-- dump date   	20140619_161242
-- class       	Genbank::Contig
-- table       	contig_comment
-- id	comment
NC_014751.1	PROVISIONAL REFSEQ: This record has not yet been subject to finalPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is aPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoidesPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The typePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthriticPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this projectPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures andPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University,PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimizePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was preparedPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MUPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome,PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome randomPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminantPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of diseasePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause twoPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovinePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprinePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenicPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, andPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection andPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variablePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indelPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with othersPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions havingPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomicPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomic features implicate multiple evolutionary forces (includingPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomic features implicate multiple evolutionary forces (including horizontal gene transfer and differential gene loss) in shapingPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomic features implicate multiple evolutionary forces (including horizontal gene transfer and differential gene loss) in shaping this group of organisms, and further reveal the basis for some ofPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomic features implicate multiple evolutionary forces (including horizontal gene transfer and differential gene loss) in shaping this group of organisms, and further reveal the basis for some of their distinguishing phenotypic traits. This information may bePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomic features implicate multiple evolutionary forces (including horizontal gene transfer and differential gene loss) in shaping this group of organisms, and further reveal the basis for some of their distinguishing phenotypic traits. This information may be useful in the design of disease control measures. Recently, aPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomic features implicate multiple evolutionary forces (including horizontal gene transfer and differential gene loss) in shaping this group of organisms, and further reveal the basis for some of their distinguishing phenotypic traits. This information may be useful in the design of disease control measures. Recently, a genome of one taxon of this cluster has been syntheticallyPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomic features implicate multiple evolutionary forces (including horizontal gene transfer and differential gene loss) in shaping this group of organisms, and further reveal the basis for some of their distinguishing phenotypic traits. This information may be useful in the design of disease control measures. Recently, a genome of one taxon of this cluster has been synthetically engineered and transplanted to redirect the identity of a distinctPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomic features implicate multiple evolutionary forces (including horizontal gene transfer and differential gene loss) in shaping this group of organisms, and further reveal the basis for some of their distinguishing phenotypic traits. This information may be useful in the design of disease control measures. Recently, a genome of one taxon of this cluster has been synthetically engineered and transplanted to redirect the identity of a distinct recipient taxon of the cluster, thereby ushering in the prospect ofPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomic features implicate multiple evolutionary forces (including horizontal gene transfer and differential gene loss) in shaping this group of organisms, and further reveal the basis for some of their distinguishing phenotypic traits. This information may be useful in the design of disease control measures. Recently, a genome of one taxon of this cluster has been synthetically engineered and transplanted to redirect the identity of a distinct recipient taxon of the cluster, thereby ushering in the prospect of designing synthetic cells with tailored genomic content. In thisPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomic features implicate multiple evolutionary forces (including horizontal gene transfer and differential gene loss) in shaping this group of organisms, and further reveal the basis for some of their distinguishing phenotypic traits. This information may be useful in the design of disease control measures. Recently, a genome of one taxon of this cluster has been synthetically engineered and transplanted to redirect the identity of a distinct recipient taxon of the cluster, thereby ushering in the prospect of designing synthetic cells with tailored genomic content. In this regard the genome sequence of M. leachii type str. PG50 alsoPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomic features implicate multiple evolutionary forces (including horizontal gene transfer and differential gene loss) in shaping this group of organisms, and further reveal the basis for some of their distinguishing phenotypic traits. This information may be useful in the design of disease control measures. Recently, a genome of one taxon of this cluster has been synthetically engineered and transplanted to redirect the identity of a distinct recipient taxon of the cluster, thereby ushering in the prospect of designing synthetic cells with tailored genomic content. In this regard the genome sequence of M. leachii type str. PG50 also informs the possible design of tailored synthetic genomes withPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomic features implicate multiple evolutionary forces (including horizontal gene transfer and differential gene loss) in shaping this group of organisms, and further reveal the basis for some of their distinguishing phenotypic traits. This information may be useful in the design of disease control measures. Recently, a genome of one taxon of this cluster has been synthetically engineered and transplanted to redirect the identity of a distinct recipient taxon of the cluster, thereby ushering in the prospect of designing synthetic cells with tailored genomic content. In this regard the genome sequence of M. leachii type str. PG50 also informs the possible design of tailored synthetic genomes with specific genetic features.