-- dump date 20140619_164510 -- class Genbank::CDS -- table cds_note -- id note NP_840102.1 binds to the dnaA-box as an ATP-bound complex at the origin of replication during the initiation of chromosomal replication; can also affect transcription of multiple genes including itself. NP_840104.1 negatively supercoils closed circular double-stranded DNA NP_840112.1 similar to cytochrome P460 precursor - Nitrosomonas europaea gb|AAA62668.1| (U15305) cytochrome P460 precursor [Nitrosomonas europaea], evalue=1.00e-112 NP_840114.1 Catalyzes the conversion of N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate NP_840115.1 TrpG; with TrpE catalyzes the formation of anthranilate and glutamate from chorismate and glutamine; TrpG provides the glutamine amidotransferase activity NP_840120.1 catalyzes the formation of 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and L-glutamine in purine biosynthesis NP_840121.1 COGS group COG0063, predicted sugar kinase, evalue=8e-37 NP_840128.1 10 kDa chaperonin; Cpn10; GroES; forms homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring NP_840129.1 60 kDa chaperone family; promotes refolding of misfolded polypeptides especially under stressful conditions; forms two stacked rings of heptamers to form a barrel-shaped 14mer; ends can be capped by GroES; misfolded proteins enter the barrel where they are refolded when GroES binds; many bacteria have multiple copies of the groEL gene which are active under different environmental conditions; the B.japonicum protein in this cluster is expressed constitutively; in Rhodobacter, Corynebacterium and Rhizobium this protein is essential for growth NP_840131.1 Tig; RopA; peptidyl-prolyl cis/trans isomerase; promotes folding of newly synthesized proteins; binds ribosomal 50S subunit; forms a homodimer NP_840132.1 hydrolyzes proteins to small peptides; with the ATPases ClpA or ClpX, ClpP degrades specific substrates NP_840133.1 binds and unfolds substrates as part of the ClpXP protease NP_840139.1 required for the synthesis of the hydromethylpyrimidine moiety of thiamine NP_840140.1 BacA; phosphatase activity in Escherichia coli not kinase; involved in bacitracin resistance as bacitracin supposedly sequesters UDP disphosphate which reduces the pool of lipid carrier available to the cell NP_840144.1 catalyzes a two-step reaction; charges a cysteine by linking its carboxyl group to the alpha-phosphate of ATP then transfers the aminoacyl-adenylate to its tRNA NP_840146.1 with MotA forms the ion channels that couple flagellar rotation to proton/sodium motive force across the membrane and forms the stator elements of the rotary flagellar machine NP_840147.1 With MotB forms the ion channels that couple flagellar rotation to proton/sodium motive force across the membrane and forms the stator elements of the rotary flagellar machine NP_840152.1 Catalyzes the only substrate-level phosphorylation in the TCA cycle NP_840156.1 Involved in cell division; probably involved in intracellular septation NP_840159.1 catalyzes the formation of 2-octaprenylphenol from 3-octaprenyl-4-hydroxybenzoate NP_840160.1 catalyzes the phosphorylation of the phosphocarrier protein HPr of the bacterial phosphotransferase system NP_840167.1 3 different subfamilies; catalyzes the formation of quinolinate from iminoaspartate and dihydroxyacetone phosphate NP_840173.1 catalyzes the formation of tetrahydrofolate and 2-dehydropantoate from 5,10-methylenetetrahydrofolate and 3-methyl-2-oxobutanoate NP_840174.1 catalyzes the formation of (R)-pantothenate from pantoate and beta-alanine NP_840176.1 3'-5' exoribonuclease specific for small oligoribonuclotides NP_840178.1 4-alpha-hydroxytetrahydrobiopterin dehydratase activity; catalyzes the formation of (6R)-6-(L-erythro-1,2-dihydroxypropyl)-7, 8-dihydro-6H-pterin from (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7, 8-tetrahydro-4a-hydroxypterin; functions in recycling tetrahydrobiopterin (BH4) in phenylalanine hydroxylase reaction NP_840188.1 glycinamide ribonucleotide transformylase; GAR Tfase; catalyzes the synthesis of 5'-phosphoribosylformylglycinamide from 5'-phosphoribosylglycinamide and 10-formyltetrahydrofolate; PurN requires formyl folate for the reaction unlike PurT which uses formate NP_840189.1 catalyzes the formation of 1-(5-phosphoribosyl)-5-aminoimidazole from 2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in purine biosynthesis NP_840195.1 contains glutamine-hydrolyzing domain and