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway) [catalytic activity: trehalose 6-phosphate + H(2)O = trehalose + orthophosphate]; trehalose-6-phosphate phosphatase OtsB2 complement(965008..966180) Mycobacterium ulcerans Agy99 4551643 YP_905021.1 CDS dnaE2 NC_008611.1 967815 971081 D DNA polymerase involved in damage-induced mutagenesis and translesion synthesis. It is not the major replicative DNA polymerase.; error-prone DNA polymerase 967815..971081 Mycobacterium ulcerans Agy99 4551645 YP_905022.1 CDS MUL_0925 NC_008611.1 972061 973107 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 972061..973107 Mycobacterium ulcerans Agy99 4551648 YP_905023.1 CDS MUL_0926 NC_008611.1 973244 974167 R membrane protein; hypothetical protein complement(973244..974167) Mycobacterium ulcerans Agy99 4551649 YP_905024.1 CDS narK3_3 NC_008611.1 974498 976018 R membrane protein; involved in secretion of nitrite produced by the dissimilatory reduction of nitrate. responsible for the translocation of the substrate across the membrane.; nitrite extrusion protein, NarK3_3 complement(974498..976018) Mycobacterium ulcerans Agy99 4551650 YP_905025.1 CDS MUL_0928 NC_008611.1 975996 976418 R cytoplasmic protein; hypothetical protein complement(975996..976418) Mycobacterium ulcerans Agy99 4551651 YP_905026.1 CDS MUL_0929 NC_008611.1 976518 976730 R cytoplasmic protein; hypothetical protein complement(976518..976730) Mycobacterium ulcerans Agy99 4551652 YP_905027.1 CDS MUL_0931 NC_008611.1 977672 979270 R cytoplasmic protein; may be a regulatory protein; hypothetical protein complement(977672..979270) Mycobacterium ulcerans Agy99 4551654 YP_905028.1 CDS rocA NC_008611.1 979304 980986 D Detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; involved in the arginase pathway [catalytic activity: 1-pyrroline-5-carboxylate + NAD(+) + H(2)O = L- glutamate + NADH]; pyrroline-5-carboxylate dehydrogenase, RocA 979304..980986 Mycobacterium ulcerans Agy99 4551655 YP_905029.1 CDS putA NC_008611.1 980986 981945 D cytoplasmic protein; oxidizes proline to glutamate for use as a carbon and nitrogen source [catalytic activity: L-proline + acceptor + H2O = (S)-1-pyrroline-5-carboxylate + reduced acceptor]; proline dehydrogenase, PutA 980986..981945 Mycobacterium ulcerans Agy99 4552184 YP_905030.1 CDS MUL_0934 NC_008611.1 981972 982625 R cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulator complement(981972..982625) Mycobacterium ulcerans Agy99 4552185 YP_905031.1 CDS ltp2 NC_008611.1 982609 983778 R membrane protein; lipid-transfer protein; lipid-transfer protein complement(982609..983778) Mycobacterium ulcerans Agy99 4552186 YP_905032.1 CDS MUL_0936 NC_008611.1 983775 984230 R cytoplasmic protein; hypothetical protein complement(983775..984230) Mycobacterium ulcerans Agy99 4552187 YP_905033.1 CDS MUL_0937 NC_008611.1 984227 984655 R cytoplasmic protein; hypothetical protein complement(984227..984655) Mycobacterium ulcerans Agy99 4552188 YP_905034.1 CDS MUL_0938 NC_008611.1 984655 985320 R cytoplasmic protein; hypothetical protein complement(984655..985320) Mycobacterium ulcerans Agy99 4552189 YP_905035.1 CDS aldA_1 NC_008611.1 985483 986949 D cytoplasmic protein; oxidizes a variety of aldehydes [catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH]; NAD-dependent aldehyde dehydrogenase AldA 985483..986949 Mycobacterium ulcerans Agy99 4552190 YP_905036.1 CDS MUL_0942 NC_008611.1 988063 988866 D cytoplasmic protein; hypothetical protein 988063..988866 Mycobacterium ulcerans Agy99 4552192 YP_905037.1 CDS fadD36 NC_008611.1 988911 990329 D activates fatty acids by binding to coenzyme A; acyl-CoA synthetase 988911..990329 Mycobacterium ulcerans Agy99 4552193 YP_905038.1 CDS MUL_0944 NC_008611.1 990332 991597 R cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein complement(990332..991597) Mycobacterium ulcerans Agy99 4552194 YP_905039.1 CDS MUL_0946 NC_008611.1 992344 992643 D PE31; membrane protein; PPE family protein 992344..992643 Mycobacterium ulcerans Agy99 4552195 YP_905040.1 CDS MUL_0947 NC_008611.1 992683 993864 D PPE18; membrane protein; PPE family protein 992683..993864 Mycobacterium ulcerans Agy99 4552196 YP_905041.1 CDS MUL_0948 NC_008611.1 994021 994683 R hypothetical protein complement(994021..994683) Mycobacterium ulcerans Agy99 4552197 YP_905042.1 CDS MUL_0949 NC_008611.1 995097 996380 D membrane protein; integral membrane transport protein 995097..996380 Mycobacterium ulcerans Agy99 4552198 YP_905043.1 CDS MUL_0950 NC_008611.1 997038 998090 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 997038..998090 Mycobacterium ulcerans Agy99 4552199 YP_905044.1 CDS MUL_0951 NC_008611.1 998130 998492 R membrane protein; hypothetical protein complement(998130..998492) Mycobacterium ulcerans Agy99 4552200 YP_905045.1 CDS MUL_0952 NC_008611.1 998647 999147 D cytoplasmic protein; putative regulatory protein 998647..999147 Mycobacterium ulcerans Agy99 4552201 YP_905046.1 CDS MUL_0953 NC_008611.1 999202 1000146 R Detected in the membrane fraction by proteomics.; membrane protein; function unknown, probably involved in cellular metabolism; transferase complement(999202..1000146) Mycobacterium ulcerans Agy99 4552202 YP_905047.1 CDS dapE NC_008611.1 1000269 1001333 D catalyzes the formation of succinate and diaminoheptanedioate from succinyldiaminoheptanedioate; succinyl-diaminopimelate desuccinylase 1000269..1001333 Mycobacterium ulcerans Agy99 4552203 YP_905048.1 CDS MUL_0955 NC_008611.1 1001361 1003466 R membrane protein; hypothetical protein complement(1001361..1003466) Mycobacterium ulcerans Agy99 4552204 YP_905049.1 CDS MUL_0956 NC_008611.1 1003593 1004156 D cytoplasmic protein; hypothetical protein 1003593..1004156 Mycobacterium ulcerans Agy99 4552205 YP_905050.1 CDS fadD6 NC_008611.1 1004227 1006008 D activates fatty acids by binding to coenzyme A; may be involved in acyclic terpene utilization; long-chain-acyl-CoA synthetase 1004227..1006008 Mycobacterium ulcerans Agy99 4552206 YP_905051.1 CDS folP2 NC_008611.1 1006098 1006973 D involved in dihydrofolate biosynthesis [catalytic activity : 2-amino-4-hydroxy-6-hydroxymethyl-7,8- dihydropteridine diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate]; dihydropteroate synthase 2 FolP2 1006098..1006973 Mycobacterium ulcerans Agy99 4552207 YP_905052.1 CDS MUL_0959 NC_008611.1 1006970 1007953 D cytoplasmic protein; putative glucosyl-3-phosphoglycerate synthase 1006970..1007953 Mycobacterium ulcerans Agy99 4552208 YP_905053.1 CDS MUL_0960 NC_008611.1 1008002 1008373 D Detected in the membrane fraction by proteomics (LC- MS/MS); secreted protein; hypothetical protein 1008002..1008373 Mycobacterium ulcerans Agy99 4552209 YP_905054.1 CDS tagA NC_008611.1 1008370 1008948 D cytoplasmic protein; involved in base excision repair. hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions; DNA-3-methyladenine glycosylase I TagA 1008370..1008948 Mycobacterium ulcerans Agy99 4552210 YP_905055.1 CDS MUL_0962 NC_008611.1 1009053 1009280 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS; cytoplasmic protein; hypothetical protein 1009053..1009280 Mycobacterium ulcerans Agy99 4552211 YP_905056.1 CDS MUL_0964 NC_008611.1 1010063 1011928 D secreted protein; PPE family protein 1010063..1011928 Mycobacterium ulcerans Agy99 4552213 YP_905057.1 CDS MUL_0965 NC_008611.1 1012406 1015081 D secreted protein; PPE family protein 1012406..1015081 Mycobacterium ulcerans Agy99 4552214 YP_905058.1 CDS MUL_0966 NC_008611.1 1015138 1016472 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(1015138..1016472) Mycobacterium ulcerans Agy99 4552215 YP_905059.1 CDS MUL_0967 NC_008611.1 1016724 1018367 R secreted protein; PE family protein complement(1016724..1018367) Mycobacterium ulcerans Agy99 4552216 YP_905060.1 CDS fadA6_3 NC_008611.1 1022048 1023196 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); cytoplasmic protein; function unknown, but involved in lipid degradation [catalytic activity: 2 acetyl-CoA = CoA + acetoacetyl- CoA]; acetyl-CoA acetyltransferase 1022048..1023196 Mycobacterium ulcerans Agy99 4552219 YP_905061.1 CDS echA1_1 NC_008611.1 1023193 1023996 D cytoplasmic protein; oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]; enoyl-CoA hydratase, EchA1 1023193..1023996 Mycobacterium ulcerans Agy99 4552794 YP_905062.1 CDS MUL_0974 NC_008611.1 1026444 1027778 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(1026444..1027778) Mycobacterium ulcerans Agy99 4552796 YP_905063.1 CDS MUL_0976 NC_008611.1 1028294 1029340 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(1028294..1029340) Mycobacterium ulcerans Agy99 4552798 YP_905064.1 CDS MUL_0977 NC_008611.1 1029718 1031052 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 1029718..1031052 Mycobacterium ulcerans Agy99 4552799 YP_905065.1 CDS MUL_0979 NC_008611.1 1032499 1033767 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase complement(1032499..1033767) Mycobacterium ulcerans Agy99 4552801 YP_905066.1 CDS MUL_0980 NC_008611.1 1033764 1034273 R membrane protein; hypothetical protein complement(1033764..1034273) Mycobacterium ulcerans Agy99 4552802 YP_905067.1 CDS MUL_0982 NC_008611.1 1035419 1036522 D cytoplasmic protein; hypothetical protein 1035419..1036522 Mycobacterium ulcerans Agy99 4552804 YP_905068.1 CDS MUL_0983 NC_008611.1 1036586 1037395 D membrane protein; integral membrane protein 1036586..1037395 Mycobacterium ulcerans Agy99 4552805 YP_905069.1 CDS echA10 NC_008611.1 1038864 1039667 R Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase complement(1038864..1039667) Mycobacterium ulcerans Agy99 4552807 YP_905070.1 CDS mcr NC_008611.1 1039821 1040918 D cytoplasmic protein; required for bile acid synthesis and for catabolism of branched-chain fatty acids; alpha-methylacyl-CoA racemase Mcr 1039821..1040918 Mycobacterium ulcerans Agy99 4552808 YP_905071.1 CDS MUL_0987 NC_008611.1 1040976 1041737 D Detected in the cytoplasmic and membrane fraction by LC-MS/MS. Also detected in the extracellular matrix by proteomics.; cytoplasmic protein; function unknown, supposed involved in cellular metabolism.; short-chain type dehydrogenase/reductase 1040976..1041737 Mycobacterium ulcerans Agy99 4552809 YP_905072.1 CDS MUL_0988 NC_008611.1 1041874 1042029 R hypothetical protein complement(1041874..1042029) Mycobacterium ulcerans Agy99 4552810 YP_905073.1 CDS mmpL13 NC_008611.1 1042387 1044729 D membrane protein; thought to be involved in fatty acid transport.; transmembrane transport protein MmpL13 1042387..1044729 Mycobacterium ulcerans Agy99 4552811 YP_905074.1 CDS MUL_0990 NC_008611.1 1044746 1045429 D cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator 1044746..1045429 Mycobacterium ulcerans Agy99 4552812 YP_905075.1 CDS MUL_0991 NC_008611.1 1045426 1046064 D cytoplasmic protein; hypothetical protein 1045426..1046064 Mycobacterium ulcerans Agy99 4552813 YP_905076.1 CDS MUL_0992 NC_008611.1 1046172 1046888 R Modulates the activities of several enzymes which are inactive in their acetylated form; NAD-dependent deacetylase complement(1046172..1046888) Mycobacterium ulcerans Agy99 4552814 YP_905077.1 CDS MUL_0993 NC_008611.1 1046947 1047312 D cytoplasmic protein; involved in transcriptional mechanism; transcriptional regulatory protein 1046947..1047312 Mycobacterium ulcerans Agy99 4552815 YP_905078.1 CDS MUL_0995 NC_008611.1 1048174 1048725 R cytoplasmic protein; hypothetical protein complement(1048174..1048725) Mycobacterium ulcerans Agy99 4552817 YP_905079.1 CDS MUL_0996 NC_008611.1 1048746 1049189 D Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); cytoplasmic protein; hypothetical protein 1048746..1049189 Mycobacterium ulcerans Agy99 4552818 YP_905080.1 CDS MUL_0997 NC_008611.1 1049212 1049400 D cytoplasmic protein; hypothetical protein 1049212..1049400 Mycobacterium ulcerans Agy99 4552819 YP_905081.1 CDS MUL_0998 NC_008611.1 1050322 1050519 D cytoplasmic protein; hypothetical protein 1050322..1050519 Mycobacterium ulcerans Agy99 4552820 YP_905082.1 CDS MUL_0999 NC_008611.1 1050554 1050958 R membrane protein; hypothetical protein complement(1050554..1050958) Mycobacterium ulcerans Agy99 4552821 YP_905083.1 CDS MUL_1000 NC_008611.1 1051011 1052057 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(1051011..1052057) Mycobacterium ulcerans Agy99 4552822 YP_905084.1 CDS MUL_1001 NC_008611.1 1052616 1053740 R membrane protein; hypothetical protein complement(1052616..1053740) Mycobacterium ulcerans Agy99 4552823 YP_905085.1 CDS MUL_1002 NC_008611.1 1053998 1054573 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; hypothetical protein 1053998..1054573 Mycobacterium ulcerans Agy99 4552824 YP_905086.1 CDS MUL_1003 NC_008611.1 1054598 1054744 D cytoplasmic protein; hypothetical protein 1054598..1054744 Mycobacterium ulcerans Agy99 4552825 YP_905087.1 CDS MUL_1004 NC_008611.1 1054776 1055987 R secreted protein; hypothetical protein complement(1054776..1055987) Mycobacterium ulcerans Agy99 4552826 YP_905088.1 CDS MUL_1005 NC_008611.1 1056037 1056663 R secreted protein; hypothetical protein complement(1056037..1056663) Mycobacterium ulcerans Agy99 4552827 YP_905089.1 CDS pimE NC_008611.1 1056805 1058073 D involved in the fifth mannose transfer of phosphatidylinositol mannoside synthesis; mannosyltransferase 1056805..1058073 Mycobacterium ulcerans Agy99 4552828 YP_905090.1 CDS phhB NC_008611.1 1058099 1058377 R 4-alpha-hydroxytetrahydrobiopterin dehydratase activity; catalyzes the formation of (6R)-6-(L-erythro-1,2-dihydroxypropyl)-7, 8-dihydro-6H-pterin from (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7, 8-tetrahydro-4a-hydroxypterin; functions in recycling tetrahydrobiopterin (BH4) in phenylalanine hydroxylase reaction; pterin-4-alpha-carbinolamine dehydratase complement(1058099..1058377) Mycobacterium ulcerans Agy99 4552829 YP_905091.1 CDS mutT2 NC_008611.1 1058637 1059059 D cytoplasmic protein; involved in the go system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8- oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite da and dc residues of template DNA with almost equal efficiency thus leading to AT to GC transversions. MutT specifically degrades 8-oxo-dGTP to the monophosphate.; mutator protein MutT2 1058637..1059059 Mycobacterium ulcerans Agy99 4552830 YP_905092.1 CDS typA NC_008611.1 1059311 1061197 D cytoplasmic protein; function unknown, probably interacts with the ribosomes in a GTP dependent manner; GTP-binding translation elongation factor TypA 1059311..1061197 Mycobacterium ulcerans Agy99 4552831 YP_905093.1 CDS lpqW NC_008611.1 1061301 1063199 D membrane protein; lipoprotein LpqW 1061301..1063199 Mycobacterium ulcerans Agy99 4552832 YP_905094.1 CDS MUL_1011 NC_008611.1 1063504 1064550 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 1063504..1064550 Mycobacterium ulcerans Agy99 4552833 YP_905095.1 CDS MUL_1012 NC_008611.1 1064784 1066115 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 1064784..1066115 Mycobacterium ulcerans Agy99 4552834 YP_905096.1 CDS mshB NC_008611.1 1070568 1071473 D cytoplasmic protein; involved in mycothiol biosynthesis. 1-D-myo- inosityl-2-acetamido-2-deoxy-alpha-D-glucopyranoside (GlcNAc-Ins)is converted to 1-D-myo-inosityl-2-amino-2- deoxy-alpha-D-glucopyranoside (GlcN-Ins) by this enzyme. seems to possesse weak mycothiol conjugate ami; N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha- D-glucopyranoside deacetylase MshB 1070568..1071473 Mycobacterium ulcerans Agy99 4552838 YP_905097.1 CDS MUL_1018 NC_008611.1 1071684 1072043 D membrane protein; function unknown -possibly a transmembrane protein; hypothetical protein 1071684..1072043 Mycobacterium ulcerans Agy99 4552839 YP_905098.1 CDS MUL_1019 NC_008611.1 1072073 1072960 R secreted protein; PE family protein complement(1072073..1072960) Mycobacterium ulcerans Agy99 4552840 YP_905099.1 CDS fbiC NC_008611.1 1073165 1075735 D 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase; catalyzes radical-mediated transfer of hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO); functions in F420 biosynthesis; FO synthase 1073165..1075735 Mycobacterium ulcerans Agy99 4552841 YP_905100.1 CDS MUL_1021 NC_008611.1 1076307 1078850 D membrane protein; PE-PGRS family protein 1076307..1078850 Mycobacterium ulcerans Agy99 4552842 YP_905101.1 CDS MUL_1022 NC_008611.1 1079060 1079395 R secreted protein; function unknown function (secreted protein); putative antigen complement(1079060..1079395) Mycobacterium ulcerans Agy99 4552692 YP_905102.1 CDS fadH NC_008611.1 1079607 1081631 R cytoplasmic protein; catalyzes the NADP-dependent reduction of 2,4- dienoyl-CoA to yield trans-2- enoyl-CoA [catalytic activity: trans-2,3-didehydroacyl-CoA + NADP(+) = trans, trans-2,3,4,5-tetradehydroacyl-CoA + NADPH]; NADPH dependent 2,4-dienoyl-CoA reductase FadH complement(1079607..1081631) Mycobacterium ulcerans Agy99 4552693 YP_905103.1 CDS MUL_1024 NC_008611.1 1081631 1082374 R cytoplasmic protein; hypothetical protein complement(1081631..1082374) Mycobacterium ulcerans Agy99 4552694 YP_905104.1 CDS fdxC NC_008611.1 1082411 1082737 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.; ferredoxin FdxC 1082411..1082737 Mycobacterium ulcerans Agy99 4552695 YP_905105.1 CDS MUL_1026 NC_008611.1 1082767 1083864 D catalyzes the formation of N-succinyl-LL-2,6-diaminopimelate from N-succinyl-L-2-amino-6-oxopimelate in lysine biosynthesis; N-succinyldiaminopimelate aminotransferase 1082767..1083864 Mycobacterium ulcerans Agy99 4552696 YP_905106.1 CDS MUL_1030 NC_008611.1 1089450 1090784 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 1089450..1090784 Mycobacterium ulcerans Agy99 4552699 YP_905107.1 CDS MUL_1034 NC_008611.1 1093210 1094727 R PE4; membrane protein; PE family protein complement(1093210..1094727) Mycobacterium ulcerans Agy99 4552703 YP_905108.1 CDS MUL_1035 NC_008611.1 1094855 1095775 R cytoplasmic protein; hypothetical protein complement(1094855..1095775) Mycobacterium ulcerans Agy99 4552704 YP_905109.1 CDS fabG3 NC_008611.1 1096982 1097722 D cytoplasmic protein; not really known; thought to be involved in lipid biosynthesis [catalytic activity: androstan-3-alpha,17- beta-diol + NAD+ = 17-beta-hydroxyandrostan-3-one + NADH]; 20-beta-hydroxysteroid dehydrogenase FabG3 1096982..1097722 Mycobacterium ulcerans Agy99 4552706 YP_905110.1 CDS MUL_1038 NC_008611.1 1097743 1099083 D membrane protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 1097743..1099083 Mycobacterium ulcerans Agy99 4552707 YP_905111.1 CDS MUL_1039 NC_008611.1 1099090 1099635 R cytoplasmic protein; function unknown. contains TetR_N, bacterial regulatory proteins, TetR family domain. possibly involved in transcriptional mechanism.; putative regulatory protein complement(1099090..1099635) Mycobacterium ulcerans Agy99 4552708 YP_905112.1 CDS MUL_1043 NC_008611.1 1102416 1103312 D cytoplasmic protein; hypothetical protein 1102416..1103312 Mycobacterium ulcerans Agy99 4553194 YP_905113.1 CDS MUL_1044 NC_008611.1 1103335 1104012 R cytoplasmic protein; hypothetical protein complement(1103335..1104012) Mycobacterium ulcerans Agy99 4553195 YP_905114.1 CDS MUL_1045 NC_008611.1 1103877 1105163 R cytoplasmic protein; hypothetical protein complement(1103877..1105163) Mycobacterium ulcerans Agy99 4553196 YP_905115.1 CDS MUL_1046 NC_008611.1 1105280 1106449 D cytoplasmic protein; acyl-CoA transferase 1105280..1106449 Mycobacterium ulcerans Agy99 4553197 YP_905116.1 CDS MUL_1047 NC_008611.1 1106691 1107059 D cytoplasmic protein; hypothetical protein 1106691..1107059 Mycobacterium ulcerans Agy99 4553198 YP_905117.1 CDS cyp191A3 NC_008611.1 1107880 1109088 D cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.; cytochrome P450 191A3 Cyp191A3 1107880..1109088 Mycobacterium ulcerans Agy99 4553200 YP_905118.1 CDS MUL_1051 NC_008611.1 1110055 1110816 D cytoplasmic protein; hypothetical protein 1110055..1110816 Mycobacterium ulcerans Agy99 4553202 YP_905119.1 CDS MUL_1052 NC_008611.1 1110813 1111547 D cytoplasmic protein; short chain dehydrogenase 1110813..1111547 Mycobacterium ulcerans Agy99 4553203 YP_905120.1 CDS MUL_1053 NC_008611.1 1111544 1113136 D membrane protein; function unknown, probably involved in cellular metabolism.; monooxygenase 1111544..1113136 Mycobacterium ulcerans Agy99 4553204 YP_905121.1 CDS lipW NC_008611.1 1113151 1113987 D cytoplasmic protein; function unknown, lipolytic enzyme probably involved in cellular metabolism.; esterase LipW 1113151..1113987 Mycobacterium ulcerans Agy99 4553205 YP_905122.1 CDS MUL_1056 NC_008611.1 1115138 1115611 D hypothetical protein 1115138..1115611 Mycobacterium ulcerans Agy99 4553207 YP_905123.1 CDS MUL_1057 NC_008611.1 1115663 1116124 D Also detected in the membrane fraction by proteomics (LC-MS/MS); cytoplasmic protein; hypothetical protein 1115663..1116124 Mycobacterium ulcerans Agy99 4553208 YP_905124.1 CDS MUL_1058 NC_008611.1 1116149 1116841 R cytoplasmic protein; possibly involved in transcriptional mechanism.; GntR family transcriptional regulator complement(1116149..1116841) Mycobacterium ulcerans Agy99 4553209 YP_905125.1 CDS yrbE1A NC_008611.1 1118876 1119679 D membrane protein; integral membrane protein YrbE1A 1118876..1119679 Mycobacterium ulcerans Agy99 4553211 YP_905126.1 CDS yrbE1B NC_008611.1 1119681 1120550 D membrane protein; integral membrane protein YrbE1B 1119681..1120550 Mycobacterium ulcerans Agy99 4553212 YP_905127.1 CDS mce1A NC_008611.1 1120555 1121859 D membrane protein; function unknown, but thought to be involved in host cell invasion (entry and survival inside macrophages); MCE-family protein Mce1A 1120555..1121859 Mycobacterium ulcerans Agy99 4553213 YP_905128.1 CDS mce1B NC_008611.1 1121856 1122896 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE-family protein Mce1B 1121856..1122896 Mycobacterium ulcerans Agy99 4553214 YP_905129.1 CDS mce1C NC_008611.1 1122893 1124458 D membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE-family protein Mce1C 1122893..1124458 Mycobacterium ulcerans Agy99 4553215 YP_905130.1 CDS mce1D NC_008611.1 1124455 1126062 D membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE-family protein Mce1D 1124455..1126062 Mycobacterium ulcerans Agy99 4553216 YP_905131.1 CDS lprK NC_008611.1 1126080 1127285 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE family lipoprotein LprK 1126080..1127285 Mycobacterium ulcerans Agy99 4553217 YP_905132.1 CDS mce1F NC_008611.1 1127279 1128832 D Also detected in the membrane fraction by proteomics.; membrane protein; function unknown, but thought involved in host cell invasion.; MCE-family protein Mce1F 1127279..1128832 Mycobacterium ulcerans Agy99 4553218 YP_905133.1 CDS MUL_1068 NC_008611.1 1128856 1129530 D Detected in the membrane fraction by proteomics (LC- MS/MS) Also detected in the cytoplasmic fraction (2D- MALDI); membrane protein; Mce associated membrane protein 1128856..1129530 Mycobacterium ulcerans Agy99 4553219 YP_905134.1 CDS MUL_1069 NC_008611.1 1129527 1130516 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; Mce associated transmembrane protein 1129527..1130516 Mycobacterium ulcerans Agy99 4553220 YP_905135.1 CDS MUL_1070 NC_008611.1 1130513 1131070 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; Mce associated protein 1130513..1131070 Mycobacterium ulcerans Agy99 4553221 YP_905136.1 CDS MUL_1071 NC_008611.1 1131037 1131831 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; Mce associated membrane protein 1131037..1131831 Mycobacterium ulcerans Agy99 4553222 YP_905137.1 CDS MUL_1073 NC_008611.1 1133090 1134463 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; transmembrane protein complement(1133090..1134463) Mycobacterium ulcerans Agy99 4553224 YP_905138.1 CDS MUL_1075 NC_008611.1 1135440 1136873 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; hypothetical protein complement(1135440..1136873) Mycobacterium ulcerans Agy99 4553226 YP_905139.1 CDS sigG NC_008611.1 1136870 1137931 R DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates; RNA polymerase factor sigma-70 complement(1136870..1137931) Mycobacterium ulcerans Agy99 4553227 YP_905140.1 CDS MUL_1077 NC_008611.1 1137963 1138802 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; lysophospholipase 1137963..1138802 Mycobacterium ulcerans Agy99 4553228 YP_905141.1 CDS MUL_1078 NC_008611.1 1138849 1139598 D cytoplasmic protein; hypothetical protein 1138849..1139598 Mycobacterium ulcerans Agy99 4553229 YP_905142.1 CDS MUL_1079 NC_008611.1 1139595 1140119 D cytoplasmic protein; function unknown, probably involved in a cellular metabolism.; hypothetical protein 1139595..1140119 Mycobacterium ulcerans Agy99 4553230 YP_905143.1 CDS bglS NC_008611.1 1140176 1142251 D membrane protein; possibly involved in degradation [catalytic activity: hydrolysis of terminal, non-reducing beta-D- glucose residues with release of beta-D-glucose]; beta-glucosidase BglS 1140176..1142251 Mycobacterium ulcerans Agy99 4553231 YP_905144.1 CDS ilvD NC_008611.1 1145497 1147191 R catalyzes the dehydration of 2,3-dihydroxy-3-methylbutanoate to 3-methyl-2-oxobutanoate in valine and isoleucine biosynthesis; dihydroxy-acid dehydratase complement(1145497..1147191) Mycobacterium ulcerans Agy99 4553233 YP_905145.1 CDS MUL_1083 NC_008611.1 1147331 1147621 D cytoplasmic protein; hypothetical protein 1147331..1147621 Mycobacterium ulcerans Agy99 4553234 YP_905146.1 CDS MUL_1084 NC_008611.1 1147739 1148995 D membrane protein; function unknown, possibly involved in transport of drug across the membrane.; integral membrane protein 1147739..1148995 Mycobacterium ulcerans Agy99 4553235 YP_905147.1 CDS MUL_1085 NC_008611.1 1149115 1150113 D secreted protein; secretory protein 1149115..1150113 Mycobacterium ulcerans Agy99 4553236 YP_905148.1 CDS MUL_1086 NC_008611.1 1150332 1150907 D cytoplasmic protein; possibly involved in transcriptional mechanism.; transcriptional regulatory protein 1150332..1150907 Mycobacterium ulcerans Agy99 4553237 YP_905149.1 CDS MUL_1088 NC_008611.1 1153187 1155181 R cytoplasmic protein; function unknown, hydrolyzes peptides and/or proteins.; zinc metalloprotease complement(1153187..1155181) Mycobacterium ulcerans Agy99 4553239 YP_905150.1 CDS MUL_1089 NC_008611.1 1155224 1155871 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 1155224..1155871 Mycobacterium ulcerans Agy99 4553240 YP_905151.1 CDS MUL_1090 NC_008611.1 1155868 1156557 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; transmembrane protein 1155868..1156557 Mycobacterium ulcerans Agy99 4553241 YP_905152.1 CDS MUL_1091 NC_008611.1 1156544 1157083 R cytoplasmic protein; hypothetical protein complement(1156544..1157083) Mycobacterium ulcerans Agy99 4553242 YP_905153.1 CDS mmpL11 NC_008611.1 1157080 1160097 R membrane protein; function unknown. thought to be involved in fatty acid transport.; transmembrane transport protein MmpL11 complement(1157080..1160097) Mycobacterium ulcerans Agy99 4553243 YP_905154.1 CDS MUL_1093 NC_008611.1 1160452 1160961 D secreted protein; exported protein 1160452..1160961 Mycobacterium ulcerans Agy99 4553244 YP_905155.1 CDS MUL_1094 NC_008611.1 1160963 1162180 R membrane protein; transmembrane protein complement(1160963..1162180) Mycobacterium ulcerans Agy99 4553514 YP_905156.1 CDS mmpL3 NC_008611.1 1163592 1166474 R Detected in the membrane fraction by proteomics (LC- MS/MS) Also detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; function unknown. thought to be involved in fatty acid transport.; transmembrane transport protein MmpL3 complement(1163592..1166474) Mycobacterium ulcerans Agy99 4553516 YP_905157.1 CDS MUL_1097 NC_008611.1 1166580 1167317 R cytoplasmic protein; hypothetical protein complement(1166580..1167317) Mycobacterium ulcerans Agy99 4553517 YP_905158.1 CDS trmB NC_008611.1 1167355 1168191 R tRNA (guanine-N(7)-)-methyltransferase; catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA by transferring the methyl residue from S-adenosyl-L-methionine; tRNA (guanine-N(7)-)-methyltransferase complement(1167355..1168191) Mycobacterium ulcerans Agy99 4553518 YP_905159.1 CDS MUL_1099 NC_008611.1 1168355 1169485 D cytoplasmic protein; hypothetical protein 1168355..1169485 Mycobacterium ulcerans Agy99 4553519 YP_905160.1 CDS MUL_1100 NC_008611.1 1169383 1171035 D Also detected in the cytoplasmic fraction by 2D-LC- MS/MS.; secreted protein; hypothetical protein 1169383..1171035 Mycobacterium ulcerans Agy99 4553520 YP_905161.1 CDS pckA NC_008611.1 1171238 1173067 D catalyzes the phosphorylation and decarboxylation of oxaloacetate to form phosphoenolpyruvate using GTP; phosphoenolpyruvate carboxykinase 1171238..1173067 Mycobacterium ulcerans Agy99 4553521 YP_905162.1 CDS echA8_4 NC_008611.1 1174603 1175571 D cytoplasmic protein; oxidizes fatty acids using specific components (by similarity) [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]; enoyl-CoA hydratase, EchA8_4 1174603..1175571 Mycobacterium ulcerans Agy99 4553523 YP_905163.1 CDS MUL_1104 NC_008611.1 1175568 1175825 R membrane protein; hypothetical protein complement(1175568..1175825) Mycobacterium ulcerans Agy99 4553524 YP_905164.1 CDS MUL_1106 NC_008611.1 1177424 1178470 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 1177424..1178470 Mycobacterium ulcerans Agy99 4553526 YP_905165.1 CDS MUL_1107 NC_008611.1 1178681 1179688 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; hypothetical protein 1178681..1179688 Mycobacterium ulcerans Agy99 4553527 YP_905166.1 CDS MUL_1109 NC_008611.1 1180974 1182245 D cytoplasmic protein; hypothetical protein 1180974..1182245 Mycobacterium ulcerans Agy99 4553529 YP_905167.1 CDS MUL_1110 NC_008611.1 1183273 1184619 D membrane protein; hypothetical protein 1183273..1184619 Mycobacterium ulcerans Agy99 4553531 YP_905168.1 CDS MUL_1111 NC_008611.1 1184621 1185139 D membrane protein; hypothetical protein 1184621..1185139 Mycobacterium ulcerans Agy99 4553532 YP_905169.1 CDS lipC NC_008611.1 1185295 1186473 D cytoplasmic protein; function unknown, lipolytic enzyme probably involved in cellular metabolism.; esterase LipC 1185295..1186473 Mycobacterium ulcerans Agy99 4553533 YP_905170.1 CDS echA1 NC_008611.1 1187955 1188737 D Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase 1187955..1188737 Mycobacterium ulcerans Agy99 4553535 YP_905171.1 CDS MUL_1115 NC_008611.1 1188836 1190323 D cytoplasmic protein; function unknown, but involved in lipid degradation.; long-chain-fatty-acid-CoA ligase 1188836..1190323 Mycobacterium ulcerans Agy99 4553536 YP_905172.1 CDS MUL_1116 NC_008611.1 1190353 1190616 D cytoplasmic protein; hypothetical protein 1190353..1190616 Mycobacterium ulcerans Agy99 4553537 YP_905173.1 CDS MUL_1117 NC_008611.1 1191193 1192920 D membrane protein; function unknown, but involvement in lipid degradation.; fatty-acid-CoA ligase 1191193..1192920 Mycobacterium ulcerans Agy99 4553538 YP_905174.1 CDS MUL_1119 NC_008611.1 1197774 1198394 R membrane protein; possibly involved in transcriptional mechanism.; transcriptional regulatory protein complement(1197774..1198394) Mycobacterium ulcerans Agy99 4553540 YP_905175.1 CDS MUL_1121 NC_008611.1 1198750 1199520 D Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase 1198750..1199520 Mycobacterium ulcerans Agy99 4553541 YP_905176.1 CDS MUL_1122 NC_008611.1 1199585 1201051 R membrane protein; thought to oxidize a wide variety of aliphatic and aromatic aldehydes.; aldehyde dehydrogenase complement(1199585..1201051) Mycobacterium ulcerans Agy99 4553542 YP_905177.1 CDS MUL_1123 NC_008611.1 1201096 1201863 R membrane protein; causes methylation; methyltransferase complement(1201096..1201863) Mycobacterium ulcerans Agy99 4553543 YP_905178.1 CDS MUL_1124 NC_008611.1 1201919 1203082 D cytoplasmic protein; function unknown, contains a glycosyltransferase domain (cell envelope biogenesis, outer membrane); glycosyltransferase 1201919..1203082 Mycobacterium ulcerans Agy99 4553544 YP_905179.1 CDS MUL_1125 NC_008611.1 1203092 1204879 R membrane protein; transmembrane protein complement(1203092..1204879) Mycobacterium ulcerans Agy99 4553545 YP_905180.1 CDS MUL_1126 NC_008611.1 1204902 1206167 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(1204902..1206167) Mycobacterium ulcerans Agy99 4553546 YP_905181.1 CDS MUL_1127 NC_008611.1 1206320 1207528 D membrane protein; function unknown, probably involved in cellular metabolism.; integral membrane acyltransferase 1206320..1207528 Mycobacterium ulcerans Agy99 4553547 YP_905182.1 CDS MUL_1129 NC_008611.1 1208362 1209408 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 1208362..1209408 Mycobacterium ulcerans Agy99 4553549 YP_905183.1 CDS MUL_1130 NC_008611.1 1210925 1211221 R cytoplasmic protein; hypothetical protein complement(1210925..1211221) Mycobacterium ulcerans Agy99 4553451 YP_905184.1 CDS MUL_1131 NC_008611.1 1211160 1211516 R cytoplasmic protein; hypothetical protein complement(1211160..1211516) Mycobacterium ulcerans Agy99 4553452 YP_905185.1 CDS MUL_1132 NC_008611.1 1211867 1212136 R cytoplasmic protein; hypothetical protein complement(1211867..1212136) Mycobacterium ulcerans Agy99 4553453 YP_905186.1 CDS MUL_1133 NC_008611.1 1212121 1213065 R cytoplasmic protein; hypothetical protein complement(1212121..1213065) Mycobacterium ulcerans Agy99 4553454 YP_905187.1 CDS MUL_1134 NC_008611.1 1213239 1213526 R cytoplasmic protein; hypothetical protein complement(1213239..1213526) Mycobacterium ulcerans Agy99 4553455 YP_905188.1 CDS MUL_1135 NC_008611.1 1214094 1214321 D cytoplasmic protein; hypothetical protein 1214094..1214321 Mycobacterium ulcerans Agy99 4553456 YP_905189.1 CDS MUL_1136 NC_008611.1 1214390 1215136 R membrane protein; hypothetical protein complement(1214390..1215136) Mycobacterium ulcerans Agy99 4553457 YP_905190.1 CDS MUL_1138 NC_008611.1 1215861 1216907 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(1215861..1216907) Mycobacterium ulcerans Agy99 4553459 YP_905191.1 CDS MUL_1140 NC_008611.1 1218243 1218656 D membrane protein; hypothetical protein 1218243..1218656 Mycobacterium ulcerans Agy99 4553461 YP_905192.1 CDS MUL_1141 NC_008611.1 1218666 1218959 D membrane protein; hypothetical protein 1218666..1218959 Mycobacterium ulcerans Agy99 4553462 YP_905193.1 CDS MUL_1142 NC_008611.1 1219033 1219251 D Detected in the cytoplasmic and secreted fractions by 2D-LC-MS/MS.; secreted protein; hypothetical protein 1219033..1219251 Mycobacterium ulcerans Agy99 4553463 YP_905194.1 CDS MUL_1143 NC_008611.1 1219302 1220429 R cytoplasmic protein; hypothetical protein complement(1219302..1220429) Mycobacterium ulcerans Agy99 4553464 YP_905195.1 CDS MUL_1144 NC_008611.1 1220579 1221064 R membrane protein; hypothetical protein complement(1220579..1221064) Mycobacterium ulcerans Agy99 4554016 YP_905196.1 CDS MUL_1145 NC_008611.1 1221305 1223809 D PE_PGRS30; secreted protein; PE-PGRS family protein 1221305..1223809 Mycobacterium ulcerans Agy99 4554017 YP_905197.1 CDS MUL_1147 NC_008611.1 1224435 1225769 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(1224435..1225769) Mycobacterium ulcerans Agy99 4554019 YP_905198.1 CDS MUL_1148 NC_008611.1 1226060 1227562 D cytoplasmic protein; DNA polymerase 1226060..1227562 Mycobacterium ulcerans Agy99 4554020 YP_905199.1 CDS MUL_1149 NC_008611.1 1227605 1228489 R secreted protein; PE-PGRS family protein complement(1227605..1228489) Mycobacterium ulcerans Agy99 4554021 YP_905200.1 CDS php NC_008611.1 1228670 1229650 R cytoplasmic protein; enzymatic activity is not yet known [catalytic activity: aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol]; phosphotriesterase PHP complement(1228670..1229650) Mycobacterium ulcerans Agy99 4554022 YP_905201.1 CDS fadE4 NC_008611.1 1229992 1231698 D function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE4 1229992..1231698 Mycobacterium ulcerans Agy99 4554023 YP_905202.1 CDS MUL_1152 NC_008611.1 1231797 1232468 D cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator 1231797..1232468 Mycobacterium ulcerans Agy99 4554024 YP_905203.1 CDS gabD1 NC_008611.1 1232590 1233966 R NADP-dependent semialdehyde dehydrogenase; part of alternative pathway from alpha-ketoglutarate to succinate; succinate-semialdehyde dehydrogenase complement(1232590..1233966) Mycobacterium ulcerans Agy99 4554025 YP_905204.1 CDS MUL_1154 NC_008611.1 1234414 1235598 D membrane protein; PPE family protein 1234414..1235598 Mycobacterium ulcerans Agy99 4554026 YP_905205.1 CDS MUL_1155 NC_008611.1 1235788 1238157 D membrane protein; PE-PGRS family protein 1235788..1238157 Mycobacterium ulcerans Agy99 4554027 YP_905206.1 CDS MUL_1156 NC_008611.1 1238239 1239669 R membrane protein; hypothetical protein complement(1238239..1239669) Mycobacterium ulcerans Agy99 4554028 YP_905207.1 CDS glpQ2 NC_008611.1 1239794 1240555 R cytoplasmic protein; glycerophosphoryl diester phosphodiesterase hydrolyzes deacylated phospholipids to G3P and the corresponding alcohols [catalytic activity: a glycerophosphodiester + H(2)O = an alcohol + SN-glycerol 3- phosphate]; glycerophosphoryl diester phosphodiesterase complement(1239794..1240555) Mycobacterium ulcerans Agy99 4554029 YP_905208.1 CDS MUL_1158 NC_008611.1 1240719 1241279 D secreted protein; hypothetical protein 1240719..1241279 Mycobacterium ulcerans Agy99 4554030 YP_905209.1 CDS MUL_1159 NC_008611.1 1241283 1245461 R membrane protein; transmembrane protein complement(1241283..1245461) Mycobacterium ulcerans Agy99 4554031 YP_905210.1 CDS MUL_1160 NC_008611.1 1245550 1245723 R Detected in the membrane fraction by proteomics (LC- MS/MS); secreted protein; small secreted protein complement(1245550..1245723) Mycobacterium ulcerans Agy99 4554032 YP_905211.1 CDS MUL_1161 NC_008611.1 1245792 1246049 R secreted protein; putative small secreted protein complement(1245792..1246049) Mycobacterium ulcerans Agy99 4554033 YP_905212.1 CDS lpqI NC_008611.1 1246153 1247346 D secreted protein; lipoprotein LpqI 1246153..1247346 Mycobacterium ulcerans Agy99 4554034 YP_905213.1 CDS MUL_1163 NC_008611.1 1247433 1248059 D cytoplasmic protein; possibly involved in transcriptional mechanism.; TetR family transcriptional regulator 1247433..1248059 Mycobacterium ulcerans Agy99 4554035 YP_905214.1 CDS MUL_1164 NC_008611.1 1248078 1249373 D membrane protein; function unknown, possibly ion channel involved in transport of chloride across the membrane.; transmembrane protein 1248078..1249373 Mycobacterium ulcerans Agy99 4554036 YP_905215.1 CDS MUL_1165 NC_008611.1 1249385 1250236 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS) Also detected in the cytoplasmic fraction.; membrane protein; hypothetical protein complement(1249385..1250236) Mycobacterium ulcerans Agy99 4554037 YP_905216.1 CDS fabG NC_008611.1 1250255 1251619 R Catalyzes the first of the two reduction steps in the elongation cycle of fatty acid synthesis; 3-ketoacyl-ACP reductase complement(1250255..1251619) Mycobacterium ulcerans Agy99 4554038 YP_905217.1 CDS fadA2 NC_008611.1 1251789 1253114 D Catalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation; acetyl-CoA acetyltransferase 1251789..1253114 Mycobacterium ulcerans Agy99 4554039 YP_905218.1 CDS fadE5 NC_008611.1 1253399 1255264 R Detected in the cytoplasmic fraction by proteomics.; cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE5 complement(1253399..1255264) Mycobacterium ulcerans Agy99 4554040 YP_905219.1 CDS MUL_1169 NC_008611.1 1255777 1256265 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 1255777..1256265 Mycobacterium ulcerans Agy99 4554041 YP_905220.1 CDS MUL_5125 NC_008611.1 1257341 1257499 R cytoplasmic protein; hypothetical protein complement(1257341..1257499) Mycobacterium ulcerans Agy99 4554043 YP_905221.1 CDS sdhB_1 NC_008611.1 1257547 1258293 R catalyzes the fumarate and succinate interconversion; fumarate reductase is used under anaerobic conditions with glucose or glycerol as carbon source; fumarate reductase iron-sulfur subunit complement(1257547..1258293) Mycobacterium ulcerans Agy99 4554044 YP_905222.1 CDS sdhA NC_008611.1 1258293 1260221 R part of four member succinate dehydrogenase enzyme complex that forms a trimeric complex (trimer of tetramers); SdhA/B are the catalytic subcomplex and can exhibit succinate dehydrogenase activity in the absence of SdhC/D which are the membrane components and form cytochrome b556; SdhC binds ubiquinone; oxidizes succinate to fumarate while reducing ubiquinone to ubiquinol; succinate dehydrogenase flavoprotein subunit complement(1258293..1260221) Mycobacterium ulcerans Agy99 4554045 YP_905223.1 CDS MUL_1173 NC_008611.1 1260268 1261089 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; could be involved in interconversion of fumarate and succinate (aerobic respiration) this hydrophobic component may be required to anchor the catalytic components of the succinate dehydrogenase complex to the cytoplasmic membrane.; succinate dehydrogenase (membrane anchor subunit) complement(1260268..1261089) Mycobacterium ulcerans Agy99 4554046 YP_905224.1 CDS MUL_1175 NC_008611.1 1261636 1262340 D cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 1261636..1262340 Mycobacterium ulcerans Agy99 4554048 YP_905225.1 CDS MUL_1176 NC_008611.1 1262365 1262682 D cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 1262365..1262682 Mycobacterium ulcerans Agy99 4554049 YP_905226.1 CDS MUL_1177 NC_008611.1 1263037 1263726 D cytoplasmic protein; PEP phosphonomutase 1263037..1263726 Mycobacterium ulcerans Agy99 4554050 YP_905227.1 CDS hsp NC_008611.1 1263801 1264259 R cytoplasmic protein; thought to be involved in the initiation step of translation at high temperature. bound to 30S ribosomal subunit. possibly a molecular chaperone. in M. tuberculosis H37Rv seems to be regulated positively by SigE and negatively by HspR.; heat shock protein Hsp complement(1263801..1264259) Mycobacterium ulcerans Agy99 4554051 YP_905228.1 CDS nirD NC_008611.1 1267066 1267422 D cytoplasmic protein; involved in nitrate assimilation (denitrification); required for activity of the reductase [catalytic activity: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O]; nitrite reductase [NAD(P)H] small subunit NirD 1267066..1267422 Mycobacterium ulcerans Agy99 4554053 YP_905229.1 CDS MUL_1181 NC_008611.1 1267559 1268014 R hypothetical protein complement(1267559..1268014) Mycobacterium ulcerans Agy99 4554054 YP_905230.1 CDS MUL_1182 NC_008611.1 1268071 1268832 R cytoplasmic protein; hypothetical protein complement(1268071..1268832) Mycobacterium ulcerans Agy99 4554055 YP_905231.1 CDS MUL_1183 NC_008611.1 1268823 1269974 R catalyzes the formation of uroporphyrinogen-III from hydroxymethylbilane; bifunctional uroporphyrinogen-III synthetase/response regulator domain-containing protein complement(1268823..1269974) Mycobacterium ulcerans Agy99 4554056 YP_905232.1 CDS aac NC_008611.1 1271684 1272229 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; confers resistance to aminoglycosides (gentamicin, tobramycin, dibekacin, netilmicin, and 6'-N- ethylnetilmicin); aminoglycoside 2'-N-acetyltransferase Aac complement(1271684..1272229) Mycobacterium ulcerans Agy99 4554058 YP_905233.1 CDS MUL_1186 NC_008611.1 1272256 1273125 R cytoplasmic protein; urea amidolyase-related protein complement(1272256..1273125) Mycobacterium ulcerans Agy99 4554059 YP_905234.1 CDS MUL_1187 NC_008611.1 1273141 1273812 R cytoplasmic protein; hypothetical protein complement(1273141..1273812) Mycobacterium ulcerans Agy99 4554060 YP_905235.1 CDS MUL_1188 NC_008611.1 1273925 1274917 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; thought to be involved in iron transport across the membrane (import); periplasmic iron-transport lipoprotein complement(1273925..1274917) Mycobacterium ulcerans Agy99 4554061 YP_905236.1 CDS narU NC_008611.1 1275171 1276568 D membrane protein; involved in excretion of nitrite produced by the dissimilatory reduction of nitrate. responsible for the translocation of the substrate across the membrane.; integral membrane nitrite extrusion protein NarU 1275171..1276568 Mycobacterium ulcerans Agy99 4554062 YP_905237.1 CDS MUL_1190 NC_008611.1 1276569 1277840 R cytoplasmic protein; hypothetical protein complement(1276569..1277840) Mycobacterium ulcerans Agy99 4554063 YP_905238.1 CDS fadD2 NC_008611.1 1277899 1279572 D activates fatty acids by binding to coenzyme A; acyl-CoA synthetase 1277899..1279572 Mycobacterium ulcerans Agy99 4554064 YP_905239.1 CDS MUL_1193 NC_008611.1 1280018 1281370 R membrane protein; hypothetical protein complement(1280018..1281370) Mycobacterium ulcerans Agy99 4554065 YP_905240.1 CDS fadE6 NC_008611.1 1281606 1283792 R cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE6 complement(1281606..1283792) Mycobacterium ulcerans Agy99 4554066 YP_905241.1 CDS serA3 NC_008611.1 1283849 1284811 R cytoplasmic protein; involved at the first committed step in the 'phosphorylated' pathway of L-serine biosynthesis [catalytic activity: 3-phosphoglycerate + NAD(+) = 3- phosphohydroxypyruvate + NADH]; D-3-phosphoglycerate dehydrogenase complement(1283849..1284811) Mycobacterium ulcerans Agy99 4554067 YP_905242.1 CDS MUL_1196 NC_008611.1 1284808 1285941 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(1284808..1285941) Mycobacterium ulcerans Agy99 4554068 YP_905243.1 CDS MUL_1197 NC_008611.1 1285943 1286551 R cytoplasmic protein; could be involved in transcriptional mechanism.; transcriptional regulatory protein complement(1285943..1286551) Mycobacterium ulcerans Agy99 4554069 YP_905244.1 CDS MUL_1198 NC_008611.1 1286642 1287223 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS); cytoplasmic protein; hypothetical protein 1286642..1287223 Mycobacterium ulcerans Agy99 4554070 YP_905245.1 CDS MUL_1199 NC_008611.1 1287230 1287778 D membrane protein; hypothetical protein 1287230..1287778 Mycobacterium ulcerans Agy99 4554071 YP_905246.1 CDS MUL_1200 NC_008611.1 1287732 1288388 R cytoplasmic protein; could be involved in transcriptional mechanism.; TetR family transcriptional regulator complement(1287732..1288388) Mycobacterium ulcerans Agy99 4554407 YP_905247.1 CDS MUL_1201 NC_008611.1 1288513 1289415 D hypothetical protein 1288513..1289415 Mycobacterium ulcerans Agy99 4554408 YP_905248.1 CDS MUL_1202 NC_008611.1 1289688 1291244 D PPE3; secreted protein; PPE family protein 1289688..1291244 Mycobacterium ulcerans Agy99 4554409 YP_905249.1 CDS MUL_5109 NC_008611.1 1292296 1292520 D cytoplasmic protein; hypothetical protein 1292296..1292520 Mycobacterium ulcerans Agy99 4554411 YP_905250.1 CDS MUL_1204 NC_008611.1 1292627 1294489 D Detected in the cytoplasmic and the membrane fraction by 2D-LC-MS/MS.; cytoplasmic protein; hypothetical protein 1292627..1294489 Mycobacterium ulcerans Agy99 4554412 YP_905251.1 CDS MUL_1205 NC_008611.1 1294486 1296087 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 1294486..1296087 Mycobacterium ulcerans Agy99 4554413 YP_905252.1 CDS MUL_1206 NC_008611.1 1296084 1300085 D membrane protein; hypothetical protein 1296084..1300085 Mycobacterium ulcerans Agy99 4554414 YP_905253.1 CDS MUL_1207 NC_008611.1 1300110 1300418 D PE5; Detected in the secreted fraction by proteomics.; secreted protein; PE family protein 1300110..1300418 Mycobacterium ulcerans Agy99 4554415 YP_905254.1 CDS MUL_1208 NC_008611.1 1300421 1301974 D PPE4; membrane protein; PPE family protein 1300421..1301974 Mycobacterium ulcerans Agy99 4554416 YP_905255.1 CDS esxG NC_008611.1 1302011 1302304 D secreted protein; EsaT-6 like protein EsxG 1302011..1302304 Mycobacterium ulcerans Agy99 4554417 YP_905256.1 CDS esxH NC_008611.1 1302333 1302623 D membrane protein; EsaT-6 like protein EsxH 1302333..1302623 Mycobacterium ulcerans Agy99 4554418 YP_905257.1 CDS MUL_1211 NC_008611.1 1302632 1303528 D Detected in the membrane fraction by proteomics.; cytoplasmic protein; hypothetical protein 1302632..1303528 Mycobacterium ulcerans Agy99 4554419 YP_905258.1 CDS MUL_1212 NC_008611.1 1303600 1305018 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; transmembrane protein 1303600..1305018 Mycobacterium ulcerans Agy99 4554420 YP_905259.1 CDS mycP3 NC_008611.1 1305015 1306439 D Detected in the membrane fraction by proteomics (LC- MS/MS) Also detected in the cytoplasm by proteomics.; membrane protein; thought to have proteolytic activity.; membrane-anchored mycosin MycP3 1305015..1306439 Mycobacterium ulcerans Agy99 4554421 YP_905260.1 CDS MUL_1214 NC_008611.1 1306492 1307442 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; transmembrane protein 1306492..1307442 Mycobacterium ulcerans Agy99 4554422 YP_905261.1 CDS MUL_1215 NC_008611.1 1307429 1308649 R membrane protein; hypothetical protein complement(1307429..1308649) Mycobacterium ulcerans Agy99 4554423 YP_905262.1 CDS tam NC_008611.1 1308806 1309597 D catalyzes the formation of (E)-3-(methoxycarbonyl)pent-2-enedioate and S-adenosyl-L-homocysteine from S-adenosyl-L-methionine and trans-aconitate; trans-aconitate 2-methyltransferase 1308806..1309597 Mycobacterium ulcerans Agy99 4554424 YP_905263.1 CDS MUL_1217 NC_008611.1 1309615 1310970 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; sulfatase complement(1309615..1310970) Mycobacterium ulcerans Agy99 4554425 YP_905264.1 CDS MUL_1218 NC_008611.1 1311079 1311729 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase complement(1311079..1311729) Mycobacterium ulcerans Agy99 4554426 YP_905265.1 CDS MUL_1219 NC_008611.1 1311722 1311922 R Detected in the membrane fraction by proteomics; membrane protein; membrane protein complement(1311722..1311922) Mycobacterium ulcerans Agy99 4554427 YP_905266.1 CDS MUL_1220 NC_008611.1 1311915 1312172 D membrane protein; hypothetical protein 1311915..1312172 Mycobacterium ulcerans Agy99 4554428 YP_905267.1 CDS MUL_1221 NC_008611.1 1312156 1312638 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(1312156..1312638) Mycobacterium ulcerans Agy99 4554429 YP_905268.1 CDS MUL_1222 NC_008611.1 1312695 1313363 D membrane protein; integral membrane protein 1312695..1313363 Mycobacterium ulcerans Agy99 4554430 YP_905269.1 CDS MUL_1223 NC_008611.1 1313469 1314125 D secreted protein; hypothetical protein 1313469..1314125 Mycobacterium ulcerans Agy99 4554431 YP_905270.1 CDS MUL_1224 NC_008611.1 1314134 1314640 R cytoplasmic protein; hypothetical protein complement(1314134..1314640) Mycobacterium ulcerans Agy99 4554432 YP_905271.1 CDS MUL_1225 NC_008611.1 1314700 1315929 D cytoplasmic protein; hypothetical protein 1314700..1315929 Mycobacterium ulcerans Agy99 4554433 YP_905272.1 CDS MUL_1226 NC_008611.1 1316082 1317962 D membrane protein; hypothetical protein 1316082..1317962 Mycobacterium ulcerans Agy99 4554434 YP_905273.1 CDS MUL_1227 NC_008611.1 1318184 1319542 D membrane protein; hypothetical protein 1318184..1319542 Mycobacterium ulcerans Agy99 4554435 YP_905274.1 CDS MUL_1231 NC_008611.1 1329776 1330147 D cytoplasmic protein; hypothetical protein 1329776..1330147 Mycobacterium ulcerans Agy99 4554437 YP_905275.1 CDS MUL_1233 NC_008611.1 1331902 1333845 R cytoplasmic protein; function unknown, but seems involved in metabolism of small branched-chain fatty acids and macrolide antibiotic production. catalyses the alpha, beta- dehydrogenetion of acyl-CoA esters and transfer electrons to ETF, the electron transfer protein.; acyl-CoA dehydrogenase complement(1331902..1333845) Mycobacterium ulcerans Agy99 4554439 YP_905276.1 CDS MUL_1234 NC_008611.1 1334046 1334705 D membrane protein 1334046..1334705 Mycobacterium ulcerans Agy99 4554440 YP_905277.1 CDS MUL_1236 NC_008611.1 1335782 1336828 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 1335782..1336828 Mycobacterium ulcerans Agy99 4554442 YP_905278.1 CDS MUL_1237 NC_008611.1 1337211 1339733 R membrane protein; thought to be involved in active transport of undeterminated substrate (possibly lipooligosaccharide) across the membrane. responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane.; transmembrane ABC transporter ATP-binding protein complement(1337211..1339733) Mycobacterium ulcerans Agy99 4554443 YP_905279.1 CDS MUL_1238 NC_008611.1 1339885 1340361 R cytoplasmic protein; hypothetical protein complement(1339885..1340361) Mycobacterium ulcerans Agy99 4554444 YP_905280.1 CDS MUL_1239 NC_008611.1 1340480 1342552 D membrane protein; probably involved in active transport accross the membrane. thought to be responsible for energy coupling to the transport system and the translocation of the substrate across the membrane.; transmembrane ATP-binding protein ABC transorter 1340480..1342552 Mycobacterium ulcerans Agy99 4554509 YP_905281.1 CDS MUL_1240 NC_008611.1 1342549 1343409 D membrane protein; hypothetical protein 1342549..1343409 Mycobacterium ulcerans Agy99 4554510 YP_905282.1 CDS MUL_1241 NC_008611.1 1343406 1343858 R cytoplasmic protein; involved in transcriptional mechanism.; AsnC family transcriptional regulator complement(1343406..1343858) Mycobacterium ulcerans Agy99 4554511 YP_905283.1 CDS MUL_1242 NC_008611.1 1343982 1344857 D cytoplasmic protein; hypothetical protein 1343982..1344857 Mycobacterium ulcerans Agy99 4554512 YP_905284.1 CDS rocD NC_008611.1 1344854 1346098 D cytoplasmic protein; involved in arginine metabolism [catalytic activity: L-ornithine + a 2-OXO acid = L-glutamate 5- semialdehyde + an L-amino acid]; ornithine aminotransferase RocD1 and RocD2 1344854..1346098 Mycobacterium ulcerans Agy99 4554513 YP_905285.1 CDS rocE NC_008611.1 1346128 1347597 D membrane protein; thought to be involved in transport of cationic amino acid (especially arginine and ornithine) across the membrane. responsible for the translocation of the substrate across the membrane.; cationic amino acid transport integral membrane protein RocE 1346128..1347597 Mycobacterium ulcerans Agy99 4554514 YP_905286.1 CDS MUL_1245 NC_008611.1 1347594 1348466 D Detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; hypothetical protein 1347594..1348466 Mycobacterium ulcerans Agy99 4554515 YP_905287.1 CDS uspC NC_008611.1 1348497 1349825 R membrane protein; thought to be involved in active transport of sugar across the membrane (import); periplasmic sugar-binding lipoprotein UspC complement(1348497..1349825) Mycobacterium ulcerans Agy99 4554516 YP_905288.1 CDS uspB NC_008611.1 1349822 1350628 R secreted protein; thought to be involved in active transport of sugar across the membrane (import) responsible for the translocation of the substrate across the membrane.; sugar-transport integral membrane protein ABC transporter UspB complement(1349822..1350628) Mycobacterium ulcerans Agy99 4554517 YP_905289.1 CDS uspA NC_008611.1 1350648 1351520 R Also detected in the cytoplasm by proteomics.; membrane protein; thought to be involved in active transport of sugar across the membrane (import) responsible for the translocation of the substrate across the membrane.; sugar-transport integral membrane protein ABC transporter UspA complement(1350648..1351520) Mycobacterium ulcerans Agy99 4554518 YP_905290.1 CDS MUL_1249 NC_008611.1 1351555 1353072 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS) Two isoforms were also detected in the cytoplasm.; membrane protein; hypothetical protein 1351555..1353072 Mycobacterium ulcerans Agy99 4554519 YP_905291.1 CDS MUL_1250 NC_008611.1 1353069 1354442 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; hypothetical protein 1353069..1354442 Mycobacterium ulcerans Agy99 4554520 YP_905292.1 CDS MUL_1252 NC_008611.1 1355342 1356115 D secreted protein; hypothetical protein 1355342..1356115 Mycobacterium ulcerans Agy99 4554522 YP_905293.1 CDS MUL_1253 NC_008611.1 1356283 1356591 D cytoplasmic protein; hypothetical protein 1356283..1356591 Mycobacterium ulcerans Agy99 4554523 YP_905294.1 CDS MUL_1254 NC_008611.1 1356588 1359200 R membrane protein; metal cation-transporting ATPase; possibly catalyzes the transport of a undeterminated metal cation with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + undeterminated metal cation(in) = ADP + phosphate + undeterminated metal cation(out)]; metal cation transporter p-type ATPase a complement(1356588..1359200) Mycobacterium ulcerans Agy99 4554524 YP_905295.1 CDS MUL_1255 NC_008611.1 1359441 1359968 D cytoplasmic protein; hypothetical protein 1359441..1359968 Mycobacterium ulcerans Agy99 4554525 YP_905296.1 CDS MUL_1256 NC_008611.1 1360070 1360504 R cytoplasmic protein; hypothetical protein complement(1360070..1360504) Mycobacterium ulcerans Agy99 4554526 YP_905297.1 CDS MUL_1259 NC_008611.1 1361169 1361369 D cytoplasmic protein; function unknown.; hypothetical protein 1361169..1361369 Mycobacterium ulcerans Agy99 4552000 YP_905298.1 CDS MUL_1260 NC_008611.1 1361754 1361861 D cytoplasmic protein; hypothetical protein 1361754..1361861 Mycobacterium ulcerans Agy99 4552001 YP_905299.1 CDS MUL_1261 NC_008611.1 1362331 1363035 D cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 1362331..1363035 Mycobacterium ulcerans Agy99 4552002 YP_905300.1 CDS MUL_1262 NC_008611.1 1363060 1363377 D cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 1363060..1363377 Mycobacterium ulcerans Agy99 4552003 YP_905301.1 CDS MUL_1263 NC_008611.1 1363529 1364863 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 1363529..1364863 Mycobacterium ulcerans Agy99 4552004 YP_905302.1 CDS MUL_1264 NC_008611.1 1365078 1365560 D cytoplasmic protein; contains PTPS, 6-pyruvoyl tetrahydropterin synthase domain. 6-pyruvoyl tetrahydrobiopterin synthase catalyses the conversion of dihydroneopterin triphosphate to 6- pyruvoyl tetrahydropterin, the second of three enzymatic steps in the synthesis of tetrahydrobiopterin from GTP. the functional enzyme is a hexamer of identical subunits.; 6-pyruvoyl tetrahydrobiopterin synthase 1365078..1365560 Mycobacterium ulcerans Agy99 4552005 YP_905303.1 CDS MUL_1265 NC_008611.1 1366310 1367266 D cytoplasmic protein; hypothetical protein 1366310..1367266 Mycobacterium ulcerans Agy99 4552006 YP_905304.1 CDS MUL_1266 NC_008611.1 1367290 1367745 D cytoplasmic protein; hypothetical protein 1367290..1367745 Mycobacterium ulcerans Agy99 4552007 YP_905305.1 CDS MUL_1267 NC_008611.1 1367771 1369105 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(1367771..1369105) Mycobacterium ulcerans Agy99 4552008 YP_905306.1 CDS MUL_1268 NC_008611.1 1369347 1370081 D membrane protein; function unknown, predicted hydrolase (had superfamily) domain.; hypothetical protein 1369347..1370081 Mycobacterium ulcerans Agy99 4552009 YP_905307.1 CDS MUL_1269 NC_008611.1 1370187 1370921 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown, in general, cation channels are transport proteins responsible for the movement of cations through the membrane.; ion transport protein 1370187..1370921 Mycobacterium ulcerans Agy99 4552010 YP_905308.1 CDS MUL_1270 NC_008611.1 1370681 1371280 R Potential pseudogene due to a point mutation, removing a stop codon and now the CDS overlaps the C-term of a CDS on the opposite strand.; membrane protein; transmembrane protein complement(1370681..1371280) Mycobacterium ulcerans Agy99 4552011 YP_905309.1 CDS MUL_1271 NC_008611.1 1371277 1371654 R membrane protein; transmembrane protein complement(1371277..1371654) Mycobacterium ulcerans Agy99 4552012 YP_905310.1 CDS MUL_1273 NC_008611.1 1371853 1372251 R cytoplasmic protein; hypothetical protein complement(1371853..1372251) Mycobacterium ulcerans Agy99 4552013 YP_905311.1 CDS lppN NC_008611.1 1372248 1372781 R secreted protein; lipoprotein LppN complement(1372248..1372781) Mycobacterium ulcerans Agy99 4552014 YP_905312.1 CDS MUL_1275 NC_008611.1 1372818 1373414 R cytoplasmic protein; hypothetical protein complement(1372818..1373414) Mycobacterium ulcerans Agy99 4552015 YP_905313.1 CDS MUL_1277 NC_008611.1 1374417 1374734 R cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 complement(1374417..1374734) Mycobacterium ulcerans Agy99 4550118 YP_905314.1 CDS MUL_1278 NC_008611.1 1374759 1375463 R cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 complement(1374759..1375463) Mycobacterium ulcerans Agy99 4550119 YP_905315.1 CDS MUL_1280 NC_008611.1 1376289 1376915 D cytoplasmic protein; hypothetical protein 1376289..1376915 Mycobacterium ulcerans Agy99 4550120 YP_905316.1 CDS aofH_1 NC_008611.1 1376924 1378297 R Also detected in the membrane fraction by proteomics (LC-MS/MS); cytoplasmic protein; possibly catalyzes the oxidative deamination: oxidize on primary amines, and perhaps on secondary and tertiary amines [catalytic activity: RCH(2)NH(2) + H(2)O + O(2) = RchO + NH(3) + H(2)O(2)] must have important function in metabolism. supposed involved in stationary- phase survival.; flavin-containing monoamine oxidase AofH complement(1376924..1378297) Mycobacterium ulcerans Agy99 4550121 YP_905317.1 CDS cyp124A1 NC_008611.1 1378331 1379632 R cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.; cytochrome P450 124A1, Cyp124A1 complement(1378331..1379632) Mycobacterium ulcerans Agy99 4550122 YP_905318.1 CDS MUL_1286 NC_008611.1 1383567 1383854 R cytoplasmic protein; function unknown.; hypothetical protein complement(1383567..1383854) Mycobacterium ulcerans Agy99 4550126 YP_905319.1 CDS MUL_1287 NC_008611.1 1383949 1385019 R cytoplasmic protein; hypothetical protein complement(1383949..1385019) Mycobacterium ulcerans Agy99 4550127 YP_905320.1 CDS MUL_1288 NC_008611.1 1385057 1386010 R Detected in the membrane and cytoplamic fractions by proteomics.; cytoplasmic protein; oxidoreduction; short chain dehydrogenase complement(1385057..1386010) Mycobacterium ulcerans Agy99 4550128 YP_905321.1 CDS MUL_1289 NC_008611.1 1386093 1387595 D secreted protein; function unknown; thought to be involved in lipid metabolism.; hypothetical protein 1386093..1387595 Mycobacterium ulcerans Agy99 4550129 YP_905322.1 CDS MUL_1290 NC_008611.1 1387592 1388341 D membrane protein; function unknown.; hypothetical protein 1387592..1388341 Mycobacterium ulcerans Agy99 4550130 YP_905323.1 CDS MUL_1291 NC_008611.1 1388404 1389009 R cytoplasmic protein; hypothetical protein complement(1388404..1389009) Mycobacterium ulcerans Agy99 4550131 YP_905324.1 CDS adhE2 NC_008611.1 1389027 1390112 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); secreted protein; oxido-reduction; zinc-dependent alcohol dehydrogenase AdhE2 complement(1389027..1390112) Mycobacterium ulcerans Agy99 4550132 YP_905325.1 CDS MUL_1293 NC_008611.1 1390243 1391313 D cytoplasmic protein; possibly involved in transcriptional regulation; transcriptional regulatory protein 1390243..1391313 Mycobacterium ulcerans Agy99 4550133 YP_905326.1 CDS MUL_1294 NC_008611.1 1391326 1392144 D cytoplasmic protein; hypothetical protein 1391326..1392144 Mycobacterium ulcerans Agy99 4550134 YP_905327.1 CDS MUL_1295 NC_008611.1 1392298 1392810 D cytoplasmic protein; hypothetical protein 1392298..1392810 Mycobacterium ulcerans Agy99 4550135 YP_905328.1 CDS MUL_1297 NC_008611.1 1393458 1394393 R involved in the biosynthesis of phosphatidylinositol mannosides (PIMs); the enzyme from Mycobacterium tuberculosis can phosphorylate a variety of amphipathic lipids; diacylglycerol kinase complement(1393458..1394393) Mycobacterium ulcerans Agy99 4550710 YP_905329.1 CDS MUL_1298 NC_008611.1 1394417 1395997 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; electron acceptor; flavoprotein complement(1394417..1395997) Mycobacterium ulcerans Agy99 4550711 YP_905330.1 CDS MUL_1299 NC_008611.1 1396045 1396614 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; possibly involved in transcriptional regulation; transcriptional regulatory protein 1396045..1396614 Mycobacterium ulcerans Agy99 4550712 YP_905331.1 CDS glpD1 NC_008611.1 1396614 1398149 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in aerobic respiration and oxydation of glycerol. reduces an acceptor and generates glycerone phosphate from SN-glycerol 3-phosphate. possibly play a role in metabolism of riboflavin, fad,FMN [catalytic activity: SN-glycerol 3-phosphate + acceptor = glycerone phosphate + reduced acceptor]; glycerol-3-phosphate dehydrogenase 1396614..1398149 Mycobacterium ulcerans Agy99 4550713 YP_905332.1 CDS MUL_1301 NC_008611.1 1398230 1399045 R cytoplasmic protein; hypothetical protein complement(1398230..1399045) Mycobacterium ulcerans Agy99 4550714 YP_905333.1 CDS accD6 NC_008611.1 1399243 1400664 R Detected in the membrane fraction by proteomics; membrane protein; involved in fatty acid biosynthesis (mycolic acids synthesis) [catalytic activity: ATP + propionyl-CoA + CO(2) + H(2)O = ADP + orthophosphate + methylmalonyl- CoA]; acetyl/propionyl CoA carboxylase subunit beta complement(1399243..1400664) Mycobacterium ulcerans Agy99 4550715 YP_905334.1 CDS kasB NC_008611.1 1400702 1401955 R FabF; beta-ketoacyl-ACP synthase II, KASII; catalyzes a condensation reaction in fatty acid biosynthesis: addition of an acyl acceptor of two carbons from malonyl-ACP; required for the elongation of short-chain unsaturated acyl-ACP; 3-oxoacyl-(acyl carrier protein) synthase II complement(1400702..1401955) Mycobacterium ulcerans Agy99 4550716 YP_905335.1 CDS kasA NC_008611.1 1402037 1403287 R FabF; beta-ketoacyl-ACP synthase II, KASII; catalyzes a condensation reaction in fatty acid biosynthesis: addition of an acyl acceptor of two carbons from malonyl-ACP; required for the elongation of short-chain unsaturated acyl-ACP; 3-oxoacyl-(acyl carrier protein) synthase II complement(1402037..1403287) Mycobacterium ulcerans Agy99 4550717 YP_905336.1 CDS acpP NC_008611.1 1403284 1403631 R carries the fatty acid chain in fatty acid biosynthesis; acyl carrier protein complement(1403284..1403631) Mycobacterium ulcerans Agy99 4550718 YP_905337.1 CDS fabD NC_008611.1 1403704 1404612 R membrane protein; catalyzes malonyl-CoA-acp transacylase (McaT) activity using holo-AcpM as substrate for transacylation [catalytic activity: malonyl-CoA + [acyl-carrier protein] = CoA + malonyl-[acyl-carrier protein]; malonyl CoA-acyl carrier protein transacylase FabD complement(1403704..1404612) Mycobacterium ulcerans Agy99 4550719 YP_905338.1 CDS MUL_1307 NC_008611.1 1404774 1406084 R cytoplasmic protein; hypothetical protein complement(1404774..1406084) Mycobacterium ulcerans Agy99 4550720 YP_905339.1 CDS aceE NC_008611.1 1406218 1409007 R E1 component; part of pyruvate dehydrogenase; forms a complex with DlaT and LpdC; pyruvate dehydrogenase subunit E1 complement(1406218..1409007) Mycobacterium ulcerans Agy99 4550721 YP_905340.1 CDS MUL_1309 NC_008611.1 1409192 1409794 D N-term truncated (missing first 69aa) wrt to M. tuberculosis; membrane protein; hypothetical protein 1409192..1409794 Mycobacterium ulcerans Agy99 4550722 YP_905341.1 CDS MUL_1310 NC_008611.1 1409851 1410297 D cytoplasmic protein; hypothetical protein 1409851..1410297 Mycobacterium ulcerans Agy99 4550723 YP_905342.1 CDS ahpE NC_008611.1 1410297 1410758 D cytoplasmic protein; detoxification of organic peroxides.; peroxiredoxin AhpE 1410297..1410758 Mycobacterium ulcerans Agy99 4550724 YP_905343.1 CDS MUL_1313 NC_008611.1 1410897 1412447 R cytoplasmic protein; hypothetical protein complement(1410897..1412447) Mycobacterium ulcerans Agy99 4550726 YP_905344.1 CDS MUL_1315 NC_008611.1 1413009 1413815 D membrane protein; function unknown, possibly involved in transport of metal ions across the membrane.; hypothetical protein 1413009..1413815 Mycobacterium ulcerans Agy99 4551377 YP_905345.1 CDS MUL_1316 NC_008611.1 1413836 1414651 R cytoplasmic protein; hypothetical protein complement(1413836..1414651) Mycobacterium ulcerans Agy99 4551378 YP_905346.1 CDS cobD NC_008611.1 1414765 1415706 D CobD; CbiD in Salmonella; converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group; cobalamin biosynthesis protein 1414765..1415706 Mycobacterium ulcerans Agy99 4551379 YP_905347.1 CDS MUL_1318 NC_008611.1 1415688 1416512 R membrane protein; function unknown: may be involved in the ability to survive in macrophages.; transmembrane protein complement(1415688..1416512) Mycobacterium ulcerans Agy99 4551380 YP_905348.1 CDS ptpA NC_008611.1 1416512 1417006 R cytoplasmic protein; involved in signal transduction (via dephosphorylation) can dephosphorylated in vitro the phosphotyrosine residue of myelin basic protein (MBP) at ph 7.0 [catalytic activity: protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.; phosphotyrosine protein phosphatase PtpA complement(1416512..1417006) Mycobacterium ulcerans Agy99 4551381 YP_905349.1 CDS MUL_1320 NC_008611.1 1416999 1417406 R cytoplasmic protein; hypothetical protein complement(1416999..1417406) Mycobacterium ulcerans Agy99 4551382 YP_905350.1 CDS cobC NC_008611.1 1417906 1418961 D cytoplasmic protein; involved in cobalamin biosynthesis; hypothetical protein 1417906..1418961 Mycobacterium ulcerans Agy99 4551383 YP_905351.1 CDS MUL_1322 NC_008611.1 1418958 1420094 D cytoplasmic protein; hypothetical protein 1418958..1420094 Mycobacterium ulcerans Agy99 4551384 YP_905352.1 CDS MUL_1323 NC_008611.1 1420117 1420854 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein 1420117..1420854 Mycobacterium ulcerans Agy99 4551385 YP_905353.1 CDS MUL_1324 NC_008611.1 1420851 1421975 D cytoplasmic protein; bifunctional RNase H/acid phosphatase 1420851..1421975 Mycobacterium ulcerans Agy99 4551386 YP_905354.1 CDS MUL_1328 NC_008611.1 1426446 1428038 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS; cytoplasmic protein; hypothetical protein complement(1426446..1428038) Mycobacterium ulcerans Agy99 4551390 YP_905355.1 CDS MUL_1329 NC_008611.1 1428303 1428623 D membrane protein; hypothetical protein 1428303..1428623 Mycobacterium ulcerans Agy99 4551391 YP_905356.1 CDS panB NC_008611.1 1428631 1429524 R catalyzes the formation of tetrahydrofolate and 2-dehydropantoate from 5,10-methylenetetrahydrofolate and 3-methyl-2-oxobutanoate; 3-methyl-2-oxobutanoate hydroxymethyltransferase complement(1428631..1429524) Mycobacterium ulcerans Agy99 4551392 YP_905357.1 CDS MUL_1332 NC_008611.1 1431122 1432678 D secreted protein; function unknown; thought to hydrolyze peptides and/or proteins.; exported protease 1431122..1432678 Mycobacterium ulcerans Agy99 4554072 YP_905358.1 CDS ctaD_1 NC_008611.1 1432822 1434558 D membrane protein; cytochrome C oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. electrons originating in cytochrome C are transferred via the copper a center of subunit 2 and HemE a of subunit 1 to the bimetallic center formed by HemE A3 and copper B [catalytic activity: 4 ferrocytochrome C + O(2) = 2 H(2)O + 4 ferricytochrome C]; cytochrome C oxidase polypeptide I CtaD 1432822..1434558 Mycobacterium ulcerans Agy99 4554073 YP_905359.1 CDS MUL_1334 NC_008611.1 1434619 1436187 D secreted protein; function unknown; thought to hydrolyze peptides and/or proteins.; exported protease 1434619..1436187 Mycobacterium ulcerans Agy99 4554074 YP_905360.1 CDS glnA2 NC_008611.1 1436277 1437617 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; involved in glutamine biosynthesis [catalytic activity: ATP + L-glutamate + NH(3) = ADP + glutamine + orthophosphate]; glutamine synthetase 1436277..1437617 Mycobacterium ulcerans Agy99 4554075 YP_905361.1 CDS glnE NC_008611.1 1437688 1440675 D catalyzes the ATP-dependent addition of AMP to a subunit of glutamine synthetase; also catalyzes the reverse reaction - deadenylation; adenylation/deadenylation of glutamine synthetase subunits is important for the regulation of this enzyme; bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase 1437688..1440675 Mycobacterium ulcerans Agy99 4554076 YP_905362.1 CDS MUL_1338 NC_008611.1 1441534 1442181 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; hypothetical protein 1441534..1442181 Mycobacterium ulcerans Agy99 4554078 YP_905363.1 CDS MUL_1339 NC_008611.1 1442226 1444406 R membrane protein; function unknown. has fibronectin-binding activity (could thus mediate bacterial attachment to host cells) thought to be expressed during infection.; PE-PGRS family protein complement(1442226..1444406) Mycobacterium ulcerans Agy99 4554079 YP_905364.1 CDS glnA1 NC_008611.1 1444670 1446106 R Detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; involved in glutamine biosynthesis [catalytic activity: ATP + L-glutamate + NH(3) = ADP + glutamine + orthophosphate]; glutamine synthetase GlnA1 complement(1444670..1446106) Mycobacterium ulcerans Agy99 4554080 YP_905365.1 CDS MUL_1341 NC_008611.1 1446349 1446771 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 1446349..1446771 Mycobacterium ulcerans Agy99 4554081 YP_905366.1 CDS MUL_1342 NC_008611.1 1446822 1447574 R membrane protein; transmembrane protein complement(1446822..1447574) Mycobacterium ulcerans Agy99 4554082 YP_905367.1 CDS lipA NC_008611.1 1447602 1448576 R catalyzes the radical-mediated insertion of two sulfur atoms into an acyl carrier protein (ACP) bound to an octanoyl group to produce a lipoyl group; lipoyl synthase complement(1447602..1448576) Mycobacterium ulcerans Agy99 4554083 YP_905368.1 CDS lipB NC_008611.1 1448573 1449298 R lipoyl/octanoyltransferase; catalyzes the transfer of the lipoyl/octanoyl moiety of lipoyl/octanoyl-ACP onto lipoate-dependent enzymes like pyruvate dehydrogenase and the glycine cleavage system H protein; lipoate-protein ligase B complement(1448573..1449298) Mycobacterium ulcerans Agy99 4554084 YP_905369.1 CDS MUL_1345 NC_008611.1 1449570 1451300 D membrane protein; thought to be involved in active transport of drugs across the membrane (export): multidrugs resistance by an export mechanism. responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane.; drugs-transport transmembrane ATP-binding protein ABC transporter 1449570..1451300 Mycobacterium ulcerans Agy99 4554085 YP_905370.1 CDS MUL_1347 NC_008611.1 1453681 1454073 D membrane protein; hypothetical protein 1453681..1454073 Mycobacterium ulcerans Agy99 4554087 YP_905371.1 CDS ilvB1_2 NC_008611.1 1454098 1455774 R membrane protein; involved in valine and isoleucine biosynthesis (at the first step) [catalytic activity: 2-acetolactate + CO(2) = 2 pyruvate]; acetolactate synthase large subunit IlvB complement(1454098..1455774) Mycobacterium ulcerans Agy99 4554088 YP_905372.1 CDS MUL_1349 NC_008611.1 1455774 1456433 R cytoplasmic protein; function unknown, may have methyltransferase activity; hypothetical protein complement(1455774..1456433) Mycobacterium ulcerans Agy99 4554089 YP_905373.1 CDS wrbA NC_008611.1 1457336 1458064 R cytoplasmic protein; multimeric flavodoxin WrbA complement(1457336..1458064) Mycobacterium ulcerans Agy99 4551949 YP_905374.1 CDS MUL_1352 NC_008611.1 1458615 1458842 R cytoplasmic protein; hypothetical protein complement(1458615..1458842) Mycobacterium ulcerans Agy99 4551950 YP_905375.1 CDS MUL_1353 NC_008611.1 1458889 1459476 R cytoplasmic protein; the sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released.; alternative RNA polymerase sigma factor complement(1458889..1459476) Mycobacterium ulcerans Agy99 4551951 YP_905376.1 CDS MUL_1354 NC_008611.1 1459677 1460213 D cytoplasmic protein; hypothetical protein 1459677..1460213 Mycobacterium ulcerans Agy99 4551952 YP_905377.1 CDS MUL_1355 NC_008611.1 1460336 1460548 R secreted protein; hypothetical protein complement(1460336..1460548) Mycobacterium ulcerans Agy99 4551953 YP_905378.1 CDS MUL_1357 NC_008611.1 1462775 1464109 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 1462775..1464109 Mycobacterium ulcerans Agy99 4551955 YP_905379.1 CDS MUL_1359 NC_008611.1 1466235 1467281 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 1466235..1467281 Mycobacterium ulcerans Agy99 4551957 YP_905380.1 CDS MUL_1363 NC_008611.1 1469784 1471004 D membrane protein; PE-PGRS family protein 1469784..1471004 Mycobacterium ulcerans Agy99 4551960 YP_905381.1 CDS add NC_008611.1 1471013 1472101 R catalyzes the formation of inosine from adenosine; adenosine deaminase complement(1471013..1472101) Mycobacterium ulcerans Agy99 4551961 YP_905382.1 CDS deoA NC_008611.1 1472098 1473399 R Catalyzes the reversible phosphorolysis of thymidine, deoxyuridine and their analogues to their respective bases and 2-deoxyribose 1-phosphate; thymidine phosphorylase complement(1472098..1473399) Mycobacterium ulcerans Agy99 4552552 YP_905383.1 CDS cdd NC_008611.1 1473396 1473794 R Reclaims exogenous and endogenous cytidine and 2'-deoxycytidine molecules for UMP synthesis; cytidine deaminase complement(1473396..1473794) Mycobacterium ulcerans Agy99 4552553 YP_905384.1 CDS sdhC NC_008611.1 1474082 1474420 D membrane protein; involved in tricarboxylic acid cycle. mono-heme cytochrome of the succinate dehydrogenase complex.; succinate dehydrogenase (cytochrome B-556 subunit) SdhC 1474082..1474420 Mycobacterium ulcerans Agy99 4552554 YP_905385.1 CDS sdhD NC_008611.1 1474462 1474884 D membrane protein; involved in tricarboxylic acid cycle. putative hydrophobic component of the succinate dehydrogenase complex. could be required to anchor the catalytic components to the cytoplasmic membrane.; succinate dehydrogenase (hydrophobic membrane anchor subunit) SdhD 1474462..1474884 Mycobacterium ulcerans Agy99 4552555 YP_905386.1 CDS sdhA NC_008611.1 1474913 1476667 D part of four member succinate dehydrogenase enzyme complex that forms a trimeric complex (trimer of tetramers); SdhA/B are the catalytic subcomplex and can exhibit succinate dehydrogenase activity in the absence of SdhC/D which are the membrane components and form cytochrome b556; SdhC binds ubiquinone; oxidizes succinate to fumarate while reducing ubiquinone to ubiquinol; succinate dehydrogenase flavoprotein subunit 1474913..1476667 Mycobacterium ulcerans Agy99 4552556 YP_905387.1 CDS sdhB NC_008611.1 1476673 1477479 D part of four member succinate dehydrogenase enzyme complex that forms a trimeric complex (trimer of tetramers); SdhA/B are the catalytic subcomplex and can exhibit succinate dehydrogenase activity in the absence of SdhC/D which are the membrane components and form cytochrome b556; SdhC binds ubiquinone; oxidizes succinate to fumarate while reducing ubiquinone to ubiquinol; the catalytic subunits are similar to fumarate reductase; succinate dehydrogenase iron-sulfur subunit 1476673..1477479 Mycobacterium ulcerans Agy99 4552557 YP_905388.1 CDS MUL_1371 NC_008611.1 1477801 1478118 R cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 complement(1477801..1478118) Mycobacterium ulcerans Agy99 4552558 YP_905389.1 CDS MUL_1372 NC_008611.1 1478143 1478847 R cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 complement(1478143..1478847) Mycobacterium ulcerans Agy99 4552559 YP_905390.1 CDS MUL_1373 NC_008611.1 1479341 1479787 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; transmembrane protein complement(1479341..1479787) Mycobacterium ulcerans Agy99 4552560 YP_905391.1 CDS xthA NC_008611.1 1479931 1480725 R Detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; involved in base excision repair. apurinic- apyrimidinic endonuclease. supposed to remove the damaged DNA at cytosines and guanines by cleaving at the 3' side of the ap site by a beta-elimination reaction. possibly exhibites 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'- repair diesterase and ribonuclease H activities [catalytic activity: degradation of double-stranded DNA. it acts progressively in a 3'- to 5'-direction, releasing 5'- phosphomononucleotides]; exodeoxyribonuclease III protein XthA complement(1479931..1480725) Mycobacterium ulcerans Agy99 4552561 YP_905392.1 CDS def NC_008611.1 1481661 1482254 R cleaves off formyl group from N-terminal methionine residues of newly synthesized proteins; binds iron(2+); peptide deformylase complement(1481661..1482254) Mycobacterium ulcerans Agy99 4552563 YP_905393.1 CDS MUL_1377 NC_008611.1 1482288 1482812 D cytoplasmic protein; hypothetical protein 1482288..1482812 Mycobacterium ulcerans Agy99 4552564 YP_905394.1 CDS MUL_1378 NC_008611.1 1482868 1483359 D Detected in the membrane fraction and secreted fractions by proteomics. (2D-LC-MS/MS); membrane protein; tuberculin-like protein 1482868..1483359 Mycobacterium ulcerans Agy99 4552565 YP_905395.1 CDS sodC NC_008611.1 1483432 1484148 D Detected in the membrane fraction by proteomics; secreted protein; destroys radicals which are normally produced within the cells and are toxic to biological systems [catalytic activity: 2 superoxide + 2 H+ = O2 + H2O2]; periplasmic superoxide dismutase [Cu-Zn] SodC 1483432..1484148 Mycobacterium ulcerans Agy99 4552566 YP_905396.1 CDS MUL_1380 NC_008611.1 1484102 1485280 D ATP-dependent carboxylate-amine ligase; carboxylate-amine ligase 1484102..1485280 Mycobacterium ulcerans Agy99 4552567 YP_905397.1 CDS MUL_1381 NC_008611.1 1485385 1486047 R cytoplasmic protein; involved in transcriptional mechanism.; GntR family transcriptional regulator complement(1485385..1486047) Mycobacterium ulcerans Agy99 4552568 YP_905398.1 CDS MUL_1382 NC_008611.1 1486184 1487470 D membrane protein; function unknown, possibly involved in inorganic ion transport.; MFS transporter 1486184..1487470 Mycobacterium ulcerans Agy99 4552569 YP_905399.1 CDS MUL_1383 NC_008611.1 1487608 1488264 D cytoplasmic protein; hypothetical protein 1487608..1488264 Mycobacterium ulcerans Agy99 4552570 YP_905400.1 CDS MUL_1384 NC_008611.1 1488271 1490448 R membrane protein; ATPase; possibly catalyzes the transport of undeterminated substrate with hydrolyse of ATP [catalytic activity: ATP + H(2)O + undeterminated substrate(in) = ADP + phosphate + undeterminated substrate(out)]; ATPase complement(1488271..1490448) Mycobacterium ulcerans Agy99 4552534 YP_905401.1 CDS pssA NC_008611.1 1490445 1491320 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in phospholipid biosynthesis. generates phosphatidylserine [catalytic activity: CDP-diacylglycerol + L-serine = CMP + O-SN-phosphatidyl-L-serine]; CDP-diacylglycerol--serine O-phosphatidyltransferase complement(1490445..1491320) Mycobacterium ulcerans Agy99 4552535 YP_905402.1 CDS psd NC_008611.1 1491317 1492039 R catalyzes the decarboxylaton of phospatidyl-L-sering to phosphatidylethanolamine; phosphatidylserine decarboxylase complement(1491317..1492039) Mycobacterium ulcerans Agy99 4552536 YP_905403.1 CDS MUL_1388 NC_008611.1 1494204 1495250 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(1494204..1495250) Mycobacterium ulcerans Agy99 4552538 YP_905404.1 CDS moeA2 NC_008611.1 1495965 1497182 R cytoplasmic protein; involved in molybdenum cofactor biosynthesis: involved in the biosynthesis of a demolybdo-cofactor (molybdopterin), necessary for molybdo-enzymes (by similarity); molybdopterin biosynthesis protein MoeA2 complement(1495965..1497182) Mycobacterium ulcerans Agy99 4552539 YP_905405.1 CDS MUL_1391 NC_008611.1 1497201 1498139 R cytoplasmic protein; function unknown, possibly involved in cellular metabolism.; short chain dehydrogenase complement(1497201..1498139) Mycobacterium ulcerans Agy99 4552540 YP_905406.1 CDS MUL_1392 NC_008611.1 1498186 1498707 R cytoplasmic protein; hypothetical protein complement(1498186..1498707) Mycobacterium ulcerans Agy99 4552541 YP_905407.1 CDS groEL NC_008611.1 1498999 1500624 D 60 kDa chaperone family; promotes refolding of misfolded polypeptides especially under stressful conditions; forms two stacked rings of heptamers to form a barrel-shaped 14mer; ends can be capped by GroES; misfolded proteins enter the barrel where they are refolded when GroES binds; many bacteria have multiple copies of the groEL gene which are active under different environmental conditions; the B.japonicum protein in this cluster is expressed constitutively; in Rhodobacter, Corynebacterium and Rhizobium this protein is essential for growth; chaperonin GroEL 1498999..1500624 Mycobacterium ulcerans Agy99 4552542 YP_905408.1 CDS MUL_1394 NC_008611.1 1500708 1501127 R cytoplasmic protein; hypothetical protein complement(1500708..1501127) Mycobacterium ulcerans Agy99 4552543 YP_905409.1 CDS MUL_1395 NC_008611.1 1501164 1502636 R PPE10; membrane protein; PPE family protein complement(1501164..1502636) Mycobacterium ulcerans Agy99 4552544 YP_905410.1 CDS MUL_1396 NC_008611.1 1502732 1504444 R cytoplasmic protein; hydrolizes a specific D-amino acid.; D-amino acid aminohydrolase complement(1502732..1504444) Mycobacterium ulcerans Agy99 4552545 YP_905411.1 CDS MUL_1397 NC_008611.1 1504547 1505062 D cytoplasmic protein; hypothetical protein 1504547..1505062 Mycobacterium ulcerans Agy99 4552546 YP_905412.1 CDS MUL_1398 NC_008611.1 1505126 1505920 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 1505126..1505920 Mycobacterium ulcerans Agy99 4552547 YP_905413.1 CDS MUL_1399 NC_008611.1 1505932 1506630 R membrane protein; hypothetical protein complement(1505932..1506630) Mycobacterium ulcerans Agy99 4552548 YP_905414.1 CDS sigK NC_008611.1 1506627 1507190 R Member of the extracytoplasmic function sigma factors which are active under specific conditions; binds with the catalytic core of RNA polymerase to produce the holoenzyme and directs bacterial core RNA polymerase to specific promoter elements to initiate transcription; in M. bovis this protein has been shown to be involved in expression of antigenic proteins; RNA polymerase sigma factor SigK complement(1506627..1507190) Mycobacterium ulcerans Agy99 4552549 YP_905415.1 CDS MUL_1401 NC_008611.1 1507236 1508009 R membrane protein; transmembrane protein complement(1507236..1508009) Mycobacterium ulcerans Agy99 4552550 YP_905416.1 CDS ufaA1 NC_008611.1 1508027 1509313 R cytoplasmic protein; transfers a methylene group from S-adenosyl-L- methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge [catalytic activity: S-adenosyl-L- methionine + phospholipid olefinic fatty acid = S-adenosyl- L-homocysteine + phospholipid cyclopropane fatty acid]; cyclopropane-fatty-acyl-phospholipid synthase complement(1508027..1509313) Mycobacterium ulcerans Agy99 4552551 YP_905417.1 CDS MUL_1403 NC_008611.1 1509310 1509954 R cytoplasmic protein; hypothetical protein complement(1509310..1509954) Mycobacterium ulcerans Agy99 4552518 YP_905418.1 CDS MUL_1404 NC_008611.1 1510020 1511336 R membrane protein; oxidoreductase complement(1510020..1511336) Mycobacterium ulcerans Agy99 4552519 YP_905419.1 CDS MUL_1406 NC_008611.1 1512229 1513563 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 1512229..1513563 Mycobacterium ulcerans Agy99 4552521 YP_905420.1 CDS MUL_1407 NC_008611.1 1513923 1514627 D cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 1513923..1514627 Mycobacterium ulcerans Agy99 4552522 YP_905421.1 CDS MUL_1408 NC_008611.1 1514652 1514969 D cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 1514652..1514969 Mycobacterium ulcerans Agy99 4552523 YP_905422.1 CDS MUL_1410 NC_008611.1 1515962 1517296 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(1515962..1517296) Mycobacterium ulcerans Agy99 4552525 YP_905423.1 CDS MUL_1411 NC_008611.1 1518095 1518442 R cytoplasmic protein; hypothetical protein complement(1518095..1518442) Mycobacterium ulcerans Agy99 4552526 YP_905424.1 CDS MUL_1413 NC_008611.1 1518718 1519167 R Detected in the secreted protein fraction by 2D-LC- MS/MS.; secreted protein; hypothetical protein complement(1518718..1519167) Mycobacterium ulcerans Agy99 4552527 YP_905425.1 CDS echA2 NC_008611.1 1519234 1520148 R catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase complement(1519234..1520148) Mycobacterium ulcerans Agy99 4552528 YP_905426.1 CDS MUL_1416 NC_008611.1 1522423 1523946 D cytoplasmic protein; interconversion aldehyde and acid [catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH]; aldehyde dehydrogenase 1522423..1523946 Mycobacterium ulcerans Agy99 4552530 YP_905427.1 CDS MUL_1417 NC_008611.1 1523946 1524446 D cytoplasmic protein; hypothetical protein 1523946..1524446 Mycobacterium ulcerans Agy99 4552531 YP_905428.1 CDS MUL_1418 NC_008611.1 1524506 1524745 D membrane protein; hypothetical protein 1524506..1524745 Mycobacterium ulcerans Agy99 4552532 YP_905429.1 CDS MUL_1419 NC_008611.1 1524738 1525307 D membrane protein; transmembrane protein 1524738..1525307 Mycobacterium ulcerans Agy99 4552533 YP_905430.1 CDS MUL_1421 NC_008611.1 1525894 1526940 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 1525894..1526940 Mycobacterium ulcerans Agy99 4552486 YP_905431.1 CDS MUL_1423 NC_008611.1 1527708 1528973 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; dehydrogenase 1527708..1528973 Mycobacterium ulcerans Agy99 4552488 YP_905432.1 CDS MUL_1424 NC_008611.1 1529017 1531545 R membrane protein; thought to be involved in active transport of undeterminated substrate (possibly lipooligosaccharide) across the membrane. responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane.; transmembrane ABC transporter ATP-binding protein complement(1529017..1531545) Mycobacterium ulcerans Agy99 4552489 YP_905433.1 CDS folD NC_008611.1 1535863 1536708 D catalyzes the formation of 5,10-methenyltetrahydrofolate from 5,10-methylenetetrahydrofolate and subsequent formation of 10-formyltetrahydrofolate from 5,10-methenyltetrahydrofolate; bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase 1535863..1536708 Mycobacterium ulcerans Agy99 4552492 YP_905434.1 CDS MUL_1429 NC_008611.1 1536705 1536998 D membrane protein; hypothetical protein 1536705..1536998 Mycobacterium ulcerans Agy99 4552493 YP_905435.1 CDS MUL_1430 NC_008611.1 1537002 1537418 R Detected in the secreted fraction by proteomics.; secreted protein; hypothetical protein complement(1537002..1537418) Mycobacterium ulcerans Agy99 4552494 YP_905436.1 CDS MUL_1431 NC_008611.1 1537672 1539114 D secreted protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 1537672..1539114 Mycobacterium ulcerans Agy99 4552495 YP_905437.1 CDS fadD28_1 NC_008611.1 1539362 1541206 D activates fatty acids by binding to coenzyme A; acyl-CoA synthetase 1539362..1541206 Mycobacterium ulcerans Agy99 4552496 YP_905438.1 CDS MUL_1434 NC_008611.1 1549194 1549490 R secreted protein; hypothetical protein complement(1549194..1549490) Mycobacterium ulcerans Agy99 4552498 YP_905439.1 CDS MUL_1435 NC_008611.1 1549552 1549899 R secreted protein; exported protein complement(1549552..1549899) Mycobacterium ulcerans Agy99 4552499 YP_905440.1 CDS MUL_1436 NC_008611.1 1550039 1550776 R cytoplasmic protein; causes methylation; methyltransferase complement(1550039..1550776) Mycobacterium ulcerans Agy99 4552500 YP_905441.1 CDS metX NC_008611.1 1550770 1551909 R Catalyzes the conversion of acetyl-CoA and L-homoserine to CoA and O-acetyl-L-homoserine; homoserine O-acetyltransferase complement(1550770..1551909) Mycobacterium ulcerans Agy99 4552501 YP_905442.1 CDS metC NC_008611.1 1551921 1553270 R catalyzes the formation of L-methionine and acetate from O-acetyl-L-homoserine and methanethiol; O-acetylhomoserine aminocarboxypropyltransferase complement(1551921..1553270) Mycobacterium ulcerans Agy99 4552502 YP_905443.1 CDS MUL_1440 NC_008611.1 1554837 1555538 R membrane protein; hypothetical protein complement(1554837..1555538) Mycobacterium ulcerans Agy99 4552470 YP_905444.1 CDS trpS NC_008611.1 1555795 1556814 D catalyzes a two-step reaction, first charging a tryptophan molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; tryptophanyl-tRNA synthetase 1555795..1556814 Mycobacterium ulcerans Agy99 4552471 YP_905445.1 CDS MUL_1442 NC_008611.1 1556819 1557847 D membrane protein; integral membrane protein 1556819..1557847 Mycobacterium ulcerans Agy99 4552472 YP_905446.1 CDS nagA NC_008611.1 1557844 1559010 R membrane protein; involved in N-acetyl glucosamine utilization pathway [catalytic activity: N-acetyl-D-glucosamine 6- phosphate + H(2)O = D-glucosamine 6-phosphate + acetate]; n-acetylglucosamine-6-phosphate deacetylase NagA complement(1557844..1559010) Mycobacterium ulcerans Agy99 4552473 YP_905447.1 CDS sugI NC_008611.1 1559007 1560356 R membrane protein; thought to be involved in transport of sugar across the membrane. responsible for the translocation of the substrate across the membrane.; sugar-transport integral membrane protein SugI complement(1559007..1560356) Mycobacterium ulcerans Agy99 4552474 YP_905448.1 CDS dacC NC_008611.1 1560558 1561805 R secreted protein; involved in peptidoglycan synthesis (at final stages) hydrolyzes the bound D-alanyl-D-alanine [catalytic activity: D-alanyl-D-alanine + H(2)O = 2 D- alanine]; penicillin-binding protein DacC complement(1560558..1561805) Mycobacterium ulcerans Agy99 4552475 YP_905449.1 CDS MUL_1446 NC_008611.1 1561927 1562565 D secreted protein; hypothetical protein 1561927..1562565 Mycobacterium ulcerans Agy99 4552476 YP_905450.1 CDS MUL_1447 NC_008611.1 1562672 1564873 D cytoplasmic protein; possible inter-alpha-trypsin inhibitor family heavy chain-related protein-hypothetical secreted or membrane- associated protein containing vWFA domain.; hypothetical protein 1562672..1564873 Mycobacterium ulcerans Agy99 4552477 YP_905451.1 CDS MUL_1450 NC_008611.1 1567392 1568603 D membrane protein; PE-PGRS family protein 1567392..1568603 Mycobacterium ulcerans Agy99 4552480 YP_905452.1 CDS MUL_1454 NC_008611.1 1571380 1572426 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(1571380..1572426) Mycobacterium ulcerans Agy99 4552484 YP_905453.1 CDS esxP_1 NC_008611.1 1572858 1573154 D Detected in the cytoplasmic and membrane fractions by 2D-LC-MS/MS.; secreted protein; EsaT-6 like protein EsxP 1572858..1573154 Mycobacterium ulcerans Agy99 4552453 YP_905454.1 CDS esxN_1 NC_008611.1 1573217 1573501 D Also detected in the cytoplasmic fraction by 2D-LC- MS/MS.; secreted protein; EsaT-6 like protein EsxN 1573217..1573501 Mycobacterium ulcerans Agy99 4552454 YP_905455.1 CDS dgt NC_008611.1 1575842 1577119 D dGTPase family type 2 subfamily; presumably hydrolyzes dGTP to deoxyguanosine and triphosphate; deoxyguanosinetriphosphate triphosphohydrolase-like protein 1575842..1577119 Mycobacterium ulcerans Agy99 4552456 YP_905456.1 CDS dnaG NC_008611.1 1577161 1579131 D synthesizes RNA primers at the replication forks; DNA primase 1577161..1579131 Mycobacterium ulcerans Agy99 4552457 YP_905457.1 CDS MUL_1460 NC_008611.1 1579146 1579391 R hypothetical protein complement(1579146..1579391) Mycobacterium ulcerans Agy99 4552458 YP_905458.1 CDS MUL_1462 NC_008611.1 1579758 1580210 R secreted protein; function unknown, contains N-term signal peptide indicative of secreted protein.; hypothetical protein complement(1579758..1580210) Mycobacterium ulcerans Agy99 4552460 YP_905459.1 CDS thiX NC_008611.1 1580238 1580666 R secreted protein; thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions.; thioredoxin ThiX complement(1580238..1580666) Mycobacterium ulcerans Agy99 4552461 YP_905460.1 CDS cysE NC_008611.1 1580677 1581363 R cytoplasmic protein; involved in cysteine biosynthesis [catalytic activity: acetyl-CoA + L-serine = CoA + O-acetyl-L- serine]; serine acetyltransferase CysE complement(1580677..1581363) Mycobacterium ulcerans Agy99 4552462 YP_905461.1 CDS cysK1 NC_008611.1 1581366 1582298 R Also detected in the membrane fraction by proteomics.; cytoplasmic protein; involved in cysteine biosynthesis [catalytic activity: O3-acetyl-L-serine + H(2)S = L-cysteine + acetate]; cysteine synthase a CysK1 complement(1581366..1582298) Mycobacterium ulcerans Agy99 4552463 YP_905462.1 CDS MUL_1466 NC_008611.1 1582295 1582711 R hypothetical protein complement(1582295..1582711) Mycobacterium ulcerans Agy99 4552464 YP_905463.1 CDS mez NC_008611.1 1583019 1584665 R malic enzyme; oxaloacetate-decarboxylating; NAD-dependent; catalyzes the formation of pyruvate form malate; malate dehydrogenase complement(1583019..1584665) Mycobacterium ulcerans Agy99 4552465 YP_905464.1 CDS MUL_1468 NC_008611.1 1585082 1585987 D membrane protein; thought to be involved in a transport system across the membrane (perhaps drug transport): responsible for the translocation of the substrate across the membrane.; integral membrane transport protein 1585082..1585987 Mycobacterium ulcerans Agy99 4552466 YP_905465.1 CDS MUL_1469 NC_008611.1 1586263 1587309 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 1586263..1587309 Mycobacterium ulcerans Agy99 4552467 YP_905466.1 CDS MUL_1471 NC_008611.1 1587623 1588957 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 1587623..1588957 Mycobacterium ulcerans Agy99 4552433 YP_905467.1 CDS MUL_1472 NC_008611.1 1590742 1592343 R membrane protein; thought to play an essential roles in regulation of cellular metabolism by catalysing the synthesis of a second messenger, camp [catalytic activity: ATP = 3',5'- cyclic AMP + pyrophosphate]; adenylate cyclase complement(1590742..1592343) Mycobacterium ulcerans Agy99 4552434 YP_905468.1 CDS fdhF NC_008611.1 1592995 1597203 R cytoplasmic protein; decomposes formic acid to hydrogen and carbon dioxide under anaerobic conditions in the absence of exogenous electron acceptors [catalytic activity: formate + NAD(+) = CO(2) + NADH]; formate dehydrogenase H FdhF complement(1592995..1597203) Mycobacterium ulcerans Agy99 4552435 YP_905469.1 CDS lppP NC_008611.1 1597621 1598124 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; lipoprotein LppP 1597621..1598124 Mycobacterium ulcerans Agy99 4552436 YP_905470.1 CDS ctpD NC_008611.1 1598916 1600895 D membrane protein; cation-transporting ATPase; possibly catalyzes the transport of a cation (possibly cadmium) with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + cation(in) = ADP + phosphate + cation(out)]; cation transporter p-type ATPase D CtpD 1598916..1600895 Mycobacterium ulcerans Agy99 4552438 YP_905471.1 CDS trxA NC_008611.1 1600990 1601346 D cytoplasmic protein; thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions.; thioredoxin TrxA 1600990..1601346 Mycobacterium ulcerans Agy99 4552439 YP_905472.1 CDS trxB1 NC_008611.1 1601432 1601761 D cytoplasmic protein; thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions.; thioredoxin TrxB1 1601432..1601761 Mycobacterium ulcerans Agy99 4552440 YP_905473.1 CDS echA12 NC_008611.1 1601845 1602654 D Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase 1601845..1602654 Mycobacterium ulcerans Agy99 4552441 YP_905474.1 CDS MUL_1481 NC_008611.1 1602679 1604307 D membrane protein; probable macrolide-transport ATP-binding protein ABC transporter. thought to be involved in active transport of macrolide across the membrane.; ABC transporter ATP-binding protein 1602679..1604307 Mycobacterium ulcerans Agy99 4552442 YP_905475.1 CDS MUL_1482 NC_008611.1 1604392 1604583 D cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein 1604392..1604583 Mycobacterium ulcerans Agy99 4552443 YP_905476.1 CDS MUL_1483 NC_008611.1 1604585 1605148 R cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein complement(1604585..1605148) Mycobacterium ulcerans Agy99 