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomic features implicate multiple evolutionary forces (including horizontal gene transfer and differential gene loss) in shaping this group of organisms, and further reveal the basis for some of their distinguishing phenotypic traits. This information may be useful in the design of disease control measures. Recently, a genome of one taxon of this cluster has been synthetically engineered and transplanted to redirect the identity of a distinct recipient taxon of the cluster, thereby ushering in the prospect of designing synthetic cells with tailored genomic content. In this regard the genome sequence of M. leachii type str. PG50 also informs the possible design of tailored synthetic genomes with specific genetic features. This project was supported by US Department of Agriculture ARSPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomic features implicate multiple evolutionary forces (including horizontal gene transfer and differential gene loss) in shaping this group of organisms, and further reveal the basis for some of their distinguishing phenotypic traits. This information may be useful in the design of disease control measures. Recently, a genome of one taxon of this cluster has been synthetically engineered and transplanted to redirect the identity of a distinct recipient taxon of the cluster, thereby ushering in the prospect of designing synthetic cells with tailored genomic content. In this regard the genome sequence of M. leachii type str. PG50 also informs the possible design of tailored synthetic genomes with specific genetic features. This project was supported by US Department of Agriculture ARS Project 1940-32000-039-08S at the University of Missouri and thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomic features implicate multiple evolutionary forces (including horizontal gene transfer and differential gene loss) in shaping this group of organisms, and further reveal the basis for some of their distinguishing phenotypic traits. This information may be useful in the design of disease control measures. Recently, a genome of one taxon of this cluster has been synthetically engineered and transplanted to redirect the identity of a distinct recipient taxon of the cluster, thereby ushering in the prospect of designing synthetic cells with tailored genomic content. In this regard the genome sequence of M. leachii type str. PG50 also informs the possible design of tailored synthetic genomes with specific genetic features. This project was supported by US Department of Agriculture ARS Project 1940-32000-039-08S at the University of Missouri and the Office of Science (BER), U.S. Department of Energy, Grant No.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomic features implicate multiple evolutionary forces (including horizontal gene transfer and differential gene loss) in shaping this group of organisms, and further reveal the basis for some of their distinguishing phenotypic traits. This information may be useful in the design of disease control measures. Recently, a genome of one taxon of this cluster has been synthetically engineered and transplanted to redirect the identity of a distinct recipient taxon of the cluster, thereby ushering in the prospect of designing synthetic cells with tailored genomic content. In this regard the genome sequence of M. leachii type str. PG50 also informs the possible design of tailored synthetic genomes with specific genetic features. This project was supported by US Department of Agriculture ARS Project 1940-32000-039-08S at the University of Missouri and the Office of Science (BER), U.S. Department of Energy, Grant No. DE-FC02-02ER63453 to the J. Craig Venter Institute.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002108. Mycoplasma leachii (formerly Mycoplasma sp. 'bovine group 7') is a recently re-named species belonging to the Mycoplasma mycoides phylogenetic cluster of ruminant mycoplasmal pathogens. The type strain PG50 was isolated in Australia in 1963 from an arthritic joint of a calf with mastitis. The culture used for this project was obtained from the Mollicutes Collection of Cultures and Antisera (http://the-iom.org/index.php?id=46), Purdue University, under the designation 'PG50 [N29] bovine group 7'. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate (MU clone 8). The genome, consisting of a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. All taxa comprising the Mycoplasma mycoides cluster infect ruminant animals, in which they display a wide range of disease manifestations and severity. Specific subspecies cause two widespread and economically damaging diseases, contagious bovine pleuropneumonia (CBPP) in cattle and contagious caprine pleuropneumonia (CCPP) in goats. These and other less pathogenic subspecies of the cluster show overlaps in phenotypic traits, and incongruent phylogenetic branching based on different gene sets. Consequently taxa of this group continue to challenge the definition of species, and their selective detection and classification has required multiple approaches. Phase variable expression of surface antigens mediated by reversible indel mutation is also known to occur in two taxa of the group. Comparison of the genome of M. leachii type str. PG50 with others in the Mycoplasma mycoides cluster reveals select regions having distinctive content or topology. Collectively, these genomic features implicate multiple evolutionary forces (including horizontal gene transfer and differential gene loss) in shaping this group of organisms, and further reveal the basis for some of their distinguishing phenotypic traits. This information may be useful in the design of disease control measures. Recently, a genome of one taxon of this cluster has been synthetically engineered and transplanted to redirect the identity of a distinct recipient taxon of the cluster, thereby ushering in the prospect of designing synthetic cells with tailored genomic content. In this regard the genome sequence of M. leachii type str. PG50 also informs the possible design of tailored synthetic genomes with specific genetic features. This project was supported by US Department of Agriculture ARS Project 1940-32000-039-08S at the University of Missouri and the Office of Science (BER), U.S. Department of Energy, Grant No. DE-FC02-02ER63453 to the J. Craig Venter Institute. COMPLETENESS: full length.