glutamine amidotransferase; GMP-binding domain; functions to produce GMP from XMP in the IMP pathway NP_840202.1 Catalyzes the reduction of hydroxypyruvate to form D-glycerate, using NADH as an electron donor NP_840203.1 Timkovich,R.,et al. Biophys. J. 75 (4), 1964-1972 (1998)show the protein precursor begins with the 2nd Met. The mature protein begins at residue 23 (DADL...). NP_840205.1 catalyzes the dehydration of 2,3-dihydroxy-3-methylbutanoate to 3-methyl-2-oxobutanoate in valine and isoleucine biosynthesis NP_840206.1 transfers the N-acyl diglyceride moiety to the prospective N-terminal cysteine in prolipoprotein NP_840207.1 Domain of unknown function DUF140 NP_840209.1 dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE), catalyzes the hydrolysis of N-succinyl-L,Ldiaminopimelate L,L-SDAP to L,L-diaminopimelate and succinate. It is a metalloprotease containing dinuclear active sites. Its structure is similar to the carboxypeptidase G2 from Pseudomonas sp. strain RS-16 and the aminopeptidase from Aeromonas proteolytica. NP_840236.1 An endonuclease that specifically degrades the RNA strand of RNA-DNA hybrids NP_840239.1 Chain A, Red Copper Protein Nitrosocyanin From Nitrosomonas Europaea pdb|1IBY|B Chain B, Red Copper Protein Nitrosocyanin From Nitrosomonas Europaea pdb|1IBY|C Chain C, Red Copper Protein Nitrosocyanin From Nitrosomonas NP_840240.1 catalyzes the formation of nucleoside triphosphate from ATP and nucleoside diphosphate NP_840245.1 catalyzes the conversion of 2C-methyl-D-erythritol 2,4-clclodiphosphate into 4-hydroxy-3-methyl-2-en-1-yl diphosphate NP_840246.2 catalyzes a two-step reaction, first charging a histidine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class II aminoacyl-tRNA synthetase; forms homodimers; some organisms have a paralogous gene, hisZ, that is similar to hisS and produces a protein that performs the first step in histidine biosynthesis along with HisG NP_840249.1 EngA; essential Neisserial GTPase; synchronizes cellular events by interacting with multiple targets with tandem G-domains; overexpression in Escherichia coli suppresses rrmJ mutation; structural analysis of the Thermotoga maritima ortholog shows different nucleotide binding affinities in the two binding domains NP_840267.1 primary rRNA binding protein; helps nucleate assembly of 30S; binds directly to the 16S rRNA and an intersubunit bridge to the 23S rRNA; autoregulates translation through interactions with the mRNA leader sequence NP_840274.1 catalyzes a two-step reaction, first charging a serine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA NP_840275.1 catalyzes a salvage reaction resulting in the formation of AMP which is metabolically less costly than a de novo synthesis NP_840289.1 in Escherichia coli this protein is wrapped around the base of the L1 stalk NP_840290.1 binds as a heterodimer with protein S6 to the central domain of the 16S rRNA; helps stabilize the platform of the 30S subunit NP_840291.1 weak blast hit to gi|16123684|ref|NP_406997.1| (NC_003143) primosomal replication protein n [Yersinia pestis] Score = 47.0 bits (110), Expect = 3e-05 Identities = 30/102 (29%) NP_840292.1 binds cooperatively with S18 to the S15-16S complex, allowing platform assembly to continue with S11 and S21 NP_840294.1 Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit A is part of the membrane proton channel F0 NP_840295.1 Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit C is part of the membrane proton channel F0 NP_840296.1 Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit B is part of the membrane proton channel. NP_840298.1 produces ATP from ADP in the presence of a proton gradient across the membrane; the alpha chain is a catalytic subunit NP_840299.1 Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is a regulatory subunit NP_840300.1 Produces ATP from ADP in the presence of a proton gradient across the membrane. The beta chain is a regulatory subunit NP_840301.1 part of catalytic core of ATP synthase; alpha(3)beta(3)gamma(1)delta(1)epsilon(1); involved in producing ATP from ADP in the presence of the proton motive force across the membrane NP_840305.1 endonuclease; resolves Holliday structures; forms a complex of RuvABC; the junction binding protein RuvA forms a hexameric ring along with the RuvB helicase and catalyzes branch migration; RuvC then interacts with RuvAB to resolve the Holliday junction by nicking DNA strands of like polarity NP_840306.1 plays an essential