4552444 YP_905477.2 CDS acn NC_008611.1 1605158 1607977 R Catalyzes the conversion of citrate to isocitrate; aconitate hydratase complement(1605158..1607977) Mycobacterium ulcerans Agy99 4552445 YP_905478.1 CDS MUL_1485 NC_008611.1 1608253 1608831 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 1608253..1608831 Mycobacterium ulcerans Agy99 4552446 YP_905479.1 CDS MUL_1486 NC_008611.1 1609063 1610472 D secreted protein; function unknown, similar to a hypothetical invasion protein. possibly an exported protein with unusually long signal sequence. NLP/P60 family protein; cell wall-associated hydrolase 1609063..1610472 Mycobacterium ulcerans Agy99 4552447 YP_905480.1 CDS inv2 NC_008611.1 1610498 1611217 D secreted protein; function unknown. homology to cell wall-associated hydrolases. possibly an exported protein of the NLP/P60 family.; invasion protein Inv2 1610498..1611217 Mycobacterium ulcerans Agy99 4552448 YP_905481.1 CDS moxR1 NC_008611.1 1611416 1612531 D Detected in the cytoplamic and membrane fractions by LC-MS/MS. Also detected in the extracellular matrix by proteomics.; membrane protein; involved in transcriptional mechanism.; transcriptional regulatory protein, MoxR1 1611416..1612531 Mycobacterium ulcerans Agy99 4552449 YP_905482.1 CDS MUL_1489 NC_008611.1 1612546 1613502 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 1612546..1613502 Mycobacterium ulcerans Agy99 4552450 YP_905483.1 CDS MUL_1490 NC_008611.1 1613537 1614544 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 1613537..1614544 Mycobacterium ulcerans Agy99 4552451 YP_905484.1 CDS fabG1 NC_008611.1 1614659 1615426 D Detected in the membrane fraction by proteomics (LC- MS/MS) Also detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; involved in the fatty acid biosynthesis pathway (first reduction step) (mycolic acid biosynthesis); reduces KasA/KasB products [catalytic activity: (3R)-3- hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl- [acyl-carrier protein] + NADPH]; 3-oxoacyl-ACP reductase 1614659..1615426 Mycobacterium ulcerans Agy99 4552452 YP_905485.1 CDS inhA NC_008611.1 1615505 1616314 D Catalyzes a key regulatory step in fatty acid biosynthesis; enoyl-(acyl carrier protein) reductase 1615505..1616314 Mycobacterium ulcerans Agy99 4552376 YP_905486.1 CDS hemH NC_008611.1 1616321 1617343 D protoheme ferro-lyase; catalyzes the insertion of a ferrous ion into protoporphyrin IX to form protoheme; involved in protoheme biosynthesis; in some organisms this protein is membrane-associated while in others it is cytosolic; ferrochelatase 1616321..1617343 Mycobacterium ulcerans Agy99 4552377 YP_905487.1 CDS MUL_1494 NC_008611.1 1617350 1618345 R cytoplasmic protein; hypothetical protein complement(1617350..1618345) Mycobacterium ulcerans Agy99 4552378 YP_905488.1 CDS MUL_1495 NC_008611.1 1618312 1619646 D cytoplasmic protein; required for the transposition of insertion element IS2606; transposase for IS2606 1618312..1619646 Mycobacterium ulcerans Agy99 4552379 YP_905489.1 CDS MUL_1496 NC_008611.1 1619804 1620238 D membrane protein; function unknown. contains Cdd match to COG1585, COG1585, membrane protein implicated in regulation of membrane protease activity [posttranslational modification, protein turnover, chaperones / intracellular trafficking and secretion]; hypothetical protein 1619804..1620238 Mycobacterium ulcerans Agy99 4552380 YP_905490.1 CDS MUL_1498 NC_008611.1 1621425 1621769 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 1621425..1621769 Mycobacterium ulcerans Agy99 4552382 YP_905491.1 CDS MUL_1499 NC_008611.1 1621766 1622200 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(1621766..1622200) Mycobacterium ulcerans Agy99 4552383 YP_905492.1 CDS MUL_1500 NC_008611.1 1622266 1622880 R cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulator complement(1622266..1622880) Mycobacterium ulcerans Agy99 4552384 YP_905493.1 CDS MUL_1501 NC_008611.1 1623755 1624498 D cytoplasmic protein; contains N-terminal Cdd pfam00072, response_reg, response regulator receiver domain. this domain receives the signal from the sensor partner in bacterial two- component systems. it is usually found N-terminal to a DNA binding effector domain. C-terminal conatins Cdd pfam00486, trans_reg_C, transcriptional regulatory protein, C terminal.; two component regulator receiver domain-containing protein 1623755..1624498 Mycobacterium ulcerans Agy99 4552385 YP_905494.1 CDS MUL_1502 NC_008611.1 1624498 1626018 D membrane protein; two component system response sensor kinase contains Cdd pfam02518, hATPase_C, histidine kinase-, DNA gyrase B-, and Hsp90-like ATPase; Cdd pfam00512, HisKA, His kinase a (phosphoacceptor) domain. dimerisation and phosphoacceptor domain of histidine kinases.; two component regulator - sensor kinase 1624498..1626018 Mycobacterium ulcerans Agy99 4552386 YP_905495.1 CDS MUL_1503 NC_008611.1 1626023 1626838 R membrane protein; hypothetical protein complement(1626023..1626838) Mycobacterium ulcerans Agy99 4552387 YP_905496.1 CDS MUL_1504 NC_008611.1 1627062 1627841 R membrane protein; conserved membrane protein of unknown function. similar to proteins from many organisms containing Cdd COG0398; uncharacterized conserved protein [function unknown]; hypothetical protein complement(1627062..1627841) Mycobacterium ulcerans Agy99 4552388 YP_905497.1 CDS mutA NC_008611.1 1628029 1629906 D membrane protein; involved in propionic acid fermentation. catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid- cycle intermediates [catalytic activity: (R)-2-methyl-3- oxopropanoyl-CoA = succinyl- CoA]; methylmalonyl-CoA mutase small subunit, MutA 1628029..1629906 Mycobacterium ulcerans Agy99 4552389 YP_905498.1 CDS mutB NC_008611.1 1629912 1632173 D MDM; functions in conversion of succinate to propionate; methylmalonyl-CoA mutase 1629912..1632173 Mycobacterium ulcerans Agy99 4552390 YP_905499.1 CDS MUL_1507 NC_008611.1 1632262 1633251 D functions in transport of arginine/ornithine; inner membrane ATPase that cleaves ATP and phosphorylates two periplasmic proteins that function as two distinct transport systems, the AO (arginine and ornithine) and LAO (lysine, arginine, and ornithine) periplasmic binding proteins; arginine/ornithine transport system ATPase 1632262..1633251 Mycobacterium ulcerans Agy99 4552391 YP_905500.1 CDS MUL_1509 NC_008611.1 1634621 1634833 R cytoplasmic protein; function unknown. contains Cdd pfam07311, DUF1458, protein of unknown function (DUF1458) this family consists of several hypothetical bacterial proteins as well as one archaeal sequence. members of this family are typically of around 70 residues in length. the function of this family is unknown.; hypothetical protein complement(1634621..1634833) Mycobacterium ulcerans Agy99 4552393 YP_905501.1 CDS MUL_1510 NC_008611.1 1634961 1635740 R cytoplasmic protein; hypothetical protein complement(1634961..1635740) Mycobacterium ulcerans Agy99 4552360 YP_905502.1 CDS MUL_1511 NC_008611.1 1635989 1637245 D cytoplasmic protein; possibly involved in polysaccharide biosynthesis [catalytic activity: UDP-glucose + 2 NAD+ + H2O = UDP- glucuronate + 2 NADH] belongs to UDP-glucose/GDP-mannose dehydrogenase family; UDP-glucose 6-dehydrogenase 1635989..1637245 Mycobacterium ulcerans Agy99 4552361 YP_905503.1 CDS MUL_1512 NC_008611.1 1637242 1638261 D cytoplasmic protein; involved in galactofuranosyl biosynthesis: converts UDO-GlcP to UDP-GalP [catalytic activity: UDP- glucopyranose = UDP-galactopyranose]; UDP-glucose 4-epimerase 1637242..1638261 Mycobacterium ulcerans Agy99 4552362 YP_905504.1 CDS MUL_1518 NC_008611.1 1642065 1642769 D cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 1642065..1642769 Mycobacterium ulcerans Agy99 4552366 YP_905505.1 CDS MUL_1519 NC_008611.1 1642800 1643111 D cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 1642800..1643111 Mycobacterium ulcerans Agy99 4552367 YP_905506.1 CDS MUL_1520 NC_008611.1 1643319 1644650 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 1643319..1644650 Mycobacterium ulcerans Agy99 4552368 YP_905507.1 CDS MUL_5113 NC_008611.1 1645717 1645884 D cytoplasmic protein; hypothetical protein 1645717..1645884 Mycobacterium ulcerans Agy99 4552370 YP_905508.1 CDS MUL_1522 NC_008611.1 1646073 1646306 D cytoplasmic protein; hypothetical protein 1646073..1646306 Mycobacterium ulcerans Agy99 4552371 YP_905509.1 CDS wbbL2 NC_008611.1 1646564 1647331 D membrane protein; possibly involved in cell wall arabinogalactan linker formation: uses dTDP-L-rhamnose as substrate to insert the rhamnosyl residue into the cell wall; rhamnosyl transferase WbbL2 1646564..1647331 Mycobacterium ulcerans Agy99 4552372 YP_905510.1 CDS MUL_1526 NC_008611.1 1648150 1648899 D cytoplasmic protein; function unknown.contains Cdd pfam00535, glycos_transf_2, glycosyl transferase. diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.; glycosyl transferase family protein 1648150..1648899 Mycobacterium ulcerans Agy99 4552374 YP_905511.1 CDS MUL_1527 NC_008611.1 1649017 1649751 R membrane protein; hypothetical protein complement(1649017..1649751) Mycobacterium ulcerans Agy99 4552375 YP_905512.1 CDS MUL_1528 NC_008611.1 1650047 1651093 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 1650047..1651093 Mycobacterium ulcerans Agy99 4552340 YP_905513.1 CDS MUL_1529 NC_008611.1 1651094 1652236 R membrane protein; function unknown. contains Cdd COG1819, glycosyl transferases, related to UDP-glucuronosyltransferase [carbohydrate transport and metabolism / signal transduction mechanisms]; UDP-glycosyltransferase complement(1651094..1652236) Mycobacterium ulcerans Agy99 4552341 YP_905514.1 CDS MUL_1530 NC_008611.1 1652337 1653710 R membrane protein; function unknown. similarity to Cdd pfam03007. uncharacterised protein family (UPF0089) this family of uncharacterised proteins is greatly expanded in mycobacterium tuberculosis. the most conserved region of the proteins contains conserved histidine and aspartate residues suggesting a possible metal binding site suggestive of a protease activity.; hypothetical protein complement(1652337..1653710) Mycobacterium ulcerans Agy99 4552342 YP_905515.1 CDS papA3 NC_008611.1 1653725 1655233 R cytoplasmic protein; function unknown. thought to be involved in lipid metabolism.; polyketide synthase associated protein PapA3 complement(1653725..1655233) Mycobacterium ulcerans Agy99 4552343 YP_905516.1 CDS ileS NC_008611.1 1655932 1659132 D IleRS; catalyzes the formation of isoleucyl-tRNA(Ile) from isoleucine and tRNA(Ile); since isoleucine and other amino acids such as valine are similar, there are additional editing function in this enzyme; one is involved in hydrolysis of activated valine-AMP and the other is involved in deacylation of mischarged Val-tRNA(Ile); there are two active sites, one for aminoacylation and one for editing; class-I aminoacyl-tRNA synthetase family type 2 subfamily; some organisms carry two different copies of this enzyme; in some organisms, the type 2 subfamily is associated with resistance to the antibiotic pseudomonic acid (mupirocin); isoleucyl-tRNA synthetase 1655932..1659132 Mycobacterium ulcerans Agy99 4552344 YP_905517.1 CDS MUL_1534 NC_008611.1 1660244 1660816 R cytoplasmic protein; function unknown. partial Cdd COG0030, KsgA, dimethyladenosine transferase (rRNA methylation) [translation, ribosomal structure and biogenesis]; hypothetical protein complement(1660244..1660816) Mycobacterium ulcerans Agy99 4552346 YP_905518.1 CDS dinP_1 NC_008611.1 1660874 1662295 D involved in translesion DNA polymerization with beta clamp of polymerase III; belongs to Y family of polymerases; does not contain proofreading function; DNA polymerase IV 1660874..1662295 Mycobacterium ulcerans Agy99 4552347 YP_905519.1 CDS ansA NC_008611.1 1662260 1663249 R cytoplasmic protein; conversion of asparagine to aspartate; L-aparaginase AnsA complement(1662260..1663249) Mycobacterium ulcerans Agy99 4552348 YP_905520.1 CDS lspA NC_008611.1 1662953 1663882 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; this protein specifically catalyzes the removal of signal peptides from prolipoproteins [catalytic activity : cleavage of N-terminal leader sequences from membrane prolipoproteins; lipoprotein signal peptidase LspA 1662953..1663882 Mycobacterium ulcerans Agy99 4552349 YP_905521.1 CDS MUL_1538 NC_008611.1 1663879 1664916 D membrane protein; pseudouridine synthase (pseudouridylate synthase) (uracil hydrolyase)[catalytic activity] uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H(2)O.; RluA family pseudouridine synthase 1663879..1664916 Mycobacterium ulcerans Agy99 4552350 YP_905522.1 CDS MUL_1539 NC_008611.1 1664817 1665524 R cytoplasmic protein; function unknown. contains partial Cdd COG2226, UbiE, methylase involved in ubiquinone/menaquinone biosynthesis; hypothetical protein complement(1664817..1665524) Mycobacterium ulcerans Agy99 4552351 YP_905523.1 CDS lprL NC_008611.1 1665883 1666518 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown.; lipoprotein, LprL complement(1665883..1666518) Mycobacterium ulcerans Agy99 4552352 YP_905524.1 CDS glbN NC_008611.1 1666602 1667012 R cytoplasmic protein; oxygen transport. this family of HemE binding proteins are found mainly in bacteria.; globin family protein complement(1666602..1667012) Mycobacterium ulcerans Agy99 4552353 YP_905525.1 CDS MUL_1543 NC_008611.1 1668263 1669066 D Detected in the cytoplasmic fraction; membrane protein; possibly involvement in lipid metabolism; ketoacyl reductase 1668263..1669066 Mycobacterium ulcerans Agy99 4552355 YP_905526.1 CDS MUL_1544 NC_008611.1 1669186 1669617 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); cytoplasmic protein; hypothetical protein 1669186..1669617 Mycobacterium ulcerans Agy99 4552356 YP_905527.1 CDS dnaE NC_008611.1 1669684 1673226 D catalyzes DNA-template-directed extension of the 3'- end of a DNA strand by one nucleotide at a time; main replicative polymerase; DNA polymerase III subunit alpha 1669684..1673226 Mycobacterium ulcerans Agy99 4552357 YP_905528.1 CDS fadD11 NC_008611.1 1673353 1675179 D membrane protein; probably involved in lipid degradation; fatty-acid-CoA ligase FadD11 1673353..1675179 Mycobacterium ulcerans Agy99 4552358 YP_905529.1 CDS plsB1 NC_008611.1 1675194 1677068 D PlsB; catalyzes the formation of 1-acyl-sn-glycerol 3-phosphate by transfering the acyl moiety from acyl-CoA; glycerol-3-phosphate acyltransferase 1675194..1677068 Mycobacterium ulcerans Agy99 4552359 YP_905530.1 CDS MUL_1548 NC_008611.1 1677076 1677306 R secreted protein; function unknown.; hypothetical protein complement(1677076..1677306) Mycobacterium ulcerans Agy99 4552321 YP_905531.1 CDS MUL_1549 NC_008611.1 1677348 1677845 D Detected in the cytoplasmic and secreted fractions by 2D-LC-MS/MS.; secreted protein; function unknown. contains Cdd pfam04075, domain of unknown function (DUF385) family of mycobacterium tuberculosis proteins.; hypothetical protein 1677348..1677845 Mycobacterium ulcerans Agy99 4552322 YP_905532.1 CDS ilvA NC_008611.1 1677889 1679169 D catalyzes the formation of 2-oxobutanoate from L-threonine; biosynthetic; threonine dehydratase 1677889..1679169 Mycobacterium ulcerans Agy99 4552323 YP_905533.1 CDS MUL_1551 NC_008611.1 1679197 1679733 R cytoplasmic protein; hypothetical protein complement(1679197..1679733) Mycobacterium ulcerans Agy99 4552324 YP_905534.1 CDS MUL_1552 NC_008611.1 1679775 1681100 D cytoplasmic protein; involved in transcriptional mechanism.; GntR family transcriptional regulator 1679775..1681100 Mycobacterium ulcerans Agy99 4552325 YP_905535.1 CDS treZ NC_008611.1 1681113 1682876 R cytoplasmic protein; involved in trehalose biosynthesis (protective effect) mycobacteria can produce trehalose from glucose 6- phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway); maltooligosyltrehalose trehalohydrolase, TreZ complement(1681113..1682876) Mycobacterium ulcerans Agy99 4552326 YP_905536.1 CDS treY NC_008611.1 1682873 1685170 R membrane protein; involved in trehalose biosynthesis (protective effect) mycobacteria can produce trehalose from glucose 6- phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway); maltooligosyltrehalose synthase TreY complement(1682873..1685170) Mycobacterium ulcerans Agy99 4552327 YP_905537.1 CDS treX NC_008611.1 1685174 1687351 R cytoplasmic protein; possibly involved in trehalose biosynthesis (protective effect) mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway); maltooligosyltrehalose synthase TreX complement(1685174..1687351) Mycobacterium ulcerans Agy99 4552328 YP_905538.1 CDS MUL_1556 NC_008611.1 1687451 1689616 R membrane protein; hypothetical protein complement(1687451..1689616) Mycobacterium ulcerans Agy99 4552329 YP_905539.1 CDS MUL_1557 NC_008611.1 1689724 1690503 R Detected in the secreted protein fraction by 2D-LC- MS/MS.; secreted protein; possible inv protein, probably exported as has QQAPV repeats at c-terminus.; inv protein complement(1689724..1690503) Mycobacterium ulcerans Agy99 4552330 YP_905540.1 CDS MUL_1558 NC_008611.1 1690849 1691301 R membrane protein; hypothetical protein complement(1690849..1691301) Mycobacterium ulcerans Agy99 4552331 YP_905541.1 CDS bioA NC_008611.1 1691386 1692699 D catalyzes the formation of S-adenosyl-4-methylthionine-2-oxobutanoate and 7,8-diaminononanoate from S-adenosyl-L-methionine and 8-amino-7-oxononanoate; adenosylmethionine-8-amino-7-oxononanoate aminotransferase 1691386..1692699 Mycobacterium ulcerans Agy99 4552332 YP_905542.1 CDS bioF1 NC_008611.1 1692706 1693875 D catalyzes the formation of 8-amino-7-oxononanoate from 6-carboxyhexanoyl-CoA and L-alanine; 8-amino-7-oxononanoate synthase 1692706..1693875 Mycobacterium ulcerans Agy99 4552333 YP_905543.1 CDS bioD NC_008611.1 1693872 1694552 D DTB synthetase; dethiobiotin synthase; involved in production of dethiobiotin from ATP and 7,8-diaminononanoate and carbon dioxide; contains magnesium; dithiobiotin synthetase 1693872..1694552 Mycobacterium ulcerans Agy99 4552334 YP_905544.1 CDS MUL_1562 NC_008611.1 1694554 1695063 D membrane protein; hypothetical protein 1694554..1695063 Mycobacterium ulcerans Agy99 4552335 YP_905545.1 CDS bioB NC_008611.1 1695213 1696265 D catalyzes the formation of biotin from dethiobiotin and sulfur 2 S-adenosyl-L-methionine; biotin synthase 1695213..1696265 Mycobacterium ulcerans Agy99 4552336 YP_905546.1 CDS MUL_1564 NC_008611.1 1696281 1696508 D cytoplasmic protein; hypothetical protein 1696281..1696508 Mycobacterium ulcerans Agy99 4552337 YP_905547.1 CDS MUL_1565 NC_008611.1 1696520 1697203 D membrane protein; hypothetical protein 1696520..1697203 Mycobacterium ulcerans Agy99 4552338 YP_905548.1 CDS MUL_1566 NC_008611.1 1697249 1698589 R membrane protein; hypothetical protein complement(1697249..1698589) Mycobacterium ulcerans Agy99 4552339 YP_905549.1 CDS MUL_1567 NC_008611.1 1698946 1699653 R cytoplasmic protein; function unknown, domain identity to ADP-ribose pyrophosphatases, possible role in nucleotide transport and metabolism.; hypothetical protein complement(1698946..1699653) Mycobacterium ulcerans Agy99 4552303 YP_905550.1 CDS nadA NC_008611.1 1699701 1700759 D 3 different subfamilies; catalyzes the formation of quinolinate from iminoaspartate and dihydroxyacetone phosphate; quinolinate synthetase 1699701..1700759 Mycobacterium ulcerans Agy99 4552304 YP_905551.1 CDS nadB NC_008611.1 1700756 1702330 D catalyzes the formation of oxaloacetate from L-aspartate; L-aspartate oxidase 1700756..1702330 Mycobacterium ulcerans Agy99 4552305 YP_905552.1 CDS nadC NC_008611.1 1702330 1703187 D catalyzes the formation of pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose 1-diphosphate from nictinate D-ribonucleotide; nicotinate-nucleotide pyrophosphorylase 1702330..1703187 Mycobacterium ulcerans Agy99 4552306 YP_905553.1 CDS MUL_1571 NC_008611.1 1703249 1703659 R cytoplasmic protein; hypothetical protein complement(1703249..1703659) Mycobacterium ulcerans Agy99 4552307 YP_905554.1 CDS hisD NC_008611.1 1703744 1705087 D catalyzes the oxidation of L-histidinol to L-histidinaldehyde and then to L-histidine in histidine biosynthesis; functions as a dimer; histidinol dehydrogenase 1703744..1705087 Mycobacterium ulcerans Agy99 4552308 YP_905555.1 CDS hisC1 NC_008611.1 1705084 1706232 D catalyzes the formation of L-histidinol phosphate from imidazole-acetol phosphate and glutamate in histidine biosynthesis; histidinol-phosphate aminotransferase 1705084..1706232 Mycobacterium ulcerans Agy99 4552309 YP_905556.1 CDS hisB NC_008611.1 1706229 1706861 D catalyzes the dehydration of D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate to 3-(imidazol-4-yl)-2-oxopropyl phosphate in histidine biosynthesis; imidazoleglycerol-phosphate dehydratase 1706229..1706861 Mycobacterium ulcerans Agy99 4552310 YP_905557.1 CDS hisH NC_008611.1 1706885 1707490 D with HisF IGPS catalyzes the conversion of phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide ribonucleotide phosphate and glutamine to imidazole-glycerol phosphate, 5-aminoimidazol-4-carboxamide ribonucleotide, and glutamate in histidine biosynthesis; the HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of imidazole-glycerol phosphate and 5-aminoimidazol-4-carboxamide ribonucleotide; imidazole glycerol phosphate synthase subunit HisH 1706885..1707490 Mycobacterium ulcerans Agy99 4552311 YP_905558.1 CDS hisA NC_008611.1 1707502 1708236 D catalyzes the formation of 5-(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino-l- (5-phosphoribosyl)imidazole-4-carboxamide from 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide and the formation of 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate from N-(5-phospho-beta-D-ribosyl)anthranilate; involved in histidine and tryptophan biosynthesis; phosphoribosyl isomerase A 1707502..1708236 Mycobacterium ulcerans Agy99 4552312 YP_905559.1 CDS impA NC_008611.1 1708243 1709055 D cytoplasmic protein; involved in inositol phosphate metabolism. it is responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides. key enzyme of the phosphatidyl inositol signaling pathway [catalytic activity: inositol 1(or 4)-monophosphate + H(2)O = inositol + orthophosphate]; inositol-monophosphatase ImpA 1708243..1709055 Mycobacterium ulcerans Agy99 4552313 YP_905560.1 CDS hisF NC_008611.1 1709057 1709842 D catalyzes the conversion of 5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]- 1-(5-phosphoribosyl)imidazole-4-carboxamideand glutamine to imidazole-glycerol phosphate, 5-aminoimidazol-4-carboxamideribonucleotide and glutamate; the HisF subunit acts as a cyclase; imidazole glycerol phosphate synthase subunit HisF 1709057..1709842 Mycobacterium ulcerans Agy99 4552314 YP_905561.1 CDS hisI NC_008611.1 1709839 1710174 D PR-AMP cyclohydrolase; functions in histidine biosynthesis from PRPP; converts 1-(5-phosphoribosyl)-AMP to 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxyamide during the histidine biosynthesis pathway; binds zinc and magnesium; forms homodimers; phosphoribosyl-AMP cyclohydrolase 1709839..1710174 Mycobacterium ulcerans Agy99 4552315 YP_905562.1 CDS MUL_1580 NC_008611.1 1710297 1711388 R cytoplasmic protein; function unknown.; deacylase complement(1710297..1711388) Mycobacterium ulcerans Agy99 4552316 YP_905563.1 CDS MUL_1582 NC_008611.1 1711705 1713039 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 1711705..1713039 Mycobacterium ulcerans Agy99 4552318 YP_905564.1 CDS MUL_1584 NC_008611.1 1713718 1714764 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 1713718..1714764 Mycobacterium ulcerans Agy99 4552320 YP_905565.1 CDS bcpB NC_008611.1 1721808 1722266 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; peroxide detoxification; peroxidoxin BcpB complement(1721808..1722266) Mycobacterium ulcerans Agy99 4552111 YP_905566.1 CDS trpE NC_008611.1 1722317 1723855 D with component II, the glutamine amidotransferase, catalyzes the formation of anthranilate from chorismate and glutamine; anthranilate synthase component I 1722317..1723855 Mycobacterium ulcerans Agy99 4552112 YP_905567.1 CDS MUL_1590 NC_008611.1 1723848 1724543 D membrane protein; hypothetical protein 1723848..1724543 Mycobacterium ulcerans Agy99 4552113 YP_905568.1 CDS trpC NC_008611.1 1724633 1725451 D involved in tryptophan biosynthesis; amino acid biosynthesis; converts 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate to C(1)-(3-indolyl)-glycerol 3-phosphate and carbon dioxide and water; indole-3-glycerol-phosphate synthase 1724633..1725451 Mycobacterium ulcerans Agy99 4552114 YP_905569.1 CDS trpB NC_008611.1 1725487 1726764 D catalyzes the formation of L-tryptophan from L-serine and 1-(indol-3-yl)glycerol 3-phosphate; tryptophan synthase subunit beta 1725487..1726764 Mycobacterium ulcerans Agy99 4552115 YP_905570.1 CDS trpA NC_008611.1 1726767 1727576 D catalyzes the formation of indole and glyceraldehyde 3-phosphate from indoleglycerol phosphate in tryptophan biosynthesis; tryptophan synthase subunit alpha 1726767..1727576 Mycobacterium ulcerans Agy99 4552116 YP_905571.1 CDS MUL_1595 NC_008611.1 1729539 1729931 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 1729539..1729931 Mycobacterium ulcerans Agy99 4552118 YP_905572.1 CDS MUL_1596 NC_008611.1 1729921 1730364 D membrane protein; hypothetical protein 1729921..1730364 Mycobacterium ulcerans Agy99 4552119 YP_905573.1 CDS cut1 NC_008611.1 1730386 1731054 R secreted protein; hydrolysis of cutin.; cutinase Cut1 complement(1730386..1731054) Mycobacterium ulcerans Agy99 4552120 YP_905574.1 CDS pykA NC_008611.1 1731548 1732966 D catalyzes the formation of phosphoenolpyruvate from pyruvate; pyruvate kinase 1731548..1732966 Mycobacterium ulcerans Agy99 4552121 YP_905575.1 CDS tesB1 NC_008611.1 1733090 1733971 D cytoplasmic protein; involved in fatty acid metabolism.; acyl-CoA thioesterase II TesB1 1733090..1733971 Mycobacterium ulcerans Agy99 4552122 YP_905576.1 CDS MUL_1600 NC_008611.1 1734103 1735503 D membrane protein; hypothetical protein 1734103..1735503 Mycobacterium ulcerans Agy99 4552123 YP_905577.1 CDS cydC NC_008611.1 1735465 1737120 R secreted protein; involved in active transport across the membrane of component linked with the assembly of cytochrome: involved in cytochrome biogenesis (aerobic respiration) responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane.; component linked with the assembly of cytochrome transport transmembrane ATP-binding protein ABC transporter CydC complement(1735465..1737120) Mycobacterium ulcerans Agy99 4552124 YP_905578.1 CDS cydD NC_008611.1 1737146 1738744 R secreted protein; involved in active transport across the membrane of component linked with the assembly of cytochrome: involved in cytochrome biogenesis (aerobic respiration) responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane.; component linked with the assembly of cytochrome transport transmembrane ATP-binding protein ABC transporter CydD complement(1737146..1738744) Mycobacterium ulcerans Agy99 4552125 YP_905579.1 CDS cydB NC_008611.1 1738257 1739873 R membrane protein; involved in the respiratory chain (at the terminal step): aerobic respiration. cytochrome D terminal oxidase complex is the component of the aerobic respiratory chain that is supposed predominated when cells are grown at low aeration [catalytic activity: ubiquinol-8 + O(2) = ubiquinone-8 + H(2)O]; integral membrane cytochrome D ubiquinol oxidase (subunit II) CydB complement(1738257..1739873) Mycobacterium ulcerans Agy99 4552126 YP_905580.1 CDS MUL_1605 NC_008611.1 1741482 1742060 R membrane protein; hypothetical protein complement(1741482..1742060) Mycobacterium ulcerans Agy99 4552091 YP_905581.1 CDS MUL_1608 NC_008611.1 1743757 1744383 D Also detected in the extracellular matrix and the membrane fractions by proteomics.; cytoplasmic protein; sensor part of a two component regulatory system; two-component system transcriptional regulator 1743757..1744383 Mycobacterium ulcerans Agy99 4552094 YP_905582.1 CDS MUL_1610 NC_008611.1 1745631 1746071 R cytoplasmic protein; hypothetical protein complement(1745631..1746071) Mycobacterium ulcerans Agy99 4552096 YP_905583.1 CDS polA NC_008611.1 1746175 1748874 D has 3'-5' exonuclease, 5'-3' exonuclease and 5'-3'polymerase activities, primarily functions to fill gaps during DNA replication and repair; DNA polymerase I 1746175..1748874 Mycobacterium ulcerans Agy99 4552097 YP_905584.1 CDS rpsA NC_008611.1 1749255 1750700 D in Escherichia coli this protein is involved in binding to the leader sequence of mRNAs and is itself bound to the 30S subunit; autoregulates expression via a C-terminal domain; in most gram negative organisms this protein is composed of 6 repeats of the S1 domain while in gram positive there are 4 repeats; the S1 nucleic acid-binding domain is found associated with other proteins; 30S ribosomal protein S1 1749255..1750700 Mycobacterium ulcerans Agy99 4552098 YP_905585.1 CDS coaE NC_008611.1 1750713 1751936 D catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A; involved in coenzyme A biosynthesis; in Mycobacterium tuberculosis the C-terminal UPF0157 domain appears to be necessary for proper folding of the N-terminal domain; dephospho-CoA kinase/protein folding accessory domain-containing protein 1750713..1751936 Mycobacterium ulcerans Agy99 4552099 YP_905586.1 CDS MUL_1614 NC_008611.1 1752054 1752605 R cytoplasmic protein; hypothetical protein complement(1752054..1752605) Mycobacterium ulcerans Agy99 4552100 YP_905587.1 CDS MUL_1615 NC_008611.1 1752651 1753091 R cytoplasmic protein; hypothetical protein complement(1752651..1753091) Mycobacterium ulcerans Agy99 4552101 YP_905588.1 CDS uvrB NC_008611.1 1753352 1755457 D The UvrABC repair system catalyzes the recognition and processing of DNA lesions. The beta-hairpin of the Uvr-B subunit is inserted between the strands, where it probes for the presence of a lesion; excinuclease ABC subunit B 1753352..1755457 Mycobacterium ulcerans Agy99 4552102 YP_905589.1 CDS MUL_1617 NC_008611.1 1755494 1756906 D membrane protein; thought to be involved in transport of drug across the membrane (export) drug resistance by an export mechanism (conferes resistance to toxic compounds by removing them for the cells); drug efflux membrane protein 1755494..1756906 Mycobacterium ulcerans Agy99 4552103 YP_905590.1 CDS MUL_1618 NC_008611.1 1756895 1758463 R membrane protein; transmembrane protein complement(1756895..1758463) Mycobacterium ulcerans Agy99 4552104 YP_905591.1 CDS MUL_1619 NC_008611.1 1758785 1759225 D Also detected in the extracellular matrix by proteomics.; cytoplasmic protein; function unknown, domain identity to universal stress protein family and to iron-regulated conserved hypothetical protein TB15.3 from M. tuberculosis H37Rv.; iron-regulated protein 1758785..1759225 Mycobacterium ulcerans Agy99 4552105 YP_905592.1 CDS MUL_1620 NC_008611.1 1759594 1759986 D Also detected in the extracellular matrix by proteomics.; cytoplasmic protein; hypothetical protein 1759594..1759986 Mycobacterium ulcerans Agy99 4552106 YP_905593.1 CDS MUL_1621 NC_008611.1 1760106 1760783 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(1760106..1760783) Mycobacterium ulcerans Agy99 4552107 YP_905594.1 CDS uvrA NC_008611.1 1760979 1763894 D The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by uvrB, the uvrA molecules dissociate; excinuclease ABC subunit A 1760979..1763894 Mycobacterium ulcerans Agy99 4552071 YP_905595.1 CDS MUL_1624 NC_008611.1 1765618 1765920 R cytoplasmic protein; hypothetical protein complement(1765618..1765920) Mycobacterium ulcerans Agy99 4552073 YP_905596.1 CDS MUL_1625 NC_008611.1 1766008 1767453 R membrane protein; hypothetical protein complement(1766008..1767453) Mycobacterium ulcerans Agy99 4552074 YP_905597.1 CDS lysS NC_008611.1 1767497 1770790 R catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; lysyl-tRNA synthetase complement(1767497..1770790) Mycobacterium ulcerans Agy99 4552075 YP_905598.1 CDS infC NC_008611.1 1771295 1771831 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins.; initiation factor IF-3 InfC 1771295..1771831 Mycobacterium ulcerans Agy99 4552076 YP_905599.1 CDS rpmI NC_008611.1 1771862 1772056 D cytoplasmic protein; translation; 50S ribosomal protein L35 1771862..1772056 Mycobacterium ulcerans Agy99 4552077 YP_905600.1 CDS rplT NC_008611.1 1772127 1772516 D binds directly to 23S ribosomal RNA prior to in vitro assembly of the 50S ribosomal subunit; 50S ribosomal protein L20 1772127..1772516 Mycobacterium ulcerans Agy99 4552078 YP_905601.1 CDS tsnR NC_008611.1 1772594 1773421 D cytoplasmic protein; rRNA modification; 23S rRNA methyltransferase TsnR 1772594..1773421 Mycobacterium ulcerans Agy99 4552079 YP_905602.1 CDS MUL_1631 NC_008611.1 1773425 1774465 R cytoplasmic protein; hypothetical protein complement(1773425..1774465) Mycobacterium ulcerans Agy99 4552080 YP_905603.1 CDS MUL_1632 NC_008611.1 1774819 1775760 D membrane protein; PE family protein 1774819..1775760 Mycobacterium ulcerans Agy99 4552081 YP_905604.1 CDS MUL_1633 NC_008611.1 1775871 1776782 D cytoplasmic protein; hypothetical protein 1775871..1776782 Mycobacterium ulcerans Agy99 4552082 YP_905605.1 CDS MUL_1634 NC_008611.1 1776792 1777574 D membrane protein; transmembrane protein 1776792..1777574 Mycobacterium ulcerans Agy99 4552083 YP_905606.1 CDS pheS NC_008611.1 1777832 1778875 D catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a heterotetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 1 subfamily; phenylalanyl-tRNA synthetase subunit alpha 1777832..1778875 Mycobacterium ulcerans Agy99 4552084 YP_905607.1 CDS pheT NC_008611.1 1778875 1781364 D catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a tetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 2 subfamily; phenylalanyl-tRNA synthetase subunit beta 1778875..1781364 Mycobacterium ulcerans Agy99 4552085 YP_905608.1 CDS MUL_1638 NC_008611.1 1782645 1783691 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 1782645..1783691 Mycobacterium ulcerans Agy99 4552087 YP_905609.1 CDS MUL_1639 NC_008611.1 1785150 1786820 D membrane protein; function unknown.; PE-PGRS family protein 1785150..1786820 Mycobacterium ulcerans Agy99 4552088 YP_905610.1 CDS MUL_1640 NC_008611.1 1787077 1788921 D membrane protein; function unknown.; PE-PGRS family protein 1787077..1788921 Mycobacterium ulcerans Agy99 4552089 YP_905611.1 CDS MUL_1642 NC_008611.1 1790352 1791398 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 1790352..1791398 Mycobacterium ulcerans Agy99 4552055 YP_905612.1 CDS MUL_1643 NC_008611.1 1792173 1793507 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(1792173..1793507) Mycobacterium ulcerans Agy99 4552056 YP_905613.1 CDS argC NC_008611.1 1795252 1796304 D catalyzes the reduction of N-acetyl-5-glutamyl phosphate to N-acetyl-L-glutamate 5-semialdehyde in arginine biosynthesis and the reduction of N-acetyl-gamma-aminoadipyl-phosphate to N-acetyl-L-aminoadipate-semialdehyde in lysine biosynthesis; involved in both the arginine and lysine biosynthetic pathways; lysine is produced via the AAA pathway, lysine from alpha-aminoadipate; N-acetyl-gamma-glutamyl-phosphate reductase 1795252..1796304 Mycobacterium ulcerans Agy99 4552057 YP_905614.1 CDS argJ NC_008611.1 1796377 1797591 D bifunctional arginine biosynthesis protein ArgJ; functions at the 1st and 5th steps in arginine biosynthesis; involved in synthesis of acetylglutamate from glutamate and acetyl-CoA and ornithine by transacetylation between acetylornithine and glutmate; bifunctional ornithine acetyltransferase/N-acetylglutamate synthase 1796377..1797591 Mycobacterium ulcerans Agy99 4552058 YP_905615.1 CDS argB NC_008611.1 1797588 1798469 D catalyzes the phosphorylation of N-acetyl-L-glutamate to form N-acetyl-L-glutamate 5-phosphate; acetylglutamate kinase 1797588..1798469 Mycobacterium ulcerans Agy99 4552059 YP_905616.1 CDS argD NC_008611.1 1798466 1799656 D catalyzes the formation of N-acetyl-l-glutamate 5-semialdehyde from 2-oxoglutarate and N(2)-acetyl-L-ornithine; acetylornithine aminotransferase 1798466..1799656 Mycobacterium ulcerans Agy99 4552060 YP_905617.1 CDS argF NC_008611.1 1799653 1800579 D catalyzes the formation of L-citrulline from carbamoyl phosphate and L-ornithine in arginine biosynthesis and degradation; ornithine carbamoyltransferase 1799653..1800579 Mycobacterium ulcerans Agy99 4552061 YP_905618.1 CDS argR NC_008611.1 1800576 1801076 D regulates arginine biosynthesis when complexed with arginine by binding at site that overlap the promotors of the arginine biosynthesis genes; arginine repressor 1800576..1801076 Mycobacterium ulcerans Agy99 4552062 YP_905619.1 CDS argG NC_008611.1 1801125 1802321 D catalyzes the formation of 2-N(omega)-(L-arginino)succinate from L-citrulline and L-aspartate in arginine biosynthesis, AMP-forming; argininosuccinate synthase 1801125..1802321 Mycobacterium ulcerans Agy99 4552063 YP_905620.1 CDS argH NC_008611.1 1802416 1803828 D catalyzes the formation of arginine from (N-L-arginino)succinate; argininosuccinate lyase 1802416..1803828 Mycobacterium ulcerans Agy99 4552064 YP_905621.1 CDS pks9 NC_008611.1 1817861 1820953 D membrane protein; polyketide synthase Pks9 1817861..1820953 Mycobacterium ulcerans Agy99 4552068 YP_905622.1 CDS pks11 NC_008611.1 1821108 1822169 D membrane protein; possibly involved in the biosynthesis of secondary metabolites [catalytic activity: 3 malonyl-CoA + 4- coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2]; chalcone synthase, Pks11 1821108..1822169 Mycobacterium ulcerans Agy99 4552069 YP_905623.1 CDS MUL_1658 NC_008611.1 1823233 1825215 R cytoplasmic protein; thought to be involved in active transport of macrolide across the membrane (export) macrolide antibiotics resistance by an export mechanism. responsible for energy coupling to the transport system; ABC transporter ATP-binding protein complement(1823233..1825215) Mycobacterium ulcerans Agy99 4551930 YP_905624.1 CDS aao_1 NC_008611.1 1826160 1827272 D membrane protein; wide specificity for D-amino acids. also acts on glycine [catalytic activity: a D-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2]; D-amino acid oxidase Aao 1826160..1827272 Mycobacterium ulcerans Agy99 4551931 YP_905625.1 CDS MUL_1660 NC_008611.1 1827419 1830424 D activates fatty acids by binding to coenzyme A; acyl-CoA synthetase 1827419..1830424 Mycobacterium ulcerans Agy99 4551932 YP_905626.1 CDS MUL_1661 NC_008611.1 1830417 1830650 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein 1830417..1830650 Mycobacterium ulcerans Agy99 4551933 YP_905627.1 CDS gloA NC_008611.1 1830751 1831197 D cytoplasmic protein; role in amino acid transport and metabolism; lactoylglutathione lyase, GloA 1830751..1831197 Mycobacterium ulcerans Agy99 4551934 YP_905628.1 CDS MUL_1664 NC_008611.1 1833833 1834432 R cytoplasmic protein; unknwon; hypothetical protein complement(1833833..1834432) Mycobacterium ulcerans Agy99 4551936 YP_905629.1 CDS MUL_1665 NC_008611.1 1834434 1835114 R membrane protein; thought to be involved in active transport of undeterminated substrate (possibly drug) across the membrane. responsible for the translocation of the substrate across the membrane; antibiotic resistance ABC transporter efflux protein complement(1834434..1835114) Mycobacterium ulcerans Agy99 4551937 YP_905630.1 CDS MUL_1666 NC_008611.1 1835186 1835953 R membrane protein; thought to be involved in active transport of undeterminated substrate (possibly drug) across the membrane. responsible for energy coupling to the transport system; antibiotic resistance ABC transporter efflux system, ATP-binding protein complement(1835186..1835953) Mycobacterium ulcerans Agy99 4551938 YP_905631.1 CDS mpg NC_008611.1 1836013 1836624 D responsible for recognizing base lesions in the genome and initiating base excision DNA repair; 3-methyladenine DNA glycosylase 1836013..1836624 Mycobacterium ulcerans Agy99 4551939 YP_905632.1 CDS tyrS NC_008611.1 1836636 1837928 D catalyzes the formation of tyrosyl-tRNA(Tyr) from tyrosine and tRNA(Tyr); tyrosyl-tRNA synthetase 1836636..1837928 Mycobacterium ulcerans Agy99 4551940 YP_905633.1 CDS MUL_1669 NC_008611.1 1838234 1838524 D cytoplasmic protein; function unknown. no blast similarities.; hypothetical protein 1838234..1838524 Mycobacterium ulcerans Agy99 4551941 YP_905634.1 CDS MUL_1670 NC_008611.1 1838767 1839084 R cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 complement(1838767..1839084) Mycobacterium ulcerans Agy99 4551942 YP_905635.1 CDS MUL_1671 NC_008611.1 1839109 1839813 R cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 complement(1839109..1839813) Mycobacterium ulcerans Agy99 4551943 YP_905636.1 CDS MUL_1674 NC_008611.1 1841822 1843768 R membrane protein; hypothetical protein complement(1841822..1843768) Mycobacterium ulcerans Agy99 4551945 YP_905637.1 CDS MUL_1676 NC_008611.1 1845469 1846515 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase from IS2404 complement(1845469..1846515) Mycobacterium ulcerans Agy99 4551947 YP_905638.1 CDS MUL_1677 NC_008611.1 1847317 1848810 R cytoplasmic protein; hypothetical protein complement(1847317..1848810) Mycobacterium ulcerans Agy99 4551911 YP_905639.1 CDS lprJ NC_008611.1 1850626 1851015 D membrane protein; lipoprotein LprJ 1850626..1851015 Mycobacterium ulcerans Agy99 4551913 YP_905640.1 CDS MUL_1680 NC_008611.1 1851076 1851909 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein 1851076..1851909 Mycobacterium ulcerans Agy99 4551914 YP_905641.1 CDS MUL_1681 NC_008611.1 1851912 1852922 D cytoplasmic protein; function unknown; probably involved in cellular metabolism; phosphatase 1851912..1852922 Mycobacterium ulcerans Agy99 4551915 YP_905642.1 CDS MUL_1682 NC_008611.1 1852947 1853123 D cytoplasmic protein; hypothetical protein 1852947..1853123 Mycobacterium ulcerans Agy99 4551916 YP_905643.1 CDS tlyA NC_008611.1 1853131 1853976 D cytoplasmic protein; has a contact-dependent haemolytic activity; possibly involved in virulence (pore formation); cytotoxin/hemolysin, TlyA 1853131..1853976 Mycobacterium ulcerans Agy99 4551917 YP_905644.1 CDS ppnK NC_008611.1 1853973 1854896 D catalyzes the phosphorylation of NAD to NADP; inorganic polyphosphate/ATP-NAD kinase 1853973..1854896 Mycobacterium ulcerans Agy99 4551918 YP_905645.1 CDS recN NC_008611.1 1854909 1856654 D cytoplasmic protein; involved in recombinational repair of damaged DNA.; DNA repair protein RecN 1854909..1856654 Mycobacterium ulcerans Agy99 4551919 YP_905646.1 CDS MUL_1686 NC_008611.1 1856820 1858001 D membrane protein; hypothetical protein 1856820..1858001 Mycobacterium ulcerans Agy99 4551920 YP_905647.1 CDS MUL_1687 NC_008611.1 1858023 1858976 D secreted protein; hypothetical protein 1858023..1858976 Mycobacterium ulcerans Agy99 4551921 YP_905648.1 CDS pyrG NC_008611.1 1859114 1860865 D CTP synthase; cytidine triphosphate synthetase; catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; in Escherichia coli this enzyme forms a homotetramer; CTP synthetase 1859114..1860865 Mycobacterium ulcerans Agy99 4551922 YP_905649.1 CDS MUL_1689 NC_008611.1 1860858 1861481 D cytoplasmic protein; NUDIX hydrolases catalyze the hydrolysis of a variety of nucleoside diphosphate derivatives; NUDIX hydrolase 1860858..1861481 Mycobacterium ulcerans Agy99 4551923 YP_905650.1 CDS xerD NC_008611.1 1861478 1862419 D site-specific tyrosine recombinase which cuts and rejoins DNA molecules; binds cooperatively to specific DNA consensus sites; forms a heterotetrameric complex with XerC; XerCD exhibit similar sequences; essential to convert chromosome dimers to monomers during cell division and functions during plasmid segregation; XerD specifically exchanges the bottom strands; cell division protein FtsK may regulate the XerCD complex; enzyme from Streptococcus group has unusual active site motifs; site-specific tyrosine recombinase XerD 1861478..1862419 Mycobacterium ulcerans Agy99 4551924 YP_905651.1 CDS MUL_1691 NC_008611.1 1862426 1863019 R cytoplasmic protein; catalyzes the O-methylation [catalytic activity: S- adenosyl-L-methionine + catechol = S-adenosyl-L- homocysteine + guaiacol]; O-methyltransferase complement(1862426..1863019) Mycobacterium ulcerans Agy99 4551925 YP_905652.1 CDS MUL_1692 NC_008611.1 1863338 1864561 D membrane protein; this is a family of myo-inositol-1-phosphate synthases. inositol-1-phosphate catalyses the conversion of glucose-6- phosphate to inositol-1-phosphate, which is then dephosphorylated to inositol. inositol phosphates play an important role in signal transduction.; myo-inositol-1-phosphate synthase 1863338..1864561 Mycobacterium ulcerans Agy99 4551926 YP_905653.1 CDS MUL_1693 NC_008611.1 1864558 1865421 D membrane protein; membrane associated. contains Cdd matches to polyprenyltransferase domains.; transferase 1864558..1865421 Mycobacterium ulcerans Agy99 4551927 YP_905654.1 CDS MUL_1694 NC_008611.1 1865421 1866842 D cytoplasmic protein; contains Cdd COG1082,sugar phosphate isomerases/epimerases [carbohydrate transport and metabolism; sugar phosphate isomerases/epimerases 1865421..1866842 Mycobacterium ulcerans Agy99 4551928 YP_905655.1 CDS MUL_1695 NC_008611.1 1866839 1867690 D cytoplasmic protein; contains Cdd COG1099, predicted metal-dependent hydrolases with the TIM-barrel FolD.; metal dependent hydrolase 1866839..1867690 Mycobacterium ulcerans Agy99 4551929 YP_905656.1 CDS MUL_1697 NC_008611.1 1868798 1870177 D membrane protein; function unknown.; hypothetical protein 1868798..1870177 Mycobacterium ulcerans Agy99 4551878 YP_905657.1 CDS MUL_1698 NC_008611.1 1870323 1871297 D cytoplasmic protein; hypothetical protein 1870323..1871297 Mycobacterium ulcerans Agy99 4551879 YP_905658.1 CDS MUL_1699 NC_008611.1 1871298 1872344 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(1871298..1872344) Mycobacterium ulcerans Agy99 4551880 YP_905659.1 CDS MUL_1701 NC_008611.1 1874423 1875601 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; PPE family protein complement(1874423..1875601) Mycobacterium ulcerans Agy99 4551882 YP_905660.1 CDS MUL_1702 NC_008611.1 1875833 1876963 R PPE23; membrane protein; PPE family protein complement(1875833..1876963) Mycobacterium ulcerans Agy99 4551883 YP_905661.1 CDS mbtH_2 NC_008611.1 1881575 1881799 R cytoplasmic protein; thought to be involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins; protein MbtH_2 complement(1881575..1881799) Mycobacterium ulcerans Agy99 4551885 YP_905662.1 CDS MUL_1705 NC_008611.1 1881796 1883049 R cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase complement(1881796..1883049) Mycobacterium ulcerans Agy99 4551886 YP_905663.1 CDS MUL_1706 NC_008611.1 1883056 1883310 R cytoplasmic protein; hypothetical protein complement(1883056..1883310) Mycobacterium ulcerans Agy99 4551887 YP_905664.1 CDS MUL_1707 NC_008611.1 1883463 1884509 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(1883463..1884509) Mycobacterium ulcerans Agy99 4551888 YP_905665.1 CDS speE NC_008611.1 1884789 1886375 R catalyzes the formation of spermidine from putrescine and S-adenosylmethioninamine; spermidine synthase complement(1884789..1886375) Mycobacterium ulcerans Agy99 4551889 YP_905666.1 CDS MUL_1709 NC_008611.1 1886372 1886842 R membrane protein; hypothetical protein complement(1886372..1886842) Mycobacterium ulcerans Agy99 4551890 YP_905667.1 CDS MUL_1710 NC_008611.1 1886876 1887256 R membrane protein; function unknown, possible membrane protein; hypothetical protein complement(1886876..1887256) Mycobacterium ulcerans Agy99 4551891 YP_905668.1 CDS MUL_1711 NC_008611.1 1887559 1888605 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 1887559..1888605 Mycobacterium ulcerans Agy99 4551892 YP_905669.1 CDS MUL_1712 NC_008611.1 1888680 1889192 R membrane protein; hypothetical protein complement(1888680..1889192) Mycobacterium ulcerans Agy99 4551893 YP_905670.1 CDS MUL_1713 NC_008611.1 1889202 1889810 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(1889202..1889810) Mycobacterium ulcerans Agy99 4551862 YP_905671.1 CDS ruvC NC_008611.1 1890025 1890579 D endonuclease; resolves Holliday structures; forms a complex of RuvABC; the junction binding protein RuvA forms a hexameric ring along with the RuvB helicase and catalyzes branch migration; RuvC then interacts with RuvAB to resolve the Holliday junction by nicking DNA strands of like polarity; Holliday junction resolvase 1890025..1890579 Mycobacterium ulcerans Agy99 4551863 YP_905672.1 CDS ruvA NC_008611.1 1890576 1891169 D plays an essential role in ATP-dependent branch migration of the Holliday junction; Holliday junction DNA helicase RuvA 1890576..1891169 Mycobacterium ulcerans Agy99 4551864 YP_905673.1 CDS ruvB NC_008611.1 1891173 1892210 D promotes strand exchange during homologous recombination; RuvAB complex promotes branch migration; RuvABC complex scans the DNA during branch migration and resolves Holliday junctions at consensus sequences; forms hexameric rings around opposite DNA arms; requires ATP for branch migration and orientation of RuvAB complex determines direction of migration; Holliday junction DNA helicase RuvB 1891173..1892210 Mycobacterium ulcerans Agy99 4551865 YP_905674.1 CDS MUL_1718 NC_008611.1 1894101 1895549 R membrane protein; PE-PGRS family protein complement(1894101..1895549) Mycobacterium ulcerans Agy99 4551867 YP_905675.1 CDS MUL_1719 NC_008611.1 1895741 1896130 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown, domain identity membrane protein Cdd COG3759; hypothetical protein 1895741..1896130 Mycobacterium ulcerans Agy99 4551868 YP_905676.1 CDS MUL_1720 NC_008611.1 1896220 1896912 D secreted protein; hypothetical protein 1896220..1896912 Mycobacterium ulcerans Agy99 4551869 YP_905677.1 CDS MUL_1721 NC_008611.1 1897225 1898610 R membrane protein; PE PGRS family protein complement(1897225..1898610) Mycobacterium ulcerans Agy99 4551870 YP_905678.1 CDS MUL_1722 NC_008611.1 1898764 1902288 R cytoplasmic protein; function unknown, but involved in lipid degradation.; fatty-acid-CoA ligase FadD9 complement(1898764..1902288) Mycobacterium ulcerans Agy99 4551871 YP_905679.1 CDS gabT NC_008611.1 1902469 1903809 R catalyzes the formation of succinate semialdehyde and glutamate from 4-aminobutanoate and 2-oxoglutarate; 4-aminobutyrate aminotransferase complement(1902469..1903809) Mycobacterium ulcerans Agy99 4551872 YP_905680.1 CDS yajC NC_008611.1 1904039 1904380 D member of preprotein translocase; forms a heterotrimer with SecD and SecF; links the SecD/SecF/YajC/YidC complex with the SecY/SecE/SecG complex; preprotein translocase subunit YajC 1904039..1904380 Mycobacterium ulcerans Agy99 4551873 YP_905681.1 CDS secD NC_008611.1 1904493 1906340 D part of the preprotein secretory system; when complexed with proteins SecF and YajC, SecDFyajC stimulates the proton motive force-driven protein translocation, and appears to be required for the release of mature proteins from the extracytoplasmic side of the membrane; preprotein translocase subunit SecD 1904493..1906340 Mycobacterium ulcerans Agy99 4551874 YP_905682.1 CDS secF NC_008611.1 1906345 1907685 D forms a complex with SecD and YajC; SecDFyajC stimulates the proton motive force-driven protein translocation; seems to modulate the cycling of SecA by stabilizing its membrane-inserted state and appears to be required for the release of mature proteins from the extracytoplasmic side of the membrane; in some organisms, such as Bacillus subtilis, SecD is fused to SecF; preprotein translocase subunit SecF 1906345..1907685 Mycobacterium ulcerans Agy99 4551875 YP_905683.1 CDS MUL_1727 NC_008611.1 1907691 1909403 D secreted protein; function unknown. possible COG0747, DdpA, ABC-type dipeptide transport system, periplasmic component [amino acid transport and metabolism]; dipeptide ABC transporter periplasmic protein 1907691..1909403 Mycobacterium ulcerans Agy99 4551876 YP_905684.1 CDS apt NC_008611.1 1909400 1909954 D catalyzes a salvage reaction resulting in the formation of AMP which is metabolically less costly than a de novo synthesis; adenine phosphoribosyltransferase 1909400..1909954 Mycobacterium ulcerans Agy99 4551824 YP_905685.1 CDS relA NC_008611.1 1910022 1912394 D Detected in the cytoplamic fraction by LCMSMS; cytoplasmic protein; involved in the metabolism of PPGPP (at the first step) in eubacteria PPGPP (guanosine 3'-diphosphate 5- 'diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. this enzyme catalyzes the formation of PPPGPP which is then hydrolysed to form PPGPP [catalytic activity: ATP + GTP = AMP + guanosine 3'- diphosphate 5-'triphosphate]; GTP pyrophosphokinase RelA 1910022..1912394 Mycobacterium ulcerans Agy99 4551825 YP_905686.1 CDS ppiB NC_008611.1 1912451 1913428 R Detected in the secreted and membrane fractions by proteomics.; membrane protein; Ppiases accelerate the folding of proteins [catalytic activity: cis-trans isomerization of proline imidic peptide bonds in oligopeptides]; peptidyl-prolyl cis-trans isomerase B PpiB complement(1912451..1913428) Mycobacterium ulcerans Agy99 4551826 YP_905687.1 CDS MUL_1731 NC_008611.1 1913479 1914153 D cytoplasmic protein; involved in glyoxal pathway. thioesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid [catalytic activity: (S)-(2-hydroxyacyl)glutathione + H(2)O = glutathione + a 2- hydroxy acid anion]; Zn-dependent glyoxylase 1913479..1914153 Mycobacterium ulcerans Agy99 4551827 YP_905688.1 CDS hisS NC_008611.1 1914150 1915412 D catalyzes a two-step reaction, first charging a histidine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class II aminoacyl-tRNA synthetase; forms homodimers; some organisms have a paralogous gene, hisZ, that is similar to hisS and produces a protein that performs the first step in histidine biosynthesis along with HisG; histidyl-tRNA synthetase 1914150..1915412 Mycobacterium ulcerans Agy99 4551828 YP_905689.1 CDS MUL_1734 NC_008611.1 1917222 1918250 D May be regulated by LexA; cytoplasmic protein; hypothetical protein 1917222..1918250 Mycobacterium ulcerans Agy99 4551830 YP_905690.1 CDS MUL_1736 NC_008611.1 1919880 1920782 R Contains patatin domain. This family consists of various patatin glycoproteins from plants. The patatin protein accounts for up to 40% of the total soluble protein in potato tubers. Patatin is a storage protein but it also has the enzymatic activity of lipid acyl hydrolase.; cytoplasmic protein; patatin-like phospholipase catalysing the cleavage of fatty acids from membrane lipids.; alpha-beta hydrolase superfamily esterase complement(1919880..1920782) Mycobacterium ulcerans Agy99 4551832 YP_905691.1 CDS MUL_1737 NC_008611.1 1921247 1921789 R cytoplasmic protein; function unknown. contains Cdd pfam01381, HTH_3, helix-turn-helix of DNA binding proteins.; transcriptional regulator complement(1921247..1921789) Mycobacterium ulcerans Agy99 4551833 YP_905692.1 CDS dapA_1 NC_008611.1 1922733 1923623 D cytoplasmic protein; involved in biosynthesis of diaminopimelate and lysine from aspartate semialdehyde (at the first step) [catalytic activity: l-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H(2)O]; dihydrodipicolinate synthase DapA 1922733..1923623 Mycobacterium ulcerans Agy99 4551835 YP_905693.1 CDS MUL_1740 NC_008611.1 1923673 1924557 R membrane protein; membrane glycine rich protein complement(1923673..1924557) Mycobacterium ulcerans Agy99 4551836 YP_905694.1 CDS MUL_1741 NC_008611.1 1924619 1925134 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(1924619..1925134) Mycobacterium ulcerans Agy99 4551837 YP_905695.1 CDS apbA_1 NC_008611.1 1925153 1925893 R ketopantoate reductase; catalyzes the NADPH reduction of ketopantoate to pantoate; functions in pantothenate (vitamin B5) biosynthesis; 2-dehydropantoate 2-reductase complement(1925153..1925893) Mycobacterium ulcerans Agy99 4551838 YP_905696.1 CDS aspS NC_008611.1 1926132 1927913 D catalyzes a two-step reaction, first charging an aspartate molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; contains discriminating and non-discriminating subtypes; aspartyl-tRNA synthetase 1926132..1927913 Mycobacterium ulcerans Agy99 4551839 YP_905697.1 CDS MUL_1745 NC_008611.1 1929243 1929650 R cytoplasmic protein; hypothetical protein complement(1929243..1929650) Mycobacterium ulcerans Agy99 4551842 YP_905698.1 CDS MUL_1746 NC_008611.1 1929718 1930728 D cytoplasmic protein; function unknown, but contains Cdd COG1305, transglutaminase-like enzymes, putative cysteine proteases; hypothetical protein 1929718..1930728 Mycobacterium ulcerans Agy99 4551785 YP_905699.1 CDS MUL_1747 NC_008611.1 1930721 1931776 D cytoplasmic protein; hypothetical protein 1930721..1931776 Mycobacterium ulcerans Agy99 4551786 YP_905700.1 CDS MUL_1748 NC_008611.1 1931800 1934487 R membrane protein; hypothetical protein complement(1931800..1934487) Mycobacterium ulcerans Agy99 4551787 YP_905701.1 CDS MUL_1749 NC_008611.1 1934521 1936188 R cytoplasmic protein; function unknown, in general transglutaminases are enzymes that establish covalent links between proteins. a subset of transglutaminase homologues appear to catalyse the reverse reaction, the hydrolysis of peptide bonds.; transglutaminase family protein complement(1934521..1936188) Mycobacterium ulcerans Agy99 4551788 YP_905702.1 CDS MUL_1750 NC_008611.1 1936324 1937430 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; proline and glycine rich transmembrane protein complement(1936324..1937430) Mycobacterium ulcerans Agy99 4551789 YP_905703.1 CDS MUL_1751 NC_008611.1 1937621 1938979 D cytoplasmic protein; recombination factor protein RarA 1937621..1938979 Mycobacterium ulcerans Agy99 4551790 YP_905704.1 CDS MUL_1752 NC_008611.1 1939025 1939846 R cytoplasmic protein; function unknown, thought to be involved in the persistence in the host.; hypothetical protein complement(1939025..1939846) Mycobacterium ulcerans Agy99 4551791 YP_905705.1 CDS MUL_1753 NC_008611.1 1939862 1940263 D cytoplasmic protein; hypothetical protein 1939862..1940263 Mycobacterium ulcerans Agy99 4551792 YP_905706.1 CDS alaS NC_008611.1 1940382 1943087 D Catalyzes a two-step reaction, first charging an alanyl molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; alanyl-tRNA synthetase 1940382..1943087 Mycobacterium ulcerans Agy99 4551793 YP_905707.1 CDS MUL_1755 NC_008611.1 1943088 1943600 D similar to RuvC resolvase with substantial differences; NMR structural information suggests this protein is monomeric; unknown cellular function; Holliday junction resolvase-like protein 1943088..1943600 Mycobacterium ulcerans Agy99 4551794 YP_905708.1 CDS MUL_1756 NC_008611.1 1943593 1944849 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 1943593..1944849 Mycobacterium ulcerans Agy99 4551795 YP_905709.1 CDS aroE NC_008611.1 1944846 1945679 D AroE; catalyzes the conversion of shikimate to 3-dehydroshikimate; shikimate 5-dehydrogenase 1944846..1945679 Mycobacterium ulcerans Agy99 4551796 YP_905710.1 CDS aroF NC_008611.1 1946212 1947435 D catalyzes the formation of chorismate from 5-O-(1-carboxyvinyl)-3-phosphoshikimate in aromatic amino acid biosynthesis; chorismate synthase 1946212..1947435 Mycobacterium ulcerans Agy99 4551798 YP_905711.1 CDS aroK NC_008611.1 1947444 1947998 D catalyzes the formation of shikimate 3-phosphate from shikimate in aromatic amino acid biosynthesis; shikimate kinase 1947444..1947998 Mycobacterium ulcerans Agy99 4551799 YP_905712.1 CDS aroB NC_008611.1 1947995 1949083 D catalyzes the formation of 3-dehydroquinate from 3-deoxy-arabino-heptulonate 7-phosphate; functions in aromatic amino acid biosynthesis; 3-dehydroquinate synthase 1947995..1949083 Mycobacterium ulcerans Agy99 4551800 YP_905713.1 CDS aroD NC_008611.1 1949080 1949520 D catalyzes the formation of 3-dehydroshikimate from 3-dehydroquinate in chorismate biosynthesis; 3-dehydroquinate dehydratase 1949080..1949520 Mycobacterium ulcerans Agy99 4551801 YP_905714.1 CDS MUL_1763 NC_008611.1 1949517 1950275 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; transmembrane protein complement(1949517..1950275) Mycobacterium ulcerans Agy99 4551802 YP_905715.1 CDS pepQ NC_008611.1 1950309 1951427 D cytoplasmic protein; function unknown, hydrolyses peptides.; cytoplasmic peptidase PepQ 1950309..1951427 Mycobacterium ulcerans Agy99 4551803 YP_905716.1 CDS efp NC_008611.1 1951442 1952005 D Involved in peptide bond synthesis; alters the affinity of the ribosome for aminoacyl-tRNA; elongation factor P 1951442..1952005 Mycobacterium ulcerans Agy99 4551804 YP_905717.1 CDS nusB NC_008611.1 1952008 1952529 D Regulates rRNA biosynthesis by transcriptional antitermination; transcription antitermination protein NusB 1952008..1952529 Mycobacterium ulcerans Agy99 4551730 YP_905718.1 CDS MUL_1767 NC_008611.1 1952523 1952936 D secreted protein; hypothetical protein 1952523..1952936 Mycobacterium ulcerans Agy99 4551731 YP_905719.1 CDS MUL_1770 NC_008611.1 1955954 1957174 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase complement(1955954..1957174) Mycobacterium ulcerans Agy99 4551733 YP_905720.1 CDS MUL_1771 NC_008611.1 1957220 1957792 R cytoplasmic protein; hypothetical protein complement(1957220..1957792) Mycobacterium ulcerans Agy99 4551734 YP_905721.1 CDS MUL_1772 NC_008611.1 1957808 1959130 R cytoplasmic protein; function unknown.; beta-lactamase complement(1957808..1959130) Mycobacterium ulcerans Agy99 4551735 YP_905722.1 CDS lprF NC_008611.1 1959260 1960300 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; lipoprotein LprF 1959260..1960300 Mycobacterium ulcerans Agy99 4551736 YP_905723.1 CDS MUL_1774 NC_008611.1 1960348 1962030 D membrane protein; hypothetical protein 1960348..1962030 Mycobacterium ulcerans Agy99 4551737 YP_905724.1 CDS MUL_1775 NC_008611.1 1962027 1963208 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; chalcone/stilbene synthase 1962027..1963208 Mycobacterium ulcerans Agy99 4551738 YP_905725.1 CDS MUL_1776 NC_008611.1 1963268 1964500 D cytoplasmic protein; involved in sulfation: activity towards typical ceramide glycolipids and trehalose glycolipids.; glycolipid sulfotransferase 1963268..1964500 Mycobacterium ulcerans Agy99 4551739 YP_905726.1 CDS MUL_1777 NC_008611.1 1964382 1965314 D cytoplasmic protein; involved in sulfation: activity towards typical ceramide glycolipids and trehalose glycolipids.; glycolipid sulfotransferase 1964382..1965314 Mycobacterium ulcerans Agy99 4551740 YP_905727.1 CDS pyrR NC_008611.1 1966139 1966702 D regulates pyrimidine biosynthesis by binding to the mRNA of the pyr genes, also has been shown to have uracil phosphoribosyltransferase activity; bifunctional pyrimidine regulatory protein PyrR/uracil phosphoribosyltransferase 1966139..1966702 Mycobacterium ulcerans Agy99 4551742 YP_905728.1 CDS pyrB NC_008611.1 1966699 1967661 D catalyzes the transfer of the carbamoyl moiety from carbamoyl phosphate to L- aspartate in pyrimidine biosynthesis; aspartate carbamoyltransferase catalytic subunit 1966699..1967661 Mycobacterium ulcerans Agy99 4551743 YP_905729.1 CDS pyrC NC_008611.1 1967658 1968950 D catalyzes the formation of N-carbamoyl-L-aspartate from (S)-dihydroorotate in pyrimidine biosynthesis; dihydroorotase 1967658..1968950 Mycobacterium ulcerans Agy99 4551744 YP_905730.1 CDS MUL_1782 NC_008611.1 1968947 1969456 D secreted protein 1968947..1969456 Mycobacterium ulcerans Agy99 4551745 YP_905731.1 CDS carA NC_008611.1 1969453 1970580 D catalyzes production of carbamoyl phosphate from bicarbonate and glutamine in pyrimidine and arginine biosynthesis pathways; forms an octamer composed of four CarAB dimers; carbamoyl phosphate synthase small subunit 1969453..1970580 Mycobacterium ulcerans Agy99 4551746 YP_905732.1 CDS carB NC_008611.1 1970672 1974037 D four CarB-CarA dimers form the carbamoyl phosphate synthetase holoenzyme that catalyzes the production of carbamoyl phosphate; CarB is responsible for the amidotransferase activity; carbamoyl phosphate synthase large subunit 1970672..1974037 Mycobacterium ulcerans Agy99 4551747 YP_905733.1 CDS pyrF NC_008611.1 1974034 1974870 D type 2 subfamily; involved in last step of pyrimidine biosynthesis; converts orotidine 5'-phosphate to UMP and carbon dioxide; OMP decarboxylase; OMPDCase; OMPdecase; orotidine 5'-phosphate decarboxylase 1974034..1974870 Mycobacterium ulcerans Agy99 4551748 YP_905734.1 CDS mihF NC_008611.1 1975835 1976152 D Detected in the cytoplamic fraction by LCMSMS. Also detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; integration host factor MihF 1975835..1976152 Mycobacterium ulcerans Agy99 4551711 YP_905735.1 CDS gmk NC_008611.1 1976249 1976875 D Essential for recycling GMP and indirectly, cGMP; guanylate kinase 1976249..1976875 Mycobacterium ulcerans Agy99 4551712 YP_905736.1 CDS rpoZ NC_008611.1 1976962 1977294 D Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits; DNA-directed RNA polymerase subunit omega 1976962..1977294 Mycobacterium ulcerans Agy99 4551713 YP_905737.1 CDS dfp NC_008611.1 1977310 1978551 D catalyzes the conjugation of cysteine to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, which is then decarboxylated to form 4'-phosphopantotheine; bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase 1977310..1978551 Mycobacterium ulcerans Agy99 4551714 YP_905738.1 CDS metK NC_008611.1 1978680 1979891 D catalyzes the formation of S-adenosylmethionine from methionine and ATP; methionine adenosyltransferase; S-adenosylmethionine synthetase 1978680..1979891 Mycobacterium ulcerans Agy99 4551715 YP_905739.1 CDS MUL_1792 NC_008611.1 1979973 1980581 R secreted protein; hypothetical protein complement(1979973..1980581) Mycobacterium ulcerans Agy99 4551716 YP_905740.1 CDS MUL_1793 NC_008611.1 1980641 1982098 R cytoplasmic protein; function unknown, probably involved in cellular metabolism; monoxygenase complement(1980641..1982098) Mycobacterium ulcerans Agy99 4551717 YP_905741.1 CDS lipH NC_008611.1 1982270 1983241 R cytoplasmic protein; lipase LipH complement(1982270..1983241) Mycobacterium ulcerans Agy99 4551718 YP_905742.1 CDS lipI NC_008611.1 1983268 1984242 R cytoplasmic protein; lipase LipI complement(1983268..1984242) Mycobacterium ulcerans Agy99 4551719 YP_905743.1 CDS MUL_1796 NC_008611.1 1984193 1984978 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; membrane protein 1984193..1984978 Mycobacterium ulcerans Agy99 4551720 YP_905744.1 CDS priA NC_008611.1 1985089 1987035 D binding of PriA to forked DNA starts the assembly of the primosome, also possesses 3'-5' helicase activity; primosome assembly protein PriA 1985089..1987035 Mycobacterium ulcerans Agy99 4551721 YP_905745.1 CDS MUL_1798 NC_008611.1 1987053 1987877 R cytoplasmic protein; causes methylation; methyltransferase complement(1987053..1987877) Mycobacterium ulcerans Agy99 4551722 YP_905746.1 CDS MUL_1799 NC_008611.1 1988114 1989160 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(1988114..1989160) Mycobacterium ulcerans Agy99 4551723 YP_905747.1 CDS MUL_1800 NC_008611.1 1989129 1989959 D cytoplasmic protein; involved in transcriptional mechanism; transcriptional regulatory protein 1989129..1989959 Mycobacterium ulcerans Agy99 4551724 YP_905748.1 CDS MUL_1801 NC_008611.1 1990082 1990906 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; causes methylation; methyltransferase complement(1990082..1990906) Mycobacterium ulcerans Agy99 4551725 YP_905749.1 CDS fmt NC_008611.1 1991108 1992046 D modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet) which is important in translation initiation; inactivation of this gene in Escherichia coli severely impairs growth; methionyl-tRNA formyltransferase 1991108..1992046 Mycobacterium ulcerans Agy99 4551726 YP_905750.1 CDS fmu NC_008611.1 1992115 1993488 D cytoplasmic protein; Fmu protein 1992115..1993488 Mycobacterium ulcerans Agy99 4551727 YP_905751.1 CDS rpe NC_008611.1 1993546 1994244 D catalyzes the interconversion of D-ribulose 5-phosphate to xylulose 5-phosphate; ribulose-phosphate 3-epimerase 1993546..1994244 Mycobacterium ulcerans Agy99 4551728 YP_905752.1 CDS ribG NC_008611.1 1994252 1995271 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; involved in riboflavin biosynthesis (at the second and third steps) converts 2,5-diamino-6-(ribosylamino)- 4(3H)-pyrimidinone 5'-phosphate into 5-amino-6- (ribosylamino)-2,4(1H,3H)-pyrimidinedione 5'-phosphate [catalytic activity 1: 2,5-diamino-6-hydroxy-4-(5- phosphoribosylamino)pyrimidine + H(2)O = 5-amino-6-(5- phosphoribosylamino)uracil + NH(3)] [catalytic activity 2: 5-amino-6-(5-phosphoribitylamino)uracil + NADP(+) = 5- amino-6-(5-phosphoribosylamino)uracil + NADPH]; bifunctional riboflavin biosynthesis protein RibG 1994252..1995271 Mycobacterium ulcerans Agy99 4551729 YP_905753.1 CDS MUL_1806 NC_008611.1 1995276 1996829 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in transport of aminoglycosides and tetracycline across the membrane (export): drug resistance by an export mechanism (conferes resistance to toxic compounds by removing them for the cells) responsible for the translocation of the substrate across the membrane.; aminoglycosides/tetracycline-transport integral membrane protein complement(1995276..1996829) Mycobacterium ulcerans Agy99 4551693 YP_905754.1 CDS lprG NC_008611.1 1996829 1997539 R Detected in the membrane fraction and the cytoplamic fraction by LC.MS/MS.; membrane protein; lipoprotein LprG complement(1996829..1997539) Mycobacterium ulcerans Agy99 4551694 YP_905755.1 CDS ribC NC_008611.1 1997640 1998263 D catalyzes the formation of riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine; riboflavin synthase subunit alpha 1997640..1998263 Mycobacterium ulcerans Agy99 4551695 YP_905756.1 CDS ribA2 NC_008611.1 1998402 1999679 D bifunctional enzyme DHBP synthase/GTP cyclohydrolase II; functions in riboflavin synthesis; converts GTP to 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine; converts ribulose 5-phopshate to 3,4-dihydroxy-2-butanone 4-phosphate; bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II protein 1998402..1999679 Mycobacterium ulcerans Agy99 4551696 YP_905757.1 CDS ribH NC_008611.1 1999676 2000158 D RibE; 6,7-diimethyl-8-ribityllumazine synthase; DMRL synthase; lumazine synthase; beta subunit of riboflavin synthase; condenses 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate to generate 6,6-dimethyl-8-lumazine (DMRL); riboflavin synthase then uses 2 molecules of DMRL to produce riboflavin (vitamin B12); involved in the last steps of riboflavin biosynthesis; forms a 60mer (icosahedral shell) in both Bacillus subtilis and Escherichia coli; in Bacillus subtilis this 60mer is associated with the riboflavin synthase subunit (alpha) while in Escherichia coli it is not; 6,7-dimethyl-8-ribityllumazine synthase 1999676..2000158 Mycobacterium ulcerans Agy99 4551697 YP_905758.1 CDS MUL_1811 NC_008611.1 2000155 2000625 D membrane protein; hypothetical protein 2000155..2000625 Mycobacterium ulcerans Agy99 4551698 YP_905759.1 CDS lprH NC_008611.1 2000675 2001298 D secreted protein; lipoprotein LprH 2000675..2001298 Mycobacterium ulcerans Agy99 4551699 YP_905760.1 CDS MUL_1813 NC_008611.1 2001395 2002963 D May be regulated by LexA; Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; 13e12 repeat-containing protein 2001395..2002963 Mycobacterium ulcerans Agy99 4551700 YP_905761.1 CDS uvrC NC_008611.1 2002891 2004777 D The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision; excinuclease ABC subunit C 2002891..2004777 Mycobacterium ulcerans Agy99 4551701 YP_905762.1 CDS MUL_1815 NC_008611.1 2004774 2005694 D cytoplasmic protein; hypothetical protein 2004774..2005694 Mycobacterium ulcerans Agy99 4551702 YP_905763.1 CDS MUL_1816 NC_008611.1 2005691 2006767 D Detected in the cytoplamic fraction.; membrane protein; hypothetical protein 2005691..2006767 Mycobacterium ulcerans Agy99 4551703 YP_905764.1 CDS whiA NC_008611.1 2006764 2007741 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; involved in transcriptional mechanism; transcriptional regulatory protein WhiA 2006764..2007741 Mycobacterium ulcerans Agy99 4551704 YP_905765.1 CDS MUL_1818 NC_008611.1 2007844 2009226 D cytoplasmic protein; hypothetical protein 2007844..2009226 Mycobacterium ulcerans Agy99 4551705 YP_905766.1 CDS lipO NC_008611.1 2009227 2010504 R Detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; function unknown, but supposed involved in lipid metabolism; esterase LipO complement(2009227..2010504) Mycobacterium ulcerans Agy99 4551706 YP_905767.1 CDS MUL_1821 NC_008611.1 2012138 2012941 R cytoplasmic protein; hypothetical protein complement(2012138..2012941) Mycobacterium ulcerans Agy99 4551708 YP_905768.1 CDS MUL_1823 NC_008611.1 2014207 2014911 D cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 2014207..2014911 Mycobacterium ulcerans Agy99 4551710 YP_905769.1 CDS MUL_1824 NC_008611.1 2014936 2015253 D cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 2014936..2015253 Mycobacterium ulcerans Agy99 4551675 YP_905770.1 CDS MUL_1826 NC_008611.1 2018316 2019737 D cytoplasmic protein; function unknown, probably involved in cellular metabolism; dehydrogenase 2018316..2019737 Mycobacterium ulcerans Agy99 4551677 YP_905771.1 CDS MUL_1827 NC_008611.1 2019745 2020362 R membrane protein; hypothetical protein complement(2019745..2020362) Mycobacterium ulcerans Agy99 4551678 YP_905772.1 CDS gap NC_008611.1 2020646 2021665 D Also detected in the extracellular matrix and the membrane fraction by proteomics.; cytoplasmic protein; involved in second phase of glycolysis (first step) [catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH]; glyceraldehyde 3-phosphate dehydrogenase Gap 2020646..2021665 Mycobacterium ulcerans Agy99 4551679 YP_905773.1 CDS pgk NC_008611.1 2021678 2022928 D Converts 3-phospho-D-glycerate to 3-phospho-D-glyceroyl phosphate during the glycolysis pathway; phosphoglycerate kinase 2021678..2022928 Mycobacterium ulcerans Agy99 4551680 YP_905774.1 CDS tpiA NC_008611.1 2022925 2023710 D Reversibly isomerizes the ketone sugar dihydroxyacetone phosphate to the aldehyde sugar glyceraldehyde-3-phosphate; triosephosphate isomerase 2022925..2023710 Mycobacterium ulcerans Agy99 4551681 YP_905775.1 CDS MUL_1833 NC_008611.1 2028316 2029044 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; possibly involved in transcriptional mechanism.; GntR family transcriptional regulator 2028316..2029044 Mycobacterium ulcerans Agy99 4551684 YP_905776.1 CDS MUL_1834 NC_008611.1 2029078 2029422 R membrane protein; hypothetical protein complement(2029078..2029422) Mycobacterium ulcerans Agy99 4551685 YP_905777.1 CDS secG NC_008611.1 2029580 2029813 R membrane protein; involved in protein export. participates in a early event of protein translocation.; preprotein translocase subunit SecG complement(2029580..2029813) Mycobacterium ulcerans Agy99 4551686 YP_905778.1 CDS MUL_1836 NC_008611.1 2029883 2032690 D catalyzes the formation of oxaloacetate from phosphoenolpyruvate; phosphoenolpyruvate carboxylase 2029883..2032690 Mycobacterium ulcerans Agy99 4551687 YP_905779.1 CDS MUL_1837 NC_008611.1 2032714 2033865 R secreted protein; PE-PGRS family protein complement(2032714..2033865) Mycobacterium ulcerans Agy99 4551688 YP_905780.1 CDS MUL_1838 NC_008611.1 2033866 2034981 R secreted protein; PE-PGRS family protein complement(2033866..2034981) Mycobacterium ulcerans Agy99 4551689 YP_905781.1 CDS MUL_1839 NC_008611.1 2035077 2035475 R cytoplasmic protein; hypothetical protein complement(2035077..2035475) Mycobacterium ulcerans Agy99 4551690 YP_905782.1 CDS devB NC_008611.1 2035525 2036268 R Detected in the cytoplasmic fraction by proteomics.; cytoplasmic protein; involved in pentose phosphate pathway. hydrolysis of 6-phosphogluconolactone to 6- phosphogluconate. [catalytic activity : 6-phospho-D-glucono-1,5-lactone + H(2)O = 6- phospho-D-gluconate]; 6-phosphogluconolactonase complement(2035525..2036268) Mycobacterium ulcerans Agy99 4551691 YP_905783.1 CDS opcA NC_008611.1 2036265 2037176 R cytoplasmic protein; may be involved in the functional assembly of glucose 6-phosphate dehydrogenase; OXPP cycle protein OpcA complement(2036265..2037176) Mycobacterium ulcerans Agy99 4551692 YP_905784.1 CDS zwf2 NC_008611.1 2037226 2038779 R catalyzes the formation of D-glucono-1,5-lactone 6-phosphate from D-glucose 6-phosphate; glucose-6-phosphate 1-dehydrogenase complement(2037226..2038779) Mycobacterium ulcerans Agy99 4551662 YP_905785.1 CDS tal NC_008611.1 2038776 2039897 R catalyzes the reversible formation of D-erythrose 4-phosphate and D-fructose 6-phosphate from sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate; transaldolase complement(2038776..2039897) Mycobacterium ulcerans Agy99 4551663 YP_905786.1 CDS tkt NC_008611.1 2039913 2042015 R catalyzes the formation of ribose 5-phosphate and xylulose 5-phosphate from sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate; can transfer ketol groups between several groups; in Escherichia coli there are two tkt genes, tktA expressed during exponential growth and the tktB during stationary phase; transketolase complement(2039913..2042015) Mycobacterium ulcerans Agy99 4551664 YP_905787.1 CDS ctaB NC_008611.1 2042303 2043277 D converts protoheme IX and farnesyl diphosphate to heme O; protoheme IX farnesyltransferase 2042303..2043277 Mycobacterium ulcerans Agy99 4551665 YP_905788.1 CDS MUL_1848 NC_008611.1 2044863 2045909 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 2044863..2045909 Mycobacterium ulcerans Agy99 4551668 YP_905789.1 CDS MUL_1849 NC_008611.1 2046040 2047374 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 2046040..2047374 Mycobacterium ulcerans Agy99 4551669 YP_905790.1 CDS MUL_1853 NC_008611.1 2051104 2052150 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(2051104..2052150) Mycobacterium ulcerans Agy99 4551672 YP_905791.1 CDS MUL_1854 NC_008611.1 2054860 2055870 D cytoplasmic protein; involved in transcriptional mechanism; transcriptional activator protein 2054860..2055870 Mycobacterium ulcerans Agy99 4551673 YP_905792.1 CDS qor NC_008611.1 2056197 2057207 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; catalyzes the one electron reduction of certain quinones [catalytic activity: NADPH + quinone = NADP+ + semiquinone]; quinone reductase Qor complement(2056197..2057207) Mycobacterium ulcerans Agy99 4551674 YP_905793.1 CDS MUL_1856 NC_008611.1 2057206 2058060 D cytoplasmic protein; hypothetical protein 2057206..2058060 Mycobacterium ulcerans Agy99 4551563 YP_905794.1 CDS MUL_1857 NC_008611.1 2058010 2058939 R membrane protein; thought to be involved in active transport of antibiotic across the membrane (export): unidentified antibiotic resistance by an export mechanism. responsible for the translocation of the substrate across the membrane.; antibiotic ABC transporter transmembrane protein complement(2058010..2058939) Mycobacterium ulcerans Agy99 4551564 YP_905795.1 CDS MUL_1858 NC_008611.1 2059010 2059798 R membrane protein; thought to be involved in active transport of antibiotic across the membrane (export): unidentified antibiotic resistance by an export mechanism. responsible for the translocation of the substrate across the membrane.; antibiotic ABC transporter transmembrane protein complement(2059010..2059798) Mycobacterium ulcerans Agy99 4551565 YP_905796.1 CDS MUL_1859 NC_008611.1 2059795 2060706 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; thought to be involved in active transport of antibiotic across the membrane (export): unidentified antibiotic resistance by an export mechanism. responsible for energy coupling to the transport system.; antibiotic ABC transporter ATP-binding protein complement(2059795..2060706) Mycobacterium ulcerans Agy99 4551566 YP_905797.1 CDS MUL_1860 NC_008611.1 2060751 2062568 R membrane protein; integral membrane protein complement(2060751..2062568) Mycobacterium ulcerans Agy99 4551567 YP_905798.1 CDS MUL_1861 NC_008611.1 2062649 2063434 D cytoplasmic protein; involved in transcriptional mechanism; transcriptional regulatory protein 2062649..2063434 Mycobacterium ulcerans Agy99 4551568 YP_905799.1 CDS MUL_1862 NC_008611.1 2063431 2064876 D cytoplasmic protein; hypothetical protein 2063431..2064876 Mycobacterium ulcerans Agy99 4551569 YP_905800.1 CDS MUL_1863 NC_008611.1 2064873 2066072 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein 2064873..2066072 Mycobacterium ulcerans Agy99 4551570 YP_905801.1 CDS MUL_1864 NC_008611.1 2066069 2066851 D Detected in the extracellular matrix, the membrane and the cytoplasmic fractions by proteomics.; membrane protein; thought to be involved in active transport across the membrane. responsible for energy coupling to the transport system.; ABC transporter ATP-binding protein 2066069..2066851 Mycobacterium ulcerans Agy99 4551571 YP_905802.1 CDS csd NC_008611.1 2066854 2068107 D cytoplasmic protein; catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, l- selenocysteine, and L-selenocystine to produce L-alanine; cysteine desulfurase 2066854..2068107 Mycobacterium ulcerans Agy99 4551572 YP_905803.1 CDS MUL_1866 NC_008611.1 2068110 2068601 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; nitrogen fixation-like protein 2068110..2068601 Mycobacterium ulcerans Agy99 4551573 YP_905804.1 CDS MUL_1867 NC_008611.1 2068594 2068941 D cytoplasmic protein; hypothetical protein 2068594..2068941 Mycobacterium ulcerans Agy99 4551574 YP_905805.1 CDS fadE15 NC_008611.1 2070605 2072434 R Detected in the cytoplasmic fraction by proteomics.; cytoplasmic protein; function unknown, but involvement in lipid degradation.; acyl-CoA dehydrogenase FadE15 complement(2070605..2072434) Mycobacterium ulcerans Agy99 4551576 YP_905806.1 CDS MUL_1870 NC_008611.1 2072540 2073358 R membrane protein; PE-PGRS family protein complement(2072540..2073358) Mycobacterium ulcerans Agy99 4551577 YP_905807.1 CDS MUL_1873 NC_008611.1 2074757 2075074 R cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 complement(2074757..2075074) Mycobacterium ulcerans Agy99 4551580 YP_905808.1 CDS MUL_1874 NC_008611.1 2075099 2075803 R cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 complement(2075099..2075803) Mycobacterium ulcerans Agy99 4551581 YP_905809.1 CDS nrdH NC_008611.1 2077472 2077711 D cytoplasmic protein; involved in electron transfer system for ribonucleotide reductase system NRDEF.; glutaredoxin electron transport component of NRDEF (glutaredoxin-like protein) NrdH 2077472..2077711 Mycobacterium ulcerans Agy99 4551546 YP_905810.1 CDS nrdI NC_008611.1 2077755 2078216 D in Salmonella NrdI has a stimulatory effect on the ribonucleotide reductase activity of NrdH with NrdEF; ribonucleotide reductase stimulatory protein 2077755..2078216 Mycobacterium ulcerans Agy99 4551547 YP_905811.1 CDS nrdE NC_008611.1 2078270 2080351 D Catalyzes the rate-limiting step in dNTP synthesis; ribonucleotide-diphosphate reductase subunit alpha 2078270..2080351 Mycobacterium ulcerans Agy99 4551548 YP_905812.1 CDS MUL_1879 NC_008611.1 2080466 2081209 D cytoplasmic protein; involved in transcriptional mechanism; AsnC family transcriptional regulator 2080466..2081209 Mycobacterium ulcerans Agy99 4551549 YP_905813.1 CDS MUL_1880 NC_008611.1 2081328 2082878 D cytoplasmic protein; function unknown, involved in cellular metabolism.; monooxygenase 2081328..2082878 Mycobacterium ulcerans Agy99 4551550 YP_905814.1 CDS MUL_1881 NC_008611.1 2082868 2083407 R cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein complement(2082868..2083407) Mycobacterium ulcerans Agy99 4551551 YP_905815.1 CDS MUL_1882 NC_008611.1 2083391 2083837 R cytoplasmic protein; hypothetical protein complement(2083391..2083837) Mycobacterium ulcerans Agy99 4551552 YP_905816.1 CDS nrdF NC_008611.1 2084047 2085021 D B2 or R2 protein; type 1b enzyme; catalyzes the rate-limiting step in dNTP synthesis; converts nucleotides to deoxynucleotides; forms a homodimer and then a multimeric complex with NrdE; ribonucleotide-diphosphate reductase subunit beta 2084047..2085021 Mycobacterium ulcerans Agy99 4551553 YP_905817.1 CDS MUL_1884 NC_008611.1 2085188 2085532 D Detected in the cytoplasmic and secreted fractions by 2D-LC-MS/MS.; cytoplasmic protein; hypothetical protein 2085188..2085532 Mycobacterium ulcerans Agy99 4551554 YP_905818.1 CDS adh NC_008611.1 2085521 2086561 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; alcohol dehydrogenase catalyzes the reversible oxidation of ethanol to acetaldehyde with the concomitant reduction of NAD: ethanol + NAD = acetaldehyde + NADH. belongs to the zinc-containing 'long-chain' alcohol dehydrogenases.; NADP-dependent alcohol dehydrogenase Adh complement(2085521..2086561) Mycobacterium ulcerans Agy99 4551555 YP_905819.1 CDS fecB NC_008611.1 2086695 2087789 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; may be involved in active transport of FeIII- decitrate across the membrane (import); FEIII-dicitrate-binding periplasmic lipoprotein FecB complement(2086695..2087789) Mycobacterium ulcerans Agy99 4551556 YP_905820.1 CDS ctaD NC_008611.1 2087968 2089689 D Detected in the membrane fraction by proteomics.; cytoplasmic protein; cytochrome C oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. electrons originating in cytochrome C are transferred via the copper a center of subunit 2 and HemE a of subunit 1 to the bimetallic center formed by HemE A3 and copper B [catalytic activity: 4 ferrocytochrome C + O(2) = 2 H(2)O + 4 ferricytochrome C]; cytochrome C oxidase polypeptide I CtaD 2087968..2089689 Mycobacterium ulcerans Agy99 4551557 YP_905821.1 CDS serB2 NC_008611.1 2089748 2090977 D cytoplasmic protein; generates serine from phosphoserine [catalytic activity: phosphoserine + H(2)O = serine + phosphate]; phosphoserine phosphatase SerB2 2089748..2090977 Mycobacterium ulcerans Agy99 4551558 YP_905822.1 CDS MUL_1890 NC_008611.1 2091390 2092436 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(2091390..2092436) Mycobacterium ulcerans Agy99 4551560 YP_905823.1 CDS MUL_1891 NC_008611.1 2092829 2093308 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 2092829..2093308 Mycobacterium ulcerans Agy99 4551561 YP_905824.1 CDS MUL_1892 NC_008611.1 2093373 2095283 D membrane protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 2093373..2095283 Mycobacterium ulcerans Agy99 4551562 YP_905825.1 CDS hycD NC_008611.1 2095289 2096239 D membrane protein; involved in hydrogen metabolism; FHL pathway.; formate hydrogenlyase HycD 2095289..2096239 Mycobacterium ulcerans Agy99 4551507 YP_905826.1 CDS hycP NC_008611.1 2096244 2096906 D membrane protein; involved in hydrogen metabolism.; hydrogenase HycP 2096244..2096906 Mycobacterium ulcerans Agy99 4551508 YP_905827.1 CDS hycQ NC_008611.1 2096906 2098372 D membrane protein; possibly involved in hydrogen metabolism.; hydrogenase HycQ 2096906..2098372 Mycobacterium ulcerans Agy99 4551509 YP_905828.1 CDS MUL_1897 NC_008611.1 2099963 2100805 D membrane protein; thought to be involved in active transport of undeterminated substrate (possibly iron) across the membrane. responsible for energy coupling to the transport system.; ABC transporter ATP-binding protein 2099963..2100805 Mycobacterium ulcerans Agy99 4551511 YP_905829.1 CDS MUL_1898 NC_008611.1 2100802 2101644 D cytoplasmic protein; hypothetical protein 2100802..2101644 Mycobacterium ulcerans Agy99 4551512 YP_905830.1 CDS echA17 NC_008611.1 2101641 2102414 D Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase 2101641..2102414 Mycobacterium ulcerans Agy99 4551513 YP_905831.1 CDS MUL_1900 NC_008611.1 2102425 2103408 D cytoplasmic protein; hypothetical protein 2102425..2103408 Mycobacterium ulcerans Agy99 4551514 YP_905832.1 CDS MUL_1901 NC_008611.1 2103573 2104610 D cytoplasmic protein; hypothetical protein 2103573..2104610 Mycobacterium ulcerans Agy99 4551515 YP_905833.1 CDS MUL_1902 NC_008611.1 2104685 2105587 D C-term extended due to frame shift mutation. This CDS now overlaps d/s CDS.; Detected in the secreted protein fraction by 2D-LC- MS/MS.; secreted protein; hypothetical protein 2104685..2105587 Mycobacterium ulcerans Agy99 4551516 YP_905834.1 CDS MUL_5115 NC_008611.1 2105374 2105763 R overlaps neigbouring CDS due to frame shift mutation that extends MUL_1902.; cytoplasmic protein; hypothetical protein complement(2105374..2105763) Mycobacterium ulcerans Agy99 4551517 YP_905835.1 CDS MUL_1903 NC_008611.1 2105780 2106847 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(2105780..2106847) Mycobacterium ulcerans Agy99 4551518 YP_905836.1 CDS MUL_1904 NC_008611.1 2107273 2108010 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown, possibly involved in cellular metabolism.; transferase 2107273..2108010 Mycobacterium ulcerans Agy99 4551519 YP_905837.1 CDS MUL_1905 NC_008611.1 2108031 2108810 R membrane protein; function unknown; probably involved in lipid metabolism.; lysophospholipase complement(2108031..2108810) Mycobacterium ulcerans Agy99 4551520 YP_905838.1 CDS MUL_1906 NC_008611.1 2108835 2109380 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(2108835..2109380) Mycobacterium ulcerans Agy99 4551521 YP_905839.1 CDS phrI NC_008611.1 2109507 2110850 R cytoplasmic protein; function unknown, possible role in DNA replication, recombination, and repair.; DNA photolyase, PhrI complement(2109507..2110850) Mycobacterium ulcerans Agy99 4551522 YP_905840.1 CDS MUL_1908 NC_008611.1 2110859 2111338 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown. possibly involved in signal transduction mechanisms.; tryptophan-rich sensory protein complement(2110859..2111338) Mycobacterium ulcerans Agy99 4551523 YP_905841.1 CDS MUL_1909 NC_008611.1 2111335 2113449 R secreted protein; function unknown. thought to be involved in drug export.; transmembrane transport protein complement(2111335..2113449) Mycobacterium ulcerans Agy99 4551524 YP_905842.1 CDS MUL_1912 NC_008611.1 2115519 2116223 D cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 2115519..2116223 Mycobacterium ulcerans Agy99 4551489 YP_905843.1 CDS MUL_1913 NC_008611.1 2116248 2116565 D cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 2116248..2116565 Mycobacterium ulcerans Agy99 4551490 YP_905844.1 CDS MUL_1914 NC_008611.1 2116864 2117271 R cytoplasmic protein; hypothetical protein complement(2116864..2117271) Mycobacterium ulcerans Agy99 4551491 YP_905845.1 CDS MUL_1916 NC_008611.1 2118274 2118969 D membrane protein; hypothetical protein 2118274..2118969 Mycobacterium ulcerans Agy99 4551493 YP_905846.1 CDS MUL_1918 NC_008611.1 2120386 2121372 D Detected in the cytoplamic fraction by LCMSMS; cytoplasmic protein; function unknown, probably involved in cellular metabolism.; dehydrogenase/reductase 2120386..2121372 Mycobacterium ulcerans Agy99 4551495 YP_905847.1 CDS MUL_1919 NC_008611.1 2121369 2122613 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown, possibly involved in cellular metabolism.; transferase complement(2121369..2122613) Mycobacterium ulcerans Agy99 4551496 YP_905848.1 CDS MUL_1920 NC_008611.1 2122705 2124300 R cytoplasmic protein; hypothetical protein complement(2122705..2124300) Mycobacterium ulcerans Agy99 4551497 YP_905849.1 CDS MUL_1921 NC_008611.1 2124297 2125115 R cytoplasmic protein; hypothetical protein complement(2124297..2125115) Mycobacterium ulcerans Agy99 4551498 YP_905850.1 CDS fixA NC_008611.1 2125388 2126179 D Detected in the cytoplasmic, the membrane and the extracellular matrix fractions by proteomics.; cytoplasmic protein; the electron transfer flavoprotein serves as a specific electron acceptor for other dehydrogenases. it transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase); electron transfer flavoprotein FixA 2125388..2126179 Mycobacterium ulcerans Agy99 4551499 YP_905851.1 CDS fixB NC_008611.1 2126227 2127183 D Detected in the cytoplasmic fraction by LC-MS/MS. Also detected in the extracellular matrix and the membrane fraction by proteomics.; cytoplasmic protein; the electron transfer flavoprotein serves as a specific electron acceptor for other dehydrogenases. it transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase); electron transfer flavoprotein FixB 2126227..2127183 Mycobacterium ulcerans Agy99 4551500 YP_905852.1 CDS MUL_1924 NC_008611.1 2127284 2128144 D cytoplasmic protein; hypothetical protein 2127284..2128144 Mycobacterium ulcerans Agy99 4551501 YP_905853.1 CDS MUL_1925 NC_008611.1 2128144 2128980 D membrane protein; hypothetical protein 2128144..2128980 Mycobacterium ulcerans Agy99 4551502 YP_905854.1 CDS iscS NC_008611.1 2129621 2130802 D cytoplasmic protein; catalyzes the removal of elemental sulfur from cysteine to produce alanine.; cysteine desulfurase IscS 2129621..2130802 Mycobacterium ulcerans Agy99 4551504 YP_905855.1 CDS mnmA NC_008611.1 2130799 2131890 D catalyzes a sulfuration reaction to synthesize 2-thiouridine at the U34 position of tRNAs; tRNA-specific 2-thiouridylase MnmA 2130799..2131890 Mycobacterium ulcerans Agy99 4551505 YP_905856.1 CDS lpqA NC_008611.1 2131887 2132516 R secreted protein; lipoprotein LpqA complement(2131887..2132516) Mycobacterium ulcerans Agy99 4551506 YP_905857.1 CDS MUL_1930 NC_008611.1 2132612 2133625 D cytoplasmic protein; hypothetical protein 2132612..2133625 Mycobacterium ulcerans Agy99 4551271 YP_905858.1 CDS MUL_1932 NC_008611.1 2135365 2136411 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 2135365..2136411 Mycobacterium ulcerans Agy99 4551273 YP_905859.1 CDS ligA NC_008611.1 2137125 2139176 D this protein catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction; essential for DNA replication and repair of damaged DNA; similar to ligase LigB; NAD-dependent DNA ligase LigA 2137125..2139176 Mycobacterium ulcerans Agy99 4551274 YP_905860.1 CDS MUL_1934 NC_008611.1 2139237 2139842 D cytoplasmic protein; possibly involved in a transcriptional mechanism; regulatory protein 2139237..2139842 Mycobacterium ulcerans Agy99 4551275 YP_905861.1 CDS MUL_1935 NC_008611.1 2140039 2140443 D membrane protein; hypothetical protein 2140039..2140443 Mycobacterium ulcerans Agy99 4551276 YP_905862.1 CDS MUL_1937 NC_008611.1 2143348 2144010 R cytoplasmic protein; hypothetical protein complement(2143348..2144010) Mycobacterium ulcerans Agy99 4551278 YP_905863.1 CDS gatC NC_008611.1 2144162 2144461 D allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; some Mycoplasma proteins contain an N-terminal fusion to an unknown domain; aspartyl/glutamyl-tRNA amidotransferase subunit C 2144162..2144461 Mycobacterium ulcerans Agy99 4551279 YP_905864.1 CDS gatA NC_008611.1 2144458 2145939 D allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; aspartyl/glutamyl-tRNA amidotransferase subunit A 2144458..2145939 Mycobacterium ulcerans Agy99 4551280 YP_905865.1 CDS pfkA NC_008611.1 2146039 2147070 D catalyzes the formation of D-fructose 1,6-bisphosphate from D-fructose 6-phosphate in glycolysis; 6-phosphofructokinase 2146039..2147070 Mycobacterium ulcerans Agy99 4551281 YP_905866.1 CDS gatB NC_008611.1 2147129 2148637 D allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; aspartyl/glutamyl-tRNA amidotransferase subunit B 2147129..2148637 Mycobacterium ulcerans Agy99 4551282 YP_905867.1 CDS lppZ NC_008611.1 2149298 2150467 R membrane protein; lipoprotein LppZ complement(2149298..2150467) Mycobacterium ulcerans Agy99 4551284 YP_905868.1 CDS MUL_1944 NC_008611.1 2150650 2151498 D membrane protein; hypothetical protein 2150650..2151498 Mycobacterium ulcerans Agy99 4551285 YP_905869.1 CDS cfp6 NC_008611.1 2151507 2151866 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown function (putative membrane protein); low molecular weight protein antigen 6, Cfp6 complement(2151507..2151866) Mycobacterium ulcerans Agy99 4551286 YP_905870.1 CDS MUL_1946 NC_008611.1 2151938 2152069 D cytoplasmic protein; hypothetical protein 2151938..2152069 Mycobacterium ulcerans Agy99 4551287 YP_905871.1 CDS ilvB1 NC_008611.1 2152213 2154069 D acetolactate synthase large subunit; catalyzes the formation of 2-acetolactate from pyruvate; acetolactate synthase 1 catalytic subunit 2152213..2154069 Mycobacterium ulcerans Agy99 4551288 YP_905872.1 CDS ilvH NC_008611.1 2154069 2154578 D with IlvI catalyzes the formation of 2-acetolactate from pyruvate, the small subunit is required for full activity and valine sensitivity; E.coli produces 3 isoenzymes of acetolactate synthase which differ in specificity to substrates, valine sensitivity and affinity for cofactors; also known as acetolactate synthase 3 small subunit; acetolactate synthase 3 regulatory subunit 2154069..2154578 Mycobacterium ulcerans Agy99 4551256 YP_905873.1 CDS ilvC NC_008611.1 2154651 2155652 D catalyzes the formation of (R)-2,3-dihydroxy-3-methylbutanoate from (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and isoleucine biosynthesis; ketol-acid reductoisomerase 2154651..2155652 Mycobacterium ulcerans Agy99 4551257 YP_905874.1 CDS MUL_1950 NC_008611.1 2155739 2156341 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(2155739..2156341) Mycobacterium ulcerans Agy99 4551258 YP_905875.1 CDS serA1 NC_008611.1 2157822 2159408 D catalyzes the formation of 3-phosphonooxypyruvate from 3-phospho-D-glycerate in serine biosynthesis; can also reduce alpha ketoglutarate to form 2-hydroxyglutarate; D-3-phosphoglycerate dehydrogenase 2157822..2159408 Mycobacterium ulcerans Agy99 4551260 YP_905876.1 CDS leuB NC_008611.1 2159464 2160483 D catalyzes the oxidation of 3-isopropylmalate to 3-carboxy-4-methyl-2-oxopentanoate in leucine biosynthesis; 3-isopropylmalate dehydrogenase 2159464..2160483 Mycobacterium ulcerans Agy99 4551261 YP_905877.1 CDS MUL_1956 NC_008611.1 2161955 2162674 D cytoplasmic protein; isomerizes HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) to ohed (2-oxo-hept-3-ene-1,7-dioate); 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase 2161955..2162674 Mycobacterium ulcerans Agy99 4551263 YP_905878.1 CDS gltX NC_008611.1 2162734 2164140 D Charges one glutamine molecule and pairs it to its corresponding RNA trinucleotide during protein translation; glutamyl-tRNA synthetase 2162734..2164140 Mycobacterium ulcerans Agy99 4551264 YP_905879.1 CDS MUL_1960 NC_008611.1 2164517 2165038 R cytoplasmic protein; hypothetical protein complement(2164517..2165038) Mycobacterium ulcerans Agy99 4551267 YP_905880.1 CDS MUL_1961 NC_008611.1 2165191 2166156 D cytoplasmic protein; hypothetical protein 2165191..2166156 Mycobacterium ulcerans Agy99 4551268 YP_905881.1 CDS MUL_1963 NC_008611.1 2169323 2170369 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 2169323..2170369 Mycobacterium ulcerans Agy99 4551270 YP_905882.1 CDS MUL_1964 NC_008611.1 2170493 2171827 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(2170493..2171827) Mycobacterium ulcerans Agy99 4551184 YP_905883.1 CDS mmpS1 NC_008611.1 2171917 2172324 R membrane protein; membrane protein MmpS1 complement(2171917..2172324) Mycobacterium ulcerans Agy99 4551185 YP_905884.1 CDS bioF2_2 NC_008611.1 2172564 2173814 R cytoplasmic protein; could be involved in biotin biosynthesis (at the first step) [catalytic activity: 6-carboxyhexanoyl-CoA + L- alanine = 8-amino-7-oxononanoate + CoA + CO2]; 8-amino-7-oxononanoate synthase complement(2172564..2173814) Mycobacterium ulcerans Agy99 4551186 YP_905885.1 CDS MUL_1967 NC_008611.1 2174358 2175059 R cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein complement(2174358..2175059) Mycobacterium ulcerans Agy99 4551187 YP_905886.1 CDS leuC NC_008611.1 2175142 2176563 D dehydratase component, catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate; isopropylmalate isomerase large subunit 2175142..2176563 Mycobacterium ulcerans Agy99 4551188 YP_905887.1 CDS leuD NC_008611.1 2176588 2177184 D catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate in leucine biosynthesis; forms a heterodimer of LeuC/D; isopropylmalate isomerase small subunit 2176588..2177184 Mycobacterium ulcerans Agy99 4551189 YP_905888.1 CDS hupB NC_008611.1 2177409 2178098 D Detected in the cytoplasmic fraction by LC-MS/MS. Also detected in the extracellular matrix and the membrane fraction by proteomics.; cytoplasmic protein; this protein belongs to the histone like family of prokaryotic DNA-binding proteins which are capable of wrapping DNA to stabilize it, and prevent its denaturation under extreme environmental conditions.; DNA-binding protein HU HupB-like protein 2177409..2178098 Mycobacterium ulcerans Agy99 4551190 YP_905889.1 CDS mutT1 NC_008611.1 2178160 2179098 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown, hydrolytic enzyme. possibly involved in removal of damaged nucleotide.; hydrolase MutT1 complement(2178160..2179098) Mycobacterium ulcerans Agy99 4551191 YP_905890.1 CDS ppk NC_008611.1 2179178 2181394 R catalyzes the reversible transfer of the terminal phosphate of ATP to form a long chain polyphosphate; polyphosphate kinase complement(2179178..2181394) Mycobacterium ulcerans Agy99 4551192 YP_905891.1 CDS MUL_1973 NC_008611.1 2181473 2182126 R membrane protein; hypothetical protein complement(2181473..2182126) Mycobacterium ulcerans Agy99 4551193 YP_905892.1 CDS gpsA NC_008611.1 2182318 2183325 D catalyzes the NAD(P)H-dependent reduction of glycerol 3-phosphate to glycerone phosphate; NAD(P)H-dependent glycerol-3-phosphate dehydrogenase 2182318..2183325 Mycobacterium ulcerans Agy99 4551194 YP_905893.1 CDS ddl NC_008611.1 2183417 2184538 D D-alanine--D-alanine ligase; DdlA; DdlB; cytoplasmic; catalyzes the formation of D-alanyl-D-alanine from two D-alanines in peptidoglycan synthesis; there are two forms of this enzyme in Escherichia coli; D-alanyl-alanine synthetase A 2183417..2184538 Mycobacterium ulcerans Agy99 4551195 YP_905894.1 CDS MUL_1977 NC_008611.1 2184535 2185119 R membrane protein; hypothetical protein complement(2184535..2185119) Mycobacterium ulcerans Agy99 4551196 YP_905895.1 CDS thiL NC_008611.1 2185327 2186292 D catalyzes the formation of thiamine diphosphate from thiamine phosphate ant ATP; thiamine monophosphate kinase 2185327..2186292 Mycobacterium ulcerans Agy99 4551197 YP_905896.1 CDS MUL_1979 NC_008611.1 2186318 2186608 D cytoplasmic protein; hypothetical protein 2186318..2186608 Mycobacterium ulcerans Agy99 4551198 YP_905897.1 CDS ung NC_008611.1 2186605 2187288 D Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine; uracil-DNA glycosylase 2186605..2187288 Mycobacterium ulcerans Agy99 4551199 YP_905898.1 CDS MUL_1982 NC_008611.1 2188503 2188925 D cytoplasmic protein; function unknown; probably involved in cellular metabolism.; nitroreductase 2188503..2188925 Mycobacterium ulcerans Agy99 4551201 YP_905899.1 CDS rpmB NC_008611.1 2189081 2189275 R required for 70S ribosome assembly; 50S ribosomal protein L28 complement(2189081..2189275) Mycobacterium ulcerans Agy99 4551202 YP_905900.1 CDS recG NC_008611.1 2191253 2193484 D catalyzes branch migration in Holliday junction intermediates; ATP-dependent DNA helicase RecG 2191253..2193484 Mycobacterium ulcerans Agy99 4551166 YP_905901.1 CDS MUL_1987 NC_008611.1 2194207 2195055 R Detected in the cytoplamic fraction by LC-MS/MS.; cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase complement(2194207..2195055) Mycobacterium ulcerans Agy99 4551168 YP_905902.1 CDS MUL_1988 NC_008611.1 2195242 2195577 R cytoplasmic protein; hypothetical protein complement(2195242..2195577) Mycobacterium ulcerans Agy99 4551169 YP_905903.1 CDS lipN NC_008611.1 2195623 2196741 D cytoplasmic protein; function unknown, lipolytic enzyme involved in cellular metabolism.; lipase/esterase LipN 2195623..2196741 Mycobacterium ulcerans Agy99 4551170 YP_905904.1 CDS MUL_1990 NC_008611.1 2196928 2197695 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 2196928..2197695 Mycobacterium ulcerans Agy99 4551171 YP_905905.1 CDS MUL_1991 NC_008611.1 2197701 2198339 D membrane protein; integral membrane protein 2197701..2198339 Mycobacterium ulcerans Agy99 4551172 YP_905906.1 CDS pca NC_008611.1 2198382 2201777 D biotin-containing enzyme that catalyzes a two step carboxylation of pyruvate to oxaloacetate; pyruvate carboxylase 2198382..2201777 Mycobacterium ulcerans Agy99 4551173 YP_905907.1 CDS coaD NC_008611.1 2202402 2202875 D Catalyzes the conversion of ATP and pantetheine 4'-phosphate to diphosphate and 3'-dephospho-coA; phosphopantetheine adenylyltransferase 2202402..2202875 Mycobacterium ulcerans Agy99 4551175 YP_905908.1 CDS MUL_1995 NC_008611.1 2202949 2203131 R secreted protein; hypothetical protein complement(2202949..2203131) Mycobacterium ulcerans Agy99 4551176 YP_905909.1 CDS MUL_1997 NC_008611.1 2204219 2205400 R membrane protein; hypothetical protein complement(2204219..2205400) Mycobacterium ulcerans Agy99 4551178 YP_905910.1 CDS MUL_1999 NC_008611.1 2207326 2208123 D cytoplasmic protein; hypothetical protein 2207326..2208123 Mycobacterium ulcerans Agy99 4551180 YP_905911.1 CDS MUL_2000 NC_008611.1 2208174 2209430 R membrane protein; hypothetical protein complement(2208174..2209430) Mycobacterium ulcerans Agy99 4551181 YP_905912.1 CDS fadD29 NC_008611.1 2211634 2213496 D activates fatty acids by binding to coenzyme A; acyl-CoA synthetase 2211634..2213496 Mycobacterium ulcerans Agy99 4551183 YP_905913.1 CDS MUL_2003 NC_008611.1 2213501 2214139 D cytoplasmic protein; responsible for the direct conversion of chorismate to p-hydroxybenzoate, the substrate used in the production of glycosylated p-hydroxybenzoic acid methyl esters and structurally related phenolphthiocerol glycolipids. in M. tuberculosis, this is the sole enzymatic source of p- hydroxybenzoic acid.; chorismate pyruvate-lyase 2213501..2214139 Mycobacterium ulcerans Agy99 4551127 YP_905914.1 CDS fadD22 NC_008611.1 2214258 2216366 D activates fatty acids by binding to coenzyme A; acyl-CoA synthetase 2214258..2216366 Mycobacterium ulcerans Agy99 4551128 YP_905915.1 CDS pks15/1 NC_008611.1 2216383 2222697 D Also detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; polyketide synthase required for the elongation of p-HBAD derivatives to form p-hydroxybenzoate derivatives which are in turn converted to phenolphthiocerols by the ppsA-E locus. this is a key step in the production of phenolic glycolipids.; polyketide synthase 2216383..2222697 Mycobacterium ulcerans Agy99 4551129 YP_905916.1 CDS lppX NC_008611.1 2223112 2223801 D Also detected in the membrane fraction by proteomics.; secreted protein; lipoprotein LppX 2223112..2223801 Mycobacterium ulcerans Agy99 4551130 YP_905917.1 CDS mmpL7 NC_008611.1 2223841 2226591 R secreted protein; involved in translocation of phthiocerol dimycocerosate (DIM) in the cell wall.; transmembrane transport protein MmpL7 complement(2223841..2226591) Mycobacterium ulcerans Agy99 4551131 YP_905918.1 CDS fadD28 NC_008611.1 2226593 2228335 R activates fatty acids by binding to coenzyme A; acyl-CoA synthetase complement(2226593..2228335) Mycobacterium ulcerans Agy99 4551132 YP_905919.1 CDS MUL_2009 NC_008611.1 2228774 2229550 D cytoplasmic protein; function unknown but orthologue in M. tuberculosis H37Rv has been shown to catalyze the transfer of a methyl group onto the lipid moiety of phthiotriol dimycocerosates to form DIM.; methyltransferase 2228774..2229550 Mycobacterium ulcerans Agy99 4551133 YP_905920.1 CDS mas NC_008611.1 2230171 2236470 D At least three isoforms were detected in the cytoplasmic extract. Also detected in the membrane fraction by proteomics.; membrane protein; catalyzes the elongation of N-fatty acyl-CoA with methylamalonyl-CoA (not malonyl-CoA) as the elongating agent to form mycocerosyl lipids.; multifunctional mycocerosic acid synthase membrane-associated Mas 2230171..2236470 Mycobacterium ulcerans Agy99 4551134 YP_905921.1 CDS papA5 NC_008611.1 2236674 2237918 R required for PDIM synthesis; phthiocerol and phthiodiolone dimycocerosate esters are scaffolds used for virulence-enhancing lipids; proposed to catalyze diesterification of phthiocerol and phthiodolone with mycocerosate; functions in polyketide synthesis; acyltransferase PapA5 complement(2236674..2237918) Mycobacterium ulcerans Agy99 4551135 YP_905922.1 CDS drrC NC_008611.1 2237952 2238782 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; probably involved in active transport of phthiocerol dimycocerosate (DIM) across the membrane (export) DrrA, DrrB and DrrC may act jointly to confer daunorubicin and doxorubicin resistance by an export mechanism. probably responsible for the translocation of the substrate across the membrane and localization of dim into the cell wall.; daunorubicin-DIM-transport integral membrane protein ABC transporter DrrC complement(2237952..2238782) Mycobacterium ulcerans Agy99 4551136 YP_905923.1 CDS drrB NC_008611.1 2238779 2239648 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; probably involved in active transport of phthiocerol dimycocerosate (DIM) across the membrane (export) DrrA, DrrB and DrrC may act jointly to confer daunorubicin and doxorubicin resistance by an export mechanism. probably responsible for the translocation of the substrate across the membrane and localization of DIM into the cell wall.; daunorubicin-DIM-transport integral membrane protein ABC transporter DrrB complement(2238779..2239648) Mycobacterium ulcerans Agy99 4551137 YP_905924.1 CDS drrA NC_008611.1 2239645 2240640 R Detected in the membrane fraction by proteomics; membrane protein; probably involved in active transport of antibiotic and phthiocerol dimycocerosate (DIM) across the membrane (export) DrrA, DrrB and DrrC may act jointly to confer daunorubicin and doxorubicin resistance by an export mechanism. responsible for energy coupling to the transport system.; daunorubicin-DIM-transport ATP-binding protein ABC transporter DrrA complement(2239645..2240640) Mycobacterium ulcerans Agy99 4551138 YP_905925.1 CDS ppsE NC_008611.1 2240651 2245096 R Three isoforms of the protein were detected in the cytoplasm by proteomics. Also detected in the membrane fraction by proteomics.; cytoplasmic protein; involved in phenolpthiocerol and phthiocerol dimycocerosate (DIM) biosynthesis: extension with Malony CoA (partial reduction, decarboxylation); phenolpthiocerol synthesis type-I polyketide synthase PpsE complement(2240651..2245096) Mycobacterium ulcerans Agy99 4551139 YP_905926.1 CDS ppsD NC_008611.1 2245102 2250522 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in phenolpthiocerol and phthiocerol dimycocerosate (DIM) biosynthesis: extension with methylmalony CoA (partial reduction); phenolpthiocerol synthesis type-I polyketide synthase PpsD complement(2245102..2250522) Mycobacterium ulcerans Agy99 4551140 YP_905927.1 CDS ppsC NC_008611.1 2250522 2257127 R Detected in the cytoplasmic fraction by proteomics.; cytoplasmic protein; involved in phenolpthiocerol and phthiocerol dimycocerosate (DIM) biosynthesis: extension with Malony CoA (complete reduction); phenolpthiocerol synthesis type-I polyketide synthase PpsC complement(2250522..2257127) Mycobacterium ulcerans Agy99 4551141 YP_905928.1 CDS ppsB NC_008611.1 2257127 2261671 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; involved in phenolpthiocerol and phthiocerol dimycocerosate (DIM) biosynthesis: extension with Malony CoA (partial reduction); phenolpthiocerol synthesis type-I polyketide synthase PpsB complement(2257127..2261671) Mycobacterium ulcerans Agy99 4551142 YP_905929.1 CDS ppsA NC_008611.1 2261701 2266509 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (LC-MS/MS); cytoplasmic protein; involved in phenolpthiocerol and phthiocerol dimycocerosate (DIM) biosynthesis: extension of C18 with Malony CoA (partial reduction); phenolpthiocerol synthesis type-I polyketide synthase PpsA complement(2261701..2266509) Mycobacterium ulcerans Agy99 4551143 YP_905930.1 CDS fadD26 NC_008611.1 2266506 2268260 R activates fatty acids by binding to coenzyme A; acyl-CoA synthetase complement(2266506..2268260) Mycobacterium ulcerans Agy99 4551144 YP_905931.1 CDS MUL_2023 NC_008611.1 2268911 2269228 R cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 complement(2268911..2269228) Mycobacterium ulcerans Agy99 4551074 YP_905932.1 CDS MUL_2024 NC_008611.1 2269253 2269957 R cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 complement(2269253..2269957) Mycobacterium ulcerans Agy99 4551075 YP_905933.1 CDS tesA NC_008611.1 2270352 2271101 R cytoplasmic protein; function unknown however in M. tuberculosis H37Rv TesA interacts with the PpsE protein. PpsE is part of the phenolphthiocerol synthase PKS cluster.; thioesterase TesA complement(2270352..2271101) Mycobacterium ulcerans Agy99 4551076 YP_905934.1 CDS MUL_2026 NC_008611.1 2271191 2271508 R cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 complement(2271191..2271508) Mycobacterium ulcerans Agy99 4551077 YP_905935.1 CDS MUL_2027 NC_008611.1 2271533 2272237 R cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 complement(2271533..2272237) Mycobacterium ulcerans Agy99 4551078 YP_905936.1 CDS MUL_2028 NC_008611.1 2272726 2273469 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown, possibly involved in cell division and chromosome partitioning.; hypothetical protein 2272726..2273469 Mycobacterium ulcerans Agy99 4551079 YP_905937.1 CDS MUL_2029 NC_008611.1 2273544 2274137 D cytoplasmic protein; hypothetical protein 2273544..2274137 Mycobacterium ulcerans Agy99 4551080 YP_905938.1 CDS rnc NC_008611.1 2274134 2274865 D cytoplasmic enzyme involved in processing rRNA and some mRNAs; substrates typically have dsRNA regions; forms a homodimer; have N-terminal nuclease and C-terminal RNA-binding domains; requires magnesium as preferred ion for activity; ribonuclease III 2274134..2274865 Mycobacterium ulcerans Agy99 4551081 YP_905939.1 CDS fpg NC_008611.1 2274866 2275744 D Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases; formamidopyrimidine-DNA glycosylase 2274866..2275744 Mycobacterium ulcerans Agy99 4551082 YP_905940.1 CDS MUL_2032 NC_008611.1 2275836 2276528 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown. contains N-term OsmC-like protein domain and C-term AcyP, acylphosphatases domain.; hypothetical protein 2275836..2276528 Mycobacterium ulcerans Agy99 4551083 YP_905941.1 CDS smc NC_008611.1 2276627 2280229 D membrane protein; plays an important role in chromosome structure and partitioning. essential for chromosome partition.; chromosome partition protein Smc 2276627..2280229 Mycobacterium ulcerans Agy99 4551084 YP_905942.1 CDS ftsY NC_008611.1 2280289 2281656 D membrane protein; probably involved in the reception and insertion of a subset of proteins at the membrane: possibly membrane receptor for Ffh.; cell division protein FtsY 2280289..2281656 Mycobacterium ulcerans Agy99 4551085 YP_905943.1 CDS glnB NC_008611.1 2283229 2283567 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; in nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP by GlnD. P-II-UMP allows the deadenylylation of glutamine synthetase (gs), thus activating the enzyme. converserly, in nitrogen excess P-II is deuridylated and promotes the adenylation of gs. P-II indirectly controls the transcription of the gs gene. P-II prevents NR-II catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of GlnA. when P-II is uridylylated to P-II-UMP, these events are reversed.; nitrogen regulatory protein P-II GlnB 2283229..2283567 Mycobacterium ulcerans Agy99 4551087 YP_905944.1 CDS MUL_2038 NC_008611.1 2286125 2287861 R cytoplasmic protein; hypothetical protein complement(2286125..2287861) Mycobacterium ulcerans Agy99 4551089 YP_905945.1 CDS MUL_2039 NC_008611.1 2288077 2288292 R cytoplasmic protein; hypothetical protein complement(2288077..2288292) Mycobacterium ulcerans Agy99 4551090 YP_905946.1 CDS ffh NC_008611.1 2288431 2290002 D Also detected in the cytoplasmic fraction by 2D-LC- MS/MS; cytoplasmic protein; necessary for efficient export of extra-cytoplasmic proteins. binds to the signal sequence when it emerges from the ribosomes.; signal recognition particle protein Ffh 2288431..2290002 Mycobacterium ulcerans Agy99 4551091 YP_905947.1 CDS MUL_2041 NC_008611.1 2290009 2291100 D cytoplasmic protein; hypothetical protein 2290009..2291100 Mycobacterium ulcerans Agy99 4551055 YP_905948.1 CDS pknI NC_008611.1 2291149 2292954 D membrane protein; involved in signal transduction (via phosphorylation) thought to be involved in cell division/differentiation [catalytic activity: ATP + a protein = ADP + a phosphoprotein]; Ser/Thr protein kinase 2291149..2292954 Mycobacterium ulcerans Agy99 4551056 YP_905949.1 CDS MUL_2043 NC_008611.1 2293060 2294853 D cytoplasmic protein; hydrolizes a specific D-amino acid.; D-amino acid aminohydrolase 2293060..2294853 Mycobacterium ulcerans Agy99 4551057 YP_905950.1 CDS MUL_2044 NC_008611.1 2294855 2295457 D cytoplasmic protein; thought to be involved in transcriptional mechanism.; TetR family transcriptional regulator 2294855..2295457 Mycobacterium ulcerans Agy99 4551058 YP_905951.1 CDS dacB NC_008611.1 2295477 2296349 R secreted protein; involved in peptidoglycan synthesis (at final stages) hydrolyzes the bound D-alanyl-D-alanine [catalytic activity: D-alanyl-D-alanine + H(2)O = 2 D- alanine]; D-alanyl-D-alanine carboxypeptidase complement(2295477..2296349) Mycobacterium ulcerans Agy99 4551059 YP_905952.1 CDS MUL_2046 NC_008611.1 2296406 2296840 D cytoplasmic protein; hypothetical protein 2296406..2296840 Mycobacterium ulcerans Agy99 4551060 YP_905953.1 CDS rpsP NC_008611.1 2297046 2297531 D binds to lower part of 30S body where it stabilizes two domains; required for efficient assembly of 30S; in Escherichia coli this protein has nuclease activity; 30S ribosomal protein S16 2297046..2297531 Mycobacterium ulcerans Agy99 4551061 YP_905954.1 CDS MUL_2048 NC_008611.1 2297540 2297782 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein 2297540..2297782 Mycobacterium ulcerans Agy99 4551062 YP_905955.1 CDS rimM NC_008611.1 2297807 2298331 D Essential for efficient processing of 16S rRNA; 16S rRNA-processing protein RimM 2297807..2298331 Mycobacterium ulcerans Agy99 4551063 YP_905956.1 CDS trmD NC_008611.1 2298340 2299032 D methylates guanosine-37 in various tRNAs; uses S-adenosyl-L-methionine to transfer methyl group to tRNA; tRNA (guanine-N(1)-)-methyltransferase 2298340..2299032 Mycobacterium ulcerans Agy99 4551064 YP_905957.1 CDS lppW NC_008611.1 2299144 2300103 R secreted protein; alanine rich lipoprotein LppW complement(2299144..2300103) Mycobacterium ulcerans Agy99 4551065 YP_905958.1 CDS rplS NC_008611.1 2300461 2300802 D this protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site; 50S ribosomal protein L19 2300461..2300802 Mycobacterium ulcerans Agy99 4551066 YP_905959.1 CDS lepB NC_008611.1 2300914 2301777 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; cleavage of N-terminal leader sequences from secreted protein precursors.; signal peptidase I LepB 2300914..2301777 Mycobacterium ulcerans Agy99 4551067 YP_905960.1 CDS rnhB NC_008611.1 2301780 2302499 D RNH2; RNase HII; binds manganese; endonuclease which specifically degrades the RNA of RNA-DNA hybrids; ribonuclease HII 2301780..2302499 Mycobacterium ulcerans Agy99 4551068 YP_905961.1 CDS MUL_2055 NC_008611.1 2302619 2302924 D cytoplasmic protein; hypothetical protein 2302619..2302924 Mycobacterium ulcerans Agy99 4551069 YP_905962.1 CDS MUL_2057 NC_008611.1 2303798 2305156 R cytoplasmic protein; hypothetical protein complement(2303798..2305156) Mycobacterium ulcerans Agy99 4551071 YP_905963.1 CDS MUL_2058 NC_008611.1 2305274 2306254 D membrane protein; function unknown, possibly involved in galactose metabolism.; hypothetical protein 2305274..2306254 Mycobacterium ulcerans Agy99 4551072 YP_905964.1 CDS fdhD NC_008611.1 2306389 2307210 D involved in the production or activity of formate dehydrogenase-H which is active when nitrate is not present during anaerobic growth; formate dehydrogenase accessory protein 2306389..2307210 Mycobacterium ulcerans Agy99 4551073 YP_905965.1 CDS MUL_2060 NC_008611.1 2307327 2307695 D cytoplasmic protein; hypothetical protein 2307327..2307695 Mycobacterium ulcerans Agy99 4551019 YP_905966.1 CDS MUL_2062 NC_008611.1 2309203 2310378 D membrane protein; hypothetical protein 2309203..2310378 Mycobacterium ulcerans Agy99 4551021 YP_905967.1 CDS viuB NC_008611.1 2310411 2311259 D Also detected in the extracellular matrix by proteomics.; cytoplasmic protein; thought to be involved in intracellular removal of iron from iron-mycobactin complex. mycobactin is an iron- chelating compound involved in the transport of iron from the bacterial environment into the cell cytoplasm.; mycobactin utilization protein ViuB 2310411..2311259 Mycobacterium ulcerans Agy99 4551022 YP_905968.1 CDS xerC NC_008611.1 2312473 2313381 D site-specific tyrosine recombinase which cuts and rejoins DNA molecules; binds cooperatively to specific DNA consensus sites; forms a heterotetrameric complex with XerC; XerCD exhibit similar sequences; essential to convert chromosome dimers to monomers during cell division and functions during plasmid segregation; cell division protein FtsK may regulate the XerCD complex; enzyme from Streptococcus group has unusual active site motifs; site-specific tyrosine recombinase XerC 2312473..2313381 Mycobacterium ulcerans Agy99 4551024 YP_905969.1 CDS rpsB NC_008611.1 2314218 2315033 D one of the last subunits in the assembly of the 30S subunit; absence of S2 does not inhibit assembly but results in an inactive subunit; 30S ribosomal protein S2 2314218..2315033 Mycobacterium ulcerans Agy99 4551026 YP_905970.1 CDS tsf NC_008611.1 2315035 2315850 D EF-Ts; functions during elongation stage of protein translation; forms a dimer; associates with EF-Tu-GDP complex and promotes exchange of GDP to GTP resulting in regeneration of the active form of EF-Tu; elongation factor Ts 2315035..2315850 Mycobacterium ulcerans Agy99 4551027 YP_905971.1 CDS amiC NC_008611.1 2315871 2317289 D catalyzes the hydrolysis of a monocarboxylic acid amid to form a monocarboxylate and ammonia; amidase 2315871..2317289 Mycobacterium ulcerans Agy99 4551028 YP_905972.1 CDS MUL_2070 NC_008611.1 2317305 2317733 R membrane protein; involved in transcriptional mechanism.; transcriptional regulatory protein complement(2317305..2317733) Mycobacterium ulcerans Agy99 4551029 YP_905973.1 CDS MUL_2071 NC_008611.1 2318013 2318417 D membrane protein; hypothetical protein 2318013..2318417 Mycobacterium ulcerans Agy99 4551030 YP_905974.1 CDS MUL_2072 NC_008611.1 2318705 2319565 D membrane protein; hypothetical protein 2318705..2319565 Mycobacterium ulcerans Agy99 4551031 YP_905975.1 CDS MUL_2073 NC_008611.1 2319566 2320306 R cytoplasmic protein; involved in transcriptional mechanism; transcriptional regulatory protein complement(2319566..2320306) Mycobacterium ulcerans Agy99 4551032 YP_905976.1 CDS pyrH NC_008611.1 2320486 2321556 D Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); cytoplasmic protein; uridine monophosphate kinase [catalytic activity: ATP + ump = ADP + UDP]; uridylate kinase PyrH 2320486..2321556 Mycobacterium ulcerans Agy99 4551033 YP_905977.1 CDS frr NC_008611.1 2321621 2322178 D Rrf; Frr; ribosome-recycling factor; release factor 4; RF4; recycles ribosomes upon translation termination along with release factor RF-3 and elongation factor EF-G; A GTPase-dependent process results in release of 50S from 70S; inhibited by release factor RF-1; essential for viability; structurally similar to tRNAs; ribosome recycling factor 2321621..2322178 Mycobacterium ulcerans Agy99 4551034 YP_905978.1 CDS cdsA NC_008611.1 2322211 2323125 D membrane protein; involved in the phospholipid biosynthesis [catalytic activity: CTP + phosphatidate = pyrophosphate + CDP-diacylglycerol]; integral membrane phosphatidate cytidylyltransferase CdsA 2322211..2323125 Mycobacterium ulcerans Agy99 4551035 YP_905979.1 CDS MUL_2078 NC_008611.1 2324231 2325325 D 23S rRNA m2A2503 methyltransferase; methylates the C2 position of the A2530 nucleotide in 23S rRNA; may be involved in antibiotic resistance; ribosomal RNA large subunit methyltransferase N 2324231..2325325 Mycobacterium ulcerans Agy99 4551001 YP_905980.1 CDS mpt53 NC_008611.1 2325505 2325978 D secreted protein; not really known. despite a weak homology to thioredoxin this cannot serve as a substrate for thioredoxin reductase. furthermore it has no disulfide reducing activity.; soluble secreted antigen Mpt53 precursor 2325505..2325978 Mycobacterium ulcerans Agy99 4551002 YP_905981.1 CDS MUL_2080 NC_008611.1 2325992 2326864 D membrane protein; function unknown, possibly involved in transport of mercury across the membrane.; integral membrane protein 2325992..2326864 Mycobacterium ulcerans Agy99 4551003 YP_905982.1 CDS MUL_2081 NC_008611.1 2326880 2327302 D cytoplasmic protein; hypothetical protein 2326880..2327302 Mycobacterium ulcerans Agy99 4551004 YP_905983.1 CDS MUL_2083 NC_008611.1 2327800 2328846 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 2327800..2328846 Mycobacterium ulcerans Agy99 4551006 YP_905984.1 CDS dipZ NC_008611.1 2329370 2331121 R membrane protein; may be involved in cytochrome-C biogenesis.; integral membrane C-type cytochrome biogenesis protein DipZ complement(2329370..2331121) Mycobacterium ulcerans Agy99 4551007 YP_905985.1 CDS dxr NC_008611.1 2331209 2332417 D catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate; 1-deoxy-D-xylulose 5-phosphate reductoisomerase 2331209..2332417 Mycobacterium ulcerans Agy99 4551008 YP_905986.1 CDS MUL_2086 NC_008611.1 2332437 2333651 D membrane protein; transmembrane protein 2332437..2333651 Mycobacterium ulcerans Agy99 4551009 YP_905987.1 CDS ispG NC_008611.1 2333746 2334837 D catalyzes the conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate into 4-hydroxy-3-methyl-2-en-1-yl diphosphate; involved in isoprenoid synthesis; 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase 2333746..2334837 Mycobacterium ulcerans Agy99 4551010 YP_905988.1 CDS MUL_2088 NC_008611.1 2334889 2335743 D cytoplasmic protein; hypothetical protein 2334889..2335743 Mycobacterium ulcerans Agy99 4551011 YP_905989.1 CDS MUL_2089 NC_008611.1 2335854 2337668 D secreted protein; function unknown, possibly involved in cell wall biosynthesis.; penicillin-binding lipoprotein 2335854..2337668 Mycobacterium ulcerans Agy99 4551012 YP_905990.1 CDS MUL_2090 NC_008611.1 2337780 2338364 D cytoplasmic protein; hypothetical protein 2337780..2338364 Mycobacterium ulcerans Agy99 4551013 YP_905991.1 CDS mapB NC_008611.1 2338511 2339326 D cytoplasmic protein; removes the amino-terminal methionine from nascent proteins [catalytic activity: L-methionylpeptide + H(2)O = L-methionine + peptide]; methionine aminopeptidase MapB 2338511..2339326 Mycobacterium ulcerans Agy99 4551014 YP_905992.1 CDS MUL_2092 NC_008611.1 2339533 2340084 D membrane protein; hypothetical protein 2339533..2340084 Mycobacterium ulcerans Agy99 4551015 YP_905993.1 CDS MUL_2093 NC_008611.1 2340217 2340954 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; amidotransferase 2340217..2340954 Mycobacterium ulcerans Agy99 4551016 YP_905994.1 CDS MUL_2094 NC_008611.1 2341023 2341991 R membrane protein; PPE family protein complement(2341023..2341991) Mycobacterium ulcerans Agy99 4551017 YP_905995.1 CDS MUL_2095 NC_008611.1 2342273 2342821 R membrane protein; hypothetical protein complement(2342273..2342821) Mycobacterium ulcerans Agy99 4551018 YP_905996.1 CDS MUL_2096 NC_008611.1 2342876 2344042 R membrane protein; PPE family protein complement(2342876..2344042) Mycobacterium ulcerans Agy99 4550982 YP_905997.1 CDS MUL_2097 NC_008611.1 2344282 2344713 R membrane protein; hypothetical protein complement(2344282..2344713) Mycobacterium ulcerans Agy99 4550983 YP_905998.1 CDS MUL_2101 NC_008611.1 2348906 2349340 D cytoplasmic protein; hypothetical protein 2348906..2349340 Mycobacterium ulcerans Agy99 4550987 YP_905999.1 CDS MUL_2106 NC_008611.1 2352209 2352322 D hypothetical protein 2352209..2352322 Mycobacterium ulcerans Agy99 4550990 YP_906000.1 CDS MUL_2108 NC_008611.1 2353829 2354851 R cytoplasmic protein; hypothetical protein complement(2353829..2354851) Mycobacterium ulcerans Agy99 4550992 YP_906001.2 CDS mqo NC_008611.1 2355048 2356709 D malate dehydrogenase; catalyzes the oxidation of malate to oxaloacetate; malate:quinone oxidoreductase 2355048..2356709 Mycobacterium ulcerans Agy99 4550993 YP_906002.1 CDS elaA NC_008611.1 2356586 2356996 D cytoplasmic protein; function unknown, predicted acyltransferase activity; acyltransferase ElaA 2356586..2356996 Mycobacterium ulcerans Agy99 4550994 YP_906003.1 CDS MUL_2111 NC_008611.1 2356993 2358843 D cytoplasmic protein; function unknown, possibly introduces a magnesium ion into specific substrate/compound.; magnesium chelatase 2356993..2358843 Mycobacterium ulcerans Agy99 4550995 YP_906004.1 CDS cobO NC_008611.1 2358865 2359479 D catalyzes the formation of adenosylcob(III)yrinic acid a,c-diamide from cob(I)yrinic acid a,c-diamide; cob(I)yrinic acid a,c-diamide adenosyltransferase 2358865..2359479 Mycobacterium ulcerans Agy99 4550996 YP_906005.1 CDS cobB NC_008611.1 2359574 2360950 D responsible for the amidation of carboxylic groups at position A and C of cobyrinic acid or hydrogenobrynic acid; cobyrinic acid a,c-diamide synthase 2359574..2360950 Mycobacterium ulcerans Agy99 4550997 YP_906006.1 CDS cysG NC_008611.1 2361128 2362333 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in the biosynthesis of siroheme and cobalamin [catalytic activity: 2 S-adenosyl-L-methionine + uroporphyrin III = 2 S-adenosyl-L-homocysteine + sirohydrochlorin] SAM-dependent methyl transferase that methylates uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. it catalyzes also the conversion of precorrin-2 into siroheme (consisting of an oxidation and fe(2+) chelation); multifunctional enzyme siroheme synthase CysG 2361128..2362333 Mycobacterium ulcerans Agy99 4550998 YP_906007.1 CDS efpA NC_008611.1 2362367 2363959 D membrane protein; thought to be involved in transport of undeterminated substrate (possibly drug) across the membrane (export): so responsible for the translocation of the substrate across the membrane.; integral membrane efflux protein EfpA 2362367..2363959 Mycobacterium ulcerans Agy99 4550999 YP_906008.1 CDS proS NC_008611.1 2364066 2365814 D catalyzes the formation of prolyl-tRNA(Pro) from proline and tRNA(Pro); prolyl-tRNA synthetase 2364066..2365814 Mycobacterium ulcerans Agy99 4551000 YP_906009.1 CDS MUL_2117 NC_008611.1 2365827 2366315 R cytoplasmic protein; hypothetical protein complement(2365827..2366315) Mycobacterium ulcerans Agy99 4550945 YP_906010.1 CDS MUL_2118 NC_008611.1 2366312 2366818 R membrane protein; hypothetical protein complement(2366312..2366818) Mycobacterium ulcerans Agy99 4550946 YP_906011.1 CDS MUL_2119 NC_008611.1 2367070 2367603 D in Streptococcus pneumoniae this gene was found to be essential; structure determination of the Streptococcus protein shows that it is similar to a number of other proteins; hypothetical protein 2367070..2367603 Mycobacterium ulcerans Agy99 4550947 YP_906012.1 CDS nusA NC_008611.1 2367600 2368643 D modifies transcription through interactions with RNA polymerase affecting elongation, readthrough, termination, and antitermination; transcription elongation factor NusA 2367600..2368643 Mycobacterium ulcerans Agy99 4550948 YP_906013.1 CDS MUL_2121 NC_008611.1 2368822 2369109 D cytoplasmic protein; hypothetical protein 2368822..2369109 Mycobacterium ulcerans Agy99 4550949 YP_906014.1 CDS infB NC_008611.1 2369174 2372011 D Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits during initiation of protein synthesis. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex; translation initiation factor IF-2 2369174..2372011 Mycobacterium ulcerans Agy99 4550950 YP_906015.1 CDS rbfA NC_008611.1 2372024 2372551 D associates with free 30S ribosomal subunits; essential for efficient processing of 16S rRNA; in Escherichia coli rbfA is induced by cold shock; ribosome-binding factor A 2372024..2372551 Mycobacterium ulcerans Agy99 4550951 YP_906016.1 CDS MUL_2124 NC_008611.1 2372571 2373530 D cytoplasmic protein; hypothetical protein 2372571..2373530 Mycobacterium ulcerans Agy99 4550952 YP_906017.1 CDS dinF NC_008611.1 2373546 2374883 D membrane protein; function unknown, induction by DNA damage.; DNA-damage-inducible protein F DinF 2373546..2374883 Mycobacterium ulcerans Agy99 4550953 YP_906018.1 CDS ugpA NC_008611.1 2374948 2375847 D membrane protein; involved in active transport of SN-glycerol-3- phosphate across the membrane (import) responsible for the translocation of the substrate across the membrane.; SN-glycerol-3-phosphate transport integral membrane protein ABC transporter UgpA 2374948..2375847 Mycobacterium ulcerans Agy99 4550954 YP_906019.1 CDS MUL_2128 NC_008611.1 2376320 2377366 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(2376320..2377366) Mycobacterium ulcerans Agy99 4550956 YP_906020.1 CDS MUL_2129 NC_008611.1 2377843 2379018 D membrane protein; PPE family protein 2377843..2379018 Mycobacterium ulcerans Agy99 4550957 YP_906021.1 CDS MUL_2133 NC_008611.1 2382612 2382932 R cytoplasmic protein; hypothetical protein complement(2382612..2382932) Mycobacterium ulcerans Agy99 4550961 YP_906022.1 CDS MUL_2134 NC_008611.1 2383259 2384971 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; hydrolase complement(2383259..2384971) Mycobacterium ulcerans Agy99 4550962 YP_906023.1 CDS MUL_2135 NC_008611.1 2385003 2385578 R secreted protein; hypothetical protein complement(2385003..2385578) Mycobacterium ulcerans Agy99 4550963 YP_906024.1 CDS MUL_2136 NC_008611.1 2385773 2386084 D cytoplasmic protein; hypothetical protein 2385773..2386084 Mycobacterium ulcerans Agy99 4550927 YP_906025.1 CDS lppV_1 NC_008611.1 2387760 2388335 D secreted protein; lipoprotein LppV 2387760..2388335 Mycobacterium ulcerans Agy99 4550929 YP_906026.1 CDS lppV NC_008611.1 2388393 2388965 D secreted protein; lipoprotein LppV 2388393..2388965 Mycobacterium ulcerans Agy99 4550930 YP_906027.1 CDS MUL_2140 NC_008611.1 2389095 2390021 D cytoplasmic protein; hypothetical protein 2389095..2390021 Mycobacterium ulcerans Agy99 4550931 YP_906028.1 CDS pptII NC_008611.1 2390018 2390701 D cytoplasmic protein; phosphopantetheinyl transferase. secondary metabolites biosynthesis, transport, and catabolism. Sfp- type phosphopantetheinyl transferase; phosphopantetheinyl transferase, PptII 2390018..2390701 Mycobacterium ulcerans Agy99 4550932 YP_906029.1 CDS truB NC_008611.1 2390698 2391603 D catalyzes isomerization of specific uridines in RNA to pseudouridine; responsible for residues in T loops of many tRNAs; tRNA pseudouridine synthase B 2390698..2391603 Mycobacterium ulcerans Agy99 4550933 YP_906030.1 CDS ltp1 NC_008611.1 2391710 2392912 D membrane protein; possibly catalyzes the transfer of a great variety of lipids between membranes.; lipid-transfer protein 2391710..2392912 Mycobacterium ulcerans Agy99 4550934 YP_906031.1 CDS fadE21 NC_008611.1 2392941 2394179 D membrane protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE21 2392941..2394179 Mycobacterium ulcerans Agy99 4550935 YP_906032.1 CDS sirR NC_008611.1 2394196 2394888 R cytoplasmic protein; involved in transcriptional mechanism.; transcriptional repressor SirR complement(2394196..2394888) Mycobacterium ulcerans Agy99 4550936 YP_906033.1 CDS ribF NC_008611.1 2395069 2396043 D catalyzes the formation of FMN from riboflavin and the formation of FAD from FMN; in Bacillus the ribC gene has both flavokinase and FAD synthetase activities; bifunctional riboflavin kinase/FMN adenylyltransferase 2395069..2396043 Mycobacterium ulcerans Agy99 4550937 YP_906034.1 CDS rpsO NC_008611.1 2396170 2396439 D primary rRNA binding protein; helps nucleate assembly of 30S; binds directly to the 16S rRNA and an intersubunit bridge to the 23S rRNA; autoregulates translation through interactions with the mRNA leader sequence; 30S ribosomal protein S15 2396170..2396439 Mycobacterium ulcerans Agy99 4550938 YP_906035.1 CDS lppU NC_008611.1 2396451 2396984 D secreted protein; lipoprotein LppU 2396451..2396984 Mycobacterium ulcerans Agy99 4550939 YP_906036.1 CDS MUL_2149 NC_008611.1 2397019 2397417 D cytoplasmic protein; hypothetical protein 2397019..2397417 Mycobacterium ulcerans Agy99 4550940 YP_906037.1 CDS gpsI NC_008611.1 2397815 2400076 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS) Also detected in the cytoplamic fraction by LC- MS/MS and in the extracellular matrix fraction by proteomics.; cytoplasmic protein; involved in mRNA degradation. hydrolyses single- stranded polyribonucleotides processively in the 3' to 5' direction. involved in the RNA degradosome, a multi-enzyme complex important in RNA processing and messenger RNA degradation [catalytic activity: RNA(n+1) + phosphate = RNA(N) + a nucleoside diphosphate]; polynucleotide phosphorylase/polyadenylase 2397815..2400076 Mycobacterium ulcerans Agy99 4550941 YP_906038.1 CDS MUL_2152 NC_008611.1 2401151 2402197 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(2401151..2402197) Mycobacterium ulcerans Agy99 4550943 YP_906039.1 CDS MUL_2153 NC_008611.1 2402751 2403785 D secreted protein; hypothetical protein 2402751..2403785 Mycobacterium ulcerans Agy99 4550944 YP_906040.1 CDS ald NC_008611.1 2403862 2404977 R Also detected in the cytoplasmic and membrane fractions by proteomics.; secreted protein; may play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer [catalytic activity: L-alanine + H(2)O + NAD(+) = pyruvate + NH(3) + NADH]; secreted L-alanine dehydrogenase Ald (40 kDa antigen) complement(2403862..2404977) Mycobacterium ulcerans Agy99 4550816 YP_906041.1 CDS MUL_2155 NC_008611.1 2405173 2405607 D cytoplasmic protein; involved in transcriptional mechanism.; Lrp/AsnC family transcriptional regulator 2405173..2405607 Mycobacterium ulcerans Agy99 4550817 YP_906042.1 CDS MUL_2156 NC_008611.1 2405636 2406100 D Also detected in the membrane fraction by proteomics.; cytoplasmic protein; hypothetical protein 2405636..2406100 Mycobacterium ulcerans Agy99 4550818 YP_906043.1 CDS MUL_2157 NC_008611.1 2406205 2407122 D cytoplasmic protein; hypothetical protein 2406205..2407122 Mycobacterium ulcerans Agy99 4550819 YP_906044.1 CDS MUL_2159 NC_008611.1 2408901 2409983 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 2408901..2409983 Mycobacterium ulcerans Agy99 4550821 YP_906045.1 CDS MUL_2160 NC_008611.1 2409989 2410630 R cytoplasmic protein; hypothetical protein complement(2409989..2410630) Mycobacterium ulcerans Agy99 4550822 YP_906046.1 CDS MUL_2161 NC_008611.1 2410751 2411776 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 2410751..2411776 Mycobacterium ulcerans Agy99 4550823 YP_906047.1 CDS MUL_2162 NC_008611.1 2411847 2412872 D cytoplasmic protein; hypothetical protein 2411847..2412872 Mycobacterium ulcerans Agy99 4550824 YP_906048.1 CDS dapB NC_008611.1 2414320 2415057 D catalyzes the reduction of 2,3-dihydrodipicolinate to 2,3,4,5-tetrahydrodipicolinate in lysine and diaminopimelate biosynthesis; dihydrodipicolinate reductase 2414320..2415057 Mycobacterium ulcerans Agy99 4550826 YP_906049.1 CDS MUL_2165 NC_008611.1 2415065 2415529 D secreted protein; hypothetical protein 2415065..2415529 Mycobacterium ulcerans Agy99 4550827 YP_906050.1 CDS MUL_2166 NC_008611.1 2415526 2415990 D cytoplasmic protein; hypothetical protein 2415526..2415990 Mycobacterium ulcerans Agy99 4550828 YP_906051.1 CDS MUL_2167 NC_008611.1 2416526 2417647 D PPE44; membrane protein; function unknown. may be involved in virulence; PPE family protein 2416526..2417647 Mycobacterium ulcerans Agy99 4550830 YP_906052.1 CDS MUL_2170 NC_008611.1 2420473 2421519 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(2420473..2421519) Mycobacterium ulcerans Agy99 4550833 YP_906053.1 CDS fabG NC_008611.1 2425638 2426420 D Catalyzes the first of the two reduction steps in the elongation cycle of fatty acid synthesis; 3-ketoacyl-ACP reductase 2425638..2426420 Mycobacterium ulcerans Agy99 4550642 YP_906054.1 CDS MUL_2175 NC_008611.1 2426816 2427862 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(2426816..2427862) Mycobacterium ulcerans Agy99 4550644 YP_906055.1 CDS fadE18_1 NC_008611.1 2428983 2430086 D cytoplasmic protein; function unknown, but supposed involvement in lipid degradation.; acyl-CoA dehydrogenase FadE18 2428983..2430086 Mycobacterium ulcerans Agy99 4550645 YP_906056.1 CDS MUL_2177 NC_008611.1 2430109 2430849 R Detected in the secreted fraction by proteomics.; secreted protein; function unknown, probably involved in cellular metabolism.; alanine rich hydrolase complement(2430109..2430849) Mycobacterium ulcerans Agy99 4550646 YP_906057.1 CDS thyA NC_008611.1 2430948 2431748 D ThyA; catalyzes formation of dTMP and 7,8-dihydrofolate from 5,10-methylenetetrahydrofolate and dUMP; involved in deoxyribonucleotide biosynthesis; there are 2 copies in some Bacilli, one of which appears to be phage-derived; thymidylate synthase 2430948..2431748 Mycobacterium ulcerans Agy99 4550647 YP_906058.1 CDS dfrA NC_008611.1 2431756 2432253 D cytoplasmic protein; essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dump to dTMP [catalytic activity: 5,6,7,8- tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH]; dihydrofolate reductase DfrA 2431756..2432253 Mycobacterium ulcerans Agy99 4550648 YP_906059.1 CDS MUL_2180 NC_008611.1 2432250 2433464 D cytoplasmic protein; hypothetical protein 2432250..2433464 Mycobacterium ulcerans Agy99 4550649 YP_906060.1 CDS thyX NC_008611.1 2436003 2436764 D flavin dependent thymidylate synthase; ThyX; thymidylate synthase complementing protein; catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate; the enzyme from Mycobacterium tuberculosis forms homotetramers; uses FAD as a cofactor; FAD-dependent thymidylate synthase 2436003..2436764 Mycobacterium ulcerans Agy99 4550652 YP_906061.1 CDS dapA NC_008611.1 2436882 2437784 D catalyzes the formation of dihydrodipicolinate from L-aspartate 4-semialdehyde and pyruvate in lysine and diaminopimelate biosynthesis; dihydrodipicolinate synthase 2436882..2437784 Mycobacterium ulcerans Agy99 4550653 YP_906062.1 CDS MUL_2185 NC_008611.1 2437850 2438167 R cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 complement(2437850..2438167) Mycobacterium ulcerans Agy99 4550654 YP_906063.1 CDS MUL_2186 NC_008611.1 2438192 2438896 R cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 complement(2438192..2438896) Mycobacterium ulcerans Agy99 4550655 YP_906064.1 CDS MUL_2188 NC_008611.1 2440037 2440486 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(2440037..2440486) Mycobacterium ulcerans Agy99 4550623 YP_906065.1 CDS furA NC_008611.1 2440852 2441268 D cytoplasmic protein; acts as a global negative controlling element, employing fe(2+) as a cofactor to bind the operator of the repressed genes. seems to regulate transcription of KatG gene.; ferric uptake regulation protein FurA 2440852..2441268 Mycobacterium ulcerans Agy99 4550624 YP_906066.1 CDS katG NC_008611.1 2441410 2443536 D Also detected in the secreted protein fraction by 2D-LC-MS/MS.; cytoplasmic protein; multifunctional enzyme, exhibiting both a catalase, a broad-spectrum peroxidase, and a peroxynitritase activities. may play a role in the intracellular survival of mycobacteria within macrophages; protection against reactive oxygen and nitrogen intermediates produced by phagocytic cells. seems regulated by SigB [catalytic activity: 2 H(2)O(2) = O(2) + 2 H(2)O]; catalase-peroxidase-peroxynitritase T KatG 2441410..2443536 Mycobacterium ulcerans Agy99 4550625 YP_906067.1 CDS betP NC_008611.1 2443443 2445380 R membrane protein; high-affinity uptake of glycine betaine. supposed responsible for the translocation of the substrate across the membrane.; glycine betaine transport integral membrane protein BetP complement(2443443..2445380) Mycobacterium ulcerans Agy99 4550626 YP_906068.1 CDS MUL_2192 NC_008611.1 2445727 2446773 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(2445727..2446773) Mycobacterium ulcerans Agy99 4550627 YP_906069.1 CDS MUL_2196 NC_008611.1 2449447 2450493 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 2449447..2450493 Mycobacterium ulcerans Agy99 4550630 YP_906070.1 CDS MUL_2197 NC_008611.1 2453245 2453793 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(2453245..2453793) Mycobacterium ulcerans Agy99 4550631 YP_906071.1 CDS MUL_2198 NC_008611.1 2453903 2454532 R cytoplasmic protein; function unknown, probably involved in cellular metabolism; nitroreductase complement(2453903..2454532) Mycobacterium ulcerans Agy99 4550632 YP_906072.1 CDS MUL_2200 NC_008611.1 2455743 2457707 D membrane protein; involved in signal transduction (via phosphorylation) thought to be involved in membrane transport. phosphorylates the peptide substrate myelin basic protein (MBP) at serine and threonine residues [catalytic activity: ATP + a protein = ADP + a phosphoprotein]; membrane-anchored serine/threonine-protein kinase 2455743..2457707 Mycobacterium ulcerans Agy99 4550634 YP_906073.1 CDS MUL_2201 NC_008611.1 2457915 2458244 R cytoplasmic protein; function unknown.; hypothetical protein complement(2457915..2458244) Mycobacterium ulcerans Agy99 4550635 YP_906074.1 CDS MUL_2202 NC_008611.1 2458333 2458905 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); cytoplasmic protein; hypothetical protein 2458333..2458905 Mycobacterium ulcerans Agy99 4550636 YP_906075.1 CDS MUL_2205 NC_008611.1 2462531 2463001 R Also detected in the extracellular matrix and membrane fraction by proteomics.; cytoplasmic protein; hypothetical protein complement(2462531..2463001) Mycobacterium ulcerans Agy99 4550639 YP_906076.1 CDS MUL_2208 NC_008611.1 2469261 2469818 R secreted protein; function unknown. secreted protein.; hypothetical protein complement(2469261..2469818) Mycobacterium ulcerans Agy99 4550608 YP_906077.1 CDS MUL_2209 NC_008611.1 2470412 2471383 D cytoplasmic protein; thought to be involved in fatty acid degradation. FadB and FadA are the alpha and BetA subunits of the multifunctional enzyme complex of the fatty acid degradation cycle.; oxidoreductase 2470412..2471383 Mycobacterium ulcerans Agy99 4550609 YP_906078.1 CDS MUL_2210 NC_008611.1 2471577 2472005 D membrane protein; function unknown, unknown, contains a C-term cellobiohydrolase a (1,4-beta-cellobiosidase a) domain and a N-term cellulose-binding domain (CBD) domain. the CBD is found either at the N-term or at the C-term of endoglucanases, cellobiohydrolases (exoglucanases), or xylanases; chitinase/cellulase 2471577..2472005 Mycobacterium ulcerans Agy99 4550610 YP_906079.1 CDS MUL_2213 NC_008611.1 2474337 2474669 R cytoplasmic protein; could be involved in the catabolism of catechol to succinate- and acetyl-CoA in the beta-ketoadipate pathway (at the third step) [catalytic activity: 2,5-dihydro-5- oxofuran-2-acetate = 3,4-dihydro-5-oxofuran-2-acetate]; muconolactone isomerase complement(2474337..2474669) Mycobacterium ulcerans Agy99 4550613 YP_906080.1 CDS lpd NC_008611.1 2474844 2476238 D E3 component of alpha keto acid dehydrogenase complexes LpdC; forms a homodimer; binds one molecule of FAD monomer; catalyzes NAD+-dependent oxidation of dihydrolipoyl cofactors that are covalently linked to the E2 component; dihydrolipoamide dehydrogenase 2474844..2476238 Mycobacterium ulcerans Agy99 4550614 YP_906081.1 CDS MUL_2215 NC_008611.1 2476241 2476516 D membrane protein; hypothetical protein 2476241..2476516 Mycobacterium ulcerans Agy99 4550615 YP_906082.1 CDS MUL_2216 NC_008611.1 2476522 2476716 R cytoplasmic protein; hypothetical protein complement(2476522..2476716) Mycobacterium ulcerans Agy99 4550616 YP_906083.1 CDS MUL_2217 NC_008611.1 2476797 2477843 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(2476797..2477843) Mycobacterium ulcerans Agy99 4550617 YP_906084.1 CDS MUL_2219 NC_008611.1 2478359 2479693 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 2478359..2479693 Mycobacterium ulcerans Agy99 4550619 YP_906085.1 CDS MUL_2220 NC_008611.1 2480160 2480987 R secreted protein; function unknown. possible secreted protein.; hypothetical protein complement(2480160..2480987) Mycobacterium ulcerans Agy99 4550620 YP_906086.1 CDS MUL_2223 NC_008611.1 2482652 2483941 D cytoplasmic protein; hypothetical protein 2482652..2483941 Mycobacterium ulcerans Agy99 4550589 YP_906087.1 CDS MUL_2228 NC_008611.1 2486508 2486741 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein 2486508..2486741 Mycobacterium ulcerans Agy99 4550592 YP_906088.1 CDS MUL_2230 NC_008611.1 2487587 2488291 D cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 2487587..2488291 Mycobacterium ulcerans Agy99 4550594 YP_906089.1 CDS MUL_2231 NC_008611.1 2488316 2488633 D cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 2488316..2488633 Mycobacterium ulcerans Agy99 4550595 YP_906090.1 CDS MUL_2232 NC_008611.1 2488814 2489263 D Also detected in the extracellular matrix by proteomics, and in the membrane fraction.; cytoplasmic protein; function unknown. may be involved in a chaperoning process.; molecular chaperone 2488814..2489263 Mycobacterium ulcerans Agy99 4550596 YP_906091.1 CDS folE_1 NC_008611.1 2489971 2490417 D cytoplasmic protein; involved in the biosynthesis of tetrahydrofolate (at the first step) [catalytic activity: GTP + 2 H(2)O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3- trihydroxypropyl)DiHYD ro pteridine triphosphate]; GTP cyclohydrolase I FolE 2489971..2490417 Mycobacterium ulcerans Agy99 4550597 YP_906092.1 CDS MUL_2234 NC_008611.1 2490452 2491339 R Detected in the membrane fraction by proteomics (LC- MS/MS) Also detected in the cytoplasmic and extracellular matrix fractions by proteomics.; membrane protein; hypothetical protein complement(2490452..2491339) Mycobacterium ulcerans Agy99 4550598 YP_906093.1 CDS MUL_2236 NC_008611.1 2491790 2492674 R membrane protein; function unknown. possibly involved in signal transduction mechanisms.; hypothetical protein complement(2491790..2492674) Mycobacterium ulcerans Agy99 4550600 YP_906094.1 CDS MUL_2237 NC_008611.1 2492843 2493109 R cytoplasmic protein; hypothetical protein complement(2492843..2493109) Mycobacterium ulcerans Agy99 4550601 YP_906095.1 CDS MUL_2238 NC_008611.1 2493106 2493558 R cytoplasmic protein; the sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released.; alternative RNA polymerase sigma factor complement(2493106..2493558) Mycobacterium ulcerans Agy99 4550602 YP_906096.1 CDS MUL_2239 NC_008611.1 2493741 2494823 R membrane protein; function unknown. possibly involved in lipid metabolism.; hypothetical protein complement(2493741..2494823) Mycobacterium ulcerans Agy99 4550603 YP_906097.1 CDS MUL_2241 NC_008611.1 2494995 2495519 R cytoplasmic protein; hypothetical protein complement(2494995..2495519) Mycobacterium ulcerans Agy99 4550604 YP_906098.1 CDS MUL_2242 NC_008611.1 2495678 2495869 D hypothetical protein 2495678..2495869 Mycobacterium ulcerans Agy99 4550605 YP_906099.1 CDS esxR NC_008611.1 2495925 2496215 R secreted protein; EsaT-6 family protein complement(2495925..2496215) Mycobacterium ulcerans Agy99 4550570 YP_906100.1 CDS MUL_2245 NC_008611.1 2496583 2497869 R membrane protein; PPE family protein complement(2496583..2497869) Mycobacterium ulcerans Agy99 4550572 YP_906101.1 CDS MUL_2246 NC_008611.1 2497880 2498185 R secreted protein; PE family protein complement(2497880..2498185) Mycobacterium ulcerans Agy99 4550573 YP_906102.1 CDS MUL_2247 NC_008611.1 2498760 2499422 R membrane protein; PPE family protein complement(2498760..2499422) Mycobacterium ulcerans Agy99 4550574 YP_906103.1 CDS MUL_2249 NC_008611.1 2501416 2502252 R cytoplasmic protein; hypothetical protein complement(2501416..2502252) Mycobacterium ulcerans Agy99 4550576 YP_906104.1 CDS MUL_2250 NC_008611.1 2502459 2504369 R membrane protein; PE-PGRS family protein complement(2502459..2504369) Mycobacterium ulcerans Agy99 4550577 YP_906105.1 CDS MUL_2251 NC_008611.1 2504532 2505497 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(2504532..2505497) Mycobacterium ulcerans Agy99 4550578 YP_906106.1 CDS MUL_2252 NC_008611.1 2505600 2506670 R membrane protein; thought to be involved in active transport of sugar across the membrane (import) responsible for energy coupling to the transport system.; sugar-transport ATP-binding protein ABC transporter complement(2505600..2506670) Mycobacterium ulcerans Agy99 4550579 YP_906107.1 CDS MUL_2253 NC_008611.1 2506676 2507518 R membrane protein; sugar ABC transporter complement(2506676..2507518) Mycobacterium ulcerans Agy99 4550580 YP_906108.1 CDS MUL_2254 NC_008611.1 2507505 2508458 R membrane protein; thought to be involved in active transport of sugar across the membrane (import) responsible for the translocation of the substrate across the membrane.; sugar ABC transporter integral membrane protein complement(2507505..2508458) Mycobacterium ulcerans Agy99 4550581 YP_906109.1 CDS pncA NC_008611.1 2510652 2511212 R cytoplasmic protein; converts amides such as nicotinamide to corresponding acid.; pyrazinamidase/nicotinamidase, PncA complement(2510652..2511212) Mycobacterium ulcerans Agy99 4550584 YP_906110.1 CDS MUL_2258 NC_008611.1 2511308 2512123 D membrane protein; hypothetical protein 2511308..2512123 Mycobacterium ulcerans Agy99 4550585 YP_906111.1 CDS MUL_2259 NC_008611.1 2512120 2512635 R membrane protein; function unknown, in general gluconate kinase catalyzes the phosphoryl transfer from ATP to gluconate. the resulting product gluconate-6-phosphate is an important precursor of gluconate metabolism.; gluconate kinase complement(2512120..2512635) Mycobacterium ulcerans Agy99 4550586 YP_906112.1 CDS lipT NC_008611.1 2514633 2516138 R cytoplasmic protein; converts unknown esters to corresponding free acid and alcohol; carboxylesterase, LipT complement(2514633..2516138) Mycobacterium ulcerans Agy99 4550588 YP_906113.1 CDS lppI NC_008611.1 2516217 2516897 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; lipoprotein LppI 2516217..2516897 Mycobacterium ulcerans Agy99 4550538 YP_906114.1 CDS MUL_2263 NC_008611.1 2517227 2517544 D cytoplasmic protein; hypothetical protein 2517227..2517544 Mycobacterium ulcerans Agy99 4550539 YP_906115.1 CDS MUL_2264 NC_008611.1 2518033 2518413 D membrane protein; hypothetical protein 2518033..2518413 Mycobacterium ulcerans Agy99 4550540 YP_906116.1 CDS MUL_2265 NC_008611.1 2518555 2521215 R Also detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; hypothetical protein complement(2518555..2521215) Mycobacterium ulcerans Agy99 4550541 YP_906117.1 CDS pks12 NC_008611.1 2521288 2533863 R cytoplasmic protein; required for mycoketide synthesis.; polyketide synthase complement(2521288..2533863) Mycobacterium ulcerans Agy99 4550542 YP_906118.1 CDS MUL_2267 NC_008611.1 2534194 2534553 R cytoplasmic protein; hypothetical protein complement(2534194..2534553) Mycobacterium ulcerans Agy99 4550543 YP_906119.1 CDS MUL_2268 NC_008611.1 2534710 2535045 D cytoplasmic protein; hypothetical protein 2534710..2535045 Mycobacterium ulcerans Agy99 4550544 YP_906120.1 CDS ppm1B NC_008611.1 2535066 2535872 R This gene in two parts compared with MTB orthologue. Potential pseudogene but given same arrangement in M. marinum then this possibility seems less likely.; cytoplasmic protein; transfers mannose from GDP-mannose to all endogenous polyprenol-phosphates in mycobacterium tuberculosis; C-term polyprenol-monophosphomannose synthase Ppm1B complement(2535066..2535872) Mycobacterium ulcerans Agy99 4550545 YP_906121.1 CDS ppm1A NC_008611.1 2535898 2537715 R cytoplasmic protein; transfers mannose from GDP-mannose to all endogenous polyprenol-phosphates in mycobacterium tuberculosis; N-term polyprenol-monophosphomannose synthase Ppm1A complement(2535898..2537715) Mycobacterium ulcerans Agy99 4550546 YP_906122.1 CDS MUL_2270 NC_008611.1 2538005 2539609 R domain identity with predicted metal-dependent hydrolases with the TIM-barrel folds; hypothetical protein complement(2538005..2539609) Mycobacterium ulcerans Agy99 4550547 YP_906123.1 CDS fxsA NC_008611.1 2539640 2540272 R F exclusion of bacteriophage T7; overproduction of this protein in Escherichia coli inhibits the F plasmid-mediated exclusion of bacteriophage T7; interacts with the F plasmid-encoded PifA protein; inner membrane protein; FxsA protein complement(2539640..2540272) Mycobacterium ulcerans Agy99 4550548 YP_906124.1 CDS MUL_2272 NC_008611.1 2540345 2541055 D cytoplasmic protein; domain identity with dienelactone hydrolases; hypothetical protein 2540345..2541055 Mycobacterium ulcerans Agy99 4550549 YP_906125.1 CDS MUL_2273 NC_008611.1 2541330 2542934 D membrane protein; hypothetical protein 2541330..2542934 Mycobacterium ulcerans Agy99 4550550 YP_906126.1 CDS MUL_2274 NC_008611.1 2542931 2544538 R membrane protein; transmembrane protein complement(2542931..2544538) Mycobacterium ulcerans Agy99 4550551 YP_906127.1 CDS MUL_2275 NC_008611.1 2544663 2545541 R membrane protein; function unknown. domain identity to ThiJ-like intracellular proteases/amidases; hypothetical protein complement(2544663..2545541) Mycobacterium ulcerans Agy99 4550552 YP_906128.1 CDS MUL_2276 NC_008611.1 2545552 2545935 R cytoplasmic protein; hypothetical protein complement(2545552..2545935) Mycobacterium ulcerans Agy99 4550553 YP_906129.1 CDS cobN NC_008611.1 2545986 2549558 R with CobST catalyzes the formation of cobyrinic acid a,c-diamide from hydrogenobyrinic acid a,c-diamide in an ATP-dependent manner; involved in porphyrin and chlorophyll metabolism; vitamin B12 metabolism; cobaltochelatase subunit CobN complement(2545986..2549558) Mycobacterium ulcerans Agy99 4550554 YP_906130.1 CDS MUL_2278 NC_008611.1 2549551 2549772 R cytoplasmic protein; hypothetical protein complement(2549551..2549772) Mycobacterium ulcerans Agy99 4550555 YP_906131.1 CDS MUL_2279 NC_008611.1 2549838 2551469 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; membrane-bound oxidoreductase complement(2549838..2551469) Mycobacterium ulcerans Agy99 4550556 YP_906132.1 CDS MUL_2280 NC_008611.1 2551543 2551860 R cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 complement(2551543..2551860) Mycobacterium ulcerans Agy99 4550520 YP_906133.1 CDS MUL_2281 NC_008611.1 2551885 2552589 R cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 complement(2551885..2552589) Mycobacterium ulcerans Agy99 4550521 YP_906134.1 CDS MUL_2282 NC_008611.1 2552918 2553745 D cytoplasmic protein; cellular metabolism; short chain dehydrogenase 2552918..2553745 Mycobacterium ulcerans Agy99 4550522 YP_906135.1 CDS MUL_2283 NC_008611.1 2553750 2554637 D cytoplasmic protein; hypothetical protein 2553750..2554637 Mycobacterium ulcerans Agy99 4550523 YP_906136.1 CDS MUL_2284 NC_008611.1 2554774 2555394 R cytoplasmic protein; function unknown. contains adenylate cyclase domain; hypothetical protein complement(2554774..2555394) Mycobacterium ulcerans Agy99 4550524 YP_906137.1 CDS MUL_2288 NC_008611.1 2558891 2560000 D cobalamin biosynthesis protein 2558891..2560000 Mycobacterium ulcerans Agy99 4550526 YP_906138.1 CDS cobH NC_008611.1 2560018 2560644 D catalyzes the interconversion of precorrin-8X and hydrogenobyrinate; precorrin-8X methylmutase 2560018..2560644 Mycobacterium ulcerans Agy99 4550527 YP_906139.1 CDS cobI NC_008611.1 2560641 2562110 D detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; involved in cobalamin biosynthesis; bifunctional protein, CobI-CobJ fusion protein 2560641..2562110 Mycobacterium ulcerans Agy99 4550528 YP_906140.1 CDS MUL_2291 NC_008611.1 2562107 2563333 R cytoplasmic protein; hypothetical protein complement(2562107..2563333) Mycobacterium ulcerans Agy99 4550529 YP_906141.1 CDS mmpS4_1 NC_008611.1 2566357 2566788 R membrane protein; membrane transporter complement(2566357..2566788) Mycobacterium ulcerans Agy99 4550531 YP_906142.1 CDS blaC NC_008611.1 2567140 2568075 R Also detected in the cytoplasmic fraction by proteomics; membrane protein; hydrolyses beta-lactams to generate corresponding beta-amino acid [catalytic activity: a beta-lactam + H(2)O = a substituted beta-amino acid]; class a beta-lactamase, BlaC complement(2567140..2568075) Mycobacterium ulcerans Agy99 4550532 YP_906143.1 CDS sigC NC_008611.1 2568205 2568765 D Member of the extracytoplasmic function sigma factors which are active under specific conditions; binds with the catalytic core of RNA polymerase to produce the holoenzyme and directs bacterial core RNA polymerase to specific promoter elements to initiate transcription; RNA polymerase sigma factor SigC 2568205..2568765 Mycobacterium ulcerans Agy99 4550533 YP_906144.1 CDS cobK NC_008611.1 2568771 2569499 R CobK/CbiJ; there are 2 pathways for cobalamin (vitamin B12) production, one aerobic (ex. P. denitrificans), the other anaerobic (ex. S. typhimurium); the CobK/CbiJ perform similar reactions in both; the anaerobic pathway includes the use of a chelated cobalt ion in order for ring contraction to occur; CobK thus converts precorrin 6 into dihydro-precorrin 6 while CbiJ converts cobalt-precorrin 6 into cobalt-deihydro-precorrin 6; cobalt-precorrin-6x reductase complement(2568771..2569499) Mycobacterium ulcerans Agy99 4550534 YP_906145.1 CDS cobM NC_008611.1 2569496 2570245 R cytoplasmic protein; involved in cobalamin biosynthesis; precorrin-4 C11-methyltransferase, CobM complement(2569496..2570245) Mycobacterium ulcerans Agy99 4550535 YP_906146.1 CDS cobL NC_008611.1 2570242 2571414 R cytoplasmic protein; precorrin-6y methyltransferase, CobL complement(2570242..2571414) Mycobacterium ulcerans Agy99 4550536 YP_906147.1 CDS MUL_2299 NC_008611.1 2571411 2572160 R cytoplasmic protein; short chain dehydrogenase complement(2571411..2572160) Mycobacterium ulcerans Agy99 4550537 YP_906148.1 CDS MUL_2300 NC_008611.1 2572193 2572606 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (LC-MS/MS); cytoplasmic protein; hypothetical protein 2572193..2572606 Mycobacterium ulcerans Agy99 4550483 YP_906149.1 CDS MUL_2303 NC_008611.1 2574349 2575395 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 2574349..2575395 Mycobacterium ulcerans Agy99 4550486 YP_906150.1 CDS MUL_2304 NC_008611.1 2576340 2577185 R membrane protein; hypothetical protein complement(2576340..2577185) Mycobacterium ulcerans Agy99 4550487 YP_906151.1 CDS MUL_2305 NC_008611.1 2577182 2579467 R cytoplasmic protein; hypothetical protein complement(2577182..2579467) Mycobacterium ulcerans Agy99 4550488 YP_906152.1 CDS MUL_2306 NC_008611.1 2580144 2580398 D cytoplasmic protein; transposition of an insertion sequence; transposase 2580144..2580398 Mycobacterium ulcerans Agy99 4550489 YP_906153.1 CDS MUL_2307 NC_008611.1 2580689 2581201 D secreted protein; hypothetical protein 2580689..2581201 Mycobacterium ulcerans Agy99 4550490 YP_906154.1 CDS MUL_2308 NC_008611.1 2581255 2581704 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; hypothetical protein 2581255..2581704 Mycobacterium ulcerans Agy99 4550491 YP_906155.1 CDS MUL_2309 NC_008611.1 2582380 2582889 D membrane protein; hypothetical protein 2582380..2582889 Mycobacterium ulcerans Agy99 4550492 YP_906156.1 CDS MUL_2312 NC_008611.1 2585051 2586019 D cytoplasmic protein; hypothetical protein 2585051..2586019 Mycobacterium ulcerans Agy99 4550494 YP_906157.1 CDS MUL_2313 NC_008611.1 2586116 2589457 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(2586116..2589457) Mycobacterium ulcerans Agy99 4550495 YP_906158.1 CDS MUL_2314 NC_008611.1 2589450 2590145 R cytoplasmic protein; hypothetical protein complement(2589450..2590145) Mycobacterium ulcerans Agy99 4550496 YP_906159.1 CDS MUL_2315 NC_008611.1 2590186 2591625 R cytoplasmic protein; hypothetical protein complement(2590186..2591625) Mycobacterium ulcerans Agy99 4550497 YP_906160.1 CDS MUL_2317 NC_008611.1 2592285 2592548 R cytoplasmic protein; hypothetical protein complement(2592285..2592548) Mycobacterium ulcerans Agy99 4550498 YP_906161.1 CDS MUL_2318 NC_008611.1 2592555 2595149 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(2592555..2595149) Mycobacterium ulcerans Agy99 4550499 YP_906162.1 CDS MUL_2320 NC_008611.1 2595346 2597589 R cytoplasmic protein; hypothetical protein complement(2595346..2597589) Mycobacterium ulcerans Agy99 4550465 YP_906163.1 CDS pepE NC_008611.1 2597626 2598753 R Detected in the cytoplamic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hydrolysis of peptide bonds; dipeptidase PepE complement(2597626..2598753) Mycobacterium ulcerans Agy99 4550466 YP_906164.1 CDS MUL_2322 NC_008611.1 2598877 2599833 D cytoplasmic protein; involved in DNA metabolism; 5'-3' exonuclease 2598877..2599833 Mycobacterium ulcerans Agy99 4550467 YP_906165.1 CDS MUL_2323 NC_008611.1 2599846 2600610 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; hypothetical protein complement(2599846..2600610) Mycobacterium ulcerans Agy99 4550468 YP_906166.1 CDS helY NC_008611.1 2600717 2603473 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; DNA helicase activity.; ATP-dependent DNA helicase HelY complement(2600717..2603473) Mycobacterium ulcerans Agy99 4550469 YP_906167.1 CDS tatC NC_008611.1 2603561 2604511 R membrane protein; involved in proteins export: required for correct localization of precursor proteins bearing signal peptides with the twin arginine conserved motif S/T-R-R-X-F-L-K. this sec-independent pathway is termed tat for twin- arginine translocation system.; sec-independent protein translocase transmembrane protein TatC complement(2603561..2604511) Mycobacterium ulcerans Agy99 4550470 YP_906168.1 CDS tatA NC_008611.1 2604557 2604823 R TatA; similar to TatE that is found in some proteobacteria; part of system that translocates proteins with a conserved twin arginine motif across the inner membrane; capable of translocating folded substrates typically those with bound cofactors; similar to a protein import system in thylakoid membranes; twin arginine translocase protein A complement(2604557..2604823) Mycobacterium ulcerans Agy99 4550471 YP_906169.1 CDS MUL_2327 NC_008611.1 2604950 2605918 R cytoplasmic protein; hypothetical protein complement(2604950..2605918) Mycobacterium ulcerans Agy99 4550472 YP_906170.1 CDS MUL_2328 NC_008611.1 2605968 2606966 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; contains domain homology to transcriptional regulators; hypothetical protein complement(2605968..2606966) Mycobacterium ulcerans Agy99 4550473 YP_906171.1 CDS MUL_2329 NC_008611.1 2607020 2608378 R cytoplasmic protein; hypothetical protein complement(2607020..2608378) Mycobacterium ulcerans Agy99 4550474 YP_906172.1 CDS prcA NC_008611.1 2608436 2609239 R Detected in the cytoplasmic, membrane and secreted protein fractions by proteomics.; cytoplasmic protein; protein degradation; proteasome PrcA complement(2608436..2609239) Mycobacterium ulcerans Agy99 4550475 YP_906173.1 CDS prcB NC_008611.1 2609236 2610105 R Detected in the cytoplasmic, the membrane and the secreted fractions by proteomics.; cytoplasmic protein; protein degradation; proteasome PrcB complement(2609236..2610105) Mycobacterium ulcerans Agy99 4550476 YP_906174.1 CDS MUL_2332 NC_008611.1 2610102 2610296 R Detected in the cytoplasmic ans secreted fractions by 2D-LC-MS/MS.; cytoplasmic protein; hypothetical protein complement(2610102..2610296) Mycobacterium ulcerans Agy99 4550477 YP_906175.1 CDS MUL_2333 NC_008611.1 2610468 2611976 R Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); cytoplasmic protein; hypothetical protein complement(2610468..2611976) Mycobacterium ulcerans Agy99 4550478 YP_906176.1 CDS MUL_2334 NC_008611.1 2612273 2613370 D Detected in the cytoplasmic fraction by proteomics.; cytoplasmic protein; hypothetical protein 2612273..2613370 Mycobacterium ulcerans Agy99 4550479 YP_906177.1 CDS MUL_2335 NC_008611.1 2613578 2614546 D membrane protein; integral membrane protein 2613578..2614546 Mycobacterium ulcerans Agy99 4550480 YP_906178.1 CDS MUL_2336 NC_008611.1 2614607 2615182 D cytoplasmic protein; hypothetical protein 2614607..2615182 Mycobacterium ulcerans Agy99 4550481 YP_906179.1 CDS MUL_2337 NC_008611.1 2615204 2617033 R cytoplasmic protein; ATPase complement(2615204..2617033) Mycobacterium ulcerans Agy99 4550482 YP_906180.1 CDS lppK NC_008611.1 2617331 2617990 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; lipoprotein LppK 2617331..2617990 Mycobacterium ulcerans Agy99 4550450 YP_906181.1 CDS MUL_2339 NC_008611.1 2617999 2618301 D cytoplasmic protein; hypothetical protein 2617999..2618301 Mycobacterium ulcerans Agy99 4550451 YP_906182.1 CDS MUL_2340 NC_008611.1 2618093 2619157 R cytoplasmic protein; involved in transfer of methyl group (from S- adenosyl-L-methionine to a substrate); RNA methyltransferase complement(2618093..2619157) Mycobacterium ulcerans Agy99 4550452 YP_906183.1 CDS MUL_2341 NC_008611.1 2619231 2620112 D cytoplasmic protein; has significant domain identity to RecB family exonucleases; hypothetical protein 2619231..2620112 Mycobacterium ulcerans Agy99 4550453 YP_906184.1 CDS MUL_2342 NC_008611.1 2620109 2620888 D cytoplasmic protein; hypothetical protein 2620109..2620888 Mycobacterium ulcerans Agy99 4550454 YP_906185.1 CDS MUL_2344 NC_008611.1 2621540 2622586 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 2621540..2622586 Mycobacterium ulcerans Agy99 4550456 YP_906186.1 CDS MUL_2347 NC_008611.1 2626438 2627772 R cytoplasmic protein; required for the transposition of the insertion element IS2606; transposase for IS2606 complement(2626438..2627772) Mycobacterium ulcerans Agy99 4550457 YP_906187.1 CDS MUL_2350 NC_008611.1 2636142 2637476 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 2636142..2637476 Mycobacterium ulcerans Agy99 4550460 YP_906188.1 CDS MUL_2351 NC_008611.1 2637929 2638975 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 2637929..2638975 Mycobacterium ulcerans Agy99 4550461 YP_906189.1 CDS hisG NC_008611.1 2639255 2640115 R long form of enzyme; catalyzes the formation of N'-5'-phosphoribosyl-ATP from phosphoribosyl pyrophosphate; crucial role in histidine biosynthesis; forms active dimers and inactive hexamers which is dependent on concentration of substrates and inhibitors; ATP phosphoribosyltransferase complement(2639255..2640115) Mycobacterium ulcerans Agy99 4550462 YP_906190.1 CDS hisE NC_008611.1 2640112 2640393 R catalyzes the formation of 1-(5-phosphoribosyl)-AMP from 1-(5-phosphoribolsyl)-ATP in histidine biosynthesis; phosphoribosyl-ATP pyrophosphatase complement(2640112..2640393) Mycobacterium ulcerans Agy99 4550463 YP_906191.1 CDS MUL_2354 NC_008611.1 2640512 2642440 R membrane protein; PE-PGRS family protein complement(2640512..2642440) Mycobacterium ulcerans Agy99 4550464 YP_906192.1 CDS MUL_2357 NC_008611.1 2644847 2645725 D cytoplasmic protein; hypothetical protein 2644847..2645725 Mycobacterium ulcerans Agy99 4550433 YP_906193.1 CDS MUL_2358 NC_008611.1 2645863 2646984 R membrane protein; function unknown, probably involved in cellular metabolism.; FAD-dependent oxidoreductase complement(2645863..2646984) Mycobacterium ulcerans Agy99 4550434 YP_906194.1 CDS MUL_2359 NC_008611.1 2647018 2647899 R cytoplasmic protein; short chain dehydrogenase complement(2647018..2647899) Mycobacterium ulcerans Agy99 4550435 YP_906195.1 CDS mshC NC_008611.1 2647923 2649158 R catalyzes a two-step reaction; charges a cysteine by linking its carboxyl group to the alpha-phosphate of ATP then transfers the aminoacyl-adenylate to its tRNA; cysteinyl-tRNA synthetase complement(2647923..2649158) Mycobacterium ulcerans Agy99 4550436 YP_906196.1 CDS gabD1_1 NC_008611.1 2649428 2651044 D cytoplasmic protein; involved in 4-aminobutyrate (GabA) degradation pathway [catalytic activity: succinate semialdehyde + NAD(P)(+) + H(2)O = succinate + NAD(P)H]; succinate-semialdehyde dehydrogenase [NADP+] dependent, GabD1 2649428..2651044 Mycobacterium ulcerans Agy99 4550437 YP_906197.1 CDS cysQ NC_008611.1 2651073 2651864 R cytoplasmic protein; may help control the pool of 3'-phosphoadenoside 5'- phosphosulfate, or its use in sulfite synthesis (by similarity); monophosphatase CysQ complement(2651073..2651864) Mycobacterium ulcerans Agy99 4550438 YP_906198.1 CDS MUL_2363 NC_008611.1 2651976 2652761 R cytoplasmic protein; hypothetical protein complement(2651976..2652761) Mycobacterium ulcerans Agy99 4550439 YP_906199.1 CDS MUL_2364 NC_008611.1 2652775 2653362 R Detected in the cytoplasmic and secreted fractions by 2D-LC-MS/MS.; cytoplasmic protein; hypothetical protein complement(2652775..2653362) Mycobacterium ulcerans Agy99 4550440 YP_906200.1 CDS MUL_2365 NC_008611.1 2653451 2654197 R cytoplasmic protein; hypothetical protein complement(2653451..2654197) Mycobacterium ulcerans Agy99 4550441 YP_906201.1 CDS MUL_2366 NC_008611.1 2654194 2655024 R BacA; phosphatase activity in Escherichia coli not kinase; involved in bacitracin resistance as bacitracin supposedly sequesters undecaprenyl disphosphate which reduces the pool of lipid carrier available to the cell; undecaprenyl pyrophosphate phosphatase complement(2654194..2655024) Mycobacterium ulcerans Agy99 4550442 YP_906202.1 CDS MUL_2367 NC_008611.1 2655073 2655453 R cytoplasmic protein; hypothetical protein complement(2655073..2655453) Mycobacterium ulcerans Agy99 4550443 YP_906203.1 CDS lppL NC_008611.1 2655513 2656583 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; lipoprotein LppL 2655513..2656583 Mycobacterium ulcerans Agy99 4550444 YP_906204.1 CDS MUL_2369 NC_008611.1 2656580 2656843 D cytoplasmic protein; hypothetical protein 2656580..2656843 Mycobacterium ulcerans Agy99 4550445 YP_906205.1 CDS pyrD NC_008611.1 2656876 2657958 D catalyzes the conversion of dihydroorotate to orotate in the pyrimidine biosynthesis pathway; uses a flavin nucleotide as an essential cofactor; class 2 enzymes are monomeric and compared to the class 1 class 2 possess an extended N terminus, which plays a role in the membrane association of the enzyme and provides the binding site for the respiratory quinones that serve as physiological electron acceptors; dihydroorotate dehydrogenase 2 2656876..2657958 Mycobacterium ulcerans Agy99 4550446 YP_906206.1 CDS MUL_2371 NC_008611.1 2657970 2659358 R cytoplasmic protein; hypothetical protein complement(2657970..2659358) Mycobacterium ulcerans Agy99 4550447 YP_906207.1 CDS MUL_2372 NC_008611.1 2659485 2660012 R Also detected in the extracellular matrix by proteomics.; cytoplasmic protein; function unknown but contains bacterial/archael phosphatidylethanolamine-binding (PEBP) domain; hypothetical protein complement(2659485..2660012) Mycobacterium ulcerans Agy99 4550448 YP_906208.1 CDS MUL_2373 NC_008611.1 2660039 2661394 R Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); cytoplasmic protein; function unknown - but has domain identity with cetylornithine deacetylase/succinyl-diaminopimelate desuccinylase and related deacylases [amino acid transport and metabolism]; hypothetical protein complement(2660039..2661394) Mycobacterium ulcerans Agy99 4550449 YP_906209.1 CDS MUL_2376 NC_008611.1 2662592 2663041 D cytoplasmic protein; hypothetical protein 2662592..2663041 Mycobacterium ulcerans Agy99 4550416 YP_906210.1 CDS MUL_2377 NC_008611.1 2662972 2663787 R cytoplasmic protein; orthologue in M. tuberculosis H37Rv has been shown to catalyze the transfer of a methyl group onto the lipid moiety of phthiotriol dimycocerosates to form DIM.; methyltransferase complement(2662972..2663787) Mycobacterium ulcerans Agy99 4550417 YP_906211.1 CDS MUL_2379 NC_008611.1 2664299 2664529 R cytoplasmic protein; hypothetical protein complement(2664299..2664529) Mycobacterium ulcerans Agy99 4550419 YP_906212.1 CDS mbtH NC_008611.1 2665397 2665627 R cytoplasmic protein; thought to be involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins.; protein MbtH complement(2665397..2665627) Mycobacterium ulcerans Agy99 4550421 YP_906213.1 CDS mmpS4 NC_008611.1 2668786 2669256 R membrane protein; membrane protein MmpS4 complement(2668786..2669256) Mycobacterium ulcerans Agy99 4550423 YP_906214.1 CDS MUL_2384 NC_008611.1 2669561 2670607 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 2669561..2670607 Mycobacterium ulcerans Agy99 4550424 YP_906215.1 CDS MUL_2386 NC_008611.1 2671459 2671980 D Detected in the membrane fraction by proteomics.; secreted protein; hypothetical protein 2671459..2671980 Mycobacterium ulcerans Agy99 4550426 YP_906216.1 CDS MUL_2387 NC_008611.1 2672027 2672707 R cytoplasmic protein; hypothetical protein complement(2672027..2672707) Mycobacterium ulcerans Agy99 4550427 YP_906217.1 CDS MUL_2388 NC_008611.1 2672923 2673540 D hypothetical protein 2672923..2673540 Mycobacterium ulcerans Agy99 4550428 YP_906218.1 CDS MUL_2389 NC_008611.1 2673544 2674269 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 2673544..2674269 Mycobacterium ulcerans Agy99 4550429 YP_906219.1 CDS MUL_2390 NC_008611.1 2674336 2675202 D cytoplasmic protein; hypothetical protein 2674336..2675202 Mycobacterium ulcerans Agy99 4550430 YP_906220.1 CDS MUL_2391 NC_008611.1 2675246 2675947 R membrane protein; hypothetical protein complement(2675246..2675947) Mycobacterium ulcerans Agy99 4550367 YP_906221.1 CDS MUL_2392 NC_008611.1 2676195 2676458 D membrane protein; hypothetical protein 2676195..2676458 Mycobacterium ulcerans Agy99 4550368 YP_906222.1 CDS MUL_2393 NC_008611.1 2676498 2676851 R cytoplasmic protein; hypothetical protein complement(2676498..2676851) Mycobacterium ulcerans Agy99 4550369 YP_906223.1 CDS MUL_2394 NC_008611.1 2676950 2677177 D cytoplasmic protein; hypothetical protein 2676950..2677177 Mycobacterium ulcerans Agy99 4550370 YP_906224.1 CDS MUL_2395 NC_008611.1 2677467 2678669 D PE26; membrane protein; PE family protein 2677467..2678669 Mycobacterium ulcerans Agy99 4550371 YP_906225.1 CDS MUL_2397 NC_008611.1 2679184 2680029 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics.; cytoplasmic protein; hypothetical protein complement(2679184..2680029) Mycobacterium ulcerans Agy99 4550373 YP_906226.1 CDS MUL_2398 NC_008611.1 2680007 2681086 R membrane protein; hypothetical protein complement(2680007..2681086) Mycobacterium ulcerans Agy99 4550374 YP_906227.1 CDS MUL_2399 NC_008611.1 2681083 2682324 R membrane protein; ATPase; possibly catalyzes the transport of undeterminated substrate with hydrolysis of ATP [catalytic activity: ATP + H(2)O + undeterminated substrate(in) = ADP + phosphate + undeterminated substrate(out)]; ATPase complement(2681083..2682324) Mycobacterium ulcerans Agy99 4550375 YP_906228.1 CDS MUL_2401 NC_008611.1 2684249 2685295 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(2684249..2685295) Mycobacterium ulcerans Agy99 4550377 YP_906229.1 CDS MUL_2404 NC_008611.1 2687010 2688341 D cytoplasmic protein; required for the transposition of the insertion element IS2606; transposase for IS2606 2687010..2688341 Mycobacterium ulcerans Agy99 4550379 YP_906230.1 CDS MUL_2405 NC_008611.1 2688390 2689436 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(2688390..2689436) Mycobacterium ulcerans Agy99 4550380 YP_906231.1 CDS smpB NC_008611.1 2690078 2690584 R binds to ssrA RNA (tmRNA) and is required for its successful binding to ribosomes; also appears to function in the trans-translation step by promoting accommodation of tmRNA into the ribosomal A site; SmpB protects the tmRNA from RNase R degradation in Caulobacter crescentus; both the tmRNA and SmpB are regulated in cell cycle-dependent manner; functions in release of stalled ribosomes from damaged mRNAs and targeting proteins for degradation; SsrA-binding protein complement(2690078..2690584) Mycobacterium ulcerans Agy99 4550381 YP_906232.1 CDS ftsX NC_008611.1 2690587 2691480 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in growth (principally during log phase cells) thought to be involved in active transport of septation component across the membrane. responsible for the translocation of the substrate across the membrane. coded for in an operon essential for ce; cell division protein FtsX complement(2690587..2691480) Mycobacterium ulcerans Agy99 4550382 YP_906233.1 CDS ftsE NC_008611.1 2691481 2692170 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in growth. thought to be involved in active transport of septation component across the membrane. responsible for energy coupling to the transport system. coded for in an operon essential for cell division.; cell division ATP-binding protein FtsE complement(2691481..2692170) Mycobacterium ulcerans Agy99 4550350 YP_906234.1 CDS MUL_2410 NC_008611.1 2692215 2692715 R membrane protein; function unknown: predicted solute binding protein; hypothetical protein complement(2692215..2692715) Mycobacterium ulcerans Agy99 4550351 YP_906235.1 CDS MUL_2411 NC_008611.1 2692718 2693704 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown but contains mechanosensitive (ms) ion channel forming domain with a possible role in cell envelope biogenesis.; transmembrane protein complement(2692718..2693704) Mycobacterium ulcerans Agy99 4550352 YP_906236.1 CDS prfB NC_008611.1 2693694 2694809 R recognizes the termination signals UGA and UAA during protein translation a specificity which is dependent on amino acid residues residing in loops of the L-shaped tRNA-like molecule of RF2; in some organisms control of PrfB protein levels is maintained through a +1 ribosomal frameshifting mechanism; this protein is similar to release factor 1; peptide chain release factor 2 complement(2693694..2694809) Mycobacterium ulcerans Agy99 4550353 YP_906237.1 CDS fprA_1 NC_008611.1 2694868 2696313 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics.; cytoplasmic protein; generates oxidized ferredoxin from ferredoxin [catalytic activity: reduced ferredoxin + NADP(+) = oxidized ferredoxin + NADPH]; NADPH:adrenodoxin oxidoreductase FprA 2694868..2696313 Mycobacterium ulcerans Agy99 4550354 YP_906238.1 CDS MUL_2414 NC_008611.1 2696898 2697944 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 2696898..2697944 Mycobacterium ulcerans Agy99 4550355 YP_906239.1 CDS MUL_2416 NC_008611.1 2699261 2700865 R cytoplasmic protein; hypothetical protein complement(2699261..2700865) Mycobacterium ulcerans Agy99 4550357 YP_906240.1 CDS MUL_2417 NC_008611.1 2701011 2702018 D cytoplasmic protein; hypothetical protein 2701011..2702018 Mycobacterium ulcerans Agy99 4550358 YP_906241.1 CDS MUL_2418 NC_008611.1 2702023 2702451 D Also detected in the extracellular matrix and the membrane fraction by proteomics.; cytoplasmic protein; hypothetical protein 2702023..2702451 Mycobacterium ulcerans Agy99 4550359 YP_906242.1 CDS MUL_2419 NC_008611.1 2702536 2702958 R cytoplasmic protein; hypothetical protein complement(2702536..2702958) Mycobacterium ulcerans Agy99 4550360 YP_906243.1 CDS MUL_2420 NC_008611.1 2702955 2704343 R cytoplasmic protein; hypothetical protein complement(2702955..2704343) Mycobacterium ulcerans Agy99 4550361 YP_906244.1 CDS MUL_2421 NC_008611.1 2704487 2705533 D cytoplasmic protein; hypothetical protein 2704487..2705533 Mycobacterium ulcerans Agy99 4550362 YP_906245.1 CDS devS NC_008611.1 2705513 2707270 R membrane protein; sensor part of the two component regulatory system DevR/DevS. thought to control HspX|Acr expression.; two component sensor histidine kinase DevS complement(2705513..2707270) Mycobacterium ulcerans Agy99 4550363 YP_906246.1 CDS devR NC_008611.1 2707282 2707932 R cytoplasmic protein; regulator part of the two component regulatory system DevR/DevS. controls HspX|Acr expression.; two component transcriptional regulatory protein DevR complement(2707282..2707932) Mycobacterium ulcerans Agy99 4550364 YP_906247.1 CDS MUL_2424 NC_008611.1 2707956 2708750 R membrane protein; may play a role in the adaptation to hypoxia, participating in the phosphorelay in the two component regulatory system DevR/DevS; hypothetical protein complement(2707956..2708750) Mycobacterium ulcerans Agy99 4550365 YP_906248.1 CDS MUL_2425 NC_008611.1 2708926 2710260 R cytoplasmic protein; required for the transposition of the insertion element IS2606; transposase for IS2606 complement(2708926..2710260) Mycobacterium ulcerans Agy99 4550366 YP_906249.1 CDS MUL_2426 NC_008611.1 2710381 2711427 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(2710381..2711427) Mycobacterium ulcerans Agy99 4550333 YP_906250.1 CDS MUL_2427 NC_008611.1 2712510 2713655 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; PPE family protein 2712510..2713655 Mycobacterium ulcerans Agy99 4550334 YP_906251.1 CDS MUL_2428 NC_008611.1 2714196 2715344 D PPE51; membrane protein; PPE family protein 2714196..2715344 Mycobacterium ulcerans Agy99 4550335 YP_906252.1 CDS MUL_2429 NC_008611.1 2715363 2715698 R membrane protein; hypothetical protein complement(2715363..2715698) Mycobacterium ulcerans Agy99 4550336 YP_906253.1 CDS MUL_2430 NC_008611.1 2715722 2716510 D cytoplasmic protein; function unknown, involved in cellular metabolism.; monophosphatase 2715722..2716510 Mycobacterium ulcerans Agy99 4550337 YP_906254.1 CDS fadE24 NC_008611.1 2716592 2717989 D cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE24 2716592..2717989 Mycobacterium ulcerans Agy99 4550338 YP_906255.1 CDS fadE23 NC_008611.1 2718044 2719249 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE23 2718044..2719249 Mycobacterium ulcerans Agy99 4550339 YP_906256.1 CDS ilvB1_1 NC_008611.1 2719259 2720935 R catalyzes the formation of 2-acetolactate from pyruvate in stationary phase; acetolactate synthase complement(2719259..2720935) Mycobacterium ulcerans Agy99 4550340 YP_906257.1 CDS MUL_2434 NC_008611.1 2720932 2721708 R cytoplasmic protein; function unknown, possible role secondary metabolite biosynthesis; alpha-acetolactate decarboxylase complement(2720932..2721708) Mycobacterium ulcerans Agy99 4550341 YP_906258.1 CDS MUL_2435 NC_008611.1 2721757 2722791 R catalyzes the formation of pyruvate and acetaldehyde from 4-hydroxy-2-ketovaleric acid; involved in the degradation of phenylpropionate; 4-hydroxy-2-ketovalerate aldolase complement(2721757..2722791) Mycobacterium ulcerans Agy99 4550342 YP_906259.2 CDS mhpF NC_008611.1 2722788 2723735 R catalyzes the formation of acetyl-CoA from acetalaldehyde; acetaldehyde dehydrogenase complement(2722788..2723735) Mycobacterium ulcerans Agy99 4550343 YP_906260.1 CDS MUL_2437 NC_008611.1 2724109 2725344 D Potential extended N-term caused by an u/s DNA deletion and point mutation that has created a fusion of MON6244 and MON2437; membrane protein; hypothetical protein 2724109..2725344 Mycobacterium ulcerans Agy99 4550344 YP_906261.1 CDS MUL_2438 NC_008611.1 2725412 2726410 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 2725412..2726410 Mycobacterium ulcerans Agy99 4550345 YP_906262.1 CDS fadB4 NC_008611.1 2726453 2727424 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in lipid degradation [catalytic activity: NADPH + quinone = NADP(+) + semiquinone]; NADPH quinone oxidoreductase FadB4 2726453..2727424 Mycobacterium ulcerans Agy99 4550346 YP_906263.1 CDS MUL_2440 NC_008611.1 2727442 2729355 R PPE28; membrane protein; PPE family protein complement(2727442..2729355) Mycobacterium ulcerans Agy99 4550347 YP_906264.1 CDS MUL_2441 NC_008611.1 2729451 2730785 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 2729451..2730785 Mycobacterium ulcerans Agy99 4550348 YP_906265.1 CDS MUL_2442 NC_008611.1 2730856 2731902 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(2730856..2731902) Mycobacterium ulcerans Agy99 4550349 YP_906266.1 CDS MUL_2443 NC_008611.1 2732267 2733412 D cytoplasmic protein; oxidoreductase 2732267..2733412 Mycobacterium ulcerans Agy99 4550315 YP_906267.1 CDS MUL_2446 NC_008611.1 2734612 2735817 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein 2734612..2735817 Mycobacterium ulcerans Agy99 4550317 YP_906268.1 CDS MUL_2447 NC_008611.1 2735814 2736710 D cytoplasmic protein; hypothetical protein 2735814..2736710 Mycobacterium ulcerans Agy99 4550318 YP_906269.1 CDS MUL_2448 NC_008611.1 2736710 2737351 D cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein 2736710..2737351 Mycobacterium ulcerans Agy99 4550319 YP_906270.1 CDS MUL_2450 NC_008611.1 2738611 2739573 D cytoplasmic protein; hypothetical protein 2738611..2739573 Mycobacterium ulcerans Agy99 4550321 YP_906271.1 CDS MUL_5092 NC_008611.1 2739573 2739914 D cytoplasmic protein; hypothetical protein 2739573..2739914 Mycobacterium ulcerans Agy99 4550322 YP_906272.1 CDS MUL_2451 NC_008611.1 2739926 2740336 D cytoplasmic protein; hypothetical protein 2739926..2740336 Mycobacterium ulcerans Agy99 4550323 YP_906273.1 CDS MUL_2452 NC_008611.1 2740372 2740911 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 2740372..2740911 Mycobacterium ulcerans Agy99 4550324 YP_906274.1 CDS MUL_2453 NC_008611.1 2740986 2741387 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS; cytoplasmic protein; two-component system response regulator 2740986..2741387 Mycobacterium ulcerans Agy99 4550325 YP_906275.1 CDS MUL_2455 NC_008611.1 2742334 2743380 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(2742334..2743380) Mycobacterium ulcerans Agy99 4550327 YP_906276.1 CDS MUL_2457 NC_008611.1 2746024 2746728 D cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 2746024..2746728 Mycobacterium ulcerans Agy99 4550329 YP_906277.1 CDS MUL_2458 NC_008611.1 2746753 2747070 D cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 2746753..2747070 Mycobacterium ulcerans Agy99 4550330 YP_906278.1 CDS nuoA NC_008611.1 2747699 2748082 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit A 2747699..2748082 Mycobacterium ulcerans Agy99 4550331 YP_906279.1 CDS nuoB NC_008611.1 2748091 2748645 D The point of entry for the majority of electrons that traverse the respiratory chain eventually resulting in the reduction of oxygen; NADH dehydrogenase subunit B 2748091..2748645 Mycobacterium ulcerans Agy99 4550332 YP_906280.1 CDS nuoC NC_008611.1 2748642 2749358 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit C 2748642..2749358 Mycobacterium ulcerans Agy99 4550298 YP_906281.1 CDS nuoD NC_008611.1 2749378 2750676 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit D 2749378..2750676 Mycobacterium ulcerans Agy99 4550299 YP_906282.1 CDS nuoE NC_008611.1 2750673 2751428 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit E 2750673..2751428 Mycobacterium ulcerans Agy99 4550300 YP_906283.1 CDS nuoF NC_008611.1 2751428 2752774 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS); involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]; NADH dehydrogenase I subunit F 2751428..2752774 Mycobacterium ulcerans Agy99 4550301 YP_906284.1 CDS nuoG NC_008611.1 2752771 2755188 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit G 2752771..2755188 Mycobacterium ulcerans Agy99 4550302 YP_906285.1 CDS nuoH NC_008611.1 2755238 2756512 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit H 2755238..2756512 Mycobacterium ulcerans Agy99 4550303 YP_906286.1 CDS nuoI NC_008611.1 2756505 2757050 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit I 2756505..2757050 Mycobacterium ulcerans Agy99 4550304 YP_906287.1 CDS nuoJ NC_008611.1 2757047 2757835 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit J 2757047..2757835 Mycobacterium ulcerans Agy99 4550305 YP_906288.1 CDS nuoK NC_008611.1 2757832 2758131 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit K 2757832..2758131 Mycobacterium ulcerans Agy99 4550306 YP_906289.1 CDS nuoL NC_008611.1 2758142 2760043 D Catalyzes the transfer of electrons from NADH to ubiquinone; NADH dehydrogenase subunit L 2758142..2760043 Mycobacterium ulcerans Agy99 4550307 YP_906290.1 CDS nuoM NC_008611.1 2760037 2761626 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit M 2760037..2761626 Mycobacterium ulcerans Agy99 4550308 YP_906291.1 CDS nuoN NC_008611.1 2761623 2763209 D Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit N 2761623..2763209 Mycobacterium ulcerans Agy99 4550309 YP_906292.1 CDS MUL_2474 NC_008611.1 2764120 2765166 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 2764120..2765166 Mycobacterium ulcerans Agy99 4550311 YP_906293.1 CDS MUL_2475 NC_008611.1 2766481 2766930 R membrane protein; hypothetical protein complement(2766481..2766930) Mycobacterium ulcerans Agy99 4550312 YP_906294.1 CDS MUL_2476 NC_008611.1 2766932 2768209 R membrane protein; hypothetical protein complement(2766932..2768209) Mycobacterium ulcerans Agy99 4550313 YP_906295.1 CDS moxR3 NC_008611.1 2768223 2769191 R cytoplasmic protein; involved in transcriptional mechanism; regulates methanol dehydrogenase.; methanol dehydrogenase transcriptional regulatory protein MoxR3 complement(2768223..2769191) Mycobacterium ulcerans Agy99 4550314 YP_906296.1 CDS MUL_2480 NC_008611.1 2769717 2770697 R cytoplasmic protein; hypothetical protein complement(2769717..2770697) Mycobacterium ulcerans Agy99 4550261 YP_906297.1 CDS MUL_2481 NC_008611.1 2770814 2771575 R cytoplasmic protein; function unknown, possibly involved in cellular metabolism.; short-chain type dehydrogenase/reductase complement(2770814..2771575) Mycobacterium ulcerans Agy99 4550262 YP_906298.1 CDS MUL_2482 NC_008611.1 2771677 2772279 R cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator complement(2771677..2772279) Mycobacterium ulcerans Agy99 4550263 YP_906299.1 CDS MUL_2483 NC_008611.1 2772324 2773460 D cytoplasmic protein; hypothetical protein 2772324..2773460 Mycobacterium ulcerans Agy99 4550264 YP_906300.1 CDS MUL_2484 NC_008611.1 2773460 2774584 D cytoplasmic protein; hypothetical protein 2773460..2774584 Mycobacterium ulcerans Agy99 4550265 YP_906301.1 CDS MUL_2485 NC_008611.1 2774738 2776912 D PE_PGRS9; membrane protein; PE-PGRS family protein 2774738..2776912 Mycobacterium ulcerans Agy99 4550266 YP_906302.1 CDS MUL_2486 NC_008611.1 2776974 2777972 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase complement(2776974..2777972) Mycobacterium ulcerans Agy99 4550267 YP_906303.1 CDS aofH NC_008611.1 2778076 2779422 D cytoplasmic protein; may catalyze the oxidative deamination: oxidize on primary amines, and perhaps on secondary and tertiary amines [catalytic activity: RCH(2)NH(2) + H(2)O + O(2) = RchO + NH(3) + H(2)O(2)] may have important function in metabolism. supposed involved; flavin-containing monoamine oxidase AofH 2778076..2779422 Mycobacterium ulcerans Agy99 4550268 YP_906304.1 CDS hpx NC_008611.1 2779417 2780397 R cytoplasmic protein; supposed involved in detoxification reactions.; non-heme haloperoxidase Hpx complement(2779417..2780397) Mycobacterium ulcerans Agy99 4550269 YP_906305.1 CDS MUL_2489 NC_008611.1 2780638 2781948 D cytoplasmic protein; possibly catalyzes the oxidative deamination: oxidize on primary amines, and perhaps on secondary and tertiary amines [catalytic activity: RCH(2)NH(2) + H(2)O + O(2) = RchO + NH(3) + H(2)O(2)]; monoamine oxidase 2780638..2781948 Mycobacterium ulcerans Agy99 4550270 YP_906306.1 CDS MUL_5120 NC_008611.1 2781959 2782363 R cytoplasmic protein; hypothetical protein complement(2781959..2782363) Mycobacterium ulcerans Agy99 4550271 YP_906307.1 CDS MUL_2490 NC_008611.1 2782433 2782810 R cytoplasmic protein; hypothetical protein complement(2782433..2782810) Mycobacterium ulcerans Agy99 4550272 YP_906308.1 CDS MUL_2491 NC_008611.1 2782951 2783592 R cytoplasmic protein; hypothetical protein complement(2782951..2783592) Mycobacterium ulcerans Agy99 4550273 YP_906309.1 CDS MUL_5082 NC_008611.1 2783690 2783923 R cytoplasmic protein; hypothetical protein complement(2783690..2783923) Mycobacterium ulcerans Agy99 4550274 YP_906310.1 CDS MUL_2493 NC_008611.1 2784053 2784571 R cytoplasmic protein; hypothetical protein complement(2784053..2784571) Mycobacterium ulcerans Agy99 4550275 YP_906311.1 CDS gltA1 NC_008611.1 2784932 2786065 R catalyzes the formation of citrate from acetyl-CoA and oxaloacetate; citrate synthase complement(2784932..2786065) Mycobacterium ulcerans Agy99 4550276 YP_906312.1 CDS MUL_2496 NC_008611.1 2786978 2788567 R Detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; hypothetical protein complement(2786978..2788567) Mycobacterium ulcerans Agy99 4550278 YP_906313.1 CDS MUL_2497 NC_008611.1 2788573 2790024 D cytoplasmic protein; involved in transcriptional mechanism; transcriptional regulatory protein 2788573..2790024 Mycobacterium ulcerans Agy99 4550243 YP_906314.1 CDS MUL_2498 NC_008611.1 2790075 2791010 R cytoplasmic protein; hypothetical protein complement(2790075..2791010) Mycobacterium ulcerans Agy99 4550244 YP_906315.1 CDS MUL_2499 NC_008611.1 2791007 2791561 R cytoplasmic protein; hypothetical protein complement(2791007..2791561) Mycobacterium ulcerans Agy99 4550245 YP_906316.1 CDS atsD_1 NC_008611.1 2791573 2793708 R cytoplasmic protein; thought to play an important role in the mineralization of sulfates [catalytic activity: a phenol sulfate + H2O = a phenol + sulfate]; arylsulfatase AtsD complement(2791573..2793708) Mycobacterium ulcerans Agy99 4550246 YP_906317.1 CDS MUL_2502 NC_008611.1 2794174 2794548 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown, possible role in posttranslational modification, protein turnover, chaperonefunctions; FK-506 binding protein, peptidyl-prolyl cis- trans isomerase complement(2794174..2794548) Mycobacterium ulcerans Agy99 4550247 YP_906318.1 CDS MUL_2504 NC_008611.1 2795113 2796126 D cytoplasmic protein; hypothetical protein 2795113..2796126 Mycobacterium ulcerans Agy99 4550249 YP_906319.1 CDS MUL_2505 NC_008611.1 2796271 2799258 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(2796271..2799258) Mycobacterium ulcerans Agy99 4550250 YP_906320.1 CDS MUL_2506 NC_008611.1 2799380 2800402 R secreted protein; serine protease complement(2799380..2800402) Mycobacterium ulcerans Agy99 4550251 YP_906321.1 CDS MUL_2507 NC_008611.1 2800481 2801899 D cytoplasmic protein; hypothetical protein 2800481..2801899 Mycobacterium ulcerans Agy99 4550252 YP_906322.1 CDS MUL_2508 NC_008611.1 2801938 2802777 D cytoplasmic protein; hypothetical protein 2801938..2802777 Mycobacterium ulcerans Agy99 4550253 YP_906323.1 CDS MUL_2509 NC_008611.1 2802797 2803015 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(2802797..2803015) Mycobacterium ulcerans Agy99 4550254 YP_906324.1 CDS MUL_2510 NC_008611.1 2803162 2804505 D thought to be involved in active transport across the membrane. responsible for energy coupling to the transport system.; ABC transporter ATP-binding protein 2803162..2804505 Mycobacterium ulcerans Agy99 4550255 YP_906325.1 CDS whiB7 NC_008611.1 2804608 2804871 R cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein Whib-like WhiB7 complement(2804608..2804871) Mycobacterium ulcerans Agy99 4550256 YP_906326.1 CDS uvrD2 NC_008611.1 2805302 2807401 R cytoplasmic protein; involved in nucleotide excision repair. has both ATPase and helicase activities. unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single- stranded region is present. involved in the postincision events of nucleotide excision repair and methyl-directed mismatch repair.; ATP-dependent DNA helicase II UvrD2 complement(2805302..2807401) Mycobacterium ulcerans Agy99 4550257 YP_906327.1 CDS MUL_2513 NC_008611.1 2807557 2807811 D cytoplasmic protein; glutaredoxin protein 2807557..2807811 Mycobacterium ulcerans Agy99 4550258 YP_906328.1 CDS MUL_2514 NC_008611.1 2807777 2808823 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(2807777..2808823) Mycobacterium ulcerans Agy99 4550259 YP_906329.1 CDS MUL_2515 NC_008611.1 2809234 2810622 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 2809234..2810622 Mycobacterium ulcerans Agy99 4550155 YP_906330.1 CDS MUL_2516 NC_008611.1 2810693 2811757 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(2810693..2811757) Mycobacterium ulcerans Agy99 4550156 YP_906331.1 CDS lpqQ NC_008611.1 2812222 2813031 D membrane protein; lipoprotein LpqQ 2812222..2813031 Mycobacterium ulcerans Agy99 4550157 YP_906332.1 CDS nudC NC_008611.1 2813035 2813973 R can catalyze hydrolysis of broad range of dinucleotide pyrophosphates but prefers reduced form of NADH; requires divalent metal ions such as magnesium and manganese and produces two mononucleoside 5'-phosphates; NADH pyrophosphatase complement(2813035..2813973) Mycobacterium ulcerans Agy99 4550158 YP_906333.1 CDS MUL_2519 NC_008611.1 2813963 2815141 R membrane protein; thought to be involved in cation transport across the membrane.; transmembrane cation transporter complement(2813963..2815141) Mycobacterium ulcerans Agy99 4550159 YP_906334.1 CDS MUL_2520 NC_008611.1 2815203 2818508 R Detected in the membrane fraction by proteomics.; membrane protein; has both ATPase and helicase activities; ATP-dependent DNA helicase complement(2815203..2818508) Mycobacterium ulcerans Agy99 4550160 YP_906335.1 CDS MUL_2521 NC_008611.1 2818505 2821678 R membrane protein; has both ATPase and helicase activities; ATP-dependent DNA helicase complement(2818505..2821678) Mycobacterium ulcerans Agy99 4550161 YP_906336.1 CDS MUL_2522 NC_008611.1 2821824 2822666 R membrane protein; hypothetical protein complement(2821824..2822666) Mycobacterium ulcerans Agy99 4550162 YP_906337.1 CDS MUL_2523 NC_008611.1 2822812 2823057 D cytoplasmic protein; hypothetical protein 2822812..2823057 Mycobacterium ulcerans Agy99 4550163 YP_906338.1 CDS lipV NC_008611.1 2823122 2823907 D function unknown, presumed lipolytic enzyme involved in cellular metabolism.; lipase LipV 2823122..2823907 Mycobacterium ulcerans Agy99 4550164 YP_906339.1 CDS MUL_2525 NC_008611.1 2823910 2824218 D cytoplasmic protein; causes methylation of DNA.; DNA-methyltransferase (modification methylase) 2823910..2824218 Mycobacterium ulcerans Agy99 4550165 YP_906340.1 CDS MUL_2526 NC_008611.1 2824215 2825129 R cytoplasmic protein; hypothetical protein complement(2824215..2825129) Mycobacterium ulcerans Agy99 4550166 YP_906341.1 CDS moeB1 NC_008611.1 2825135 2826316 R The proteins in this cluster have high sequence similarity to MoeB and are possibly involved in the synthesis of molybdopterin, but there has been no biochemical or physiological characterization. There is also no genetic linkage to other molybdopterin cofactor synthesis proteins. These proteins are similar to a Pseudomonas stutzeri protein which is essential to pyridine-2,6-bis(thiocarboxylic acid) synthesis that possibly activates a substrate by adenylation; molybdopterin biosynthesis-like protein MoeZ complement(2825135..2826316) Mycobacterium ulcerans Agy99 4550167 YP_906342.1 CDS MUL_2528 NC_008611.1 2826488 2827471 R hypothetical protein complement(2826488..2827471) Mycobacterium ulcerans Agy99 4550168 YP_906343.1 CDS MUL_2529 NC_008611.1 2827699 2828382 D cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator 2827699..2828382 Mycobacterium ulcerans Agy99 4550169 YP_906344.1 CDS MUL_2530 NC_008611.1 2828379 2828624 R Detected in the cytoplamic fraction by LC-MS/MS. Also detected in the membrane fraction by proteomics (2D- LC-MS/MS); cytoplasmic protein; hypothetical protein complement(2828379..2828624) Mycobacterium ulcerans Agy99 4550170 YP_906345.1 CDS MUL_2531 NC_008611.1 2829066 2829620 D membrane protein; putative threonine and proline rich protein 2829066..2829620 Mycobacterium ulcerans Agy99 4550171 YP_906346.1 CDS MUL_2532 NC_008611.1 2829633 2830328 R cytoplasmic protein; hypothetical protein complement(2829633..2830328) Mycobacterium ulcerans Agy99 4550172 YP_906347.1 CDS rhlE NC_008611.1 2830591 2832144 D cytoplasmic protein; has a helix-destabilizing activity; ATP-dependent RNA helicase RhlE 2830591..2832144 Mycobacterium ulcerans Agy99 4550061 YP_906348.1 CDS MUL_2534 NC_008611.1 2832159 2833373 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 2832159..2833373 Mycobacterium ulcerans Agy99 4550062 YP_906349.1 CDS MUL_2535 NC_008611.1 2833396 2834196 R cytoplasmic protein; possibly involved in control of chromosome partitioning.; Soj/ParA-related protein complement(2833396..2834196) Mycobacterium ulcerans Agy99 4550063 YP_906350.1 CDS gpm2 NC_008611.1 2834198 2834872 D forms a homodimer in Mycobacterium tuberculosis; belongs to the dPGM superfamily; acid phosphatase 2834198..2834872 Mycobacterium ulcerans Agy99 4550064 YP_906351.1 CDS entC NC_008611.1 2834869 2835969 D cytoplasmic protein; could be involved in enterobactin biosynthesis. enterobactin is an iron-chelating compound involved in transporting iron from the bacterial environment into the cell cytoplasm. could be also involved in 2,3- dihydroxybenzoate or enterochelin or menaquinone biosynthesis [catalytic activity: chorismate = isochorismate]; isochorismate synthase EntC 2834869..2835969 Mycobacterium ulcerans Agy99 4550065 YP_906352.1 CDS MUL_2538 NC_008611.1 2835966 2836448 D cytoplasmic protein; function unknown, involved in cellular metabolism.; acetyltransferase 2835966..2836448 Mycobacterium ulcerans Agy99 4550066 YP_906353.1 CDS MUL_2539 NC_008611.1 2836400 2836831 R membrane protein; hypothetical protein complement(2836400..2836831) Mycobacterium ulcerans Agy99 4550067 YP_906354.1 CDS MUL_2540 NC_008611.1 2836956 2837921 D cytoplasmic protein; hypothetical protein 2836956..2837921 Mycobacterium ulcerans Agy99 4550068 YP_906355.1 CDS whiB1 NC_008611.1 2838205 2838459 D cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein Whib-like WhiB1 2838205..2838459 Mycobacterium ulcerans Agy99 4550069 YP_906356.1 CDS MUL_2542 NC_008611.1 2838522 2840021 R membrane protein; sensor part of a two component regulatory system.; sensor kinase from two component regulatory system complement(2838522..2840021) Mycobacterium ulcerans Agy99 4550070 YP_906357.1 CDS MUL_2543 NC_008611.1 2840071 2840535 R cytoplasmic protein; hypothetical protein complement(2840071..2840535) Mycobacterium ulcerans Agy99 4550071 YP_906358.1 CDS MUL_2544 NC_008611.1 2840648 2840953 R cytoplasmic protein; binds sigma factor and inhibits it. probably involved in survival following heat shock and oxidative stress.; anti-sigma factor complement(2840648..2840953) Mycobacterium ulcerans Agy99 4550072 YP_906359.1 CDS sigH NC_008611.1 2840950 2841600 R Member of the extracytoplasmic function sigma factors which are active under specific conditions; binds with the catalytic core of RNA polymerase to produce the holoenzyme and directs bacterial core RNA polymerase to specific promoter elements to initiate transcription; this sigma factor is involved in heat shock and oxidative stress response; RNA polymerase sigma factor RpoE complement(2840950..2841600) Mycobacterium ulcerans Agy99 4550073 YP_906360.1 CDS MUL_2546 NC_008611.1 2841917 2842765 D Detected in the cytoplamic fraction by LC-MS/MS. Also detected in the membrane fraction by proteomics (2D- LC-MS/MS); cytoplasmic protein; function unknown, probably involved in cellular metabolism.; short chain dehydrogenase 2841917..2842765 Mycobacterium ulcerans Agy99 4550074 YP_906361.1 CDS MUL_2547 NC_008611.1 2842901 2843236 D membrane protein; hypothetical protein 2842901..2843236 Mycobacterium ulcerans Agy99 4550075 YP_906362.1 CDS MUL_2549 NC_008611.1 2845567 2845914 R cytoplasmic protein; hypothetical protein complement(2845567..2845914) Mycobacterium ulcerans Agy99 4550077 YP_906363.1 CDS MUL_2550 NC_008611.1 2845911 2847431 R cytoplasmic protein; hypothetical protein complement(2845911..2847431) Mycobacterium ulcerans Agy99 4550078 YP_906364.1 CDS MUL_2551 NC_008611.1 2847510 2848106 R cytoplasmic protein; hypothetical protein complement(2847510..2848106) Mycobacterium ulcerans Agy99 4550079 YP_906365.1 CDS MUL_2552 NC_008611.1 2848329 2848718 R cytoplasmic protein; hypothetical protein complement(2848329..2848718) Mycobacterium ulcerans Agy99 4550080 YP_906366.1 CDS MUL_2553 NC_008611.1 2849026 2849340 R hypothetical protein complement(2849026..2849340) Mycobacterium ulcerans Agy99 4550042 YP_906367.1 CDS MUL_2554 NC_008611.1 2849416 2850714 R membrane protein; hypothetical protein complement(2849416..2850714) Mycobacterium ulcerans Agy99 4550043 YP_906368.1 CDS MUL_2555 NC_008611.1 2851502 2853409 R membrane protein; may be involved in regulation; hypothetical protein complement(2851502..2853409) Mycobacterium ulcerans Agy99 4550044 YP_906369.1 CDS MUL_2556 NC_008611.1 2853419 2853742 R cytoplasmic protein; hypothetical protein complement(2853419..2853742) Mycobacterium ulcerans Agy99 4550045 YP_906370.1 CDS MUL_2557 NC_008611.1 2853847 2855064 R hypothetical protein complement(2853847..2855064) Mycobacterium ulcerans Agy99 4550046 YP_906371.1 CDS fadB3 NC_008611.1 2855189 2856073 R cytoplasmic protein; thought to be involved in fatty acid degradation. FadB and FadA are the alpha and BetA subunits of the multifunctional enzyme complex of the fatty acid degradation cycle [catalytic activity: (S)-3- hydroxybutanoyl-CoA + NADP+ = 3-acetoacetyl-CoA + NADPH]; 3-hydroxybutyryl-CoA dehydrogenase FadB3 complement(2855189..2856073) Mycobacterium ulcerans Agy99 4550047 YP_906372.1 CDS MUL_2559 NC_008611.1 2856171 2856944 R cytoplasmic protein; hypothetical protein complement(2856171..2856944) Mycobacterium ulcerans Agy99 4550048 YP_906373.1 CDS aroA NC_008611.1 2856999 2858294 D catalyzes the formation of 5-O-(1-carboxyvinyl)-3-phosphoshikimate from phosphoenolpyruvate and 3-phosphoshikimate in tryptophan biosynthesis; 3-phosphoshikimate 1-carboxyvinyltransferase 2856999..2858294 Mycobacterium ulcerans Agy99 4550049 YP_906374.1 CDS MUL_2561 NC_008611.1 2858291 2859283 D cytoplasmic protein; hypothetical protein 2858291..2859283 Mycobacterium ulcerans Agy99 4550050 YP_906375.1 CDS MUL_2562 NC_008611.1 2859270 2859737 R cytoplasmic protein; function unknown. could be involved in regulatory mechanism.; transcriptional regulatory protein complement(2859270..2859737) Mycobacterium ulcerans Agy99 4550051 YP_906376.1 CDS fadA6_4 NC_008611.1 2859832 2861013 D cytoplasmic protein; function unknown, but involved in lipid metabolism [catalytic activity: 2 acetyl-CoA = CoA + acetoacetyl- CoA]; acetyl-CoA acetyltransferase FadA6_4 2859832..2861013 Mycobacterium ulcerans Agy99 4550052 YP_906377.1 CDS desA3_1 NC_008611.1 2861051 2862337 R cytoplasmic protein; thought to be involved in lipid metabolism [catalytic activity: linoleoyl-CoA + ah(2) + O(2) = gamma- linolenoyl-CoA + a + 2 H(2)O]; linoleoyl-CoA desaturase, DesA3 complement(2861051..2862337) Mycobacterium ulcerans Agy99 4550053 YP_906378.1 CDS desA3 NC_008611.1 2862565 2863851 R cytoplasmic protein; thought to be involved in lipid metabolism [catalytic activity: linoleoyl-CoA + ah(2) + O(2) = gamma- linolenoyl-CoA + a + 2 H(2)O]; linoleoyl-CoA desaturase, DesA3 complement(2862565..2863851) Mycobacterium ulcerans Agy99 4550054 YP_906379.1 CDS MUL_2566 NC_008611.1 2863940 2865094 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase complement(2863940..2865094) Mycobacterium ulcerans Agy99 4550055 YP_906380.1 CDS MUL_2567 NC_008611.1 2865235 2865735 R cytoplasmic protein; hypothetical protein complement(2865235..2865735) Mycobacterium ulcerans Agy99 4550056 YP_906381.1 CDS pvdS NC_008611.1 2865801 2866673 R cytoplasmic protein; possibly involved in transcriptional mechanism (probably sigma factor promoting attachment of the RNA polymerase to specific initiation sites); transcriptional regulatory protein PvdS complement(2865801..2866673) Mycobacterium ulcerans Agy99 4550057 YP_906382.1 CDS MUL_2572 NC_008611.1 2868896 2870041 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; probably involved in transport of undeterminated substrate (possibly cations Na/H) across the membrane. thought to be responsible for the translocation of the substrate across the membrane.; membrane transporter complement(2868896..2870041) Mycobacterium ulcerans Agy99 4550060 YP_906383.1 CDS MUL_2573 NC_008611.1 2870046 2870528 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown but has a putative regulatory, ligand-binding protein related to C-terminal domains of K+ channels [inorganic ion transport and metabolism]; hypothetical protein complement(2870046..2870528) Mycobacterium ulcerans Agy99 4550026 YP_906384.1 CDS MUL_2574 NC_008611.1 2870657 2871403 R membrane protein; hypothetical protein complement(2870657..2871403) Mycobacterium ulcerans Agy99 4550027 YP_906385.1 CDS secA1 NC_008611.1 2874774 2877626 R functions in protein export; can interact with acidic membrane phospholipids and the SecYEG protein complex; binds to preproteins; binds to ATP and undergoes a conformational change to promote membrane insertion of SecA/bound preprotein; ATP hydrolysis appears to drive release of the preprotein from SecA and deinsertion of SecA from the membrane; additional proteins SecD/F/YajC aid SecA recycling; exists in an equilibrium between monomers and dimers; may possibly form higher order oligomers; proteins in this cluster correspond SecA1; SecA2 is not essential and seems to play a role in secretion of a subset of proteins; preprotein translocase subunit SecA complement(2874774..2877626) Mycobacterium ulcerans Agy99 4550029 YP_906386.1 CDS MUL_2577 NC_008611.1 2877723 2878412 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown but has domain identity with sigma 54 modulation protein; hypothetical protein complement(2877723..2878412) Mycobacterium ulcerans Agy99 4550030 YP_906387.1 CDS MUL_2578 NC_008611.1 2878759 2879394 R cytoplasmic protein; function unknown, but contains amidophosphoribosyltransferase domain; hypothetical protein complement(2878759..2879394) Mycobacterium ulcerans Agy99 4550031 YP_906388.1 CDS MUL_2579 NC_008611.1 2879437 2880267 R membrane protein; hypothetical protein complement(2879437..2880267) Mycobacterium ulcerans Agy99 4550032 YP_906389.1 CDS lpqB NC_008611.1 2880328 2882088 R membrane protein; lipoprotein LpqB complement(2880328..2882088) Mycobacterium ulcerans Agy99 4550033 YP_906390.1 CDS mtrB NC_008611.1 2882106 2883785 R membrane protein; sensor part of a two component regulatory system.; two component sensory transduction histidine kinase MtrB complement(2882106..2883785) Mycobacterium ulcerans Agy99 4550034 YP_906391.1 CDS mtrA NC_008611.1 2883835 2884521 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics.; cytoplasmic protein; transcriptional activator part of a two component regulatory system.; two component sensory transduction transcriptional regulatory protein MtrA complement(2883835..2884521) Mycobacterium ulcerans Agy99 4550035 YP_906392.1 CDS tmk NC_008611.1 2884576 2885244 R catalyzes the reversible phosphoryl transfer from adenosine triphosphate (ATP) to thymidine monophosphate (dTMP) to form thymidine diphosphate (dTDP); thymidylate kinase complement(2884576..2885244) Mycobacterium ulcerans Agy99 4550036 YP_906393.1 CDS MUL_2585 NC_008611.1 2886621 2887667 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 2886621..2887667 Mycobacterium ulcerans Agy99 4550038 YP_906394.1 CDS MUL_2587 NC_008611.1 2889049 2890095 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 2889049..2890095 Mycobacterium ulcerans Agy99 4550039 YP_906395.1 CDS sahH NC_008611.1 2892768 2894246 R catalyzes the formation of L-homocysteine from S-adenosyl-L-homocysteine; S-adenosyl-L-homocysteine hydrolase complement(2892768..2894246) Mycobacterium ulcerans Agy99 4549868 YP_906396.1 CDS MUL_2593 NC_008611.1 2894351 2895058 R cytoplasmic protein; probably involved in a transcriptional mechanism; TetR family transcriptional regulator complement(2894351..2895058) Mycobacterium ulcerans Agy99 4549869 YP_906397.1 CDS rubB NC_008611.1 2894998 2895180 R cytoplasmic protein; involved in the hydrocarbon hydroxylating system to convert conversion of dodecane to lauric acid, which transfers electrons from NADH to rubredoxin reductase and then through rubredoxin to alkane 1 monooxygenase.; rubredoxin RubB complement(2894998..2895180) Mycobacterium ulcerans Agy99 4549870 YP_906398.1 CDS rubA NC_008611.1 2895181 2895351 R cytoplasmic protein; involved in the hydrocarbon hydroxylating system, which transfers electrons from NADH to rubredoxin reductase and then through rubredoxin to alkane 1 monooxygenase.; rubredoxin RubA complement(2895181..2895351) Mycobacterium ulcerans Agy99 4549871 YP_906399.1 CDS MUL_2597 NC_008611.1 2896591 2898075 R membrane protein; thought to be involved in cationic amino acid transport across the membrane. responsible for the translocation of the substrate across the membrane.; cationic amino acid transport integral membrane protein complement(2896591..2898075) Mycobacterium ulcerans Agy99 4549873 YP_906400.1 CDS MUL_2598 NC_008611.1 2898132 2899526 D hypothetical protein 2898132..2899526 Mycobacterium ulcerans Agy99 4549874 YP_906401.1 CDS MUL_2599 NC_008611.1 2899569 2900975 D cytoplasmic protein; function unknown but contains dehydrogenase domain; hypothetical protein 2899569..2900975 Mycobacterium ulcerans Agy99 4549875 YP_906402.1 CDS manA NC_008611.1 2900953 2902179 R cytoplasmic protein; this enzyme converts D-mannose 6-phosphate to D- fructose 6-phosphate [catalytic activity: D-mannose 6- phosphate = D-fructose 6-phosphate]; mannose-6-phosphate isomerase ManA complement(2900953..2902179) Mycobacterium ulcerans Agy99 4549876 YP_906403.1 CDS MUL_2601 NC_008611.1 2902187 2903296 R cytoplasmic protein; hypothetical protein complement(2902187..2903296) Mycobacterium ulcerans Agy99 4549877 YP_906404.1 CDS manB NC_008611.1 2903293 2904693 R converts mannose-6-phosphate to mannose-1-phosphate; the resulting product is then converted to GDP-mannose by ManC which is then used in the synthesis of mannose-containing glycoconjugates that are important for mediating entry into host cells; phosphomannomutase complement(2903293..2904693) Mycobacterium ulcerans Agy99 4549878 YP_906405.1 CDS MUL_2603 NC_008611.1 2904840 2905337 R cytoplasmic protein; hypothetical protein complement(2904840..2905337) Mycobacterium ulcerans Agy99 4549879 YP_906406.1 CDS MUL_2604 NC_008611.1 2905352 2905882 D cytoplasmic protein; hypothetical protein 2905352..2905882 Mycobacterium ulcerans Agy99 4549880 YP_906407.1 CDS whiB2 NC_008611.1 2905948 2906331 R cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein Whib-like WhiB2 complement(2905948..2906331) Mycobacterium ulcerans Agy99 4549881 YP_906408.1 CDS fbiA NC_008611.1 2906724 2907713 D catalyzes the formation of the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) and GMP from actyl (2) diphospho-(5')guanosine (LPPG) to 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO); LPPG:FO 2-phospho-L-lactate transferase 2906724..2907713 Mycobacterium ulcerans Agy99 4549882 YP_906409.1 CDS fbiB NC_008611.1 2907710 2909074 D catalyzes the addition of gamma linked glutamate to 7,8-didemethyl-8-hydroxy-5-deazariboflavin coenzyme F420-0); F420-0--gamma-glutamyl ligase 2907710..2909074 Mycobacterium ulcerans Agy99 4549883 YP_906410.1 CDS MUL_2608 NC_008611.1 2909103 2910689 D cytoplasmic protein; causes DNA methylation.; DNA methylase 2909103..2910689 Mycobacterium ulcerans Agy99 4549884 YP_906411.1 CDS MUL_2609 NC_008611.1 2910728 2911234 D cytoplasmic protein; hypothetical protein 2910728..2911234 Mycobacterium ulcerans Agy99 4549885 YP_906412.1 CDS manB NC_008611.1 2911263 2912339 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; involved in GDP-mannose biosynthesis and biosynthesis of nucleotide-activated glycero-manno-heptose (D-alpha-D pathway): generates GDP-mannose and phosphate from GTP and alpha-D-mannose 1-phosphate. ManB product is needed for all mannosyl glycolipids and polysaccharides which, like rhamnosyl residues, are an important part of the mycobacterium envelope [catalytic activity: alpha-D- mannose 1-phosphate + GTP = GDP-mannose + phosphate]; d-alpha-D-mannose-1-phosphate guanylyltransferase ManB complement(2911263..2912339) Mycobacterium ulcerans Agy99 4549886 YP_906413.1 CDS wbbL1 NC_008611.1 2912341 2913225 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in cell wall arabinogalactan linker formation: uses dTDP-L-rhamnose as substrate to insert the rhamnosyl residue into the cell wall. seems to be essential for mycobacterial viability.; dTDP-rha:A-D-GlcNAc-diphosphoryl polyprenol A-3- L-rhamnosyl transferase, WbbL1 complement(2912341..2913225) Mycobacterium ulcerans Agy99 4549849 YP_906414.1 CDS rmlD NC_008611.1 2913218 2914144 R cytoplasmic protein; involved in dTDP-L-rhamnose biosynthesis: converts dTDP-6-deoxy-L-lyxo-4-hexulose to dTDP-L-rhamnose with the concomitant oxidation of NADPH to NADP+ [catalytic activity: dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH = dTDP-L- rhamnose + NADP+]; dTDP-6-deoxy-L-lyxo-4-hexulose reductase RmlD complement(2913218..2914144) Mycobacterium ulcerans Agy99 4549850 YP_906415.1 CDS MUL_2613 NC_008611.1 2914209 2915669 D membrane protein; hypothetical protein 2914209..2915669 Mycobacterium ulcerans Agy99 4549851 YP_906416.1 CDS MUL_2614 NC_008611.1 2915699 2916379 D hypothetical protein 2915699..2916379 Mycobacterium ulcerans Agy99 4549852 YP_906417.1 CDS MUL_2615 NC_008611.1 2916495 2916773 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown.; hypothetical protein 2916495..2916773 Mycobacterium ulcerans Agy99 4549853 YP_906418.1 CDS ctpC NC_008611.1 2916783 2918981 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; metal cation-transporting ATPase; possibly catalyzes the transport of undetermined metal cation with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + undeterminated metal cation(in) = ADP + phosphate + undeterminated metal cation(out)]; metal cation-transporting p-type ATPase CtpC 2916783..2918981 Mycobacterium ulcerans Agy99 4549854 YP_906419.1 CDS MUL_2617 NC_008611.1 2919025 2919726 R cytoplasmic protein; hypothetical protein complement(2919025..2919726) Mycobacterium ulcerans Agy99 4549855 YP_906420.1 CDS MUL_2620 NC_008611.1 2921595 2922341 D cytoplasmic protein; function unknown, potential regulatory function; hypothetical protein 2921595..2922341 Mycobacterium ulcerans Agy99 4549857 YP_906421.1 CDS MUL_2621 NC_008611.1 2922436 2923623 D cytoplasmic protein; acyl-CoA transferase 2922436..2923623 Mycobacterium ulcerans Agy99 4549858 YP_906422.1 CDS MUL_2623 NC_008611.1 2926160 2926600 R membrane protein; sensor part of the two component regulatory system.; two component transcriptional regulator complement(2926160..2926600) Mycobacterium ulcerans Agy99 4549860 YP_906423.1 CDS MUL_2624 NC_008611.1 2926597 2928219 R membrane protein; two component system membrane associated sensor kinase complement(2926597..2928219) Mycobacterium ulcerans Agy99 4549861 YP_906424.1 CDS MUL_2625 NC_008611.1 2928216 2929454 R cytoplasmic protein; hypothetical protein complement(2928216..2929454) Mycobacterium ulcerans Agy99 4549862 YP_906425.1 CDS fadE25 NC_008611.1 2929648 2930817 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown, but involved in lipid metabolism [catalytic activity: acyl-CoA + ETF = 2,3-dehydroacyl-CoA + reduced ETF]; acyl-CoA dehydrogenase FadE25 complement(2929648..2930817) Mycobacterium ulcerans Agy99 4549863 YP_906426.1 CDS purE NC_008611.1 2930842 2931357 R Detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; nvolved in purine biosynthesis (sixth step) this subunit can alone transform air to cair, but in association with PurK, which possesses an ATPase activity, an enzyme complex is produced which is capable of converting air to cair efficiently under physiol; phosphoribosylaminoimidazole carboxylase catalytic subunit PurE complement(2930842..2931357) Mycobacterium ulcerans Agy99 4549864 YP_906427.1 CDS purK NC_008611.1 2931350 2932624 R With PurE catalyzes the conversion of aminoimidazole ribonucleotide to carboxyaminoimidazole ribonucleotide in the de novo purine nucleotide biosynthetic pathway; phosphoribosylaminoimidazole carboxylase ATPase subunit complement(2931350..2932624) Mycobacterium ulcerans Agy99 4549865 YP_906428.1 CDS MUL_2629 NC_008611.1 2932664 2933347 D membrane protein; hypothetical protein 2932664..2933347 Mycobacterium ulcerans Agy99 4549866 YP_906429.1 CDS MUL_2630 NC_008611.1 2933302 2933820 R membrane protein; hypothetical protein complement(2933302..2933820) Mycobacterium ulcerans Agy99 4549867 YP_906430.1 CDS birA NC_008611.1 2933883 2934713 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl- CoA:carbon-dioxide ligase. this protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [catalytic activity: ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP forming)] = AMP + pyrophosphate + [acetyl- CoA:carbon-dioxide ligase (ADP forming)]]; bifunctional protein BirA complement(2933883..2934713) Mycobacterium ulcerans Agy99 4549829 YP_906431.1 CDS accD5 NC_008611.1 2934864 2936504 D Detected in the cytoplamic fraction by LC-MS/MS. Also detected in the extracellular matrix and membrane fraction by proteomics.; cytoplasmic protein; key enzyme in the catabolic pathway of odd-chain fatty acids, isoleucine, threonine, methionine, and valine [catalytic activity: ATP + propionyl-CoA + CO(2) + H(2)O = ADP + orthophosphate + methylmalonyl-CoA]; propionyl-CoA carboxylase beta chain 5 AccD5 2934864..2936504 Mycobacterium ulcerans Agy99 4549830 YP_906432.1 CDS MUL_2633 NC_008611.1 2936501 2936821 D cytoplasmic protein; hypothetical protein 2936501..2936821 Mycobacterium ulcerans Agy99 4549831 YP_906433.1 CDS MUL_2634 NC_008611.1 2936818 2937468 D Maf; overexpression in Bacillus subtilis inhibits septation in the dividing cell; Maf-like protein 2936818..2937468 Mycobacterium ulcerans Agy99 4549832 YP_906434.1 CDS sseA NC_008611.1 2937508 2938413 D cytoplasmic protein; possibly a sulfotransferase involved in the formation of thiosulfate [catalytic activity: thiosulfate + cyanide = sulfite + thiocyanate]; thiosulfate sulfurtransferase SseA 2937508..2938413 Mycobacterium ulcerans Agy99 4549833 YP_906435.1 CDS sufE NC_008611.1 2938410 2938829 D cytoplasmic protein; probably involved in Fe-S center assembly; Fe-S metabolism associated protein, SufE 2938410..2938829 Mycobacterium ulcerans Agy99 4549834 YP_906436.1 CDS accA3 NC_008611.1 2938940 2940733 D Detected in the cytoplasmic fraction by proteomics (2D-LC-MS/MS) Also detected in the extracellular matrix and membrane fraction by proteomics.; cytoplasmic protein; involved in long-chain fatty acid synthesis (at the first step) carries two functions: biotin carboxyl carrier protein and biotin carboxyltransferase [catalytic activity: ATP + biotin-carboxyl-carrier protein + CO(2) = ADP + orthophosphate + carboxybiotin-carboxyl-carrier protein]; bifunctional protein acetyl-/propionyl-coenzyme a carboxylase (alpha chain) AccA3 2938940..2940733 Mycobacterium ulcerans Agy99 4549835 YP_906437.1 CDS MUL_2638 NC_008611.1 2940775 2950209 R cytoplasmic protein; function unknown but contains 3 modules for the potential synthesis of a tripeptide; peptide synthetase Nrp (peptide synthase) complement(2940775..2950209) Mycobacterium ulcerans Agy99 4549836 YP_906438.1 CDS MUL_2639 NC_008611.1 2950837 2951406 D cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein 2950837..2951406 Mycobacterium ulcerans Agy99 4549837 YP_906439.1 CDS sigF NC_008611.1 2951468 2952256 R Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; this sigma factor is a general stress response regulator; expressed in stationary phase and under nitrogen depletion and cold shock; RNA polymerase sigma factor SigF complement(2951468..2952256) Mycobacterium ulcerans Agy99 4549838 YP_906440.1 CDS rsbW NC_008611.1 2952253 2952690 R cytoplasmic protein; binds to sigma and blocks its ability to form an RNA polymerase holoenzyme. regulates negatively SigF and negatively regulated by RsfA and RsfB.; anti-sigma factor RsbW complement(2952253..2952690) Mycobacterium ulcerans Agy99 4549839 YP_906441.1 CDS MUL_5106 NC_008611.1 2952768 2952980 R cytoplasmic protein; hypothetical protein complement(2952768..2952980) Mycobacterium ulcerans Agy99 4549840 YP_906442.1 CDS usfY NC_008611.1 2953173 2953484 R cytoplasmic protein; hypothetical protein complement(2953173..2953484) Mycobacterium ulcerans Agy99 4549841 YP_906443.1 CDS MUL_2643 NC_008611.1 2953597 2953983 R membrane protein; hypothetical protein complement(2953597..2953983) Mycobacterium ulcerans Agy99 4549842 YP_906444.1 CDS lat NC_008611.1 2954063 2955403 R catalyzes the formation of 2-aminoadipate 6-semiladehyde and glutamate from lysine and 2-oxoglutarate; L-lysine aminotransferase complement(2954063..2955403) Mycobacterium ulcerans Agy99 4549843 YP_906445.1 CDS MUL_2645 NC_008611.1 2955457 2955909 R cytoplasmic protein; involved in transcriptional mechanism.; AsnC family transcriptional regulator complement(2955457..2955909) Mycobacterium ulcerans Agy99 4549844 YP_906446.1 CDS MUL_2646 NC_008611.1 2955943 2957214 D cytoplasmic protein; hypothetical protein 2955943..2957214 Mycobacterium ulcerans Agy99 4549845 YP_906447.1 CDS pcd NC_008611.1 2957292 2958806 D cytoplasmic protein; involved in L-alpha-aminoadipic acid (L-AAA) biosynthesis (in the second step; the first step is promoted by Lat enzyme.; piperideine-6-carboxilic acid dehydrogenase Pcd 2957292..2958806 Mycobacterium ulcerans Agy99 4549846 YP_906448.1 CDS MUL_2648 NC_008611.1 2958855 2959529 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator 2958855..2959529 Mycobacterium ulcerans Agy99 4549847 YP_906449.1 CDS nei NC_008611.1 2964187 2964951 D cytoplasmic protein; involved in damage reversal. DNA N-glycosylase with an ap lyase activity. required for the repair of oxidative DNA damage (oxidized pyrimidines); endonuclease VIII Nei 2964187..2964951 Mycobacterium ulcerans Agy99 4549775 YP_906450.1 CDS lpqC NC_008611.1 2964966 2965883 R membrane protein; function unknown, lipolytic enzyme involved in cellular metabolism.; esterase lipoprotein LpqC complement(2964966..2965883) Mycobacterium ulcerans Agy99 4549776 YP_906451.1 CDS MUL_2653 NC_008611.1 2966407 2966724 R cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 complement(2966407..2966724) Mycobacterium ulcerans Agy99 4549778 YP_906452.1 CDS MUL_2654 NC_008611.1 2966749 2967453 R cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 complement(2966749..2967453) Mycobacterium ulcerans Agy99 4549779 YP_906453.1 CDS phoS2_1 NC_008611.1 2968550 2969662 R membrane protein; involved in active transport of inorganic phosphate across the membrane (import) this is one of the proteins required for binding-protein-mediated phosphate transport.; periplasmic phosphate-binding lipoprotein PhoS2 complement(2968550..2969662) Mycobacterium ulcerans Agy99 4549780 YP_906454.1 CDS MUL_2657 NC_008611.1 2969740 2970243 D cytoplasmic protein; hypothetical protein 2969740..2970243 Mycobacterium ulcerans Agy99 4549781 YP_906455.1 CDS atsB NC_008611.1 2970265 2973156 R membrane protein; generates sulfate and phenol from phenol sulfate [catalytic activity: a phenol sulfate + H(2)O = a phenol + sulfate]; arylsulfatase AtsB complement(2970265..2973156) Mycobacterium ulcerans Agy99 4549782 YP_906456.1 CDS MUL_2659 NC_008611.1 2973178 2974044 R cytoplasmic protein; catalyses the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil, psi); pseudouridine synthase complement(2973178..2974044) Mycobacterium ulcerans Agy99 4549783 YP_906457.1 CDS phoY1 NC_008611.1 2975289 2975954 R cytoplasmic protein; involved in transcriptional regulation of active transport of inorganic phosphate across the membrane.; phosphate-transport system transcriptional regulatory protein PhoY1 complement(2975289..2975954) Mycobacterium ulcerans Agy99 4549785 YP_906458.1 CDS gltB_1 NC_008611.1 2981516 2983060 R membrane protein; function unknown but may be involved in glutamate biosynthesis [catalytic activity: 2 L-glutamate + NADP(+) = L-glutamine + 2-oxoglutarate + NADPH]; glutamate synthase, GltB complement(2981516..2983060) Mycobacterium ulcerans Agy99 4549789 YP_906459.1 CDS glpD2 NC_008611.1 2983468 2985225 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); cytoplasmic protein; involved in aerobic respiration and oxydation of glycerol. reduces an acceptor and generates glycerone phosphate from SN-glycerol 3-phosphate. possibly play a role in metabolism of riboflavin, fad,FMN [catalytic activity: SN-glycerol 3-phosphate + acceptor = glycerone phosphate + reduced acceptor]; glycerol-3-phosphate dehydrogenase GlpD2 complement(2983468..2985225) Mycobacterium ulcerans Agy99 4549790 YP_906460.1 CDS lpdA NC_008611.1 2985310 2986722 R catalyzes the reduction of nonspecific electron acceptors such as 2,6-dimethyl-1,4-benzoquinone and 5-hydroxy-1,4-naphthaquinone; does not have lipoamide dehydrogenase activity; flavoprotein disulfide reductase complement(2985310..2986722) Mycobacterium ulcerans Agy99 4549791 YP_906461.1 CDS MUL_2669 NC_008611.1 2986864 2987343 D cytoplasmic protein; hypothetical protein 2986864..2987343 Mycobacterium ulcerans Agy99 4549792 YP_906462.1 CDS MUL_2670 NC_008611.1 2987435 2988280 R cytoplasmic protein; hypothetical protein complement(2987435..2988280) Mycobacterium ulcerans Agy99 4549758 YP_906463.1 CDS amiA1 NC_008611.1 2988434 2989603 R membrane protein; function unknown, hydrolysis of L-amino acids.; N-acyl-L-amino acid amidohydrolase AmiA1 complement(2988434..2989603) Mycobacterium ulcerans Agy99 4549759 YP_906464.1 CDS amiB1 NC_008611.1 2989600 2990781 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; involved in cellular metabolism, active on carbon aliphatic amides and/or on many aromatic amides [catalytic activity : a monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)]; amidohydrolase complement(2989600..2990781) Mycobacterium ulcerans Agy99 4549760 YP_906465.1 CDS deoD NC_008611.1 2990864 2991685 D catalyzes the formation of a purine and ribose phosphate from a purine nucleoside; in E. coli this enzyme functions in xanthosine degradation; purine nucleoside phosphorylase 2990864..2991685 Mycobacterium ulcerans Agy99 4549761 YP_906466.1 CDS pmmB NC_008611.1 2991675 2993255 D cytoplasmic protein; converts D-mannose 1-phosphate to D-mannose 6- phosphate.; phosphomannomutase 2991675..2993255 Mycobacterium ulcerans Agy99 4549762 YP_906467.1 CDS upp NC_008611.1 2993257 2993826 R Catalyzes the formation of uracil and 5-phospho-alpha-D-ribosy 1-diphosphate from UMP and diphosphate; uracil phosphoribosyltransferase complement(2993257..2993826) Mycobacterium ulcerans Agy99 4549763 YP_906468.1 CDS MUL_2676 NC_008611.1 2994021 2994863 D cytoplasmic protein; involved in cellular metabolism: acting on ester bonds [catalytic activity: an orthophosphoric monoester + H(2)O = an alcohol + orthophosphate]; acid phosphatase 2994021..2994863 Mycobacterium ulcerans Agy99 4549764 YP_906469.1 CDS MUL_2677 NC_008611.1 2994912 2996237 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein 2994912..2996237 Mycobacterium ulcerans Agy99 4549765 YP_906470.1 CDS MUL_2678 NC_008611.1 2996294 2997190 R cytoplasmic protein; function unknown but contains hydrolase domain; hypothetical protein complement(2996294..2997190) Mycobacterium ulcerans Agy99 4549766 YP_906471.1 CDS MUL_2679 NC_008611.1 2997293 2998672 D cytoplasmic protein; function unknown. thought to be regulated by LexA; 13e12 repeat-containing protein 2997293..2998672 Mycobacterium ulcerans Agy99 4549767 YP_906472.1 CDS MUL_2680 NC_008611.1 2998872 3000269 D membrane protein; function unknown but contains permease domain and may be involved in nucleotide transport (cytosine/purines) and metabolism/coenzyme metabolism; hypothetical protein 2998872..3000269 Mycobacterium ulcerans Agy99 4549768 YP_906473.1 CDS MUL_2681 NC_008611.1 3000377 3001288 R Detected in the cytoplamic fraction by LCMSMS; cytoplasmic protein; function unknown, contains xylanase/chitin deacetylase domain; chitinase complement(3000377..3001288) Mycobacterium ulcerans Agy99 4549769 YP_906474.1 CDS alc NC_008611.1 3001285 3002244 R catalyzes the formation of S-ureidoglycolate and urea from allantoate; allantoicase complement(3001285..3002244) Mycobacterium ulcerans Agy99 4549770 YP_906475.1 CDS MUL_2683 NC_008611.1 3002241 3003539 R cytoplasmic protein; hypothetical protein complement(3002241..3003539) Mycobacterium ulcerans Agy99 4549771 YP_906476.1 CDS MUL_2685 NC_008611.1 3005342 3006388 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3005342..3006388 Mycobacterium ulcerans Agy99 4549773 YP_906477.1 CDS MUL_2686 NC_008611.1 3006538 3007185 R membrane protein; PPE family protein complement(3006538..3007185) Mycobacterium ulcerans Agy99 4549774 YP_906478.1 CDS MUL_2688 NC_008611.1 3008107 3008811 D cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 3008107..3008811 Mycobacterium ulcerans Agy99 4549689 YP_906479.1 CDS MUL_2689 NC_008611.1 3008836 3009153 D cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 3008836..3009153 Mycobacterium ulcerans Agy99 4549690 YP_906480.1 CDS MUL_2691 NC_008611.1 3010350 3010589 R cytoplasmic protein; transposition of an insertion sequence; transposase ISMmr01_aa2-like complement(3010350..3010589) Mycobacterium ulcerans Agy99 4549691 YP_906481.1 CDS MUL_2692 NC_008611.1 3010597 3010878 R cytoplasmic protein; transposition of an insertion sequence; transposase ISMmr01_aa1-like complement(3010597..3010878) Mycobacterium ulcerans Agy99 4549692 YP_906482.1 CDS MUL_2693 NC_008611.1 3011055 3012377 R cytoplasmic protein; hypothetical protein complement(3011055..3012377) Mycobacterium ulcerans Agy99 4549693 YP_906483.1 CDS pitB NC_008611.1 3012841 3014460 D membrane protein; involved in phosphate transport.; phosphate-transport permease PitB 3012841..3014460 Mycobacterium ulcerans Agy99 4549694 YP_906484.1 CDS cysH_1 NC_008611.1 3016215 3016949 D catalyzes the reduction of 3'-phosphoadenylyl sulfate into sulfite; phosphoadenosine phosphosulfate reductase 3016215..3016949 Mycobacterium ulcerans Agy99 4549696 YP_906485.1 CDS MUL_2697 NC_008611.1 3016946 3017665 D cytoplasmic protein; hypothetical protein 3016946..3017665 Mycobacterium ulcerans Agy99 4549697 YP_906486.1 CDS MUL_2698 NC_008611.1 3017686 3018624 R cytoplasmic protein; LysR family transcriptional regulator complement(3017686..3018624) Mycobacterium ulcerans Agy99 4549698 YP_906487.1 CDS fprA NC_008611.1 3018711 3019973 D secreted protein; generates oxidized ferredoxin from ferredoxin [catalytic activity: reduced ferredoxin + NADP(+) = oxidized ferredoxin + NADPH]; NADPH:adrenodoxin oxidoreductase FprA 3018711..3019973 Mycobacterium ulcerans Agy99 4549699 YP_906488.1 CDS fdxC_1 NC_008611.1 3020098 3020424 D cytoplasmic protein; ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.; ferredoxin FdxC 3020098..3020424 Mycobacterium ulcerans Agy99 4549700 YP_906489.1 CDS MUL_2701 NC_008611.1 3020551 3021144 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); cytoplasmic protein; function unknown. possibly involved in transcriptional mechanism.; transcriptional regulatory protein complement(3020551..3021144) Mycobacterium ulcerans Agy99 4549701 YP_906490.1 CDS MUL_2702 NC_008611.1 3021329 3021988 R membrane protein; PE-PGRS family protein complement(3021329..3021988) Mycobacterium ulcerans Agy99 4549702 YP_906491.1 CDS MUL_2703 NC_008611.1 3022424 3022798 R cytoplasmic protein; function unknown but contains zinc binding domain found at the N-terminus of the ClpX protein. ClpX is an ATPase which functions both as a substrate specificity component of the ClpXP protease and as a molecular chaperone.; hypothetical protein complement(3022424..3022798) Mycobacterium ulcerans Agy99 4549703 YP_906492.1 CDS lipM NC_008611.1 3022900 3024162 D membrane protein; hydrolysis of lipids (bound ester); membrane-bound esterase LipM 3022900..3024162 Mycobacterium ulcerans Agy99 4549704 YP_906493.1 CDS MUL_2705 NC_008611.1 3024174 3025160 D cytoplasmic protein; hypothetical protein 3024174..3025160 Mycobacterium ulcerans Agy99 4549705 YP_906494.1 CDS MUL_2706 NC_008611.1 3025349 3026689 D hypothetical protein 3025349..3026689 Mycobacterium ulcerans Agy99 4549669 YP_906495.1 CDS MUL_2707 NC_008611.1 3026722 3027918 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 3026722..3027918 Mycobacterium ulcerans Agy99 4549670 YP_906496.1 CDS MUL_2708 NC_008611.1 3027924 3028619 R cytoplasmic protein; hypothetical protein complement(3027924..3028619) Mycobacterium ulcerans Agy99 4549671 YP_906497.1 CDS MUL_2710 NC_008611.1 3028761 3029201 R membrane protein; hypothetical protein complement(3028761..3029201) Mycobacterium ulcerans Agy99 4549672 YP_906498.1 CDS MUL_2713 NC_008611.1 3031647 3032693 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3031647..3032693 Mycobacterium ulcerans Agy99 4549675 YP_906499.1 CDS MUL_2714 NC_008611.1 3033892 3034542 D cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator 3033892..3034542 Mycobacterium ulcerans Agy99 4549676 YP_906500.1 CDS MUL_2716 NC_008611.1 3036126 3037010 D cytoplasmic protein; function unknown but contains oxidoreductase domain; hypothetical protein 3036126..3037010 Mycobacterium ulcerans Agy99 4549678 YP_906501.1 CDS MUL_2717 NC_008611.1 3037007 3037741 D hypothetical protein 3037007..3037741 Mycobacterium ulcerans Agy99 4549679 YP_906502.1 CDS MUL_2718 NC_008611.1 3037970 3039103 D PPE61; membrane protein; PPE family protein 3037970..3039103 Mycobacterium ulcerans Agy99 4549681 YP_906503.1 CDS cdh NC_008611.1 3039179 3039973 R secreted protein; involved in phospholipid biosynthesis [catalytic activity: CDP-diacylglycerol + H(2)O = CMP + phosphatidate]; CDP-diacylglycerol pyrophosphatase complement(3039179..3039973) Mycobacterium ulcerans Agy99 4549682 YP_906504.1 CDS MUL_2720 NC_008611.1 3040184 3040783 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(3040184..3040783) Mycobacterium ulcerans Agy99 4549683 YP_906505.1 CDS MUL_2721 NC_008611.1 3040780 3041373 R cytoplasmic protein; hypothetical protein complement(3040780..3041373) Mycobacterium ulcerans Agy99 4549684 YP_906506.1 CDS MUL_2722 NC_008611.1 3041372 3042619 D hypothetical protein 3041372..3042619 Mycobacterium ulcerans Agy99 4549685 YP_906507.1 CDS MUL_2723 NC_008611.1 3042672 3043667 D cytoplasmic protein; hydrolase 3042672..3043667 Mycobacterium ulcerans Agy99 4549686 YP_906508.1 CDS MUL_2724 NC_008611.1 3043739 3044038 R membrane protein; hypothetical protein complement(3043739..3044038) Mycobacterium ulcerans Agy99 4549687 YP_906509.1 CDS MUL_2725 NC_008611.1 3044485 3045327 D cytoplasmic protein; hypothetical protein 3044485..3045327 Mycobacterium ulcerans Agy99 4552879 YP_906510.1 CDS MUL_2726 NC_008611.1 3045590 3046669 D cytoplasmic protein; hypothetical protein 3045590..3046669 Mycobacterium ulcerans Agy99 4552880 YP_906511.1 CDS MUL_2727 NC_008611.1 3046737 3047936 D cytoplasmic protein; aminotransferase 3046737..3047936 Mycobacterium ulcerans Agy99 4552881 YP_906512.1 CDS MUL_2728 NC_008611.1 3047940 3048485 R cytoplasmic protein; putative regulatory protein complement(3047940..3048485) Mycobacterium ulcerans Agy99 4552882 YP_906513.1 CDS MUL_2729 NC_008611.1 3048509 3049468 D membrane protein; hypothetical protein 3048509..3049468 Mycobacterium ulcerans Agy99 4552883 YP_906514.1 CDS cysQ_1 NC_008611.1 3049487 3050215 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; could help control the pool of 3'-phosphoadenoside 5'-phosphosulfate, or its use in sulfite synthesis (by similarity); monophosphatase CysQ complement(3049487..3050215) Mycobacterium ulcerans Agy99 4552884 YP_906515.1 CDS MUL_2734 NC_008611.1 3053998 3055044 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(3053998..3055044) Mycobacterium ulcerans Agy99 4552889 YP_906516.1 CDS MUL_2735 NC_008611.1 3055895 3057229 D cytoplasmic protein; required for the transposition of the insertion element IS2606; transposase for IS2606 3055895..3057229 Mycobacterium ulcerans Agy99 4552890 YP_906517.1 CDS MUL_2736 NC_008611.1 3057353 3058399 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(3057353..3058399) Mycobacterium ulcerans Agy99 4552891 YP_906518.1 CDS MUL_5108 NC_008611.1 3059209 3059388 D potential pseuodgene; cytoplasmic protein; transposase 3059209..3059388 Mycobacterium ulcerans Agy99 4552892 YP_906519.1 CDS MUL_2737 NC_008611.1 3060048 3061193 R PPE51_1; detected in the membranr fraction by proteomics.; membrane protein; PPE family protein complement(3060048..3061193) Mycobacterium ulcerans Agy99 4552893 YP_906520.1 CDS MUL_2738 NC_008611.1 3061983 3062960 R cytoplasmic protein; function unknown but contains metal-dependant hydrolase domain identity; hypothetical protein complement(3061983..3062960) Mycobacterium ulcerans Agy99 4552895 YP_906521.1 CDS MUL_2739 NC_008611.1 3062991 3063947 D cytoplasmic protein; function unknown but has domain identity to UbiE, a methylase involved in ubiquinone/menaquinone biosynthesis; methylase 3062991..3063947 Mycobacterium ulcerans Agy99 4552896 YP_906522.1 CDS MUL_2742 NC_008611.1 3065334 3066116 D cytoplasmic protein; hypothetical protein 3065334..3066116 Mycobacterium ulcerans Agy99 4552898 YP_906523.1 CDS MUL_2743 NC_008611.1 3066144 3068777 R cytoplasmic protein; involved in transcriptional mechanism.; LuxR family transcriptional regulator complement(3066144..3068777) Mycobacterium ulcerans Agy99 4552899 YP_906524.1 CDS htpG NC_008611.1 3068982 3070928 D molecular chaperone; heat shock protein 90 3068982..3070928 Mycobacterium ulcerans Agy99 4552900 YP_906525.1 CDS MUL_2745 NC_008611.1 3070929 3071900 R cytoplasmic protein; hypothetical protein complement(3070929..3071900) Mycobacterium ulcerans Agy99 4552901 YP_906526.1 CDS MUL_2746 NC_008611.1 3072017 3072634 R cytoplasmic protein; probably involved in a transcriptional mechanism; TetR family transcriptional regulator complement(3072017..3072634) Mycobacterium ulcerans Agy99 4552902 YP_906527.1 CDS rubB_1 NC_008611.1 3072866 3073051 R cytoplasmic protein; involved in the hydrocarbon hydroxylating system to convert conversion of dodecane to lauric acid, which transfers electrons from NADH to rubredoxin reductase and then through rubredoxin to alkane 1 monooxygenase.; rubredoxin RubB complement(3072866..3073051) Mycobacterium ulcerans Agy99 4552903 YP_906528.1 CDS rubA_1 NC_008611.1 3073053 3073223 R cytoplasmic protein; involved in the hydrocarbon hydroxylating system, which transfers electrons from NADH to rubredoxin reductase and then through rubredoxin to alkane 1 monooxygenase.; rubredoxin RubA complement(3073053..3073223) Mycobacterium ulcerans Agy99 4552904 YP_906529.1 CDS alkB_1 NC_008611.1 3073220 3074440 R membrane protein; thought to be involved in fatty acid metabolism. generates octanol and oxidized rubredoxin from octane and reduced rubredoxin. also hydroxylates fatty acids in the omega-position [catalytic activity: octane + reduced rubredoxin + (O)2 = 1-octanol + oxidized rubredoxin + H(2)O]; transmembrane alkane 1-monooxygenase AlkB complement(3073220..3074440) Mycobacterium ulcerans Agy99 4552905 YP_906530.1 CDS MUL_2751 NC_008611.1 3076597 3077931 R cytoplasmic protein; required for the transposition of the insertion element IS2606; transposase for IS2606 complement(3076597..3077931) Mycobacterium ulcerans Agy99 4552907 YP_906531.1 CDS MUL_2753 NC_008611.1 3078428 3079144 D cytoplasmic protein; function unknown, probably involved in cellular metabolism, possibly electron transfer.; oxidoreductase 3078428..3079144 Mycobacterium ulcerans Agy99 4552908 YP_906532.1 CDS MUL_2755 NC_008611.1 3079392 3080012 D cytoplasmic protein; cellular metabolism; oxidoreductase 3079392..3080012 Mycobacterium ulcerans Agy99 4552909 YP_906533.1 CDS MUL_2756 NC_008611.1 3080026 3081054 R cytoplasmic protein; acyl-CoA dehydrogenase complement(3080026..3081054) Mycobacterium ulcerans Agy99 4552910 YP_906534.1 CDS MUL_2757 NC_008611.1 3081051 3082229 R cytoplasmic protein; acyl-CoA dehydrogenase complement(3081051..3082229) Mycobacterium ulcerans Agy99 4552911 YP_906535.1 CDS echA8_5 NC_008611.1 3082229 3083026 R Also detected in the membrane fraction by proteomics.; cytoplasmic protein; oxidizes fatty acids using specific components (by similarity) [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]; enoyl-CoA hydratase, EchA8_5 complement(3082229..3083026) Mycobacterium ulcerans Agy99 4552912 YP_906536.1 CDS MUL_2761 NC_008611.1 3083950 3084438 R secreted protein; hypothetical protein complement(3083950..3084438) Mycobacterium ulcerans Agy99 4552914 YP_906537.1 CDS MUL_2762 NC_008611.1 3084623 3085594 R cytoplasmic protein; involved in transcriptional mechanism.; IclR family transcriptional regulator complement(3084623..3085594) Mycobacterium ulcerans Agy99 4552915 YP_906538.1 CDS MUL_2763 NC_008611.1 3085932 3086537 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(3085932..3086537) Mycobacterium ulcerans Agy99 4552917 YP_906539.1 CDS MUL_2765 NC_008611.1 3087587 3088222 R cytoplasmic protein; methyltransferase (methylase) complement(3087587..3088222) Mycobacterium ulcerans Agy99 4552919 YP_906540.1 CDS MUL_2766 NC_008611.1 3088219 3089151 R cytoplasmic protein; function unknown but high domain identity with O- Methyltransferases involved in polyketide biosynthesis; O-methyltransferase complement(3088219..3089151) Mycobacterium ulcerans Agy99 4552920 YP_906541.1 CDS MUL_2767 NC_008611.1 3089265 3089681 D membrane protein; hypothetical protein 3089265..3089681 Mycobacterium ulcerans Agy99 4552921 YP_906542.1 CDS MUL_2768 NC_008611.1 3089761 3090603 D cytoplasmic protein; function unknown, but high domain identity with epimerases and phenazine biosynthesis-like protein, PhzC/PhzF which is involved in dimerisation of two 2,3- dihydro-3-oxo-anthranilic acid molecules to create Pca by P. fluorescens.; hypothetical protein 3089761..3090603 Mycobacterium ulcerans Agy99 4552922 YP_906543.1 CDS MUL_2769 NC_008611.1 3090835 3092166 R cytoplasmic protein; hypothetical protein complement(3090835..3092166) Mycobacterium ulcerans Agy99 4552923 YP_906544.1 CDS MUL_2770 NC_008611.1 3092159 3093346 R membrane protein; hypothetical protein complement(3092159..3093346) Mycobacterium ulcerans Agy99 4552924 YP_906545.1 CDS MUL_2772 NC_008611.1 3094565 3095197 D secreted protein; function unknown but has ribonuclease I domain identity; hypothetical protein 3094565..3095197 Mycobacterium ulcerans Agy99 4552926 YP_906546.1 CDS MUL_2773 NC_008611.1 3095672 3096592 D membrane protein; function unknown but domain identity with mechanosensitive ion channels; hypothetical protein 3095672..3096592 Mycobacterium ulcerans Agy99 4552927 YP_906547.1 CDS MUL_2774 NC_008611.1 3096693 3099638 R Detected in the membrane fraction by proteomics (LC- MS/MS) Also detected in the cytoplasmic fraction by 2D-LC- MS/MS; membrane protein; function unknown but contains multiple functional domains including vWA domain (von willebrand factor (vWF) type a domain; found in extracellular proteins, like integrins, and mediates adhesion. also contains a vault protein inter-alpha-trypsin domain; hypothetical protein complement(3096693..3099638) Mycobacterium ulcerans Agy99 4552928 YP_906548.1 CDS MUL_2775 NC_008611.1 3099709 3100491 D cytoplasmic protein; hypothetical protein 3099709..3100491 Mycobacterium ulcerans Agy99 4552929 YP_906549.1 CDS MUL_2778 NC_008611.1 3102481 3103527 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(3102481..3103527) Mycobacterium ulcerans Agy99 4552947 YP_906550.1 CDS MUL_2781 NC_008611.1 3104935 3105666 D cytoplasmic protein; hypothetical protein 3104935..3105666 Mycobacterium ulcerans Agy99 4552949 YP_906551.1 CDS MUL_2782 NC_008611.1 3106065 3108242 D cytoplasmic protein; function unknown but contains glutamine synthetase catalytic domain; hypothetical protein 3106065..3108242 Mycobacterium ulcerans Agy99 4552950 YP_906552.1 CDS MUL_2783 NC_008611.1 3108511 3109791 R PE_PGRS39; membrane protein; PE-PGRS family protein complement(3108511..3109791) Mycobacterium ulcerans Agy99 4552951 YP_906553.1 CDS MUL_2784 NC_008611.1 3110256 3110828 D secreted protein; proline, glycine, valine-rich secreted protein 3110256..3110828 Mycobacterium ulcerans Agy99 4552952 YP_906554.1 CDS MUL_2786 NC_008611.1 3112125 3112883 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; alanine rich hydrolase 3112125..3112883 Mycobacterium ulcerans Agy99 4552954 YP_906555.1 CDS MUL_2787 NC_008611.1 3112900 3114675 R membrane protein; function unknown. possibly transporter involved in transport of undeterminated substrate (possibly cationic amino acids) across the membrane: so responsible for the translocation of the substrate across the membrane.; hypothetical protein complement(3112900..3114675) Mycobacterium ulcerans Agy99 4552955 YP_906556.1 CDS MUL_2788 NC_008611.1 3114759 3115976 R cytoplasmic protein; function unknown, high domain identity with saccharopine dehydrogenase. this family comprises three structural domains that can not be separated in the linear sequence. in some organisms this enzyme is found as a bifunctional polypeptide with lysine ketoglutarate.; saccharopine dehydrogenase complement(3114759..3115976) Mycobacterium ulcerans Agy99 4552956 YP_906557.1 CDS MUL_2789 NC_008611.1 3116266 3117756 R catalyzes the formation of 4-aminobutanoate from 4-aminobutanal; involved in putrescine degradation; gamma-aminobutyraldehyde dehydrogenase complement(3116266..3117756) Mycobacterium ulcerans Agy99 4552957 YP_906558.1 CDS MUL_2790 NC_008611.1 3118064 3118393 D cytoplasmic protein; involved in transcriptional mechanism.; Lrp/AsnC family transcriptional regulator 3118064..3118393 Mycobacterium ulcerans Agy99 4552958 YP_906559.1 CDS MUL_2791 NC_008611.1 3118390 3119745 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); membrane protein; function unknown, high domain identity with BioA, aminotransferase [coenzyme metabolism]; hypothetical protein 3118390..3119745 Mycobacterium ulcerans Agy99 4552959 YP_906560.1 CDS MUL_2792 NC_008611.1 3119720 3120700 R cytoplasmic protein; function unknown - possible oxidoreducatse; hypothetical protein complement(3119720..3120700) Mycobacterium ulcerans Agy99 4552960 YP_906561.1 CDS MUL_2793 NC_008611.1 3120805 3121371 R cytoplasmic protein; possibly involved in transcriptional mechanism.; transcriptional regulatory protein complement(3120805..3121371) Mycobacterium ulcerans Agy99 4552961 YP_906562.1 CDS MUL_2794 NC_008611.1 3121486 3122331 D function unknown, probably involved in cellular metabolism; short chain dehydrogenase 3121486..3122331 Mycobacterium ulcerans Agy99 4552962 YP_906563.1 CDS MUL_2795 NC_008611.1 3122362 3122790 D cytoplasmic protein; hypothetical protein 3122362..3122790 Mycobacterium ulcerans Agy99 4552963 YP_906564.1 CDS MUL_2796 NC_008611.1 3122787 3123215 R cytoplasmic protein; rifampin ADP-ribosyl transferase complement(3122787..3123215) Mycobacterium ulcerans Agy99 4552964 YP_906565.1 CDS qor_1 NC_008611.1 3123217 3124440 R cytoplasmic protein; catalyzes the one electron reduction of certain quinones [catalytic activity: NADPH + quinone = NADP+ + semiquinone]; quinone reductase, Qor complement(3123217..3124440) Mycobacterium ulcerans Agy99 4552965 YP_906566.1 CDS amiC_1 NC_008611.1 3124478 3125914 D membrane protein; hydrolyzes a monocarboxylic acid amide and generates a monocarboxylate [catalytic activity: a monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)]; amidase 3124478..3125914 Mycobacterium ulcerans Agy99 4552966 YP_906567.1 CDS plcB NC_008611.1 3125957 3127492 R membrane protein; hydrolyzes sphingomyelin in addition to phosphatidylcholine. probable virulence factor implicated in the pathogenesis of mycobacterium tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity [catalytic activity: a phosphatidylcholine + H(2)O = 1,2- diacylglycerol + choline phosphate]; membrane-associated phospholipase C2 PlcB complement(3125957..3127492) Mycobacterium ulcerans Agy99 4552967 YP_906568.1 CDS MUL_2802 NC_008611.1 3129318 3130121 R cytoplasmic protein; function unknown but domain identity with streptomyces scabies esterase protein; hydrolase complement(3129318..3130121) Mycobacterium ulcerans Agy99 4552970 YP_906569.1 CDS thiG NC_008611.1 3130118 3130912 R functions in thiamine (vitamin B1) biosynthesis; in Bacillus subtilis this enzyme catalyzes the formation of thiazole from dehydroxyglycine and 1-deoxy-D-xylulose-5-phosphate and ThiS-thiocarboxylate; thiazole synthase complement(3130118..3130912) Mycobacterium ulcerans Agy99 4552971 YP_906570.1 CDS thiS NC_008611.1 3130905 3131102 R cytoplasmic protein; part of the thiamine biosynthesis operon. ThiS (thiamins) is a 66 aa protein involved in sulphur transfer. this is coded in the thiCEFSGH operon in E. coli.; thiamine biosynthesis protein ThiS complement(3130905..3131102) Mycobacterium ulcerans Agy99 4552972 YP_906571.1 CDS thiO NC_008611.1 3131099 3132118 R membrane protein; involved in thiamine biosynthesis.; thiamine biosynthesis oxidoreductase ThiO complement(3131099..3132118) Mycobacterium ulcerans Agy99 4552973 YP_906572.1 CDS thiE NC_008611.1 3132280 3132942 D Condenses 4-methyl-5-(beta-hydroxyethyl)-thiazole monophosphate and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate to form thiamine monophosphate; thiamine-phosphate pyrophosphorylase 3132280..3132942 Mycobacterium ulcerans Agy99 4552974 YP_906573.1 CDS mutT3 NC_008611.1 3132914 3133537 R cytoplasmic protein; possibly involved in the go system responsible for removing an oxidatively damaged form of guanine (7,8- dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8- oxo-dGTP is inserted opposite da and dc residues of template DNA with almost equal efficiency thus leading to a.T to G.C transversions. MutT specifically degrades 8-oxo- dGTP to the monophosphate [catalytic activity: 8-oxo-dGTP + H2O = 8-oxo-DGMP + pyrophosphate]; mutator protein MutT3 complement(3132914..3133537) Mycobacterium ulcerans Agy99 4552975 YP_906574.1 CDS MUL_2808 NC_008611.1 3133689 3135008 D membrane protein; hypothetical protein 3133689..3135008 Mycobacterium ulcerans Agy99 4552976 YP_906575.1 CDS glnH NC_008611.1 3135008 3135994 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in active transport of glutamine across the membrane (import) interacts with the glutamine- transport system.; glutamine-binding lipoprotein GlnH 3135008..3135994 Mycobacterium ulcerans Agy99 4552977 YP_906576.1 CDS pknG NC_008611.1 3136099 3138297 D Detected in the secreted protein fraction by proteomics.; membrane protein; involved in signal transduction (via phosphorylation) thought to regulate amino-acid uptake and stationary-phase metabolism. phosphorylates the peptide substrate myelin basic protein (MBP) at serine residues [catalytic activity: ATP + a protein = ADP + a P; serine/threonine-protein kinase PknG 3136099..3138297 Mycobacterium ulcerans Agy99 4552978 YP_906577.1 CDS MUL_2811 NC_008611.1 3138422 3139282 R cytoplasmic protein; hypothetical protein complement(3138422..3139282) Mycobacterium ulcerans Agy99 4552979 YP_906578.1 CDS ackA NC_008611.1 3139461 3140600 R AckA utilizes acetate and can acetylate CheY which increases signal strength during flagellar rotation; utilizes magnesium and ATP; also involved in conversion of acetate to aceyl-CoA; acetate kinase complement(3139461..3140600) Mycobacterium ulcerans Agy99 4552980 YP_906579.1 CDS fgd1 NC_008611.1 3142710 3143720 R cytoplasmic protein; catalyzes oxidation of glucose-6-phosphate to 6- phosphogluconolactone using coenzyme F420 (an *-hydroxy-5- deazaflavin derivative) as the electron acceptor.; F420-dependent glucose-6-phosphate dehydrogenase Fgd1 complement(3142710..3143720) Mycobacterium ulcerans Agy99 4552982 YP_906580.1 CDS MUL_2815 NC_008611.1 3143802 3144554 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; beta lactamase-like protein 3143802..3144554 Mycobacterium ulcerans Agy99 4552983 YP_906581.1 CDS MUL_2821 NC_008611.1 3150532 3151578 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(3150532..3151578) Mycobacterium ulcerans Agy99 4552989 YP_906582.1 CDS MUL_2823 NC_008611.1 3152758 3153264 R cytoplasmic protein; function unknown but has domain identity with acetyltransferases; hypothetical protein complement(3152758..3153264) Mycobacterium ulcerans Agy99 4552991 YP_906583.1 CDS MUL_2824 NC_008611.1 3153438 3153818 R membrane protein; hypothetical protein complement(3153438..3153818) Mycobacterium ulcerans Agy99 4552992 YP_906584.1 CDS fadE7 NC_008611.1 3153854 3155041 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE7 3153854..3155041 Mycobacterium ulcerans Agy99 4552993 YP_906585.1 CDS MUL_2827 NC_008611.1 3156266 3157600 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 3156266..3157600 Mycobacterium ulcerans Agy99 4552995 YP_906586.1 CDS MUL_2828 NC_008611.1 3157724 3158770 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(3157724..3158770) Mycobacterium ulcerans Agy99 4552996 YP_906587.1 CDS MUL_2830 NC_008611.1 3159790 3159897 D cytoplasmic protein; hypothetical protein 3159790..3159897 Mycobacterium ulcerans Agy99 4552998 YP_906588.1 CDS MUL_2831 NC_008611.1 3160003 3160281 D cytoplasmic protein; hypothetical protein 3160003..3160281 Mycobacterium ulcerans Agy99 4552999 YP_906589.1 CDS lipU_1 NC_008611.1 3160374 3161438 D Part of a 2158bp section not present in M. marinum M strain. However LipU orthologs are found in MTB and M. avium paratb; membrane protein; function unknown but probably involved in lipid hydrolysis; lipase LipU 3160374..3161438 Mycobacterium ulcerans Agy99 4553016 YP_906590.1 CDS MUL_2833 NC_008611.1 3161464 3162093 R cytoplasmic protein; hypothetical protein complement(3161464..3162093) Mycobacterium ulcerans Agy99 4553017 YP_906591.1 CDS purA NC_008611.1 3162090 3163388 R catalyzes the formation of N6-(1,2,-dicarboxyethyl)-AMP from L-aspartate, inosine monophosphate and GTP in AMP biosynthesis; adenylosuccinate synthetase complement(3162090..3163388) Mycobacterium ulcerans Agy99 4553018 YP_906592.1 CDS MUL_2835 NC_008611.1 3163479 3164156 D cytoplasmic protein; hypothetical protein 3163479..3164156 Mycobacterium ulcerans Agy99 4553019 YP_906593.1 CDS MUL_2836 NC_008611.1 3164167 3164946 D membrane protein; hypothetical protein 3164167..3164946 Mycobacterium ulcerans Agy99 4553020 YP_906594.1 CDS MUL_2837 NC_008611.1 3164991 3165914 D membrane protein; function unknown, possibly involved in transport of metal ions across the membrane.; efflux membrane protein 3164991..3165914 Mycobacterium ulcerans Agy99 4553021 YP_906595.1 CDS MUL_2838 NC_008611.1 3165944 3166918 R cytoplasmic protein; involved in sulfation: activity towards typical ceramide glycolipids and trehalose glycolipids.; glycolipid sulfotransferase complement(3165944..3166918) Mycobacterium ulcerans Agy99 4553022 YP_906596.1 CDS MUL_5105 NC_008611.1 3166919 3167056 R cytoplasmic protein; hypothetical protein complement(3166919..3167056) Mycobacterium ulcerans Agy99 4553023 YP_906597.1 CDS MUL_2839 NC_008611.1 3167060 3167314 R cytoplasmic protein; hypothetical protein complement(3167060..3167314) Mycobacterium ulcerans Agy99 4553024 YP_906598.1 CDS MUL_2840 NC_008611.1 3167335 3168093 R cytoplasmic protein; hypothetical protein complement(3167335..3168093) Mycobacterium ulcerans Agy99 4553025 YP_906599.1 CDS MUL_2841 NC_008611.1 3168317 3168739 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(3168317..3168739) Mycobacterium ulcerans Agy99 4553026 YP_906600.1 CDS MUL_2842 NC_008611.1 3168881 3169696 D Detected in the membrane fraction by proteomics (LC- MS/MS) Also detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; hypothetical protein 3168881..3169696 Mycobacterium ulcerans Agy99 4553027 YP_906601.1 CDS bioF2_3 NC_008611.1 3169801 3170475 R cytoplasmic protein; could be involved in biotin biosynthesis (at the first step) [catalytic activity: 6-carboxyhexanoyl-CoA + L- alanine = 8-amino-7-oxononanoate + CoA + CO2]; 8-amino-7-oxononanoate synthase complement(3169801..3170475) Mycobacterium ulcerans Agy99 4553028 YP_906602.1 CDS MUL_2844 NC_008611.1 3170669 3171715 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(3170669..3171715) Mycobacterium ulcerans Agy99 4553029 YP_906603.1 CDS MUL_2846 NC_008611.1 3174525 3175073 R cytoplasmic protein; hypothetical protein complement(3174525..3175073) Mycobacterium ulcerans Agy99 4553031 YP_906604.1 CDS MUL_2847 NC_008611.1 3175082 3175687 R cytoplasmic protein; GTP/ATP-binding protein complement(3175082..3175687) Mycobacterium ulcerans Agy99 4553032 YP_906605.1 CDS MUL_2848 NC_008611.1 3175668 3175943 R cytoplasmic protein; hypothetical protein complement(3175668..3175943) Mycobacterium ulcerans Agy99 4553033 YP_906606.1 CDS MUL_2849 NC_008611.1 3176030 3176461 R cytoplasmic protein; hypothetical protein complement(3176030..3176461) Mycobacterium ulcerans Agy99 4553034 YP_906607.1 CDS MUL_2850 NC_008611.1 3176458 3179193 R membrane protein; function unknown but may be a regulatory protein as it contains a signal transduction histidine kinase domain; putative regultory protein complement(3176458..3179193) Mycobacterium ulcerans Agy99 4553035 YP_906608.1 CDS MUL_2851 NC_008611.1 3179410 3180015 R cytoplasmic protein; function unknown but may be involved in RNA methylation; methyltransferase complement(3179410..3180015) Mycobacterium ulcerans Agy99 4553036 YP_906609.1 CDS spoU NC_008611.1 3180083 3180547 D cytoplasmic protein; involved in rRNA methylation; tRNA/rRNA methyltransferase SpoU 3180083..3180547 Mycobacterium ulcerans Agy99 4553037 YP_906610.1 CDS MUL_2853 NC_008611.1 3180728 3181936 D PE_PGRS51; membrane protein; PE-PGRS family protein 3180728..3181936 Mycobacterium ulcerans Agy99 4553038 YP_906611.1 CDS MUL_2854 NC_008611.1 3181948 3182592 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); cytoplasmic protein; function unknown, probably involved in cellular metabolism; nitroreductase complement(3181948..3182592) Mycobacterium ulcerans Agy99 4553039 YP_906612.1 CDS MUL_2855 NC_008611.1 3182933 3183979 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3182933..3183979 Mycobacterium ulcerans Agy99 4553040 YP_906613.1 CDS MUL_2856 NC_008611.1 3184204 3184521 R hypothetical protein complement(3184204..3184521) Mycobacterium ulcerans Agy99 4553042 YP_906614.1 CDS MUL_2857 NC_008611.1 3184630 3184899 R cytoplasmic protein; this protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.; iron-regulated elongation factor Tu Tuf-like complement(3184630..3184899) Mycobacterium ulcerans Agy99 4553043 YP_906615.1 CDS MUL_2858 NC_008611.1 3184889 3185332 D cytoplasmic protein; hypothetical protein 3184889..3185332 Mycobacterium ulcerans Agy99 4553044 YP_906616.1 CDS MUL_2860 NC_008611.1 3186250 3187296 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(3186250..3187296) Mycobacterium ulcerans Agy99 4553046 YP_906617.1 CDS MUL_2861 NC_008611.1 3187661 3187948 R hypothetical protein complement(3187661..3187948) Mycobacterium ulcerans Agy99 4553047 YP_906618.1 CDS MUL_2862 NC_008611.1 3188463 3188753 R cytoplasmic protein; function unknown, probably involved in cellular metabolism; oxidoreductase complement(3188463..3188753) Mycobacterium ulcerans Agy99 4553049 YP_906619.1 CDS MUL_2863 NC_008611.1 3188834 3189406 R membrane protein; Mce associated membrane protein complement(3188834..3189406) Mycobacterium ulcerans Agy99 4553095 YP_906620.1 CDS MUL_2864 NC_008611.1 3189403 3189966 R membrane protein; Mce associated membrane protein complement(3189403..3189966) Mycobacterium ulcerans Agy99 4553096 YP_906621.1 CDS mce3F NC_008611.1 3190010 3191473 R membrane protein; function unknown, but thought involved in host cell invasion.; MCE-family protein Mce3F complement(3190010..3191473) Mycobacterium ulcerans Agy99 4553097 YP_906622.1 CDS lprM NC_008611.1 3191474 3192643 R membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE family lipoprotein LprM complement(3191474..3192643) Mycobacterium ulcerans Agy99 4553098 YP_906623.1 CDS mce3D NC_008611.1 3192643 3193926 R membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE-family protein Mce3D complement(3192643..3193926) Mycobacterium ulcerans Agy99 4553099 YP_906624.1 CDS mce3B NC_008611.1 3195203 3196231 R membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE-family protein Mce3B complement(3195203..3196231) Mycobacterium ulcerans Agy99 4553101 YP_906625.1 CDS yrb3B NC_008611.1 3197697 3198389 R membrane protein; function unknown, but probably part of Mce3 operon and member of YrbE family; integral membrane protein, Yrb3B complement(3197697..3198389) Mycobacterium ulcerans Agy99 4553103 YP_906626.1 CDS yrbE3A NC_008611.1 3198561 3199394 R membrane protein; integral membrane protein YrbE3A complement(3198561..3199394) Mycobacterium ulcerans Agy99 4553104 YP_906627.1 CDS MUL_2873 NC_008611.1 3199719 3199913 R cytoplasmic protein; ferredoxin complement(3199719..3199913) Mycobacterium ulcerans Agy99 4553105 YP_906628.1 CDS MUL_2874 NC_008611.1 3199910 3200266 R cytoplasmic protein; function unknown, domain identity with ketosteroid isomerase-related proteins; hypothetical protein complement(3199910..3200266) Mycobacterium ulcerans Agy99 4553106 YP_906629.1 CDS MUL_2876 NC_008611.1 3201681 3202916 D cytoplasmic protein; function unknown but weak domain identity with two- component response regulators; putative regulatory protein 3201681..3202916 Mycobacterium ulcerans Agy99 4553108 YP_906630.1 CDS MUL_2877 NC_008611.1 3202919 3204442 R cytoplasmic protein; thought to oxidize a wide variety of aliphatic and aromatic aldehydes.; aldehyde dehydrogenase complement(3202919..3204442) Mycobacterium ulcerans Agy99 4553109 YP_906631.1 CDS MUL_2878 NC_008611.1 3204439 3204858 R cytoplasmic protein; hypothetical protein complement(3204439..3204858) Mycobacterium ulcerans Agy99 4553110 YP_906632.1 CDS mce3R NC_008611.1 3205341 3206543 D cytoplasmic protein; involved in transcriptional mechanism; repression of the Mce3 operon. could also have a regulatory action on the Mce2 operon.; TetR family transcriptional regulator 3205341..3206543 Mycobacterium ulcerans Agy99 4553111 YP_906633.1 CDS MUL_2881 NC_008611.1 3206870 3207232 R membrane protein; maybe required for the translocation of the substrate across the membrane.; multidrugs-transport integral membrane protein Mmr-like complement(3206870..3207232) Mycobacterium ulcerans Agy99 4553112 YP_906634.1 CDS MUL_2882 NC_008611.1 3207567 3207887 R cytoplasmic protein; hypothetical protein complement(3207567..3207887) Mycobacterium ulcerans Agy99 4553113 YP_906635.1 CDS MUL_2885 NC_008611.1 3208993 3209763 R function unknown, probably involved in cellular metabolism; short-chain type dehydrogenase/reductase complement(3208993..3209763) Mycobacterium ulcerans Agy99 4553314 YP_906636.1 CDS ribA1 NC_008611.1 3209767 3210864 R cytoplasmic protein; involved in riboflavin biosynthesis [catalytic activity : GTP + 3 H(2)O = formate + 2,5-diamino-6-hydroxy- 4-(5-phosphoribosylamino)pyrimidine + diphosphate]; riboflavin biosynthesis protein RibA1 complement(3209767..3210864) Mycobacterium ulcerans Agy99 4553315 YP_906637.1 CDS MUL_2887 NC_008611.1 3210861 3211394 R cytoplasmic protein; function unknown, probably involved in cellular metabolism; oxidoreducatse complement(3210861..3211394) Mycobacterium ulcerans Agy99 4553316 YP_906638.1 CDS ephB NC_008611.1 3211391 3212446 R cytoplasmic protein; this enzyme acts on epoxides (alkene oxides, oxiranes) and Arene oxides. plays a role in xenobiotic metabolism by degrading potential toxic epoxides. also determines steady-state levels of physiological mediators.; epoxide hydrolase EphB complement(3211391..3212446) Mycobacterium ulcerans Agy99 4553317 YP_906639.1 CDS MUL_2889 NC_008611.1 3212458 3214977 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown, may be involved in electron transfer.; oxygenase complement(3212458..3214977) Mycobacterium ulcerans Agy99 4553318 YP_906640.1 CDS MUL_2890 NC_008611.1 3215005 3216141 R cytoplasmic protein; function unknown, probably involved in cellular metabolism; oxygenase complement(3215005..3216141) Mycobacterium ulcerans Agy99 4553319 YP_906641.1 CDS fadE17 NC_008611.1 3217401 3218573 D cytoplasmic protein; function unknown, but supposed involvement in lipid degradation.; acyl-CoA dehydrogenase FadE17 3217401..3218573 Mycobacterium ulcerans Agy99 4553321 YP_906642.1 CDS fadE18 NC_008611.1 3218575 3219663 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown, but supposed involvement in lipid degradation.; acyl-CoA dehydrogenase FadE18 3218575..3219663 Mycobacterium ulcerans Agy99 4553322 YP_906643.1 CDS tpx NC_008611.1 3219685 3220182 R antioxidant activity; thioredoxin-dependent thiol peroxidase; forms homodimers in solution; shows substrate specificity to alkyl hydroperoxides; periplasmic protein; thiol peroxidase complement(3219685..3220182) Mycobacterium ulcerans Agy99 4553323 YP_906644.1 CDS MUL_2895 NC_008611.1 3220328 3221371 R cytoplasmic protein; function unknown but domain identity with Fe-S oxidoreductases; oxidoreductase complement(3220328..3221371) Mycobacterium ulcerans Agy99 4553324 YP_906645.1 CDS MUL_2896 NC_008611.1 3221415 3222461 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(3221415..3222461) Mycobacterium ulcerans Agy99 4553325 YP_906646.1 CDS MUL_2898 NC_008611.1 3223035 3224369 R cytoplasmic protein; required for the transposition of the insertion element IS2606; transposase for IS2606 complement(3223035..3224369) Mycobacterium ulcerans Agy99 4553327 YP_906647.1 CDS MUL_5111 NC_008611.1 3224436 3224819 R cytoplasmic protein; hypothetical protein complement(3224436..3224819) Mycobacterium ulcerans Agy99 4553328 YP_906648.1 CDS MUL_2901 NC_008611.1 3226070 3227116 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3226070..3227116 Mycobacterium ulcerans Agy99 4553330 YP_906649.1 CDS MUL_2902 NC_008611.1 3228220 3229170 D cytoplasmic protein; involved in transcriptional mechanism; transcriptional regulatory protein 3228220..3229170 Mycobacterium ulcerans Agy99 4553331 YP_906650.1 CDS MUL_2904 NC_008611.1 3229990 3230454 R membrane protein; hypothetical protein complement(3229990..3230454) Mycobacterium ulcerans Agy99 4553333 YP_906651.1 CDS MUL_2905 NC_008611.1 3230552 3231112 D cytoplasmic protein; function unknown but contains dihydrofolate reductase domain; hypothetical protein 3230552..3231112 Mycobacterium ulcerans Agy99 4553334 YP_906652.1 CDS MUL_2906 NC_008611.1 3231143 3232027 R cytoplasmic protein; function unknown but has weak indentity with hydrolase domains; hypothetical protein complement(3231143..3232027) Mycobacterium ulcerans Agy99 4553335 YP_906653.1 CDS MUL_2907 NC_008611.1 3232029 3232505 R cytoplasmic protein; hypothetical protein complement(3232029..3232505) Mycobacterium ulcerans Agy99 4553336 YP_906654.1 CDS MUL_2909 NC_008611.1 3233381 3234154 R cytoplasmic protein; hypothetical protein complement(3233381..3234154) Mycobacterium ulcerans Agy99 4553338 YP_906655.1 CDS MUL_2910 NC_008611.1 3234251 3234664 R cytoplasmic protein; hypothetical protein complement(3234251..3234664) Mycobacterium ulcerans Agy99 4553339 YP_906656.1 CDS MUL_2911 NC_008611.1 3234735 3236204 R cytoplasmic protein; function unknown: involved in carbohydrate transport and metabolism; alpha-L-fucosidase complement(3234735..3236204) Mycobacterium ulcerans Agy99 4553340 YP_906657.1 CDS ahpC_1 NC_008611.1 3236418 3236966 D cytoplasmic protein; involved in oxidative stress response.; alkyl hydroperoxide reductase C protein AhpC 3236418..3236966 Mycobacterium ulcerans Agy99 4553341 YP_906658.1 CDS MUL_2913 NC_008611.1 3236963 3238297 R cytoplasmic protein; required for the transposition of the insertion element IS2606; transposase for IS2606 complement(3236963..3238297) Mycobacterium ulcerans Agy99 4553342 YP_906659.1 CDS MUL_2914 NC_008611.1 3238991 3240355 R catalyzes the transformation of hydroxyatrazine to N-isopropylammelide and ethylamine in the atrazine degradation pathway.; hydroxydechloroatrazine ethylaminohydrolase complement(3238991..3240355) Mycobacterium ulcerans Agy99 4553343 YP_906660.1 CDS MUL_2915 NC_008611.1 3240788 3241834 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3240788..3241834 Mycobacterium ulcerans Agy99 4553344 YP_906661.1 CDS MUL_2916 NC_008611.1 3241943 3242422 D cytoplasmic protein; hypothetical protein 3241943..3242422 Mycobacterium ulcerans Agy99 4553345 YP_906662.1 CDS MUL_2917 NC_008611.1 3242562 3243140 D cytoplasmic protein; hypothetical protein 3242562..3243140 Mycobacterium ulcerans Agy99 4553346 YP_906663.1 CDS fadD31 NC_008611.1 3245086 3246960 R activates fatty acids by binding to coenzyme A; putative fatty-acid--CoA ligase complement(3245086..3246960) Mycobacterium ulcerans Agy99 4553348 YP_906664.1 CDS MUL_2922 NC_008611.1 3248100 3248624 D cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator 3248100..3248624 Mycobacterium ulcerans Agy99 4553350 YP_906665.1 CDS MUL_2924 NC_008611.1 3248804 3249205 D membrane protein; hypothetical protein 3248804..3249205 Mycobacterium ulcerans Agy99 4553352 YP_906666.1 CDS MUL_2925 NC_008611.1 3249499 3249930 D cytoplasmic protein; hypothetical protein 3249499..3249930 Mycobacterium ulcerans Agy99 4553353 YP_906667.1 CDS aao NC_008611.1 3249945 3250928 R cytoplasmic protein; wide specificity for D-amino acids. also acts on glycine [catalytic activity: a D-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2]; D-amino acid oxidase Aao complement(3249945..3250928) Mycobacterium ulcerans Agy99 4553354 YP_906668.1 CDS MUL_2927 NC_008611.1 3250941 3251408 R membrane protein; hypothetical protein complement(3250941..3251408) Mycobacterium ulcerans Agy99 4553355 YP_906669.1 CDS MUL_2928 NC_008611.1 3251457 3251834 R cytoplasmic protein; hypothetical protein complement(3251457..3251834) Mycobacterium ulcerans Agy99 4553356 YP_906670.1 CDS MUL_2929 NC_008611.1 3251870 3252595 R cytoplasmic protein; hypothetical protein complement(3251870..3252595) Mycobacterium ulcerans Agy99 4553357 YP_906671.1 CDS MUL_2930 NC_008611.1 3253335 3254381 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3253335..3254381 Mycobacterium ulcerans Agy99 4553358 YP_906672.1 CDS MUL_2931 NC_008611.1 3254347 3254637 R cytoplasmic protein; hypothetical protein complement(3254347..3254637) Mycobacterium ulcerans Agy99 4553359 YP_906673.1 CDS MUL_2932 NC_008611.1 3254728 3255975 R membrane protein; PPE family protein complement(3254728..3255975) Mycobacterium ulcerans Agy99 4553360 YP_906674.1 CDS MUL_2933 NC_008611.1 3256310 3256909 R cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator complement(3256310..3256909) Mycobacterium ulcerans Agy99 4553361 YP_906675.1 CDS MUL_2934 NC_008611.1 3257024 3258604 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; dehydrogenase 3257024..3258604 Mycobacterium ulcerans Agy99 4553362 YP_906676.1 CDS fadB5 NC_008611.1 3258655 3259659 R cytoplasmic protein; thought to be involved in fatty acid degradation. FadB and FadA are the alpha and BetA subunits of the multifunctional enzyme complex of the fatty acid degradation cycle.; oxidoreductase FadB5 complement(3258655..3259659) Mycobacterium ulcerans Agy99 4553363 YP_906677.1 CDS MUL_2936 NC_008611.1 3259702 3260130 R cytoplasmic protein; hypothetical protein complement(3259702..3260130) Mycobacterium ulcerans Agy99 4553364 YP_906678.1 CDS MUL_2937 NC_008611.1 3260127 3260453 R cytoplasmic protein; involved in transcriptional regulation.; ArsR-type repressor complement(3260127..3260453) Mycobacterium ulcerans Agy99 4553365 YP_906679.1 CDS MUL_2938 NC_008611.1 3260512 3261231 R cytoplasmic protein; hypothetical protein complement(3260512..3261231) Mycobacterium ulcerans Agy99 4553416 YP_906680.1 CDS MUL_2939 NC_008611.1 3261285 3261638 R cytoplasmic protein; hypothetical protein complement(3261285..3261638) Mycobacterium ulcerans Agy99 4553417 YP_906681.1 CDS MUL_2941 NC_008611.1 3262415 3262783 R Detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; hypothetical protein complement(3262415..3262783) Mycobacterium ulcerans Agy99 4553419 YP_906682.1 CDS MUL_2942 NC_008611.1 3262948 3263994 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(3262948..3263994) Mycobacterium ulcerans Agy99 4553420 YP_906683.1 CDS aceAb NC_008611.1 3264294 3266582 D cytoplasmic protein; involved in glyoxylate bypass, an alternative to the tricarboxylic acid cycle [catalytic activity: isocitrate = succinate + glyoxylate]; isocitrate lyase AceAb 3264294..3266582 Mycobacterium ulcerans Agy99 4553421 YP_906684.1 CDS MUL_2948 NC_008611.1 3270518 3270985 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 3270518..3270985 Mycobacterium ulcerans Agy99 4553424 YP_906685.1 CDS MUL_2949 NC_008611.1 3271044 3272207 R Detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; monooxygenase complement(3271044..3272207) Mycobacterium ulcerans Agy99 4553425 YP_906686.1 CDS lipD NC_008611.1 3273357 3274640 D membrane protein; function unknown, lipolytic enzyme probably involved in cellular metabolism; lipase LipD 3273357..3274640 Mycobacterium ulcerans Agy99 4553427 YP_906687.1 CDS MUL_2952 NC_008611.1 3274661 3275410 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; hypothetical protein complement(3274661..3275410) Mycobacterium ulcerans Agy99 4553428 YP_906688.1 CDS MUL_2955 NC_008611.1 3277732 3280677 D membrane protein; involved in (I) tranposition of IS2606 and (II) transport of sialic acid across the membrane. responsible for the translocation of the substrate across the membrane.; fusion protein of transposase for IS2606 and sialic acid-transport integral membrane protein NanT 3277732..3280677 Mycobacterium ulcerans Agy99 4553430 YP_906689.1 CDS cinA NC_008611.1 3280350 3281639 R cytoplasmic protein; CinA is the first gene in the competence-inducible (cin) operon, and is thought to be specifically required at some stage in the process of transformation.; competence damage-inducible protein A complement(3280350..3281639) Mycobacterium ulcerans Agy99 4553431 YP_906690.1 CDS MUL_2957 NC_008611.1 3281744 3281986 R membrane protein; PE-PGRS family protein complement(3281744..3281986) Mycobacterium ulcerans Agy99 4553432 YP_906691.1 CDS lppD NC_008611.1 3282216 3282710 D Truncated wrt to orthologues, missing last 12aa - potential pseudogene; membrane protein; function unknown but contains predicted phosphatase domain; lipoprotein LppD 3282216..3282710 Mycobacterium ulcerans Agy99 4553433 YP_906692.1 CDS MUL_2959 NC_008611.1 3282707 3283033 R cytoplasmic protein; hypothetical protein complement(3282707..3283033) Mycobacterium ulcerans Agy99 4553434 YP_906693.1 CDS MUL_2960 NC_008611.1 3283330 3283761 D hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr); possible defense mechanism against a harmful effect of D-tyrosine; D-tyrosyl-tRNA(Tyr) deacylase 3283330..3283761 Mycobacterium ulcerans Agy99 4553435 YP_906694.1 CDS MUL_2961 NC_008611.1 3283803 3284711 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown but significant domain identity with O-Methyltransferases involved in polyketide biosynthesis; hypothetical protein 3283803..3284711 Mycobacterium ulcerans Agy99 4553436 YP_906695.1 CDS MUL_2962 NC_008611.1 3284701 3285726 R cytoplasmic protein; function unknown, probably involved in cellular metabolism; dehydrogenase complement(3284701..3285726) Mycobacterium ulcerans Agy99 4553437 YP_906696.1 CDS adhC NC_008611.1 3288017 3289030 D cytoplasmic protein; generates aldehyde or ketone from alcohol [catalytic activity: alcohol + NADP(+) = aldehyde or ketone + NADPH]; NADP-dependent alcohol dehydrogenase AdhC 3288017..3289030 Mycobacterium ulcerans Agy99 4553440 YP_906697.1 CDS MUL_2966 NC_008611.1 3289060 3289611 D cytoplasmic protein; function unknown but some similarity with YceI-like family proteins. in E. coli YceI is a base-induced periplasmic protein. its function has not yet been characterised.; hypothetical protein 3289060..3289611 Mycobacterium ulcerans Agy99 4553441 YP_906698.1 CDS MUL_2967 NC_008611.1 3289786 3290457 D membrane protein; hypothetical protein 3289786..3290457 Mycobacterium ulcerans Agy99 4553442 YP_906699.1 CDS MUL_2968 NC_008611.1 3290477 3291634 R cytoplasmic protein; hypothetical protein complement(3290477..3291634) Mycobacterium ulcerans Agy99 4553443 YP_906700.1 CDS MUL_2969 NC_008611.1 3292008 3293054 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3292008..3293054 Mycobacterium ulcerans Agy99 4553444 YP_906701.1 CDS fbpB NC_008611.1 3293710 3294687 D Detected in the membrane fraction by proteomics (LC- MS/MS) Also detected in the cytoplasm by proteomics.; membrane protein; involved in cell wall mycoloylation. proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity.; secreted antigen 85-B FbpB 3293710..3294687 Mycobacterium ulcerans Agy99 4553446 YP_906702.1 CDS MUL_2971 NC_008611.1 3294771 3295445 R cytoplasmic protein; hypothetical protein complement(3294771..3295445) Mycobacterium ulcerans Agy99 4553447 YP_906703.1 CDS MUL_2972 NC_008611.1 3295547 3296491 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 3295547..3296491 Mycobacterium ulcerans Agy99 4553448 YP_906704.1 CDS MUL_2973 NC_008611.1 3296562 3297416 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 3296562..3297416 Mycobacterium ulcerans Agy99 4553449 YP_906705.1 CDS MUL_2974 NC_008611.1 3297433 3298041 D catalyzes the interconversion of chorismate to prephenate; chorismate mutase 3297433..3298041 Mycobacterium ulcerans Agy99 4553450 YP_906706.1 CDS MUL_2975 NC_008611.1 3298133 3298573 D secreted protein; thought to promote the resuscitation and growth of dormant, nongrowing cell. could also stimulate the growth of several other high G+C Gram+ organisms, E.G. mycobacterium avium, mycobacterium bovis (BCG), mycobacterium kansasii, mycobacterium smegmatis.; resuscitation-promoting factor-like protein 3298133..3298573 Mycobacterium ulcerans Agy99 4553569 YP_906707.1 CDS MUL_2976 NC_008611.1 3298629 3299087 D cytoplasmic protein; hypothetical protein 3298629..3299087 Mycobacterium ulcerans Agy99 4553570 YP_906708.1 CDS MUL_2977 NC_008611.1 3299102 3299935 D cytoplasmic protein; function unknown, probably involved in cellular metabolism; short chain dehydrogenase 3299102..3299935 Mycobacterium ulcerans Agy99 4553571 YP_906709.1 CDS lppE NC_008611.1 3299977 3300399 D membrane protein; lipoprotein LppE 3299977..3300399 Mycobacterium ulcerans Agy99 4553572 YP_906710.1 CDS cyp140A5 NC_008611.1 3300510 3301826 D cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.; cytochrome P450 140A5 Cyp140A5 3300510..3301826 Mycobacterium ulcerans Agy99 4553573 YP_906711.1 CDS MUL_2980 NC_008611.1 3301857 3302387 D cytoplasmic protein; hypothetical protein 3301857..3302387 Mycobacterium ulcerans Agy99 4553574 YP_906712.1 CDS MUL_2982 NC_008611.1 3303207 3304253 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3303207..3304253 Mycobacterium ulcerans Agy99 4553576 YP_906713.1 CDS MUL_2983 NC_008611.1 3304324 3305658 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(3304324..3305658) Mycobacterium ulcerans Agy99 4553577 YP_906714.1 CDS MUL_2984 NC_008611.1 3306111 3307157 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3306111..3307157 Mycobacterium ulcerans Agy99 4553578 YP_906715.1 CDS MUL_2988 NC_008611.1 3311428 3312582 R cytoplasmic protein; hypothetical protein complement(3311428..3312582) Mycobacterium ulcerans Agy99 4553580 YP_906716.1 CDS MUL_2992 NC_008611.1 3315364 3315858 R cytoplasmic protein; hypothetical protein complement(3315364..3315858) Mycobacterium ulcerans Agy99 4553583 YP_906717.1 CDS bfrA NC_008611.1 3316300 3316779 R cytoplasmic protein; involved in iron storage (may perform analogous functions in iron detoxification and storage as that of animal ferritins); ferritin is an intracellular molecule that stores iron in a soluble, nontoxic, readily available form. the functional molecule, which is composed of 24 chains, is roughly spherical and contains a central cavity in which the polymeric ferric iron core is deposited.; bacterioferritin BfrA complement(3316300..3316779) Mycobacterium ulcerans Agy99 4553584 YP_906718.1 CDS MUL_2994 NC_008611.1 3317107 3317289 D function unknown but like upstream ORF, contains bacterioferritin-associated ferredoxin domain; hypothetical protein 3317107..3317289 Mycobacterium ulcerans Agy99 4553605 YP_906719.1 CDS echA8_6 NC_008611.1 3317320 3318078 R cytoplasmic protein; oxidizes fatty acids using specific components (by similarity) [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]; enoyl-CoA hydratase, EchA8_6 complement(3317320..3318078) Mycobacterium ulcerans Agy99 4553606 YP_906720.1 CDS MUL_2996 NC_008611.1 3318088 3318531 R Also detected in the membrane fraction by proteomics (LC-MS/MS); cytoplasmic protein; hypothetical protein complement(3318088..3318531) Mycobacterium ulcerans Agy99 4553607 YP_906721.1 CDS MUL_2997 NC_008611.1 3318528 3319241 R cytoplasmic protein; hypothetical protein complement(3318528..3319241) Mycobacterium ulcerans Agy99 4553608 YP_906722.1 CDS oxyR NC_008611.1 3319276 3320220 R cytoplasmic protein; hydrogen peroxide-inducible genes activator, OxyR complement(3319276..3320220) Mycobacterium ulcerans Agy99 4553609 YP_906723.1 CDS ahpC NC_008611.1 3320333 3320920 D Also detected in the extracellular matrix and the membrane fraction by proteomics.; cytoplasmic protein; involved in oxidative stress response.; alkyl hydroperoxide reductase C protein AhpC 3320333..3320920 Mycobacterium ulcerans Agy99 4553610 YP_906724.1 CDS ahpD NC_008611.1 3320961 3321497 D Detected in the cytoplasmic and membrane fractions by LCMSMS and also detected in the extracellular matrix by proteomics.; cytoplasmic protein; involved in oxidative stress response.; alkyl hydroperoxide reductase D protein AhpD 3320961..3321497 Mycobacterium ulcerans Agy99 4553611 YP_906725.1 CDS treS_1 NC_008611.1 3321508 3322860 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in trehalose biosynthesis (protective effect) converts maltose to trehalose. mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)- linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway); trehalose synthase TreS 3321508..3322860 Mycobacterium ulcerans Agy99 4553612 YP_906726.1 CDS lldD2 NC_008611.1 3322976 3324220 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; involved in respiration; catalyzes conversion of lactate into pyruvate [catalytic activity: (S)-lactate + 2 ferricytochrome C = pyruvate + 2 ferrocytochrome C]; L-lactate dehydrogenase (cytochrome) LldD2 3322976..3324220 Mycobacterium ulcerans Agy99 4553613 YP_906727.1 CDS lon NC_008611.1 3324324 3326666 D cytoplasmic protein; function unknown but probably plays a role in posttranslational protein modification, turnover and chaperone functions.; Lon, ATP-dependent Lon protease 3324324..3326666 Mycobacterium ulcerans Agy99 4553614 YP_906728.1 CDS MUL_3004 NC_008611.1 3326771 3327160 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); cytoplasmic protein; hypothetical protein 3326771..3327160 Mycobacterium ulcerans Agy99 4553615 YP_906729.1 CDS MUL_3005 NC_008611.1 3327163 3327495 D cytoplasmic protein; hypothetical protein 3327163..3327495 Mycobacterium ulcerans Agy99 4553616 YP_906730.1 CDS MUL_3006 NC_008611.1 3327619 3328065 D membrane protein; hypothetical protein 3327619..3328065 Mycobacterium ulcerans Agy99 4553617 YP_906731.1 CDS MUL_3007 NC_008611.1 3328167 3330266 R cytoplasmic protein; function unknown but contains nucleoside- diphosphate-sugar epimerase domain identity - a domain associated with cell envelope biogenesis, carbohydrate transport and metabolism.; hypothetical protein complement(3328167..3330266) Mycobacterium ulcerans Agy99 4553618 YP_906732.1 CDS MUL_3008 NC_008611.1 3330388 3331872 R Catalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation; acetyl-CoA acetyltransferase complement(3330388..3331872) Mycobacterium ulcerans Agy99 4553619 YP_906733.1 CDS MUL_3010 NC_008611.1 3334505 3334831 R cytoplasmic protein; function unknown but contains acetyltransferase domain identity; acetyltransferase complement(3334505..3334831) Mycobacterium ulcerans Agy99 4553621 YP_906734.1 CDS MUL_3011 NC_008611.1 3335158 3336015 D cytoplasmic protein; function unknown, probably involved in cellular metabolism; short chain dehydrogenase 3335158..3336015 Mycobacterium ulcerans Agy99 4553622 YP_906735.1 CDS xfp NC_008611.1 3336061 3338463 D Detected in the membrane and secreted fractions by proteomics (2D-LC-MS/MS); membrane protein; bacterial enzyme that splits fructose-6-P and/or xylulose-5-P with the aid of inorganic phosphate into either acetyl-P and erythrose-4-p and/or acetyl-P and glyeraldehyde-3-p; putative phosphoketolase 3336061..3338463 Mycobacterium ulcerans Agy99 4553623 YP_906736.1 CDS MUL_3013 NC_008611.1 3338514 3339179 D cytoplasmic protein; hypothetical protein 3338514..3339179 Mycobacterium ulcerans Agy99 4553640 YP_906737.1 CDS adhA NC_008611.1 3340262 3341290 R cytoplasmic protein; catalyzes the reversible oxidation of ethanol to acetaldehyde with the concomitant reduction of NAD; alcohol dehydrogenase AdhA complement(3340262..3341290) Mycobacterium ulcerans Agy99 4553642 YP_906738.1 CDS MUL_3016 NC_008611.1 3341356 3341643 R membrane protein; hypothetical protein complement(3341356..3341643) Mycobacterium ulcerans Agy99 4553643 YP_906739.1 CDS apa NC_008611.1 3341808 3342728 R Two isoform were also detected by proteomics in the cytoplasm.; membrane protein; function unknown could mediate bacterial attachment to host cells.; alanine and proline rich secreted protein Apa complement(3341808..3342728) Mycobacterium ulcerans Agy99 4553644 YP_906740.1 CDS modC NC_008611.1 3342815 3343936 R membrane protein; part of the binding-protein-dependent transport system ModABC for molybdenum; responsible for energy coupling to the transport system.; molybdenum-transport ABC transporter ATP-binding protein ModC complement(3342815..3343936) Mycobacterium ulcerans Agy99 4553645 YP_906741.1 CDS MUL_5112 NC_008611.1 3344120 3344320 R cytoplasmic protein; hypothetical protein complement(3344120..3344320) Mycobacterium ulcerans Agy99 4553646 YP_906742.1 CDS MUL_3019 NC_008611.1 3344317 3345774 R cytoplasmic protein; function unknown but shows high identity with sulfide-quinone reductase from chlorobium tepidum; hypothetical protein complement(3344317..3345774) Mycobacterium ulcerans Agy99 4553647 YP_906743.1 CDS omt NC_008611.1 3345976 3346809 D cytoplasmic protein; function unknown, but possible involved in lipid metabolism; O-methyltransferase Omt 3345976..3346809 Mycobacterium ulcerans Agy99 4553648 YP_906744.1 CDS modB NC_008611.1 3346821 3347615 R membrane protein; part of the binding-protein-dependent transport system ModABC for molybdenum; responsible for the translocation of the substrate across the membrane.; molybdenum-transport integral membrane protein ABC transporter ModB complement(3346821..3347615) Mycobacterium ulcerans Agy99 4553649 YP_906745.1 CDS modA NC_008611.1 3347624 3348337 R membrane protein; involved in the active transport of molybdenum into the cell across the membrane (import) part of the binding- protein-dependent transport system ModABC.; molybdate-binding lipoprotein ModA complement(3347624..3348337) Mycobacterium ulcerans Agy99 4553650 YP_906746.1 CDS MUL_3023 NC_008611.1 3348675 3349352 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; short chain dehydrogenase 3348675..3349352 Mycobacterium ulcerans Agy99 4553651 YP_906747.1 CDS MUL_3024 NC_008611.1 3349403 3350326 D cytoplasmic protein; function unknown, probably involved in cellular metabolism; oxidoreductase 3349403..3350326 Mycobacterium ulcerans Agy99 4553652 YP_906748.1 CDS ndh NC_008611.1 3350490 3351875 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; transfer of electrons from NADH to the respiratory chain. the immediate electron acceptor for the enzyme is believed to be ubiquinone. does not couple the redox reaction to proton translocation.; NADH dehydrogenase Ndh 3350490..3351875 Mycobacterium ulcerans Agy99 4553653 YP_906749.1 CDS ureD NC_008611.1 3351946 3352590 R cytoplasmic protein; UreD is involved in activation of the urease enzyme via the UreD-UreF-UreG-urease complex and is required for urease nickel metallocenter assembly; urease accessory protein UreD complement(3351946..3352590) Mycobacterium ulcerans Agy99 4553654 YP_906750.1 CDS ureG NC_008611.1 3352590 3353261 R cytoplasmic protein; UreG is required for functional incorporation of the urease nickel metallocenter. it is not known whether UreG binds GTP or some other nucleotide.; urease accessory protein UreG complement(3352590..3353261) Mycobacterium ulcerans Agy99 4553655 YP_906751.1 CDS ureF NC_008611.1 3353272 3353892 R cytoplasmic protein; required for the insertion of the nickel ion at the active site of the urease protein; urease accessory protein UreF complement(3353272..3353892) Mycobacterium ulcerans Agy99 4553656 YP_906752.1 CDS ureC NC_008611.1 3353907 3355640 R ureases catalyze the hydrolysis of urea into ammonia and carbon dioxide; in Helicobacter pylori the ammonia released plays a key role in bacterial survival by neutralizing acids when colonizing the gastric mucosa; the holoenzyme is composed of 3 ureC (alpha) and 3 ureAB (gamma/beta) subunits; urease subunit alpha complement(3353907..3355640) Mycobacterium ulcerans Agy99 4553657 YP_906753.1 CDS ureB NC_008611.1 3355640 3355951 R cytoplasmic protein; involved in the conversion of UreA to NH3 [catalytic activity: UreA + H2O = CO2 + 2 NH3]; urease subunit beta UreB complement(3355640..3355951) Mycobacterium ulcerans Agy99 4553658 YP_906754.1 CDS ureA NC_008611.1 3355948 3356250 R UreA, with UreB and UreC catalyzes the hydrolysis of urea into ammonia and carbon dioxide; nickel metalloenzyme; accessory proteins UreD, UreE, UreF, and UreG are necessary for assembly of the metallocenter; urease subunit gamma complement(3355948..3356250) Mycobacterium ulcerans Agy99 4553659 YP_906755.1 CDS MUL_3032 NC_008611.1 3356310 3356750 R cytoplasmic protein; function unknown but domain identity suggests possibly involved in aromatic compound catabolism; hypothetical protein complement(3356310..3356750) Mycobacterium ulcerans Agy99 4553660 YP_906756.1 CDS MUL_3033 NC_008611.1 3357177 3357593 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein 3357177..3357593 Mycobacterium ulcerans Agy99 4553661 YP_906757.1 CDS MUL_3034 NC_008611.1 3357608 3358558 D membrane protein; function unknown but significant domain identity with HtpX, Zn-dependent protease with chaperone function.; hypothetical protein 3357608..3358558 Mycobacterium ulcerans Agy99 4553662 YP_906758.1 CDS gnd1 NC_008611.1 3358668 3360119 D catalyzes the formation of D-ribulose 5-phosphate from 6-phospho-D-gluconate; 6-phosphogluconate dehydrogenase 3358668..3360119 Mycobacterium ulcerans Agy99 4553663 YP_906759.1 CDS guaB1 NC_008611.1 3360151 3361587 D catalyzes the synthesis of xanthosine monophosphate by the NAD+ dependent oxidation of inosine monophosphate; inosine 5-monophosphate dehydrogenase 3360151..3361587 Mycobacterium ulcerans Agy99 4553664 YP_906760.1 CDS ilvG NC_008611.1 3363463 3365115 D cytoplasmic protein; valine and isoleucine biosynthesis (first step) [catalytic activity : 2-acetolactate + CO(2) = 2 pyruvate]; hypothetical protein 3363463..3365115 Mycobacterium ulcerans Agy99 4553667 YP_906761.1 CDS secA2 NC_008611.1 3365146 3367566 D SecA2; functions in protein export; can interact with acidic membrane phospholipids and the SecYEG protein complex; binds to preproteins; binds to ATP and undergoes a conformational change to promote membrane insertion of SecA/bound preprotein; ATP hydrolysis appears to drive release of the preprotein from SecA and deinsertion of SecA from the membrane; additional proteins SecD/F/YajC aid SecA recycling; exists in an equilibrium between monomers and dimers; may possibly form higher order oligomers; proteins in this cluster correspond to SecA2; which is non-essential and seems to play a role in secretion of a subset of proteins; preprotein translocase subunit SecA 3365146..3367566 Mycobacterium ulcerans Agy99 4553668 YP_906762.1 CDS pgsA2 NC_008611.1 3367687 3368316 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; thought to be involved in cardiolipin biosynthesis; generates cardiolipin from phosphatidylglycerol and CDP- diacylglycerol [catalytic activity : may be: phosphatidylglycerol + phosphatidylglycerol -> cardiolipin + glycerol, or: CDP-diacylglycerol + glycerol 3-phosphate = CMP + 3-(3-phosphatidyl)-glycerol 1-phosphate]; CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase 3367687..3368316 Mycobacterium ulcerans Agy99 4553669 YP_906763.1 CDS MUL_3042 NC_008611.1 3368309 3369229 D cytoplasmic protein; hypothetical protein 3368309..3369229 Mycobacterium ulcerans Agy99 4553670 YP_906764.1 CDS MUL_3043 NC_008611.1 3369255 3369623 D membrane protein; hypothetical protein 3369255..3369623 Mycobacterium ulcerans Agy99 4553671 YP_906765.1 CDS MUL_3044 NC_008611.1 3369648 3370538 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 3369648..3370538 Mycobacterium ulcerans Agy99 4553672 YP_906766.1 CDS gcvH NC_008611.1 3370573 3370977 D part of multienzyme complex composed of H, L, P, and T proteins which catalyzes oxidation of glycine to yield carbon dioxide, ammonia, 5,10-CH2-H4folate and a reduced pyridine nucleotide; protein H is involved in transfer of methylamine group from the P to T protein; covalently bound to a lipoyl cofactor; glycine cleavage system protein H 3370573..3370977 Mycobacterium ulcerans Agy99 4553673 YP_906767.1 CDS MUL_3046 NC_008611.1 3371235 3371723 D Detected in the cytoplamic fraction by LC-MS/MS; cytoplasmic protein; function unknown but contains an FHA domain. these domains are involved in nuclear signalling domain and may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine.; hypothetical protein 3371235..3371723 Mycobacterium ulcerans Agy99 4553674 YP_906768.1 CDS MUL_3047 NC_008611.1 3371720 3372475 D cytoplasmic protein; function unknown but high N-terminal domain identity with the MerR helix-turn-helix transcription regulator.; putative regulatory protein 3371720..3372475 Mycobacterium ulcerans Agy99 4553675 YP_906769.1 CDS MUL_3048 NC_008611.1 3372616 3373110 D cytoplasmic protein; hypothetical protein 3372616..3373110 Mycobacterium ulcerans Agy99 4553676 YP_906770.1 CDS MUL_3049 NC_008611.1 3373388 3374020 D cytoplasmic protein; function unknown but contains MerR helix-turn-helix transcription regulatory domain; regulatory protein 3373388..3374020 Mycobacterium ulcerans Agy99 4553677 YP_906771.1 CDS gcvB NC_008611.1 3374383 3377220 D acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine; glycine dehydrogenase 3374383..3377220 Mycobacterium ulcerans Agy99 4553678 YP_906772.1 CDS MUL_3052 NC_008611.1 3378726 3379598 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; may act on a wide range of 1-haloalkanes, haloalcohols, haloalkenes and some haloaromatic compounds [catalytic activity: 1-haloalkane + H(2)O, a primary alcohol + halide]; haloalkane dehalogenase complement(3378726..3379598) Mycobacterium ulcerans Agy99 4553680 YP_906773.1 CDS MUL_3053 NC_008611.1 3379582 3380514 D cytoplasmic protein; hydrolase 3379582..3380514 Mycobacterium ulcerans Agy99 4553681 YP_906774.1 CDS MUL_3054 NC_008611.1 3380544 3382751 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; function unknown but weak C-term identity with vWA domain (von willebrand factor type a domain) this domain is found in extracellular proteins, like integrins, and mediates adhesion.; hypothetical protein complement(3380544..3382751) Mycobacterium ulcerans Agy99 4553682 YP_906775.1 CDS glcB NC_008611.1 3382802 3384997 R catalyzes the formation of malate from glyoxylate and acetyl-CoA; malate synthase G complement(3382802..3384997) Mycobacterium ulcerans Agy99 4553683 YP_906776.1 CDS MUL_3056 NC_008611.1 3385216 3386814 R PE_PGRS34; membrane protein; PE-PGRS family protein complement(3385216..3386814) Mycobacterium ulcerans Agy99 4553684 YP_906777.1 CDS MUL_3059 NC_008611.1 3389118 3391037 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; thought to be involved in active transport of drugs across the membrane (export): multidrugs resistance by an export mechanism. responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane.; drug-transport transmembrane ABC transporter ATP-binding protein 3389118..3391037 Mycobacterium ulcerans Agy99 4553687 YP_906778.1 CDS MUL_3060 NC_008611.1 3391292 3392338 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(3391292..3392338) Mycobacterium ulcerans Agy99 4553688 YP_906779.1 CDS MUL_3061 NC_008611.1 3392966 3394300 D cytoplasmic protein; required for the transposition of the insertion element IS2606; transposase for IS2606 3392966..3394300 Mycobacterium ulcerans Agy99 4553689 YP_906780.1 CDS MUL_3062 NC_008611.1 3394387 3396999 D PE_PGRS9_1; membrane protein; PE-PGRS family protein 3394387..3396999 Mycobacterium ulcerans Agy99 4553690 YP_906781.1 CDS MUL_3063 NC_008611.1 3397045 3399021 R translation fusion of two CDS caused by frameshift at 3' end of hypothetical protein MON6433 (3398526); membrane protein; function unknown, probably involved in cellular metabolism; flavoprotein complement(3397045..3399021) Mycobacterium ulcerans Agy99 4553691 YP_906782.1 CDS MUL_3065 NC_008611.1 3399900 3400946 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3399900..3400946 Mycobacterium ulcerans Agy99 4553693 YP_906783.1 CDS erg3 NC_008611.1 3401367 3402329 R membrane protein; involved in lipid desaturation; membrane-bound C-5 sterol desaturase Erg3 complement(3401367..3402329) Mycobacterium ulcerans Agy99 4553694 YP_906784.1 CDS MUL_3067 NC_008611.1 3402593 3403123 D cytoplasmic protein; function unknown - weak domain identity with lipase/esterase proteins; hypothetical protein 3402593..3403123 Mycobacterium ulcerans Agy99 4553695 YP_906785.1 CDS MUL_3068 NC_008611.1 3403120 3404319 D cytoplasmic protein; function unknown, probably involved in cellular metabolism; NADH dehydrogenase 3403120..3404319 Mycobacterium ulcerans Agy99 4553696 YP_906786.1 CDS mgtC NC_008611.1 3404321 3405049 R membrane protein; thought to be involved in Mg2+ transport (import) may act as an accessory protein for MgtB so mediating magnesium influx into the cytosol [catalytic activity: ATP + H(2)O + mg(2+)(out) = ADP + phosphate + mg(2+)(in)]; Mg2+ transport p-type ATPase C MgtC complement(3404321..3405049) Mycobacterium ulcerans Agy99 4553697 YP_906787.1 CDS MUL_3070 NC_008611.1 3405257 3405493 R cytoplasmic protein; hypothetical protein complement(3405257..3405493) Mycobacterium ulcerans Agy99 4553698 YP_906788.1 CDS MUL_3071 NC_008611.1 3405897 3407303 R PPE30; membrane protein; PPE family protein complement(3405897..3407303) Mycobacterium ulcerans Agy99 4553699 YP_906789.1 CDS MUL_3072 NC_008611.1 3407390 3408670 R PPE32; membrane protein; PPE family protein complement(3407390..3408670) Mycobacterium ulcerans Agy99 4553700 YP_906790.1 CDS MUL_3074 NC_008611.1 3409755 3410915 R PPE31; membrane protein; PPE family protein complement(3409755..3410915) Mycobacterium ulcerans Agy99 4553701 YP_906791.1 CDS MUL_3076 NC_008611.1 3412998 3414350 R PPE31_1; membrane protein; PPE family protein complement(3412998..3414350) Mycobacterium ulcerans Agy99 4553703 YP_906792.1 CDS MUL_3077 NC_008611.1 3415250 3417082 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (LC-MS/MS); membrane protein; function unknown, but domain identity to aaa, ATPase family proteins that have been associated with chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes.; hypothetical protein complement(3415250..3417082) Mycobacterium ulcerans Agy99 4553704 YP_906793.1 CDS MUL_3078 NC_008611.1 3417079 3418290 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(3417079..3418290) Mycobacterium ulcerans Agy99 4553705 YP_906794.1 CDS mycP5 NC_008611.1 3418287 3420026 R Also detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; function unknown proteolytic activity but subtilases are a family of serine proteases that can play a role in posttranslational modification.; proline rich membrane-anchored mycosin MycP5 complement(3418287..3420026) Mycobacterium ulcerans Agy99 4553706 YP_906795.1 CDS MUL_3080 NC_008611.1 3420004 3421515 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; hypothetical protein complement(3420004..3421515) Mycobacterium ulcerans Agy99 4553707 YP_906796.1 CDS MUL_3081 NC_008611.1 3421800 3422702 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); cytoplasmic protein; hypothetical protein complement(3421800..3422702) Mycobacterium ulcerans Agy99 4553708 YP_906797.1 CDS esxN NC_008611.1 3422791 3423075 R Also Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; secreted protein; EsaT-6 like protein EsxN complement(3422791..3423075) Mycobacterium ulcerans Agy99 4553709 YP_906798.1 CDS esxM NC_008611.1 3423127 3423429 R Detected in the secreted fraction by proteomics.; secreted protein; EsaT-6 like protein EsxM complement(3423127..3423429) Mycobacterium ulcerans Agy99 4553710 YP_906799.1 CDS MUL_3084 NC_008611.1 3423773 3424072 R PE19; membrane protein; PE family protein complement(3423773..3424072) Mycobacterium ulcerans Agy99 4553711 YP_906800.1 CDS MUL_3085 NC_008611.1 3424894 3425940 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3424894..3425940 Mycobacterium ulcerans Agy99 4553712 YP_906801.1 CDS MUL_3086 NC_008611.1 3426028 3426348 R secreted protein; hypothetical protein complement(3426028..3426348) Mycobacterium ulcerans Agy99 4553731 YP_906802.1 CDS MUL_3087 NC_008611.1 3426422 3427642 R PPE26; Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; PPE family protein complement(3426422..3427642) Mycobacterium ulcerans Agy99 4553732 YP_906803.1 CDS MUL_3088 NC_008611.1 3427676 3427975 R PE19_1; membrane protein; PE family protein complement(3427676..3427975) Mycobacterium ulcerans Agy99 4553733 YP_906804.1 CDS MUL_3089 NC_008611.1 3428174 3429430 R PPE25; membrane protein; PPE family protein complement(3428174..3429430) Mycobacterium ulcerans Agy99 4553734 YP_906805.1 CDS MUL_3090 NC_008611.1 3429982 3430176 R cytoplasmic protein; ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.; ferredoxin complement(3429982..3430176) Mycobacterium ulcerans Agy99 4553736 YP_906806.1 CDS cyp143A4 NC_008611.1 3430439 3431614 D cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.; cytochrome P450 143A4 Cyp143A4 3430439..3431614 Mycobacterium ulcerans Agy99 4553737 YP_906807.1 CDS MUL_3092 NC_008611.1 3431646 3435818 R Detected in the membrane fraction by proteomics.; membrane protein; function unknown but has multiple FtsK/SpoIIIE protein domains. This domain contains a putative ATP binding P-loop motif and is found in the FtsK cell division protein from E. coli and the stage III sporulation protein E SpoIIIE.; FtsK/SpoIIIE family protein complement(3431646..3435818) Mycobacterium ulcerans Agy99 4553738 YP_906808.1 CDS MUL_3093 NC_008611.1 3435815 3437338 R Detected in the membrane fraction by proteomics.; membrane protein; hypothetical protein complement(3435815..3437338) Mycobacterium ulcerans Agy99 4553739 YP_906809.1 CDS malQ NC_008611.1 3437662 3439833 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; transfers a segment of a (1,4)-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or (1,4)-alpha-D-glucan; 4-alpha-glucanotransferase 3437662..3439833 Mycobacterium ulcerans Agy99 4553740 YP_906810.1 CDS MUL_3096 NC_008611.1 3440536 3441099 R cytoplasmic protein; hypothetical protein complement(3440536..3441099) Mycobacterium ulcerans Agy99 4553742 YP_906811.1 CDS MUL_3098 NC_008611.1 3443396 3443827 D cytoplasmic protein; hypothetical protein 3443396..3443827 Mycobacterium ulcerans Agy99 4553744 YP_906812.1 CDS MUL_3101 NC_008611.1 3447693 3448307 D cytoplasmic protein; hypothetical protein 3447693..3448307 Mycobacterium ulcerans Agy99 4553747 YP_906813.1 CDS MUL_3103 NC_008611.1 3450497 3451024 D cytoplasmic protein; hypothetical protein 3450497..3451024 Mycobacterium ulcerans Agy99 4553803 YP_906814.1 CDS MUL_3104 NC_008611.1 3451706 3452794 R cytoplasmic protein; glycosyl transferase family protein complement(3451706..3452794) Mycobacterium ulcerans Agy99 4553804 YP_906815.1 CDS MUL_3105 NC_008611.1 3452897 3453766 R membrane protein; function unknown but contains signal peptide; hypothetical protein complement(3452897..3453766) Mycobacterium ulcerans Agy99 4553805 YP_906816.1 CDS cyp143A3 NC_008611.1 3453819 3454991 R cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.; cytochrome P450 143A3 Cyp143A3 complement(3453819..3454991) Mycobacterium ulcerans Agy99 4553806 YP_906817.1 CDS MUL_3107 NC_008611.1 3455153 3455773 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown - contains signal peptide; hypothetical protein complement(3455153..3455773) Mycobacterium ulcerans Agy99 4553807 YP_906818.1 CDS MUL_3108 NC_008611.1 3456003 3456896 D cytoplasmic protein; short-chain type dehydrogenase/reductase 3456003..3456896 Mycobacterium ulcerans Agy99 4553808 YP_906819.1 CDS MUL_3110 NC_008611.1 3458892 3459548 D cytoplasmic protein; hypothetical protein 3458892..3459548 Mycobacterium ulcerans Agy99 4553810 YP_906820.1 CDS MUL_3111 NC_008611.1 3459548 3459931 D cytoplasmic protein; hypothetical protein 3459548..3459931 Mycobacterium ulcerans Agy99 4553811 YP_906821.1 CDS MUL_3113 NC_008611.1 3460717 3461355 D cytoplasmic protein; involved in transcriptional mechanism.; AcrR family transcriptional regulator 3460717..3461355 Mycobacterium ulcerans Agy99 4553813 YP_906822.1 CDS MUL_3114 NC_008611.1 3461469 3461960 R cytoplasmic protein; hypothetical protein complement(3461469..3461960) Mycobacterium ulcerans Agy99 4553814 YP_906823.1 CDS MUL_3115 NC_008611.1 3462327 3462881 D cytoplasmic protein; could cause methylation of unknown substrate.; methyltransferase 3462327..3462881 Mycobacterium ulcerans Agy99 4553815 YP_906824.1 CDS trxB1_1 NC_008611.1 3463025 3463321 R cytoplasmic protein; thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions.; thioredoxin TrxB1 complement(3463025..3463321) Mycobacterium ulcerans Agy99 4553816 YP_906825.1 CDS MUL_3117 NC_008611.1 3463457 3463726 D cytoplasmic protein; hypothetical protein 3463457..3463726 Mycobacterium ulcerans Agy99 4553817 YP_906826.1 CDS MUL_3118 NC_008611.1 3463760 3464185 D cytoplasmic protein; hypothetical protein 3463760..3464185 Mycobacterium ulcerans Agy99 4553818 YP_906827.1 CDS MUL_3119 NC_008611.1 3464241 3464603 D cytoplasmic protein; hypothetical protein 3464241..3464603 Mycobacterium ulcerans Agy99 4553819 YP_906828.1 CDS MUL_3122 NC_008611.1 3466651 3467892 D hypothetical protein 3466651..3467892 Mycobacterium ulcerans Agy99 4553821 YP_906829.1 CDS MUL_3123 NC_008611.1 3467960 3469165 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown but contains aminopeptidase domain identity; hypothetical protein 3467960..3469165 Mycobacterium ulcerans Agy99 4553822 YP_906830.1 CDS MUL_3124 NC_008611.1 3469162 3470448 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown, probably involved in cellular metabolism; oxidoreductase 3469162..3470448 Mycobacterium ulcerans Agy99 4553841 YP_906831.1 CDS MUL_3125 NC_008611.1 3470542 3471921 D cytoplasmic protein; hypothetical protein 3470542..3471921 Mycobacterium ulcerans Agy99 4553842 YP_906832.1 CDS MUL_3127 NC_008611.1 3472767 3474053 D membrane protein; PPE family protein 3472767..3474053 Mycobacterium ulcerans Agy99 4553843 YP_906833.1 CDS MUL_3128 NC_008611.1 3474405 3474959 R cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein complement(3474405..3474959) Mycobacterium ulcerans Agy99 4553844 YP_906834.1 CDS cyp144A4 NC_008611.1 3479506 3480720 D cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.; cytochrome P450 144A4 Cyp144A4 3479506..3480720 Mycobacterium ulcerans Agy99 4553846 YP_906835.1 CDS MUL_3133 NC_008611.1 3481883 3482929 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(3481883..3482929) Mycobacterium ulcerans Agy99 4553848 YP_906836.1 CDS MUL_3138 NC_008611.1 3486073 3486750 R secreted protein; hypothetical protein complement(3486073..3486750) Mycobacterium ulcerans Agy99 4553851 YP_906837.1 CDS MUL_3139 NC_008611.1 3486810 3487607 R Also detected in the membrane fraction by proteomics (LC-MS/MS); cytoplasmic protein; function unknown, probably involved in cellular metabolism; short-chain dehydrogenase complement(3486810..3487607) Mycobacterium ulcerans Agy99 4553852 YP_906838.1 CDS MUL_3141 NC_008611.1 3490098 3490820 R function unknown, lipolytic enzyme probably involved in cellular metabolism.; esterase/lipase complement(3490098..3490820) Mycobacterium ulcerans Agy99 4553854 YP_906839.1 CDS MUL_3142 NC_008611.1 3490955 3491371 D cytoplasmic protein; function unknown but domain identity suggests it may be a signal-transduction protein; hypothetical protein 3490955..3491371 Mycobacterium ulcerans Agy99 4553855 YP_906840.1 CDS MUL_3143 NC_008611.1 3491430 3492620 R cytoplasmic protein; function unknown role in cellular metabolism; amidohydrolase complement(3491430..3492620) Mycobacterium ulcerans Agy99 4553856 YP_906841.1 CDS araD NC_008611.1 3492746 3493570 R cytoplasmic protein; domain identity suggests this CDS encodes an AraD- like, ribulose-5-phosphate 4-epimerase. this enzyme and related epimerases and aldolases are involved in carbohydrate transport and metabolism.; ribulose-5-phosphate 4-epimerase, AraD complement(3492746..3493570) Mycobacterium ulcerans Agy99 4553857 YP_906842.1 CDS MUL_3145 NC_008611.1 3493506 3494918 R cytoplasmic protein; function unknown but domain identity suggests this CDS is Acs-like, acyl-coenzyme a synthetases/AMP-(fatty) acid ligases involved in lipid metabolism.; hypothetical protein complement(3493506..3494918) Mycobacterium ulcerans Agy99 4553858 YP_906843.1 CDS MUL_3146 NC_008611.1 3495483 3495905 D cytoplasmic protein; acetyltransferase 3495483..3495905 Mycobacterium ulcerans Agy99 4553859 YP_906844.1 CDS MUL_3147 NC_008611.1 3496050 3496991 D membrane protein; function unknown but some domain identity with Zn- dependent proteases; hypothetical protein 3496050..3496991 Mycobacterium ulcerans Agy99 4553860 YP_906845.1 CDS MUL_3148 NC_008611.1 3497062 3497577 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 3497062..3497577 Mycobacterium ulcerans Agy99 4553861 YP_906846.1 CDS MUL_3149 NC_008611.1 3497610 3500264 R Detected in the membrane fraction by proteomics (LC- MS/MS) Also detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; thought to be involved in active transport of undeterminated substrate (possibly lipooligosaccharide) across the membrane. responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane.; transmembrane ABC transporter ATP-binding protein complement(3497610..3500264) Mycobacterium ulcerans Agy99 4553862 YP_906847.1 CDS MUL_3150 NC_008611.1 3500384 3501718 D cytoplasmic protein; hypothetical protein 3500384..3501718 Mycobacterium ulcerans Agy99 4553863 YP_906848.1 CDS MUL_3152 NC_008611.1 3503183 3504229 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3503183..3504229 Mycobacterium ulcerans Agy99 4553865 YP_906849.1 CDS MUL_3153 NC_008611.1 3504298 3505632 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(3504298..3505632) Mycobacterium ulcerans Agy99 4553866 YP_906850.1 CDS MUL_3154 NC_008611.1 3506887 3507744 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; dehydrogenase complement(3506887..3507744) Mycobacterium ulcerans Agy99 4553867 YP_906851.1 CDS MUL_3155 NC_008611.1 3508023 3509330 R membrane protein; involved in transport of unknown substrate across the membrane.; hypothetical protein complement(3508023..3509330) Mycobacterium ulcerans Agy99 4553868 YP_906852.1 CDS MUL_3156 NC_008611.1 3509487 3509828 R cytoplasmic protein; hypothetical protein complement(3509487..3509828) Mycobacterium ulcerans Agy99 4553869 YP_906853.1 CDS MUL_3157 NC_008611.1 3509874 3510341 R cytoplasmic protein; hypothetical protein complement(3509874..3510341) Mycobacterium ulcerans Agy99 4553870 YP_906854.1 CDS MUL_3160 NC_008611.1 3511854 3512702 D Detected in the cytoplamic and membrane fractions by LCMSMS; cytoplasmic protein; possibly involved in detoxification reactions following oxidative damage to lipids.; short-chain dehydrogenase 3511854..3512702 Mycobacterium ulcerans Agy99 4553872 YP_906855.1 CDS MUL_3161 NC_008611.1 3512788 3514137 D cytoplasmic protein; hypothetical protein 3512788..3514137 Mycobacterium ulcerans Agy99 4553873 YP_906856.1 CDS MUL_3162 NC_008611.1 3514369 3515529 D cytoplasmic protein; hypothetical protein 3514369..3515529 Mycobacterium ulcerans Agy99 4553874 YP_906857.1 CDS MUL_3166 NC_008611.1 3518971 3519729 D Detected in the secreted protein fractions by 2D-LC- MS/MS.; secreted protein; hypothetical protein 3518971..3519729 Mycobacterium ulcerans Agy99 4553878 YP_906858.1 CDS MUL_3167 NC_008611.1 3519745 3521040 R membrane protein; H(+)-stimulated, highly selective, divalent cation uptake system. responsible for the translocation of the divalent metal across the membrane.; divalent cation-transport integral membrane protein complement(3519745..3521040) Mycobacterium ulcerans Agy99 4553879 YP_906859.1 CDS MUL_3168 NC_008611.1 3521254 3522048 D cytoplasmic protein; short chain dehydrogenase 3521254..3522048 Mycobacterium ulcerans Agy99 4553880 YP_906860.1 CDS MUL_3169 NC_008611.1 3522069 3526601 R PPE8; membrane protein; PPE family protein complement(3522069..3526601) Mycobacterium ulcerans Agy99 4553881 YP_906861.1 CDS MUL_3170 NC_008611.1 3527230 3528096 D cytoplasmic protein; hypothetical protein 3527230..3528096 Mycobacterium ulcerans Agy99 4553882 YP_906862.1 CDS MUL_3171 NC_008611.1 3528579 3529271 D cytoplasmic protein; involved in the synthesis of decaprenyl diphosphate, a molecule which has a central role in the biosynthesis of most features of the mycobacterial cell wall. adds seven more isoprene units to omega,E, Z- farnesyl diphosphate and releases decaprenyl diphosphate.; Z-decaprenyl diphosphate synthase 3528579..3529271 Mycobacterium ulcerans Agy99 4553883 YP_906863.1 CDS MUL_3172 NC_008611.1 3529484 3530422 D membrane protein; hypothetical protein 3529484..3530422 Mycobacterium ulcerans Agy99 4553884 YP_906864.1 CDS ndh1 NC_008611.1 3530419 3531543 D cytoplasmic protein; transfer of electrons from NADH to the respiratory chain. the immediate electron acceptor for the enzyme is believed to be ubiquinone. does not couple the redox reaction to proton translocation.; NADH dehydrogenase Ndh1 3530419..3531543 Mycobacterium ulcerans Agy99 4553885 YP_906865.1 CDS MUL_3174 NC_008611.1 3531552 3533021 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); secreted protein; function unknown but contains signal peptide; hypothetical protein complement(3531552..3533021) Mycobacterium ulcerans Agy99 4553886 YP_906866.1 CDS tex NC_008611.1 3533237 3535600 R cytoplasmic protein; function unknown but may play a role in protein translation; transcriptional accessory protein Tex complement(3533237..3535600) Mycobacterium ulcerans Agy99 4553887 YP_906867.1 CDS ceoC1 NC_008611.1 3535670 3536392 R membrane protein; part of a potassium transport system.; TRK system potassium uptake protein CeoC1 complement(3535670..3536392) Mycobacterium ulcerans Agy99 4553888 YP_906868.1 CDS MUL_3177 NC_008611.1 3536639 3538135 D the Vibrio parahaemolyticus gene VP2867 was found to be a potassium/proton antiporter; can rapidly extrude potassium against a potassium gradient at alkaline pH when cloned and expressed in Escherichia coli; potassium/proton antiporter 3536639..3538135 Mycobacterium ulcerans Agy99 4553889 YP_906869.1 CDS MUL_3178 NC_008611.1 3538145 3539500 R membrane protein; hypothetical protein complement(3538145..3539500) Mycobacterium ulcerans Agy99 4553890 YP_906870.1 CDS MUL_3179 NC_008611.1 3539683 3540729 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(3539683..3540729) Mycobacterium ulcerans Agy99 4553891 YP_906871.1 CDS pknE NC_008611.1 3541009 3542772 R Detected in the membrane fraction by proteomics.; membrane protein; involved in signal transduction (via phosphorylation) thought to be involved in membrane transport [catalytic activity: ATP + a protein = ADP + a phosphoprotein]; Ser/Thr protein kinase complement(3541009..3542772) Mycobacterium ulcerans Agy99 4553892 YP_906872.1 CDS MUL_3181 NC_008611.1 3543001 3543738 R cytoplasmic protein; hypothetical protein complement(3543001..3543738) Mycobacterium ulcerans Agy99 4554108 YP_906873.1 CDS potE NC_008611.1 3543831 3545090 R membrane protein; thought to be involved in transport amino acids across the membrane.; amino acid transporter PotE complement(3543831..3545090) Mycobacterium ulcerans Agy99 4554109 YP_906874.1 CDS MUL_3184 NC_008611.1 3547996 3548367 D cytoplasmic protein; hypothetical protein 3547996..3548367 Mycobacterium ulcerans Agy99 4554111 YP_906875.1 CDS MUL_3186 NC_008611.1 3551848 3553269 R membrane protein; function unknown. seems to influence both cell surface interactions among mycobacteria and the interactions of bacteria with macrophages.; PE-PGRS family protein complement(3551848..3553269) Mycobacterium ulcerans Agy99 4554113 YP_906876.1 CDS MUL_3187 NC_008611.1 3553400 3554821 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in active transport of unknown sugars across the membrane. responsible for the translocation of the substrate across the membrane.; sugar transporter complement(3553400..3554821) Mycobacterium ulcerans Agy99 4554114 YP_906877.1 CDS MUL_3188 NC_008611.1 3555174 3556034 D hypothetical protein 3555174..3556034 Mycobacterium ulcerans Agy99 4554115 YP_906878.1 CDS MUL_3189 NC_008611.1 3556336 3557523 D Detected in the membrane fraction by proteomics (LC- MS/MS); secreted protein; function unknown but contains signal peptide and domain identity with periplasmic component of the tol biopolymer transport system.; hypothetical protein 3556336..3557523 Mycobacterium ulcerans Agy99 4554116 YP_906879.1 CDS gabD2 NC_008611.1 3557527 3559083 R NADP-dependent semialdehyde dehydrogenase; part of alternative pathway from alpha-ketoglutarate to succinate; succinate-semialdehyde dehydrogenase complement(3557527..3559083) Mycobacterium ulcerans Agy99 4554117 YP_906880.1 CDS MUL_3191 NC_008611.1 3559252 3560238 R cytoplasmic protein; function unknown but domain identity with penicillin V acylase and related amidases; hydrolase/amidase complement(3559252..3560238) Mycobacterium ulcerans Agy99 4554118 YP_906881.1 CDS MUL_3192 NC_008611.1 3560491 3561168 D secreted protein; function unknown but contains signal peptide; hypothetical protein 3560491..3561168 Mycobacterium ulcerans Agy99 4554119 YP_906882.1 CDS MUL_3194 NC_008611.1 3564025 3564333 R membrane protein; hypothetical protein complement(3564025..3564333) Mycobacterium ulcerans Agy99 4554121 YP_906883.1 CDS MUL_3195 NC_008611.1 3564363 3565391 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; hydrolase 3564363..3565391 Mycobacterium ulcerans Agy99 4554122 YP_906884.1 CDS MUL_3196 NC_008611.1 3565433 3566731 R cytoplasmic protein; hypothetical protein complement(3565433..3566731) Mycobacterium ulcerans Agy99 4554123 YP_906885.1 CDS idsB NC_008611.1 3566788 3567774 R cytoplasmic protein; involved in biosynthesis of membrane ether-linked lipids. catalyzes the trans-addition of the three molecules of Ipp onto DMAPP to form geranylgeranyl pyrophosphate which is a precursor of the ether-linked lipids. catalyzes the consecutive condensation of; polyprenyl synthetase IdsB complement(3566788..3567774) Mycobacterium ulcerans Agy99 4554124 YP_906886.1 CDS MUL_3198 NC_008611.1 3568205 3568714 D cytoplasmic protein; function unknown but may be involved in inhibition of protein synthesis by cleavage of mRNA; translation initiation inhibitor 3568205..3568714 Mycobacterium ulcerans Agy99 4554125 YP_906887.1 CDS MUL_3199 NC_008611.1 3568769 3569710 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; hypothetical protein 3568769..3569710 Mycobacterium ulcerans Agy99 4554126 YP_906888.1 CDS MUL_3200 NC_008611.1 3569700 3570026 R cytoplasmic protein; hypothetical protein complement(3569700..3570026) Mycobacterium ulcerans Agy99 4554127 YP_906889.1 CDS MUL_3201 NC_008611.1 3570202 3571014 D cytoplasmic protein; involved in transcriptional mechanism.; GntR family transcriptional regulator 3570202..3571014 Mycobacterium ulcerans Agy99 4554158 YP_906890.1 CDS MUL_3202 NC_008611.1 3571023 3572045 D cytoplasmic protein; oxidoreductase 3571023..3572045 Mycobacterium ulcerans Agy99 4554159 YP_906891.1 CDS MUL_3203 NC_008611.1 3572106 3572912 D cytoplasmic protein; hypothetical protein 3572106..3572912 Mycobacterium ulcerans Agy99 4554160 YP_906892.1 CDS MUL_3204 NC_008611.1 3572873 3573919 R Detected in the membrane fraction by proteomics; cytoplasmic protein; function unknown role in carbohydrate transport and metabolism; aldose 1-epimerase complement(3572873..3573919) Mycobacterium ulcerans Agy99 4554161 YP_906893.1 CDS MUL_5114 NC_008611.1 3574204 3574650 D cytoplasmic protein; hypothetical protein 3574204..3574650 Mycobacterium ulcerans Agy99 4554162 YP_906894.1 CDS MUL_3206 NC_008611.1 3575202 3575537 D secreted protein; hypothetical protein 3575202..3575537 Mycobacterium ulcerans Agy99 4554164 YP_906895.1 CDS MUL_3207 NC_008611.1 3575588 3575866 D secreted protein; hypothetical protein 3575588..3575866 Mycobacterium ulcerans Agy99 4554165 YP_906896.1 CDS MUL_3208 NC_008611.1 3576126 3576878 D membrane protein; this CDS encodes a 3-oxo-5-alpha-steroid 4- dehydrogenase. This family consists of 3-oxo-5-alpha- steroid 4-dehydrogenases. also known as steroid 5-alpha- reductase, the reaction catalysed by this enzyme is: 3-oxo- 5-alpha-steroid + acceptor <=> 3; hypothetical protein 3576126..3576878 Mycobacterium ulcerans Agy99 4554166 YP_906897.1 CDS MUL_3210 NC_008611.1 3579606 3580364 R membrane protein; hypothetical protein complement(3579606..3580364) Mycobacterium ulcerans Agy99 4554168 YP_906898.1 CDS MUL_5090 NC_008611.1 3582380 3582832 R cytoplasmic protein; hypothetical protein complement(3582380..3582832) Mycobacterium ulcerans Agy99 4554170 YP_906899.1 CDS MUL_3212 NC_008611.1 3582901 3583386 D cytoplasmic protein; function unknown, has HTH motif of a resolvase; hypothetical protein 3582901..3583386 Mycobacterium ulcerans Agy99 4554171 YP_906900.1 CDS MUL_3213 NC_008611.1 3583518 3584852 R cytoplasmic protein; required for the transposition of the insertion element IS2606; transposase for IS2606 complement(3583518..3584852) Mycobacterium ulcerans Agy99 4554190 YP_906901.1 CDS MUL_3214 NC_008611.1 3585032 3585781 R cytoplasmic protein; TatD related DNAse. this family of proteins are related to a large superfamily of metalloenzymes. TatD, a member of this family has been shown experimentally to be a DNAse enzyme.; TatD family deoxyribonuclease complement(3585032..3585781) Mycobacterium ulcerans Agy99 4554191 YP_906902.1 CDS MUL_3215 NC_008611.1 3585781 3587043 R cytoplasmic protein; function unknown, contains PP-loop ATPase domain; hypothetical protein complement(3585781..3587043) Mycobacterium ulcerans Agy99 4554192 YP_906903.1 CDS MUL_3216 NC_008611.1 3587040 3587912 R cytoplasmic protein; hypothetical protein complement(3587040..3587912) Mycobacterium ulcerans Agy99 4554193 YP_906904.1 CDS MUL_3217 NC_008611.1 3587912 3589537 R Detected in the secreted protein fraction by proteomics.; membrane protein; P-loop ATPase complement(3587912..3589537) Mycobacterium ulcerans Agy99 4554194 YP_906905.1 CDS MUL_3218 NC_008611.1 3589714 3590352 R cytoplasmic protein; zinc metalloprotease complement(3589714..3590352) Mycobacterium ulcerans Agy99 4554195 YP_906906.1 CDS MUL_3220 NC_008611.1 3593288 3594622 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 3593288..3594622 Mycobacterium ulcerans Agy99 4554197 YP_906907.1 CDS MUL_3221 NC_008611.1 3595029 3596363 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(3595029..3596363) Mycobacterium ulcerans Agy99 4554198 YP_906908.1 CDS MUL_3224 NC_008611.1 3599809 3601143 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(3599809..3601143) Mycobacterium ulcerans Agy99 4554201 YP_906909.1 CDS MUL_3226 NC_008611.1 3601617 3602603 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3601617..3602603 Mycobacterium ulcerans Agy99 4554203 YP_906910.1 CDS MUL_3229 NC_008611.1 3603946 3605280 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 3603946..3605280 Mycobacterium ulcerans Agy99 4554206 YP_906911.1 CDS MUL_3230 NC_008611.1 3605346 3606950 D cytoplasmic protein; Integration/excision of an unknown mycbacteriophage; prophage integrase 3605346..3606950 Mycobacterium ulcerans Agy99 4554207 YP_906912.1 CDS engA NC_008611.1 3607106 3608515 R synchronizes cellular events by interacting with multiple targets with tandem G-domains; overexpression in Escherichia coli suppresses rrmJ mutation; structural analysis of the Thermotoga maritima ortholog shows different nucleotide binding affinities in the two binding domains; GTP-binding protein EngA complement(3607106..3608515) Mycobacterium ulcerans Agy99 4554209 YP_906913.1 CDS cmk NC_008611.1 3608512 3609240 R Catalyzes the formation of (d)CDP from ATP and (d)CMP; cytidylate kinase complement(3608512..3609240) Mycobacterium ulcerans Agy99 4554210 YP_906914.1 CDS MUL_3234 NC_008611.1 3609311 3610072 R cytoplasmic protein; possible role in ribosome biogenesis and protein translation; pseudouridylate synthase complement(3609311..3610072) Mycobacterium ulcerans Agy99 4554211 YP_906915.1 CDS MUL_3235 NC_008611.1 3610072 3610779 R cytoplasmic protein; transcriptional regulator complement(3610072..3610779) Mycobacterium ulcerans Agy99 4554212 YP_906916.1 CDS MUL_3236 NC_008611.1 3610779 3611606 R cytoplasmic protein; hypothetical protein complement(3610779..3611606) Mycobacterium ulcerans Agy99 4554213 YP_906917.1 CDS MUL_3237 NC_008611.1 3611603 3612559 R cytoplasmic protein; function unknown, possible role in chromosome partioning.; Soj family ATPase complement(3611603..3612559) Mycobacterium ulcerans Agy99 4554214 YP_906918.1 CDS MUL_3238 NC_008611.1 3612640 3612960 R cytoplasmic protein; hypothetical protein complement(3612640..3612960) Mycobacterium ulcerans Agy99 4554215 YP_906919.1 CDS MUL_3239 NC_008611.1 3613546 3614250 D cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 3613546..3614250 Mycobacterium ulcerans Agy99 4554216 YP_906920.1 CDS MUL_3240 NC_008611.1 3614275 3614592 D cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 3614275..3614592 Mycobacterium ulcerans Agy99 4554217 YP_906921.1 CDS MUL_3241 NC_008611.1 3614716 3616050 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(3614716..3616050) Mycobacterium ulcerans Agy99 4554218 YP_906922.1 CDS MUL_3243 NC_008611.1 3618158 3618670 R membrane protein; hypothetical protein complement(3618158..3618670) Mycobacterium ulcerans Agy99 4554220 YP_906923.1 CDS MUL_3245 NC_008611.1 3622188 3623441 D cytoplasmic protein; transcriptional regulator 3622188..3623441 Mycobacterium ulcerans Agy99 4554222 YP_906924.1 CDS MUL_3246 NC_008611.1 3623457 3624212 R Detected in the cytoplasmic and secreted fractions by proteomics.; secreted protein; hypothetical protein complement(3623457..3624212) Mycobacterium ulcerans Agy99 4554223 YP_906925.1 CDS MUL_3247 NC_008611.1 3624487 3626082 D PE_PGRS33; membrane protein; function unknown. seems to influence both cell surface interactions among mycobacteria and the interactions of bacteria with macrophages; PE-PGRS family protein 3624487..3626082 Mycobacterium ulcerans Agy99 4554224 YP_906926.1 CDS snoP NC_008611.1 3626109 3626705 R with PdxST is involved in the biosynthesis of pyridoxal 5'-phosphate; PdxT catalyzes the hydrolysis of glutamine to glutamate and ammonia; PdxS utilizes the ammonia to synthesize pyridoxal 5'-phosphate; glutamine amidotransferase subunit PdxT complement(3626109..3626705) Mycobacterium ulcerans Agy99 4554225 YP_906927.1 CDS tesB2 NC_008611.1 3626713 3627558 R cytoplasmic protein; function unknown, lipolytic enzyme involved in cellular metabolism. can hydrolyze a broad range of acyl- CoA thioesters.; acyl-CoA thioesterase II TesB2 complement(3626713..3627558) Mycobacterium ulcerans Agy99 4554226 YP_906928.1 CDS snzP NC_008611.1 3627568 3628521 R with PdxT forms pyridoxal 5'-phosphate from glutamine, either ribose 5-phosphate or ribulose 5-phosphate, and either glyceraldehyde 3-phosphate or dihydroxyacetone phosphate; pyridoxal biosynthesis lyase PdxS complement(3627568..3628521) Mycobacterium ulcerans Agy99 4554227 YP_906929.1 CDS MUL_3251 NC_008611.1 3628608 3629675 R cytoplasmic protein; function unknown but contains a NUDIX domain; hypothetical protein complement(3628608..3629675) Mycobacterium ulcerans Agy99 4554228 YP_906930.1 CDS pimA NC_008611.1 3629675 3630871 R membrane protein; involved in the first mannosylation step in phosphatidylinositol mannoside biosynthesis (transfer of mannose residues onto pi, leading to the synthesis of phosphatidylinositol monomannoside); alpha-mannosyltransferase PimA complement(3629675..3630871) Mycobacterium ulcerans Agy99 4554229 YP_906931.1 CDS MUL_3253 NC_008611.1 3630799 3631749 R Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA; lipid A biosynthesis lauroyl acyltransferase complement(3630799..3631749) Mycobacterium ulcerans Agy99 4554230 YP_906932.1 CDS pgsA1 NC_008611.1 3631746 3632450 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; catalyzes the transfer of a free alcohol (inositol) onto CDP-diacylglycerol. the product of this putative ORF seems be essential to mycobacteria [catalytic activity: CDP-diacylglycerol + myo-inositol = CMP + phosphatidyl 1D- myo-inositol]; pi synthase PgsA1 complement(3631746..3632450) Mycobacterium ulcerans Agy99 4554231 YP_906933.1 CDS MUL_3255 NC_008611.1 3632458 3633030 R cytoplasmic protein; function unknown, but location and domain identity to hydrlases suggests it could be involved in lipid metabolism.; hypothetical protein complement(3632458..3633030) Mycobacterium ulcerans Agy99 4554232 YP_906934.1 CDS thrS NC_008611.1 3633033 3635108 R catalyzes a two-step reaction, first charging a threonine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; catalyzes the formation of threonyl-tRNA(Thr) from threonine and tRNA(Thr); threonyl-tRNA synthetase complement(3633033..3635108) Mycobacterium ulcerans Agy99 4554233 YP_906935.1 CDS MUL_3257 NC_008611.1 3635236 3635859 R cytoplasmic protein; hypothetical protein complement(3635236..3635859) Mycobacterium ulcerans Agy99 4554234 YP_906936.1 CDS MUL_3259 NC_008611.1 3636884 3637666 D hypothetical protein 3636884..3637666 Mycobacterium ulcerans Agy99 4554236 YP_906937.1 CDS MUL_3261 NC_008611.1 3638364 3638708 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(3638364..3638708) Mycobacterium ulcerans Agy99 4554238 YP_906938.1 CDS MUL_3262 NC_008611.1 3638721 3639146 R membrane protein; hypothetical protein complement(3638721..3639146) Mycobacterium ulcerans Agy99 4554239 YP_906939.1 CDS MUL_3263 NC_008611.1 3639143 3639814 R cytoplasmic protein; transcriptional regulator complement(3639143..3639814) Mycobacterium ulcerans Agy99 4554240 YP_906940.1 CDS fdxA_1 NC_008611.1 3639824 3640171 R cytoplasmic protein; involved in electron transfer.; ferredoxin FdxA complement(3639824..3640171) Mycobacterium ulcerans Agy99 4554241 YP_906941.1 CDS narK3 NC_008611.1 3640339 3641757 D membrane protein; involved in excretion of nitrite produced by the dissimilatory reduction of nitrate. responsible for the translocation of the substrate across the membrane.; integral membrane nitrite extrusion protein NarK3 3640339..3641757 Mycobacterium ulcerans Agy99 4554242 YP_906942.1 CDS MUL_3266 NC_008611.1 3641947 3642771 D cytoplasmic protein; methylation of unknown substrate.; methyltransferase 3641947..3642771 Mycobacterium ulcerans Agy99 4554243 YP_906943.1 CDS MUL_3267 NC_008611.1 3642901 3643794 D cytoplasmic protein; hypothetical protein 3642901..3643794 Mycobacterium ulcerans Agy99 4554244 YP_906944.1 CDS MUL_3269 NC_008611.1 3644638 3645816 R membrane protein; hypothetical protein complement(3644638..3645816) Mycobacterium ulcerans Agy99 4554246 YP_906945.1 CDS MUL_3271 NC_008611.1 3646641 3648068 R membrane protein; hypothetical protein complement(3646641..3648068) Mycobacterium ulcerans Agy99 4554248 YP_906946.1 CDS fadD11_1 NC_008611.1 3649594 3651459 D cytoplasmic protein; involved in lipid metabolism.; long-chain-fatty-acid-CoA ligase, FadD11 3649594..3651459 Mycobacterium ulcerans Agy99 4554250 YP_906947.1 CDS MUL_3275 NC_008611.1 3651521 3652645 D cytoplasmic protein; hypothetical protein 3651521..3652645 Mycobacterium ulcerans Agy99 4554270 YP_906948.1 CDS MUL_3276 NC_008611.1 3652745 3654055 D cytoplasmic protein; function unknown but contains phosphoribosyltransferase domain; hypothetical protein 3652745..3654055 Mycobacterium ulcerans Agy99 4554271 YP_906949.1 CDS MUL_3277 NC_008611.1 3654089 3654370 R cytoplasmic protein; hypothetical protein complement(3654089..3654370) Mycobacterium ulcerans Agy99 4554272 YP_906950.1 CDS MUL_3279 NC_008611.1 3655273 3656652 R cytoplasmic protein; function unknown, but involved in lipid degradation.; fatty-acid-CoA ligase complement(3655273..3656652) Mycobacterium ulcerans Agy99 4554274 YP_906951.1 CDS dedA NC_008611.1 3656884 3657540 D membrane protein; transmembrane protein DedA 3656884..3657540 Mycobacterium ulcerans Agy99 4554275 YP_906952.1 CDS MUL_3282 NC_008611.1 3658333 3658773 D cytoplasmic protein; function unknown but contains identity with a pfam01740 STAS domain. the STAS (after sulphate transporter and antisigma factor antagonist) domain is found in the C terminal region of sulphate transporters and bacterial antisigma factor antagonists. it has been Sug; hypothetical protein 3658333..3658773 Mycobacterium ulcerans Agy99 4554277 YP_906953.1 CDS MUL_3283 NC_008611.1 3658793 3659740 R cytoplasmic protein; it catalyses the conversion of hydrogen peroxide to water and molecular oxygen and is thus involved in the protection of cells from the toxic effects of peroxides.; catalase complement(3658793..3659740) Mycobacterium ulcerans Agy99 4554278 YP_906954.1 CDS MUL_3284 NC_008611.1 3659783 3660082 R cytoplasmic protein; hypothetical protein complement(3659783..3660082) Mycobacterium ulcerans Agy99 4554279 YP_906955.1 CDS MUL_3285 NC_008611.1 3660170 3660529 R cytoplasmic protein; involved in transcriptional mechanism.; ArsR family transcriptional regulator complement(3660170..3660529) Mycobacterium ulcerans Agy99 4554280 YP_906956.1 CDS cadI NC_008611.1 3660631 3661101 D cytoplasmic protein; function unknown, in M. tuberculosis H37Rv this gene induced by cadmium (see hotter et al., 2001); hypothetical protein 3660631..3661101 Mycobacterium ulcerans Agy99 4554281 YP_906957.1 CDS MUL_3287 NC_008611.1 3661181 3661558 D cytoplasmic protein; involved in transcriptional mechanism.; ArsR family transcriptional regulator 3661181..3661558 Mycobacterium ulcerans Agy99 4554282 YP_906958.1 CDS MUL_3289 NC_008611.1 3663205 3664020 D secreted protein; hypothetical protein 3663205..3664020 Mycobacterium ulcerans Agy99 4554284 YP_906959.1 CDS MUL_3291 NC_008611.1 3665315 3666361 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3665315..3666361 Mycobacterium ulcerans Agy99 4554286 YP_906960.1 CDS MUL_3292 NC_008611.1 3666718 3668553 D membrane protein; PE-PGRS family protein 3666718..3668553 Mycobacterium ulcerans Agy99 4554287 YP_906961.1 CDS MUL_3298 NC_008611.1 3670672 3672339 R cytoplasmic protein; hypothetical protein complement(3670672..3672339) Mycobacterium ulcerans Agy99 4554293 YP_906962.1 CDS MUL_3299 NC_008611.1 3672336 3673331 R membrane protein; function unknown but has domain identity with lysophospholipases; hypothetical protein complement(3672336..3673331) Mycobacterium ulcerans Agy99 4554294 YP_906963.1 CDS MUL_3300 NC_008611.1 3673385 3673708 R Detected in the secreted fraction by 2D-LC-MS/MS.; secreted protein; hypothetical protein complement(3673385..3673708) Mycobacterium ulcerans Agy99 4554295 YP_906964.1 CDS clpC2 NC_008611.1 3673817 3674560 D cytoplasmic protein; function unknown, possibly hydrolyzes peptides and/or proteins in presence of ATP.; ATP-dependent protease ATP-binding subunit ClpC2 3673817..3674560 Mycobacterium ulcerans Agy99 4554296 YP_906965.1 CDS MUL_3302 NC_008611.1 3674684 3675199 D secreted protein; hypothetical protein 3674684..3675199 Mycobacterium ulcerans Agy99 4554297 YP_906966.1 CDS MUL_3303 NC_008611.1 3675246 3675704 D cytoplasmic protein; hypothetical protein 3675246..3675704 Mycobacterium ulcerans Agy99 4554317 YP_906967.1 CDS MUL_3304 NC_008611.1 3675682 3676794 R cytoplasmic protein; hypothetical protein complement(3675682..3676794) Mycobacterium ulcerans Agy99 4554318 YP_906968.1 CDS ribD NC_008611.1 3676816 3677598 D cytoplasmic protein; involved in riboflavin biosynthesis (at the second and third steps) converts 2,5-diamino-6-(ribosylamino)- 4(3H)-pyrimidinone 5'-phosphate into 5-amino-6- (ribosylamino)-2,4(1H,3H)-pyrimidinedione 5'-phosphate [catalytic activity 1: 2,5-diamino-6-hydroxy-4-(5- phosphoribosylamino)pyrimidine + H(2)O = 5-amino-6-(5- phosphoribosylamino)uracil + NH(3)] [catalytic activity 2: 5-amino-6-(5-phosphoribitylamino)uracil + NADP(+) = 5- amino-6-(5-phosphoribosylamino)uracil + NADPH]; hypothetical protein 3676816..3677598 Mycobacterium ulcerans Agy99 4554319 YP_906969.1 CDS MUL_3307 NC_008611.1 3679237 3680547 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; transmembrane protein 3679237..3680547 Mycobacterium ulcerans Agy99 4554321 YP_906970.1 CDS MUL_3308 NC_008611.1 3680544 3680984 D cytoplasmic protein; function unknown but contains a SelR domain. these family of reductases are involved in the reduction of methionine sulfoxides in proteins; hypothetical protein 3680544..3680984 Mycobacterium ulcerans Agy99 4554322 YP_906971.1 CDS MUL_3309 NC_008611.1 3680992 3681741 R cytoplasmic protein; methyltransferase complement(3680992..3681741) Mycobacterium ulcerans Agy99 4554323 YP_906972.1 CDS MUL_3310 NC_008611.1 3681738 3682433 R cytoplasmic protein; function unknown but contains identity to pfam06778, chlorite dismutase. this family contains chlorite dismutase enzymes of bacterial and archaeal origin. this enzyme catalyses the disproportionation of chlorite into chloride and oxygen. note that many family memb; hypothetical protein complement(3681738..3682433) Mycobacterium ulcerans Agy99 4554324 YP_906973.1 CDS hemY NC_008611.1 3682439 3683806 R catalyzes the formation of protoporphyrin IX from protoporphyrinogen IX; protoporphyrinogen oxidase complement(3682439..3683806) Mycobacterium ulcerans Agy99 4554325 YP_906974.1 CDS hemE NC_008611.1 3683803 3684864 R catalyzes the formation of coproporphyrinogen from uroporphyrinogen III; uroporphyrinogen decarboxylase complement(3683803..3684864) Mycobacterium ulcerans Agy99 4554326 YP_906975.1 CDS echA15 NC_008611.1 3684987 3685796 D Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase 3684987..3685796 Mycobacterium ulcerans Agy99 4554327 YP_906976.1 CDS MUL_3314 NC_008611.1 3685935 3686516 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein 3685935..3686516 Mycobacterium ulcerans Agy99 4554328 YP_906977.1 CDS MUL_3315 NC_008611.1 3686518 3687825 D cytoplasmic protein; hypothetical protein 3686518..3687825 Mycobacterium ulcerans Agy99 4554329 YP_906978.1 CDS MUL_3317 NC_008611.1 3689324 3690370 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3689324..3690370 Mycobacterium ulcerans Agy99 4554331 YP_906979.1 CDS MUL_3318 NC_008611.1 3690745 3692748 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; PE-PGRS family protein 3690745..3692748 Mycobacterium ulcerans Agy99 4554332 YP_906980.1 CDS dxs1 NC_008611.1 3692791 3694704 R catalyzes the formation of 1-deoxy-D-xylulose 5-phosphate from pyruvate and D-glyceraldehyde 3-phosphate; 1-deoxy-D-xylulose-5-phosphate synthase complement(3692791..3694704) Mycobacterium ulcerans Agy99 4554445 YP_906981.1 CDS MUL_3320 NC_008611.1 3694857 3695351 D cytoplasmic protein; hypothetical protein 3694857..3695351 Mycobacterium ulcerans Agy99 4554446 YP_906982.1 CDS arsA NC_008611.1 3695407 3696696 D membrane protein; thought to be involved in active transport of arsenical compounds across the membrane (export): arsenic resistance by an export mechanism. responsible for the translocation of the substrate across the membrane.; arsenic-transport integral membrane protein ArsA 3695407..3696696 Mycobacterium ulcerans Agy99 4554447 YP_906983.1 CDS MUL_3325 NC_008611.1 3698848 3699513 R membrane protein; thought to be involved in active transport of unidentified antibiotic across the membrane (export): antibiotic resistance by an export mechanism. responsible for the translocation of the substrate across the membrane.; antibiotic ABC transporter integral membrane protein complement(3698848..3699513) Mycobacterium ulcerans Agy99 4554450 YP_906984.1 CDS MUL_3327 NC_008611.1 3700516 3701721 R cytoplasmic protein; function unknown but domain identity suggests this CDS encodes TrmA, a protein related to tRNA (uracil-5-)- methyltransferases.; SAM-dependent methyltransferase complement(3700516..3701721) Mycobacterium ulcerans Agy99 4554452 YP_906985.1 CDS MUL_3328 NC_008611.1 3701718 3703715 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown but domain identity suggests a role in amino acid transport; integral membrane alanine, valine and leucine rich protein complement(3701718..3703715) Mycobacterium ulcerans Agy99 4554453 YP_906986.1 CDS ceoB NC_008611.1 3703848 3704531 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; part of a potassium transport system.; TRK system potassium uptake protein CeoB 3703848..3704531 Mycobacterium ulcerans Agy99 4554454 YP_906987.1 CDS ceoC NC_008611.1 3704584 3705246 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS; cytoplasmic protein; part of a potassium transport system.; TRK system potassium uptake protein CeoC 3704584..3705246 Mycobacterium ulcerans Agy99 4554455 YP_906988.1 CDS MUL_3331 NC_008611.1 3705466 3706137 R membrane protein; integral membrane alanine and leucine rich protein complement(3705466..3706137) Mycobacterium ulcerans Agy99 4554456 YP_906989.1 CDS MUL_3332 NC_008611.1 3706165 3706533 R cytoplasmic protein; hypothetical protein complement(3706165..3706533) Mycobacterium ulcerans Agy99 4554457 YP_906990.1 CDS MUL_3333 NC_008611.1 3706701 3707399 D hypothetical protein 3706701..3707399 Mycobacterium ulcerans Agy99 4554458 YP_906991.1 CDS MUL_3334 NC_008611.1 3707413 3708165 R cytoplasmic protein; hypothetical protein complement(3707413..3708165) Mycobacterium ulcerans Agy99 4554459 YP_906992.1 CDS dut NC_008611.1 3708165 3708629 R catalyzes the formation of dUMP from dUTP; deoxyuridine 5'-triphosphate nucleotidohydrolase complement(3708165..3708629) Mycobacterium ulcerans Agy99 4554460 YP_906993.1 CDS MUL_3336 NC_008611.1 3708655 3709134 D membrane protein; hypothetical protein 3708655..3709134 Mycobacterium ulcerans Agy99 4554461 YP_906994.1 CDS MUL_3337 NC_008611.1 3709139 3709441 R Detected in the secreted fraction by 23D-LC-MS/MS.; cytoplasmic protein; hypothetical protein complement(3709139..3709441) Mycobacterium ulcerans Agy99 4553172 YP_906995.1 CDS MUL_3338 NC_008611.1 3709625 3710278 D secreted protein; hypothetical protein 3709625..3710278 Mycobacterium ulcerans Agy99 4553173 YP_906996.1 CDS suhB NC_008611.1 3710284 3711153 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; in E. coli, SuhB mutation (SuhB2) enhances the synthesis of sigma(32) and suppresses temperature- sensitive growth of the RpoH15 mutant. may affect some step(S) of protein synthesis by facilitating the function of GroE or other heat shock proteins.; extragenic suppressor protein SuhB complement(3710284..3711153) Mycobacterium ulcerans Agy99 4553174 YP_906997.1 CDS ppgK NC_008611.1 3711275 3712069 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; catalyzes the phosphorylation of glucose using polyphosphate or ATP as the phosphoryl donor. GTP, UTP and CTP can replace ATP as phosphoryl donor [catalytic activity: (phosphate)(N) + d-glucose = (phosphate)(N-1) + d-glucose 6-phosphate]; polyphosphate glucokinase PpgK 3711275..3712069 Mycobacterium ulcerans Agy99 4553175 YP_906998.1 CDS sigA NC_008611.1 3712248 3713753 D sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; RNA polymerase sigma factor 3712248..3713753 Mycobacterium ulcerans Agy99 4553176 YP_906999.1 CDS MUL_3344 NC_008611.1 3715933 3716367 D cytoplasmic protein; function unknown but contains pfam01042 domain, endoribonuclease L-PSP. endoribonuclease active on single- stranded mRNA. inhibits protein synthesis by cleavage of mRNA. previously thought to inhibit protein synthesis initiation. this protein may also be involved I; hypothetical protein 3715933..3716367 Mycobacterium ulcerans Agy99 4553178 YP_907000.1 CDS MUL_3345 NC_008611.1 3716295 3716675 R cytoplasmic protein; hypothetical protein complement(3716295..3716675) Mycobacterium ulcerans Agy99 4553179 YP_907001.1 CDS MUL_3346 NC_008611.1 3716802 3716987 D cytoplasmic protein; hypothetical protein 3716802..3716987 Mycobacterium ulcerans Agy99 4553180 YP_907002.1 CDS MUL_3347 NC_008611.1 3717053 3718027 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 3717053..3718027 Mycobacterium ulcerans Agy99 4553181 YP_907003.1 CDS MUL_3348 NC_008611.1 3718028 3718276 R cytoplasmic protein; hypothetical protein complement(3718028..3718276) Mycobacterium ulcerans Agy99 4553182 YP_907004.1 CDS MUL_3349 NC_008611.1 3718313 3718741 D membrane protein; hypothetical protein 3718313..3718741 Mycobacterium ulcerans Agy99 4553183 YP_907005.1 CDS sigB NC_008611.1 3718906 3719877 D sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; sigma factors in this cluster are active during stationary phase; RNA polymerase sigma factor SigB 3718906..3719877 Mycobacterium ulcerans Agy99 4553184 YP_907006.1 CDS ideR NC_008611.1 3720029 3720721 D cytoplasmic protein; transcriptional regulatory protein (repressor and activator), iron-binding repressor of siderophore biosynthesis and iron uptake. seems to regulate a variety of genes encoding a variety of proteins E.G. transporters, proteins involved in siderophore synthesis and iron storage, members of the PE/PPE family, enzymes involved in lipid metabolism, transcriptional regulatory proteins, etc. also activator of BfrA gene.; iron-dependent repressor and activator IdeR 3720029..3720721 Mycobacterium ulcerans Agy99 4553185 YP_907007.1 CDS MUL_3354 NC_008611.1 3723054 3723371 R cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 complement(3723054..3723371) Mycobacterium ulcerans Agy99 4553188 YP_907008.1 CDS MUL_3355 NC_008611.1 3723396 3724100 R cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 complement(3723396..3724100) Mycobacterium ulcerans Agy99 4553189 YP_907009.1 CDS MUL_3356 NC_008611.1 3724695 3725522 R membrane protein; function unknown but contains weak similarity to V8- like Glu-specific endopeptidases involved in amino acid transport and metabolism.; hypothetical protein complement(3724695..3725522) Mycobacterium ulcerans Agy99 4553190 YP_907010.1 CDS MUL_3357 NC_008611.1 3725766 3726743 D Also detected in the extracellular matrix by proteomics.; cytoplasmic protein; hypothetical protein 3725766..3726743 Mycobacterium ulcerans Agy99 4553480 YP_907011.1 CDS MUL_3358 NC_008611.1 3726792 3727817 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; hydrolase 3726792..3727817 Mycobacterium ulcerans Agy99 4553481 YP_907012.1 CDS MUL_3359 NC_008611.1 3727862 3728548 D cytoplasmic protein; function unknown but contains identity to an epimerase involved in phenazine biosynthesis.; hypothetical protein 3727862..3728548 Mycobacterium ulcerans Agy99 4553482 YP_907013.1 CDS MUL_3360 NC_008611.1 3728558 3729043 R cytoplasmic protein; hypothetical protein complement(3728558..3729043) Mycobacterium ulcerans Agy99 4553483 YP_907014.1 CDS nrdR NC_008611.1 3729072 3729536 R Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); membrane protein; transcriptional regulator NrdR complement(3729072..3729536) Mycobacterium ulcerans Agy99 4553484 YP_907015.1 CDS MUL_3362 NC_008611.1 3729699 3730241 R membrane protein; in M. tuberculosis H37Rv thought to be regulated by LexA.; hypothetical protein complement(3729699..3730241) Mycobacterium ulcerans Agy99 4553485 YP_907016.1 CDS lexA NC_008611.1 3730550 3731284 D Represses a number of genes involved in the response to DNA damage; LexA repressor 3730550..3731284 Mycobacterium ulcerans Agy99 4553486 YP_907017.1 CDS MUL_3364 NC_008611.1 3731325 3733628 R Also detected in the cytoplasmic and membrane fractions by LC-MS/MS.; membrane protein; hypothetical protein complement(3731325..3733628) Mycobacterium ulcerans Agy99 4553487 YP_907018.1 CDS fadE20 NC_008611.1 3736509 3737669 R cytoplasmic protein; function unknown, but involved in lipid metabolism.; acyl-CoA dehydrogenase FadE20 complement(3736509..3737669) Mycobacterium ulcerans Agy99 4553490 YP_907019.1 CDS hflX NC_008611.1 3737813 3739147 R cytoplasmic protein; possibly a GTPase, modulating activity of HflK and HflC proteins.; GTP-binding protein HflX complement(3737813..3739147) Mycobacterium ulcerans Agy99 4553491 YP_907020.1 CDS dapF NC_008611.1 3739374 3740246 R involved in lysine biosynthesis; DAP epimerase; produces DL-diaminopimelate from LL-diaminopimelate; diaminopimelate epimerase complement(3739374..3740246) Mycobacterium ulcerans Agy99 4553492 YP_907021.1 CDS miaA NC_008611.1 3740251 3741195 R IPP transferase; isopentenyltransferase; involved in tRNA modification; in Escherichia coli this enzyme catalyzes the addition of a delta2-isopentenyl group from dimethylallyl diphosphate to the N6-nitrogen of adenosine adjacent to the anticodon of tRNA species that read codons starting with uracil; further tRNA modifications may occur; mutations in miaA result in defects in translation efficiency and fidelity; tRNA delta(2)-isopentenylpyrophosphate transferase complement(3740251..3741195) Mycobacterium ulcerans Agy99 4553493 YP_907022.1 CDS MUL_3371 NC_008611.1 3741192 3741887 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; hypothetical protein complement(3741192..3741887) Mycobacterium ulcerans Agy99 4553494 YP_907023.1 CDS MUL_3372 NC_008611.1 3741934 3742911 R membrane protein; hypothetical protein complement(3741934..3742911) Mycobacterium ulcerans Agy99 4553495 YP_907024.1 CDS pknH_2 NC_008611.1 3744494 3746299 D membrane protein; involved in signal transduction (via phosphorylation) thought to be involved in arabinan metabolism, phosphorylating perhaps EmbR [catalytic activity: ATP + a protein = ADP + a phosphoprotein]; Ser/Thr protein kinase 3744494..3746299 Mycobacterium ulcerans Agy99 4553497 YP_907025.1 CDS MUL_3376 NC_008611.1 3746537 3747889 D cytoplasmic protein; hypothetical protein 3746537..3747889 Mycobacterium ulcerans Agy99 4551962 YP_907026.1 CDS MUL_3377 NC_008611.1 3747886 3748497 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(3747886..3748497) Mycobacterium ulcerans Agy99 4551963 YP_907027.1 CDS MUL_3378 NC_008611.1 3748494 3750092 R catalyzes the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine from N6-(dimethylallyl)adenosine (i(6)A); (dimethylallyl)adenosine tRNA methylthiotransferase complement(3748494..3750092) Mycobacterium ulcerans Agy99 4551964 YP_907028.1 CDS recX NC_008611.1 3750167 3750688 R binds RecA and inhibits RecA-mediated DNA strand exchange and ATP hydrolysis and coprotease activities; recombination regulator RecX complement(3750167..3750688) Mycobacterium ulcerans Agy99 4551965 YP_907029.1 CDS recA NC_008611.1 3750654 3751694 R catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs; recombinase A complement(3750654..3751694) Mycobacterium ulcerans Agy99 4551966 YP_907030.1 CDS MUL_3381 NC_008611.1 3751784 3752512 D cytoplasmic protein; hypothetical protein 3751784..3752512 Mycobacterium ulcerans Agy99 4551967 YP_907031.1 CDS MUL_3382 NC_008611.1 3752525 3752731 R cytoplasmic protein; hypothetical protein complement(3752525..3752731) Mycobacterium ulcerans Agy99 4551968 YP_907032.1 CDS MUL_3383 NC_008611.1 3752755 3753921 R function unknown, probably involved in cellular metabolism.; glycosyl transferase family protein complement(3752755..3753921) Mycobacterium ulcerans Agy99 4551969 YP_907033.1 CDS MUL_3384 NC_008611.1 3754017 3754457 D Detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; function unknown but contains a LimA, limonene-1,2- epoxide hydrolase domain.; hypothetical protein 3754017..3754457 Mycobacterium ulcerans Agy99 4551970 YP_907034.1 CDS MUL_3385 NC_008611.1 3754357 3755280 R membrane protein; hypothetical protein complement(3754357..3755280) Mycobacterium ulcerans Agy99 4551971 YP_907035.1 CDS MUL_3386 NC_008611.1 3755296 3756111 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown but domain identity to COG1842, PspA, phage shock protein a (IM30), suppresses sigma54- dependent transcription; hypothetical protein complement(3755296..3756111) Mycobacterium ulcerans Agy99 4551972 YP_907036.1 CDS MUL_3387 NC_008611.1 3756272 3756598 R cytoplasmic protein; possibly involved in transcriptional mechanism.; transcriptional regulatory protein complement(3756272..3756598) Mycobacterium ulcerans Agy99 4551973 YP_907037.1 CDS pgsA3 NC_008611.1 3756738 3757406 R cytoplasmic protein; involved in acidic phospholipid biosynthesis. this protein probably catalyzes the committed step to the synthesis of the acidic phospholipids [catalytic activity: CDP-diacylglycerol + glycerol-3-phosphate = CMP + 3-(3- phosphatidyl)-glycerol 1-phosphate]; PGP synthase PgsA3 complement(3756738..3757406) Mycobacterium ulcerans Agy99 4551974 YP_907038.1 CDS MUL_3389 NC_008611.1 3757392 3757916 D catalyzes the conversion of l-glutamate to a-N-acetyl-l-glutamate in arginine biosynthesis; N-acetylglutamate synthase 3757392..3757916 Mycobacterium ulcerans Agy99 4551975 YP_907039.1 CDS ftsK NC_008611.1 3757928 3760582 R membrane protein; possibly involved in cell division processes; cell division transmembrane protein FtsK complement(3757928..3760582) Mycobacterium ulcerans Agy99 4551976 YP_907040.1 CDS MUL_3391 NC_008611.1 3760717 3761031 D Detected in the cytoplasmic and secreted fractions by 2D-LC-MS/MS.; cytoplasmic protein; hypothetical protein 3760717..3761031 Mycobacterium ulcerans Agy99 4551977 YP_907041.1 CDS fabG NC_008611.1 3761031 3761864 D catalyzes the first of the two reduction steps in the elongation cycle of fatty acid synthesis; 3-ketoacyl-ACP reductase 3761031..3761864 Mycobacterium ulcerans Agy99 4551978 YP_907042.1 CDS MUL_3393 NC_008611.1 3761868 3762740 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; function unknown domain identity suggests this is a methylase involved in secondary metabolism (polyketides); O-methyltransferase 3761868..3762740 Mycobacterium ulcerans Agy99 4551979 YP_907043.1 CDS MUL_3395 NC_008611.1 3763052 3763369 R cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 complement(3763052..3763369) Mycobacterium ulcerans Agy99 4553982 YP_907044.1 CDS MUL_3396 NC_008611.1 3763394 3764098 R cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 complement(3763394..3764098) Mycobacterium ulcerans Agy99 4553983 YP_907045.1 CDS MUL_3398 NC_008611.1 3765449 3766006 R cytoplasmic protein; function unknown but domain identity suggests NADPH- dependent FMN reductase; hypothetical protein complement(3765449..3766006) Mycobacterium ulcerans Agy99 4553985 YP_907046.1 CDS MUL_3399 NC_008611.1 3766037 3766699 D cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator 3766037..3766699 Mycobacterium ulcerans Agy99 4553986 YP_907047.1 CDS MUL_3400 NC_008611.1 3766740 3767615 R cytoplasmic protein; function unknown, but similar to various oxidoreductases and enzymes involved in polyketides synthesis; short chain dehydrogenase complement(3766740..3767615) Mycobacterium ulcerans Agy99 4553987 YP_907048.1 CDS MUL_3401 NC_008611.1 3767688 3768341 R cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator complement(3767688..3768341) Mycobacterium ulcerans Agy99 4553988 YP_907049.1 CDS cyp136A2 NC_008611.1 3768452 3769930 D cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.; cytochrome P450 136A2 Cyp136A2 3768452..3769930 Mycobacterium ulcerans Agy99 4553989 YP_907050.1 CDS MUL_3403 NC_008611.1 3769958 3770488 R cytoplasmic protein; hypothetical protein complement(3769958..3770488) Mycobacterium ulcerans Agy99 4553990 YP_907051.1 CDS MUL_5116 NC_008611.1 3770659 3770952 D cytoplasmic protein; hypothetical protein 3770659..3770952 Mycobacterium ulcerans Agy99 4553991 YP_907052.1 CDS MUL_3404 NC_008611.1 3770994 3771956 R cytoplasmic protein; hypothetical protein complement(3770994..3771956) Mycobacterium ulcerans Agy99 4553992 YP_907053.1 CDS MUL_3406 NC_008611.1 3773567 3774670 R cytoplasmic protein; function unknown, supposed involvement in lipid metabolism.; acetyl-CoA acetyltransferase complement(3773567..3774670) Mycobacterium ulcerans Agy99 4553994 YP_907054.1 CDS MUL_3407 NC_008611.1 3774712 3775758 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(3774712..3775758) Mycobacterium ulcerans Agy99 4553995 YP_907055.1 CDS MUL_3408 NC_008611.1 3776187 3776543 D cytoplasmic protein; function unknown but potential nucleic-acid-binding domain; hypothetical protein 3776187..3776543 Mycobacterium ulcerans Agy99 4553996 YP_907056.1 CDS MUL_3409 NC_008611.1 3776544 3777374 R cytoplasmic protein; methyltransferase complement(3776544..3777374) Mycobacterium ulcerans Agy99 4553997 YP_907057.1 CDS MUL_3410 NC_008611.1 3777540 3778214 D cytoplasmic protein; transcriptional regulator 3777540..3778214 Mycobacterium ulcerans Agy99 4553998 YP_907058.1 CDS fadE22 NC_008611.1 3778248 3780413 R Detected in the membrane fraction by proteomics; cytoplasmic protein; function unknown, but involved in lipid metabolism.; acyl-CoA dehydrogenase FadE22 complement(3778248..3780413) Mycobacterium ulcerans Agy99 4553999 YP_907059.1 CDS MUL_3412 NC_008611.1 3780524 3781282 R Also detected in the membrane fraction by proteomics (LC-MS/MS); cytoplasmic protein; function unknown, supposed involved in cellular metabolism.; short-chain type dehydrogenase/reductase complement(3780524..3781282) Mycobacterium ulcerans Agy99 4554090 YP_907060.1 CDS ligB NC_008611.1 3781391 3782932 D catalyzes the ATP-dependent formation of a phosphodiester at the site of a single-strand break in duplex DNA; ATP-dependent DNA ligase 3781391..3782932 Mycobacterium ulcerans Agy99 4554091 YP_907061.1 CDS MUL_3414 NC_008611.1 3782937 3783548 R membrane protein; hypothetical protein complement(3782937..3783548) Mycobacterium ulcerans Agy99 4554092 YP_907062.1 CDS MUL_3415 NC_008611.1 3783619 3784353 D cytoplasmic protein; involved in transcriptional mechanism; transcriptional regulatory protein 3783619..3784353 Mycobacterium ulcerans Agy99 4554093 YP_907063.1 CDS MUL_5099 NC_008611.1 3785196 3785915 R secreted protein; hypothetical protein complement(3785196..3785915) Mycobacterium ulcerans Agy99 4554095 YP_907064.1 CDS MUL_3418 NC_008611.1 3787036 3788370 R cytoplasmic protein; required for the transposition of the insertion element IS2606; transposase for IS2606 complement(3787036..3788370) Mycobacterium ulcerans Agy99 4554097 YP_907065.1 CDS MUL_3419 NC_008611.1 3788886 3789932 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3788886..3789932 Mycobacterium ulcerans Agy99 4554098 YP_907066.1 CDS dnaK1 NC_008611.1 3792915 3794453 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; acts as a chaperone. involved in induction by stress conditions E.G. heat shock. possibly has an ATPase activity. in M. tuberculosis H37Rv it is regulated positively by SigH and negatively by HspR.; chaperone protein DnaK1 3792915..3794453 Mycobacterium ulcerans Agy99 4554100 YP_907067.1 CDS MUL_3423 NC_008611.1 3794478 3795308 D cytoplasmic protein; short chain dehydrogenase 3794478..3795308 Mycobacterium ulcerans Agy99 4554101 YP_907068.1 CDS MUL_3424 NC_008611.1 3795398 3796033 D cytoplasmic protein; hypothetical protein 3795398..3796033 Mycobacterium ulcerans Agy99 4554102 YP_907069.1 CDS mmr NC_008611.1 3796102 3796425 D membrane protein; involved in transport of multidrugs (tetraphenylphosphonium, erythromycin, ethidium bromide, acriflavine, safranin O, pyronin Y, etc) across the membrane (export): multidrugs resistance by an export mechanism (conferes resistance to toxic compounds by removing them for the cells) responsible for the translocation of the substrate across the membrane.; multidrug-transport integral membrane protein Mmr 3796102..3796425 Mycobacterium ulcerans Agy99 4554103 YP_907070.1 CDS MUL_3426 NC_008611.1 3796422 3797000 D cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein 3796422..3797000 Mycobacterium ulcerans Agy99 4554104 YP_907071.1 CDS MUL_3427 NC_008611.1 3797028 3797405 D cytoplasmic protein; hypothetical protein 3797028..3797405 Mycobacterium ulcerans Agy99 4554105 YP_907072.1 CDS MUL_3428 NC_008611.1 3797492 3798052 D hypothetical protein 3797492..3798052 Mycobacterium ulcerans Agy99 4554106 YP_907073.1 CDS MUL_3429 NC_008611.1 3798205 3799989 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; hydrolase 3798205..3799989 Mycobacterium ulcerans Agy99 4554107 YP_907074.1 CDS putA_2 NC_008611.1 3799986 3801533 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; involved in 4-aminobutyrate (GabA) degradation pathway [catalytic activity: succinate semialdehyde + NAD(P)(+) + H(2)O = succinate + NAD(P)H]; succinate-semialdehyde dehydrogenase [NADP+] dependent, PutA_2 complement(3799986..3801533) Mycobacterium ulcerans Agy99 4554479 YP_907075.1 CDS MUL_3431 NC_008611.1 3801626 3802951 R cytoplasmic protein; function unknown, but involved in lipid degradation.; fatty-acid-CoA ligase complement(3801626..3802951) Mycobacterium ulcerans Agy99 4554480 YP_907076.1 CDS MUL_3433 NC_008611.1 3804486 3805376 R membrane protein; function unknown but contains Cdd pfam03982, DAGAT, diacylglycerol acyltransferase domain.; hypothetical protein complement(3804486..3805376) Mycobacterium ulcerans Agy99 4554482 YP_907077.1 CDS MUL_3434 NC_008611.1 3805658 3806923 D cytoplasmic protein; hypothetical protein 3805658..3806923 Mycobacterium ulcerans Agy99 4554483 YP_907078.1 CDS MUL_3435 NC_008611.1 3806959 3808932 R Good u/s rbs.; function unknown, probably involved in cellular metabolism.; oxidoreductase GMC-type complement(3806959..3808932) Mycobacterium ulcerans Agy99 4554484 YP_907079.1 CDS MUL_3437 NC_008611.1 3809945 3810991 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(3809945..3810991) Mycobacterium ulcerans Agy99 4554486 YP_907080.1 CDS MUL_3438 NC_008611.1 3811889 3813220 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(3811889..3813220) Mycobacterium ulcerans Agy99 4554487 YP_907081.1 CDS MUL_3440 NC_008611.1 3817755 3817907 D cytoplasmic protein; function unknown but has domain similarity to quinoprotein amine dehydrogenases; hypothetical protein 3817755..3817907 Mycobacterium ulcerans Agy99 4554489 YP_907082.1 CDS pgmA NC_008611.1 3818414 3820045 R catalyzes the interconversion of alpha-D-glucose 1-phosphate to alpha-D-glucose 6-phosphate; phosphoglucomutase complement(3818414..3820045) Mycobacterium ulcerans Agy99 4553259 YP_907083.1 CDS MUL_3443 NC_008611.1 3820491 3821555 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3820491..3821555 Mycobacterium ulcerans Agy99 4553260 YP_907084.1 CDS MUL_3446 NC_008611.1 3822332 3823438 D cytoplasmic protein; hypothetical protein 3822332..3823438 Mycobacterium ulcerans Agy99 4553262 YP_907085.1 CDS MUL_3447 NC_008611.1 3823449 3824072 R cytoplasmic protein; hypothetical protein complement(3823449..3824072) Mycobacterium ulcerans Agy99 4553263 YP_907086.1 CDS MUL_3448 NC_008611.1 3824086 3824964 R cytoplasmic protein; oxidoreductase complement(3824086..3824964) Mycobacterium ulcerans Agy99 4553264 YP_907087.1 CDS MUL_3449 NC_008611.1 3825006 3825362 R cytoplasmic protein; hypothetical protein complement(3825006..3825362) Mycobacterium ulcerans Agy99 4553265 YP_907088.1 CDS citE_2 NC_008611.1 3826718 3827653 R cytoplasmic protein; interconversion of acetate and oxaloacetate from citrate [catalytic activity: citrate = acetate + oxaloacetate]; citrate lyase beta subunit, CitE_2 complement(3826718..3827653) Mycobacterium ulcerans Agy99 4553267 YP_907089.1 CDS MUL_3452 NC_008611.1 3827744 3829021 R cytoplasmic protein; function unknown but domain identity suggests transcriptional regulator; hypothetical protein complement(3827744..3829021) Mycobacterium ulcerans Agy99 4553268 YP_907090.1 CDS MUL_3453 NC_008611.1 3829213 3831030 D membrane protein; hypothetical protein 3829213..3831030 Mycobacterium ulcerans Agy99 4553269 YP_907091.1 CDS MUL_3456 NC_008611.1 3834290 3834739 D cytoplasmic protein; hypothetical protein 3834290..3834739 Mycobacterium ulcerans Agy99 4553272 YP_907092.1 CDS MUL_3457 NC_008611.1 3834732 3836543 D cytoplasmic protein; function unknown but domain homology to arylsulfatase a ( AslA )and related enzymes.; hydrolase 3834732..3836543 Mycobacterium ulcerans Agy99 4553273 YP_907093.1 CDS MUL_3458 NC_008611.1 3837162 3837989 R cytoplasmic protein; oxidoreductase complement(3837162..3837989) Mycobacterium ulcerans Agy99 4553275 YP_907094.1 CDS MUL_3459 NC_008611.1 3837998 3838396 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(3837998..3838396) Mycobacterium ulcerans Agy99 4553276 YP_907095.1 CDS MUL_3460 NC_008611.1 3838476 3839939 D cytoplasmic protein; hypothetical protein 3838476..3839939 Mycobacterium ulcerans Agy99 4552758 YP_907096.1 CDS MUL_3461 NC_008611.1 3840320 3840997 D cytoplasmic protein; function unknown, first 70 aa missing WRT to orthologues; hypothetical protein 3840320..3840997 Mycobacterium ulcerans Agy99 4552759 YP_907097.1 CDS MUL_3463 NC_008611.1 3842776 3843420 D secreted protein; hypothetical protein 3842776..3843420 Mycobacterium ulcerans Agy99 4552761 YP_907098.1 CDS MUL_3464 NC_008611.1 3843474 3844241 D cytoplasmic protein; putative regulatory protein 3843474..3844241 Mycobacterium ulcerans Agy99 4552762 YP_907099.1 CDS MUL_3466 NC_008611.1 3845094 3845945 R membrane protein; hypothetical protein complement(3845094..3845945) Mycobacterium ulcerans Agy99 4552764 YP_907100.1 CDS MUL_3467 NC_008611.1 3846293 3847339 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3846293..3847339 Mycobacterium ulcerans Agy99 4552765 YP_907101.1 CDS miaA_1 NC_008611.1 3848339 3849283 D cytoplasmic protein; catalyzes the first step in the biosynthesis of 2- methylthio-N6-(delta(2)-isopentenyl)-adenosine adjacent to the anticodon of several tRNA species [catalytic activity: isopentenyl diphosphate + tRNA = pyrophosphate + tRNA containing 6-isopentenyladenosine]; tRNA delta(2)-isopentenylpyrophosphate transferase MiaA 3848339..3849283 Mycobacterium ulcerans Agy99 4552767 YP_907102.1 CDS MUL_3470 NC_008611.1 3849370 3850350 D functions in degradation of stringent response intracellular messenger ppGpp; in Escherichia coli this gene is co-transcribed with the toxin/antitoxin genes mazEF; activity of MazG is inhibited by MazEF in vitro; ppGpp inhibits mazEF expression; MazG thus works in limiting the toxic activity of the MazF toxin induced during starvation; MazG also interacts with the GTPase protein Era; nucleoside triphosphate pyrophosphohydrolase 3849370..3850350 Mycobacterium ulcerans Agy99 4552768 YP_907103.1 CDS MUL_3472 NC_008611.1 3851720 3852922 R cytoplasmic protein; NADP-dependent oxidoreductase complement(3851720..3852922) Mycobacterium ulcerans Agy99 4552770 YP_907104.1 CDS MUL_3474 NC_008611.1 3853907 3855130 R cytoplasmic protein; dehydrogenase complement(3853907..3855130) Mycobacterium ulcerans Agy99 4552772 YP_907105.1 CDS MUL_3475 NC_008611.1 3855177 3855653 D cytoplasmic protein; transcriptional regulatory protein 3855177..3855653 Mycobacterium ulcerans Agy99 4552773 YP_907106.1 CDS MUL_3476 NC_008611.1 3855911 3857050 D secreted protein; hypothetical protein 3855911..3857050 Mycobacterium ulcerans Agy99 4552774 YP_907107.1 CDS MUL_3477 NC_008611.1 3857064 3857552 R secreted protein; hypothetical protein complement(3857064..3857552) Mycobacterium ulcerans Agy99 4552775 YP_907108.1 CDS MUL_3478 NC_008611.1 3857853 3858095 R secreted protein; hypothetical protein complement(3857853..3858095) Mycobacterium ulcerans Agy99 4552250 YP_907109.1 CDS MUL_3479 NC_008611.1 3858242 3859090 D cytoplasmic protein; hypothetical protein 3858242..3859090 Mycobacterium ulcerans Agy99 4552251 YP_907110.1 CDS MUL_3480 NC_008611.1 3859370 3859987 D cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 3859370..3859987 Mycobacterium ulcerans Agy99 4552252 YP_907111.1 CDS MUL_3481 NC_008611.1 3860072 3860416 D cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 3860072..3860416 Mycobacterium ulcerans Agy99 4552253 YP_907112.1 CDS MUL_3483 NC_008611.1 3862725 3863171 R cytoplasmic protein; function unknown but has domain similarity to predicted flavin-nucleotide-binding proteins; hypothetical protein complement(3862725..3863171) Mycobacterium ulcerans Agy99 4552255 YP_907113.1 CDS glsA NC_008611.1 3863241 3864203 R catalyzes the formation of glutamate from glutamine; glutaminase complement(3863241..3864203) Mycobacterium ulcerans Agy99 4552256 YP_907114.1 CDS MUL_3486 NC_008611.1 3864892 3865938 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(3864892..3865938) Mycobacterium ulcerans Agy99 4552258 YP_907115.1 CDS MUL_3487 NC_008611.1 3866391 3867725 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(3866391..3867725) Mycobacterium ulcerans Agy99 4552259 YP_907116.1 CDS MUL_3490 NC_008611.1 3869647 3870588 D cytoplasmic protein; function unknown but contains lysophospholipase domain; hypothetical protein 3869647..3870588 Mycobacterium ulcerans Agy99 4552263 YP_907117.1 CDS MUL_3491 NC_008611.1 3870998 3871432 D cytoplasmic protein; function unknown, contains predicted nucleic acid- binding pin domain; hypothetical protein 3870998..3871432 Mycobacterium ulcerans Agy99 4552264 YP_907118.1 CDS MUL_3492 NC_008611.1 3871628 3872032 R membrane protein; hypothetical protein complement(3871628..3872032) Mycobacterium ulcerans Agy99 4552265 YP_907119.1 CDS wag31 NC_008611.1 3872117 3872911 R Detected in the membrane and the cytoplamic fractions by proteomics.; secreted protein; function unknown but corresponds to antigen 84 of mycobacterium tuberculosis (Wag31) (see hermans et al., 1995) predicted to contain significant amount of coiled coil structure.; secreted antigen Wag31 complement(3872117..3872911) Mycobacterium ulcerans Agy99 4550964 YP_907120.1 CDS MUL_3494 NC_008611.1 3873168 3873458 R membrane protein; hypothetical protein complement(3873168..3873458) Mycobacterium ulcerans Agy99 4550965 YP_907121.1 CDS MUL_3495 NC_008611.1 3873636 3874307 R cytoplasmic protein; hypothetical protein complement(3873636..3874307) Mycobacterium ulcerans Agy99 4550966 YP_907122.1 CDS MUL_3496 NC_008611.1 3874372 3875148 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(3874372..3875148) Mycobacterium ulcerans Agy99 4550967 YP_907123.1 CDS yfiH NC_008611.1 3875154 3875942 R Detected in the membrane fraction by proteomics.; membrane protein; membrane protein YfiH complement(3875154..3875942) Mycobacterium ulcerans Agy99 4550968 YP_907124.1 CDS ftsZ NC_008611.1 3875953 3877116 R GTPase; similar structure to tubulin; forms ring-shaped polymers at the site of cell division; other proteins such as FtsA, ZipA, and ZapA, interact with and regulate FtsZ function; cell division protein FtsZ complement(3875953..3877116) Mycobacterium ulcerans Agy99 4550969 YP_907125.1 CDS ftsQ NC_008611.1 3877363 3878319 R cytoplasmic protein; this protein may be involved in septum formation (by similarity); cell division protein FtsQ complement(3877363..3878319) Mycobacterium ulcerans Agy99 4550970 YP_907126.1 CDS murC NC_008611.1 3878316 3879782 R Catalyzes the formation of UDP-N-acetylmuramoyl-L-alanine from UDP-N-acetylmuramate and L-alanine in peptidoglycan synthesis; UDP-N-acetylmuramate--L-alanine ligase complement(3878316..3879782) Mycobacterium ulcerans Agy99 4550971 YP_907127.1 CDS murG NC_008611.1 3879779 3880978 R UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase; involved in cell wall formation; inner membrane-associated; last step of peptidoglycan synthesis; undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase complement(3879779..3880978) Mycobacterium ulcerans Agy99 4550972 YP_907128.1 CDS ftsW NC_008611.1 3880975 3882606 R membrane protein; function unknown function in cell division; FtsW-like protein FtsW complement(3880975..3882606) Mycobacterium ulcerans Agy99 4550973 YP_907129.1 CDS murD NC_008611.1 3882613 3884079 R UDP-N-acetylmuramoylalanine--D-glutamate ligase; involved in peptidoglycan biosynthesis; cytoplasmic; catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine during cell wall formation; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase complement(3882613..3884079) Mycobacterium ulcerans Agy99 4550974 YP_907130.1 CDS mraY NC_008611.1 3884081 3885160 R First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan; phospho-N-acetylmuramoyl-pentapeptide- transferase complement(3884081..3885160) Mycobacterium ulcerans Agy99 4550975 YP_907131.1 CDS murF NC_008611.1 3885157 3886734 R Detected in the membrane fraction by proteomics.; membrane protein; involved in cell wall formation; peptidoglycan biosynthesis.; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6- diaminopimelate-D-alanyl-D-alanyl ligase MurF complement(3885157..3886734) Mycobacterium ulcerans Agy99 4550976 YP_907132.1 CDS murE NC_008611.1 3886731 3888410 R involved in cell wall formation; peptidoglycan synthesis; cytoplasmic enzyme; catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-aceylmuramoyl-l-alanyl-d-glutamate; UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase complement(3886731..3888410) Mycobacterium ulcerans Agy99 4550977 YP_907133.1 CDS MUL_3507 NC_008611.1 3888660 3890477 R membrane protein; PE-PGRS family protein complement(3888660..3890477) Mycobacterium ulcerans Agy99 4550978 YP_907134.1 CDS pbpB NC_008611.1 3890728 3892803 R membrane protein; involved in peptidoglycan biosynthesis.; penicillin-binding membrane protein PbpB complement(3890728..3892803) Mycobacterium ulcerans Agy99 4550979 YP_907135.1 CDS MUL_3509 NC_008611.1 3892800 3894071 R membrane protein; proline rich membrane protein complement(3892800..3894071) Mycobacterium ulcerans Agy99 4550980 YP_907136.1 CDS mraW NC_008611.1 3894068 3895255 R cytoplasmic protein; S-adenosyl-methyltransferase MraW complement(3894068..3895255) Mycobacterium ulcerans Agy99 4550981 YP_907137.1 CDS MUL_3511 NC_008611.1 3895257 3895688 R MraZ; UPF0040; crystal structure shows similarity to AbrB; cell division protein MraZ complement(3895257..3895688) Mycobacterium ulcerans Agy99 4552658 YP_907138.1 CDS MUL_3512 NC_008611.1 3896097 3896501 R membrane protein; hypothetical protein complement(3896097..3896501) Mycobacterium ulcerans Agy99 4552659 YP_907139.1 CDS MUL_3513 NC_008611.1 3896804 3897442 D cytoplasmic protein; function unknown but contains acyl-CoA N- acyltransferase superfamily domain; hypothetical protein 3896804..3897442 Mycobacterium ulcerans Agy99 4552660 YP_907140.1 CDS lppM NC_008611.1 3897592 3898239 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; lipoprotein LppM 3897592..3898239 Mycobacterium ulcerans Agy99 4552661 YP_907141.1 CDS MUL_3515 NC_008611.1 3898285 3899181 R cytoplasmic protein; hypothetical protein complement(3898285..3899181) Mycobacterium ulcerans Agy99 4552662 YP_907142.1 CDS idsA2 NC_008611.1 3899503 3900525 D Detected in the membrane fraction by proteomics.; cytoplasmic protein; involved in lipid biosynthesis.; geranylgeranyl pyrophosphate synthase 3899503..3900525 Mycobacterium ulcerans Agy99 4552663 YP_907143.1 CDS MUL_3517 NC_008611.1 3900662 3902110 D membrane protein; hypothetical protein 3900662..3902110 Mycobacterium ulcerans Agy99 4552664 YP_907144.1 CDS MUL_3518 NC_008611.1 3902148 3902552 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; regulatory protein complement(3902148..3902552) Mycobacterium ulcerans Agy99 4552665 YP_907145.1 CDS pknL NC_008611.1 3902643 3903938 D membrane protein; involved in signal transduction (via phosphorylation); Ser/Thr protein kinase 3902643..3903938 Mycobacterium ulcerans Agy99 4552666 YP_907146.1 CDS MUL_3520 NC_008611.1 3904610 3905941 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 3904610..3905941 Mycobacterium ulcerans Agy99 4552667 YP_907147.1 CDS MUL_3522 NC_008611.1 3907143 3908198 R cytoplasmic protein; HMGR is a tightly regulated enzyme, which catalyzes the synthesis of coenzyme a and mevalonate in isoprenoid synthesis. in mammals, this is the rate limiting committed step in cholesterol biosynthesis. bacteria, such as pseudomonas mevalonii, which rely solely on mevalonate for their carbon source, catalyze the reverse reaction, using an NAD-dependent HMGR to deacetylate mevalonate into 3- hydroxy-3-methylglutaryl-CoA.; hydroxymethylglutaryl-coenzyme a (HMG-CoA) reductase complement(3907143..3908198) Mycobacterium ulcerans Agy99 4552669 YP_907148.1 CDS MUL_3523 NC_008611.1 3908222 3909334 R membrane protein; part of the mevalonate pathway for isoprenoid synthesis. possible regulatory role in mevalonate metabolism. converts (R)-5-phosphomevalonate to (R)-5- diphosphomevalonate.; phosphomevalonate kinase complement(3908222..3909334) Mycobacterium ulcerans Agy99 4552670 YP_907149.1 CDS MUL_3524 NC_008611.1 3909331 3910341 R cytoplasmic protein; key enzyme in isoprenoid synthesis. converts (R)-5- diphosphomevalonate to phosphate + isopentenyl diphosphate + CO(2); diphosphomevalonate decarboxylase complement(3909331..3910341) Mycobacterium ulcerans Agy99 4552671 YP_907150.1 CDS erg12 NC_008611.1 3910338 3911417 R cytoplasmic protein; converts (R)-mevalonate to (R)-5-phosphomevalonate (ATP dependant); mevalonate kinase, Erg12 complement(3910338..3911417) Mycobacterium ulcerans Agy99 4552672 YP_907151.1 CDS MUL_3527 NC_008611.1 3911690 3912736 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(3911690..3912736) Mycobacterium ulcerans Agy99 4552674 YP_907152.1 CDS fabH_1 NC_008611.1 3916481 3917482 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; involved in fatty acid biosynthesis. catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl- acp. kas III catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. possesses both acetoacetyl-acp synthase and acetyl transacylase activities [catalytic activity: acyl- [acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]]; 3-oxoacyl-ACP synthase 3916481..3917482 Mycobacterium ulcerans Agy99 4553928 YP_907153.1 CDS aroG_1 NC_008611.1 3919269 3920657 R cytoplasmic protein; involved in chorismate biosynthesis; 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase complement(3919269..3920657) Mycobacterium ulcerans Agy99 4553930 YP_907154.1 CDS MUL_3534 NC_008611.1 3920706 3921185 R cytoplasmic protein; hypothetical protein complement(3920706..3921185) Mycobacterium ulcerans Agy99 4553931 YP_907155.1 CDS MUL_3535 NC_008611.1 3921169 3922008 R membrane protein; hypothetical protein complement(3921169..3922008) Mycobacterium ulcerans Agy99 4553932 YP_907156.1 CDS MUL_3536 NC_008611.1 3922153 3923436 D membrane protein; hypothetical protein 3922153..3923436 Mycobacterium ulcerans Agy99 4553933 YP_907157.1 CDS MUL_3537 NC_008611.1 3923437 3924168 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; transfer of fatty acyl groups; 1-acylglycerol-3-phosphate O-acyltransferase complement(3923437..3924168) Mycobacterium ulcerans Agy99 4553934 YP_907158.1 CDS MUL_3538 NC_008611.1 3924256 3924645 R cytoplasmic protein; hypothetical protein complement(3924256..3924645) Mycobacterium ulcerans Agy99 4553935 YP_907159.1 CDS MUL_3539 NC_008611.1 3924642 3925793 R cytoplasmic protein; function unknown but domain identity suggests it is an anion-transporting ATPase.; hypothetical protein complement(3924642..3925793) Mycobacterium ulcerans Agy99 4553936 YP_907160.1 CDS MUL_3540 NC_008611.1 3925913 3926347 R cytoplasmic protein; hypothetical protein complement(3925913..3926347) Mycobacterium ulcerans Agy99 4553937 YP_907161.1 CDS MUL_3541 NC_008611.1 3926444 3926833 R cytoplasmic protein; hypothetical protein complement(3926444..3926833) Mycobacterium ulcerans Agy99 4553938 YP_907162.1 CDS fadD15 NC_008611.1 3927041 3928843 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown, but involved in lipid metabolism.; long-chain-fatty-acid-CoA ligase FadD15 3927041..3928843 Mycobacterium ulcerans Agy99 4553939 YP_907163.1 CDS MUL_3543 NC_008611.1 3928911 3930068 R cytoplasmic protein; glycosyltransferase complement(3928911..3930068) Mycobacterium ulcerans Agy99 4553940 YP_907164.1 CDS MUL_3545 NC_008611.1 3931010 3932176 R secreted protein; hypothetical protein complement(3931010..3932176) Mycobacterium ulcerans Agy99 4553942 YP_907165.1 CDS MUL_3546 NC_008611.1 3932364 3932618 R cytoplasmic protein; hypothetical protein complement(3932364..3932618) Mycobacterium ulcerans Agy99 4553943 YP_907166.1 CDS trpD NC_008611.1 3934890 3935993 R Catalyzes the conversion of N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate; anthranilate phosphoribosyltransferase complement(3934890..3935993) Mycobacterium ulcerans Agy99 4554545 YP_907167.1 CDS ctaE NC_008611.1 3936144 3936755 D cytoplasmic protein; thought to be involved in aerobic respiration.; cytochrome C oxidase (subunit III) CtaE 3936144..3936755 Mycobacterium ulcerans Agy99 4554546 YP_907168.1 CDS qcrC NC_008611.1 3936808 3937713 D membrane protein; plays a role in aerobic respiration; ubiquinol-cytochrome C reductase QcrC 3936808..3937713 Mycobacterium ulcerans Agy99 4554547 YP_907169.1 CDS qcrA NC_008611.1 3937710 3938894 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS) Also detected in the cytoplasmic fraction by 2D- LC-MS/MS; membrane protein; role in respiration; Rieske iron-sulfur protein QcrA 3937710..3938894 Mycobacterium ulcerans Agy99 4554548 YP_907170.1 CDS qcrB NC_008611.1 3938891 3940576 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; role in respiration; ubiquinol-cytochrome C reductase QcrB 3938891..3940576 Mycobacterium ulcerans Agy99 4554549 YP_907171.1 CDS MUL_3554 NC_008611.1 3941022 3941660 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; transmembrane protein complement(3941022..3941660) Mycobacterium ulcerans Agy99 4554550 YP_907172.1 CDS mmpS3 NC_008611.1 3941660 3942607 R membrane protein; membrane protein MmpS3 complement(3941660..3942607) Mycobacterium ulcerans Agy99 4554551 YP_907173.1 CDS MUL_3556 NC_008611.1 3942790 3943209 R cytoplasmic protein; integral membrane protein complement(3942790..3943209) Mycobacterium ulcerans Agy99 4554552 YP_907174.1 CDS ctaC NC_008611.1 3943218 3944321 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in aerobic respiration. subunit I and II form the functional core of the enzyme complex. electrons originating in cytochrome C are transferred via HemE A and Cu(A) to the binuclear center formed by HemE A3 and Cu(B) (by similarity); transmembrane cytochrome C oxidase (subunit II) CtaC complement(3943218..3944321) Mycobacterium ulcerans Agy99 4554553 YP_907175.1 CDS asnB NC_008611.1 3944718 3946712 D cytoplasmic protein; asparagine biosynthesis; asparagine synthetase AsnB 3944718..3946712 Mycobacterium ulcerans Agy99 4554554 YP_907176.1 CDS cbhK NC_008611.1 3946725 3947699 R Detected in the cytoplamic fraction by LCMSMS. Also detected in the membrane fraction by proteomics (2D-LC- MS/MS); cytoplasmic protein; phosphorylation of carbohydrates; carbohydrate kinase CbhK complement(3946725..3947699) Mycobacterium ulcerans Agy99 4554555 YP_907177.1 CDS MUL_3560 NC_008611.1 3947938 3948579 D Detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; hypothetical protein 3947938..3948579 Mycobacterium ulcerans Agy99 4554556 YP_907178.1 CDS MUL_3561 NC_008611.1 3948620 3948976 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown but domain identity shows similarity to the HesB gene (function unknown) but which is expressed only under nitrogen fixation conditions.; hypothetical protein complement(3948620..3948976) Mycobacterium ulcerans Agy99 4554557 YP_907179.1 CDS MUL_3562 NC_008611.1 3949063 3950139 R membrane protein; function unknown role in carbohydrate transport and metabolism; glycerate kinase complement(3949063..3950139) Mycobacterium ulcerans Agy99 4554558 YP_907180.1 CDS MUL_3563 NC_008611.1 3950302 3951006 D membrane protein; transmembrane protein 3950302..3951006 Mycobacterium ulcerans Agy99 4554559 YP_907181.1 CDS cobU NC_008611.1 3951035 3951595 D cytoplasmic protein; involved in cobalamin biosynthesis.; bifunctional cobalamin biosynthesis protein CobU 3951035..3951595 Mycobacterium ulcerans Agy99 4554560 YP_907182.1 CDS cobT NC_008611.1 3951592 3952668 D catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide and 5,6-dimethylbenzimidazole; nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase 3951592..3952668 Mycobacterium ulcerans Agy99 4554561 YP_907183.1 CDS cobS NC_008611.1 3952665 3953423 D catalyzes the formation of adenosylcobalamin from Ado-cobinamide-GDP and alpha-ribazole; cobalamin synthase 3952665..3953423 Mycobacterium ulcerans Agy99 4554562 YP_907184.1 CDS MUL_3567 NC_008611.1 3953656 3954282 R cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 complement(3953656..3954282) Mycobacterium ulcerans Agy99 4553401 YP_907185.1 CDS MUL_3568 NC_008611.1 3954352 3954702 R cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 complement(3954352..3954702) Mycobacterium ulcerans Agy99 4553402 YP_907186.1 CDS MUL_3569 NC_008611.1 3955072 3956484 D membrane protein; function unknown but domain identity with chloride channel superfamily; hypothetical protein 3955072..3956484 Mycobacterium ulcerans Agy99 4553403 YP_907187.1 CDS ilvE NC_008611.1 3956476 3957582 R catalyzes the transamination of the branched-chain amino acids to their respective alpha-keto acids; branched-chain amino acid aminotransferase complement(3956476..3957582) Mycobacterium ulcerans Agy99 4553404 YP_907188.1 CDS gcvT NC_008611.1 3957636 3958739 R catalyzes the transfer of a methylene carbon from the methylamine-loaded GcvH protein to tetrahydrofolate, causing the release of ammonia and the generation of reduced GcvH protein; glycine cleavage system aminomethyltransferase T complement(3957636..3958739) Mycobacterium ulcerans Agy99 4553405 YP_907189.1 CDS pepB NC_008611.1 3959940 3961499 D catalyzes the removal of N-terminal amino acids preferably leucine from various peptides; leucyl aminopeptidase 3959940..3961499 Mycobacterium ulcerans Agy99 4553407 YP_907190.1 CDS ephD NC_008611.1 3961962 3963746 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; thought to be involved in detoxification reactions following oxidative damage to lipids.; short chain dehydrogenase complement(3961962..3963746) Mycobacterium ulcerans Agy99 4553408 YP_907191.1 CDS sucB NC_008611.1 3963975 3965741 D Detected in the cytoplasmic, the extracellular matrix, and the membrane fraction by proteomics.; cytoplasmic protein; involved in tricarboxylic acid cycle; converts 2- oxoglutarate to succinyl-CoA and CO2; dihydrolipoamide acetyltransferase 3963975..3965741 Mycobacterium ulcerans Agy99 4553409 YP_907192.1 CDS MUL_3576 NC_008611.1 3965746 3966657 D function unknown but domain identity with NAD dependent sugar epimerases; hypothetical protein 3965746..3966657 Mycobacterium ulcerans Agy99 4553410 YP_907193.1 CDS MUL_3577 NC_008611.1 3966443 3967684 R membrane protein; probably involved in active transport of substrate across the membrane (export) responsible for energy coupling to the transport system.; ABC transporter ATP-binding protein complement(3966443..3967684) Mycobacterium ulcerans Agy99 4553411 YP_907194.1 CDS MUL_3579 NC_008611.1 3969592 3970638 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3969592..3970638 Mycobacterium ulcerans Agy99 4553413 YP_907195.1 CDS MUL_3581 NC_008611.1 3971923 3972276 R cytoplasmic protein; DNA integration. this sequence contains a DNA breaking-rejoining catalytic core found in phage integrase genes.; phage-related integrase complement(3971923..3972276) Mycobacterium ulcerans Agy99 4553366 YP_907196.1 CDS MUL_3582 NC_008611.1 3972570 3973187 R membrane protein; hypothetical protein complement(3972570..3973187) Mycobacterium ulcerans Agy99 4553367 YP_907197.1 CDS MUL_5087 NC_008611.1 3973525 3973791 R cytoplasmic protein; hypothetical protein complement(3973525..3973791) Mycobacterium ulcerans Agy99 4553368 YP_907198.1 CDS MUL_3584 NC_008611.1 3974228 3975274 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3974228..3975274 Mycobacterium ulcerans Agy99 4553369 YP_907199.1 CDS MUL_3585 NC_008611.1 3975756 3976088 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 3975756..3976088 Mycobacterium ulcerans Agy99 4553371 YP_907200.1 CDS MUL_3586 NC_008611.1 3976103 3976528 D membrane protein; hypothetical protein 3976103..3976528 Mycobacterium ulcerans Agy99 4553372 YP_907201.1 CDS lpqL NC_008611.1 3976661 3978172 D membrane protein; function unknown, hydrolyzes peptides and/or proteins.; lipoprotein aminopeptidase LpqL 3976661..3978172 Mycobacterium ulcerans Agy99 4553373 YP_907202.1 CDS lpqM NC_008611.1 3979755 3981248 D secreted protein; function unknown, possibly hydrolyzes peptides and/or proteins.; lipoprotein peptidase LpqM 3979755..3981248 Mycobacterium ulcerans Agy99 4553375 YP_907203.1 CDS MUL_3590 NC_008611.1 3981279 3984515 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; function unknown, but may be involved in efflux system (probably sugar or drug transport); transmembrane transport protein complement(3981279..3984515) Mycobacterium ulcerans Agy99 4553376 YP_907204.1 CDS MUL_3591 NC_008611.1 3985251 3986297 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3985251..3986297 Mycobacterium ulcerans Agy99 4553377 YP_907205.1 CDS MUL_3593 NC_008611.1 3988189 3989235 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 3988189..3989235 Mycobacterium ulcerans Agy99 4553379 YP_907206.1 CDS MUL_3594 NC_008611.1 3989272 3990798 D cytoplasmic protein; function unknown but contains a N-term CheY-like response regulator receiver domain and C-term adenylyl/guanylyl cyclase catalytic domain.; adenylylate/guanylate cyclase 3989272..3990798 Mycobacterium ulcerans Agy99 4553380 YP_907207.1 CDS MUL_3595 NC_008611.1 3990791 3991105 R membrane protein; hypothetical protein complement(3990791..3991105) Mycobacterium ulcerans Agy99 4553381 YP_907208.1 CDS MUL_3596 NC_008611.1 3991153 3991377 R membrane protein; hypothetical protein complement(3991153..3991377) Mycobacterium ulcerans Agy99 4553297 YP_907209.1 CDS MUL_3597 NC_008611.1 3991479 3992822 D cytoplasmic protein; function unknown but has two TetR DNA-binding domains; putative regulatory protein 3991479..3992822 Mycobacterium ulcerans Agy99 4553298 YP_907210.1 CDS thiD NC_008611.1 3993365 3994192 R catalyzes the formation of 4-amino-2-methyl-5-diphosphomethylpyrimidine; phosphomethylpyrimidine kinase complement(3993365..3994192) Mycobacterium ulcerans Agy99 4553300 YP_907211.1 CDS thiC NC_008611.1 3994189 3995829 R required for the synthesis of the hydromethylpyrimidine moiety of thiamine; thiamine biosynthesis protein ThiC complement(3994189..3995829) Mycobacterium ulcerans Agy99 4553301 YP_907212.1 CDS MUL_3601 NC_008611.1 3995829 3996749 D cytoplasmic protein; function unknown but may be involved in signal transduction pathway as it contains a PAS-like central senor domain and an Antar domain.; putative regulatory protein 3995829..3996749 Mycobacterium ulcerans Agy99 4553302 YP_907213.1 CDS MUL_3602 NC_008611.1 3996880 3997275 D cytoplasmic protein; function unknown but domain identity shared with the anti-sigma factor antagonist superfamily SpoIIAA; anti sigma factor antagonist 3996880..3997275 Mycobacterium ulcerans Agy99 4553303 YP_907214.1 CDS MUL_3604 NC_008611.1 4001165 4001443 R cytoplasmic protein; hypothetical protein complement(4001165..4001443) Mycobacterium ulcerans Agy99 4553305 YP_907215.1 CDS MUL_3606 NC_008611.1 4004641 4005975 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 4004641..4005975 Mycobacterium ulcerans Agy99 4553307 YP_907216.1 CDS MUL_3609 NC_008611.1 4009684 4011018 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 4009684..4011018 Mycobacterium ulcerans Agy99 4553310 YP_907217.1 CDS glyS NC_008611.1 4011196 4012575 R Catalyzes a two-step reaction, first charging a glycine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; glycyl-tRNA synthetase complement(4011196..4012575) Mycobacterium ulcerans Agy99 4553311 YP_907218.1 CDS MUL_3611 NC_008611.1 4012780 4013175 D cytoplasmic protein; involved in transcriptional mechanism.; ArsR family transcriptional regulator 4012780..4013175 Mycobacterium ulcerans Agy99 4553312 YP_907219.1 CDS furB NC_008611.1 4013172 4013585 D cytoplasmic protein; acts as a global negative controlling element, employing fe(2+) as a cofactor to bind the operator of the repressed genes. regulates the expression of several outer- membrane proteins including the iron transport operon (by similarity); ferric uptake regulation protein FurB 4013172..4013585 Mycobacterium ulcerans Agy99 4553277 YP_907220.1 CDS MUL_3613 NC_008611.1 4013582 4014022 R cytoplasmic protein; hypothetical protein complement(4013582..4014022) Mycobacterium ulcerans Agy99 4553278 YP_907221.1 CDS MUL_3614 NC_008611.1 4014022 4014912 R catalyzes the formation of undecaprenyl pyrophosphate from isopentenyl pyrophosphate; undecaprenyl pyrophosphate synthase complement(4014022..4014912) Mycobacterium ulcerans Agy99 4553279 YP_907222.1 CDS recO NC_008611.1 4014905 4015702 R involved in DNA repair and RecFOR pathway recombination; RecFOR proteins displace ssDNA-binding protein and facilitate the production of RecA-coated ssDNA; DNA repair protein RecO complement(4014905..4015702) Mycobacterium ulcerans Agy99 4553280 YP_907223.1 CDS amiA2 NC_008611.1 4015764 4017263 D catalyzes the hydrolysis of a monocarboxylic acid amid to form a monocarboxylate and ammonia; amidase 4015764..4017263 Mycobacterium ulcerans Agy99 4553281 YP_907224.1 CDS era NC_008611.1 4017260 4018162 R Era; Escherichia coli Ras-like protein; Bex; Bacillus Era-complementing segment; essential protein in Escherichia coli that is involved in many cellular processes; GTPase; binds the cell membrane through apparent C-terminal domain; mutants are arrested during the cell cycle; Streptococcus pneumoniae Era binds to RNA and Escherichia coli Era binds 16S rRNA and 30S ribosome; GTP-binding protein Era complement(4017260..4018162) Mycobacterium ulcerans Agy99 4553282 YP_907225.1 CDS MUL_3618 NC_008611.1 4018152 4018382 R cytoplasmic protein; hypothetical protein complement(4018152..4018382) Mycobacterium ulcerans Agy99 4553283 YP_907226.1 CDS MUL_3620 NC_008611.1 4019671 4020207 R cytoplasmic protein; putative metalloprotease complement(4019671..4020207) Mycobacterium ulcerans Agy99 4553285 YP_907227.1 CDS phoH1 NC_008611.1 4020226 4021284 R cytoplasmic protein; PhoH-like protein PhoH1 complement(4020226..4021284) Mycobacterium ulcerans Agy99 4553286 YP_907228.1 CDS MUL_3622 NC_008611.1 4021352 4022074 R cytoplasmic protein; function unknown but contains tetracycline repressor-like C-terminal superfamily domain.; putative regulatory protein complement(4021352..4022074) Mycobacterium ulcerans Agy99 4553287 YP_907229.1 CDS MUL_3624 NC_008611.1 4023113 4023622 D cytoplasmic protein; hypothetical protein 4023113..4023622 Mycobacterium ulcerans Agy99 4553289 YP_907230.1 CDS MUL_3625 NC_008611.1 4023645 4024406 R in Escherichia coli RsmE methylates the N3 position of the U1498 base in 16S rRNA; cells lacking this function can grow, but are outcompeted by wild-type; SAM-dependent m(3)U1498 methyltransferase; 16S ribosomal RNA methyltransferase RsmE complement(4023645..4024406) Mycobacterium ulcerans Agy99 4553290 YP_907231.1 CDS dnaJ2 NC_008611.1 4024435 4025571 R chaperone Hsp40; co-chaperone with DnaK; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion; chaperone protein DnaJ complement(4024435..4025571) Mycobacterium ulcerans Agy99 4553291 YP_907232.1 CDS hrcA NC_008611.1 4025646 4026677 R Negative regulator of class I heat shock genes (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons; heat-inducible transcription repressor complement(4025646..4026677) Mycobacterium ulcerans Agy99 4553292 YP_907233.1 CDS MUL_3628 NC_008611.1 4026965 4027264 D cytoplasmic protein; hypothetical protein 4026965..4027264 Mycobacterium ulcerans Agy99 4553293 YP_907234.1 CDS MUL_3629 NC_008611.1 4027371 4027958 D cytoplasmic protein; function unknown but contains identity with antioxidant defence protein Ahp; hypothetical protein 4027371..4027958 Mycobacterium ulcerans Agy99 4553294 YP_907235.1 CDS MUL_3630 NC_008611.1 4027955 4028854 D cytoplasmic protein; the sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. belongs to the sigma-70 factor family, ECF subfamily.; alternative RNA polymerase sigma factor SigJ- like 4027955..4028854 Mycobacterium ulcerans Agy99 4553295 YP_907236.1 CDS mbtB NC_008611.1 4029686 4033177 R cytoplasmic protein; involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. this peptide synthase forms amide bound between the carboxylic acid of salicylate and the alpha-amino group of serine (serine/threonine ligation); phenyloxazoline synthase MbtB complement(4029686..4033177) Mycobacterium ulcerans Agy99 4553153 YP_907237.1 CDS mbtF NC_008611.1 4033213 4037751 R cytoplasmic protein; involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. activates amino acid residues that are incorporated into mycobactin (ligation); non-ribosomal peptide synthetase MbtF complement(4033213..4037751) Mycobacterium ulcerans Agy99 4553154 YP_907238.1 CDS mbtE NC_008611.1 4037748 4042760 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. probably activates the two lysine residues that are incorporated into mycobactin (lysine ligation); non-ribosomal peptide synthetase MbtE complement(4037748..4042760) Mycobacterium ulcerans Agy99 4553155 YP_907239.1 CDS MUL_3635 NC_008611.1 4043035 4043640 D cytoplasmic protein; function unknown but contains acyl-CoA N- acetyltransferase superfamily domain; hypothetical protein 4043035..4043640 Mycobacterium ulcerans Agy99 4553156 YP_907240.1 CDS MUL_3636 NC_008611.1 4043750 4044508 D cytoplasmic protein; may be required for the addition of the terminal aa in this Nrp operon; thioesterase 4043750..4044508 Mycobacterium ulcerans Agy99 4553157 YP_907241.1 CDS mbtC NC_008611.1 4044505 4045824 D cytoplasmic protein; involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins.; polyketide synthase MbtC 4044505..4045824 Mycobacterium ulcerans Agy99 4553158 YP_907242.1 CDS mbtD NC_008611.1 4045821 4048895 D cytoplasmic protein; involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins.; polyketide synthase MbtD 4045821..4048895 Mycobacterium ulcerans Agy99 4553159 YP_907243.1 CDS mbtH_1 NC_008611.1 4048900 4049115 R cytoplasmic protein; thought to be involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins.; hypothetical protein complement(4048900..4049115) Mycobacterium ulcerans Agy99 4553160 YP_907244.1 CDS mbtG NC_008611.1 4049096 4050400 R cytoplasmic protein; involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. this hydroxylase is possibly required for N-hydroxylation of the two lysine residues at some stage during mycobactin assembly [catalytic activity: L-lysine + O(2) = N6-hydroxy- L-lysine + H(2)O. no information can be found if this enzyme is NADPH dependent or independent]; lysine-N-oxygenase MbtG complement(4049096..4050400) Mycobacterium ulcerans Agy99 4553161 YP_907245.1 CDS mbtA NC_008611.1 4050636 4052249 D membrane protein; involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins (initiation step of mycobactin chain growth) activates the mycobactin ACP in two half-reactions: activates salicylic acid as acyladenylate (adenylation step) + transfers activated salicylate to the MbtA ACP as a thioester (arylation step); bifunctional enzyme MbtA: salicyl-AMP ligase (SAL-AMP ligase) + salicyl-S-ACP synthetase 4050636..4052249 Mycobacterium ulcerans Agy99 4553162 YP_907246.1 CDS mbtJ NC_008611.1 4052359 4053354 D cytoplasmic protein; involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. possibly required for N-hydroxylation of the two lysine residues at some stage during mycobactin assembly.; acetyl hydrolase MbtJ 4052359..4053354 Mycobacterium ulcerans Agy99 4553163 YP_907247.1 CDS MUL_3643 NC_008611.1 4053356 4054216 D membrane protein; short-chain membrane-associated dehydrogenase 4053356..4054216 Mycobacterium ulcerans Agy99 4553164 YP_907248.1 CDS MUL_3645 NC_008611.1 4055176 4055880 D cytoplasmic protein; function unknown. possibly involved in transcriptional mechanism.; putative transcriptional regulator 4055176..4055880 Mycobacterium ulcerans Agy99 4553166 YP_907249.1 CDS desA1_1 NC_008611.1 4055912 4056928 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; catalyzes the principal conversion of saturated fatty acids to unsaturated fatty acids. thought to convert stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons delta-9 and delta-10 of the acyl chain [catalytic activity: stearoyl-[acyl-carrier protein] + AH2 + O2 = oleoyl-[acyl-carrier protein] + a + 2 H2O]; acyl-[acyl-carrier protein] desaturase DesA1 complement(4055912..4056928) Mycobacterium ulcerans Agy99 4553167 YP_907250.1 CDS MUL_3647 NC_008611.1 4057055 4057801 R cytoplasmic protein; hypothetical protein complement(4057055..4057801) Mycobacterium ulcerans Agy99 4553168 YP_907251.1 CDS mbtI NC_008611.1 4058002 4059363 R catalyzes conversion of chorismate to salicylate, in mycobactin siderophore construction; requires Mg(2+) for function; salicylate synthase MbtI complement(4058002..4059363) Mycobacterium ulcerans Agy99 4553169 YP_907252.1 CDS MUL_3649 NC_008611.1 4059752 4059961 D cytoplasmic protein; hypothetical protein 4059752..4059961 Mycobacterium ulcerans Agy99 4553170 YP_907253.1 CDS MUL_3650 NC_008611.1 4060251 4061486 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; membrane permease 4060251..4061486 Mycobacterium ulcerans Agy99 4553171 YP_907254.1 CDS hemN NC_008611.1 4061498 4062670 R catalyzes the oxygen-independent formation of protoporphyrinogen-IX from coproporphyrinogen-III; coproporphyrinogen III oxidase complement(4061498..4062670) Mycobacterium ulcerans Agy99 4553114 YP_907255.1 CDS nirA_2 NC_008611.1 4063107 4064774 D cytoplasmic protein; generates nitrite from ammonia using oxidized ferredoxin [catalytic activity: ammonia + H(2)O + oh(-) + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin] can also act as a sulphite reductase. the sulfite reductases catalyze the reduction of sulfite to sulfide, one step in the biosynthesis of sulfur-containing amino acids and cofactors. this appears to be the only intact nitrite reductase in M. ulcerans.; ferredoxin-dependent nitrite/sulfite reductase NirA 4063107..4064774 Mycobacterium ulcerans Agy99 4553115 YP_907256.1 CDS cysH NC_008611.1 4064771 4065520 D catalyzes the reduction of 3'-phosphoadenylyl sulfate into sulfite; phosphoadenosine phosphosulfate reductase 4064771..4065520 Mycobacterium ulcerans Agy99 4553116 YP_907257.1 CDS MUL_3654 NC_008611.1 4065517 4066191 D membrane protein; function unknown but contains a chelatase superfamily domain. given the presence of other heme- associated CDS in this region and the presence of a chelatase superfamily domain, this CDS may encode a ferrochelatase, catalyzing the last step in HemE biosynthesis.; hypothetical protein 4065517..4066191 Mycobacterium ulcerans Agy99 4553117 YP_907258.1 CDS cysA1 NC_008611.1 4070337 4071428 R membrane protein; involved in the active transport across the membrane of multiple sulfur-containing compounds, including sulfate and thiosulfate (import) responsible for energy coupling to the transport system.; sulfate-transport ABC transporter ATP-binding protein CysA1 complement(4070337..4071428) Mycobacterium ulcerans Agy99 4553120 YP_907259.1 CDS cysW NC_008611.1 4071443 4072261 R membrane protein; involved in the active transport across the membrane of multiple sulfur-containing compounds, including sulfate and thiosulfate (import) responsible for the translocation of the substrate across the membrane.; sulfate-transport integral membrane protein ABC transporter CysW complement(4071443..4072261) Mycobacterium ulcerans Agy99 4553121 YP_907260.1 CDS cysT NC_008611.1 4072258 4073160 R membrane protein; involved in the active transport across the membrane of multiple sulfur-containing compounds, including sulfate and thiosulfate (import) responsible for the translocation of the substrate across the membrane.; sulfate-transport integral membrane protein ABC transporter CysT complement(4072258..4073160) Mycobacterium ulcerans Agy99 4553122 YP_907261.1 CDS subI NC_008611.1 4073157 4074206 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in the active transport across the membrane of multiple sulfur-containing compounds, including sulfate and thiosulfate (import); sulfate-binding lipoprotein SubI complement(4073157..4074206) Mycobacterium ulcerans Agy99 4553123 YP_907262.1 CDS MUL_3662 NC_008611.1 4074556 4074750 R cytoplasmic protein; hypothetical protein complement(4074556..4074750) Mycobacterium ulcerans Agy99 4553124 YP_907263.1 CDS MUL_3663 NC_008611.1 4074826 4076064 D cytoplasmic protein; function unknown, probably involved in cellular metabolism; hypothetical protein 4074826..4076064 Mycobacterium ulcerans Agy99 4553125 YP_907264.1 CDS MUL_3664 NC_008611.1 4076135 4078201 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; glycosyl hydrolase 4076135..4078201 Mycobacterium ulcerans Agy99 4553126 YP_907265.1 CDS lppR NC_008611.1 4078219 4078980 R secreted protein; lipoprotein LppR complement(4078219..4078980) Mycobacterium ulcerans Agy99 4553127 YP_907266.1 CDS lppR_1 NC_008611.1 4078992 4079735 R secreted protein; lipoprotein LppR complement(4078992..4079735) Mycobacterium ulcerans Agy99 4553128 YP_907267.1 CDS lepA NC_008611.1 4079738 4081477 R binds to the ribosome on the universally-conserved alpha-sarcin loop; GTP-binding protein LepA complement(4079738..4081477) Mycobacterium ulcerans Agy99 4553129 YP_907268.1 CDS MUL_3668 NC_008611.1 4081745 4082290 D cytoplasmic protein; function unknown but domain identity with plasmid maintenance toxin superfamily; hypothetical protein 4081745..4082290 Mycobacterium ulcerans Agy99 4553130 YP_907269.1 CDS MUL_3669 NC_008611.1 4082332 4082760 R cytoplasmic protein; function unknown but contains 2 Cbs domains; transcriptional regulator complement(4082332..4082760) Mycobacterium ulcerans Agy99 4553131 YP_907270.1 CDS MUL_3670 NC_008611.1 4082986 4083825 D member of metallo-beta-lactamase family; the purified enzyme from Escherichia coli forms dimeric zinc phosphodiesterase; in Bacillus subtilis this protein is a 3'-tRNA processing endoribonuclease and is essential while in Escherichia coli it is not; associates with two zinc ions; ribonuclease Z 4082986..4083825 Mycobacterium ulcerans Agy99 4553132 YP_907271.1 CDS MUL_3671 NC_008611.1 4083988 4085268 D PE_PGRS2; membrane protein; PE-PGRS family protein 4083988..4085268 Mycobacterium ulcerans Agy99 4553133 YP_907272.1 CDS MUL_3672 NC_008611.1 4085372 4086748 D PE_PGRS25; membrane protein; PE-PGRS family protein 4085372..4086748 Mycobacterium ulcerans Agy99 4553079 YP_907273.1 CDS MUL_3673 NC_008611.1 4086753 4087592 R cytoplasmic protein; function unknown but based on domain identity it may cross-link proteins through an acyl-transfer reaction between the gamma-carboxamide group of peptide-bound glutamine and the epsilon-amino group of peptide-bound lysine.; putative transglutaminase-like protein complement(4086753..4087592) Mycobacterium ulcerans Agy99 4553080 YP_907274.1 CDS MUL_3674 NC_008611.1 4087592 4088569 R cytoplasmic protein; hypothetical protein complement(4087592..4088569) Mycobacterium ulcerans Agy99 4553081 YP_907275.1 CDS MUL_3675 NC_008611.1 4088569 4090242 R cytoplasmic protein; function unknown but contains two glutathione synthetase ATP-binding superfamily domain-like sequences; hypothetical protein complement(4088569..4090242) Mycobacterium ulcerans Agy99 4553082 YP_907276.1 CDS rpsT NC_008611.1 4090381 4090644 D binds directly to the 16S rRNA and is involved in post-translational inhibition of arginine and ornithine decarboxylase; 30S ribosomal protein S20 4090381..4090644 Mycobacterium ulcerans Agy99 4553083 YP_907277.1 CDS holA NC_008611.1 4090716 4091693 R cytoplasmic protein; possible role in DNA replication. two superfamily domains detected. N-term contains P-loop nucleoside triphosphate hydrolase. C-term contains DNA polymerase III, delta subunit.; hypothetical protein complement(4090716..4091693) Mycobacterium ulcerans Agy99 4553084 YP_907278.1 CDS MUL_3678 NC_008611.1 4091787 4092833 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(4091787..4092833) Mycobacterium ulcerans Agy99 4553085 YP_907279.1 CDS comEC NC_008611.1 4093109 4094662 R membrane protein; function unknown, predicted membrane metal-binding protein; membrane metal-binding protein ComEC complement(4093109..4094662) Mycobacterium ulcerans Agy99 4553086 YP_907280.1 CDS comEA NC_008611.1 4094680 4095555 R membrane protein; function unknown but domain identity suggests ComEA. a family of DNA uptake protein and related DNA- binding proteins involved in DNA replication, recombination, and repair.; membrane protein ComEA complement(4094680..4095555) Mycobacterium ulcerans Agy99 4553087 YP_907281.1 CDS fadE17_1 NC_008611.1 4095810 4096976 D Detected in the membrane fraction by proteomics, and in the cytoplamic and extracellular matrix fractions by LCMSMS; cytoplasmic protein; function unknown, but probable involvement in lipid metabolism.; acyl-CoA dehydrogenase FadE17 4095810..4096976 Mycobacterium ulcerans Agy99 4553088 YP_907282.1 CDS fadE18_2 NC_008611.1 4096984 4098102 D cytoplasmic protein; function unknown, but supposed involvement in lipid metabolism.; acyl-CoA dehydrogenase FadE18 4096984..4098102 Mycobacterium ulcerans Agy99 4553089 YP_907283.1 CDS MUL_3683 NC_008611.1 4098358 4099458 D membrane protein; function unknown but contains FAD/NAD(P)-binding superfamily domain; hypothetical protein 4098358..4099458 Mycobacterium ulcerans Agy99 4553090 YP_907284.1 CDS MUL_3685 NC_008611.1 4101005 4102054 D cytoplasmic protein; function unknown but contains superfamily NAD(P)- binding Rossmann-fold domains; hypothetical protein 4101005..4102054 Mycobacterium ulcerans Agy99 4553092 YP_907285.1 CDS MUL_3686 NC_008611.1 4102057 4102833 D membrane protein; hypothetical protein 4102057..4102833 Mycobacterium ulcerans Agy99 4553093 YP_907286.1 CDS mmpS5_1 NC_008611.1 4103173 4103592 D membrane protein; membrane protein MmpS5 4103173..4103592 Mycobacterium ulcerans Agy99 4553094 YP_907287.1 CDS mmpL4_1 NC_008611.1 4103589 4106510 D membrane protein; function unknown. thought to be involved in lipid transport.; transmembrane transport protein MmpL 4103589..4106510 Mycobacterium ulcerans Agy99 4553050 YP_907288.1 CDS MUL_3689 NC_008611.1 4106546 4107391 R cytoplasmic protein; function unknown, contains DegV-like superfamily domain; hypothetical protein complement(4106546..4107391) Mycobacterium ulcerans Agy99 4553051 YP_907289.1 CDS MUL_3690 NC_008611.1 4107395 4108150 R cytoplasmic protein; function unknown but contains esterase/acetylhydrolase superfamily domain; hypothetical protein complement(4107395..4108150) Mycobacterium ulcerans Agy99 4553052 YP_907290.1 CDS gpm_1 NC_008611.1 4108140 4108814 R cytoplasmic protein; involved in glycolysis [catalytic activity: 2- phosphoglycerate + 2,3-diphosphoglycerate = 3- phosphoglycerate + 2,3-diphosphoglycerate]; phosphoglycerate mutase complement(4108140..4108814) Mycobacterium ulcerans Agy99 4553053 YP_907291.1 CDS nadD NC_008611.1 4108811 4109845 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in NAD biosynthesis; catalyzes the reversible adenylation of nicotinate mononucleotide [catalytic activity: ATP + nicotinate ribonucleotide = diphosphate + deamido-NAD(+)] this CDS is a combination of NadD translationally fused at the C-term with an orthologue of Rv2420c. this coupling is not seen in M. marinum nor in other mycobacteria although in other species Rv2420c is immediately d/s of NadD. probably caused by mutational loss of a stop codon.; bifunctional nicotinate-nucleotide adenylyltransferase NadD/hypothetical protein complement(4108811..4109845) Mycobacterium ulcerans Agy99 4553054 YP_907292.1 CDS MUL_3694 NC_008611.1 4111129 4112568 R cytoplasmic protein; function unknown but contains a von willebrand factor type a conserved domain; hypothetical protein complement(4111129..4112568) Mycobacterium ulcerans Agy99 4553056 YP_907293.1 CDS MUL_3695 NC_008611.1 4112582 4113457 R Also detected in the membrane fraction by proteomics; cytoplasmic protein; function unknown but contains aaa ATPase domain containing von willebrand factor type a (vWA) domain; hypothetical protein complement(4112582..4113457) Mycobacterium ulcerans Agy99 4553057 YP_907294.1 CDS proA NC_008611.1 4113491 4114762 R Catalyzes the phosphorylation of L-glutamate during the proline biosynthesis pathway; gamma-glutamyl phosphate reductase complement(4113491..4114762) Mycobacterium ulcerans Agy99 4553058 YP_907295.1 CDS MUL_3697 NC_008611.1 4114855 4116249 R membrane protein; hypothetical protein complement(4114855..4116249) Mycobacterium ulcerans Agy99 4553059 YP_907296.1 CDS MUL_3698 NC_008611.1 4116246 4118357 R membrane protein; generates 3,'5'-cyclic (A/G)mp and diphosphate (or pyrophosphate) from (A/G)tp.; adenylate or guanylate cyclase complement(4116246..4118357) Mycobacterium ulcerans Agy99 4553060 YP_907297.1 CDS MUL_3699 NC_008611.1 4118604 4120004 R cytoplasmic protein; function unknown but domain identity suggests that it may be a mechanosensitive (ms) ion channel protein with a C-term putative cyclic nucleotide (camp or cGMP) binding domain.; hypothetical protein complement(4118604..4120004) Mycobacterium ulcerans Agy99 4553061 YP_907298.1 CDS MUL_3702 NC_008611.1 4122738 4124072 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 4122738..4124072 Mycobacterium ulcerans Agy99 4553063 YP_907299.1 CDS MUL_3703 NC_008611.1 4124507 4125553 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 4124507..4125553 Mycobacterium ulcerans Agy99 4553064 YP_907300.1 CDS MUL_3704 NC_008611.1 4125622 4126998 D PE_PGRS33_1; membrane protein; function unknown. seems to influence both cell surface interactions among mycobacteria and the interactions of bacteria with macrophages; PE-PGRS family protein 4125622..4126998 Mycobacterium ulcerans Agy99 4553065 YP_907301.1 CDS nadE NC_008611.1 4127111 4129153 R catalyzes the formation of nicotinamide adenine dinucleotide (NAD) from nicotinic acid adenine dinucleotide (NAAD) using either ammonia or glutamine as the amide donor and ATP; ammonia-utilizing enzymes include the ones from Bacillus and Escherichia coli while glutamine-utilizing enzymes include the Mycobacterial one; forms homodimers; NAD synthetase complement(4127111..4129153) Mycobacterium ulcerans Agy99 4553066 YP_907302.1 CDS MUL_3706 NC_008611.1 4129463 4130314 D cytoplasmic protein; function unknown, involved in transcriptional mechanism; Sir2-like regulatory protein 4129463..4130314 Mycobacterium ulcerans Agy99 4553000 YP_907303.1 CDS MUL_3708 NC_008611.1 4131788 4132426 D cytoplasmic protein; function unknown. possibly involved in transcriptional mechanism.; AcrR family transcriptional regulator 4131788..4132426 Mycobacterium ulcerans Agy99 4553002 YP_907304.1 CDS proB NC_008611.1 4137065 4138171 R catalyzes the formation of glutamate 5-phosphate from glutamate in proline biosynthesis; gamma-glutamyl kinase complement(4137065..4138171) Mycobacterium ulcerans Agy99 4553004 YP_907305.1 CDS obgE NC_008611.1 4138171 4139610 R essential GTPase; exhibits high exchange rate for GTP/GDP; associates with 50S ribosomal subunit; involved in regulation of chromosomal replication; GTPase ObgE complement(4138171..4139610) Mycobacterium ulcerans Agy99 4553005 YP_907306.1 CDS rpmA NC_008611.1 4139715 4139981 R involved in the peptidyltransferase reaction during translation; 50S ribosomal protein L27 complement(4139715..4139981) Mycobacterium ulcerans Agy99 4553006 YP_907307.1 CDS rplU NC_008611.1 4139999 4140310 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; involved in translation mechanisms.; 50S ribosomal protein L21 complement(4139999..4140310) Mycobacterium ulcerans Agy99 4553007 YP_907308.1 CDS rne NC_008611.1 4140583 4143549 R cytoplasmic protein; thought to be involved in several cellular process.; ribonuclease E Rne complement(4140583..4143549) Mycobacterium ulcerans Agy99 4553008 YP_907309.1 CDS ndk NC_008611.1 4143876 4144286 R catalyzes the formation of nucleoside triphosphate from ATP and nucleoside diphosphate; nucleoside diphosphate kinase complement(4143876..4144286) Mycobacterium ulcerans Agy99 4553009 YP_907310.1 CDS MUL_3717 NC_008611.1 4144350 4144754 R membrane protein; hypothetical protein complement(4144350..4144754) Mycobacterium ulcerans Agy99 4553010 YP_907311.1 CDS folC NC_008611.1 4144751 4146205 R cytoplasmic protein; conversion of folates to polyglutamate derivatives. bacteria require folate for the biosynthesis of glycine, methionine, formyl-met-tRNA, thymidylates, purines, and pantothenate [catalytic activity: ATP + {tetrahydrofolyl- [Glu]}(N) + L-glutamate = ADP + phosphate + {tetrahydrofolyl-[Glu]}(n+1)]; folylpolyglutamate synthase protein FolC complement(4144751..4146205) Mycobacterium ulcerans Agy99 4553011 YP_907312.1 CDS valS NC_008611.1 4146202 4148862 R valine--tRNA ligase; ValRS; converts valine ATP and tRNA(Val) to AMP PPi and valyl-tRNA(Val); class-I aminoacyl-tRNA synthetase type 1 subfamily; has a posttransfer editing process to hydrolyze mischarged Thr-tRNA(Val) which is done by the editing domain; valyl-tRNA synthetase complement(4146202..4148862) Mycobacterium ulcerans Agy99 4553012 YP_907313.1 CDS MUL_3720 NC_008611.1 4148937 4149560 R Detected in the cytoplasm and in the extracellular matrix by proteomics.; cytoplasmic protein; function unknown but N-term contains a bulb-type mannose-specific lectin and C-term contains a LysM, a putative peptidoglycan-binding domain.; hypothetical protein complement(4148937..4149560) Mycobacterium ulcerans Agy99 4553013 YP_907314.1 CDS MUL_3721 NC_008611.1 4149644 4150249 R membrane protein; hypothetical protein complement(4149644..4150249) Mycobacterium ulcerans Agy99 4553014 YP_907315.1 CDS MUL_3722 NC_008611.1 4150348 4151604 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS) Also Detected in the extracellular matrix and the cytoplasmic fractions by proteomics.; membrane protein; function unknown but contains potential saccharopine-like dehydrogenase domain; hypothetical protein complement(4150348..4151604) Mycobacterium ulcerans Agy99 4553015 YP_907316.1 CDS rpfE NC_008611.1 4151771 4152415 R secreted protein; thought to promote the resuscitation and growth of dormant, nongrowing cells. could also stimulate the growth of several other high G+C Gram+ organisms, E.G. mycobacterium avium, mycobacterium bovis (BCG), mycobacterium kansasii, mycobacterium smegmatis.; resuscitation-promoting factor RpfE complement(4151771..4152415) Mycobacterium ulcerans Agy99 4552932 YP_907317.1 CDS mobA NC_008611.1 4152947 4153552 R MobA; links a guanosine 5'-phosphate to molydopterin to form molybdopterin guanine dinucleotide; involved in molybdenum cofactor biosynthesis; molybdopterin-guanine dinucleotide biosynthesis protein A complement(4152947..4153552) Mycobacterium ulcerans Agy99 4552933 YP_907318.1 CDS porB NC_008611.1 4153555 4154646 R catalyzes the coenzyme A dependent formation of succinyl-CoA from 2-oxoglutarate and ferredoxin; 2-oxoglutarate ferredoxin oxidoreductase subunit beta complement(4153555..4154646) Mycobacterium ulcerans Agy99 4552934 YP_907319.1 CDS porA NC_008611.1 4154695 4156656 R Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); cytoplasmic protein; function unknown, probably involved in cellular metabolism.; pyruvate:ferredoxin oxidoreductase, PorA, alpha subunit complement(4154695..4156656) Mycobacterium ulcerans Agy99 4552935 YP_907320.1 CDS MUL_3727 NC_008611.1 4157002 4158300 R membrane protein; thought to be involved in transport of undeterminated substrate (possibly sugar) across the membrane. responsible for the translocation of the substrate across the membrane.; integral membrane transport protein complement(4157002..4158300) Mycobacterium ulcerans Agy99 4552936 YP_907321.1 CDS clpX NC_008611.1 4158310 4159590 R binds and unfolds substrates as part of the ClpXP protease; ATP-dependent protease ATP-binding subunit ClpX complement(4158310..4159590) Mycobacterium ulcerans Agy99 4552937 YP_907322.1 CDS MUL_3729 NC_008611.1 4160226 4161890 D membrane protein; thought to be involved in a transport system across the membrane (perhaps drug transport): responsible for the translocation of the substrate across the membrane.; integral membrane transport protein 4160226..4161890 Mycobacterium ulcerans Agy99 4552938 YP_907323.1 CDS clpP2 NC_008611.1 4161947 4162582 R hydrolyzes proteins to small peptides; with the ATPase subunits ClpA or ClpX, ClpP degrades specific substrates; ATP-dependent Clp protease proteolytic subunit complement(4161947..4162582) Mycobacterium ulcerans Agy99 4552939 YP_907324.1 CDS clpP NC_008611.1 4162579 4163175 R hydrolyzes proteins to small peptides; with the ATPase subunits ClpA or ClpX, ClpP degrades specific substrates; ATP-dependent Clp protease proteolytic subunit complement(4162579..4163175) Mycobacterium ulcerans Agy99 4552940 YP_907325.1 CDS tig NC_008611.1 4163298 4164752 R Tig; RopA; peptidyl-prolyl cis/trans isomerase; promotes folding of newly synthesized proteins; binds ribosomal 50S subunit; forms a homodimer; trigger factor complement(4163298..4164752) Mycobacterium ulcerans Agy99 4552941 YP_907326.1 CDS lipP NC_008611.1 4165218 4166318 D cytoplasmic protein; function unknown, lipolytic enzyme involved in cellular metabolism.; esterase/lipase LipP 4165218..4166318 Mycobacterium ulcerans Agy99 4552944 YP_907327.1 CDS MUL_3736 NC_008611.1 4166402 4166698 R secreted protein; hypothetical protein complement(4166402..4166698) Mycobacterium ulcerans Agy99 4552945 YP_907328.1 CDS MUL_3737 NC_008611.1 4166820 4167626 R cytoplasmic protein; hydrolyses DNA (this enzyme may play a significant role in processes leading to recovery from mutagenesis and/or cell death by alkylating agents); formamidopyrimidine-DNA glycosylase complement(4166820..4167626) Mycobacterium ulcerans Agy99 4552946 YP_907329.1 CDS rpiB NC_008611.1 4167635 4168114 R catalyzes the interconversion of ribose 5-phosphate to ribulose 5-phosphate; enzyme from E. coli shows allose 6-phosphate isomerase activity; ribose-5-phosphate isomerase B complement(4167635..4168114) Mycobacterium ulcerans Agy99 4549706 YP_907330.1 CDS MUL_3741 NC_008611.1 4170425 4171621 R PE_PGRS59; membrane protein; N-term PE-PGRS family protein complement(4170425..4171621) Mycobacterium ulcerans Agy99 4549709 YP_907331.1 CDS pepN NC_008611.1 4171897 4174476 D Also detected in the extracellular matrix by proteomics. Also detected in the membrane fraction by proteomics.; cytoplasmic protein; aminopeptidase with broad substrate specificity to several peptides (could preferentially cleave leucine, arginine and lysine in peptide-bond-containing substrates); aminopeptidase N PepN 4171897..4174476 Mycobacterium ulcerans Agy99 4549710 YP_907332.1 CDS MUL_3743 NC_008611.1 4174569 4175036 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (LC-MS/MS); cytoplasmic protein; hypothetical protein complement(4174569..4175036) Mycobacterium ulcerans Agy99 4549711 YP_907333.1 CDS MUL_3744 NC_008611.1 4175023 4175256 R cytoplasmic protein; hypothetical protein complement(4175023..4175256) Mycobacterium ulcerans Agy99 4549712 YP_907334.1 CDS MUL_3745 NC_008611.1 4175358 4175999 R cytoplasmic protein; function unknown but contains McrA, restriction endonuclease domain; hypothetical protein complement(4175358..4175999) Mycobacterium ulcerans Agy99 4549713 YP_907335.1 CDS glbO NC_008611.1 4176153 4176539 D cytoplasmic protein; oxygen carrier, involved in oxygen transport.; globin (oxygen-binding protein) GlbO 4176153..4176539 Mycobacterium ulcerans Agy99 4549714 YP_907336.1 CDS choD_1 NC_008611.1 4179915 4181654 R cytoplasmic protein; involved in cholesterol metabolism [catalytic activity: cholesterol + O(2) = cholest-4-en-3-one + H(2)O(2)]; cholesterol oxidase ChoD complement(4179915..4181654) Mycobacterium ulcerans Agy99 4549717 YP_907337.1 CDS MUL_3752 NC_008611.1 4186262 4186966 D cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 4186262..4186966 Mycobacterium ulcerans Agy99 4549720 YP_907338.1 CDS MUL_3753 NC_008611.1 4186991 4187308 D cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 4186991..4187308 Mycobacterium ulcerans Agy99 4549721 YP_907339.1 CDS MUL_3754 NC_008611.1 4187371 4188033 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(4187371..4188033) Mycobacterium ulcerans Agy99 4549722 YP_907340.1 CDS MUL_3755 NC_008611.1 4188030 4188446 R cytoplasmic protein; function unknown but some identity with thioesterase domains; hypothetical protein complement(4188030..4188446) Mycobacterium ulcerans Agy99 4549723 YP_907341.1 CDS MUL_3757 NC_008611.1 4193462 4195138 R ChvD; in Agrobacterium tumefaciens, mutations in both Walker boxes were found to affect virulence; putative ABC transporter ATP-binding protein complement(4193462..4195138) Mycobacterium ulcerans Agy99 4549725 YP_907342.1 CDS MUL_3758 NC_008611.1 4195206 4195703 R cytoplasmic protein; function unknown but may play a role in DNA replication, recombination, and repair; hypothetical protein complement(4195206..4195703) Mycobacterium ulcerans Agy99 4549726 YP_907343.1 CDS plsC NC_008611.1 4201067 4202992 R membrane protein; N-terminus: could be generate serine and phosphate from phosphoserine; may catalyze the last step in the biosynthesis of serine from carbohydrates (the reaction mechanism could be proceed via the formation of a phosphoryl-enzyme intermediates) [catalytic activity 1: phosphoserine + H(2)O = serine + phosphate] mid-section: involved in phospholipid biosynthesis (at the second step); converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating acyl moiety at the 2 position [catalytic activity 2: acyl-CoA + 1-acyl-SN- glycerol 3-phosphate = CoA + 1,2-diacyl-SN-glycerol 3- phosphate] c-terminus: contains tola protein domain homology. may serve to anchor this protein in the cm.; bifunctional transmembrane phospholipid biosynthesis enzyme PlsC complement(4201067..4202992) Mycobacterium ulcerans Agy99 4549730 YP_907344.1 CDS MUL_3765 NC_008611.1 4202989 4204491 R membrane protein; function unknown but N-term contains a CoA- dependanant acyltransferase domain.; hypothetical protein complement(4202989..4204491) Mycobacterium ulcerans Agy99 4549731 YP_907345.1 CDS lipQ NC_008611.1 4204703 4205956 R cytoplasmic protein; function unknown, lipolytic enzyme involved in cellular metabolism.; carboxylesterase LipQ complement(4204703..4205956) Mycobacterium ulcerans Agy99 4549732 YP_907346.1 CDS MUL_3769 NC_008611.1 4207301 4207612 R cytoplasmic protein; hypothetical protein complement(4207301..4207612) Mycobacterium ulcerans Agy99 4549735 YP_907347.1 CDS cobQ1 NC_008611.1 4207815 4209290 R catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation; cobyric acid synthase complement(4207815..4209290) Mycobacterium ulcerans Agy99 4549736 YP_907348.1 CDS MUL_3771 NC_008611.1 4210009 4210419 D secreted protein; hypothetical protein 4210009..4210419 Mycobacterium ulcerans Agy99 4549737 YP_907349.1 CDS mmaA5 NC_008611.1 4210438 4211307 R Detected in the membrane fraction by proteomics.; membrane protein; involved in mycolic acids modification. catalyzes unusual S-adenosyl-methionine-dependent transformation of a cis-olefin mycolic acid into a secondary alcohol. catalyzes introduction of a hydroxyl group at the distal position on mycolic acid chains to produce the hydroxyl mycolate. mycolic acids represent a major constituent of the mycobacterial cell wall complex. methyl transfer results in formation of a secondary hydroxy group with an adjacent methyl branch; olefinic mycolic acid methyl transferase.; methoxy mycolic acid synthase 5 MmaA5 complement(4210438..4211307) Mycobacterium ulcerans Agy99 4549738 YP_907350.1 CDS pdhC NC_008611.1 4211626 4212795 R cytoplasmic protein; involved in energy metabolism. the pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA & CO(2) it contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) & lipoamide dehydrogenase (E3) [catalytic activity: acetyl-CoA + dihydrolipoamide = CoA + S- acetyldihydrolipoamide]; branched-chain alpha-keto acid dehydrogenase subunit E2 complement(4211626..4212795) Mycobacterium ulcerans Agy99 4549739 YP_907351.1 CDS pdhB NC_008611.1 4212792 4213838 R cytoplasmic protein; involved in energy metabolism. the pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA & CO(2) it contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) & lipoamide dehydrogenase (E3) [catalytic activity: acetyl-CoA + dihydrolipoamide = CoA + S- acetyldihydrolipoamide]; pyruvate dehydrogenase E1 component subunit PdhB complement(4212792..4213838) Mycobacterium ulcerans Agy99 4549740 YP_907352.1 CDS pdhA NC_008611.1 4213849 4214916 R cytoplasmic protein; involved in energy metabolism. the pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA & CO(2) it contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) & lipoamide dehydrogenase (E3) [catalytic activity: acetyl-CoA + dihydrolipoamide = CoA + S- acetyldihydrolipoamide]; pyruvate dehydrogenase E1 component subunit PdhA complement(4213849..4214916) Mycobacterium ulcerans Agy99 4553465 YP_907353.1 CDS citE NC_008611.1 4215281 4216102 R cytoplasmic protein; interconversion of acetate and oxaloacetate from citrate [catalytic activity: citrate = acetate + oxaloacetate]; citrate (pro-3S)-lyase subunit beta complement(4215281..4216102) Mycobacterium ulcerans Agy99 4553466 YP_907354.1 CDS MUL_3777 NC_008611.1 4216099 4216605 R Detected in the cytoplamic fraction by LCMSMS; cytoplasmic protein; function unknown but contains a central MaoC, acyl dehydratase domain; hypothetical protein complement(4216099..4216605) Mycobacterium ulcerans Agy99 4553467 YP_907355.1 CDS fadE19 NC_008611.1 4216602 4217768 R cytoplasmic protein; function unknown, but seems involved in metabolism of small branched-chain fatty acids and macrolide antibiotic production. catalyses the alpha, beta- dehydrogenetion of acyl-CoA esters and transfer electrons to ETF, the electron transfer protein.; acyl-CoA dehydrogenase FadE19 complement(4216602..4217768) Mycobacterium ulcerans Agy99 4553468 YP_907356.1 CDS accA1 NC_008611.1 4217775 4219784 R cytoplasmic protein; this protein carries two functions: biotin carboxyl carrier protein and biotin carboxyltransferase. involved in the first step of long-chain fatty acid synthesis [catalytic activity: ATP + biotin-carboxyl-carrier protein + CO(2) = ADP + phosphate + carboxybiotin-carboxyl-carrier protein]; acetyl-/propionyl-coenzyme a carboxylase alpha chain AccA1 complement(4217775..4219784) Mycobacterium ulcerans Agy99 4553469 YP_907357.1 CDS scoB NC_008611.1 4221361 4222008 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; involved in various degradation and synthesis [catalytic activity: succinyl-CoA + a 3-oxo acid = succinate + a 3-oxo-acyl-CoA]; succinyl-CoA:3-ketoacid-coenzyme a transferase (beta subunit) ScoB complement(4221361..4222008) Mycobacterium ulcerans Agy99 4553471 YP_907358.1 CDS scoA NC_008611.1 4222164 4222910 R cytoplasmic protein; involved in fatty acid degradation/synthesis [catalytic activity: succinyl-CoA + a 3-oxo acid = succinate + a 3-oxo-acyl-CoA]; succinyl-CoA:3-ketoacid-coenzyme a transferase (alpha subunit) ScoA complement(4222164..4222910) Mycobacterium ulcerans Agy99 4553472 YP_907359.1 CDS MUL_3784 NC_008611.1 4223284 4224330 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(4223284..4224330) Mycobacterium ulcerans Agy99 4553474 YP_907360.1 CDS MUL_3785 NC_008611.1 4226155 4226790 D cytoplasmic protein; involved in transcriptional mechanism.; putative regulatory protein 4226155..4226790 Mycobacterium ulcerans Agy99 4553475 YP_907361.1 CDS MUL_3786 NC_008611.1 4227021 4227812 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; proline rich membrane protein 4227021..4227812 Mycobacterium ulcerans Agy99 4553476 YP_907362.1 CDS MUL_3787 NC_008611.1 4227809 4229137 R membrane protein; integral membrane leucine and alanine rich protein complement(4227809..4229137) Mycobacterium ulcerans Agy99 4553477 YP_907363.1 CDS MUL_3788 NC_008611.1 4229233 4230039 D cytoplasmic protein; function unknown, possibly involved in cellular metabolism.; short-chain type dehydrogenase/reductase 4229233..4230039 Mycobacterium ulcerans Agy99 4553478 YP_907364.1 CDS orn NC_008611.1 4231764 4232411 D 3'-5' exoribonuclease specific for small oligoribonuclotides; oligoribonuclease 4231764..4232411 Mycobacterium ulcerans Agy99 4554333 YP_907365.1 CDS MUL_3792 NC_008611.1 4232597 4233298 R membrane protein; hypothetical protein complement(4232597..4233298) Mycobacterium ulcerans Agy99 4554335 YP_907366.1 CDS MUL_3793 NC_008611.1 4233262 4234050 R cytoplasmic protein; involved in transcriptional mechanism.; putative regulatory protein complement(4233262..4234050) Mycobacterium ulcerans Agy99 4554336 YP_907367.1 CDS yrbE5A NC_008611.1 4234348 4235217 D membrane protein; function unknown, YrbE family protein that is similar to Ttg2B-like ABC-type transport system involved in resistance to organic solvents, permease component.; YrbE family protein, YrbE5A 4234348..4235217 Mycobacterium ulcerans Agy99 4554337 YP_907368.1 CDS mce5A NC_008611.1 4236107 4237639 D membrane protein; this family of proteins contains the Mce (mycobacterial cell entry) proteins from mycobacterium tuberculosis. the archetype (Rv0169), was isolated as being necessary for colonisation of, and survival within, the macrophage. this family contains proteins of unknown function from other bacteria.; Mce protein, Mce5A 4236107..4237639 Mycobacterium ulcerans Agy99 4554339 YP_907369.1 CDS mce5B NC_008611.1 4237636 4238691 D membrane protein; this family of proteins contains the Mce (mycobacterial cell entry) proteins from mycobacterium tuberculosis. the archetype (Rv0169), was isolated as being necessary for colonisation of, and survival within, the macrophage. this family contains proteins of unknown function from other bacteria.; Mce family protein, Mce5B 4237636..4238691 Mycobacterium ulcerans Agy99 4554340 YP_907370.1 CDS mce5C NC_008611.1 4238684 4239739 D membrane protein; this family of proteins contains the Mce (mycobacterial cell entry) proteins from mycobacterium tuberculosis. the archetype (Rv0169), was isolated as being necessary for colonisation of, and survival within, the macrophage. this family contains proteins of unknown function from other bacteria.; Mce family protein, Mce5C 4238684..4239739 Mycobacterium ulcerans Agy99 4554341 YP_907371.1 CDS mce5E NC_008611.1 4240851 4242056 D membrane protein; this family of proteins contains the Mce (mycobacterial cell entry) proteins from mycobacterium tuberculosis. the archetype (Rv0169), was isolated as being necessary for colonisation of, and survival within, the macrophage. this family contains proteins of unknown function from other bacteria.; Mce family protein, Mce5E 4240851..4242056 Mycobacterium ulcerans Agy99 4554343 YP_907372.1 CDS mce5F NC_008611.1 4242053 4243303 D membrane protein; this family of proteins contains the Mce (mycobacterial cell entry) proteins from mycobacterium tuberculosis. the archetype (Rv0169), was isolated as being necessary for colonisation of, and survival within, the macrophage. this family contains proteins of unknown function from other bacteria.; Mce family protein Mce5F 4242053..4243303 Mycobacterium ulcerans Agy99 4554344 YP_907373.1 CDS MUL_3802 NC_008611.1 4243331 4244023 D cytoplasmic protein; hypothetical protein 4243331..4244023 Mycobacterium ulcerans Agy99 4554345 YP_907374.1 CDS MUL_3803 NC_008611.1 4244042 4244821 D secreted protein; hypothetical protein 4244042..4244821 Mycobacterium ulcerans Agy99 4554346 YP_907375.1 CDS lppS NC_008611.1 4244823 4246025 R secreted protein; lipoprotein LppS complement(4244823..4246025) Mycobacterium ulcerans Agy99 4554347 YP_907376.1 CDS MUL_3805 NC_008611.1 4246026 4246163 D secreted protein; hypothetical protein 4246026..4246163 Mycobacterium ulcerans Agy99 4554348 YP_907377.1 CDS MUL_3808 NC_008611.1 4246971 4247855 D cytoplasmic protein; function unknown but domain identity suggests PlsC, phosphate acyltransferase. these enzymes function in phospholipid biosynthesis and have either glycerolphosphate, 1-acylglycerolphosphate, or 2- acylglycerolphosphoethanolamine acyltransferase activities.; hypothetical protein 4246971..4247855 Mycobacterium ulcerans Agy99 4554252 YP_907378.1 CDS MUL_3809 NC_008611.1 4248048 4249787 D membrane protein; hypothetical protein 4248048..4249787 Mycobacterium ulcerans Agy99 4554253 YP_907379.1 CDS MUL_3810 NC_008611.1 4249899 4250126 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(4249899..4250126) Mycobacterium ulcerans Agy99 4554254 YP_907380.1 CDS bcp NC_008611.1 4250234 4250707 D Detected in the cytoplasmic and membrane fractiond and in the secreted proteins by 2D-LC-MS/MS.; cytoplasmic protein; putative antioxidant protein.; bacterioferritin comigratory protein Bcp 4250234..4250707 Mycobacterium ulcerans Agy99 4554255 YP_907381.1 CDS argE NC_008611.1 4250708 4252033 R cytoplasmic protein; deacetylates acetylornithine and hydrolyses N- acetylmethionine.; hypothetical protein complement(4250708..4252033) Mycobacterium ulcerans Agy99 4554256 YP_907382.1 CDS MUL_3814 NC_008611.1 4253214 4253870 D cytoplasmic protein; regulator part of the two component regulatory system.; two component transcriptional regulator 4253214..4253870 Mycobacterium ulcerans Agy99 4554258 YP_907383.1 CDS acpS NC_008611.1 4255894 4256286 R Catalyzes the formation of holo-ACP, which mediates the essential transfer of acyl fatty acid intermediates during the biosynthesis of fatty acids and lipids; 4'-phosphopantetheinyl transferase complement(4255894..4256286) Mycobacterium ulcerans Agy99 4554261 YP_907384.1 CDS fas NC_008611.1 4256315 4265533 R Detected in the membrane fraction by proteomics (LC- MS/MS) Also detected in the cytoplasm.; membrane protein; involved in lipid metabolism. fatty acid synthase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH; fatty acid synthase Fas complement(4256315..4265533) Mycobacterium ulcerans Agy99 4554262 YP_907385.1 CDS MUL_3819 NC_008611.1 4266041 4266763 R secreted protein; hypothetical protein complement(4266041..4266763) Mycobacterium ulcerans Agy99 4554263 YP_907386.1 CDS MUL_3820 NC_008611.1 4267005 4268147 D may act to bind either a ubiquinol or plastoquinol anion, transferring an electron to the 2Fe-2S cluster, then releasing the electron to a cytochrome C or cytochrome F haem iron.; ring-hydroxylating dioxygenase, large terminal subunit 4267005..4268147 Mycobacterium ulcerans Agy99 4554264 YP_907387.1 CDS maoC NC_008611.1 4269736 4270182 R cytoplasmic protein; function unknown role in lipid metabolism; contains a thioesterase/thiol ester dehydratase-isomerase superfamily domain; acyl dehydratase MaoC complement(4269736..4270182) Mycobacterium ulcerans Agy99 4554266 YP_907388.1 CDS MUL_3823 NC_008611.1 4270179 4270697 R cytoplasmic protein; function unknown but like d/s ORF it contains a thioesterase/thiol ester dehydrase-isomerase superfmaily domain.; hypothetical protein complement(4270179..4270697) Mycobacterium ulcerans Agy99 4554267 YP_907389.1 CDS MUL_3824 NC_008611.1 4270721 4271164 R Also detected in the extracellular matrix by proteomics.; cytoplasmic protein; hypothetical protein complement(4270721..4271164) Mycobacterium ulcerans Agy99 4554268 YP_907390.1 CDS MUL_3826 NC_008611.1 4273115 4274791 D membrane protein; function unknown, possibly involved in transport of drug across the membrane. contains AraJ, arabinose efflux permease domain identity.; hypothetical protein 4273115..4274791 Mycobacterium ulcerans Agy99 4554145 YP_907391.1 CDS MUL_3827 NC_008611.1 4275071 4276117 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(4275071..4276117) Mycobacterium ulcerans Agy99 4554146 YP_907392.1 CDS MUL_3828 NC_008611.1 4276335 4276601 R cytoplasmic protein; function unknown but acetyl-CoA synthetase-like superfamily domain detected.; hypothetical protein complement(4276335..4276601) Mycobacterium ulcerans Agy99 4554147 YP_907393.1 CDS MUL_3829 NC_008611.1 4276666 4277793 R cytoplasmic protein; function unknown, probably involved in cellular metabolism. contains domain identity to phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases.; oxidoreductase complement(4276666..4277793) Mycobacterium ulcerans Agy99 4554148 YP_907394.1 CDS fdxD_1 NC_008611.1 4278379 4278570 R cytoplasmic protein; ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.; ferredoxin FdxD complement(4278379..4278570) Mycobacterium ulcerans Agy99 4554149 YP_907395.1 CDS MUL_3832 NC_008611.1 4281091 4281912 D cytoplasmic protein; involved in transcriptional mechanism.; FadR family transcriptional regulator 4281091..4281912 Mycobacterium ulcerans Agy99 4554151 YP_907396.1 CDS MUL_3833 NC_008611.1 4282283 4283617 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 4282283..4283617 Mycobacterium ulcerans Agy99 4554152 YP_907397.1 CDS MUL_3834 NC_008611.1 4284052 4285098 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 4284052..4285098 Mycobacterium ulcerans Agy99 4554153 YP_907398.1 CDS MUL_3837 NC_008611.1 4288220 4289266 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(4288220..4289266) Mycobacterium ulcerans Agy99 4554156 YP_907399.1 CDS MUL_3839 NC_008611.1 4291244 4292092 R cytoplasmic protein; coenzyme F420-dependent oxidoreductase complement(4291244..4292092) Mycobacterium ulcerans Agy99 4554128 YP_907400.1 CDS MUL_3840 NC_008611.1 4292182 4293231 D cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase 4292182..4293231 Mycobacterium ulcerans Agy99 4554129 YP_907401.1 CDS fadE17_2 NC_008611.1 4294340 4295497 D cytoplasmic protein; function unknown, but supposed involvement in lipid degradation.; acyl-CoA dehydrogenase FadE17 4294340..4295497 Mycobacterium ulcerans Agy99 4554131 YP_907402.1 CDS MUL_3843 NC_008611.1 4295494 4296429 D cytoplasmic protein; function unknown role in lipid metabolism; acyl-CoA dehydrogenase 4295494..4296429 Mycobacterium ulcerans Agy99 4554132 YP_907403.1 CDS MUL_3844 NC_008611.1 4296426 4297919 D cytoplasmic protein; function unknown, but involved in lipid degradation.; fatty-acid-CoA ligase 4296426..4297919 Mycobacterium ulcerans Agy99 4554133 YP_907404.1 CDS MUL_3846 NC_008611.1 4300217 4300585 D secreted protein; hypothetical protein 4300217..4300585 Mycobacterium ulcerans Agy99 4554135 YP_907405.1 CDS MUL_3847 NC_008611.1 4300805 4301281 D membrane protein; function unknown but contains a ricin-type beta- trefoil domain.; hypothetical protein 4300805..4301281 Mycobacterium ulcerans Agy99 4554136 YP_907406.1 CDS MUL_3848 NC_008611.1 4301448 4302467 D cytoplasmic protein; function unknown. possibly involved in transcriptional mechanism.; putative transcriptional regulatory protein 4301448..4302467 Mycobacterium ulcerans Agy99 4554137 YP_907407.1 CDS MUL_3849 NC_008611.1 4302539 4303744 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS; cytoplasmic protein; metal-dependent hydrolase 4302539..4303744 Mycobacterium ulcerans Agy99 4554138 YP_907408.1 CDS rsbU NC_008611.1 4307110 4309143 D cytoplasmic protein; may be involved in regulation of SigG; regulator of sigma subunit, anti-anti-sigma factor RsbU 4307110..4309143 Mycobacterium ulcerans Agy99 4554141 YP_907409.1 CDS MUL_3854 NC_008611.1 4309156 4310391 R cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase complement(4309156..4310391) Mycobacterium ulcerans Agy99 4554142 YP_907410.1 CDS MUL_3858 NC_008611.1 4313093 4313902 D hypothetical protein 4313093..4313902 Mycobacterium ulcerans Agy99 4553893 YP_907411.1 CDS MUL_3859 NC_008611.1 4313899 4314561 D cytoplasmic protein; hypothetical protein 4313899..4314561 Mycobacterium ulcerans Agy99 4553894 YP_907412.1 CDS cyp187A4 NC_008611.1 4314847 4316073 D cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.; cytochrome P450 187A4 Cyp187A4 4314847..4316073 Mycobacterium ulcerans Agy99 4553895 YP_907413.1 CDS adhB_1 NC_008611.1 4316070 4317224 D cytoplasmic protein; thought to catalyze the reversible oxidation of ethanol to acetaldehyde with the concomitant reduction of NAD. probably acts on primary or secondary alcohols or hemiacetals [catalytic activity: an alcohol + NAD+ = an aldehyde or ketone + NADH]; zinc-containing alcohol dehydrogenase NAD dependent AdhB 4316070..4317224 Mycobacterium ulcerans Agy99 4553896 YP_907414.1 CDS MUL_3862 NC_008611.1 4317167 4318081 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; short chain dehydrogenase complement(4317167..4318081) Mycobacterium ulcerans Agy99 4553897 YP_907415.1 CDS cyp108B4 NC_008611.1 4318186 4319418 R cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.; cytochrome P450 108B4 Cyp108B4 complement(4318186..4319418) Mycobacterium ulcerans Agy99 4553898 YP_907416.1 CDS accD4_2 NC_008611.1 4319609 4321105 D cytoplasmic protein; key enzyme in the catabolic pathway of odd-chain fatty acids, isoleucine, threonine, methionine, and valine [catalytic activity: ATP + propionyl-CoA + CO(2) + H(2)O = ADP + orthophosphate + methylmalonyl-CoA]; propionyl-CoA carboxylase beta chain 4 AccD4_2 4319609..4321105 Mycobacterium ulcerans Agy99 4553899 YP_907417.1 CDS MUL_3865 NC_008611.1 4321095 4322645 R May not be functional. C-term of the dehydrogenase section missing 40 aa. may be site of DNA deletion, resulting in a translation fusion with a d/s ORF . C-term contains full length of an uncharacterized homolog of a gamma-carboxymuconolactone decarboxylase subunit; membrane protein; dehydrogenase/decarboxylase protein complement(4321095..4322645) Mycobacterium ulcerans Agy99 4553900 YP_907418.1 CDS MUL_3866 NC_008611.1 4322829 4323875 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase complement(4322829..4323875) Mycobacterium ulcerans Agy99 4553901 YP_907419.1 CDS MUL_3867 NC_008611.1 4323872 4324255 R cytoplasmic protein; function unknown but contains a Ntf2-like superfmaily domain; hypothetical protein complement(4323872..4324255) Mycobacterium ulcerans Agy99 4553902 YP_907420.1 CDS MUL_3868 NC_008611.1 4324252 4324488 R secreted protein; hypothetical protein complement(4324252..4324488) Mycobacterium ulcerans Agy99 4553903 YP_907421.1 CDS MUL_3869 NC_008611.1 4324619 4325236 D cytoplasmic protein; function unknown but contains thioesterase/thiol ester dehydrase-isomerase superfmaily domain; hypothetical protein 4324619..4325236 Mycobacterium ulcerans Agy99 4553904 YP_907422.1 CDS fadD19_2 NC_008611.1 4325324 4326967 D activates fatty acids by binding to coenzyme A; in Mycobacterium may be involved in virulence; acyl-CoA synthetase 4325324..4326967 Mycobacterium ulcerans Agy99 4553905 YP_907423.1 CDS MUL_3871 NC_008611.1 4327067 4328113 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(4327067..4328113) Mycobacterium ulcerans Agy99 4553906 YP_907424.1 CDS cyp187A5 NC_008611.1 4328422 4329636 D cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.; cytochrome P450 187A5 Cyp187A5 4328422..4329636 Mycobacterium ulcerans Agy99 4553907 YP_907425.1 CDS MUL_3873 NC_008611.1 4329769 4330860 D cytoplasmic protein; dehydratase 4329769..4330860 Mycobacterium ulcerans Agy99 4553908 YP_907426.1 CDS MUL_3874 NC_008611.1 4330882 4332063 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase complement(4330882..4332063) Mycobacterium ulcerans Agy99 4553909 YP_907427.1 CDS MUL_3876 NC_008611.1 4333139 4333975 R cytoplasmic protein; metal-dependent hydrolase complement(4333139..4333975) Mycobacterium ulcerans Agy99 4553911 YP_907428.1 CDS MUL_3878 NC_008611.1 4335626 4336831 R cytoplasmic protein; metal-dependent hydrolase complement(4335626..4336831) Mycobacterium ulcerans Agy99 4553785 YP_907429.1 CDS echA12_1 NC_008611.1 4336860 4337702 R Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase complement(4336860..4337702) Mycobacterium ulcerans Agy99 4553786 YP_907430.1 CDS MUL_3882 NC_008611.1 4338801 4339445 R cytoplasmic protein; hypothetical protein complement(4338801..4339445) Mycobacterium ulcerans Agy99 4553788 YP_907431.1 CDS MUL_3883 NC_008611.1 4339666 4339995 D cytoplasmic protein; hypothetical protein 4339666..4339995 Mycobacterium ulcerans Agy99 4553789 YP_907432.1 CDS MUL_3884 NC_008611.1 4340399 4341202 D cytoplasmic protein; involved in transcriptional mechanism; transcriptional regulatory protein 4340399..4341202 Mycobacterium ulcerans Agy99 4553790 YP_907433.1 CDS echA4_2 NC_008611.1 4341215 4342111 R cytoplasmic protein; oxidizes fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]; enoyl-CoA hydratase, EchA4_2 complement(4341215..4342111) Mycobacterium ulcerans Agy99 4553791 YP_907434.1 CDS MUL_3886 NC_008611.1 4342108 4342782 R cytoplasmic protein; hypothetical protein complement(4342108..4342782) Mycobacterium ulcerans Agy99 4553792 YP_907435.1 CDS echA10_1 NC_008611.1 4342902 4343720 D cytoplasmic protein; oxidizes fatty acids using specific components (by similarity) [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]; enoyl-CoA hydratase EchA10 4342902..4343720 Mycobacterium ulcerans Agy99 4553793 YP_907436.1 CDS echA11_1 NC_008611.1 4343717 4344535 D cytoplasmic protein; oxidizes fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]; enoyl-CoA hydratase EchA11 4343717..4344535 Mycobacterium ulcerans Agy99 4553794 YP_907437.1 CDS echA16_1 NC_008611.1 4344526 4345341 D cytoplasmic protein; oxidizes fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]; enoyl-CoA hydratase EchA16 4344526..4345341 Mycobacterium ulcerans Agy99 4553795 YP_907438.1 CDS MUL_3890 NC_008611.1 4345341 4346822 D cytoplasmic protein; function unknown, but supposed involvement in lipid degradation.; long-chain fatty-acid CoA ligase 4345341..4346822 Mycobacterium ulcerans Agy99 4553796 YP_907439.1 CDS MUL_3893 NC_008611.1 4350245 4350610 R secreted protein; hypothetical protein complement(4350245..4350610) Mycobacterium ulcerans Agy99 4553799 YP_907440.1 CDS MUL_3894 NC_008611.1 4350811 4351524 R cytoplasmic protein; possibly involved in transcriptional mechanism.; GntR family transcriptional regulator complement(4350811..4351524) Mycobacterium ulcerans Agy99 4553800 YP_907441.1 CDS MUL_3895 NC_008611.1 4351538 4352731 R cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase complement(4351538..4352731) Mycobacterium ulcerans Agy99 4553801 YP_907442.1 CDS MUL_3896 NC_008611.1 4352728 4353957 R function unknown, but supposed involvement in lipid degradation.; acyl-CoA transferase/carnitine dehydratase complement(4352728..4353957) Mycobacterium ulcerans Agy99 4553802 YP_907443.1 CDS fabG NC_008611.1 4355179 4355931 R Catalyzes the first of the two reduction steps in the elongation cycle of fatty acid synthesis; 3-ketoacyl-ACP reductase complement(4355179..4355931) Mycobacterium ulcerans Agy99 4553714 YP_907444.1 CDS MUL_3901 NC_008611.1 4357441 4359180 R membrane protein; thought to be involved in active transport of drugs across the membrane (export): multidrugs resistance by an export mechanism. responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane.; transmembrane ABC transporter ATP-binding protein complement(4357441..4359180) Mycobacterium ulcerans Agy99 4553717 YP_907445.1 CDS MUL_3902 NC_008611.1 4359177 4361801 R membrane protein; thought to be involved in active transport of drugs across the membrane (export): multidrugs resistance by an export mechanism. responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane.; transmembrane ABC transporter ATP-binding protein complement(4359177..4361801) Mycobacterium ulcerans Agy99 4553718 YP_907446.1 CDS lprD NC_008611.1 4362538 4362930 D membrane protein; lipoprotein LprD 4362538..4362930 Mycobacterium ulcerans Agy99 4553720 YP_907447.1 CDS MUL_3905 NC_008611.1 4362927 4363289 D membrane protein; hypothetical protein 4362927..4363289 Mycobacterium ulcerans Agy99 4553721 YP_907448.1 CDS MUL_3906 NC_008611.1 4363309 4363923 R HAM1-like protein; Rec-dependent growth; RgdB; yggV; it is suspected that this protein functions to remove misincorporated bases such as xanthine or hypoxanthine; putative deoxyribonucleotide triphosphate pyrophosphatase complement(4363309..4363923) Mycobacterium ulcerans Agy99 4553722 YP_907449.1 CDS MUL_3907 NC_008611.1 4364076 4364618 R membrane protein; hypothetical protein complement(4364076..4364618) Mycobacterium ulcerans Agy99 4553723 YP_907450.1 CDS rph NC_008611.1 4364899 4365678 R RNase PH; tRNA nucleotidyltransferase; forms hexamers in Bacillus subtilis; phosphoroltic 3'-5' exoribonuclease; involved in maturation of tRNA precursors and removes terminal nucleotides near CCA acceptor arms of mature tRNAs; ribonuclease PH complement(4364899..4365678) Mycobacterium ulcerans Agy99 4553724 YP_907451.1 CDS MUL_3909 NC_008611.1 4365780 4366553 R cytoplasmic protein; metal-dependent hydrolase complement(4365780..4366553) Mycobacterium ulcerans Agy99 4553725 YP_907452.1 CDS MUL_3910 NC_008611.1 4366553 4366732 R cytoplasmic protein; hypothetical protein complement(4366553..4366732) Mycobacterium ulcerans Agy99 4553726 YP_907453.1 CDS MUL_3911 NC_008611.1 4366931 4367662 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 4366931..4367662 Mycobacterium ulcerans Agy99 4553727 YP_907454.1 CDS aceE_1 NC_008611.1 4367929 4370256 R cytoplasmic protein; involved in energy metabolism; contributes to acetyl-CoA production as part of pyruvate dehydrogenase complex [catalytic activity: pyruvate + lipoamide = S- acetyl-dihydro-lipoamide + CO(2)]; pyruvate dehydrogenase E1 component AceE complement(4367929..4370256) Mycobacterium ulcerans Agy99 4553728 YP_907455.1 CDS MUL_3913 NC_008611.1 4370365 4370883 D cytoplasmic protein; involved in transcriptional mechanism.; Lrp/AsnC family transcriptional regulator 4370365..4370883 Mycobacterium ulcerans Agy99 4553729 YP_907456.1 CDS MUL_3914 NC_008611.1 4371250 4371750 D secreted protein; hypothetical protein 4371250..4371750 Mycobacterium ulcerans Agy99 4553730 YP_907457.1 CDS yrbE3A_1 NC_008611.1 4372513 4373280 D membrane protein; integral membrane protein YrbE3A 4372513..4373280 Mycobacterium ulcerans Agy99 4553585 YP_907458.1 CDS yrbE3B_1 NC_008611.1 4373277 4374131 D membrane protein; integral membrane protein YrbE3B 4373277..4374131 Mycobacterium ulcerans Agy99 4553586 YP_907459.1 CDS mce3A_1 NC_008611.1 4374136 4375596 D membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE-family protein Mce3A 4374136..4375596 Mycobacterium ulcerans Agy99 4553587 YP_907460.1 CDS mce3B_1 NC_008611.1 4375676 4376707 D membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE-family protein Mce3B 4375676..4376707 Mycobacterium ulcerans Agy99 4553588 YP_907461.1 CDS mce3C_1 NC_008611.1 4376704 4378020 D membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE-family protein, Mce3C 4376704..4378020 Mycobacterium ulcerans Agy99 4553589 YP_907462.1 CDS mce3D_1 NC_008611.1 4378017 4379348 D membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE-family protein Mce3D 4378017..4379348 Mycobacterium ulcerans Agy99 4553590 YP_907463.1 CDS lprM_1 NC_008611.1 4379348 4380490 D membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE family lipoprotein LprM 4379348..4380490 Mycobacterium ulcerans Agy99 4553591 YP_907464.1 CDS mce3F_1 NC_008611.1 4380492 4381949 D membrane protein; function unknown, but thought involved in host cell invasion.; MCE-family protein Mce3F 4380492..4381949 Mycobacterium ulcerans Agy99 4553592 YP_907465.1 CDS MUL_3924 NC_008611.1 4381946 4382647 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; Mce associated membrane protein 4381946..4382647 Mycobacterium ulcerans Agy99 4553593 YP_907466.1 CDS murI NC_008611.1 4382690 4383505 R converts L-glutamate to D-glutamate, a component of peptidoglycan; glutamate racemase complement(4382690..4383505) Mycobacterium ulcerans Agy99 4553594 YP_907467.1 CDS cfp10A NC_008611.1 4384351 4384623 R secreted protein; antigen Cfp10A complement(4384351..4384623) Mycobacterium ulcerans Agy99 4553596 YP_907468.1 CDS MUL_3928 NC_008611.1 4384645 4385058 R cytoplasmic protein; function unknown but domain identity suggests this CDS encodes a metal-dependent protease of the Pad1/Jab1 superfamily.; hypothetical protein complement(4384645..4385058) Mycobacterium ulcerans Agy99 4553597 YP_907469.1 CDS dmpA NC_008611.1 4385060 4386148 R membrane protein; function unknown, probable role in amino acid hydrolysis; L-aminopeptidase/D-esterase DmpA complement(4385060..4386148) Mycobacterium ulcerans Agy99 4553598 YP_907470.1 CDS MUL_3930 NC_008611.1 4386145 4386732 R cytoplasmic protein; hypothetical protein complement(4386145..4386732) Mycobacterium ulcerans Agy99 4553599 YP_907471.1 CDS MUL_3931 NC_008611.1 4386757 4386969 R cytoplasmic protein; function unknown but contains a ClpS domain suggesting a function related to ATP-dependent protein degradation.; hypothetical protein complement(4386757..4386969) Mycobacterium ulcerans Agy99 4553600 YP_907472.1 CDS pncB NC_008611.1 4387128 4388444 D catalyzes the formation of 5-phospho-alpha-D-ribose 1-diphosphate and nicotinate from nicotinate D-ribonucleotide and diphosphate; nicotinate phosphoribosyltransferase 4387128..4388444 Mycobacterium ulcerans Agy99 4553601 YP_907473.1 CDS dinG NC_008611.1 4388480 4390501 D cytoplasmic protein; probable helicase involved in DNA repair and perhaps also replication.; ATP-dependent helicase DinG 4388480..4390501 Mycobacterium ulcerans Agy99 4553602 YP_907474.1 CDS glgP NC_008611.1 4390498 4393089 R cytoplasmic protein; catalytic activity : {(1,4)-alpha-D-glucosyl}(N) + phosphate = {(1,4)-alpha-D-glucosyl}(N-1) + alpha-D- glucose 1-phosphate.; glycogen phosphorylase GlgP complement(4390498..4393089) Mycobacterium ulcerans Agy99 4553603 YP_907475.1 CDS MUL_3935 NC_008611.1 4393279 4395366 D also detected in the membrane fraction by proteomics (LC-MS/MS); cytoplasmic protein; function unknown, may be involved in polysaccharide degradation.; glycosidase 4393279..4395366 Mycobacterium ulcerans Agy99 4553604 YP_907476.1 CDS glgB NC_008611.1 4395373 4397568 D catalyzes the transfer of a segment of a 1,4-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain; glycogen branching protein 4395373..4397568 Mycobacterium ulcerans Agy99 4552503 YP_907477.1 CDS MUL_3937 NC_008611.1 4397635 4398549 R Also detected in the extracellular matrix and secreted fraction by proteomics.; extracellular matrix protein; thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions; thioredoxin complement(4397635..4398549) Mycobacterium ulcerans Agy99 4552504 YP_907478.1 CDS fadA4 NC_008611.1 4398727 4399908 R Catalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation; in Rhizobia and Ralstonia is involved in PHB biosynthesis; acetyl-CoA acetyltransferase complement(4398727..4399908) Mycobacterium ulcerans Agy99 4552505 YP_907479.1 CDS gloA_1 NC_008611.1 4399991 4400458 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; glyoxalase I (lactoylglutathione lyase) catalyzes the first step of the glyoxal pathway.; glyoxalase GloA 4399991..4400458 Mycobacterium ulcerans Agy99 4552506 YP_907480.1 CDS MUL_3940 NC_008611.1 4400771 4401451 R cytoplasmic protein; function unknown but contains a predicted nuclease domain of the RecB family; hypothetical protein complement(4400771..4401451) Mycobacterium ulcerans Agy99 4552507 YP_907481.1 CDS MUL_3941 NC_008611.1 4401542 4402876 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(4401542..4402876) Mycobacterium ulcerans Agy99 4552508 YP_907482.1 CDS MUL_3943 NC_008611.1 4404115 4405161 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 4404115..4405161 Mycobacterium ulcerans Agy99 4552510 YP_907483.1 CDS MUL_3944 NC_008611.1 4406071 4407690 D membrane protein; thought to play a role in regulation of cellular metabolism by catalysing the synthesis of a second messenger, camp [catalytic activity: ATP = 3',5'-cyclic AMP + pyrophosphate]; adenylate cyclase 4406071..4407690 Mycobacterium ulcerans Agy99 4552511 YP_907484.1 CDS alkA NC_008611.1 4407773 4409260 D cytoplasmic protein; involved in damage reversal and in base excision repair. the methylated Ada protein acts as a positive regulator of its own synthesis, as well as that of other proteins. the transcription-activating function of the Ada protein resides in its N-terminus. repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. this is a suicide reaction: the enzyme is irreversibly inactivated. can also repair O-4- methylthymine [catalytic activity: DNA (containing 6-O- methylguanine) + [protein]-L-cysteine = DNA (without 6-O- methylguanine) + protein S-methyl-L-cysteine]; Ada regulatory protein AlkA 4407773..4409260 Mycobacterium ulcerans Agy99 4552512 YP_907485.1 CDS ogt NC_008611.1 4409257 4409775 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); cytoplasmic protein; repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. this is a suicide reaction: the enzyme is irreversibly inactivated [catalytic activity : DNA (containing 6-O-methylguanine) + [protein]-L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine]; methylated-DNA--protein-cysteine methyltransferase Ogt 4409257..4409775 Mycobacterium ulcerans Agy99 4552513 YP_907486.1 CDS murA NC_008611.1 4415229 4416482 R adds enolpyruvyl to UDP-N-acetylglucosamine as a component of cell wall formation; gram-positive bacteria have 2 copies of MurA which are active; UDP-N-acetylglucosamine 1-carboxyvinyltransferase complement(4415229..4416482) Mycobacterium ulcerans Agy99 4552517 YP_907487.1 CDS MUL_3951 NC_008611.1 4416553 4417146 D Detected in the cytoplamic fraction by LC-MS/MS. Also detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; these proteins convert inactive cobalamins to AdoCbl for 1,2-propanediol degradation.; cobalamin adenosyltransferase 4416553..4417146 Mycobacterium ulcerans Agy99 4552587 YP_907488.1 CDS MUL_3952 NC_008611.1 4417237 4417680 R cytoplasmic protein; hypothetical protein complement(4417237..4417680) Mycobacterium ulcerans Agy99 4552588 YP_907489.1 CDS atpC NC_008611.1 4417688 4418068 R part of catalytic core of ATP synthase; alpha(3)beta(3)gamma(1)delta(1)epsilon(1); involved in producing ATP from ADP in the presence of the proton motive force across the membrane; F0F1 ATP synthase subunit epsilon complement(4417688..4418068) Mycobacterium ulcerans Agy99 4552589 YP_907490.1 CDS atpD NC_008611.1 4418105 4419556 R Produces ATP from ADP in the presence of a proton gradient across the membrane. The beta chain is a regulatory subunit; F0F1 ATP synthase subunit beta complement(4418105..4419556) Mycobacterium ulcerans Agy99 4552590 YP_907491.1 CDS atpG NC_008611.1 4419585 4420499 R Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is a regulatory subunit; F0F1 ATP synthase subunit gamma complement(4419585..4420499) Mycobacterium ulcerans Agy99 4552591 YP_907492.1 CDS atpA NC_008611.1 4420518 4422167 R produces ATP from ADP in the presence of a proton gradient across the membrane; the alpha chain is a catalytic subunit; F0F1 ATP synthase subunit alpha complement(4420518..4422167) Mycobacterium ulcerans Agy99 4552592 YP_907493.1 CDS atpH NC_008611.1 4422229 4423566 R produces ATP from ADP in the presence of a proton gradient across the membrane; the delta subunit is part of the catalytic core of the ATP synthase complex; F0F1 ATP synthase subunit delta complement(4422229..4423566) Mycobacterium ulcerans Agy99 4552593 YP_907494.1 CDS atpF NC_008611.1 4423573 4424085 R Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit B is part of the membrane proton channel.; F0F1 ATP synthase subunit B complement(4423573..4424085) Mycobacterium ulcerans Agy99 4552594 YP_907495.1 CDS atpE NC_008611.1 4424127 4424372 R Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit C is part of the membrane proton channel F0; F0F1 ATP synthase subunit C complement(4424127..4424372) Mycobacterium ulcerans Agy99 4552595 YP_907496.1 CDS atpB NC_008611.1 4424463 4425215 R Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit A is part of the membrane proton channel F0; F0F1 ATP synthase subunit A complement(4424463..4425215) Mycobacterium ulcerans Agy99 4552596 YP_907497.1 CDS MUL_3961 NC_008611.1 4425184 4425708 R membrane protein; hypothetical protein complement(4425184..4425708) Mycobacterium ulcerans Agy99 4552597 YP_907498.1 CDS rfe NC_008611.1 4425958 4427139 R membrane protein; catalysis of the first step of the lipid cycle reactions in the biosynthesis of cell wall peptidoglycan.; undecapaprenyl-phosphate alpha-N- acetylglucosaminyltransferase Rfe complement(4425958..4427139) Mycobacterium ulcerans Agy99 4552598 YP_907499.1 CDS MUL_3963 NC_008611.1 4427218 4427868 R function unknown but contains YrdC domain. this domain has been shown to preferentially bind to dsRNA.; hypothetical protein complement(4427218..4427868) Mycobacterium ulcerans Agy99 4552599 YP_907500.1 CDS hemK NC_008611.1 4427921 4428769 R cytoplasmic protein; function unknown but may have S-adenosyl-methionine- dependent methyltransferase activity.; modification methylase HemK complement(4427921..4428769) Mycobacterium ulcerans Agy99 4552600 YP_907501.1 CDS prfA NC_008611.1 4428766 4429839 R recognizes the termination signals UAG and UAA during protein translation a specificity which is dependent on amino acid residues residing in loops of the L-shaped tRNA-like molecule of RF1; this protein is similar to release factor 2; peptide chain release factor 1 complement(4428766..4429839) Mycobacterium ulcerans Agy99 4552601 YP_907502.1 CDS rpmE NC_008611.1 4429927 4430172 R RpmE; there appears to be two types of ribosomal proteins L31 in bacterial genomes; some contain a CxxC motif while others do not; Bacillus subtilis has both types; the proteins in this cluster have the CXXC motif; RpmE is found in exponentially growing Bacilli while YtiA was found after exponential growth; expression of ytiA is controlled by a zinc-specific transcriptional repressor; RpmE contains one zinc ion and a CxxC motif is responsible for this binding; forms an RNP particle along with proteins L5, L18, and L25 and 5S rRNA; found crosslinked to L2 and L25 and EF-G; may be near the peptidyltransferase site of the 50S ribosome; 50S ribosomal protein L31 complement(4429927..4430172) Mycobacterium ulcerans Agy99 4552602 YP_907503.1 CDS rho NC_008611.1 4430364 4432229 R An RNA-DNA helicase that actively releases nascent mRNAs from paused transcription complexes; transcription termination factor Rho complement(4430364..4432229) Mycobacterium ulcerans Agy99 4552603 YP_907504.1 CDS thrB NC_008611.1 4432496 4433440 R catalyzes the formation of O-phospho-L-homoserine from L-homoserine in threonine biosynthesis from asparate; homoserine kinase complement(4432496..4433440) Mycobacterium ulcerans Agy99 4552604 YP_907505.1 CDS thrC NC_008611.1 4433496 4434578 R catalyzes the formation of L-threonine from O-phospho-L-homoserine; threonine synthase complement(4433496..4434578) Mycobacterium ulcerans Agy99 4552605 YP_907506.1 CDS thrA NC_008611.1 4434575 4435900 R catalyzes the formation of L-aspartate 4-semialdehyde from L-homoserine; homoserine dehydrogenase complement(4434575..4435900) Mycobacterium ulcerans Agy99 4551394 YP_907507.1 CDS lysA NC_008611.1 4435904 4437322 R cytoplasmic protein; involved in biosynthesis of lysine (last step) [catalytic activity : meso-2,6-diaminoheptanedioate = L- lysine + CO(2)]; diaminopimelate decarboxylase LysA complement(4435904..4437322) Mycobacterium ulcerans Agy99 4551395 YP_907508.1 CDS argS NC_008611.1 4437319 4438971 R catalyzes a two-step reaction, first charging an arginine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA synthetase; arginyl-tRNA synthetase complement(4437319..4438971) Mycobacterium ulcerans Agy99 4551396 YP_907509.1 CDS MUL_3974 NC_008611.1 4439665 4439940 D cytoplasmic protein; hypothetical protein 4439665..4439940 Mycobacterium ulcerans Agy99 4551398 YP_907510.1 CDS MUL_3975 NC_008611.1 4440081 4440611 R membrane protein; PE-PGRS family protein complement(4440081..4440611) Mycobacterium ulcerans Agy99 4551399 YP_907511.1 CDS MUL_3976 NC_008611.1 4441115 4443784 D cytoplasmic protein; this family of dehydrogenases act on aldehyde substrates. members use NADP as a cofactor.; bifunctional acetaldehyde-CoA/alcohol dehydrogenase 4441115..4443784 Mycobacterium ulcerans Agy99 4551400 YP_907512.1 CDS MUL_3977 NC_008611.1 4443750 4444127 R cytoplasmic protein; pyridoxamine 5'-phosphate oxidase is a FMN flavoprotein involved in the de novo synthesis of pyridoxine (vitamin B6) and pyridoxal phosphate.; pyridoxamine 5'-phosphate oxidase-related protein complement(4443750..4444127) Mycobacterium ulcerans Agy99 4551401 YP_907513.1 CDS MUL_3978 NC_008611.1 4444223 4444594 R cytoplasmic protein; function unknown but contains YCII-related protein domain; hypothetical protein complement(4444223..4444594) Mycobacterium ulcerans Agy99 4551402 YP_907514.1 CDS dhaA NC_008611.1 4444575 4445447 R catalyzes the cleavage of carbon-halogen bonds in aliphatic compounds forming a primary alcohol and a halide; haloalkane dehalogenase complement(4444575..4445447) Mycobacterium ulcerans Agy99 4551403 YP_907515.1 CDS MUL_3980 NC_008611.1 4445498 4447288 R cytoplasmic protein; cleaves xaa-pro-releasing N-terminal dipeptides.; peptidase S15 complement(4445498..4447288) Mycobacterium ulcerans Agy99 4551404 YP_907516.1 CDS MUL_3981 NC_008611.1 4447429 4448076 D cytoplasmic protein; function unknown but belongs to the TetR family of bacterial transcriptional regulatory proteins.; putative regulatory protein 4447429..4448076 Mycobacterium ulcerans Agy99 4551405 YP_907517.1 CDS MUL_3982 NC_008611.1 4448086 4448598 R membrane protein; PE-PGRS family protein complement(4448086..4448598) Mycobacterium ulcerans Agy99 4551406 YP_907518.1 CDS MUL_3983 NC_008611.1 4448729 4449634 R cytoplasmic protein; function unknown, belongs to the LysR family of transcriptional regulators; putative regulatory protein complement(4448729..4449634) Mycobacterium ulcerans Agy99 4551407 YP_907519.1 CDS MUL_3984 NC_008611.1 4449725 4451053 D membrane protein; function unknown role in transport. belongs to the DMT superfamily.; membrane permease 4449725..4451053 Mycobacterium ulcerans Agy99 4551408 YP_907520.1 CDS MUL_3985 NC_008611.1 4451034 4451585 D membrane protein; hypothetical protein 4451034..4451585 Mycobacterium ulcerans Agy99 4551409 YP_907521.1 CDS MUL_3986 NC_008611.1 4451780 4453366 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; thought to play a role in regulation of cellular metabolism by catalysing the synthesis of a second messenger, camp [catalytic activity: ATP = 3',5'-cyclic AMP + pyrophosphate]; adenylate cyclase 4451780..4453366 Mycobacterium ulcerans Agy99 4551410 YP_907522.1 CDS MUL_3987 NC_008611.1 4453532 4453873 D secreted protein; hypothetical protein 4453532..4453873 Mycobacterium ulcerans Agy99 4551411 YP_907523.1 CDS MUL_3990 NC_008611.1 4456652 4457851 R membrane protein; PE-PGRS family protein complement(4456652..4457851) Mycobacterium ulcerans Agy99 4550730 YP_907524.1 CDS MUL_3991 NC_008611.1 4457949 4458821 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; flavin-dependent oxidoreductase complement(4457949..4458821) Mycobacterium ulcerans Agy99 4550731 YP_907525.1 CDS gloA_2 NC_008611.1 4458867 4459289 D cytoplasmic protein; catalyzes the first step of the glyoxal pathway; glyoxalase GloA 4458867..4459289 Mycobacterium ulcerans Agy99 4550732 YP_907526.1 CDS MUL_3993 NC_008611.1 4459382 4459771 D cytoplasmic protein; function unknown but contains glyoxalase/bleomycin resistance protein/Dihydroxybiphenyl dioxygenase superfamily domain.; hypothetical protein 4459382..4459771 Mycobacterium ulcerans Agy99 4550733 YP_907527.1 CDS MUL_3994 NC_008611.1 4459768 4460253 R cytoplasmic protein; putative transcriptional regulatory protein complement(4459768..4460253) Mycobacterium ulcerans Agy99 4550734 YP_907528.1 CDS cysN NC_008611.1 4460347 4462197 R in Rhizobium meliloti this protein is involved in the synthesis of nodulation factors that are active on the roots of alfalfa; catalyzes formation of activated sulfate intermediate; converts ATP and sulfate to diphosphate and adenylylsulfate and then ATP and adenylyl sulfate to ADP and 3'-phosphoadenylyl sulfate; the activated intermediate is transferred to the nodulation factors by NodH; may interact with NodP and NodQ; similar to the CysD and CysN proteins from EScherichia coli involved in cysteine biosynthesis; bifunctional sulfate adenylyltransferase subunit 1/adenylylsulfate kinase complement(4460347..4462197) Mycobacterium ulcerans Agy99 4550735 YP_907529.1 CDS cysD NC_008611.1 4462197 4463129 R with CysN catalyzes the formation of adenylylsulfate from sulfate and ATP; sulfate adenylyltransferase subunit 2 complement(4462197..4463129) Mycobacterium ulcerans Agy99 4550736 YP_907530.1 CDS MUL_3997 NC_008611.1 4463317 4463751 D cytoplasmic protein; function unknown, contains a thioesterase superfamily domain; hypothetical protein 4463317..4463751 Mycobacterium ulcerans Agy99 4550737 YP_907531.1 CDS MUL_3998 NC_008611.1 4463759 4464250 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the extracellular matrix and membrane fraction by proteomics.; membrane protein; domain identity to carbonic anhydrases. these are zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide.; carbonic anhydrase complement(4463759..4464250) Mycobacterium ulcerans Agy99 4550738 YP_907532.1 CDS oppB NC_008611.1 4464573 4465550 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in active transport of oligopeptide across the membrane (import) responsible for the translocation of the substrate across the membrane.; oligopeptide-transport integral membrane protein ABC transporter OppB 4464573..4465550 Mycobacterium ulcerans Agy99 4550739 YP_907533.1 CDS oppC NC_008611.1 4465547 4466440 D membrane protein; involved in active transport of oligopeptide across the membrane (import) responsible for the translocation of the substrate across the membrane.; oligopeptide-transport integral membrane protein ABC transporter OppC 4465547..4466440 Mycobacterium ulcerans Agy99 4550740 YP_907534.1 CDS oppD NC_008611.1 4466437 4468275 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in active transport of oligopeptide across the membrane (import) responsible for energy coupling to the transport system.; oligopeptide-transport ATP-binding protein ABC transporter OppD 4466437..4468275 Mycobacterium ulcerans Agy99 4550741 YP_907535.1 CDS oppA NC_008611.1 4468376 4470013 D Also detected in the cytoplasm.; membrane protein; involved in active transport of oligopeptide across the membrane (import) this protein is a component of the oligopeptide permease, a binding protein-dependent transport system; it binds peptides up to five amino acids long with high affinity.; periplasmic oligopeptide-binding lipoprotein OppA 4468376..4470013 Mycobacterium ulcerans Agy99 4550742 YP_907536.1 CDS MUL_4004 NC_008611.1 4471338 4472927 R cytoplasmic protein; function unknown, probably involved in cellular metabolism, probably electron-transfer-linked.; dehydrogenase fad flavoprotein GMC oxidoreductase complement(4471338..4472927) Mycobacterium ulcerans Agy99 4550744 YP_907537.1 CDS MUL_4005 NC_008611.1 4472977 4475607 R Detected in the cytoplamic fraction by LCMSMS; cytoplasmic protein; function unknown, contains P-loop containing nucleoside triphosphate hydrolase superfamily domain; hydrolase complement(4472977..4475607) Mycobacterium ulcerans Agy99 4550745 YP_907538.1 CDS sbcD NC_008611.1 4475604 4476755 R cytoplasmic protein; function unknown: possible role in DNA replication, recombination, and repair.; DNA repair exonuclease SbcD complement(4475604..4476755) Mycobacterium ulcerans Agy99 4550746 YP_907539.1 CDS sixA NC_008611.1 4477041 4477544 D cytoplasmic protein; function unknown, possible role in signal transduction; phosphohistidine phosphatase SixA 4477041..4477544 Mycobacterium ulcerans Agy99 4549954 YP_907540.1 CDS lprC NC_008611.1 4477604 4478182 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; lipoprotein LprC complement(4477604..4478182) Mycobacterium ulcerans Agy99 4549955 YP_907541.1 CDS lprB NC_008611.1 4478179 4478736 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; lipoprotein LprB complement(4478179..4478736) Mycobacterium ulcerans Agy99 4549956 YP_907542.1 CDS MUL_4010 NC_008611.1 4478884 4480623 D membrane protein; thought to be involved in active transport of drugs across the membrane (export): multidrugs resistance by an export mechanism. responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane.; transmembrane ABC transporter ATP-binding protein 4478884..4480623 Mycobacterium ulcerans Agy99 4549957 YP_907543.1 CDS MUL_4011 NC_008611.1 4480620 4482542 D membrane protein; thought to be involved in active transport of drugs across the membrane (export): multidrugs resistance by an export mechanism. responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane.; transmembrane ABC transporter ATP-binding protein 4480620..4482542 Mycobacterium ulcerans Agy99 4549958 YP_907544.1 CDS MUL_4013 NC_008611.1 4485121 4485453 D secreted protein; hypothetical protein 4485121..4485453 Mycobacterium ulcerans Agy99 4549960 YP_907545.1 CDS MUL_4014 NC_008611.1 4485685 4486017 D secreted protein; hypothetical protein 4485685..4486017 Mycobacterium ulcerans Agy99 4549961 YP_907546.1 CDS lprA NC_008611.1 4486257 4486997 D membrane protein; lipoprotein LprA 4486257..4486997 Mycobacterium ulcerans Agy99 4549962 YP_907547.1 CDS MUL_4016 NC_008611.1 4487114 4487449 D secreted protein; hypothetical protein 4487114..4487449 Mycobacterium ulcerans Agy99 4549963 YP_907548.1 CDS MUL_4017 NC_008611.1 4487593 4488303 D secreted protein; hypothetical protein 4487593..4488303 Mycobacterium ulcerans Agy99 4549964 YP_907549.1 CDS embR NC_008611.1 4488640 4489800 D cytoplasmic protein; involved in transcriptional mechanism. thought to regulate the biosynthesis of the mycobacterial cell wall arabinan and resistance to ethambutol (Emb; dextro-2,2'- (ethylenediimino)-di-1-butanol), regulating EmbA and EmbB.; transcriptional regulatory protein EmbR 4488640..4489800 Mycobacterium ulcerans Agy99 4549965 YP_907550.1 CDS MUL_4020 NC_008611.1 4492344 4493246 R cytoplasmic protein; LuxR family transcriptional regulator complement(4492344..4493246) Mycobacterium ulcerans Agy99 4549967 YP_907551.1 CDS citE_1 NC_008611.1 4493352 4494200 D Detected in the cytoplasmic fraction by LC-MS/MS. Also detected in the extracellular matrix and membrane fraction by proteomics.; cytoplasmic protein; interconversion of acetate and oxaloacetate from citrate [catalytic activity: citrate = acetate + oxaloacetate]; citrate (pro-3S)-lyase subunit beta 4493352..4494200 Mycobacterium ulcerans Agy99 4549968 YP_907552.1 CDS MUL_4022 NC_008611.1 4494188 4494715 D Detected in the cytoplasmic fraction by LC-MS/MS. Also detected in the membrane fraction by proteomics (2D- LC-MS/MS); cytoplasmic protein; dehydratase 4494188..4494715 Mycobacterium ulcerans Agy99 4549969 YP_907553.1 CDS MUL_4023 NC_008611.1 4494715 4496052 D Detected in the membrane fraction by proteomics (LC- MS/MS) Also detected in the cytoplamic fraction by LCMSMS; membrane protein; could be implicated in several pathways involving acetyl-CoA. E.G. acetyl-CoA hydrolase from yeast catalyses the formation of acetate from acetyl-CoA, CoA transferase (CAT1) produces succinyl-CoA, and acetate-CoA transferase utilizes acyl-CoA and acetate to form acetyl-CoA.; acetyl-CoA hydrolase/transferase 4494715..4496052 Mycobacterium ulcerans Agy99 4549970 YP_907554.1 CDS fadE3 NC_008611.1 4496070 4497245 D Detected in the cytoplasmic and membrane fractionc by proteomics.; cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE3 4496070..4497245 Mycobacterium ulcerans Agy99 4549971 YP_907555.1 CDS gdpD NC_008611.1 4497277 4498185 R membrane protein; hydrolyses glycerophosphodiesters such as glycerophosphocholine, glycerophosphoethanolamine, glycerophosphoglycerol, and bis(glycerophosphoglycerol); glycerolphosphodiesterase GdpD complement(4497277..4498185) Mycobacterium ulcerans Agy99 4549972 YP_907556.1 CDS MUL_4026 NC_008611.1 4498378 4498803 R cytoplasmic protein; hypothetical protein complement(4498378..4498803) Mycobacterium ulcerans Agy99 4550081 YP_907557.1 CDS MUL_4027 NC_008611.1 4498875 4499507 D secreted protein; hypothetical protein 4498875..4499507 Mycobacterium ulcerans Agy99 4550082 YP_907558.1 CDS gloA_3 NC_008611.1 4499576 4499995 R cytoplasmic protein; glyoxalase I (lactoylglutathione lyase) catalyzes the first step of the glyoxal pathway.; glyoxalase GloA complement(4499576..4499995) Mycobacterium ulcerans Agy99 4550083 YP_907559.1 CDS MUL_4029 NC_008611.1 4500714 4502048 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(4500714..4502048) Mycobacterium ulcerans Agy99 4550084 YP_907560.1 CDS MUL_4030 NC_008611.1 4502436 4503140 D cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 4502436..4503140 Mycobacterium ulcerans Agy99 4550085 YP_907561.1 CDS MUL_4031 NC_008611.1 4503165 4503482 D cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 4503165..4503482 Mycobacterium ulcerans Agy99 4550086 YP_907562.1 CDS MUL_4034 NC_008611.1 4504820 4505509 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(4504820..4505509) Mycobacterium ulcerans Agy99 4550089 YP_907563.1 CDS MUL_4035 NC_008611.1 4505592 4506947 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; possibly involved in the uptake of malate and other dicarboxylates by a proton symport mechanism. in E. coli, this protein has been implicated in resistance to tellurite.; C4-dicarboxylate transporter/malic acid transport protein complement(4505592..4506947) Mycobacterium ulcerans Agy99 4550090 YP_907564.1 CDS MUL_4036 NC_008611.1 4506947 4507189 D secreted protein; hypothetical protein 4506947..4507189 Mycobacterium ulcerans Agy99 4550091 YP_907565.1 CDS MUL_4038 NC_008611.1 4508056 4508760 R membrane protein; hypothetical protein complement(4508056..4508760) Mycobacterium ulcerans Agy99 4550093 YP_907566.1 CDS cpsA NC_008611.1 4509084 4510559 D membrane protein; function unknown but contains a cell envelope- related transcriptional attenuator domain. CpsA is a domain of unknown function that is found in the predicted extracellular domain of a number of putative membrane- bound proteins. may play a role in regulation of transcription.; membrane protein CpsA 4509084..4510559 Mycobacterium ulcerans Agy99 4550094 YP_907567.1 CDS MUL_4041 NC_008611.1 4510566 4511357 R cytoplasmic protein; function unknown, probably involved in cellular metabolism; short-chain type dehydrogenase/reductase complement(4510566..4511357) Mycobacterium ulcerans Agy99 4550095 YP_907568.1 CDS MUL_4042 NC_008611.1 4511361 4512194 R cytoplasmic protein; function unknown, supposed to be involved in cellular metabolism.; short chain dehydrogenase complement(4511361..4512194) Mycobacterium ulcerans Agy99 4550096 YP_907569.1 CDS MUL_4043 NC_008611.1 4512286 4512528 R cytoplasmic protein; probable role in the biosynthesis of the product from U/S PKS gene, carrying its acyl intermediates.; acyl carrier protein complement(4512286..4512528) Mycobacterium ulcerans Agy99 4550097 YP_907570.1 CDS MUL_4044 NC_008611.1 4512525 4514174 R activates fatty acids by binding to coenzyme A; long-chain-fatty-acid--CoA ligase complement(4512525..4514174) Mycobacterium ulcerans Agy99 4550098 YP_907571.1 CDS bioF2 NC_008611.1 4514315 4515505 R cytoplasmic protein; could be involved in biotin biosynthesis (at the first step) [catalytic activity: 6-carboxyhexanoyl-CoA + L- alanine = 8-amino-7-oxononanoate + CoA + CO2]; 8-amino-7-oxononanoate synthase complement(4514315..4515505) Mycobacterium ulcerans Agy99 4550099 YP_907572.1 CDS MUL_4047 NC_008611.1 4516751 4517584 R membrane protein; function unknown but domain identity suggests permease component of an uncharacterized ABC-type transport system.; hypothetical protein complement(4516751..4517584) Mycobacterium ulcerans Agy99 4550101 YP_907573.1 CDS pstB NC_008611.1 4517584 4518264 R membrane protein; involved in active transport of inorganic phosphate across the membrane (import); responsible for energy coupling to the transport system. this is one of the proteins required for binding-protein-mediated phosphate transport. have ATP-binding ability and ATPase activity.; phosphate-transport ATP-binding protein ABC transporter PstB complement(4517584..4518264) Mycobacterium ulcerans Agy99 4550102 YP_907574.1 CDS MUL_4050 NC_008611.1 4519950 4520252 D cytoplasmic protein; members of the PadR-like family are transcriptional regulators that appear to be related to the pfam01047 family. this family includes PadR, a protein that is involved in negative regulation of phenolic acid metabolism.; hypothetical protein 4519950..4520252 Mycobacterium ulcerans Agy99 4550104 YP_907575.1 CDS MUL_4051 NC_008611.1 4520320 4520484 D cytoplasmic protein; hypothetical protein 4520320..4520484 Mycobacterium ulcerans Agy99 4550105 YP_907576.1 CDS otsA NC_008611.1 4520485 4521987 D cytoplasmic protein; involved in osmoregulatory trehalose biosynthesis. mycobacteria can produce trehalose from glucose 6- phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway) [catalytic activity: trehalose 6-phosphate + H(2)O = trehalose + orthophosphate]; alpha, alpha-trehalose-phosphate synthase OtsA 4520485..4521987 Mycobacterium ulcerans Agy99 4550106 YP_907577.1 CDS MUL_4055 NC_008611.1 4522702 4523202 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; MCE-associated protein complement(4522702..4523202) Mycobacterium ulcerans Agy99 4550108 YP_907578.1 CDS MUL_4056 NC_008611.1 4523202 4523933 R membrane protein; hypothetical protein complement(4523202..4523933) Mycobacterium ulcerans Agy99 4550109 YP_907579.1 CDS mce4F NC_008611.1 4523966 4525648 R membrane protein; function unknown, but thought involved in host cell invasion.; MCE-family protein Mce4F complement(4523966..4525648) Mycobacterium ulcerans Agy99 4550110 YP_907580.1 CDS lprN NC_008611.1 4525660 4526811 R secreted protein; function unknown, but thought to be involved in host cell invasion.; MCE family lipoprotein LprN complement(4525660..4526811) Mycobacterium ulcerans Agy99 4550111 YP_907581.1 CDS mce4D NC_008611.1 4526808 4528241 R membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE-family protein Mce4D complement(4526808..4528241) Mycobacterium ulcerans Agy99 4550112 YP_907582.1 CDS mce4C NC_008611.1 4528238 4529299 R membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE-family protein Mce4C complement(4528238..4529299) Mycobacterium ulcerans Agy99 4550113 YP_907583.1 CDS mce4B NC_008611.1 4529289 4530341 R membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE-family protein Mce4B complement(4529289..4530341) Mycobacterium ulcerans Agy99 4550114 YP_907584.1 CDS mce4A NC_008611.1 4530341 4531543 R membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE-family protein Mce4A complement(4530341..4531543) Mycobacterium ulcerans Agy99 4550115 YP_907585.1 CDS yrbE4B NC_008611.1 4531559 4532401 R membrane protein; membrane protein YrbE4B complement(4531559..4532401) Mycobacterium ulcerans Agy99 4550116 YP_907586.1 CDS yrbE4A NC_008611.1 4532436 4533275 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; membrane protein YrbE4A complement(4532436..4533275) Mycobacterium ulcerans Agy99 4550117 YP_907587.1 CDS fabG NC_008611.1 4533437 4534330 R Catalyzes the first of the two reduction steps in the elongation cycle of fatty acid synthesis; 3-ketoacyl-ACP reductase complement(4533437..4534330) Mycobacterium ulcerans Agy99 4552606 YP_907588.1 CDS fdxD NC_008611.1 4534430 4534678 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the secreted fraction by proteomics.; cytoplasmic protein; ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.; ferredoxin FdxD complement(4534430..4534678) Mycobacterium ulcerans Agy99 4552607 YP_907589.1 CDS fadE26 NC_008611.1 4534843 4536021 D Detected in the membrane fraction by proteomics.; membrane protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE26 4534843..4536021 Mycobacterium ulcerans Agy99 4552608 YP_907590.1 CDS fadE27 NC_008611.1 4536052 4537149 D cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE27 4536052..4537149 Mycobacterium ulcerans Agy99 4552609 YP_907591.1 CDS fadD17 NC_008611.1 4537212 4538723 D activates fatty acids by binding to coenzyme A; acyl-CoA synthetase 4537212..4538723 Mycobacterium ulcerans Agy99 4552610 YP_907592.1 CDS MUL_4071 NC_008611.1 4540131 4540856 D function unknown but contains beta- lactamase/transpeptidase-like superfamily domain; hypothetical protein 4540131..4540856 Mycobacterium ulcerans Agy99 4552612 YP_907593.1 CDS ilvX NC_008611.1 4540853 4542400 R thiamine-pyrophosphate requiring enzyme; hypothetical protein complement(4540853..4542400) Mycobacterium ulcerans Agy99 4552613 YP_907594.1 CDS MUL_4074 NC_008611.1 4543296 4544063 R cytoplasmic protein; fructosamine kinase-like protein complement(4543296..4544063) Mycobacterium ulcerans Agy99 4552615 YP_907595.1 CDS fadD19_1 NC_008611.1 4544077 4545729 R activates fatty acids by binding to coenzyme A; in Mycobacterium may be involved in virulence; acyl-CoA synthetase complement(4544077..4545729) Mycobacterium ulcerans Agy99 4552616 YP_907596.1 CDS echA19 NC_008611.1 4545764 4546618 D Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase 4545764..4546618 Mycobacterium ulcerans Agy99 4552617 YP_907597.1 CDS cyp142A3 NC_008611.1 4546636 4547847 R cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.; cytochrome P450 142A3 Cyp142A3 complement(4546636..4547847) Mycobacterium ulcerans Agy99 4552618 YP_907598.1 CDS MUL_4078 NC_008611.1 4547871 4548581 D cytoplasmic protein; hypothetical protein 4547871..4548581 Mycobacterium ulcerans Agy99 4552619 YP_907599.1 CDS MUL_4079 NC_008611.1 4548642 4549679 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; coenzyme F420-dependent oxidoreductase complement(4548642..4549679) Mycobacterium ulcerans Agy99 4552620 YP_907600.1 CDS MUL_4080 NC_008611.1 4549750 4550739 D cytoplasmic protein; function unknown, contains a C-term predicted nucleic-acid-binding domain; hypothetical protein 4549750..4550739 Mycobacterium ulcerans Agy99 4552621 YP_907601.1 CDS ltp4 NC_008611.1 4550755 4551816 D cytoplasmic protein; function unknown, probably involved in lipid metabolism.; lipid-transfer protein 4550755..4551816 Mycobacterium ulcerans Agy99 4552622 YP_907602.1 CDS ltp3 NC_008611.1 4551816 4552994 D Catalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation; acetyl-CoA acetyltransferase 4551816..4552994 Mycobacterium ulcerans Agy99 4551358 YP_907603.1 CDS MUL_4083 NC_008611.1 4553000 4553881 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 4553000..4553881 Mycobacterium ulcerans Agy99 4551359 YP_907604.1 CDS MUL_4084 NC_008611.1 4553878 4554288 D cytoplasmic protein; function unknown, this class of proteins is involved in enzymatic cleavage of a C-H bond adjacent to carbonyl or a carboxyl group, leading to an enol or a enolate intermediate that is subsequently re-protonated at the same or an adjacent carbon.; ketosteroid isomerase- like protein 4553878..4554288 Mycobacterium ulcerans Agy99 4551360 YP_907605.1 CDS MUL_4088 NC_008611.1 4558214 4558738 R cytoplasmic protein; function unknown, contains carbonic anhydrases/acetyltransferase superfamily domain; hypothetical protein complement(4558214..4558738) Mycobacterium ulcerans Agy99 4551363 YP_907606.1 CDS MUL_4089 NC_008611.1 4558852 4560036 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 4558852..4560036 Mycobacterium ulcerans Agy99 4551364 YP_907607.1 CDS MUL_4090 NC_008611.1 4560042 4560491 D cytoplasmic protein; hypothetical protein 4560042..4560491 Mycobacterium ulcerans Agy99 4551365 YP_907608.1 CDS MUL_4091 NC_008611.1 4560495 4561640 R cytoplasmic protein; function unknown, contains P-loop containing nucleoside triphosphate hydrolase superfamily domain; hypothetical protein complement(4560495..4561640) Mycobacterium ulcerans Agy99 4551366 YP_907609.1 CDS MUL_4092 NC_008611.1 4561640 4562380 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; short chain dehydrogenase complement(4561640..4562380) Mycobacterium ulcerans Agy99 4551367 YP_907610.1 CDS MUL_4093 NC_008611.1 4562436 4563569 R cytoplasmic protein; hypothetical protein complement(4562436..4563569) Mycobacterium ulcerans Agy99 4551368 YP_907611.1 CDS gcs2 NC_008611.1 4563831 4566416 D cytoplasmic protein; function unknown, this enzyme catalyses the first and rate limiting step in de novo glutathione biosynthesis.; glutamate--cysteine ligase, Gcs2 4563831..4566416 Mycobacterium ulcerans Agy99 4551369 YP_907612.1 CDS MUL_4095 NC_008611.1 4566403 4567446 R catalyzes the formation of pyruvate and acetaldehyde from 4-hydroxy-2-ketovaleric acid; involved in the degradation of phenylpropionate; 4-hydroxy-2-ketovalerate aldolase complement(4566403..4567446) Mycobacterium ulcerans Agy99 4551370 YP_907613.1 CDS mhpF_1 NC_008611.1 4567443 4568369 R catalyzes the formation of acetyl-CoA from acetalaldehyde; acetaldehyde dehydrogenase complement(4567443..4568369) Mycobacterium ulcerans Agy99 4551371 YP_907614.1 CDS MUL_4097 NC_008611.1 4568413 4569357 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; 2-keto-4-pentenoate hydratase complement(4568413..4569357) Mycobacterium ulcerans Agy99 4551372 YP_907615.2 CDS MUL_4098 NC_008611.1 4569282 4570976 D initiates steroid ring degradation; catalyzes the transhydrogenation of 3-keto-4-ene-steroid to 3-keto-1,4-diene-steroid e.g., progesterone to 1,4-androstadiene-3,17-dione; 3-ketosteroid-delta-1-dehydrogenase 4569282..4570976 Mycobacterium ulcerans Agy99 4551373 YP_907616.1 CDS MUL_4099 NC_008611.1 4570978 4571847 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; dehydratase 4570978..4571847 Mycobacterium ulcerans Agy99 4551374 YP_907617.1 CDS MUL_4100 NC_008611.1 4572067 4572780 R cytoplasmic protein; function unknown, contains restriction endonuclease- like superfamily domain; hypothetical protein complement(4572067..4572780) Mycobacterium ulcerans Agy99 4551375 YP_907618.1 CDS ltp2_1 NC_008611.1 4572911 4574071 R cytoplasmic protein; function unknown, supposed involvement in lipid metabolism.; lipid-transfer protein complement(4572911..4574071) Mycobacterium ulcerans Agy99 4551376 YP_907619.1 CDS MUL_4102 NC_008611.1 4574071 4574457 R cytoplasmic protein; function unknown, potential role in lipid metabolism. contains thioesterase/thiol ester dehydrase- isomerase superfamily domain.; hypothetical protein complement(4574071..4574457) Mycobacterium ulcerans Agy99 4552414 YP_907620.1 CDS MUL_4103 NC_008611.1 4574469 4575419 R cytoplasmic protein; function unknown, contains a predicted C-term nucleic-acid-binding protein; hypothetical protein complement(4574469..4575419) Mycobacterium ulcerans Agy99 4552415 YP_907621.1 CDS fadE29 NC_008611.1 4575416 4576579 R Detected in the cytoplasmic fraction by proteomics.; cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE29 complement(4575416..4576579) Mycobacterium ulcerans Agy99 4552416 YP_907622.1 CDS fadE28 NC_008611.1 4576564 4577595 R cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE28 complement(4576564..4577595) Mycobacterium ulcerans Agy99 4552417 YP_907623.1 CDS cyp125A7 NC_008611.1 4577613 4578866 R cytoplasmic protein; cytochromes P450 are a group of heme-thiolate monooxygenases. they oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.; cytochrome P450 125A7 Cyp125A7 complement(4577613..4578866) Mycobacterium ulcerans Agy99 4552418 YP_907624.1 CDS fadA5 NC_008611.1 4579037 4580212 D Catalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation; acetyl-CoA acetyltransferase 4579037..4580212 Mycobacterium ulcerans Agy99 4552419 YP_907625.1 CDS MUL_4109 NC_008611.1 4580675 4581172 D cytoplasmic protein; function unknown, contains a FMN-binding split barrel domain. possible role in the electron-transfer pathway. the FMN-binding split barrel is related to the ferredoxin reductase-like FAD-binding domain. flavodoxins are an example of a group of proteins with a tightly bound flavin mononucleotide (FMN) that mediate electron transfer at low redox potential.; hypothetical protein 4580675..4581172 Mycobacterium ulcerans Agy99 4552421 YP_907626.1 CDS MUL_4110 NC_008611.1 4581193 4582098 R cytoplasmic protein; function unknown, supposed involvement in cellular metabolism.; short chain dehydrogenase complement(4581193..4582098) Mycobacterium ulcerans Agy99 4552422 YP_907627.1 CDS MUL_4111 NC_008611.1 4582119 4582898 R cytoplasmic protein; function unknown, supposed involvement in cellular metabolism.; short chain dehydrogenase complement(4582119..4582898) Mycobacterium ulcerans Agy99 4552423 YP_907628.1 CDS echA20 NC_008611.1 4585247 4585990 D Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase 4585247..4585990 Mycobacterium ulcerans Agy99 4552425 YP_907629.1 CDS MUL_4114 NC_008611.1 4585987 4586865 D cytoplasmic protein; function unknown. possible subunit of a CoA- transferase, catalyzing the reversible of CoA from one carboxylic acid to another.; CoA-transferase subunit alpha 4585987..4586865 Mycobacterium ulcerans Agy99 4552426 YP_907630.1 CDS MUL_4115 NC_008611.1 4586862 4587605 D cytoplasmic protein; function unknown. possible subunit of a CoA- transferase, catalyzing the reversible of CoA from one carboxylic acid to another.; CoA-transferase subunit beta 4586862..4587605 Mycobacterium ulcerans Agy99 4552427 YP_907631.1 CDS MUL_4116 NC_008611.1 4587602 4588669 D cytoplasmic protein; function unknown, members of this family catalyse the denitrification of a number of nitroalkanes using either fad or FMN as a cofactor.; 2-nitropropane dioxygenase 4587602..4588669 Mycobacterium ulcerans Agy99 4552428 YP_907632.1 CDS fdxB NC_008611.1 4588700 4590721 D membrane protein; function unknown, c-terminus probably involved in electron transfer in one or several metabolic reactions.; electron transfer protein FdxB 4588700..4590721 Mycobacterium ulcerans Agy99 4552429 YP_907633.1 CDS fadA6_1 NC_008611.1 4592854 4594014 R Catalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation; acetyl-CoA acetyltransferase complement(4592854..4594014) Mycobacterium ulcerans Agy99 4552431 YP_907634.1 CDS MUL_4120 NC_008611.1 4594017 4594574 R cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator complement(4594017..4594574) Mycobacterium ulcerans Agy99 4552432 YP_907635.1 CDS MUL_4122 NC_008611.1 4595302 4596348 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(4595302..4596348) Mycobacterium ulcerans Agy99 4552286 YP_907636.1 CDS MUL_4124 NC_008611.1 4597857 4598645 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; short chain dehydrogenase complement(4597857..4598645) Mycobacterium ulcerans Agy99 4552288 YP_907637.1 CDS fadE30 NC_008611.1 4598642 4599790 R cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE30 complement(4598642..4599790) Mycobacterium ulcerans Agy99 4552289 YP_907638.1 CDS fadD3 NC_008611.1 4599861 4601384 D activates fatty acids by binding to coenzyme A; long-chain-fatty-acid--CoA ligase 4599861..4601384 Mycobacterium ulcerans Agy99 4552290 YP_907639.1 CDS fadE31 NC_008611.1 4601385 4602518 D cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE31 4601385..4602518 Mycobacterium ulcerans Agy99 4552291 YP_907640.1 CDS fadE32 NC_008611.1 4602522 4603457 D cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE32 4602522..4603457 Mycobacterium ulcerans Agy99 4552292 YP_907641.1 CDS fadE33 NC_008611.1 4603507 4604469 D cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE33 4603507..4604469 Mycobacterium ulcerans Agy99 4552293 YP_907642.1 CDS aspB NC_008611.1 4604466 4605641 D catalyzes the formation of oxalozcetate and L-glutamate from L-aspartate and 2-oxoglutarate; aspartate aminotransferase 4604466..4605641 Mycobacterium ulcerans Agy99 4552294 YP_907643.1 CDS nat NC_008611.1 4605655 4606497 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; could have a role in acetylating, and hence inactivating, the antitubercular drug isoniazid [catalytic activity: acetyl-CoA + arylamine = CoA + N- acetylarylamine]; arylamine N-acetyltransferase Nat complement(4605655..4606497) Mycobacterium ulcerans Agy99 4552295 YP_907644.1 CDS MUL_4132 NC_008611.1 4606650 4607261 D cytoplasmic protein; function unknown.; hypothetical protein 4606650..4607261 Mycobacterium ulcerans Agy99 4552296 YP_907645.1 CDS dsbF NC_008611.1 4607561 4608136 D Detected in the secreted fraction by proteomics.; secreted protein; function unknown, possibly involved in thiol:disulfide interchange. contains PS00013 prokaryotic membrane lipoprotein lipid attachment site, N-term signal pepitde sequence and PS00194 thioredoxin family active site.; lipoprotein DsbF 4607561..4608136 Mycobacterium ulcerans Agy99 4552297 YP_907646.1 CDS MUL_4135 NC_008611.1 4609014 4609946 R membrane protein; function unknown, but domain identity suggests this protein may have metalloendopeptidase activity.; peptidase M48 like- protein complement(4609014..4609946) Mycobacterium ulcerans Agy99 4552299 YP_907647.1 CDS MUL_4136 NC_008611.1 4609943 4610449 R cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein complement(4609943..4610449) Mycobacterium ulcerans Agy99 4552300 YP_907648.1 CDS MUL_4137 NC_008611.1 4610470 4610877 D secreted protein; hypothetical protein 4610470..4610877 Mycobacterium ulcerans Agy99 4552301 YP_907649.1 CDS MUL_4138 NC_008611.1 4610963 4611529 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidase complement(4610963..4611529) Mycobacterium ulcerans Agy99 4552302 YP_907650.1 CDS bphC NC_008611.1 4611541 4612443 R cytoplasmic protein; involved in the degradation of biphenyl [catalytic activity: biphenyl-2,3-diol + O(2) = 2-hydroxy-6-oxo-6- phenylhexa-2,4-dienoate + H(2)O]; biphenyl-2,3-diol 1,2-dioxygenase complement(4611541..4612443) Mycobacterium ulcerans Agy99 4551843 YP_907651.1 CDS bphD NC_008611.1 4612440 4613327 R cytoplasmic protein; involved in the degradation of biphenyl.; 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase BphD complement(4612440..4613327) Mycobacterium ulcerans Agy99 4551844 YP_907652.1 CDS MUL_4141 NC_008611.1 4613327 4614511 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; acyl-CoA dehydrogenase complement(4613327..4614511) Mycobacterium ulcerans Agy99 4551845 YP_907653.1 CDS hmp NC_008611.1 4614705 4615781 D cytoplasmic protein; may play a role in protection from oxidative (nitric oxide) and nitrosative stress. may also be involved in anaerobic metabolism. could have nitric oxide dioxygenase activity.; flavodoxin reductase Hmp 4614705..4615781 Mycobacterium ulcerans Agy99 4551846 YP_907654.1 CDS MUL_4143 NC_008611.1 4615827 4616357 D secreted protein; hypothetical protein 4615827..4616357 Mycobacterium ulcerans Agy99 4551847 YP_907655.1 CDS fadE34 NC_008611.1 4616373 4618508 R cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE34 complement(4616373..4618508) Mycobacterium ulcerans Agy99 4551848 YP_907656.1 CDS MUL_4145 NC_008611.1 4618790 4619392 D cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator 4618790..4619392 Mycobacterium ulcerans Agy99 4551849 YP_907657.1 CDS MUL_4146 NC_008611.1 4619399 4620478 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; involved in transcriptional mechanism.; PurR family transcriptional regulator complement(4619399..4620478) Mycobacterium ulcerans Agy99 4551850 YP_907658.1 CDS MUL_4147 NC_008611.1 4620574 4621479 D membrane protein; function unknown, domain identity suggests periplasmic solute binding protein, containing an ATP- binding cassette transport system; hypothetical protein 4620574..4621479 Mycobacterium ulcerans Agy99 4551851 YP_907659.1 CDS MUL_4148 NC_008611.1 4621488 4622306 D membrane protein; function unknown, part of a binding-protein- dependent ABC transport system; ATPase component of an ABC-type transport system 4621488..4622306 Mycobacterium ulcerans Agy99 4551852 YP_907660.1 CDS MUL_4149 NC_008611.1 4622303 4623154 D membrane protein; part of an ABC transport system; permease of an ABC-type transporter 4622303..4623154 Mycobacterium ulcerans Agy99 4551853 YP_907661.1 CDS lppH NC_008611.1 4623241 4623957 D secreted protein; lipoprotein LppH 4623241..4623957 Mycobacterium ulcerans Agy99 4551854 YP_907662.1 CDS MUL_4151 NC_008611.1 4623972 4624721 D membrane protein; lipoprotein 4623972..4624721 Mycobacterium ulcerans Agy99 4551855 YP_907663.1 CDS lamB NC_008611.1 4624740 4625498 D cytoplasmic protein; function unknown, the lam locus of aspergillus nidulans consists of two divergently transcribed genes, lama and Lamb, involved in the utilization of lactams such as 2-pyrrolidinone. both genes are under the control of the positive regulatory gene AmdR and are subject to carbon and nitrogen metabolite repression. the exact molecular function of the proteins in this family is unknown.; LamB/YcsF family protein 4624740..4625498 Mycobacterium ulcerans Agy99 4551856 YP_907664.1 CDS MUL_4153 NC_008611.1 4625498 4626418 D cytoplasmic protein; hypothetical protein 4625498..4626418 Mycobacterium ulcerans Agy99 4551857 YP_907665.1 CDS arsB2 NC_008611.1 4626441 4627682 D membrane protein; thought to be involved in transport of arsenic across the membrane (export): arsenic resistance by an export mechanism. form the channel of an arsenite pump responsible for the translocation of the substrate across the membrane.; arsenical pump integral membrane protein ArsB2 4626441..4627682 Mycobacterium ulcerans Agy99 4551858 YP_907666.1 CDS MUL_4155 NC_008611.1 4627705 4628637 R Detected in the membrane fraction by proteomics.; cytoplasmic protein; function unknown role in tRNA and rRNA base modification by methylation.; tRNA/rRNA methyltransferase complement(4627705..4628637) Mycobacterium ulcerans Agy99 4551859 YP_907667.1 CDS cysS NC_008611.1 4628638 4630044 R catalyzes a two-step reaction; charges a cysteine by linking its carboxyl group to the alpha-phosphate of ATP then transfers the aminoacyl-adenylate to its tRNA; cysteinyl-tRNA synthetase complement(4628638..4630044) Mycobacterium ulcerans Agy99 4551860 YP_907668.1 CDS ispF NC_008611.1 4630102 4630581 R catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase complement(4630102..4630581) Mycobacterium ulcerans Agy99 4551861 YP_907669.1 CDS ispD NC_008611.1 4630596 4631279 R 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate; involved in isoprenoid and isopentenyl-PP biosynthesis; forms homodimers; 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase complement(4630596..4631279) Mycobacterium ulcerans Agy99 4551582 YP_907670.1 CDS MUL_4159 NC_008611.1 4631296 4631784 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown role in regulation; transcriptional regulatory protein complement(4631296..4631784) Mycobacterium ulcerans Agy99 4551583 YP_907671.1 CDS lpqE NC_008611.1 4632227 4632784 D membrane protein; lipoprotein LpqE 4632227..4632784 Mycobacterium ulcerans Agy99 4551584 YP_907672.1 CDS MUL_4162 NC_008611.1 4634325 4635401 D non-specific DNA-binding; scans chromosomes during sporulation for DNA-damage; delays initiation of sporulation; participates in a checkpoint signaling cascade for cell-cycle progression and DNA repair; DNA integrity scanning protein DisA 4634325..4635401 Mycobacterium ulcerans Agy99 4551586 YP_907673.1 CDS MUL_4163 NC_008611.1 4635413 4636234 R Detected in the membrane fraction by proteomics (LC- MS/MS) Also detected in the secreted protein fraction; membrane protein; hypothetical protein complement(4635413..4636234) Mycobacterium ulcerans Agy99 4551587 YP_907674.1 CDS MUL_4164 NC_008611.1 4636324 4636941 R cytoplasmic protein; catalyzes the reversible hydratation of carbon dioxide [catalytic activity: H(2)CO(3) = CO(2) + H(2)O]; carbonic anhydrase complement(4636324..4636941) Mycobacterium ulcerans Agy99 4551588 YP_907675.1 CDS mutY NC_008611.1 4636940 4637851 D cytoplasmic protein; involved in base excision repair. removes adenine mispaired with 8-OXOG. may repair a.G and a.C mismatches by adenine excision.; adenine glycosylase MutY 4636940..4637851 Mycobacterium ulcerans Agy99 4551589 YP_907676.1 CDS MUL_4166 NC_008611.1 4637859 4638626 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; hydrolase complement(4637859..4638626) Mycobacterium ulcerans Agy99 4551590 YP_907677.1 CDS lpqF NC_008611.1 4639031 4640344 D membrane protein; lipoprotein LpqF 4639031..4640344 Mycobacterium ulcerans Agy99 4551592 YP_907678.1 CDS ppm1_2 NC_008611.1 4640202 4641164 R cytoplasmic protein; transfers mannose from GDP-mannose to all endogenous polyprenol-phosphates.; polyprenol-monophosphomannose synthase Ppm1_2 complement(4640202..4641164) Mycobacterium ulcerans Agy99 4551593 YP_907679.1 CDS MUL_4170 NC_008611.1 4641161 4642729 R membrane protein; thought to be involved in a transport system across the membrane (perhaps drug transport): responsible for the translocation of the substrate across the membrane.; integral membrane transport protein complement(4641161..4642729) Mycobacterium ulcerans Agy99 4551594 YP_907680.1 CDS gpmB NC_008611.1 4644060 4644593 R cytoplasmic protein; may be involved in the catalysis of both the synthesis and the degradation of fructose-2,6- bisphosphate. beta-D-fructose 2,6-bisphosphate + H(2)O <=> D-fructose 6-phosphate + phosphate.; fructose-2,6-bisphosphatase GpmB complement(4644060..4644593) Mycobacterium ulcerans Agy99 4551596 YP_907681.1 CDS MUL_4173 NC_008611.1 4644601 4645368 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(4644601..4645368) Mycobacterium ulcerans Agy99 4551597 YP_907682.1 CDS MUL_4174 NC_008611.1 4645383 4645598 R membrane protein; hypothetical protein complement(4645383..4645598) Mycobacterium ulcerans Agy99 4551598 YP_907683.1 CDS MUL_4176 NC_008611.1 4646777 4647823 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(4646777..4647823) Mycobacterium ulcerans Agy99 4549741 YP_907684.1 CDS clpC1 NC_008611.1 4648646 4651195 R Also detected in the extracellular matrix by proteomics.; cytoplasmic protein; hydrolyses proteins in presence of ATP. may interact with a ClpP-like protease involved in degradation of denatured proteins.; ATP-dependent protease ATP-binding subunit ClpC1 complement(4648646..4651195) Mycobacterium ulcerans Agy99 4549743 YP_907685.1 CDS MUL_4179 NC_008611.1 4651370 4652416 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(4651370..4652416) Mycobacterium ulcerans Agy99 4549744 YP_907686.1 CDS lsr2 NC_008611.1 4652949 4653290 R Detected in the cytoplasmic and the extracellular matrix fractions by proteomics.; extracellular matrix protein; dominant T-cell antigen and may stimulate lymphocyte proliferation.; iron-regulated Lsr2 protein complement(4652949..4653290) Mycobacterium ulcerans Agy99 4549745 YP_907687.1 CDS lysS NC_008611.1 4653405 4654901 R class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA molecules for lysine that exist in this organism and in addition can charge the tRNAPyl with lysine in the presence of LysRS1; lysyl-tRNA synthetase complement(4653405..4654901) Mycobacterium ulcerans Agy99 4549746 YP_907688.1 CDS MUL_4182 NC_008611.1 4655023 4655841 R type III; catalyzes the formation of (R)-4'-phosphopantothenate from (R)-pantothenate in coenzyme A biosynthesis; type III pantothenate kinases are not subject to feedback inhibition from coenzyme A and have a high Km for ATP; pantothenate kinase complement(4655023..4655841) Mycobacterium ulcerans Agy99 4549747 YP_907689.1 CDS panD NC_008611.1 4655844 4656263 R Converts L-aspartate to beta-alanine and provides the major route of beta-alanine production in bacteria. Beta-alanine is essential for the biosynthesis of pantothenate (vitamin B5); aspartate alpha-decarboxylase complement(4655844..4656263) Mycobacterium ulcerans Agy99 4549748 YP_907690.1 CDS panC NC_008611.1 4656275 4657222 R catalyzes the formation of (R)-pantothenate from pantoate and beta-alanine; pantoate--beta-alanine ligase complement(4656275..4657222) Mycobacterium ulcerans Agy99 4549749 YP_907691.1 CDS MUL_4185 NC_008611.1 4657219 4658130 R function unknown contains NAD(P)-binding Rossmann- fold superfamily domains; hypothetical protein complement(4657219..4658130) Mycobacterium ulcerans Agy99 4549750 YP_907692.1 CDS MUL_4186 NC_008611.1 4658233 4659585 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; transmembrane protein rich in alanine, arginine and proline complement(4658233..4659585) Mycobacterium ulcerans Agy99 4549751 YP_907693.1 CDS MUL_4187 NC_008611.1 4659697 4660173 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(4659697..4660173) Mycobacterium ulcerans Agy99 4549752 YP_907694.1 CDS folK NC_008611.1 4660173 4660700 R cytoplasmic protein; involved in dihydrofolate biosynthesis (at the first step) [catalytic activity: ATP + 2-amino-4-hydroxy-6- hydroxymethyl-7,8-dihydropteridine = AMP + 2-amino-7,8- dihydro-4-hydroxy-6-(diphosphooxymethyl)pteri di ne]; 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase complement(4660173..4660700) Mycobacterium ulcerans Agy99 4549753 YP_907695.1 CDS folB NC_008611.1 4660697 4661098 R cytoplasmic protein; involved in folate biosynthesis. catalyzes the conversion of 7,8-dihydroneopterin to 6- hydroxymethyl-7,8- dihydropterin (by similarity) [catalytic activity: 2-amino- 4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7, 8- dihydropteridine = 2-amino-4-hydroxy-6-hydroxymethyl-7,8- dihydropteridine + glycolaldehyde]; dihydroneopterin aldolase FolB complement(4660697..4661098) Mycobacterium ulcerans Agy99 4549754 YP_907696.1 CDS folP1 NC_008611.1 4661091 4661909 R cytoplasmic protein; involved in dihydrofolate biosynthesis (at the second step) catalyzes the formation of the immediate precursor of folic acid. it is implicated in resistance to sulfonamide [catalytic activity: 2-amino-4-hydroxy-6- hydroxymethyl-7,8-dihydropteridine diphosphate + 4- aminobenzoate = pyrophosphate + dihydropteroate]; dihydropteroate synthase 1 FolP1 complement(4661091..4661909) Mycobacterium ulcerans Agy99 4549755 YP_907697.1 CDS folE NC_008611.1 4661930 4662538 R involved in the first step of tetrahydrofolate biosynthesis; catalyzes the formation of formate and 2-amino-4-hydroxy-6-(erythro-1,2, 3-trihydroxypropyl)dihydropteridine triphosphate from GTP and water; forms a homopolymer; GTP cyclohydrolase I complement(4661930..4662538) Mycobacterium ulcerans Agy99 4549756 YP_907698.1 CDS ftsH NC_008611.1 4662555 4664777 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (LC-MS/MS); membrane protein; thought to act as an ATP-dependent zinc metallopeptidase, with ATPase and proteolytic activities. probably has a regulatory role in stress response and specific proteins secretion for adaptation to host environment.; membrane-bound protease FtsH complement(4662555..4664777) Mycobacterium ulcerans Agy99 4549757 YP_907699.1 CDS ephA NC_008611.1 4665017 4665985 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; biotransformation enzyme that catalyzes the hydrolysis of epoxides (alkene oxides, oxiranes) and Arene oxides to less reactive and more water soluble dihydrodiols by the trans addition of water. thought to be involved in detoxification reactions following oxidative damage to lipids [catalytic activity: an epoxide + H(2)O = a glycol]; epoxide hydrolase EphA 4665017..4665985 Mycobacterium ulcerans Agy99 4550909 YP_907700.1 CDS MUL_4194 NC_008611.1 4665979 4667661 D potential pseudogene as C-term extension caused by removal of stop codon and additional 170 aa added.; cytoplasmic protein; monooxygenase 4665979..4667661 Mycobacterium ulcerans Agy99 4550910 YP_907701.1 CDS MUL_4195 NC_008611.1 4667212 4668468 R PPE65; membrane protein; PPE family protein complement(4667212..4668468) Mycobacterium ulcerans Agy99 4550911 YP_907702.1 CDS MUL_4196 NC_008611.1 4668481 4668780 R PE32; membrane protein; PE family protein complement(4668481..4668780) Mycobacterium ulcerans Agy99 4550912 YP_907703.1 CDS MUL_4197 NC_008611.1 4669091 4670137 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 4669091..4670137 Mycobacterium ulcerans Agy99 4550913 YP_907704.1 CDS lpqG NC_008611.1 4670831 4671577 D membrane protein; lipoprotein LpqG 4670831..4671577 Mycobacterium ulcerans Agy99 4550914 YP_907705.1 CDS hpt NC_008611.1 4671603 4672211 R cytoplasmic protein; involved in purine salvage [catalytic activity: imp + pyrophosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate (guanine can replace hypoxanthine to produce GMP)]; hypoxanthine-guanine phosphoribosyltransferase Hpt complement(4671603..4672211) Mycobacterium ulcerans Agy99 4550915 YP_907706.1 CDS mesJ NC_008611.1 4672265 4673236 R cytoplasmic protein; predicted ATPase of the PP-loop superfamily implicated in cell cycle control; cell cycle protein MesJ complement(4672265..4673236) Mycobacterium ulcerans Agy99 4550916 YP_907707.1 CDS MUL_4202 NC_008611.1 4673215 4674273 R cytoplasmic protein; hypothetical protein complement(4673215..4674273) Mycobacterium ulcerans Agy99 4550917 YP_907708.1 CDS dacB1 NC_008611.1 4674270 4675655 R membrane protein; involved in peptidoglycan synthesis (at final stages) hydrolyzes the bound D-alanyl-D-alanine [catalytic activity: D-alanyl-D-alanine + H(2)O = 2 D- alanine]; D-alanyl-D-alanine carboxypeptidase complement(4674270..4675655) Mycobacterium ulcerans Agy99 4550918 YP_907709.1 CDS ppa NC_008611.1 4675796 4676284 D Also detected in the extracellular matrix by proteomics.; cytoplasmic protein; involved in the function of cellular bioenergetics [catalytic activity: pyrophosphate + H(2)O = 2 orthophosphate]; inorganic pyrophosphatase Ppa 4675796..4676284 Mycobacterium ulcerans Agy99 4550919 YP_907710.1 CDS MUL_4205 NC_008611.1 4676293 4677369 R membrane protein; hypothetical protein complement(4676293..4677369) Mycobacterium ulcerans Agy99 4550920 YP_907711.1 CDS MUL_4206 NC_008611.1 4677492 4678826 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(4677492..4678826) Mycobacterium ulcerans Agy99 4550921 YP_907712.1 CDS MUL_4207 NC_008611.1 4679046 4680254 D membrane protein; hypothetical protein 4679046..4680254 Mycobacterium ulcerans Agy99 4550922 YP_907713.1 CDS MUL_4208 NC_008611.1 4680290 4681003 D cytoplasmic protein; function unknown, the domain detected in this CDS is found in a diverse family of glycosyl transferases that transfer the sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.; glycosyl transferase family protein 4680290..4681003 Mycobacterium ulcerans Agy99 4550923 YP_907714.1 CDS MUL_4209 NC_008611.1 4681000 4681341 D membrane protein; hypothetical protein 4681000..4681341 Mycobacterium ulcerans Agy99 4550924 YP_907715.1 CDS galE1 NC_008611.1 4681357 4682301 R cytoplasmic protein; involved in galactofuranosyl biosynthesis: converts UDO-GlcP to UDP-GalP [catalytic activity: UDP- glucopyranose = UDP-galactopyranose]; UDP-glucose 4-epimerase GalE1 complement(4681357..4682301) Mycobacterium ulcerans Agy99 4550925 YP_907716.1 CDS MUL_4211 NC_008611.1 4682334 4684118 D membrane protein; hypothetical protein 4682334..4684118 Mycobacterium ulcerans Agy99 4550926 YP_907717.1 CDS MUL_4213 NC_008611.1 4686563 4687474 R cytoplasmic protein; hydrolase complement(4686563..4687474) Mycobacterium ulcerans Agy99 4551224 YP_907718.1 CDS MUL_4214 NC_008611.1 4687699 4689033 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 4687699..4689033 Mycobacterium ulcerans Agy99 4551225 YP_907719.1 CDS MUL_4216 NC_008611.1 4689608 4689850 R cytoplasmic protein; hypothetical protein complement(4689608..4689850) Mycobacterium ulcerans Agy99 4551227 YP_907720.1 CDS MUL_4217 NC_008611.1 4690163 4690417 R cytoplasmic protein; hypothetical protein complement(4690163..4690417) Mycobacterium ulcerans Agy99 4551228 YP_907721.1 CDS MUL_4218 NC_008611.1 4690573 4690728 D cytoplasmic protein; hypothetical protein 4690573..4690728 Mycobacterium ulcerans Agy99 4551229 YP_907722.1 CDS MUL_4220 NC_008611.1 4690966 4692171 R catalyzes the DNA-template-directed extension of the 3'-end of a DNA strand; the delta' subunit seems to interact with the gamma subunit to transfer the beta subunit on the DNA; DNA polymerase III subunit delta' complement(4690966..4692171) Mycobacterium ulcerans Agy99 4551231 YP_907723.1 CDS MUL_4221 NC_008611.1 4692327 4693916 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; C-term contains an adenylyl/guanylyl cyclase domain. may catalyze the formation of cyclic AMP/GMP from ATP/GTP.; adenylate cyclase 4692327..4693916 Mycobacterium ulcerans Agy99 4551232 YP_907724.1 CDS topA NC_008611.1 4693927 4696731 R catalyzes the ATP-dependent breakage of single-stranded DNA followed by passage and rejoining, maintains net negative superhelicity; DNA topoisomerase I complement(4693927..4696731) Mycobacterium ulcerans Agy99 4551233 YP_907725.1 CDS MUL_4223 NC_008611.1 4696935 4697513 R cytoplasmic protein; hypothetical protein complement(4696935..4697513) Mycobacterium ulcerans Agy99 4551234 YP_907726.1 CDS cspA_1 NC_008611.1 4697658 4697861 R Detected in the cytoplamic and the secreted fractions by proteomics.; cytoplasmic protein; possibly involved in cold acclimatization processes (the production of the protein is supposed predominantly induced at low temperatures); cold-shock protein complement(4697658..4697861) Mycobacterium ulcerans Agy99 4551235 YP_907727.1 CDS MUL_4226 NC_008611.1 4700882 4701913 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein 4700882..4701913 Mycobacterium ulcerans Agy99 4551237 YP_907728.1 CDS MUL_4228 NC_008611.1 4702275 4702541 R cytoplasmic protein; hypothetical protein complement(4702275..4702541) Mycobacterium ulcerans Agy99 4551147 YP_907729.1 CDS MUL_4229 NC_008611.1 4702598 4702798 R membrane protein; hypothetical protein complement(4702598..4702798) Mycobacterium ulcerans Agy99 4551148 YP_907730.1 CDS MUL_4230 NC_008611.1 4702823 4703425 R membrane protein; hypothetical protein complement(4702823..4703425) Mycobacterium ulcerans Agy99 4551149 YP_907731.1 CDS MUL_4231 NC_008611.1 4703459 4704253 R membrane protein; function unknown, contains domain identity to Flp pilus assembly protein TadB; hypothetical protein complement(4703459..4704253) Mycobacterium ulcerans Agy99 4551150 YP_907732.1 CDS MUL_4232 NC_008611.1 4704250 4705419 R cytoplasmic protein; function unknown, contains domain identity with Flp pilus assembly protein, ATPase CpaF; hypothetical protein complement(4704250..4705419) Mycobacterium ulcerans Agy99 4551151 YP_907733.1 CDS MUL_4233 NC_008611.1 4705530 4706525 R cytoplasmic protein; function unknown, has a P-loop containing nucleoside triphosphate hydrolase superfamily domain.; hypothetical protein complement(4705530..4706525) Mycobacterium ulcerans Agy99 4551152 YP_907734.1 CDS serB NC_008611.1 4707003 4707860 D membrane protein; phosphoserine + H(2)O <=> serine + phosphate; phosphoserine phosphatase SerB 4707003..4707860 Mycobacterium ulcerans Agy99 4551153 YP_907735.1 CDS MUL_4235 NC_008611.1 4707986 4708300 R cytoplasmic protein; hypothetical protein complement(4707986..4708300) Mycobacterium ulcerans Agy99 4551154 YP_907736.1 CDS MUL_4236 NC_008611.1 4708353 4709123 R cytoplasmic protein; hypothetical protein complement(4708353..4709123) Mycobacterium ulcerans Agy99 4551155 YP_907737.1 CDS dppD NC_008611.1 4709135 4710790 R membrane protein; involved in active transport of dipeptides across the membrane (import) responsible for energy coupling to the transport system.; dipeptide-transport ATP-binding protein ABC transporter DppD complement(4709135..4710790) Mycobacterium ulcerans Agy99 4551156 YP_907738.1 CDS dppC NC_008611.1 4710787 4711650 R membrane protein; involved in active transport of dipeptide across the membrane (import) responsible for the translocation of the substrate across the membrane.; dipeptide-transport integral membrane protein ABC transporter DppC complement(4710787..4711650) Mycobacterium ulcerans Agy99 4551157 YP_907739.1 CDS dppB NC_008611.1 4711643 4712569 R membrane protein; involved in active transport of dipeptide across the membrane (import) responsible for the translocation of the substrate across the membrane.; dipeptide-transport integral membrane protein ABC transporter DppB complement(4711643..4712569) Mycobacterium ulcerans Agy99 4551158 YP_907740.1 CDS dppA NC_008611.1 4712571 4714328 R membrane protein; thought to be involved in active transport of dipeptide across the membrane (import); periplasmic dipeptide-binding lipoprotein DppA complement(4712571..4714328) Mycobacterium ulcerans Agy99 4551159 YP_907741.1 CDS acs NC_008611.1 4714374 4716356 D Acs; catalyzes the conversion of acetate and CoA to acetyl-CoA; acetyl-CoA synthetase 4714374..4716356 Mycobacterium ulcerans Agy99 4551160 YP_907742.1 CDS MUL_4242 NC_008611.1 4716365 4717063 R membrane protein; function unknown, domain identity suggests peptidase, trypsin-like serine or cysteine protease. contains peptidase S1B, active site.; hypothetical protein complement(4716365..4717063) Mycobacterium ulcerans Agy99 4551161 YP_907743.1 CDS MUL_4243 NC_008611.1 4717289 4717807 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 4717289..4717807 Mycobacterium ulcerans Agy99 4551162 YP_907744.1 CDS ephE NC_008611.1 4717808 4718773 D thought to be involved in detoxification reactions following oxidative damage to lipids. biotransformation enzyme that catalyzes the hydrolysis of epoxides: aromatic hydrocarbons catabolism [catalytic activity: an epoxide + H(2)O = a glycol]; epoxide hydrolase EphE 4717808..4718773 Mycobacterium ulcerans Agy99 4551163 YP_907745.1 CDS MUL_4245 NC_008611.1 4718766 4719968 R membrane protein; function unknown, hydrolyses of peptides and/or proteins (possibly cleaved preferentially after serine residue) contains peptidase S1B, active site; membrane-associated serine protease complement(4718766..4719968) Mycobacterium ulcerans Agy99 4551164 YP_907746.1 CDS MUL_4247 NC_008611.1 4720778 4721383 R membrane protein; function unknown, contains a N-term thioredoxin domain. may serve as a general protein disulphide oxidoreductase.; membrane-anchored thioredoxin-like protein complement(4720778..4721383) Mycobacterium ulcerans Agy99 4551238 YP_907747.1 CDS nth NC_008611.1 4721442 4722143 R cytoplasmic protein; has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. incises damaged DNA at cytosines, thymines and guanines. acts on a damaged strand (oxidized pyrimidines), 5' from the damaged site [catalytic activity: endonucleolytic cleavage near apurinic or apyrimidinic sites to products with 5'-phosphate]; endonuclease III Nth complement(4721442..4722143) Mycobacterium ulcerans Agy99 4551239 YP_907748.1 CDS MUL_4249 NC_008611.1 4722311 4722664 D membrane protein; hypothetical protein 4722311..4722664 Mycobacterium ulcerans Agy99 4551240 YP_907749.1 CDS MUL_4250 NC_008611.1 4722763 4723437 D Detected in the cytoplasmic and membrane fractions by proteomics.; cytoplasmic protein; involved in transcriptional mechanism.; regulatory protein 4722763..4723437 Mycobacterium ulcerans Agy99 4551241 YP_907750.1 CDS MUL_4251 NC_008611.1 4723434 4724240 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; hypothetical protein complement(4723434..4724240) Mycobacterium ulcerans Agy99 4551242 YP_907751.1 CDS MUL_4252 NC_008611.1 4724246 4724701 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown, this endoribonuclease domain is active on single-stranded mRNA and inhibits protein synthesis by cleavage of mRNA.; hypothetical protein complement(4724246..4724701) Mycobacterium ulcerans Agy99 4551243 YP_907752.1 CDS MUL_4253 NC_008611.1 4724723 4724884 R hypothetical protein complement(4724723..4724884) Mycobacterium ulcerans Agy99 4551244 YP_907753.1 CDS MUL_4254 NC_008611.1 4724961 4725983 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; function unknown, may catalyze the extrusion of undeterminated anions [catalytic activity: ATP + H(2)O + undeterminated anion(in) = ADP + phosphate + undeterminated anion(out)]; anion transporter ATPase 4724961..4725983 Mycobacterium ulcerans Agy99 4551245 YP_907754.1 CDS MUL_4255 NC_008611.1 4725980 4727140 D membrane protein; anion-transporting ATPase; supposed catalyzes the extrusion of undeterminated anions [catalytic activity: ATP + H(2)O + undeterminated anion(in) = ADP + phosphate + undeterminated anion(out)]; anion transporter ATPase 4725980..4727140 Mycobacterium ulcerans Agy99 4551246 YP_907755.1 CDS whiB4 NC_008611.1 4727288 4727470 R potential pseudogene as N-term truncated due to mutation of original start codon; cytoplasmic protein; involved in a transcriptional mechanism.; transcriptional regulatory protein Whib-like WhiB4 complement(4727288..4727470) Mycobacterium ulcerans Agy99 4551247 YP_907756.1 CDS ponA2 NC_008611.1 4727999 4730410 D membrane protein; involved in peptidoglycan synthesis (at the final stages), cell wall formation. synthesis of cross-linked peptidoglycan from the lipid intermediates. the enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits); bifunctional membrane-associated penicillin- binding protein 1A/1B PonA2 4727999..4730410 Mycobacterium ulcerans Agy99 4551248 YP_907757.1 CDS MUL_4258 NC_008611.1 4730488 4731501 D Detected in the cytoplasmic and membrane fractions by proteomics; membrane protein; function unknown, contains metallophosphoesterase domain; hypothetical protein 4730488..4731501 Mycobacterium ulcerans Agy99 4551249 YP_907758.1 CDS cysK NC_008611.1 4731498 4732604 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown, probably involved in cellular metabolism. reaction catalysed: O(3)-acetyl-L-serine + H(2)S <=> L-cysteine + acetate.; cysteine synthase 4731498..4732604 Mycobacterium ulcerans Agy99 4551250 YP_907759.1 CDS MUL_4261 NC_008611.1 4732775 4733026 R cytoplasmic protein; hypothetical protein complement(4732775..4733026) Mycobacterium ulcerans Agy99 4551252 YP_907760.1 CDS MUL_4263 NC_008611.1 4734598 4734840 R cytoplasmic protein; hypothetical protein complement(4734598..4734840) Mycobacterium ulcerans Agy99 4551254 YP_907761.1 CDS MUL_4264 NC_008611.1 4734932 4735192 R membrane protein; hypothetical protein complement(4734932..4735192) Mycobacterium ulcerans Agy99 4551255 YP_907762.1 CDS nrfD NC_008611.1 4740967 4741938 D membrane protein; NrfD is an integral transmembrane protein with loops in both the periplasm and the cytoplasm. NrfD is thought to participate in the transfer of electrons, from the quinone pool into the terminal components of the Nrf pathway.; formate-dependent nitrite reductase, membrane component, NrfD 4740967..4741938 Mycobacterium ulcerans Agy99 4551115 YP_907763.1 CDS MUL_4271 NC_008611.1 4741949 4742368 R cytoplasmic protein; function unknown, contains GatB/Yqey domain. this domain is found at the C terminus of GatB which transamidates glu-tRNA to Gln-tRNA.; hypothetical protein complement(4741949..4742368) Mycobacterium ulcerans Agy99 4551116 YP_907764.1 CDS MUL_4272 NC_008611.1 4742531 4743790 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 4742531..4743790 Mycobacterium ulcerans Agy99 4551117 YP_907765.1 CDS MUL_4273 NC_008611.1 4743811 4744461 D cytoplasmic protein; hypothetical protein 4743811..4744461 Mycobacterium ulcerans Agy99 4551118 YP_907766.1 CDS MUL_4274 NC_008611.1 4744461 4745600 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein 4744461..4745600 Mycobacterium ulcerans Agy99 4551119 YP_907767.1 CDS moxR2 NC_008611.1 4745597 4746574 D Detected in the cytoplasmic fraction by LC-MS/MS. Also detected in the extracellular matrix and membrane fraction by proteomics.; cytoplasmic protein; involved in transcriptional mechanism; regulates methanol dehydrogenase.; methanol dehydrogenase transcriptional regulatory protein MoxR2 4745597..4746574 Mycobacterium ulcerans Agy99 4551120 YP_907768.1 CDS MUL_4276 NC_008611.1 4746574 4747896 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; function unknown, contains N-term signal peptide; hypothetical protein 4746574..4747896 Mycobacterium ulcerans Agy99 4551121 YP_907769.1 CDS MUL_4277 NC_008611.1 4747901 4748893 R membrane protein; hypothetical protein complement(4747901..4748893) Mycobacterium ulcerans Agy99 4551122 YP_907770.1 CDS MUL_4278 NC_008611.1 4748998 4749903 D membrane protein; function unknown, contains RDD domain RDD family. the molecular function of this region is unknown. however this region may be involved in transport of an as yet unknown set of ligands.; hypothetical protein 4748998..4749903 Mycobacterium ulcerans Agy99 4551123 YP_907771.1 CDS MUL_4279 NC_008611.1 4749974 4750741 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; PadR-like transcriptional regulatory protein 4749974..4750741 Mycobacterium ulcerans Agy99 4551124 YP_907772.1 CDS MUL_4280 NC_008611.1 4750900 4753086 D PE_PGRS9_2; membrane protein; PE-PGRS family protein 4750900..4753086 Mycobacterium ulcerans Agy99 4551125 YP_907773.1 CDS glpK NC_008611.1 4753083 4754609 R Converts glycerol and ADP to glycerol-3-phosphate and ADP; glycerol kinase complement(4753083..4754609) Mycobacterium ulcerans Agy99 4551126 YP_907774.1 CDS csdB NC_008611.1 4756949 4758100 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; probable role in the metabolism of proteins containing the rare amino acid selenocysteine; selenocysteine lyase, CsdB complement(4756949..4758100) Mycobacterium ulcerans Agy99 4551094 YP_907775.1 CDS MUL_4285 NC_008611.1 4758097 4759062 R cytoplasmic protein; S-adenosyl-L-methionine- dependentmethyltransferase complement(4758097..4759062) Mycobacterium ulcerans Agy99 4551095 YP_907776.1 CDS MUL_4286 NC_008611.1 4759059 4759772 R cytoplasmic protein; function unknown, contains N-terminal nucleophile aminohydrolase superfamily domain; hypothetical protein complement(4759059..4759772) Mycobacterium ulcerans Agy99 4551096 YP_907777.1 CDS MUL_4287 NC_008611.1 4759772 4761073 R cytoplasmic protein; hypothetical protein complement(4759772..4761073) Mycobacterium ulcerans Agy99 4551097 YP_907778.1 CDS MUL_4290 NC_008611.1 4762505 4763203 R secreted protein; hypothetical protein complement(4762505..4763203) Mycobacterium ulcerans Agy99 4551099 YP_907779.1 CDS katE NC_008611.1 4763200 4764648 R Also detected in the membrane fraction by proteomics (LC-MS/MS); cytoplasmic protein; protects against oxidative stress. converts hydrogen peroxide to water and molecular oxygen.; catalase KatE complement(4763200..4764648) Mycobacterium ulcerans Agy99 4551100 YP_907780.1 CDS MUL_4292 NC_008611.1 4764793 4765167 R cytoplasmic protein; function unknown, rich in short repeat motifs.; hypothetical protein complement(4764793..4765167) Mycobacterium ulcerans Agy99 4551101 YP_907781.1 CDS MUL_4293 NC_008611.1 4765358 4765705 R cytoplasmic protein; function unknown, rich in short repeat motifs.; hypothetical protein complement(4765358..4765705) Mycobacterium ulcerans Agy99 4551102 YP_907782.1 CDS MUL_4295 NC_008611.1 4766922 4768058 R secreted protein; hypothetical protein complement(4766922..4768058) Mycobacterium ulcerans Agy99 4551104 YP_907783.1 CDS asd NC_008611.1 4768066 4769124 R catalyzes the formation of 4-aspartyl phosphate from aspartate 4-semialdehyde; aspartate-semialdehyde dehydrogenase complement(4768066..4769124) Mycobacterium ulcerans Agy99 4551105 YP_907784.1 CDS ask NC_008611.1 4769126 4770391 R catalyzes the formation of 4-phospho-L-aspartate from L-aspartate and ATP, in Bacillus, lysine sensitive; regulated by response to starvation.; aspartate kinase complement(4769126..4770391) Mycobacterium ulcerans Agy99 4551106 YP_907785.1 CDS MUL_4299 NC_008611.1 4774356 4776248 R cytoplasmic protein; hypothetical protein complement(4774356..4776248) Mycobacterium ulcerans Agy99 4551108 YP_907786.1 CDS leuA NC_008611.1 4776991 4778691 D cytoplasmic protein; involved in leucine biosynthesis (at the first step) catalyzes condensation of acetyl-CoA and 2- oxoisovalerate to form 2-isopropylmalate synthase [catalytic activity: 3-carboxy-3-hydroxy-4- methylpentanoate + CoA = acetyl-CoA + 3-methyl-2- oxobutanoate + H(2)O]; 2-isopropylmalate synthase 4776991..4778691 Mycobacterium ulcerans Agy99 4551109 YP_907787.1 CDS MUL_4301 NC_008611.1 4778688 4779251 R cytoplasmic protein; involved in transcriptional mechanism.; DeoR family transcriptional regulator complement(4778688..4779251) Mycobacterium ulcerans Agy99 4551304 YP_907788.1 CDS MUL_4302 NC_008611.1 4779251 4779616 R membrane protein; function unknown, contains multidrug efflux transporter AcrB transmembrane superfamily domain; hypothetical protein complement(4779251..4779616) Mycobacterium ulcerans Agy99 4551305 YP_907789.1 CDS dnaQ NC_008611.1 4779747 4780736 R 3'-5' exonuclease of DNA polymerase III; DNA polymerase III subunit epsilon complement(4779747..4780736) Mycobacterium ulcerans Agy99 4551306 YP_907790.1 CDS MUL_4304 NC_008611.1 4780969 4782123 D membrane protein; possible role in cell wall peptidoglycan biogenesis. contains Mur ligase family catalytic domain. possible UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D- alanine ligase, required for the formation of the UDP- MurNAc-linked pentapeptide by addition of the D-Ala-D-Ala subgroup.; UDP-N-acetylmuramyl tripeptide synthase 4780969..4782123 Mycobacterium ulcerans Agy99 4551307 YP_907791.1 CDS cobQ2 NC_008611.1 4782116 4782823 D cytoplasmic protein; involved in cobalamin biosynthesis. catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic a,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation (by similarity); cobyric acid synthase CobQ2 4782116..4782823 Mycobacterium ulcerans Agy99 4551308 YP_907792.1 CDS recR NC_008611.1 4782839 4783450 R involved in a recombinational process of DNA repair, independent of the recBC complex; recombination protein RecR complement(4782839..4783450) Mycobacterium ulcerans Agy99 4551309 YP_907793.1 CDS MUL_4307 NC_008611.1 4783462 4783905 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(4783462..4783905) Mycobacterium ulcerans Agy99 4551310 YP_907794.1 CDS MUL_4308 NC_008611.1 4784040 4784765 D membrane protein; hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain bacterial cell-wall glycopeptides.; N-acetylmuramoyl-L-alanine amidase 4784040..4784765 Mycobacterium ulcerans Agy99 4551311 YP_907795.1 CDS MUL_4309 NC_008611.1 4784762 4785220 R Detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; function unknown, contains bet v1-like superfamily domain; hypothetical protein complement(4784762..4785220) Mycobacterium ulcerans Agy99 4551312 YP_907796.1 CDS MUL_4310 NC_008611.1 4785333 4786658 D cytoplasmic protein; dehydrogenase 4785333..4786658 Mycobacterium ulcerans Agy99 4551313 YP_907797.1 CDS MUL_4312 NC_008611.1 4787982 4788224 D membrane protein; hypothetical protein 4787982..4788224 Mycobacterium ulcerans Agy99 4551315 YP_907798.1 CDS MUL_4313 NC_008611.1 4788221 4788766 D cytoplasmic protein; hypothetical protein 4788221..4788766 Mycobacterium ulcerans Agy99 4551316 YP_907799.1 CDS MUL_4314 NC_008611.1 4788763 4789134 D cytoplasmic protein; hypothetical protein 4788763..4789134 Mycobacterium ulcerans Agy99 4551317 YP_907800.1 CDS dnaZX NC_008611.1 4789288 4791153 R catalyzes the DNA-template-directed extension of the 3'-end of a DNA strand; the tau chain serves as a scaffold to help in the dimerizaton of the alpha,epsilon and theta core complex; the gamma chain seems to interact with the delta and delta' subunits to transfer the beta subunit on the DNA; DNA polymerase III subunits gamma and tau complement(4789288..4791153) Mycobacterium ulcerans Agy99 4551318 YP_907801.1 CDS MUL_4316 NC_008611.1 4791212 4792498 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the extracellular matrix and the membrane fractions by proteomics.; cytoplasmic protein; transcriptional regulator complement(4791212..4792498) Mycobacterium ulcerans Agy99 4551319 YP_907802.1 CDS MUL_4318 NC_008611.1 4792943 4793758 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 4792943..4793758 Mycobacterium ulcerans Agy99 4551321 YP_907803.1 CDS echA3_1 NC_008611.1 4793790 4794551 R Detected in the cytoplasmic fraction by proteomics; cytoplasmic protein; oxidizes fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]; enoyl-CoA hydratase, EchA3 complement(4793790..4794551) Mycobacterium ulcerans Agy99 4550279 YP_907804.1 CDS MUL_4320 NC_008611.1 4794652 4795122 D membrane protein; 19 kDa lipoprotein antigen LpqH-like protein 4794652..4795122 Mycobacterium ulcerans Agy99 4550280 YP_907805.1 CDS MUL_4324 NC_008611.1 4798616 4799947 D cytoplasmic protein; hypothetical protein 4798616..4799947 Mycobacterium ulcerans Agy99 4550284 YP_907806.1 CDS MUL_4325 NC_008611.1 4799944 4801098 D cytoplasmic protein; hypothetical protein 4799944..4801098 Mycobacterium ulcerans Agy99 4550285 YP_907807.1 CDS cut5 NC_008611.1 4802348 4803043 D secreted protein; hydrolysis of cutin (a polyester that forms the structure of plant cuticle); cutinase 4802348..4803043 Mycobacterium ulcerans Agy99 4550287 YP_907808.1 CDS MUL_4328 NC_008611.1 4803084 4804076 D membrane protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 4803084..4804076 Mycobacterium ulcerans Agy99 4550288 YP_907809.1 CDS MUL_4330 NC_008611.1 4804272 4804655 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(4804272..4804655) Mycobacterium ulcerans Agy99 4550289 YP_907810.1 CDS MUL_4332 NC_008611.1 4808057 4808368 D cytoplasmic protein; hypothetical protein 4808057..4808368 Mycobacterium ulcerans Agy99 4550291 YP_907811.1 CDS MUL_4333 NC_008611.1 4808365 4808943 D cytoplasmic protein; hypothetical protein 4808365..4808943 Mycobacterium ulcerans Agy99 4550292 YP_907812.1 CDS MUL_4336 NC_008611.1 4812375 4812689 D PE5_1; Detected in the membrane fraction by proteomics.; membrane protein; PE family protein 4812375..4812689 Mycobacterium ulcerans Agy99 4550294 YP_907813.1 CDS MUL_4338 NC_008611.1 4813978 4814388 R cytoplasmic protein; hypothetical protein complement(4813978..4814388) Mycobacterium ulcerans Agy99 4550296 YP_907814.1 CDS MUL_4339 NC_008611.1 4814427 4815467 R cytoplasmic protein; hypothetical protein complement(4814427..4815467) Mycobacterium ulcerans Agy99 4550297 YP_907815.1 CDS ligC NC_008611.1 4815505 4816581 D catalyzes the ATP-dependent formation of a phosphodiester at the site of a single-strand break in duplex DNA; in mycobacteria LigC has weak intrinsic nick joining activities and is not essential for growth; ATP-dependent DNA ligase 4815505..4816581 Mycobacterium ulcerans Agy99 4550500 YP_907816.1 CDS MUL_4341 NC_008611.1 4816680 4817222 D cytoplasmic protein; hypothetical protein 4816680..4817222 Mycobacterium ulcerans Agy99 4550501 YP_907817.1 CDS MUL_4343 NC_008611.1 4818580 4819674 D membrane protein; hypothetical protein 4818580..4819674 Mycobacterium ulcerans Agy99 4550503 YP_907818.1 CDS MUL_4344 NC_008611.1 4819615 4820043 R cytoplasmic protein; hypothetical protein complement(4819615..4820043) Mycobacterium ulcerans Agy99 4550504 YP_907819.1 CDS acrA1_1 NC_008611.1 4821484 4822563 R cytoplasmic protein; function unknown, involved in cellular metabolism; multi-functional enzyme with acyl-CoA-reductase activity AcrA1 complement(4821484..4822563) Mycobacterium ulcerans Agy99 4550506 YP_907820.1 CDS MUL_4347 NC_008611.1 4822749 4823555 D cytoplasmic protein; hypothetical protein 4822749..4823555 Mycobacterium ulcerans Agy99 4550507 YP_907821.1 CDS MUL_4348 NC_008611.1 4823718 4824110 D membrane protein; hypothetical protein 4823718..4824110 Mycobacterium ulcerans Agy99 4550508 YP_907822.1 CDS fadD15_1 NC_008611.1 4824127 4825929 R cytoplasmic protein; function unknown, but involved in lipid degradation.; long-chain-fatty-acid-CoA ligase FadD15 complement(4824127..4825929) Mycobacterium ulcerans Agy99 4550509 YP_907823.1 CDS MUL_4350 NC_008611.1 4826189 4827208 D cytoplasmic protein; involved in transcriptional mechanism.; AraC/XylS family transcriptional regulator 4826189..4827208 Mycobacterium ulcerans Agy99 4550510 YP_907824.1 CDS MUL_4351 NC_008611.1 4827300 4828649 R membrane protein; hypothetical protein complement(4827300..4828649) Mycobacterium ulcerans Agy99 4550511 YP_907825.1 CDS MUL_4352 NC_008611.1 4828702 4830201 R Detected in the secreted fraction by proteomics.; secreted protein; function unknown role in oxidoreduction; monooxygenase-like flavoprotein complement(4828702..4830201) Mycobacterium ulcerans Agy99 4550512 YP_907826.1 CDS MUL_4353 NC_008611.1 4830507 4831592 D cytoplasmic protein; hypothetical protein 4830507..4831592 Mycobacterium ulcerans Agy99 4550513 YP_907827.1 CDS MUL_4354 NC_008611.1 4831589 4832404 D cytoplasmic protein; short chain dehydrogenase 4831589..4832404 Mycobacterium ulcerans Agy99 4550514 YP_907828.1 CDS serA4 NC_008611.1 4832399 4833433 R cytoplasmic protein; involved at the first committed step in the 'phosphorylated' pathway of L-serine biosynthesis [catalytic activity: 3-phosphoglycerate + NAD(+) = 3- phosphohydroxypyruvate + NADH]; D-3-phosphoglycerate dehydrogenase complement(4832399..4833433) Mycobacterium ulcerans Agy99 4550515 YP_907829.1 CDS MUL_4356 NC_008611.1 4833556 4837482 D membrane protein; involved in lipid metabolism.; non-ribosomal peptide synthetase 4833556..4837482 Mycobacterium ulcerans Agy99 4550516 YP_907830.1 CDS pepN_1 NC_008611.1 4837479 4838804 D cytoplasmic protein; aminopeptidase with broad substrate specificity to several peptides (could preferentially cleave leucine, arginine and lysine in peptide-bond-containing substrates); aminopeptidase N PepN 4837479..4838804 Mycobacterium ulcerans Agy99 4550517 YP_907831.1 CDS MUL_4358 NC_008611.1 4838817 4839203 R cytoplasmic protein; hypothetical protein complement(4838817..4839203) Mycobacterium ulcerans Agy99 4550518 YP_907832.1 CDS MUL_4359 NC_008611.1 4839268 4839603 R PE34; membrane protein; PE family protein complement(4839268..4839603) Mycobacterium ulcerans Agy99 4550519 YP_907833.1 CDS MUL_4360 NC_008611.1 4840149 4840532 D cytoplasmic protein; hypothetical protein 4840149..4840532 Mycobacterium ulcerans Agy99 4550399 YP_907834.1 CDS MUL_4361 NC_008611.1 4840662 4841399 R cytoplasmic protein; function unknown, supposed involvement in cellular metabolism.; short-chain type dehydrogenase/reductase complement(4840662..4841399) Mycobacterium ulcerans Agy99 4550400 YP_907835.1 CDS MUL_4362 NC_008611.1 4841408 4842664 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; hydrolase complement(4841408..4842664) Mycobacterium ulcerans Agy99 4550401 YP_907836.1 CDS MUL_4363 NC_008611.1 4842661 4843932 R cytoplasmic protein; hypothetical protein complement(4842661..4843932) Mycobacterium ulcerans Agy99 4550402 YP_907837.1 CDS MUL_4364 NC_008611.1 4844061 4844546 D membrane protein; hypothetical protein 4844061..4844546 Mycobacterium ulcerans Agy99 4550403 YP_907838.1 CDS MUL_4365 NC_008611.1 4844618 4845001 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; unknwon; hypothetical protein 4844618..4845001 Mycobacterium ulcerans Agy99 4550404 YP_907839.1 CDS MUL_4366 NC_008611.1 4845060 4845419 D cytoplasmic protein; hypothetical protein 4845060..4845419 Mycobacterium ulcerans Agy99 4550405 YP_907840.1 CDS MUL_4367 NC_008611.1 4845511 4848744 R membrane protein; PE-PGRS family protein complement(4845511..4848744) Mycobacterium ulcerans Agy99 4550406 YP_907841.1 CDS MUL_4368 NC_008611.1 4848805 4850139 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(4848805..4850139) Mycobacterium ulcerans Agy99 4550407 YP_907842.1 CDS MUL_4370 NC_008611.1 4850766 4851224 R cytoplasmic protein; function unknown, probably involved in deamination of specific substrate.; cytidine/deoxycytidylate deaminase complement(4850766..4851224) Mycobacterium ulcerans Agy99 4550409 YP_907843.1 CDS MUL_4371 NC_008611.1 4851221 4851742 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(4851221..4851742) Mycobacterium ulcerans Agy99 4550410 YP_907844.1 CDS tyrA NC_008611.1 4851874 4852818 D catalyzes the formation of 4-hydroxyphenylpyruvate from prephenate; prephenate dehydrogenase 4851874..4852818 Mycobacterium ulcerans Agy99 4550411 YP_907845.1 CDS MUL_4373 NC_008611.1 4852781 4853380 R cytoplasmic protein; hypothetical protein complement(4852781..4853380) Mycobacterium ulcerans Agy99 4550412 YP_907846.1 CDS proZ NC_008611.1 4853385 4854131 R membrane protein; thought to be involved in active transport of osmoprotectant (glycine betaine/carnitine/choline/L- proline) across the membrane (import) responsible for the translocation of the substrate across the membrane.; osmoprotectant (glycine betaine/carnitine/choline/L-proline) transport integral membrane protein ABC transporter ProZ complement(4853385..4854131) Mycobacterium ulcerans Agy99 4550413 YP_907847.1 CDS proW NC_008611.1 4854128 4854808 R membrane protein; thought to be involved in active transport of osmoprotectant (glycine betaine/carnitine/choline/L- proline) across the membrane (import) responsible for the translocation of the substrate across the membrane.; osmoprotectant (glycine betaine/carnitine/choline/L-proline) transport integral membrane protein ABC transporter ProW complement(4854128..4854808) Mycobacterium ulcerans Agy99 4550383 YP_907848.1 CDS proX NC_008611.1 4855925 4856872 R secreted protein; thought to be involved in active transport of osmoprotectant (glycine betaine/carnitine/choline/L- proline) across the membrane (import); osmoprotectant (glycine betaine/carnitine/choline/L-proline) binding lipoprotein ProX complement(4855925..4856872) Mycobacterium ulcerans Agy99 4550385 YP_907849.1 CDS MUL_4378 NC_008611.1 4856987 4857364 D membrane protein; hypothetical protein 4856987..4857364 Mycobacterium ulcerans Agy99 4550386 YP_907850.1 CDS MUL_4380 NC_008611.1 4858571 4859179 R cytoplasmic protein; hypothetical protein complement(4858571..4859179) Mycobacterium ulcerans Agy99 4550388 YP_907851.1 CDS MUL_4381 NC_008611.1 4859588 4862377 D secreted protein; PE-PGRS family protein 4859588..4862377 Mycobacterium ulcerans Agy99 4550389 YP_907852.1 CDS MUL_4383 NC_008611.1 4863715 4864767 D cytoplasmic protein; hypothetical protein 4863715..4864767 Mycobacterium ulcerans Agy99 4550391 YP_907853.1 CDS MUL_4384 NC_008611.1 4864769 4865533 D cytoplasmic protein; function unknown, possibly involved in cellular metabolism.; short-chain type dehydrogenase/reductase 4864769..4865533 Mycobacterium ulcerans Agy99 4550392 YP_907854.1 CDS fadE1_1 NC_008611.1 4865551 4866858 D cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE1 4865551..4866858 Mycobacterium ulcerans Agy99 4550393 YP_907855.1 CDS MUL_4386 NC_008611.1 4866855 4867457 D cytoplasmic protein; hypothetical protein 4866855..4867457 Mycobacterium ulcerans Agy99 4550394 YP_907856.1 CDS MUL_4387 NC_008611.1 4867458 4868426 D membrane protein; possibly binds NADP and acts through a one-electron transfer process. quinones are supposed to be the best substrates. may act in the detoxification of xenobiotics [catalytic activity: NADPH + quinone = NADP+ + semiquinone]; quinone oxidoreductase 4867458..4868426 Mycobacterium ulcerans Agy99 4550395 YP_907857.1 CDS MUL_4388 NC_008611.1 4868466 4870313 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; hydrolase complement(4868466..4870313) Mycobacterium ulcerans Agy99 4550396 YP_907858.1 CDS lpqH NC_008611.1 4870493 4870978 D secreted protein; 19 kDa lipoprotein antigen precursor LpqH 4870493..4870978 Mycobacterium ulcerans Agy99 4550397 YP_907859.1 CDS tcrX NC_008611.1 4872737 4873441 R cytoplasmic protein; sensor part of a two component regulatory system.; two component transcriptional regulatory protein TcrX complement(4872737..4873441) Mycobacterium ulcerans Agy99 4550557 YP_907860.1 CDS MUL_4392 NC_008611.1 4873519 4874853 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 4873519..4874853 Mycobacterium ulcerans Agy99 4550558 YP_907861.1 CDS MUL_4394 NC_008611.1 4876384 4876539 R membrane protein; hypothetical protein complement(4876384..4876539) Mycobacterium ulcerans Agy99 4550560 YP_907862.1 CDS MUL_4397 NC_008611.1 4877141 4878475 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(4877141..4878475) Mycobacterium ulcerans Agy99 4550563 YP_907863.1 CDS MUL_4398 NC_008611.1 4878906 4879952 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 4878906..4879952 Mycobacterium ulcerans Agy99 4550564 YP_907864.1 CDS MUL_4399 NC_008611.1 4880207 4881649 R membrane protein; PE-PGRS family protein complement(4880207..4881649) Mycobacterium ulcerans Agy99 4550565 YP_907865.1 CDS MUL_4400 NC_008611.1 4881794 4883275 R membrane protein; PE-PGRS family protein complement(4881794..4883275) Mycobacterium ulcerans Agy99 4550566 YP_907866.1 CDS MUL_4401 NC_008611.1 4883410 4884456 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(4883410..4884456) Mycobacterium ulcerans Agy99 4550567 YP_907867.1 CDS MUL_4402 NC_008611.1 4884825 4885769 R cytoplasmic protein; function unknown, may play a role in secondary metabolites biosynthesis, transport, and catabolism.; O-methyltransferase complement(4884825..4885769) Mycobacterium ulcerans Agy99 4550568 YP_907868.1 CDS MUL_4403 NC_008611.1 4885894 4886241 D cytoplasmic protein; hypothetical protein 4885894..4886241 Mycobacterium ulcerans Agy99 4550569 YP_907869.1 CDS MUL_4404 NC_008611.1 4886283 4887275 D cytoplasmic protein; hypothetical protein 4886283..4887275 Mycobacterium ulcerans Agy99 4549887 YP_907870.1 CDS hisC2 NC_008611.1 4887661 4888746 D cytoplasmic protein; involved in histidine biosynthetic pathway (at the eighth step) [catalytic activity: L-histidinol-phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + glutamate]; putative aminotransferase 4887661..4888746 Mycobacterium ulcerans Agy99 4549890 YP_907871.1 CDS MUL_4408 NC_008611.1 4888743 4889180 D cytoplasmic protein; hypothetical protein 4888743..4889180 Mycobacterium ulcerans Agy99 4549891 YP_907872.1 CDS MUL_4409 NC_008611.1 4889228 4889812 R cytoplasmic protein; hypothetical protein complement(4889228..4889812) Mycobacterium ulcerans Agy99 4549892 YP_907873.1 CDS MUL_4410 NC_008611.1 4889836 4891941 D Point mutation has removed stop codon and translationally coupled EchA21 with LipE; Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); cytoplasmic protein; function unknown, lipolytic enzyme probably involved in lipid metabolism.; fusion of enoyl-CoA hydratase, EchA21 and lipase, LipE 4889836..4891941 Mycobacterium ulcerans Agy99 4549893 YP_907874.1 CDS MUL_4412 NC_008611.1 4892343 4893347 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(4892343..4893347) Mycobacterium ulcerans Agy99 4549895 YP_907875.1 CDS MUL_4413 NC_008611.1 4895350 4895664 D cytoplasmic protein; hypothetical protein 4895350..4895664 Mycobacterium ulcerans Agy99 4549897 YP_907876.1 CDS MUL_4415 NC_008611.1 4896524 4897570 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(4896524..4897570) Mycobacterium ulcerans Agy99 4549899 YP_907877.1 CDS pgi NC_008611.1 4898115 4899779 D functions in sugar metabolism in glycolysis and the Embden-Meyerhof pathways (EMP) and in gluconeogenesis; catalyzes reversible isomerization of glucose-6-phosphate to fructose-6-phosphate; member of PGI family; glucose-6-phosphate isomerase 4898115..4899779 Mycobacterium ulcerans Agy99 4549900 YP_907878.1 CDS MUL_4417 NC_008611.1 4899879 4900628 R Also detected in the membrane and the cytoplasmic fractions by proteomics; secreted protein; function unknown, possibly involved in cellular metabolism.; short chain dehydrogenase complement(4899879..4900628) Mycobacterium ulcerans Agy99 4549901 YP_907879.1 CDS MUL_4418 NC_008611.1 4900716 4901579 R Detected in the cytoplamic fraction by LCMSMS; cytoplasmic protein; this enzyme may play a significant role in processes leading to recovery from mutagenesis and/or cell death by alkylating agents [catalytic activity: hydrolysis of DNA containing ring-opened N7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N- methyl)formamidopyrimide]; formamidopyrimidine-DNA glycosylase complement(4900716..4901579) Mycobacterium ulcerans Agy99 4550209 YP_907880.1 CDS MUL_4419 NC_008611.1 4901627 4902679 R membrane protein; PE-PGRS family protein complement(4901627..4902679) Mycobacterium ulcerans Agy99 4550210 YP_907881.1 CDS MUL_4422 NC_008611.1 4906235 4906978 R catalyzes the formation of precorrin 6x from precorrin 5; precorrin 6A synthase complement(4906235..4906978) Mycobacterium ulcerans Agy99 4550213 YP_907882.1 CDS MUL_4424 NC_008611.1 4907116 4908039 D membrane protein; hypothetical protein 4907116..4908039 Mycobacterium ulcerans Agy99 4550214 YP_907883.1 CDS MUL_4425 NC_008611.1 4908129 4909340 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; monooxygenase 4908129..4909340 Mycobacterium ulcerans Agy99 4550215 YP_907884.1 CDS MUL_4427 NC_008611.1 4909897 4910661 D membrane protein; hypothetical protein 4909897..4910661 Mycobacterium ulcerans Agy99 4550216 YP_907885.1 CDS echA12_2 NC_008611.1 4911680 4912471 R cytoplasmic protein; oxidizes fatty acids using specific components (by similarity) [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]; enoyl-CoA hydratase, EchA12_2 complement(4911680..4912471) Mycobacterium ulcerans Agy99 4550218 YP_907886.1 CDS MUL_4430 NC_008611.1 4912594 4913517 D cytoplasmic protein; hypothetical protein 4912594..4913517 Mycobacterium ulcerans Agy99 4550219 YP_907887.1 CDS MUL_4431 NC_008611.1 4913637 4915358 D membrane protein; PE-PGRS family protein 4913637..4915358 Mycobacterium ulcerans Agy99 4550220 YP_907888.1 CDS MUL_4434 NC_008611.1 4917308 4919620 R catalyzes the ATP-dependent formation of a phosphodiester at the site of a single-strand break in duplex DNA and has been shown to have polymerase activity; ATP-dependent DNA ligase complement(4917308..4919620) Mycobacterium ulcerans Agy99 4550222 YP_907889.1 CDS pfkB NC_008611.1 4919643 4920554 D membrane protein; ATP + D-fructose = ADP + D-fructose 6-phosphate; fructokinase, PfkB 4919643..4920554 Mycobacterium ulcerans Agy99 4550223 YP_907890.1 CDS MUL_4436 NC_008611.1 4920726 4921601 D cytoplasmic protein; hypothetical protein 4920726..4921601 Mycobacterium ulcerans Agy99 4550224 YP_907891.1 CDS pstS2 NC_008611.1 4921675 4922778 D Detected in the membrane fraction by proteomics.; membrane protein; involved in active transport of inorganic phosphate across the membrane (import) this is one of the proteins required for binding-protein-mediated phosphate transport.; periplasmic phosphate-binding lipoprotein PstS2 4921675..4922778 Mycobacterium ulcerans Agy99 4550225 YP_907892.1 CDS pstA1 NC_008611.1 4925201 4926103 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. this is one of the proteins required for binding-protein- mediated phosphate transport.; phosphate-transport integral membrane ABC transporter PstA1 complement(4925201..4926103) Mycobacterium ulcerans Agy99 4550227 YP_907893.1 CDS pstC2 NC_008611.1 4926100 4927074 R membrane protein; involved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. this is one of the proteins required for binding-protein- mediated phosphate transport.; phosphate-transport integral membrane ABC transporter PstC2 complement(4926100..4927074) Mycobacterium ulcerans Agy99 4550228 YP_907894.1 CDS phoS2 NC_008611.1 4927087 4928199 R Detected in the membrane and the secreted protein fractions by proteomics.; secreted protein; involved in active transport of inorganic phosphate across the membrane (import) this is one of the proteins required for binding-protein-mediated phosphate transport.; periplasmic phosphate-binding lipoprotein PhoS2 complement(4927087..4928199) Mycobacterium ulcerans Agy99 4550229 YP_907895.1 CDS MUL_4442 NC_008611.1 4928533 4929324 D cytoplasmic protein; function unknown, possibly involved in cellular metabolism.; short chain dehydrogenase 4928533..4929324 Mycobacterium ulcerans Agy99 4550230 YP_907896.1 CDS MUL_4444 NC_008611.1 4930549 4931283 D hypothetical protein 4930549..4931283 Mycobacterium ulcerans Agy99 4550232 YP_907897.1 CDS mntH NC_008611.1 4931363 4932589 D membrane protein; H(+)-stimulated, highly selective, divalent cation uptake system. responsible for the translocation of the divalent metal across the membrane.; manganese transport protein MntH 4931363..4932589 Mycobacterium ulcerans Agy99 4550233 YP_907898.1 CDS MUL_4446 NC_008611.1 4932755 4933084 D secreted protein; hypothetical protein 4932755..4933084 Mycobacterium ulcerans Agy99 4550234 YP_907899.1 CDS MUL_4447 NC_008611.1 4933201 4934238 D CDS appears to have been formed by a DNA deletion that fused the N-term of a conserved hypothetical protein with the C-term of a putative oxidoreductase (MON4219); cytoplasmic protein; hypothetical protein 4933201..4934238 Mycobacterium ulcerans Agy99 4550235 YP_907900.1 CDS MUL_4449 NC_008611.1 4934427 4934579 R cytoplasmic protein; hypothetical protein complement(4934427..4934579) Mycobacterium ulcerans Agy99 4550237 YP_907901.1 CDS MUL_4450 NC_008611.1 4934757 4935671 R cytoplasmic protein; hypothetical protein complement(4934757..4935671) Mycobacterium ulcerans Agy99 4550238 YP_907902.1 CDS MUL_4451 NC_008611.1 4935896 4936195 D cytoplasmic protein; hypothetical protein 4935896..4936195 Mycobacterium ulcerans Agy99 4550239 YP_907903.1 CDS MUL_4453 NC_008611.1 4938729 4939241 R cytoplasmic protein; hypothetical protein complement(4938729..4939241) Mycobacterium ulcerans Agy99 4550241 YP_907904.1 CDS MUL_4454 NC_008611.1 4939315 4939896 D cytoplasmic protein; transposition of an insertion sequence. possibly IS200 family.; transposase 4939315..4939896 Mycobacterium ulcerans Agy99 4550242 YP_907905.1 CDS MUL_4455 NC_008611.1 4939739 4940296 R membrane protein; hypothetical protein complement(4939739..4940296) Mycobacterium ulcerans Agy99 4550192 YP_907906.1 CDS MUL_4456 NC_008611.1 4940510 4941820 R membrane protein; PPE family protein complement(4940510..4941820) Mycobacterium ulcerans Agy99 4550193 YP_907907.1 CDS MUL_4458 NC_008611.1 4942872 4943651 D membrane protein; hypothetical protein 4942872..4943651 Mycobacterium ulcerans Agy99 4550194 YP_907908.1 CDS MUL_4459 NC_008611.1 4943924 4944157 D membrane protein; hypothetical protein 4943924..4944157 Mycobacterium ulcerans Agy99 4550195 YP_907909.1 CDS MUL_4460 NC_008611.1 4944423 4944887 D cytoplasmic protein; hypothetical protein 4944423..4944887 Mycobacterium ulcerans Agy99 4550196 YP_907910.1 CDS MUL_4461 NC_008611.1 4945577 4946380 D membrane protein; hypothetical protein 4945577..4946380 Mycobacterium ulcerans Agy99 4550197 YP_907911.1 CDS MUL_4462 NC_008611.1 4946694 4946993 D membrane protein; PE family protein 4946694..4946993 Mycobacterium ulcerans Agy99 4550198 YP_907912.1 CDS MUL_4463 NC_008611.1 4946999 4948270 D membrane protein; function unknown. possibly a protective antigen involved with the early control of infection.; PPE family protein 4946999..4948270 Mycobacterium ulcerans Agy99 4550199 YP_907913.1 CDS MUL_4465 NC_008611.1 4949701 4951035 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(4949701..4951035) Mycobacterium ulcerans Agy99 4550201 YP_907914.1 CDS MUL_4469 NC_008611.1 4955972 4956496 R cytoplasmic protein; function unknown. seems to be expressed during macrophage infection.; hypothetical protein complement(4955972..4956496) Mycobacterium ulcerans Agy99 4550204 YP_907915.1 CDS MUL_4470 NC_008611.1 4956797 4957978 R cytoplasmic protein; possibly involved in camp synthesis [catalytic activity: ATP = 3',5'-cyclic AMP + diphosphate]; adenylate cyclase complement(4956797..4957978) Mycobacterium ulcerans Agy99 4550205 YP_907916.1 CDS pknF NC_008611.1 4958078 4959565 D cytoplasmic protein; involved in signal transduction (via phosphorylation) thought to be involved in membrane transport. phosphorylates the peptide substrate myelin basic protein (MBP) at serine and threonine residues [catalytic activity: ATP + a protein = ADP + a phosphoprotein]; anchored-membrane serine/threonine-protein kinase PknF 4958078..4959565 Mycobacterium ulcerans Agy99 4550206 YP_907917.1 CDS MUL_4473 NC_008611.1 4959831 4960877 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(4959831..4960877) Mycobacterium ulcerans Agy99 4550208 YP_907918.1 CDS MUL_4474 NC_008611.1 4961281 4962615 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(4961281..4962615) Mycobacterium ulcerans Agy99 4549793 YP_907919.1 CDS MUL_4476 NC_008611.1 4963728 4964774 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 4963728..4964774 Mycobacterium ulcerans Agy99 4549796 YP_907920.1 CDS amiB2 NC_008611.1 4965103 4966491 R catalyzes the hydrolysis of a monocarboxylic acid amid to form a monocarboxylate and ammonia; amidase complement(4965103..4966491) Mycobacterium ulcerans Agy99 4549797 YP_907921.1 CDS MUL_4479 NC_008611.1 4966594 4967022 D cytoplasmic protein; Hit-like protein 4966594..4967022 Mycobacterium ulcerans Agy99 4549798 YP_907922.1 CDS MUL_4480 NC_008611.1 4967033 4967485 D hypothetical protein 4967033..4967485 Mycobacterium ulcerans Agy99 4549799 YP_907923.1 CDS MUL_4481 NC_008611.1 4967494 4968546 R cytoplasmic protein; monooxygenase complement(4967494..4968546) Mycobacterium ulcerans Agy99 4549800 YP_907924.1 CDS MUL_4482 NC_008611.1 4968584 4969720 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; hypothetical protein complement(4968584..4969720) Mycobacterium ulcerans Agy99 4549801 YP_907925.1 CDS MUL_4483 NC_008611.1 4969724 4970551 R cytoplasmic protein; hypothetical protein complement(4969724..4970551) Mycobacterium ulcerans Agy99 4549802 YP_907926.1 CDS MUL_4485 NC_008611.1 4971932 4973299 D cytoplasmic protein; function unknown, probably involved in cellular metabolism; oxidoreductase 4971932..4973299 Mycobacterium ulcerans Agy99 4549804 YP_907927.1 CDS MUL_4487 NC_008611.1 4974745 4975326 D cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein 4974745..4975326 Mycobacterium ulcerans Agy99 4549806 YP_907928.1 CDS MUL_4489 NC_008611.1 4975841 4977163 D membrane protein; PE-PGRS family protein 4975841..4977163 Mycobacterium ulcerans Agy99 4549807 YP_907929.1 CDS MUL_4490 NC_008611.1 4977316 4977537 R cytoplasmic protein; hypothetical protein complement(4977316..4977537) Mycobacterium ulcerans Agy99 4549808 YP_907930.1 CDS MUL_4491 NC_008611.1 4977801 4978550 D membrane protein; PPE family protein 4977801..4978550 Mycobacterium ulcerans Agy99 4549809 YP_907931.1 CDS MUL_4492 NC_008611.1 4978641 4979783 R membrane protein; catalyzes the acylation of the mycaminose sugar during midecamycin biosynthesis; integral membrane acyltransferase complement(4978641..4979783) Mycobacterium ulcerans Agy99 4549810 YP_907932.1 CDS lprE NC_008611.1 4981662 4982258 D membrane protein; lipoprotein LprE 4981662..4982258 Mycobacterium ulcerans Agy99 4549921 YP_907933.1 CDS MUL_4495 NC_008611.1 4982341 4985748 D cytoplasmic protein; hypothetical protein 4982341..4985748 Mycobacterium ulcerans Agy99 4549922 YP_907934.1 CDS MUL_4497 NC_008611.1 4986928 4987245 R cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 complement(4986928..4987245) Mycobacterium ulcerans Agy99 4549924 YP_907935.1 CDS MUL_4498 NC_008611.1 4987270 4987974 R cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 complement(4987270..4987974) Mycobacterium ulcerans Agy99 4549925 YP_907936.1 CDS MUL_4499 NC_008611.1 4989118 4989876 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 4989118..4989876 Mycobacterium ulcerans Agy99 4549926 YP_907937.1 CDS kgd NC_008611.1 4990076 4993792 D kgd; produces succinic semialdehyde; part of alternative pathway from alpha-ketoglutarate to succinate; essential for normal growth; alpha-ketoglutarate decarboxylase 4990076..4993792 Mycobacterium ulcerans Agy99 4549927 YP_907938.1 CDS MUL_4501 NC_008611.1 4993949 4994782 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (LC-MS/MS); cytoplasmic protein; function unknown, supposed involved in cellular metabolism.; short-chain type dehydrogenase/reductase 4993949..4994782 Mycobacterium ulcerans Agy99 4549928 YP_907939.1 CDS lpqZ NC_008611.1 4994819 4995670 R secreted protein; lipoprotein LpqZ complement(4994819..4995670) Mycobacterium ulcerans Agy99 4549929 YP_907940.1 CDS mez_1 NC_008611.1 4995682 4996896 R membrane protein; catalizes the oxidative decarboxylation of malate into pyruvate, important for a wide range of metabolic pathways [catalytic activity: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH]; [NAD] dependent malate oxidoreductase Mez complement(4995682..4996896) Mycobacterium ulcerans Agy99 4549930 YP_907941.1 CDS mdh NC_008611.1 4997055 4998044 R catalyzes the oxidation of malate to oxaloacetate; malate dehydrogenase complement(4997055..4998044) Mycobacterium ulcerans Agy99 4549931 YP_907942.1 CDS MUL_4506 NC_008611.1 4998758 4999804 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(4998758..4999804) Mycobacterium ulcerans Agy99 4549933 YP_907943.1 CDS fgd2 NC_008611.1 5000709 5001854 R cytoplasmic protein; catalyzes oxidation of glucose-6-phosphate to 6- phosphogluconolactone using coenzyme F420 (an *-hydroxy-5- deazaflavin derivative) as the electron acceptor.; F420-dependent glucose-6-phosphate dehydrogenase Fgd2 complement(5000709..5001854) Mycobacterium ulcerans Agy99 4549934 YP_907944.1 CDS gabP_1 NC_008611.1 5002096 5003541 R membrane protein; involved in 4-aminobutyrate (GabA) degradation pathway. transporter for GabA. responsible for the translocation of the substrate across the membrane.; GabA permease GabP complement(5002096..5003541) Mycobacterium ulcerans Agy99 4549935 YP_907945.1 CDS MUL_4509 NC_008611.1 5003822 5004394 R membrane protein; hypothetical protein complement(5003822..5004394) Mycobacterium ulcerans Agy99 4549936 YP_907946.1 CDS sugB NC_008611.1 5005603 5006346 R membrane protein; involved in active transport of sugar across the membrane (import) responsible for the translocation of the substrate across the membrane.; sugar-transport integral membrane protein ABC transporter SugB complement(5005603..5006346) Mycobacterium ulcerans Agy99 4549903 YP_907947.1 CDS sugA NC_008611.1 5006343 5007380 R secreted protein; involved in active transport of sugar across the membrane (import) responsible for the translocation of the substrate across the membrane.; sugar-transport integral membrane protein ABC transporter SugA complement(5006343..5007380) Mycobacterium ulcerans Agy99 4549904 YP_907948.1 CDS MUL_4514 NC_008611.1 5008259 5009665 D membrane protein 5008259..5009665 Mycobacterium ulcerans Agy99 4549906 YP_907949.1 CDS mrp NC_008611.1 5009717 5010877 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown: thought to be an ATP-binding protein.; Mrp-related protein Mrp 5009717..5010877 Mycobacterium ulcerans Agy99 4549907 YP_907950.1 CDS tatB NC_008611.1 5010893 5011285 R mediates the export of protein precursors bearing twin-arginine signal peptides; sec-independent translocase complement(5010893..5011285) Mycobacterium ulcerans Agy99 4549908 YP_907951.1 CDS htrA NC_008611.1 5011285 5012799 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; possibly hydrolyzes peptides and/or proteins (seems to cleave preferentially after serine residue); serine protease HtrA (DegP protein) complement(5011285..5012799) Mycobacterium ulcerans Agy99 4549909 YP_907952.1 CDS MUL_4518 NC_008611.1 5012971 5013375 R cytoplasmic protein; hypothetical protein complement(5012971..5013375) Mycobacterium ulcerans Agy99 4549910 YP_907953.1 CDS sigE NC_008611.1 5013521 5014288 R Member of the extracytoplasmic function sigma factors which are active under specific conditions; binds with the catalytic core of RNA polymerase to produce the holoenzyme and directs bacterial core RNA polymerase to specific promoter elements to initiate transcription; in M. tuberculosis this protein is involved in heat shock, oxidative stress and virulence; RNA polymerase sigma factor SigE complement(5013521..5014288) Mycobacterium ulcerans Agy99 4549911 YP_907954.1 CDS MUL_4520 NC_008611.1 5014511 5015185 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown, involved in cellular metabolism; methyltransferase 5014511..5015185 Mycobacterium ulcerans Agy99 4549912 YP_907955.1 CDS MUL_4523 NC_008611.1 5018053 5018727 D membrane protein; integral membrane protein 5018053..5018727 Mycobacterium ulcerans Agy99 4549915 YP_907956.1 CDS MUL_4524 NC_008611.1 5018940 5020202 D membrane protein; PE-PGRS family protein 5018940..5020202 Mycobacterium ulcerans Agy99 4549916 YP_907957.1 CDS glgC NC_008611.1 5021850 5023064 R catalyzes the formation of ADP-glucose and diphosphate from ATP and alpha-D-glucose 1-phosphate; glucose-1-phosphate adenylyltransferase complement(5021850..5023064) Mycobacterium ulcerans Agy99 4549918 YP_907958.1 CDS MUL_4529 NC_008611.1 5026333 5027379 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(5026333..5027379) Mycobacterium ulcerans Agy99 4549812 YP_907959.1 CDS MUL_4531 NC_008611.1 5031585 5032919 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(5031585..5032919) Mycobacterium ulcerans Agy99 4549814 YP_907960.1 CDS MUL_4533 NC_008611.1 5033771 5034337 R Detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; hypothetical protein complement(5033771..5034337) Mycobacterium ulcerans Agy99 4549816 YP_907961.1 CDS MUL_4534 NC_008611.1 5034334 5035758 R cytoplasmic protein; possibly involved in transcriptional mechanism.; transcriptional regulatory protein complement(5034334..5035758) Mycobacterium ulcerans Agy99 4549817 YP_907962.1 CDS MUL_4535 NC_008611.1 5035933 5036727 D cytoplasmic protein; hypothetical protein 5035933..5036727 Mycobacterium ulcerans Agy99 4549818 YP_907963.1 CDS icl NC_008611.1 5037035 5038321 D Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); cytoplasmic protein; involved in glyoxylate bypass (at the first step), an alternative to the tricarboxylic acid cycle (in bacteria, plants, and fungi) [catalytic activity: isocitrate = succinate + glyoxylate] involved in the persistence in the host.; isocitrate lyase Icl 5037035..5038321 Mycobacterium ulcerans Agy99 4549819 YP_907964.1 CDS fadB2 NC_008611.1 5038485 5039351 D converts (S)-3-hydroxybutanoyl-CoA to 3-acetoacetyl-CoA; 3-hydroxybutyryl-CoA dehydrogenase 5038485..5039351 Mycobacterium ulcerans Agy99 4549820 YP_907965.1 CDS umaA NC_008611.1 5039487 5040347 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (LC-MS/MS); cytoplasmic protein; involved in mycolic acid modification or synthesis.; mycolic acid synthase UmaA 5039487..5040347 Mycobacterium ulcerans Agy99 4549821 YP_907966.1 CDS pcaA NC_008611.1 5040579 5041442 R cytoplasmic protein; involved in the mycolic acid modification or synthesis; essential for the cyclopropanation function. required for cording and mycolic acid cyclopropane ring synthesis in the cell wall.; mycolic acid synthase PcaA complement(5040579..5041442) Mycobacterium ulcerans Agy99 4549822 YP_907967.1 CDS MUL_4540 NC_008611.1 5041599 5042282 R cytoplasmic protein; possibly involved in transcriptional mechanism.; TetR family transcriptional regulator complement(5041599..5042282) Mycobacterium ulcerans Agy99 4549823 YP_907968.1 CDS MUL_4541 NC_008611.1 5042417 5043778 D membrane protein; transmembrane protein 5042417..5043778 Mycobacterium ulcerans Agy99 4549824 YP_907969.1 CDS MUL_4542 NC_008611.1 5043864 5044280 D cytoplasmic protein; possibly involved in transcriptional mechanism.; transcriptional regulatory protein 5043864..5044280 Mycobacterium ulcerans Agy99 4549825 YP_907970.1 CDS hbhA NC_008611.1 5044620 5045240 D Also detected in the cytoplasmic fraction y proteomics; membrane protein; required for extrapulmonary dissemination. mediates adherence to epithelial cells by binding to sulfated glycoconjugates present at the surface of these cells; binds heparin, dextran sulfate, fucoidan and chondroitin sulfate. promotes hemagglutination of erythrocytes of certain host species. induces mycobacterial aggregation.; iron-regulated heparin binding hemagglutinin HbhA 5044620..5045240 Mycobacterium ulcerans Agy99 4549826 YP_907971.1 CDS MUL_4544 NC_008611.1 5045393 5045680 D membrane protein; transmembrane protein 5045393..5045680 Mycobacterium ulcerans Agy99 4549827 YP_907972.1 CDS MUL_4545 NC_008611.1 5045685 5046167 D secreted protein; hypothetical protein 5045685..5046167 Mycobacterium ulcerans Agy99 4549828 YP_907973.1 CDS deoC NC_008611.1 5046167 5046847 D catalyzes the formation of D-glyceraldehyde 3-phosphate and acetaldehyde from 2-deoxy-D-ribose-5-phosphate; deoxyribose-phosphate aldolase 5046167..5046847 Mycobacterium ulcerans Agy99 4554000 YP_907974.1 CDS MUL_4547 NC_008611.1 5046866 5047756 R Mutation has removed potential u/s start codon; Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(5046866..5047756) Mycobacterium ulcerans Agy99 4554001 YP_907975.1 CDS MUL_4548 NC_008611.1 5047909 5048751 R cytoplasmic protein; function unknown, hydrolytic enzyme probably involved in cellular metabolism.; amidohydrolase complement(5047909..5048751) Mycobacterium ulcerans Agy99 4554002 YP_907976.1 CDS MUL_4551 NC_008611.1 5050453 5050977 R cytoplasmic protein; hypothetical protein complement(5050453..5050977) Mycobacterium ulcerans Agy99 4554004 YP_907977.1 CDS murB NC_008611.1 5051004 5052104 D catalyzes the reduction of UDP-N-acetylglucosamine enolpyruvate to form UDP-N-acetylmuramate in peptidoglycan biosynthesis; UDP-N-acetylenolpyruvoylglucosamine reductase 5051004..5052104 Mycobacterium ulcerans Agy99 4554005 YP_907978.1 CDS lprQ NC_008611.1 5052114 5053529 D membrane protein; lipoprotein LprQ 5052114..5053529 Mycobacterium ulcerans Agy99 4554006 YP_907979.1 CDS MUL_4554 NC_008611.1 5053513 5054265 R cytoplasmic protein; function unknown, possibly involved in cellular metabolism.; short-chain type oxidoreductase complement(5053513..5054265) Mycobacterium ulcerans Agy99 4554007 YP_907980.1 CDS MUL_4555 NC_008611.1 5054508 5055743 D cytoplasmic protein; possibly involved in transcriptional mechanism.; transcriptional regulatory protein 5054508..5055743 Mycobacterium ulcerans Agy99 4554008 YP_907981.1 CDS MUL_4556 NC_008611.1 5055764 5057155 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS; cytoplasmic protein; thought to be involved in polyprenolmannose synthesis.; mannosyltransferase 5055764..5057155 Mycobacterium ulcerans Agy99 4554009 YP_907982.1 CDS MUL_4557 NC_008611.1 5057152 5057703 D cytoplasmic protein; hypothetical protein 5057152..5057703 Mycobacterium ulcerans Agy99 4554010 YP_907983.1 CDS gpmA NC_008611.1 5057717 5058472 D 2,3-bisphosphoglycerate-dependent; catalyzes the interconversion of 2-phosphoglycerate to 3-phosphoglycerate; phosphoglyceromutase 5057717..5058472 Mycobacterium ulcerans Agy99 4554011 YP_907984.1 CDS senX3 NC_008611.1 5058614 5059834 D membrane protein; sensor part of a two component regulatory system. probably forms part of a two-component regulatory system SenX3/RegX3; phosphorylates RegX3.; two component sensor histidine kinase SenX3 5058614..5059834 Mycobacterium ulcerans Agy99 4554012 YP_907985.1 CDS regX3 NC_008611.1 5059884 5060567 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; transcriptional regulatory protein part of the two component regulatory system RegX3/SenX3.; two component sensory transduction protein RegX3 5059884..5060567 Mycobacterium ulcerans Agy99 4554013 YP_907986.1 CDS MUL_4561 NC_008611.1 5060530 5062431 R membrane protein; function unknown, probably involved in cellular metabolism.; oxidoreductase GMC-type complement(5060530..5062431) Mycobacterium ulcerans Agy99 4554014 YP_907987.1 CDS MUL_4562 NC_008611.1 5062428 5062769 R membrane protein; hypothetical protein complement(5062428..5062769) Mycobacterium ulcerans Agy99 4554015 YP_907988.1 CDS MUL_4564 NC_008611.1 5063860 5064597 D cytoplasmic protein; involved in transcriptional mechanism.; GntR family transcriptional regulator 5063860..5064597 Mycobacterium ulcerans Agy99 4551451 YP_907989.1 CDS MUL_4565 NC_008611.1 5064610 5065404 R cytoplasmic protein; hypothetical protein complement(5064610..5065404) Mycobacterium ulcerans Agy99 4551452 YP_907990.1 CDS MUL_4566 NC_008611.1 5065544 5066578 D cytoplasmic protein; hypothetical protein 5065544..5066578 Mycobacterium ulcerans Agy99 4551453 YP_907991.1 CDS MUL_4567 NC_008611.1 5066575 5067639 D Also detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; transmembrane protein 5066575..5067639 Mycobacterium ulcerans Agy99 4551454 YP_907992.1 CDS MUL_4568 NC_008611.1 5067656 5068498 D cytoplasmic protein; hypothetical protein 5067656..5068498 Mycobacterium ulcerans Agy99 4551455 YP_907993.1 CDS MUL_4569 NC_008611.1 5068500 5069345 D cytoplasmic protein; hypothetical protein 5068500..5069345 Mycobacterium ulcerans Agy99 4551456 YP_907994.1 CDS proC NC_008611.1 5069370 5070254 D catalyzes the formation of L-proline from pyrroline-5-carboxylate; pyrroline-5-carboxylate reductase 5069370..5070254 Mycobacterium ulcerans Agy99 4551457 YP_907995.1 CDS MUL_4571 NC_008611.1 5070396 5070653 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (LC-MS/MS); cytoplasmic protein; hypothetical protein 5070396..5070653 Mycobacterium ulcerans Agy99 4551458 YP_907996.1 CDS MUL_4572 NC_008611.1 5070792 5070893 D cytoplasmic protein; hypothetical protein 5070792..5070893 Mycobacterium ulcerans Agy99 4551459 YP_907997.1 CDS galE2 NC_008611.1 5070972 5072105 D cytoplasmic protein; involved in galactose metabolism [catalytic activity: UDP-glucose = UDP-galactose]; UDP-glucose 4-epimerase GalE2 5070972..5072105 Mycobacterium ulcerans Agy99 4551460 YP_907998.1 CDS MUL_4574 NC_008611.1 5072087 5073178 D Detected in the secreted fraction by proteomics.; cytoplasmic protein; hypothetical protein 5072087..5073178 Mycobacterium ulcerans Agy99 4551461 YP_907999.1 CDS cmaA2 NC_008611.1 5073232 5074143 R cytoplasmic protein; essential for the cyclopropanation function. transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge. mycolic acids, which represent the major constituent of mycobacterial cell wall complex, act as substrates [catalytic activity: S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L- homocysteine + phospholipid cyclopropane fatty acid]; cyclopropane-fatty-acyl-phospholipid synthase 2 CmaA2 complement(5073232..5074143) Mycobacterium ulcerans Agy99 4551462 YP_908000.1 CDS MUL_4576 NC_008611.1 5074166 5074666 R cytoplasmic protein; hypothetical protein complement(5074166..5074666) Mycobacterium ulcerans Agy99 4551463 YP_908001.1 CDS serB1 NC_008611.1 5074711 5075622 R Detected in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; removes a phosphate from phosphoserine [catalytic activity: phosphoserine + H2O = serine + phosphate]; phosphoserine phosphatase SerB1 complement(5074711..5075622) Mycobacterium ulcerans Agy99 4551464 YP_908002.1 CDS coxS_1 NC_008611.1 5075810 5076412 D cytoplasmic protein; involved in cellular metabolism [catalytic activity: CO + H(2)O + acceptor = CO(2) + reduced acceptor]; carbon monoxyde dehydrogenase small chain CoxS 5075810..5076412 Mycobacterium ulcerans Agy99 4551465 YP_908003.1 CDS coxM_1 NC_008611.1 5076416 5077393 D cytoplasmic protein; function unknown, probably involved in cellular metabolism [catalytic activity: CO + H(2)O + acceptor = CO(2) + reduced acceptor]; carbon monoxyde dehydrogenase medium chain CoxM 5076416..5077393 Mycobacterium ulcerans Agy99 4551466 YP_908004.1 CDS coxL_1 NC_008611.1 5077390 5079567 D membrane protein; involved in cellular metabolism [catalytic activity: CO + H(2)O + acceptor = CO(2) + reduced acceptor]; carbon monoxyde dehydrogenase large chain CoxL 5077390..5079567 Mycobacterium ulcerans Agy99 4551467 YP_908005.1 CDS MUL_4581 NC_008611.1 5079607 5081247 R membrane protein; PE-PGRS family protein complement(5079607..5081247) Mycobacterium ulcerans Agy99 4551468 YP_908006.1 CDS MUL_4582 NC_008611.1 5081588 5082634 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 5081588..5082634 Mycobacterium ulcerans Agy99 4553067 YP_908007.1 CDS MUL_4584 NC_008611.1 5082945 5084279 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 5082945..5084279 Mycobacterium ulcerans Agy99 4553069 YP_908008.1 CDS MUL_4585 NC_008611.1 5084584 5085630 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(5084584..5085630) Mycobacterium ulcerans Agy99 4553070 YP_908009.1 CDS MUL_4586 NC_008611.1 5086047 5087390 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 5086047..5087390 Mycobacterium ulcerans Agy99 4553071 YP_908010.1 CDS MUL_4587 NC_008611.1 5087447 5088493 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(5087447..5088493) Mycobacterium ulcerans Agy99 4553072 YP_908011.1 CDS MUL_4589 NC_008611.1 5089459 5090505 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(5089459..5090505) Mycobacterium ulcerans Agy99 4553074 YP_908012.1 CDS MUL_4590 NC_008611.1 5090934 5091221 D cytoplasmic protein; hypothetical protein 5090934..5091221 Mycobacterium ulcerans Agy99 4553075 YP_908013.1 CDS MUL_4591 NC_008611.1 5091355 5091669 D cytoplasmic protein; hypothetical protein 5091355..5091669 Mycobacterium ulcerans Agy99 4553076 YP_908014.1 CDS MUL_4592 NC_008611.1 5091788 5092387 D cytoplasmic protein; hypothetical protein 5091788..5092387 Mycobacterium ulcerans Agy99 4553077 YP_908015.1 CDS MUL_4594 NC_008611.1 5094418 5095200 R cytoplasmic protein; hypothetical protein complement(5094418..5095200) Mycobacterium ulcerans Agy99 4554528 YP_908016.1 CDS MUL_4595 NC_008611.1 5095580 5096626 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(5095580..5096626) Mycobacterium ulcerans Agy99 4554529 YP_908017.1 CDS MUL_4597 NC_008611.1 5097918 5098187 D cytoplasmic protein; hypothetical protein 5097918..5098187 Mycobacterium ulcerans Agy99 4554531 YP_908018.1 CDS hemA NC_008611.1 5098297 5099718 D catalyzes the formation of glutamate-1-semialdehyde from glutamyl-tRNA(Glu) and NADPH; the second step of the pathway is catalyzed by glutamate-1-semialdehyde aminomutase which results in the formation of 5-aminolevulinic acid; functions in porphyrin (tetrapyrroles) biosynthesis; the crystal structure showed a C-terminal dimerization domain that appears to be absent in Chlamydial proteins; glutamyl-tRNA reductase 5098297..5099718 Mycobacterium ulcerans Agy99 4554532 YP_908019.1 CDS hemC NC_008611.1 5099732 5100676 D transformation of porphobilinogen to hydroxymethylbilane in porphyrin biosynthesis; porphobilinogen deaminase 5099732..5100676 Mycobacterium ulcerans Agy99 4554533 YP_908020.1 CDS hemD NC_008611.1 5100701 5102374 D cytoplasmic protein; possibly involved in the biosynthesis of siroheme and cobalamin [catalytic activity: 2 S-adenosyl-L- methionine + uroporphyrin III = 2 S-adenosyl-L- homocysteine + sirohydrochlorin]; uroporphyrin-III C-methyltransferase HemD 5100701..5102374 Mycobacterium ulcerans Agy99 4554534 YP_908021.1 CDS hemB NC_008611.1 5102414 5103397 D catalyzes the formation of porphobilinogen from 5-aminolevulinate; delta-aminolevulinic acid dehydratase 5102414..5103397 Mycobacterium ulcerans Agy99 4554535 YP_908022.1 CDS MUL_4602 NC_008611.1 5103409 5103945 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; transmembrane protein 5103409..5103945 Mycobacterium ulcerans Agy99 4554536 YP_908023.1 CDS MUL_4603 NC_008611.1 5103942 5104226 D membrane protein; transmembrane protein 5103942..5104226 Mycobacterium ulcerans Agy99 4554537 YP_908024.1 CDS MUL_4605 NC_008611.1 5104448 5104561 R hypothetical protein complement(5104448..5104561) Mycobacterium ulcerans Agy99 4554538 YP_908025.1 CDS MUL_4606 NC_008611.1 5104688 5106022 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 5104688..5106022 Mycobacterium ulcerans Agy99 4554539 YP_908026.1 CDS MUL_4607 NC_008611.1 5106413 5107459 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 5106413..5107459 Mycobacterium ulcerans Agy99 4554540 YP_908027.1 CDS MUL_4611 NC_008611.1 5112123 5113169 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 5112123..5113169 Mycobacterium ulcerans Agy99 4552220 YP_908028.1 CDS MUL_4612 NC_008611.1 5113285 5113521 R membrane protein; hypothetical protein complement(5113285..5113521) Mycobacterium ulcerans Agy99 4552221 YP_908029.1 CDS MUL_4613 NC_008611.1 5113790 5115124 D cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 5113790..5115124 Mycobacterium ulcerans Agy99 4552222 YP_908030.1 CDS MUL_4614 NC_008611.1 5115515 5116561 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 5115515..5116561 Mycobacterium ulcerans Agy99 4552223 YP_908031.1 CDS MUL_4615 NC_008611.1 5116647 5117168 D membrane protein; hypothetical protein 5116647..5117168 Mycobacterium ulcerans Agy99 4552224 YP_908032.1 CDS MUL_4616 NC_008611.1 5117335 5118039 D membrane protein; hypothetical protein 5117335..5118039 Mycobacterium ulcerans Agy99 4552225 YP_908033.1 CDS MUL_4617 NC_008611.1 5118178 5119707 R membrane protein; hypothetical protein complement(5118178..5119707) Mycobacterium ulcerans Agy99 4552226 YP_908034.1 CDS MUL_4620 NC_008611.1 5120301 5121125 D PE8; membrane protein; PE family protein 5120301..5121125 Mycobacterium ulcerans Agy99 4552228 YP_908035.1 CDS MUL_4621 NC_008611.1 5121195 5122367 D PPE15; membrane protein; PPE family protein 5121195..5122367 Mycobacterium ulcerans Agy99 4552229 YP_908036.1 CDS esxP NC_008611.1 5122515 5122811 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; EsaT-6 like protein EsxP 5122515..5122811 Mycobacterium ulcerans Agy99 4552230 YP_908037.1 CDS esxN_2 NC_008611.1 5122850 5123134 D Also detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; EsaT-6 like protein EsxN_2 5122850..5123134 Mycobacterium ulcerans Agy99 4552231 YP_908038.1 CDS trcR NC_008611.1 5123395 5124168 D cytoplasmic protein; sensor part of the two component regulatory system TrcS/TrcR. involved in transcriptional autoactivation: TrcR activates its own expression by interacting with the AT-rich sequence of the TrcR promoter.; two component transcriptional regulator TrcR 5123395..5124168 Mycobacterium ulcerans Agy99 4552232 YP_908039.1 CDS trcS NC_008611.1 5124218 5125720 D membrane protein; sensor part of the two component regulatory system TrcS/TrcR.; two component sensor histidine kinase TrcS 5124218..5125720 Mycobacterium ulcerans Agy99 4552233 YP_908040.1 CDS MUL_4626 NC_008611.1 5125800 5126498 D cytoplasmic protein; function unknown, possibly involved in amino acid transport and metabolism.; hydantoin racemase 5125800..5126498 Mycobacterium ulcerans Agy99 4552234 YP_908041.1 CDS MUL_4627 NC_008611.1 5126585 5126878 R cytoplasmic protein; hypothetical protein complement(5126585..5126878) Mycobacterium ulcerans Agy99 4552235 YP_908042.1 CDS MUL_4628 NC_008611.1 5126982 5127884 R cytoplasmic protein; function unknown; identity to GppA, exopolyphosphatase [nucleotide transport and metabolism / inorganic ion transport and metabolism]; hypothetical protein complement(5126982..5127884) Mycobacterium ulcerans Agy99 4550799 YP_908043.1 CDS MUL_4629 NC_008611.1 5127945 5128436 R cytoplasmic protein; hypothetical protein complement(5127945..5128436) Mycobacterium ulcerans Agy99 4550800 YP_908044.1 CDS MUL_4630 NC_008611.1 5128429 5129130 R membrane protein; hypothetical protein complement(5128429..5129130) Mycobacterium ulcerans Agy99 4550801 YP_908045.1 CDS eno NC_008611.1 5129164 5130450 R enolase; catalyzes the formation of phosphoenolpyruvate from 2-phospho-D-glycerate in glycolysis; phosphopyruvate hydratase complement(5129164..5130450) Mycobacterium ulcerans Agy99 4550802 YP_908046.1 CDS lpqU NC_008611.1 5130531 5131262 R secreted protein; lipoprotein LpqU complement(5130531..5131262) Mycobacterium ulcerans Agy99 4550803 YP_908047.1 CDS MUL_4633 NC_008611.1 5131617 5132717 R functions in degradation of stringent response intracellular messenger ppGpp; in Escherichia coli this gene is co-transcribed with the toxin/antitoxin genes mazEF; activity of MazG is inhibited by MazEF in vitro; ppGpp inhibits mazEF expression; MazG thus works in limiting the toxic activity of the MazF toxin induced during starvation; MazG also interacts with the GTPase protein Era; nucleoside triphosphate pyrophosphohydrolase complement(5131617..5132717) Mycobacterium ulcerans Agy99 4550804 YP_908048.1 CDS mfd NC_008611.1 5132714 5136382 R cytoplasmic protein; involved in nucleotide excision repair. necessary for strand-specific repair. a lesion in the template strand blocks the RNA polymerase complex (RNAP) the RNAP- DNA-RNA complex is specifically recognized by TrcF which releases RNAP and the truncated transcript; the TcrF may replace RNAP at the lesion site and then recruit the UvrA/B/C repair system.; transcription-repair coupling factor Mfd (TrcF) complement(5132714..5136382) Mycobacterium ulcerans Agy99 4550805 YP_908049.1 CDS MUL_4635 NC_008611.1 5136449 5137042 R cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator complement(5136449..5137042) Mycobacterium ulcerans Agy99 4550806 YP_908050.1 CDS glmU NC_008611.1 5137519 5138997 D forms a homotrimer; catalyzes the acetylation of glucosamine-1-phosphate and uridylation of N-acetylglucosamine-1-phosphate to produce UDP-GlcNAc; function in cell wall synthesis; bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase 5137519..5138997 Mycobacterium ulcerans Agy99 4550808 YP_908051.1 CDS prsA NC_008611.1 5139110 5140090 D catalyzes the formation of 5-phospho-alpha-D-ribose 1-phosphate from D-ribose 5-phosphate and ATP; ribose-phosphate pyrophosphokinase 5139110..5140090 Mycobacterium ulcerans Agy99 4550809 YP_908052.1 CDS MUL_4639 NC_008611.1 5140091 5140459 D cytoplasmic protein; arsenate reductase 5140091..5140459 Mycobacterium ulcerans Agy99 4550810 YP_908053.1 CDS lpqT NC_008611.1 5140456 5141112 D Also detected in the membrane fraction by proteomics (LC-MS/MS); secreted protein; lipoprotein LpqT 5140456..5141112 Mycobacterium ulcerans Agy99 4550811 YP_908054.1 CDS MUL_4642 NC_008611.1 5141854 5142900 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(5141854..5142900) Mycobacterium ulcerans Agy99 4550813 YP_908055.1 CDS rplY NC_008611.1 5143598 5144242 D the Ctc family of proteins consists of two types, one that contains the N-terminal ribosomal protein L25 domain only which in Escherichia coli binds the 5S rRNA while a subset of proteins contain a C-terminal extension that is involved in the stress response; 50S ribosomal protein L25/general stress protein Ctc 5143598..5144242 Mycobacterium ulcerans Agy99 4550814 YP_908056.1 CDS pth NC_008611.1 5144255 5144830 D Enables the recycling of peptidyl-tRNAs produced at termination of translation; peptidyl-tRNA hydrolase 5144255..5144830 Mycobacterium ulcerans Agy99 4550815 YP_908057.1 CDS MUL_4645 NC_008611.1 5144844 5145758 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(5144844..5145758) Mycobacterium ulcerans Agy99 4554492 YP_908058.1 CDS pks16 NC_008611.1 5147613 5149247 R activates fatty acids by binding to coenzyme A; long-chain-fatty-acid--CoA ligase complement(5147613..5149247) Mycobacterium ulcerans Agy99 4554494 YP_908059.1 CDS ispE NC_008611.1 5149613 5150536 R catalyzes the phosphorylation of 4-diphosphocytidyl-2-C-methyl-D-erythritol in the nonmevalonate pathway of isoprenoid biosynthesis; 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase complement(5149613..5150536) Mycobacterium ulcerans Agy99 4554495 YP_908060.1 CDS ksgA NC_008611.1 5150634 5151458 R catalyzes the transfer of a total of four methyl groups from S-adenosyl-l-methionine (S-AdoMet) to two adjacent adenosine bases A1518 and A1519 in 16S rRNA; mutations in ksgA causes resistance to the translation initiation inhibitor kasugamycin; dimethyladenosine transferase complement(5150634..5151458) Mycobacterium ulcerans Agy99 4554496 YP_908061.1 CDS rpfB NC_008611.1 5151563 5152651 R secreted protein; thought to promote the resuscitation and growth of dormant, nongrowing cell. could also stimulates the growth of several other high G+C Gram+ organisms, E.G. mycobacterium avium, mycobacterium bovis (BCG), mycobacterium kansasii, mycobacterium smegmatis.; resuscitation-promoting factor RpfB complement(5151563..5152651) Mycobacterium ulcerans Agy99 4554497 YP_908062.1 CDS tatD NC_008611.1 5152861 5153643 R membrane protein; DNAse involved in proteins export. this sec- independent pathway is termed tat for twin-arginine translocation system. this system mainly transports proteins with bound cofactors that require folding prior to export (by similarity); deoxyribonuclease TatD complement(5152861..5153643) Mycobacterium ulcerans Agy99 4554498 YP_908063.1 CDS metS NC_008611.1 5153743 5155299 D methionine--tRNA ligase; MetRS; adds methionine to tRNA(Met) with cleavage of ATP to AMP and diphosphate; some MetRS enzymes form dimers depending on a C-terminal domain that is also found in other proteins such as Trbp111 in Aquifex aeolicus and the cold-shock protein CsaA from Bacillus subtilis while others do not; four subfamilies exist based on sequence motifs and zinc content; methionyl-tRNA synthetase 5153743..5155299 Mycobacterium ulcerans Agy99 4554499 YP_908064.1 CDS cysE_1 NC_008611.1 5155583 5156149 D cytoplasmic protein; involved in cysteine biosynthesis [catalytic activity: acetyl-CoA + L-serine = CoA + O-acetyl-L- serine]; serine acetyltransferase CysE 5155583..5156149 Mycobacterium ulcerans Agy99 4554500 YP_908065.1 CDS MUL_4658 NC_008611.1 5158419 5159603 D cytoplasmic protein; function unknown, probably involved in cellular metabolism; dehydrogenase 5158419..5159603 Mycobacterium ulcerans Agy99 4554502 YP_908066.1 CDS pabD NC_008611.1 5160569 5161882 D catalyzes the formation of 4-amino-4-deoxychorismate from chorismate and glutamine; aminodeoxychorismate synthase component I 5160569..5161882 Mycobacterium ulcerans Agy99 4554504 YP_908067.1 CDS MUL_4662 NC_008611.1 5163343 5164197 R cytoplasmic protein; hypothetical protein complement(5163343..5164197) Mycobacterium ulcerans Agy99 4554506 YP_908068.1 CDS MUL_4663 NC_008611.1 5164196 5165779 D membrane protein; hypothetical protein 5164196..5165779 Mycobacterium ulcerans Agy99 4554507 YP_908069.1 CDS arcA NC_008611.1 5165776 5166984 R catalyzes the degradation of arginine to citruline and ammonia; arginine deiminase complement(5165776..5166984) Mycobacterium ulcerans Agy99 4554508 YP_908070.1 CDS MUL_4665 NC_008611.1 5167043 5167660 D cytoplasmic protein; hypothetical protein 5167043..5167660 Mycobacterium ulcerans Agy99 4551204 YP_908071.1 CDS MUL_4666 NC_008611.1 5167686 5168339 R secreted protein; hypothetical protein complement(5167686..5168339) Mycobacterium ulcerans Agy99 4551205 YP_908072.1 CDS MUL_5094 NC_008611.1 5168367 5169044 R secreted protein; hypothetical protein complement(5168367..5169044) Mycobacterium ulcerans Agy99 4551206 YP_908073.1 CDS MUL_4667 NC_008611.1 5169116 5170117 R cytoplasmic protein; hypothetical protein complement(5169116..5170117) Mycobacterium ulcerans Agy99 4551207 YP_908074.1 CDS MUL_4668 NC_008611.1 5170282 5171028 D cytoplasmic protein; thought to cause methylation.; methyltransferase 5170282..5171028 Mycobacterium ulcerans Agy99 4551208 YP_908075.1 CDS MUL_4669 NC_008611.1 5171064 5171315 R cytoplasmic protein; hypothetical protein complement(5171064..5171315) Mycobacterium ulcerans Agy99 4551209 YP_908076.1 CDS MUL_4670 NC_008611.1 5171430 5172167 R membrane protein; hypothetical protein complement(5171430..5172167) Mycobacterium ulcerans Agy99 4551210 YP_908077.1 CDS MUL_4671 NC_008611.1 5172293 5172829 D membrane protein; hypothetical protein 5172293..5172829 Mycobacterium ulcerans Agy99 4551211 YP_908078.1 CDS MUL_4672 NC_008611.1 5173146 5174318 D membrane protein; PPE family protein 5173146..5174318 Mycobacterium ulcerans Agy99 4551212 YP_908079.1 CDS MUL_4673 NC_008611.1 5174543 5175373 D cytoplasmic protein; function unknown, possibly involved in cell envelope biogenesis, outer membrane; teichoic acid biosynthesis protein 5174543..5175373 Mycobacterium ulcerans Agy99 4551213 YP_908080.1 CDS MUL_4674 NC_008611.1 5175390 5176895 D membrane protein; function unknown, possibly involved in lipopolysaccharide synthesis.; hypothetical protein 5175390..5176895 Mycobacterium ulcerans Agy99 4551214 YP_908081.1 CDS MUL_4675 NC_008611.1 5176910 5178385 D membrane protein; function unknown, possible membrane protein involved in the export of O-antigen and teichoic acid.; hypothetical protein 5176910..5178385 Mycobacterium ulcerans Agy99 4551215 YP_908082.1 CDS MUL_4676 NC_008611.1 5178382 5180373 D cytoplasmic protein; probably involved in cell wall synthesis; glycosyltransferase 5178382..5180373 Mycobacterium ulcerans Agy99 4551216 YP_908083.1 CDS MUL_4677 NC_008611.1 5180370 5181425 D cytoplasmic protein; function unknown, contains RfaG, glycosyltransferase domain. may be involved in cell envelope biogenesis, outer membrane.; hypothetical protein 5180370..5181425 Mycobacterium ulcerans Agy99 4551217 YP_908084.1 CDS MUL_4678 NC_008611.1 5181440 5182390 R cytoplasmic protein; hypothetical protein complement(5181440..5182390) Mycobacterium ulcerans Agy99 4551218 YP_908085.1 CDS MUL_4679 NC_008611.1 5182443 5184404 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(5182443..5184404) Mycobacterium ulcerans Agy99 4551219 YP_908086.1 CDS MUL_4680 NC_008611.1 5184841 5185800 D cytoplasmic protein; hypothetical protein 5184841..5185800 Mycobacterium ulcerans Agy99 4551220 YP_908087.1 CDS MUL_4681 NC_008611.1 5185780 5186577 R cytoplasmic protein; hypothetical protein complement(5185780..5186577) Mycobacterium ulcerans Agy99 4551221 YP_908088.1 CDS MUL_4682 NC_008611.1 5186678 5187343 R cytoplasmic protein; hypothetical protein complement(5186678..5187343) Mycobacterium ulcerans Agy99 4551222 YP_908089.1 CDS MUL_4683 NC_008611.1 5187502 5188161 D cytoplasmic protein; function unknown; hydrolytic enzyme probably involved in cellular metabolism.; amidohydrolase 5187502..5188161 Mycobacterium ulcerans Agy99 4554370 YP_908090.1 CDS MUL_4684 NC_008611.1 5188238 5188999 R cytoplasmic protein; hypothetical protein complement(5188238..5188999) Mycobacterium ulcerans Agy99 4554371 YP_908091.1 CDS MUL_4686 NC_008611.1 5190210 5191685 R membrane protein; hypothetical protein complement(5190210..5191685) Mycobacterium ulcerans Agy99 4554373 YP_908092.1 CDS MUL_4688 NC_008611.1 5193402 5194184 D cytoplasmic protein; hypothetical protein 5193402..5194184 Mycobacterium ulcerans Agy99 4554375 YP_908093.1 CDS MUL_4690 NC_008611.1 5194419 5195597 R membrane protein; hypothetical protein complement(5194419..5195597) Mycobacterium ulcerans Agy99 4554377 YP_908094.1 CDS rimJ NC_008611.1 5195805 5196416 R cytoplasmic protein; this enzyme acetylates the N-terminal alanine of ribosomal protein S5 [catalytic activity: acetyl-CoA + ribosomal-protein L-alanine = CoA + ribosomal-protein N- acetyl-L-alanine]; ribosomal-protein-alanine acetyltransferase RimJ complement(5195805..5196416) Mycobacterium ulcerans Agy99 4554378 YP_908095.1 CDS moeA1 NC_008611.1 5196481 5197770 R cytoplasmic protein; involved in molybdenum cofactor biosynthesis: involved in the biosynthesis of a demolybdo-cofactor (molybdopterin), necessary for molybdo-enzymes.; molybdopterin biosynthesis protein MoeA1 complement(5196481..5197770) Mycobacterium ulcerans Agy99 4554379 YP_908096.1 CDS galU NC_008611.1 5197822 5198739 R cytoplasmic protein; may play a role in stationary phase survival [catalytic activity: UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose]; UTP--glucose-1-phosphate uridylyltransferase GalU complement(5197822..5198739) Mycobacterium ulcerans Agy99 4554380 YP_908097.1 CDS MUL_4694 NC_008611.1 5198817 5199410 D cytoplasmic protein; hypothetical protein 5198817..5199410 Mycobacterium ulcerans Agy99 4554381 YP_908098.1 CDS MUL_4695 NC_008611.1 5199485 5199820 D cytoplasmic protein; putative serine rich protein 5199485..5199820 Mycobacterium ulcerans Agy99 4554382 YP_908099.1 CDS MUL_4696 NC_008611.1 5199887 5200561 D membrane protein; hypothetical protein 5199887..5200561 Mycobacterium ulcerans Agy99 4554383 YP_908100.1 CDS mscL NC_008611.1 5200638 5201093 D forms homopentamer; channel that opens in response to pressure or hypoosmotic shock; large-conductance mechanosensitive channel 5200638..5201093 Mycobacterium ulcerans Agy99 4554384 YP_908101.1 CDS moaB2 NC_008611.1 5201113 5201658 R Detected in the cytoplasmic and secreted fractions by proteomics.; cytoplasmic protein; thought to be involved in molybdopterin biosynthesis. catalyzes the dehydratation of 4A- hydroxytetrahydropterins [catalytic activity: (6R)-6-(L- erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro -4 A- hydroxypterin = (6R)-6-(L-erythro-1,2- dihydroxypropyl)- 7,8-dihydro-6H-pterin + H(2)O]; pterin-4-alpha-carbinolamine dehydratase MoaB2 complement(5201113..5201658) Mycobacterium ulcerans Agy99 4554385 YP_908102.1 CDS pepD NC_008611.1 5201655 5203094 R Non-secretory protein; membrane protein; function unknown, possibly hydrolyzes peptides and/or proteins (seems to cleave preferentially after serine residues); serine protease PepD complement(5201655..5203094) Mycobacterium ulcerans Agy99 4554386 YP_908103.1 CDS mprB NC_008611.1 5203195 5204730 R membrane protein; sensor part of a two component regulatory system (supposed MprAB system); two component sensor kinase MprB complement(5203195..5204730) Mycobacterium ulcerans Agy99 4554387 YP_908104.1 CDS mprA NC_008611.1 5204730 5205428 R cytoplasmic protein; regulator part of a two component regulatory system (supposed MprAB system); two component response transcriptional regulatory protein MprA complement(5204730..5205428) Mycobacterium ulcerans Agy99 4554298 YP_908105.1 CDS rpmF NC_008611.1 5205695 5205868 R some L32 proteins have zinc finger motifs consisting of CXXC while others do not; 50S ribosomal protein L32 complement(5205695..5205868) Mycobacterium ulcerans Agy99 4554299 YP_908106.1 CDS MUL_4703 NC_008611.1 5206134 5207846 D cytoplasmic protein; hypothetical protein 5206134..5207846 Mycobacterium ulcerans Agy99 4554300 YP_908107.1 CDS fadE13 NC_008611.1 5207843 5208991 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE13 5207843..5208991 Mycobacterium ulcerans Agy99 4554301 YP_908108.1 CDS accD2 NC_008611.1 5209035 5210636 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in fatty acid metabolism.; acetyl-/propionyl-CoA carboxylase subunit beta 5209035..5210636 Mycobacterium ulcerans Agy99 4554302 YP_908109.1 CDS accA2 NC_008611.1 5210642 5212624 D Detected in the membrane fraction by proteomics (2D- LC-MS/MS); cytoplasmic protein; this protein carries two functions: biotin carboxyl carrier protein and biotin carboxyltransferase. involved in the first step of long-chain fatty acid synthesis [catalytic activity: ATP + biotin-carboxyl-carrier protein + CO(2) = ADP + phosphate + carboxybiotin-carboxyl-carrier protein]; acetyl-/propionyl-coenzyme a carboxylase alpha chain, AccA2 5210642..5212624 Mycobacterium ulcerans Agy99 4554303 YP_908110.1 CDS fadE12 NC_008611.1 5212621 5213790 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown, but involved in lipid metabolism.; acyl-CoA dehydrogenase FadE12 5212621..5213790 Mycobacterium ulcerans Agy99 4554304 YP_908111.1 CDS echA7 NC_008611.1 5213801 5214574 D Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase 5213801..5214574 Mycobacterium ulcerans Agy99 4554305 YP_908112.1 CDS MUL_4709 NC_008611.1 5215037 5215639 D cytoplasmic protein; hypothetical protein 5215037..5215639 Mycobacterium ulcerans Agy99 4554307 YP_908113.1 CDS MUL_4710 NC_008611.1 5215667 5217664 R cytoplasmic protein; hypothetical protein complement(5215667..5217664) Mycobacterium ulcerans Agy99 4554308 YP_908114.1 CDS MUL_4711 NC_008611.1 5217657 5219051 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; chelation, introducing a magnesium ion into specific substrate.; magnesium chelatase complement(5217657..5219051) Mycobacterium ulcerans Agy99 4554309 YP_908115.1 CDS purH NC_008611.1 5219128 5220699 R involved in de novo purine biosynthesis; bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase complement(5219128..5220699) Mycobacterium ulcerans Agy99 4554310 YP_908116.1 CDS purN NC_008611.1 5220696 5221343 R glycinamide ribonucleotide transformylase; GAR Tfase; catalyzes the synthesis of 5'-phosphoribosylformylglycinamide from 5'-phosphoribosylglycinamide and 10-formyltetrahydrofolate; PurN requires formyl folate for the reaction unlike PurT which uses formate; phosphoribosylglycinamide formyltransferase complement(5220696..5221343) Mycobacterium ulcerans Agy99 4554311 YP_908117.1 CDS MUL_4714 NC_008611.1 5221396 5222787 R membrane protein; integral membrane protein complement(5221396..5222787) Mycobacterium ulcerans Agy99 4554312 YP_908118.1 CDS MUL_4715 NC_008611.1 5222836 5223741 R membrane protein; transmembrane protein complement(5222836..5223741) Mycobacterium ulcerans Agy99 4554313 YP_908119.1 CDS MUL_4717 NC_008611.1 5224819 5226339 R Catalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation; acetyl-CoA acetyltransferase complement(5224819..5226339) Mycobacterium ulcerans Agy99 4554315 YP_908120.1 CDS sucD NC_008611.1 5227878 5228780 R Catalyzes the only substrate-level phosphorylation in the TCA cycle; succinyl-CoA synthetase subunit alpha complement(5227878..5228780) Mycobacterium ulcerans Agy99 4551289 YP_908121.1 CDS sucC NC_008611.1 5228796 5229959 R catalyzes the interconversion of succinyl-CoA and succinate; succinyl-CoA synthetase subunit beta complement(5228796..5229959) Mycobacterium ulcerans Agy99 4551290 YP_908122.1 CDS MUL_4721 NC_008611.1 5230235 5231293 D cytoplasmic protein; hypothetical protein 5230235..5231293 Mycobacterium ulcerans Agy99 4551291 YP_908123.1 CDS MUL_4722 NC_008611.1 5231297 5231881 D membrane protein; hypothetical protein 5231297..5231881 Mycobacterium ulcerans Agy99 4551292 YP_908124.1 CDS uvrD1 NC_008611.1 5231960 5234269 R cytoplasmic protein; involved in nucleotide excision repair. has a 3'-5' helicase activity in presence of ATP. preferred substrate being one with both single and double stranded regions of DNA.; ATP-dependent DNA helicase II UvrD1 complement(5231960..5234269) Mycobacterium ulcerans Agy99 4551293 YP_908125.1 CDS MUL_4725 NC_008611.1 5234751 5235809 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(5234751..5235809) Mycobacterium ulcerans Agy99 4551295 YP_908126.1 CDS MUL_4727 NC_008611.1 5237281 5238615 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(5237281..5238615) Mycobacterium ulcerans Agy99 4551297 YP_908127.1 CDS MUL_4729 NC_008611.1 5238926 5239972 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(5238926..5239972) Mycobacterium ulcerans Agy99 4551299 YP_908128.1 CDS MUL_4731 NC_008611.1 5240872 5241495 R cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator complement(5240872..5241495) Mycobacterium ulcerans Agy99 4551300 YP_908129.1 CDS MUL_4732 NC_008611.1 5241471 5241788 D fragment; cytoplasmic protein; hypothetical protein 5241471..5241788 Mycobacterium ulcerans Agy99 4551301 YP_908130.1 CDS pntB NC_008611.1 5241831 5243255 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; the transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [catalytic activity: NADPH + NAD+ = NADP+ + NADH]; NAD(P) transhydrogenasePntB complement(5241831..5243255) Mycobacterium ulcerans Agy99 4551302 YP_908131.1 CDS pntAb NC_008611.1 5243252 5243590 R membrane protein; the transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [catalytic activity: NADPH + NAD+ = NADP+ + NADH]; NAD(P) transhydrogenase (subunit alpha) PntAb complement(5243252..5243590) Mycobacterium ulcerans Agy99 4551303 YP_908132.1 CDS pntAa NC_008611.1 5243606 5244694 R Detected in the cytoplasmic and secreted fractions by 2D-LC-MS/MS.; cytoplasmic protein; the transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [catalytic activity: NADPH + NAD+ = NADP+ + NADH]; NAD(P) transhydrogenase PntA complement(5243606..5244694) Mycobacterium ulcerans Agy99 4551322 YP_908133.1 CDS MUL_4736 NC_008611.1 5244938 5245408 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(5244938..5245408) Mycobacterium ulcerans Agy99 4551323 YP_908134.1 CDS MUL_4737 NC_008611.1 5245474 5246352 R cytoplasmic protein; hypothetical protein complement(5245474..5246352) Mycobacterium ulcerans Agy99 4551324 YP_908135.1 CDS ptpB NC_008611.1 5247721 5248551 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in signal transduction (via dephosphorylation) can dephosphorylated in vitro the phosphotyrosine residue of myelin basic protein (MBP) at ph 7.0. could be involved in virulence by interfering with phosphotyrosine-mediated signals in macrophages [catalytic activity: protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate]; phosphotyrosine protein phosphatase PtpB 5247721..5248551 Mycobacterium ulcerans Agy99 4551326 YP_908136.1 CDS MUL_4741 NC_008611.1 5248870 5249256 D cytoplasmic protein; hypothetical protein 5248870..5249256 Mycobacterium ulcerans Agy99 4551327 YP_908137.1 CDS MUL_4742 NC_008611.1 5249304 5250032 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein complement(5249304..5250032) Mycobacterium ulcerans Agy99 4551328 YP_908138.1 CDS MUL_4744 NC_008611.1 5251308 5252186 D predicted by Cello and psort. Detected in the secreted fraction by 2D-LC-MS-MS.; secreted protein; possibly hydrolyzes specific sugar (hydrolyzation of glycosidic bond) and could be involved in exopolysaccharide biosynthesis/degradation. could also have a lytic activity against cell walls.; beta-1,3-glucanase 5251308..5252186 Mycobacterium ulcerans Agy99 4551330 YP_908139.1 CDS MUL_4746 NC_008611.1 5252601 5252918 R cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 complement(5252601..5252918) Mycobacterium ulcerans Agy99 4551332 YP_908140.1 CDS MUL_4747 NC_008611.1 5252943 5253647 R cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 complement(5252943..5253647) Mycobacterium ulcerans Agy99 4551333 YP_908141.1 CDS MUL_4749 NC_008611.1 5259211 5260257 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(5259211..5260257) Mycobacterium ulcerans Agy99 4551335 YP_908142.1 CDS MUL_4751 NC_008611.1 5266112 5266429 R cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 complement(5266112..5266429) Mycobacterium ulcerans Agy99 4551337 YP_908143.1 CDS MUL_4752 NC_008611.1 5266454 5267158 R cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 complement(5266454..5267158) Mycobacterium ulcerans Agy99 4550889 YP_908144.1 CDS pknK_3 NC_008611.1 5267954 5270893 R membrane protein; involved in signal transduction (via phosphorylation) involved in transcriptional regulatory mechanism and in the regulation of secondary metabolites [catalytic activity: ATP + a protein = ADP + a phosphoprotein]; serine/threonine-protein kinase transcriptional regulatory protein PknK_3 complement(5267954..5270893) Mycobacterium ulcerans Agy99 4550890 YP_908145.1 CDS pknK_2 NC_008611.1 5271118 5274102 R membrane protein; involved in signal transduction (via phosphorylation) involved in transcriptional regulatory mechanism and in the regulation of secondary metabolites [catalytic activity: ATP + a protein = ADP + a phosphoprotein]; serine/threonine-protein kinase transcriptional regulatory protein PknK_2 complement(5271118..5274102) Mycobacterium ulcerans Agy99 4550891 YP_908146.1 CDS MUL_4755 NC_008611.1 5274163 5274381 R cytoplasmic protein; hypothetical protein complement(5274163..5274381) Mycobacterium ulcerans Agy99 4550892 YP_908147.1 CDS MUL_4756 NC_008611.1 5274750 5275286 D membrane protein; hypothetical protein 5274750..5275286 Mycobacterium ulcerans Agy99 4550893 YP_908148.1 CDS MUL_4757 NC_008611.1 5275312 5275668 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein 5275312..5275668 Mycobacterium ulcerans Agy99 4550894 YP_908149.1 CDS MUL_4758 NC_008611.1 5275750 5276718 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; possibly binds NADP and acts through a one-electron transfer process. quinones are supposed to be the best substrates. may act in the detoxification of xenobiotics [catalytic activity: NADPH + quinone = NADP+ + semiquinone]; quinone oxidoreductase complement(5275750..5276718) Mycobacterium ulcerans Agy99 4550895 YP_908150.1 CDS MUL_4759 NC_008611.1 5276723 5277583 R Found in the cytoplasmic and membrane fractions by LC-MS/MS. Also detected in the secreted protein and extracellular matrix fractions by proteomics.; cytoplasmic protein; function unknown, possibly involved in cellular metabolism.; short-chain type dehydrogenase/reductase complement(5276723..5277583) Mycobacterium ulcerans Agy99 4550896 YP_908151.1 CDS MUL_4760 NC_008611.1 5277656 5279140 R Detected in the membrane and the cytoplasmic fractions by proteomics.; cytoplasmic protein; function unknown, probably involved in cellular metabolism [catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH]; aldehyde dehydrogenase (NAD+) dependent complement(5277656..5279140) Mycobacterium ulcerans Agy99 4550897 YP_908152.1 CDS MUL_4761 NC_008611.1 5279290 5280156 R cytoplasmic protein; O-methyltransferase complement(5279290..5280156) Mycobacterium ulcerans Agy99 4550898 YP_908153.1 CDS MUL_4762 NC_008611.1 5280226 5281158 R cytoplasmic protein; O-methyltransferase complement(5280226..5281158) Mycobacterium ulcerans Agy99 4550899 YP_908154.1 CDS MUL_4763 NC_008611.1 5281181 5282113 R cytoplasmic protein; hypothetical protein complement(5281181..5282113) Mycobacterium ulcerans Agy99 4550900 YP_908155.1 CDS MUL_4764 NC_008611.1 5282130 5282237 R cytoplasmic protein; hypothetical protein complement(5282130..5282237) Mycobacterium ulcerans Agy99 4550901 YP_908156.1 CDS MUL_4765 NC_008611.1 5282258 5282896 R cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator complement(5282258..5282896) Mycobacterium ulcerans Agy99 4550902 YP_908157.1 CDS MUL_4766 NC_008611.1 5282893 5283897 R Detected in the cytoplasmic fraction by proteomics.; cytoplasmic protein; hypothetical protein complement(5282893..5283897) Mycobacterium ulcerans Agy99 4550903 YP_908158.1 CDS MUL_4769 NC_008611.1 5286515 5286916 D cytoplasmic protein; hypothetical protein 5286515..5286916 Mycobacterium ulcerans Agy99 4550906 YP_908159.1 CDS MUL_4770 NC_008611.1 5286903 5287283 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; hypothetical protein complement(5286903..5287283) Mycobacterium ulcerans Agy99 4550907 YP_908160.1 CDS MUL_4771 NC_008611.1 5287393 5288415 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase complement(5287393..5288415) Mycobacterium ulcerans Agy99 4550908 YP_908161.1 CDS MUL_4772 NC_008611.1 5288412 5288918 R cytoplasmic protein; hypothetical protein complement(5288412..5288918) Mycobacterium ulcerans Agy99 4550007 YP_908162.1 CDS msrA NC_008611.1 5289078 5289593 D this stereospecific enzymes reduces the S isomer of methionine sulfoxide while MsrB reduces the R form; provides protection against oxidative stress; methionine sulfoxide reductase A 5289078..5289593 Mycobacterium ulcerans Agy99 4550008 YP_908163.1 CDS MUL_4775 NC_008611.1 5291084 5291689 D cytoplasmic protein; could be involved in transcriptional mechanism.; transcriptional regulatory protein 5291084..5291689 Mycobacterium ulcerans Agy99 4550010 YP_908164.1 CDS MUL_4776 NC_008611.1 5291735 5291947 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); cytoplasmic protein; hypothetical protein 5291735..5291947 Mycobacterium ulcerans Agy99 4550011 YP_908165.1 CDS MUL_4777 NC_008611.1 5291954 5292997 D cytoplasmic protein; oxido-reduction; zinc-dependent alcohol dehydrogenase 5291954..5292997 Mycobacterium ulcerans Agy99 4550012 YP_908166.1 CDS ephF NC_008611.1 5293061 5293951 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; thought to be involved in detoxification reactions following oxidative damage to lipids [catalytic activity: an epoxide + H(2)O = a glycol]; epoxide hydrolase EphF complement(5293061..5293951) Mycobacterium ulcerans Agy99 4550013 YP_908167.1 CDS MUL_4780 NC_008611.1 5295261 5295860 D cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator 5295261..5295860 Mycobacterium ulcerans Agy99 4550015 YP_908168.1 CDS MUL_4781 NC_008611.1 5295857 5296966 D cytoplasmic protein; hypothetical protein 5295857..5296966 Mycobacterium ulcerans Agy99 4550016 YP_908169.1 CDS MUL_4782 NC_008611.1 5296968 5298017 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown, probably involved in cellular metabolism.; coenzyme F420-dependent oxidoreductase complement(5296968..5298017) Mycobacterium ulcerans Agy99 4550017 YP_908170.1 CDS MUL_4783 NC_008611.1 5298017 5298541 R cytoplasmic protein; hypothetical protein complement(5298017..5298541) Mycobacterium ulcerans Agy99 4550018 YP_908171.1 CDS desA3_2 NC_008611.1 5298811 5299944 R membrane protein; thought to be involved in lipid metabolism [catalytic activity: linoleoyl-CoA + ah(2) + O(2) = gamma- linolenoyl-CoA + a + 2 H(2)O]; linoleoyl-CoA desaturase, DesA3_2 complement(5298811..5299944) Mycobacterium ulcerans Agy99 4550019 YP_908172.1 CDS MUL_4786 NC_008611.1 5299980 5301077 R cytoplasmic protein; function unknown, contains flavodoxin reductases (ferredoxin-NADPH reductases) family 1 domain. [energy production and conversion]; flavodoxin oxidoreductase complement(5299980..5301077) Mycobacterium ulcerans Agy99 4550020 YP_908173.1 CDS MUL_4787 NC_008611.1 5301148 5301816 D cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator 5301148..5301816 Mycobacterium ulcerans Agy99 4550021 YP_908174.1 CDS MUL_4789 NC_008611.1 5304070 5304618 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; acetyltransferase complement(5304070..5304618) Mycobacterium ulcerans Agy99 4550023 YP_908175.1 CDS fadE1 NC_008611.1 5304780 5306072 D Detected in the cytoplamic fraction by LC-MS/MS. Also detected in the membrane fraction by proteomics (2D- LC-MS/MS); cytoplasmic protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE1 5304780..5306072 Mycobacterium ulcerans Agy99 4550024 YP_908176.1 CDS MUL_4791 NC_008611.1 5306153 5306608 R cytoplasmic protein; acyl dehydratase complement(5306153..5306608) Mycobacterium ulcerans Agy99 4550025 YP_908177.1 CDS MUL_4792 NC_008611.1 5306645 5307142 D cytoplasmic protein; phosphoesterase 5306645..5307142 Mycobacterium ulcerans Agy99 4552146 YP_908178.1 CDS fbpC NC_008611.1 5307495 5308535 D Also detected in the cytoplasm and the membrane fractions by proteomics.; secreted protein; proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity.; secreted antigen 85-C FbpC 5307495..5308535 Mycobacterium ulcerans Agy99 4552147 YP_908179.1 CDS MUL_4796 NC_008611.1 5309411 5310778 R Detected in the extracellular matrix and the cytoplasmic fractions by proteomics.; cytoplasmic protein; hypothetical protein complement(5309411..5310778) Mycobacterium ulcerans Agy99 4552149 YP_908180.1 CDS treS NC_008611.1 5310852 5312645 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); cytoplasmic protein; involved in trehalose biosynthesis (protective effect) converts maltose to trehalose. mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)- linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway); trehalose synthase TreS complement(5310852..5312645) Mycobacterium ulcerans Agy99 4552150 YP_908181.1 CDS pepA NC_008611.1 5312758 5313789 R Three isoforms were also detected in the cytoplasm.; secreted protein; function unknown, possibly hydrolyzes peptides and/or proteins (seems to cleave preferentially after serine residues); serine protease PepA complement(5312758..5313789) Mycobacterium ulcerans Agy99 4552151 YP_908182.1 CDS MUL_4799 NC_008611.1 5313944 5314444 R cytoplasmic protein; maybe involved in oxidization of aromatic hydrocarbons; dioxygenase subunit beta complement(5313944..5314444) Mycobacterium ulcerans Agy99 4552152 YP_908183.1 CDS MUL_4800 NC_008611.1 5314506 5314916 D cytoplasmic protein; hypothetical protein 5314506..5314916 Mycobacterium ulcerans Agy99 4552153 YP_908184.1 CDS MUL_4802 NC_008611.1 5317261 5317737 D membrane protein; hypothetical protein 5317261..5317737 Mycobacterium ulcerans Agy99 4552155 YP_908185.1 CDS fadD7 NC_008611.1 5317734 5319368 R activates fatty acids by binding to coenzyme A; acyl-CoA synthetase complement(5317734..5319368) Mycobacterium ulcerans Agy99 4552156 YP_908186.1 CDS oxcA NC_008611.1 5319635 5321398 D catalyzes the formation of formyl-CoA from oxalyl-CoA; putative oxalyl-CoA decarboxylase 5319635..5321398 Mycobacterium ulcerans Agy99 4552157 YP_908187.1 CDS oxyS NC_008611.1 5321555 5322313 R cytoplasmic protein; could effect functions of OxyR during evolution.; oxidative stress response regulatory protein OxyS complement(5321555..5322313) Mycobacterium ulcerans Agy99 4552158 YP_908188.1 CDS MUL_4806 NC_008611.1 5322759 5323484 D secreted protein; hypothetical protein 5322759..5323484 Mycobacterium ulcerans Agy99 4552159 YP_908189.1 CDS MUL_4807 NC_008611.1 5323536 5325284 R membrane protein; H(+)-stimulated, highly selective, divalent cation uptake system. responsible for the translocation of the divalent metal across the membrane.; divalent cation-transport integral membrane protein complement(5323536..5325284) Mycobacterium ulcerans Agy99 4552160 YP_908190.1 CDS MUL_4808 NC_008611.1 5325392 5325574 R hypothetical protein complement(5325392..5325574) Mycobacterium ulcerans Agy99 4552161 YP_908191.1 CDS MUL_4809 NC_008611.1 5326182 5326847 D cytoplasmic protein; thought to be involved in transfer of methyl group.; O-methyltransferase 5326182..5326847 Mycobacterium ulcerans Agy99 4552162 YP_908192.1 CDS MUL_4810 NC_008611.1 5326851 5328863 R membrane protein; function unknown, probably involved in cellular metabolism.; transmembrane acyltransferase complement(5326851..5328863) Mycobacterium ulcerans Agy99 4552163 YP_908193.1 CDS MUL_4811 NC_008611.1 5329101 5330288 R cytoplasmic protein; function unknown. possibly involved in energy production and conversion.; hypothetical protein complement(5329101..5330288) Mycobacterium ulcerans Agy99 4550657 YP_908194.1 CDS MUL_4812 NC_008611.1 5330314 5331249 R cytoplasmic protein; function unknown, possibly involved in cellular metabolism.; short-chain type dehydrogenase/reductase complement(5330314..5331249) Mycobacterium ulcerans Agy99 4550658 YP_908195.1 CDS MUL_4813 NC_008611.1 5331474 5333111 R cytoplasmic protein; function unknown, probably involved in cellular metabolism; monoxygenase complement(5331474..5333111) Mycobacterium ulcerans Agy99 4550659 YP_908196.1 CDS MUL_4814 NC_008611.1 5333108 5334289 R cytoplasmic protein; function unknown, probably involved in cellular metabolism; monooxygenase complement(5333108..5334289) Mycobacterium ulcerans Agy99 4550660 YP_908197.1 CDS MUL_4817 NC_008611.1 5336713 5338335 D cytoplasmic protein; hypothetical protein 5336713..5338335 Mycobacterium ulcerans Agy99 4550663 YP_908198.1 CDS MUL_4818 NC_008611.1 5338348 5339127 D cytoplasmic protein; involved in transcriptional mechanism.; GntR family transcriptional regulator 5338348..5339127 Mycobacterium ulcerans Agy99 4550664 YP_908199.1 CDS MUL_4820 NC_008611.1 5340749 5341465 D cytoplasmic protein; function unknown, possibly involved in cellular metabolism.; short-chain type dehydrogenase/reductase 5340749..5341465 Mycobacterium ulcerans Agy99 4550666 YP_908200.1 CDS MUL_4822 NC_008611.1 5342015 5342779 R membrane protein; serine protease complement(5342015..5342779) Mycobacterium ulcerans Agy99 4550668 YP_908201.1 CDS MUL_4824 NC_008611.1 5344361 5345428 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(5344361..5345428) Mycobacterium ulcerans Agy99 4550670 YP_908202.1 CDS MUL_4825 NC_008611.1 5346190 5346396 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS, and also in the membrane fraction by proteomics (LC- MS/MS); cytoplasmic protein; hypothetical protein 5346190..5346396 Mycobacterium ulcerans Agy99 4550671 YP_908203.1 CDS ctpI NC_008611.1 5346865 5351727 D Detected in the membrane fraction by proteomics (LC- MS/MS) Also detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; cation-transporting ATPase; possibly catalyzes the transport of a cation (possibly magnesium) with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + cation(in) = ADP + phosphate + cation(out)]; cation-transporter ATPase I CtpI 5346865..5351727 Mycobacterium ulcerans Agy99 4550672 YP_908204.1 CDS fadD12_2 NC_008611.1 5351700 5353286 R activates fatty acids by binding to coenzyme A; acyl-CoA synthetase complement(5351700..5353286) Mycobacterium ulcerans Agy99 4550673 YP_908205.1 CDS MUL_4828 NC_008611.1 5353289 5354188 R cytoplasmic protein; function unknown; supposed involved in cellular metabolism.; short-chain type dehydrogenase/reductase complement(5353289..5354188) Mycobacterium ulcerans Agy99 4550674 YP_908206.1 CDS rpmE2 NC_008611.1 5354358 5354630 R RpmE2; there appears to be two types of ribosomal proteins L31 in bacterial genomes; some contain a CxxC motif while others do not; Bacillus subtilis has both types; the proteins in this cluster do not have the CXXC motif; RpmE is found in exponentially growing Bacilli while YtiA was found after exponential growth; expression of ytiA is controlled by a zinc-specific transcriptional repressor; RpmE contains one zinc ion and a CxxC motif is responsible for this binding; forms an RNP particle along with proteins L5, L18, and L25 and 5S rRNA; found crosslinked to L2 and L25 and EF-G; may be near the peptidyltransferase site of the 50S ribosome; 50S ribosomal protein L31 complement(5354358..5354630) Mycobacterium ulcerans Agy99 4550675 YP_908207.1 CDS MUL_4830 NC_008611.1 5354627 5355823 R Detected in the membrane fraction by proteomics.; cytoplasmic protein; hypothetical protein complement(5354627..5355823) Mycobacterium ulcerans Agy99 4552017 YP_908208.1 CDS rpmB2 NC_008611.1 5355953 5356189 D cytoplasmic protein; involved in ribosome activity; 50S ribosomal protein L28 5355953..5356189 Mycobacterium ulcerans Agy99 4552018 YP_908209.1 CDS rpmG NC_008611.1 5356189 5356353 D in Escherichia coli BM108, a mutation that results in lack of L33 synthesis had no effect on ribosome synthesis or function; there are paralogous genes in several bacterial genomes, and a CXXC motif for zinc binding and an upstream regulation region of the paralog lacking this motif that are regulated by zinc similar to other ribosomal proteins like L31; the proteins in this group lack the CXXC motif; 50S ribosomal protein L33 5356189..5356353 Mycobacterium ulcerans Agy99 4552019 YP_908210.1 CDS rpsN NC_008611.1 5356354 5356659 D located in the peptidyl transferase center and involved in assembly of 30S ribosome subunit; similar to what is observed with proteins L31 and L33, some proteins in this family contain CXXC motifs that are involved in zinc binding; if two copies are present in a genome, then the duplicated copy appears to have lost the zinc-binding motif and is instead regulated by zinc; the proteins in this group do not appear to have the zinc-binding motif; 30S ribosomal protein S14 5356354..5356659 Mycobacterium ulcerans Agy99 4552020 YP_908211.1 CDS rpsR2 NC_008611.1 5356666 5356929 D binds as a heterodimer with protein S6 to the central domain of the 16S rRNA; helps stabilize the platform of the 30S subunit; 30S ribosomal protein S18 5356666..5356929 Mycobacterium ulcerans Agy99 4552021 YP_908212.1 CDS MUL_4835 NC_008611.1 5356950 5357900 R cytoplasmic protein; hypothetical protein complement(5356950..5357900) Mycobacterium ulcerans Agy99 4552022 YP_908213.1 CDS MUL_4837 NC_008611.1 5358100 5358693 D cytoplasmic protein; involved in transcriptional mechanism.; transcriptional regulatory protein 5358100..5358693 Mycobacterium ulcerans Agy99 4552023 YP_908214.1 CDS lipJ NC_008611.1 5358696 5360072 D cytoplasmic protein; function unknown, probably involved in cellular metabolism; lignin peroxidase LipJ 5358696..5360072 Mycobacterium ulcerans Agy99 4552024 YP_908215.1 CDS MUL_4842 NC_008611.1 5364216 5365262 D cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 5364216..5365262 Mycobacterium ulcerans Agy99 4552028 YP_908216.1 CDS MUL_4844 NC_008611.1 5365829 5366635 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown, probably involved in cellular metabolism; short-chain type dehydrogenase/reductase 5365829..5366635 Mycobacterium ulcerans Agy99 4552030 YP_908217.1 CDS ctpB NC_008611.1 5366880 5369153 D membrane protein; cation-transporting ATPase; possibly catalyzes the transport of a cation (possibly coopper) with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + cation(in) = ADP + orthophosphate + cation(out)]; cation-transporter p-type ATPase B CtpB 5366880..5369153 Mycobacterium ulcerans Agy99 4552031 YP_908218.1 CDS MUL_4846 NC_008611.1 5369146 5369364 D Detected in the cytoplasmic and secreted protein fractions by 2D-LC-MS/MS.; secreted protein; hypothetical protein 5369146..5369364 Mycobacterium ulcerans Agy99 4552032 YP_908219.1 CDS MUL_4847 NC_008611.1 5369381 5371384 R membrane protein; integral membrane protein complement(5369381..5371384) Mycobacterium ulcerans Agy99 4552033 YP_908220.1 CDS MUL_4848 NC_008611.1 5371549 5372292 D secreted protein; hypothetical protein 5371549..5372292 Mycobacterium ulcerans Agy99 4552034 YP_908221.1 CDS sigC_1 NC_008611.1 5372328 5372882 R cytoplasmic protein; involved in promoter recognition, transcription initiation.; ECF subfamily RNA polymerase sigma factor complement(5372328..5372882) Mycobacterium ulcerans Agy99 4549613 YP_908222.1 CDS ctpA NC_008611.1 5373007 5375298 D membrane protein; cation-transporting ATPase; possibly catalyzes the transport of a cation (possibly copper) with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + cation(in) = ADP + phosphate + cation(out)]; cation transporter p-type ATPase a CtpA 5373007..5375298 Mycobacterium ulcerans Agy99 4549614 YP_908223.1 CDS MUL_4851 NC_008611.1 5375319 5376044 R membrane protein; hypothetical protein complement(5375319..5376044) Mycobacterium ulcerans Agy99 4549615 YP_908224.1 CDS omt_1 NC_008611.1 5376126 5376935 R cytoplasmic protein; function unknown, but supposed involved in lipid metabolism; O-methyltransferase Omt complement(5376126..5376935) Mycobacterium ulcerans Agy99 4549616 YP_908225.1 CDS MUL_4853 NC_008611.1 5377263 5378681 D PPE1; membrane protein; PPE family protein 5377263..5378681 Mycobacterium ulcerans Agy99 4549617 YP_908226.1 CDS MUL_4854 NC_008611.1 5378875 5379744 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 5378875..5379744 Mycobacterium ulcerans Agy99 4549618 YP_908227.1 CDS MUL_4855 NC_008611.1 5379741 5380295 D cytoplasmic protein; hypothetical protein 5379741..5380295 Mycobacterium ulcerans Agy99 4549619 YP_908228.1 CDS fadD10 NC_008611.1 5380324 5381919 D activates fatty acids by binding to coenzyme A; acyl-CoA synthetase 5380324..5381919 Mycobacterium ulcerans Agy99 4549620 YP_908229.1 CDS MUL_4857 NC_008611.1 5381924 5382163 D cytoplasmic protein; hypothetical protein 5381924..5382163 Mycobacterium ulcerans Agy99 4549621 YP_908230.1 CDS MUL_4858 NC_008611.1 5382160 5386410 D membrane protein; involved in lipid metabolism.; non-ribosomal peptide synthetase 5382160..5386410 Mycobacterium ulcerans Agy99 4549622 YP_908231.1 CDS MUL_4860 NC_008611.1 5389428 5390231 R cytoplasmic protein; function unknown, but possibly involvement in lipid metabolism.; ketoacyl reductase complement(5389428..5390231) Mycobacterium ulcerans Agy99 4549624 YP_908232.1 CDS MUL_4861 NC_008611.1 5390333 5390818 R cytoplasmic protein; hypothetical protein complement(5390333..5390818) Mycobacterium ulcerans Agy99 4549625 YP_908233.1 CDS mtn NC_008611.1 5391176 5391931 R cytoplasmic protein; responsible for cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and s- adenosylhomocysteine (sah) [catalytic activity 1: methylthioadenosine + H2O = adenine + 5-methylthio-D- ribose] [catalytic activity 2: S-adenosyl-L-homocysteine + H2O = adenine + S-D-ribosyl-L-homocysteine]; bifunctional Mta/Sah nucleosidase Mtn complement(5391176..5391931) Mycobacterium ulcerans Agy99 4549626 YP_908234.1 CDS MUL_4863 NC_008611.1 5392042 5392866 R cytoplasmic protein; hypothetical protein complement(5392042..5392866) Mycobacterium ulcerans Agy99 4549627 YP_908235.1 CDS MUL_4864 NC_008611.1 5392863 5393492 R cytoplasmic protein; possibly involved in transcriptional mechanism.; TetR family transcriptional regulator complement(5392863..5393492) Mycobacterium ulcerans Agy99 4549628 YP_908236.1 CDS MUL_4865 NC_008611.1 5393538 5394398 R cytoplasmic protein; function unknown, possibly involved in cellular metabolism.; short-chain type dehydrogenase/reductase complement(5393538..5394398) Mycobacterium ulcerans Agy99 4549629 YP_908237.1 CDS MUL_4866 NC_008611.1 5394468 5395082 D cytoplasmic protein; hypothetical protein 5394468..5395082 Mycobacterium ulcerans Agy99 4549630 YP_908238.1 CDS MUL_4867 NC_008611.1 5395107 5395877 R membrane protein; hypothetical protein complement(5395107..5395877) Mycobacterium ulcerans Agy99 4549631 YP_908239.1 CDS echA8_7 NC_008611.1 5396183 5396983 D cytoplasmic protein; oxidizes fatty acids using specific components (by similarity) [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]; enoyl-CoA hydratase, EchA8_7 5396183..5396983 Mycobacterium ulcerans Agy99 4550834 YP_908240.1 CDS MUL_4869 NC_008611.1 5397041 5397640 R cytoplasmic protein; thought to cause methylation.; methyltransferase/methylase complement(5397041..5397640) Mycobacterium ulcerans Agy99 4550835 YP_908241.1 CDS MUL_4870 NC_008611.1 5397757 5398569 D cytoplasmic protein; function unknown, possibly involved in cellular metabolism.; short chain dehydrogenase 5397757..5398569 Mycobacterium ulcerans Agy99 4550836 YP_908242.1 CDS adhD NC_008611.1 5400482 5401639 D cytoplasmic protein; function unknown, generates an aldehyde (or perhaps a ketone) from an alcohol.; zinc-type alcohol dehydrogenase AdhD 5400482..5401639 Mycobacterium ulcerans Agy99 4550838 YP_908243.1 CDS MUL_4873 NC_008611.1 5401523 5403199 R membrane protein; function unknown, involved in cellular metabolism.; monooxygenase complement(5401523..5403199) Mycobacterium ulcerans Agy99 4550839 YP_908244.1 CDS MUL_4875 NC_008611.1 5405098 5405727 R Detected in the cytoplasmic and membrane fractions by proteomics.; cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator complement(5405098..5405727) Mycobacterium ulcerans Agy99 4550841 YP_908245.1 CDS MUL_4876 NC_008611.1 5405877 5406539 D cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator 5405877..5406539 Mycobacterium ulcerans Agy99 4550842 YP_908246.1 CDS MUL_4877 NC_008611.1 5406536 5407546 D cytoplasmic protein; function unknown. possibly involved in cellular metabolism.; hypothetical protein 5406536..5407546 Mycobacterium ulcerans Agy99 4550843 YP_908247.1 CDS MUL_4879 NC_008611.1 5408172 5408645 R cytoplasmic protein; hypothetical protein complement(5408172..5408645) Mycobacterium ulcerans Agy99 4550845 YP_908248.1 CDS MUL_4881 NC_008611.1 5408848 5409060 D cytoplasmic protein; function unknown, contains copper chaperone domain.; hypothetical protein 5408848..5409060 Mycobacterium ulcerans Agy99 4550846 YP_908249.1 CDS MUL_4882 NC_008611.1 5409096 5409947 D secreted protein; hypothetical protein 5409096..5409947 Mycobacterium ulcerans Agy99 4550847 YP_908250.1 CDS MUL_4884 NC_008611.1 5412356 5413540 R membrane protein; function unknown, involved in cellular metabolism.; dioxygenase complement(5412356..5413540) Mycobacterium ulcerans Agy99 4550849 YP_908251.1 CDS MUL_4885 NC_008611.1 5413600 5414406 D cytoplasmic protein; hypothetical protein 5413600..5414406 Mycobacterium ulcerans Agy99 4550850 YP_908252.1 CDS MUL_4886 NC_008611.1 5414451 5414900 R cytoplasmic protein; function unknown. contains HTH motif. may be involved in transcription regulation.; hypothetical protein complement(5414451..5414900) Mycobacterium ulcerans Agy99 4550851 YP_908253.1 CDS MUL_4888 NC_008611.1 5415969 5416559 R cytoplasmic protein; hypothetical protein complement(5415969..5416559) Mycobacterium ulcerans Agy99 4554172 YP_908254.1 CDS MUL_4889 NC_008611.1 5416573 5417196 R secreted protein; hypothetical protein complement(5416573..5417196) Mycobacterium ulcerans Agy99 4554173 YP_908255.1 CDS MUL_4890 NC_008611.1 5417335 5417940 R cytoplasmic protein; possibly involved in transcriptional mechanism.; transcriptional regulatory protein complement(5417335..5417940) Mycobacterium ulcerans Agy99 4554174 YP_908256.1 CDS MUL_4891 NC_008611.1 5418001 5418831 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 5418001..5418831 Mycobacterium ulcerans Agy99 4554175 YP_908257.1 CDS MUL_4892 NC_008611.1 5418895 5419488 R cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator complement(5418895..5419488) Mycobacterium ulcerans Agy99 4554176 YP_908258.1 CDS MUL_4893 NC_008611.1 5419623 5420648 D cytoplasmic protein; hypothetical protein 5419623..5420648 Mycobacterium ulcerans Agy99 4554177 YP_908259.1 CDS MUL_4895 NC_008611.1 5421294 5422637 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(5421294..5422637) Mycobacterium ulcerans Agy99 4554179 YP_908260.1 CDS MUL_4896 NC_008611.1 5423065 5424111 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(5423065..5424111) Mycobacterium ulcerans Agy99 4554180 YP_908261.1 CDS MUL_4899 NC_008611.1 5426406 5426963 D cytoplasmic protein; hypothetical protein 5426406..5426963 Mycobacterium ulcerans Agy99 4554183 YP_908262.1 CDS MUL_4900 NC_008611.1 5427022 5428635 R membrane protein; function unknown, probably involved in cellular metabolism; hypothetical protein complement(5427022..5428635) Mycobacterium ulcerans Agy99 4554184 YP_908263.1 CDS sdaA NC_008611.1 5428753 5430138 R cytoplasmic protein; involved in gluconeogenesis from serine [catalytic activity: L-serine + H2O = pyruvate + NH3 + H2O]; L-serine dehydratase SdaA complement(5428753..5430138) Mycobacterium ulcerans Agy99 4554185 YP_908264.1 CDS glyA NC_008611.1 5430135 5431412 R catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate; serine hydroxymethyltransferase complement(5430135..5431412) Mycobacterium ulcerans Agy99 4554186 YP_908265.1 CDS gcvH_1 NC_008611.1 5431409 5431813 R cytoplasmic protein; the glycine cleavage system catalyses the degradation of glycine. the H protein shuttles the methylamine group of glycine from the P protein to the T protein; glycine cleavage system H protein GcvH complement(5431409..5431813) Mycobacterium ulcerans Agy99 4554187 YP_908266.1 CDS MUL_4906 NC_008611.1 5436233 5436331 D hypothetical protein 5436233..5436331 Mycobacterium ulcerans Agy99 4553749 YP_908267.1 CDS MUL_4908 NC_008611.1 5437540 5438586 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(5437540..5438586) Mycobacterium ulcerans Agy99 4553751 YP_908268.1 CDS MUL_4909 NC_008611.1 5439094 5440269 R membrane protein; PE-PGRS family protein complement(5439094..5440269) Mycobacterium ulcerans Agy99 4553752 YP_908269.1 CDS esxP_2 NC_008611.1 5440660 5440929 R disruption by insertion sequence (missing 11 aa); secreted protein; EsaT-6 like protein EsxP_2 complement(5440660..5440929) Mycobacterium ulcerans Agy99 4553753 YP_908270.1 CDS MUL_4911 NC_008611.1 5441233 5441937 D cytoplasmic protein; required for the transposition of insertion element IS2404.; N-term transposase for IS2404 5441233..5441937 Mycobacterium ulcerans Agy99 4553754 YP_908271.1 CDS MUL_4912 NC_008611.1 5441962 5442279 D cytoplasmic protein; required for the transposition of insertion element IS2404.; C-term transposase for IS2404 5441962..5442279 Mycobacterium ulcerans Agy99 4553755 YP_908272.1 CDS mce6F NC_008611.1 5444883 5446121 R membrane protein; function unknown, but thought involved in host cell invasion.; MCE-family protein Mce6F complement(5444883..5446121) Mycobacterium ulcerans Agy99 4553757 YP_908273.1 CDS mce6E NC_008611.1 5446118 5447245 R membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE family lipoprotein Mce6E complement(5446118..5447245) Mycobacterium ulcerans Agy99 4553758 YP_908274.1 CDS mce6D NC_008611.1 5447242 5448354 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE-family protein Mce6D complement(5447242..5448354) Mycobacterium ulcerans Agy99 4553759 YP_908275.1 CDS mce6C NC_008611.1 5448351 5449397 R membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE-family protein Mce6C complement(5448351..5449397) Mycobacterium ulcerans Agy99 4553760 YP_908276.1 CDS mce6B NC_008611.1 5449390 5450418 R membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE-family protein Mce6B complement(5449390..5450418) Mycobacterium ulcerans Agy99 4553761 YP_908277.1 CDS mce6A NC_008611.1 5450482 5451972 R membrane protein; function unknown, but thought to be involved in host cell invasion.; MCE-family protein Mce6A complement(5450482..5451972) Mycobacterium ulcerans Agy99 4553762 YP_908278.1 CDS yrbE6B NC_008611.1 5451975 5452841 R membrane protein; integral membrane protein YrbE6B complement(5451975..5452841) Mycobacterium ulcerans Agy99 4553763 YP_908279.1 CDS yrbE6A NC_008611.1 5452843 5453688 R membrane protein; integral membrane protein YrbE6A complement(5452843..5453688) Mycobacterium ulcerans Agy99 4553764 YP_908280.1 CDS pks16_1 NC_008611.1 5453698 5455350 R activates fatty acids by binding to coenzyme A; long-chain-fatty-acid--CoA ligase complement(5453698..5455350) Mycobacterium ulcerans Agy99 4551469 YP_908281.1 CDS MUL_4923 NC_008611.1 5455347 5455628 R Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; thought to be involved in de novo fatty acid biosynthesis; this protein is the carrier of the growing fatty acid chain in fatty acid biosynthesis; acyl carrier protein complement(5455347..5455628) Mycobacterium ulcerans Agy99 4551470 YP_908282.1 CDS MUL_4924 NC_008611.1 5455644 5456420 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); membrane protein; hypothetical protein complement(5455644..5456420) Mycobacterium ulcerans Agy99 4551471 YP_908283.1 CDS MUL_4925 NC_008611.1 5456417 5457391 R Detected in the membrane fraction by proteomics (LC- MS/MS) Also detected in the cytoplamic and extracellular matrix fractions.; membrane protein; probably involved in active transport of substrate across the membrane (export) responsible for energy coupling to the transport system.; ABC transporter ATP-binding protein complement(5456417..5457391) Mycobacterium ulcerans Agy99 4551472 YP_908284.1 CDS MUL_4926 NC_008611.1 5457366 5458094 R Also detected in the cytoplamic, the extracellular matrix and the membrane fractions by proteomics.; membrane protein; hypothetical protein complement(5457366..5458094) Mycobacterium ulcerans Agy99 4551473 YP_908285.1 CDS MUL_4927 NC_008611.1 5458106 5458612 R Detected in cytoplasmic and membrane fraction by proteomics.; cytoplasmic protein; hypothetical protein complement(5458106..5458612) Mycobacterium ulcerans Agy99 4551474 YP_908286.1 CDS MUL_4928 NC_008611.1 5458609 5459352 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); cytoplasmic protein; hypothetical protein complement(5458609..5459352) Mycobacterium ulcerans Agy99 4551475 YP_908287.1 CDS MUL_4929 NC_008611.1 5459349 5460857 R Detected in the cytoplamic fraction by LC-MS/MS. Also detected in the extracellular matrix and membrane fraction by proteomics.; cytoplasmic protein; catalyzes the production of GabA [catalytic activity: L-glutamate = 4-aminobutanoate + CO(2)]; glutamate decarboxylase complement(5459349..5460857) Mycobacterium ulcerans Agy99 4551476 YP_908288.1 CDS gabT_2 NC_008611.1 5460854 5462203 R Detected in the membrane fraction by proteomics.; cytoplasmic protein; 4-aminobutyrate aminotransferase complement(5460854..5462203) Mycobacterium ulcerans Agy99 4551477 YP_908289.1 CDS MUL_4931 NC_008611.1 5462203 5463300 R membrane protein; function unknown.; hypothetical protein complement(5462203..5463300) Mycobacterium ulcerans Agy99 4551478 YP_908290.1 CDS MUL_4932 NC_008611.1 5463297 5464277 R Detected in the membrane fraction by proteomics.; membrane protein; function unknown, probably involved in cellular metabolism.; nucleoside-diphosphate-sugar epimerase complement(5463297..5464277) Mycobacterium ulcerans Agy99 4551479 YP_908291.1 CDS MUL_4933 NC_008611.1 5464277 5465458 R Also detected in the membrane fraction by proteomics.; cytoplasmic protein; hypothetical protein complement(5464277..5465458) Mycobacterium ulcerans Agy99 4551480 YP_908292.1 CDS MUL_4934 NC_008611.1 5465451 5466539 R membrane protein; hypothetical protein complement(5465451..5466539) Mycobacterium ulcerans Agy99 4551481 YP_908293.1 CDS MUL_4935 NC_008611.1 5466914 5467639 D membrane protein; hypothetical protein 5466914..5467639 Mycobacterium ulcerans Agy99 4551482 YP_908294.1 CDS MUL_4936 NC_008611.1 5467684 5468442 D secreted protein; function unknown. possibly secreted.; hypothetical protein 5467684..5468442 Mycobacterium ulcerans Agy99 4551483 YP_908295.1 CDS MUL_4938 NC_008611.1 5468945 5469268 D membrane protein; hypothetical protein 5468945..5469268 Mycobacterium ulcerans Agy99 4551484 YP_908296.1 CDS icd2 NC_008611.1 5469945 5472182 D Also detected in the membrane fraction by proteomics (LC-MS/MS); cytoplasmic protein; involved in the krebs cycle [catalytic activity: isocitrate + NADP+ = 2-oxoglutarate + CO(2) + NADPH]; isocitrate dehydrogenase [NADP] Icd2 5469945..5472182 Mycobacterium ulcerans Agy99 4551485 YP_908297.1 CDS cyaA NC_008611.1 5472296 5473420 D cytoplasmic protein; possibly involved in camp synthesis [catalytic activity: ATP = 3',5'-cyclic AMP + diphosphate]; adenylate cyclase CyaA 5472296..5473420 Mycobacterium ulcerans Agy99 4551486 YP_908298.1 CDS fadE36_1 NC_008611.1 5474796 5475851 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS.; cytoplasmic protein; function unknown, but possibly involvement in lipid degradation.; acyl-CoA dehydrogenase FadE36 5474796..5475851 Mycobacterium ulcerans Agy99 4551488 YP_908299.1 CDS MUL_4943 NC_008611.1 5475873 5476913 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; coenzyme F420-dependent oxidoreductase complement(5475873..5476913) Mycobacterium ulcerans Agy99 4552266 YP_908300.1 CDS MUL_4944 NC_008611.1 5476972 5477403 R cytoplasmic protein; hypothetical protein complement(5476972..5477403) Mycobacterium ulcerans Agy99 4552267 YP_908301.1 CDS MUL_4945 NC_008611.1 5477531 5478097 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 5477531..5478097 Mycobacterium ulcerans Agy99 4552268 YP_908302.1 CDS MUL_4947 NC_008611.1 5479535 5480179 D cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator 5479535..5480179 Mycobacterium ulcerans Agy99 4552270 YP_908303.1 CDS fadE25_2 NC_008611.1 5480184 5481395 D Detected in the cytoplasmic fraction by proteomics. Also detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown, but involved in lipid degradation [catalytic activity: acyl-CoA + ETF = 2,3-dehydroacyl-CoA + reduced ETF]; acyl-CoA dehydrogenase FadE25_2 5480184..5481395 Mycobacterium ulcerans Agy99 4552271 YP_908304.1 CDS MUL_4949 NC_008611.1 5481464 5482672 D membrane protein; function unknown, glycosyltransferases, related to UDP-glucuronosyltransferase; glycosyltransferase 5481464..5482672 Mycobacterium ulcerans Agy99 4552272 YP_908305.1 CDS MUL_4950 NC_008611.1 5482849 5483469 D secreted protein; function unknown.; hypothetical protein 5482849..5483469 Mycobacterium ulcerans Agy99 4552273 YP_908306.1 CDS MUL_4951 NC_008611.1 5483491 5484654 R cytoplasmic protein; ATPase complement(5483491..5484654) Mycobacterium ulcerans Agy99 4552274 YP_908307.1 CDS MUL_4952 NC_008611.1 5484788 5485279 D cytoplasmic protein; transcriptional regulator 5484788..5485279 Mycobacterium ulcerans Agy99 4552275 YP_908308.1 CDS MUL_4953 NC_008611.1 5485310 5485726 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 5485310..5485726 Mycobacterium ulcerans Agy99 4552276 YP_908309.1 CDS MUL_4954 NC_008611.1 5485822 5486220 D cytoplasmic protein; hypothetical protein 5485822..5486220 Mycobacterium ulcerans Agy99 4552277 YP_908310.1 CDS ltp1_1 NC_008611.1 5486251 5487462 D membrane protein; possibly catalyzes the transfer of a great variety of lipids between membranes.; lipid-transfer protein Ltp1 5486251..5487462 Mycobacterium ulcerans Agy99 4552278 YP_908311.1 CDS MUL_4956 NC_008611.1 5487471 5488046 R Detected in the membrane fraction by proteomics (2D- LC-MS/MS); cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator complement(5487471..5488046) Mycobacterium ulcerans Agy99 4552279 YP_908312.1 CDS MUL_4957 NC_008611.1 5488361 5489227 D cytoplasmic protein; hypothetical protein 5488361..5489227 Mycobacterium ulcerans Agy99 4552280 YP_908313.1 CDS fadE6_1 NC_008611.1 5489235 5491460 D membrane protein; function unknown, but involved in lipid degradation.; acyl-CoA dehydrogenase FadE6 5489235..5491460 Mycobacterium ulcerans Agy99 4552281 YP_908314.1 CDS MUL_4959 NC_008611.1 5491616 5492809 D cytoplasmic protein; hypothetical protein 5491616..5492809 Mycobacterium ulcerans Agy99 4552282 YP_908315.1 CDS MUL_4960 NC_008611.1 5492849 5493115 R cytoplasmic protein; hypothetical protein complement(5492849..5493115) Mycobacterium ulcerans Agy99 4552283 YP_908316.1 CDS MUL_4961 NC_008611.1 5493167 5494351 R membrane protein; intergral membrane protein complement(5493167..5494351) Mycobacterium ulcerans Agy99 4552284 YP_908317.1 CDS MUL_4962 NC_008611.1 5494510 5495229 R cytoplasmic protein; hypothetical protein complement(5494510..5495229) Mycobacterium ulcerans Agy99 4551037 YP_908318.1 CDS MUL_4963 NC_008611.1 5495239 5495598 R cytoplasmic protein; hypothetical protein complement(5495239..5495598) Mycobacterium ulcerans Agy99 4551038 YP_908319.1 CDS MUL_4964 NC_008611.1 5496012 5497058 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(5496012..5497058) Mycobacterium ulcerans Agy99 4551039 YP_908320.1 CDS MUL_4967 NC_008611.1 5499699 5500184 D Regulates the synthesis of nucleoside triphosphates for nucleic acid synthesis, CTP for lipid synthesis, and GTP for protein elongation; nucleoside diphosphate kinase regulator 5499699..5500184 Mycobacterium ulcerans Agy99 4551043 YP_908321.1 CDS MUL_4968 NC_008611.1 5500244 5500684 D membrane protein; hypothetical protein 5500244..5500684 Mycobacterium ulcerans Agy99 4551044 YP_908322.1 CDS MUL_4969 NC_008611.1 5500784 5502175 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown, probably involved in cellular metabolism.; oxidoreductase 5500784..5502175 Mycobacterium ulcerans Agy99 4551045 YP_908323.1 CDS MUL_4970 NC_008611.1 5502176 5502940 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown, supposed involved in cellular metabolism.; short chain dehydrogenase 5502176..5502940 Mycobacterium ulcerans Agy99 4551046 YP_908324.1 CDS MUL_4971 NC_008611.1 5502943 5504907 D membrane protein; hypothetical protein 5502943..5504907 Mycobacterium ulcerans Agy99 4551047 YP_908325.1 CDS embC NC_008611.1 5504907 5508179 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in the biosynthesis of the mycobacterial cell wall arabinan and resistance to ethambutol (Emb; dextro-2,2'-(ethylenediimino)-di-1-butanol) polymerizes arabinose into the arabinan of arabiogalactan [catalytic activity: UDP-L-arabinose + indol-3-ylacetyl-myo-inositol = UDP + indol-3-ylacetyl-myo-inositol L-arabinoside]; integral membrane indolylacetylinositol arabinosyltransferase EmbC 5504907..5508179 Mycobacterium ulcerans Agy99 4551048 YP_908326.1 CDS embA NC_008611.1 5508389 5511730 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in the biosynthesis of the mycobacterial cell wall arabinan and resistance to ethambutol (Emb; dextro-2,2'-(ethylenediimino)-di-1-butanol) polymerizes arabinose into the arabinan of arabiogalactan [catalytic activity: UDP-L-arabinose + indol-3-ylacetyl-myo-inositol = UDP + indol-3-ylacetyl-myo-inositol L-arabinoside]; integral membrane indolylacetylinositol arabinosyltransferase EmbA 5508389..5511730 Mycobacterium ulcerans Agy99 4551049 YP_908327.1 CDS embB NC_008611.1 5511727 5514954 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; involved in the biosynthesis of the mycobacterial cell wall arabinan and resistance to ethambutol (Emb; dextro-2,2'-(ethylenediimino)-di-1-butanol) polymerizes arabinose into the arabinan of arabiogalactan [catalytic activity: UDP-L-arabinose + indol-3-ylacetyl-myo-inositol = UDP + indol-3-ylacetyl-myo-inositol L-arabinoside]; integral membrane indolylacetylinositol arabinosyltransferase EmbB 5511727..5514954 Mycobacterium ulcerans Agy99 4551050 YP_908328.1 CDS MUL_4975 NC_008611.1 5515263 5516309 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(5515263..5516309) Mycobacterium ulcerans Agy99 4551051 YP_908329.1 CDS MUL_4978 NC_008611.1 5517894 5518232 D secreted protein; hypothetical protein 5517894..5518232 Mycobacterium ulcerans Agy99 4551053 YP_908330.1 CDS MUL_4979 NC_008611.1 5518375 5518842 D membrane protein; hypothetical protein 5518375..5518842 Mycobacterium ulcerans Agy99 4551054 YP_908331.1 CDS accD4_1 NC_008611.1 5523988 5525538 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; key enzyme in the catabolic pathway of odd-chain fatty acids, isoleucine, threonine, methionine, and valine [catalytic activity: ATP + propionyl-CoA + CO(2) + H(2)O = ADP + orthophosphate + methylmalonyl-CoA]; propionyl-CoA carboxylase beta chain 4 AccD4 complement(5523988..5525538) Mycobacterium ulcerans Agy99 4552625 YP_908332.1 CDS pks13 NC_008611.1 5525568 5530919 R cytoplasmic protein; the enzyme responsible for the final condensation step in mycolic acid biosynthesis, bringing the short and long (mero) chains together.; polyketide synthase Pks13 complement(5525568..5530919) Mycobacterium ulcerans Agy99 4552626 YP_908333.1 CDS fadD32 NC_008611.1 5530942 5532849 R activates fatty acids by binding to coenzyme A; long-chain-fatty-acid--CoA ligase complement(5530942..5532849) Mycobacterium ulcerans Agy99 4552627 YP_908334.1 CDS MUL_4985 NC_008611.1 5533148 5534161 R Detected in the membrane fraction by proteomics (LC- MS/MS); secreted protein; hypothetical protein complement(5533148..5534161) Mycobacterium ulcerans Agy99 4552628 YP_908335.1 CDS fbpD NC_008611.1 5534398 5535315 R Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); secreted protein; involved in cell wall mycoloylation. proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity.; secreted Mpt51/Mpb51 antigen protein FbpD complement(5534398..5535315) Mycobacterium ulcerans Agy99 4552629 YP_908336.1 CDS fbpA NC_008611.1 5535479 5536492 R Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); secreted protein; involved in cell wall mycoloylation. proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity.; secreted antigen 85-A FbpA complement(5535479..5536492) Mycobacterium ulcerans Agy99 4552630 YP_908337.1 CDS MUL_4989 NC_008611.1 5536909 5538828 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; transmembrane protein complement(5536909..5538828) Mycobacterium ulcerans Agy99 4552631 YP_908338.1 CDS MUL_4990 NC_008611.1 5538875 5539783 R catalyzes the formation of decaprenylphosphoryl-5-phosphoribose from phosphoribose diphosphate and decaprenyl phosphate; phosphoribose diphosphate:decaprenyl-phosphate phosphoribosyltransferase complement(5538875..5539783) Mycobacterium ulcerans Agy99 4552632 YP_908339.1 CDS MUL_4991 NC_008611.1 5539780 5540325 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; transmembrane protein complement(5539780..5540325) Mycobacterium ulcerans Agy99 4552633 YP_908340.1 CDS glfT NC_008611.1 5540318 5542216 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; converts UDP-galactofuranose in cell wall galactan polymerization. has UDP-GalF:beta-D-(1->5) and UDP- GalF:beta-D-(1->6) galactofuranosyltransferase activities.; bifunctional UDP-galactofuranosyl transferase GlfT complement(5540318..5542216) Mycobacterium ulcerans Agy99 4552634 YP_908341.1 CDS glf NC_008611.1 5542213 5543421 R cytoplasmic protein; involved in lipopolysaccharide biosynthesis, in the conversion of UDP-galactopyranose into UDP-galactofuranose through a 2-keto intermediate [catalytic activity: UDP-D- galactopyranose = UDP-D-galacto-1,4-furanose]; UDP-galactopyranose mutase Glf complement(5542213..5543421) Mycobacterium ulcerans Agy99 4552635 YP_908342.1 CDS erp NC_008611.1 5543710 5544636 D secreted protein; surface-exposed protein required for multiplication and intracellular growth. seems to play a role in virulence.; exported repetitive protein Erp 5543710..5544636 Mycobacterium ulcerans Agy99 4552636 YP_908343.1 CDS MUL_4995 NC_008611.1 5544837 5546462 D secreted protein; hypothetical protein 5544837..5546462 Mycobacterium ulcerans Agy99 4552637 YP_908344.1 CDS MUL_4996 NC_008611.1 5546478 5547251 R cytoplasmic protein; hypothetical protein complement(5546478..5547251) Mycobacterium ulcerans Agy99 4552638 YP_908345.1 CDS MUL_4997 NC_008611.1 5547302 5548087 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; acyltransferase complement(5547302..5548087) Mycobacterium ulcerans Agy99 4552394 YP_908346.1 CDS MUL_4998 NC_008611.1 5548121 5548876 R cytoplasmic protein; function unknown, probably involved in cellular metabolism.; acyltransferase complement(5548121..5548876) Mycobacterium ulcerans Agy99 4552395 YP_908347.1 CDS MUL_4999 NC_008611.1 5548880 5549659 R Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; function unknown, probably involved in cellular metabolism.; acyltransferase complement(5548880..5549659) Mycobacterium ulcerans Agy99 4552396 YP_908348.1 CDS MUL_5000 NC_008611.1 5549732 5550562 D cytoplasmic protein; function unknown, probably involved in cellular metabolism [catalytic activity: ATP + substrate = ADP + substrate 3'-phosphate]; phosphotransferase 5549732..5550562 Mycobacterium ulcerans Agy99 4552397 YP_908349.1 CDS MUL_5001 NC_008611.1 5550526 5552076 D cytoplasmic protein; hypothetical protein 5550526..5552076 Mycobacterium ulcerans Agy99 4552398 YP_908350.1 CDS MUL_5002 NC_008611.1 5552073 5552405 D cytoplasmic protein; hypothetical protein 5552073..5552405 Mycobacterium ulcerans Agy99 4552399 YP_908351.1 CDS MUL_5004 NC_008611.1 5554005 5554652 R cytoplasmic protein; involved in transcriptional mechanism.; TetR family transcriptional regulator complement(5554005..5554652) Mycobacterium ulcerans Agy99 4552401 YP_908352.1 CDS MUL_5005 NC_008611.1 5554734 5555216 D membrane protein; hypothetical protein 5554734..5555216 Mycobacterium ulcerans Agy99 4552402 YP_908353.1 CDS serS NC_008611.1 5555213 5556472 R catalyzes a two-step reaction, first charging a serine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; seryl-tRNA synthetase complement(5555213..5556472) Mycobacterium ulcerans Agy99 4552403 YP_908354.1 CDS MUL_5007 NC_008611.1 5556631 5558007 D membrane protein; hypothetical protein 5556631..5558007 Mycobacterium ulcerans Agy99 4552404 YP_908355.1 CDS MUL_5008 NC_008611.1 5558012 5558362 D cytoplasmic protein; hypothetical protein 5558012..5558362 Mycobacterium ulcerans Agy99 4552405 YP_908356.1 CDS MUL_5010 NC_008611.1 5558642 5559328 R cytoplasmic protein; thought to be involved in glycolisis and perhaps glycogen metabolism [catalytic activity: 3- phosphoglycerate = 2-phosphoglycerate]; phosphoglycerate mutase complement(5558642..5559328) Mycobacterium ulcerans Agy99 4552406 YP_908357.1 CDS pheA NC_008611.1 5559325 5560272 R catalyzes the formation of phenylpyruvate from prephenate in phenylalanine biosynthesis; prephenate dehydratase complement(5559325..5560272) Mycobacterium ulcerans Agy99 4552407 YP_908358.1 CDS MUL_5012 NC_008611.1 5560368 5561168 D cytoplasmic protein; hypothetical protein 5560368..5561168 Mycobacterium ulcerans Agy99 4552408 YP_908359.1 CDS MUL_5013 NC_008611.1 5561249 5562478 D membrane protein; supposed involved in transcriptional mechanism.; transcriptional regulatory protein 5561249..5562478 Mycobacterium ulcerans Agy99 4552409 YP_908360.1 CDS bfrB NC_008611.1 5562681 5563226 D Also detected in the extracellular matrix and the membrane fraction by proteomics.; cytoplasmic protein; involved in iron storage; ferritin is an intracellular molecule that stores iron in a soluble, nontoxic, readily available form. the functional molecule, which is composed of 24 chains, is roughly spherical and contains a central cavity in which the polymeric ferric iron core is deposited.; bacterioferritin BfrB 5562681..5563226 Mycobacterium ulcerans Agy99 4552410 YP_908361.1 CDS glpQ1 NC_008611.1 5563223 5564068 R cytoplasmic protein; glycerophosphoryl diester phosphodiesterase hydrolyzes deacylated phospholipids to G3P and the corresponding alcohols [catalytic activity: a glycerophosphodiester + H(2)O = an alcohol + SN-glycerol 3- phosphate]; glycerophosphoryl diester phosphodiesterase complement(5563223..5564068) Mycobacterium ulcerans Agy99 4552411 YP_908362.1 CDS MUL_5016 NC_008611.1 5564074 5565147 R membrane protein; hypothetical protein complement(5564074..5565147) Mycobacterium ulcerans Agy99 4552412 YP_908363.1 CDS MUL_5017 NC_008611.1 5565614 5565769 D hypothetical protein 5565614..5565769 Mycobacterium ulcerans Agy99 4552413 YP_908364.1 CDS sodA NC_008611.1 5565918 5566541 D Three isoforms were detected in the cytoplasm. Also detected in the extracellular matrix and the membrane fraction by proteomics.; cytoplasmic protein; destroys radicals which are normally produced within the cells and are toxic to biological systems [catalytic activity: 2 peroxide radical + 2 H(+) = O(2) + H(2)O(2)]; superoxide dismutase [Fe] SodA 5565918..5566541 Mycobacterium ulcerans Agy99 4549650 YP_908365.1 CDS MUL_5019 NC_008611.1 5566875 5567291 D membrane protein; hypothetical protein 5566875..5567291 Mycobacterium ulcerans Agy99 4549651 YP_908366.1 CDS MUL_5020 NC_008611.1 5567544 5568248 D membrane protein; hypothetical protein 5567544..5568248 Mycobacterium ulcerans Agy99 4549652 YP_908367.1 CDS MUL_5021 NC_008611.1 5568711 5569109 D Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); cytoplasmic protein; hypothetical protein 5568711..5569109 Mycobacterium ulcerans Agy99 4549653 YP_908368.1 CDS MUL_5022 NC_008611.1 5569234 5569890 D Detected in the membrane fraction by proteomics (LC- MS/MS); membrane protein; hypothetical protein 5569234..5569890 Mycobacterium ulcerans Agy99 4549654 YP_908369.1 CDS MUL_5023 NC_008611.1 5569963 5570214 D secreted protein; hypothetical protein 5569963..5570214 Mycobacterium ulcerans Agy99 4549655 YP_908370.1 CDS hns NC_008611.1 5570330 5570809 D cytoplasmic protein; histone-like protein Hns 5570330..5570809 Mycobacterium ulcerans Agy99 4549656 YP_908371.1 CDS menG NC_008611.1 5570813 5571310 D regulator of RNase E; increases half-life and abundance of RNAs; interacts with RNase E possibly inhibiting catalytic activity; ribonuclease activity regulator protein RraA 5570813..5571310 Mycobacterium ulcerans Agy99 4549657 YP_908372.1 CDS ethA NC_008611.1 5571318 5572787 R cytoplasmic protein; activates the pro-drug ethionamide (eth); induced eth sensitivity when overexpressed in mycobacterium tuberculosis.; monooxygenase EthA complement(5571318..5572787) Mycobacterium ulcerans Agy99 4549658 YP_908373.1 CDS ethR NC_008611.1 5572863 5573510 D cytoplasmic protein; regulates negatively the production of EthA. induced eth resistance when overexpressed in mycobacterium tuberculosis.; TetR family transcriptional regulator 5572863..5573510 Mycobacterium ulcerans Agy99 4549659 YP_908374.1 CDS dinP NC_008611.1 5573452 5574741 D involved in translesion DNA polymerization with beta clamp of polymerase III; belongs to Y family of polymerases; does not contain proofreading function; DNA polymerase IV 5573452..5574741 Mycobacterium ulcerans Agy99 4549660 YP_908375.1 CDS MUL_5029 NC_008611.1 5574730 5575737 R cytoplasmic protein; hypothetical protein complement(5574730..5575737) Mycobacterium ulcerans Agy99 4549661 YP_908376.1 CDS MUL_5031 NC_008611.1 5576379 5576630 D secreted protein; hypothetical protein 5576379..5576630 Mycobacterium ulcerans Agy99 4549663 YP_908377.1 CDS mpt64 NC_008611.1 5576834 5577526 D Also detected in the cytoplasm by proteomics.; secreted protein; immunogenic protein Mpt64 5576834..5577526 Mycobacterium ulcerans Agy99 4549664 YP_908378.1 CDS MUL_5033 NC_008611.1 5577540 5578460 R cytoplasmic protein; hypothetical protein complement(5577540..5578460) Mycobacterium ulcerans Agy99 4549665 YP_908379.1 CDS MUL_5034 NC_008611.1 5578724 5579575 D cytoplasmic protein; hypothetical protein 5578724..5579575 Mycobacterium ulcerans Agy99 4549666 YP_908380.1 CDS MUL_5035 NC_008611.1 5579649 5579759 R hypothetical protein complement(5579649..5579759) Mycobacterium ulcerans Agy99 4549667 YP_908381.1 CDS gltD NC_008611.1 5579943 5581409 R glutamate synthase is composed of subunits alpha and beta; beta subunit is a flavin adenine dinucleotide-NADPH dependent oxidoreductase; provides electrons to the alpha subunit, which binds L-glutamine and 2-oxoglutarate and forms L-glutamate; glutamate synthase subunit beta complement(5579943..5581409) Mycobacterium ulcerans Agy99 4549668 YP_908382.1 CDS gltB NC_008611.1 5581402 5585985 R cytoplasmic protein; probably involved in glutamate biosynthesis [catalytic activity: 2 L-glutamate + NADP(+) = L-glutamine + 2-oxoglutarate + NADPH]; ferredoxin-dependent glutamate synthase large subunit GltB complement(5581402..5585985) Mycobacterium ulcerans Agy99 4553624 YP_908383.1 CDS MUL_5039 NC_008611.1 5587407 5588453 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(5587407..5588453) Mycobacterium ulcerans Agy99 4553627 YP_908384.1 CDS MUL_5040 NC_008611.1 5588813 5590147 R cytoplasmic protein; required for the transposition of the insertion element IS2606.; transposase for IS2606 complement(5588813..5590147) Mycobacterium ulcerans Agy99 4553628 YP_908385.1 CDS MUL_5045 NC_008611.1 5598784 5599680 R membrane protein; hypothetical protein complement(5598784..5599680) Mycobacterium ulcerans Agy99 4553633 YP_908386.1 CDS MUL_5046 NC_008611.1 5599703 5600749 R cytoplasmic protein; required for the transposition of insertion element IS2404.; transposase for IS2404 complement(5599703..5600749) Mycobacterium ulcerans Agy99 4553634 YP_908387.1 CDS MUL_5048 NC_008611.1 5601360 5602235 R cytoplasmic protein; hypothetical protein complement(5601360..5602235) Mycobacterium ulcerans Agy99 4553636 YP_908388.1 CDS MUL_5049 NC_008611.1 5602769 5602996 D cytoplasmic protein; hypothetical protein 5602769..5602996 Mycobacterium ulcerans Agy99 4553637 YP_908389.1 CDS esxE NC_008611.1 5606957 5607229 R membrane protein; EsaT-6 like protein EsxE complement(5606957..5607229) Mycobacterium ulcerans Agy99 4553135 YP_908390.1 CDS esxF NC_008611.1 5607153 5607551 R membrane protein; EsaT-6 like protein EsxF complement(5607153..5607551) Mycobacterium ulcerans Agy99 4553136 YP_908391.1 CDS lppJ NC_008611.1 5607662 5608150 R membrane protein; lipoprotein LppJ complement(5607662..5608150) Mycobacterium ulcerans Agy99 4553137 YP_908392.1 CDS MUL_5057 NC_008611.1 5608230 5610185 R cytoplasmic protein; hypothetical protein complement(5608230..5610185) Mycobacterium ulcerans Agy99 4553138 YP_908393.1 CDS MUL_5058 NC_008611.1 5610614 5610928 R cytoplasmic protein; hypothetical protein complement(5610614..5610928) Mycobacterium ulcerans Agy99 4553139 YP_908394.1 CDS pcnA NC_008611.1 5611594 5613039 R cytoplasmic protein; involved in transcription mechanism [catalytic activity: N ATP + (nucleotide)(M) = N diphosphate + (nucleotide)(M+N)]; poly(a) polymerase PcnA complement(5611594..5613039) Mycobacterium ulcerans Agy99 4553141 YP_908395.1 CDS MUL_5061 NC_008611.1 5613205 5613951 D cytoplasmic protein; hypothetical protein 5613205..5613951 Mycobacterium ulcerans Agy99 4553142 YP_908396.1 CDS MUL_5062 NC_008611.1 5613978 5616329 D secreted protein; hypothetical protein 5613978..5616329 Mycobacterium ulcerans Agy99 4553143 YP_908397.1 CDS MUL_5063 NC_008611.1 5616446 5619988 D Detected in the membrane fraction by proteomics (LC- MS/MS) Also detected in the cytoplasmic fraction by 2D-LC- MS/MS.; membrane protein; transmembrane protein 5616446..5619988 Mycobacterium ulcerans Agy99 4553144 YP_908398.1 CDS sigM NC_008611.1 5620050 5620637 D Member of the extracytoplasmic function sigma factors which are active under specific conditions; binds with the catalytic core of RNA polymerase to produce the holoenzyme and directs bacterial core RNA polymerase to specific promoter elements to initiate transcription: in Mycobacterium bovis this protein has been shown to be active at high temperatures and during stationary phase; RNA polymerase sigma factor SigM 5620050..5620637 Mycobacterium ulcerans Agy99 4553145 YP_908399.1 CDS MUL_5065 NC_008611.1 5620713 5621462 D membrane protein; hypothetical protein 5620713..5621462 Mycobacterium ulcerans Agy99 4553146 YP_908400.1 CDS trxB2 NC_008611.1 5621552 5622550 D Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the extracellular matrix and membrane fraction by proteomics.; cytoplasmic protein; enzyme that catalyse the reduction of disulphides by pyridine nucleotides through an enzyme disulphide and a flavin. seems regulated by SigH. [catalytic activity: NADPH + oxidized thioredoxin = NADP(+) + reduced thioredoxin]; thioredoxin reductase TrxB2 5621552..5622550 Mycobacterium ulcerans Agy99 4553147 YP_908401.1 CDS trxC NC_008611.1 5622547 5622900 D Detected in the cytoplamic, extracellular matrix and secreted fractions by proteomics.; cytoplasmic protein; thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions. forms together with thioredoxin reductase and NADPH a redox active system which donates electrons to a wide variety of different metabolic process (by similarity) seems regulated by SigH.; thioredoxin TrxC 5622547..5622900 Mycobacterium ulcerans Agy99 4553148 YP_908402.1 CDS MUL_5068 NC_008611.1 5623032 5624252 D cytoplasmic protein; function unknown, probably involved in cellular metabolism.; hydrolase 5623032..5624252 Mycobacterium ulcerans Agy99 4553149 YP_908403.1 CDS MUL_5069 NC_008611.1 5624292 5625023 R cytoplasmic protein; hypothetical protein complement(5624292..5625023) Mycobacterium ulcerans Agy99 4553150 YP_908404.1 CDS parB NC_008611.1 5625495 5626478 R Detected in the membrane fraction by proteomics. Also detected in the cytoplasmic fraction by 2D-LC-MS/MS.; secreted protein; involved in chromosome partition. localize to both poles of the predivisional cell following completion of DNA replication. binds to the DNA origin of replication (by similarity); chromosome partitioning protein ParB complement(5625495..5626478) Mycobacterium ulcerans Agy99 4553151 YP_908405.1 CDS parA NC_008611.1 5626475 5627449 R membrane protein; involved in chromosome partition. localize to both poles of the predivisional cell following completion of DNA replication (by similarity); chromosome partitioning protein ParA complement(5626475..5627449) Mycobacterium ulcerans Agy99 4553152 YP_908406.1 CDS gid NC_008611.1 5627780 5628292 R cytoplasmic protein; glucose-inhibited division protein B Gid complement(5627780..5628292) Mycobacterium ulcerans Agy99 4551656 YP_908407.1 CDS MUL_5073 NC_008611.1 5628445 5628993 R domain identity suggests it is a Jag RNA-binding protein; Detected in the cytoplasmic fraction by 2D-LC- MS/MS. Also detected in the membrane fraction by proteomics (2D-LC-MS/MS); membrane protein; hypothetical protein complement(5628445..5628993) Mycobacterium ulcerans Agy99 4551657 YP_908408.1 CDS MUL_5074 NC_008611.1 5629107 5630222 R functions to insert inner membrane proteins into the IM in Escherichia coli; interacts with transmembrane segments; functions in both Sec-dependent and -independent membrane insertion; similar to Oxa1p in mitochondria; putative inner membrane protein translocase component YidC complement(5629107..5630222) Mycobacterium ulcerans Agy99 4551658 YP_908409.1 CDS MUL_5075 NC_008611.1 5630206 5630514 R cytoplasmic protein; hypothetical protein complement(5630206..5630514) Mycobacterium ulcerans Agy99 4551659 YP_908410.1 CDS rnpA NC_008611.1 5630541 5630903 R protein component of RNaseP which catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'terminus; this enzyme also cleaves other RNA substrates; ribonuclease P complement(5630541..5630903) Mycobacterium ulcerans Agy99 4551660 YP_908411.1 CDS rpmH NC_008611.1 5630927 5631070 R in Escherichia coli transcription of this gene is enhanced by polyamines; 50S ribosomal protein L34 complement(5630927..5631070) Mycobacterium ulcerans Agy99 4551661 MUL_0008 tRNA ileT NC_008611.1 10912 10988 D tRNA-Ile 10912..10988 Mycobacterium ulcerans Agy99 4553252 MUL_0009 tRNA alaT NC_008611.1 11149 11224 D tRNA-Ala 11149..11224 Mycobacterium ulcerans Agy99 4553253 MUL_0026 tRNA leuT NC_008611.1 28292 28377 D tRNA-Leu 28292..28377 Mycobacterium ulcerans Agy99 4552753 MUL_0097 tRNA serU NC_008611.1 94684 94773 D tRNA-Ser 94684..94773 Mycobacterium ulcerans Agy99 4549590 MUL_0461 tRNA thrV NC_008611.1 475461 475535 R tRNA-Thr complement(475461..475535) Mycobacterium ulcerans Agy99 4552785 MUL_0530 tRNA pheU NC_008611.1 542140 542216 R tRNA-Phe complement(542140..542216) Mycobacterium ulcerans Agy99 4552245 MUL_0531 tRNA aspT NC_008611.1 542244 542320 R tRNA-Asp complement(542244..542320) Mycobacterium ulcerans Agy99 4552246 MUL_0532 tRNA gluT NC_008611.1 542355 542430 R tRNA-Glu complement(542355..542430) Mycobacterium ulcerans Agy99 4552247 MUL_0533 tRNA lysT NC_008611.1 542554 542629 D tRNA-Lys 542554..542629 Mycobacterium ulcerans Agy99 4552248 MUL_0553 tRNA glyU NC_008611.1 561885 561958 R tRNA-Gly complement(561885..561958) Mycobacterium ulcerans Agy99 4550696 MUL_0672 tRNA tyrT NC_008611.1 698526 698611 D tRNA-Tyr 698526..698611 Mycobacterium ulcerans Agy99 4553395 MUL_0717 tRNA thrT NC_008611.1 754590 754665 D tRNA-Thr 754590..754665 Mycobacterium ulcerans Agy99 4552575 MUL_0718 tRNA metT NC_008611.1 754715 754791 D tRNA-Met 754715..754791 Mycobacterium ulcerans Agy99 4552576 MUL_0723 tRNA trpT NC_008611.1 756607 756682 D tRNA-Trp 756607..756682 Mycobacterium ulcerans Agy99 4552581 MUL_1312 tRNA valV NC_008611.1 1410815 1410889 D tRNA-Val 1410815..1410889 Mycobacterium ulcerans Agy99 4550725 MUL_1461 tRNA asnT NC_008611.1 1579597 1579672 D tRNA-Asn 1579597..1579672 Mycobacterium ulcerans Agy99 4552459 MUL_1607 tRNA leuV NC_008611.1 1743563 1743639 R tRNA-Leu complement(1743563..1743639) Mycobacterium ulcerans Agy99 4552093 MUL_1958 tRNA glnU NC_008611.1 2164284 2164358 D tRNA-Gln 2164284..2164358 Mycobacterium ulcerans Agy99 4551265 MUL_1959 tRNA gluU NC_008611.1 2164428 2164503 D tRNA-Glu 2164428..2164503 Mycobacterium ulcerans Agy99 4551266 MUL_2374 tRNA leuU NC_008611.1 2661544 2661632 D tRNA-Leu 2661544..2661632 Mycobacterium ulcerans Agy99 4550414 MUL_2503 tRNA metU NC_008611.1 2794808 2794884 R tRNA-Phe complement(2794808..2794884) Mycobacterium ulcerans Agy99 4550248 MUL_3231 tRNA proT NC_008611.1 3606913 3606989 R tRNA-Pro complement(3606913..3606989) Mycobacterium ulcerans Agy99 4554208 MUL_3293 tRNA valT NC_008611.1 3668654 3668728 R tRNA-Val complement(3668654..3668728) Mycobacterium ulcerans Agy99 4554288 MUL_3294 tRNA glyT NC_008611.1 3668899 3668974 D tRNA-Gly 3668899..3668974 Mycobacterium ulcerans Agy99 4554289 MUL_3295 tRNA cysU NC_008611.1 3669001 3669074 D tRNA-Cys 3669001..3669074 Mycobacterium ulcerans Agy99 4554290 MUL_3296 tRNA valU NC_008611.1 3669090 3669164 D tRNA-Val 3669090..3669164 Mycobacterium ulcerans Agy99 4554291 MUL_3441 tRNA alaU NC_008611.1 3818273 3818348 R tRNA-Ala complement(3818273..3818348) Mycobacterium ulcerans Agy99 4554491 MUL_3580 tRNA metV NC_008611.1 3971557 3971633 D tRNA-Met 3971557..3971633 Mycobacterium ulcerans Agy99 4553414 MUL_3733 tRNA proU NC_008611.1 4164789 4164865 R tRNA-Pro complement(4164789..4164865) Mycobacterium ulcerans Agy99 4552942 MUL_3734 tRNA glyV NC_008611.1 4165030 4165103 D tRNA-Gly 4165030..4165103 Mycobacterium ulcerans Agy99 4552943 MUL_3767 tRNA argW NC_008611.1 4206143 4206219 D tRNA-Arg 4206143..4206219 Mycobacterium ulcerans Agy99 4549733 MUL_3791 tRNA hisT NC_008611.1 4232461 4232536 D tRNA-His 4232461..4232536 Mycobacterium ulcerans Agy99 4554334 MUL_3806 tRNA lysU NC_008611.1 4246228 4246302 D tRNA-Lys 4246228..4246302 Mycobacterium ulcerans Agy99 4554349 MUL_3903 tRNA leuW NC_008611.1 4362302 4362388 R tRNA-Leu complement(4362302..4362388) Mycobacterium ulcerans Agy99 4553719 MUL_3973 tRNA argV NC_008611.1 4439106 4439181 D tRNA-Arg 4439106..4439181 Mycobacterium ulcerans Agy99 4551397 MUL_4219 tRNA thrU NC_008611.1 4690811 4690886 R tRNA-Thr complement(4690811..4690886) Mycobacterium ulcerans Agy99 4551230 MUL_4260 tRNA proY NC_008611.1 4732665 4732741 D tRNA-Pro 4732665..4732741 Mycobacterium ulcerans Agy99 4551251 MUL_4317 tRNA serV NC_008611.1 4792772 4792860 D tRNA-Ser 4792772..4792860 Mycobacterium ulcerans Agy99 4551320 MUL_4369 tRNA serX NC_008611.1 4850647 4850736 R tRNA-Ser complement(4850647..4850736) Mycobacterium ulcerans Agy99 4550408 MUL_4405 tRNA argU NC_008611.1 4887378 4887453 R tRNA-Arg complement(4887378..4887453) Mycobacterium ulcerans Agy99 4549888 MUL_4406 tRNA serT NC_008611.1 4887483 4887574 R tRNA-Ser complement(4887483..4887574) Mycobacterium ulcerans Agy99 4549889 MUL_4448 tRNA argT NC_008611.1 4934267 4934342 D tRNA-Arg 4934267..4934342 Mycobacterium ulcerans Agy99 4550236 MUL_4618 tRNA leuX NC_008611.1 5119865 5119941 R tRNA-Leu complement(5119865..5119941) Mycobacterium ulcerans Agy99 4552227 MUL_4636 tRNA glnT NC_008611.1 5137336 5137410 D tRNA-Gln 5137336..5137410 Mycobacterium ulcerans Agy99 4550807 MUL_4689 tRNA alaV NC_008611.1 5194291 5194366 R tRNA-Ala complement(5194291..5194366) Mycobacterium ulcerans Agy99 4554376 MUL_3947 rRNA rrf NC_008611.1 4409817 4409932 R 5S ribosomal RNA complement(4409817..4409932) Mycobacterium ulcerans Agy99 4552514 MUL_3948 rRNA rrl NC_008611.1 4410027 4413125 R 23S ribosomal RNA complement(join(4410027..4411532,4411590..4413125)) Mycobacterium ulcerans Agy99 4552515 MUL_3949 rRNA rrs NC_008611.1 4413402 4414940 R 16S ribosomal RNA complement(4413402..4414940) Mycobacterium ulcerans Agy99 4552516