role in ATP-dependent branch migration of the Holliday junction NP_840307.1 promotes strand exchange during homologous recombination; RuvAB complex promotes branch migration; RuvABC complex scans the DNA during branch migration and resolves Holliday junctions at consensus sequences; forms hexameric rings around opposite DNA arms; requires ATP for branch migration and orientation of RuvAB complex determines direction of migration NP_840312.1 forms dimers; may be involved in cell envelope integrity; interacts with outer membrane proteins and with the C-terminal domain of inner membrane protein TolA NP_840319.1 in most organisms, only the N-terminal domain is present in a single polypeptide; in some archaea this domain is fused to a kinase domain; this gene is essential for growth in Escherichia coli and Bacillus subtilis; the secreted glycoprotease from Pasteurella haemolytica showed specificity for O-sialoglycosylated proteins; the Pyrococcus structure shows DNA-binding properties, iron-binding, ATP-binding, and AP endonuclease activity NP_840320.1 a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA NP_840323.1 sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; this is the primary sigma factor of bacteria NP_840364.1 catalyzes the removal of N-terminal amino acids from peptides and arylamides; generally Co(II) however activity has been shown for some methionine aminopeptidases with Zn, Fe, or Mn NP_840365.1 RNase PH; tRNA nucleotidyltransferase; forms hexamers in Bacillus subtilis; phosphoroltic 3'-5' exoribonuclease; involved in maturation of tRNA precursors and removes terminal nucleotides near CCA acceptor arms of mature tRNAs NP_840366.1 HAM1-like protein; Rec-dependent growth; RgdB; yggV; it is suspected that this protein functions to remove misincorporated bases such as xanthine or hypoxanthine NP_840367.1 catalyzes the oxygen-independent formation of protoporphyrinogen-IX from coproporphyrinogen-III NP_840372.1 tRNA (guanine-N(7)-)-methyltransferase; catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA by transferring the methyl residue from S-adenosyl-L-methionine NP_840373.1 functions in thiamine (vitamin B1) biosynthesis; in Bacillus subtilis this enzyme catalyzes the formation of thiazole from dehydroxyglycine and 1-deoxy-D-xylulose-5-phosphate and ThiS-thiocarboxylate NP_840385.1 catalyzes the synthesis of acetylphosphate or propionylphosphate from acetyl-CoA or propionyl-CoA and inorganic phosphate; when using propionyl-CoA the enzyme is functioning in the anaerobic pathway catabolizing threonine to propionate NP_840388.1 required for the assembly of the flagellar basal body P-ring NP_840391.1 acts as a scaffold for the assembly of hook proteins onto the flagellar basal body rod; Yersinia, Vibrio parahaemolyticus, Bradyrhizobium and other organisms have 2 copies of some flagellar genes; in V. parahaemolyticus one set used for lateral flagella production and the other is used for the polar flagella production NP_840392.1 the hook connects flagellar basal body to the flagellar filament NP_840393.1 FlgF, with FlgB and C, makes up the proximal portion of the flagellar basal body rod NP_840394.1 makes up the distal portion of the flagellar basal body rod NP_840395.1 part of the basal body which consists of four rings L, P, S, and M mounted on a central rod NP_840396.1 part of the basal body which consists of four rings L, P, S, and M mounted on a central rod; Vibrio parahaemolyticus, Yersinia, Bradyrhizobium and other bacteria have two copies of this and other flagellar genes; the V. parahaemolyticus protein is associated with the polar flagella and the Bradyrhizobium protein is associated with the thick flagellum NP_840399.1 with FlgK acts as a hook filament junction protein to join the flagellar filament to the hook; in Vibrio parahaemolyticus the protein in this cluster is associated with the lateral flagella NP_840410.1 catalyzes the reversible transfer of the terminal phosphate of ATP to form a long chain polyphosphate NP_840411.1 class II aldolase; catalyzes the reversible aldol condensation of dihydroxyacetonephosphate and glyceraldehyde 3-phosphate in the Calvin cycle, glycolysis and gluconeogenesis NP_840412.1 catalyzes the formation of phosphoenolpyruvate from pyruvate NP_840413.1 Converts 3-phospho-D-glycerate to 3-phospho-D-glyceroyl phosphate during the glycolysis pathway NP_840415.1 catalyzes the formation of ribose 5-phosphate and xylulose 5-phosphate from sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate; can transfer ketol groups between several groups; in Escherichia coli there are two tkt genes, tktA expressed during exponential growth and the tktB during stationary phase NP_840419.1 negatively supercoils closed circular double-stranded DNA NP_840420.1 catalyzes the formation of 3-phosphonooxypyruvate and glutamate from O-phospho-L-serine and 2-oxoglutarate; required both in major phosphorylated pathway of serine biosynthesis and in the biosynthesis of pyridoxine NP_840423.1 catalyzes the formation of L-histidinol phosphate from imidazole-acetol phosphate and glutamate in histidine biosynthesis NP_840434.1 catalyzes the formation of spermidine from putrescine and S-adenosylmethioninamine NP_840444.1 SPOUT methyltransferase family protein; crystal structure shows homodimer; in Escherichia coli this protein methylates pseudouridine at position 1915 of the 23S ribosomal RNA NP_840448.1 E1 component; part of pyruvate dehydrogenase; forms a complex with DlaT and LpdC NP_840449.1 catalyzes the formation of 5,10-methenyltetrahydrofolate from 5,10-methylenetetrahydrofolate and subsequent formation of 10-formyltetrahydrofolate from 5,10-methenyltetrahydrofolate NP_840451.1 catalyzes a two-step reaction, first charging an arginine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA synthetase NP_840473.1 in Escherichia coli this protein is involved in the biosynthesis of the hypermodified nucleoside 5-methylaminomethyl-2-thiouridine, which is found in the wobble position of some tRNAs and affects ribosomal frameshifting; shows potassium-dependent dimerization and GTP hydrolysis; also involved in regulation of glutamate-dependent acid resistance and activation of gadE NP_840477.1 in Escherichia coli transcription of this gene is enhanced by polyamines NP_840480.1 catalyzes the formation of L-proline from pyrroline-5-carboxylate NP_840484.1 similar to full-length Gnd, these proteins seems to have a truncated C-terminal 6PGD domainin; in Methylobacillus flagellatus this gene is essential for NAD+-dependent oxidation of 6-phosphogluconate NP_840485.1 catalyzes the formation of D-glucono-1,5-lactone 6-phosphate from D-glucose 6-phosphate NP_840486.1 EF-Tu; promotes GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis; when the tRNA anticodon matches the mRNA codon, GTP hydrolysis results; the inactive EF-Tu-GDP leaves the ribosome and release of GDP is promoted by elongation factor Ts; many prokaryotes have two copies of the gene encoding EF-Tu NP_840487.1 NusE; involved in assembly of the 30S subunit; in the ribosome, this protein is involved in the binding of tRNA; in Escherichia coli this protein was also found to be involved in transcription antitermination; NusB/S10 heterodimers bind boxA sequences in the leader RNA of rrn operons which is required for antitermination; binding of NusB/S10 to boxA nucleates assembly of the antitermination complex NP_840488.1 binds directly near the 3' end of the 23S rRNA, where it nucleates assembly of the 50S subunit; essential for peptidyltransferase activity; mutations in this gene confer resistance to tiamulin NP_840489.1 L4 is important during the early stages of 50S assembly; it initially binds near the 5' end of the 23S rRNA NP_840490.1 binds third domain of 23S rRNA and protein L29; part of exit tunnel NP_840491.1 one of the primary rRNA-binding proteins; required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation NP_840492.1 protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA NP_840493.1 binds specifically to 23S rRNA during the early stages of 50S assembly; makes contact with all 6 domains of the 23S rRNA in the assembled 50S subunit and ribosome; mutations in this gene result in erythromycin resistance; located near peptidyl-transferase center NP_840494.1 forms a complex with S10 and S14; binds the lower part of the 30S subunit head and the mRNA in the complete ribosome to position it for translation NP_840495.1 located in the peptidyl transferase center and may be involved in peptidyl transferase activity; similar to archaeal L10e NP_840497.1 primary binding protein; helps mediate assembly; involved in translation fidelity NP_840498.1 binds to the 23S rRNA between the centers for peptidyl transferase and GTPase NP_840499.1 assembly initiator protein; binds to 5' end of 23S rRNA and nucleates assembly of the 50S; surrounds polypeptide exit tunnel NP_840500.1 part of 50S and 5S/L5/L18/L25 subcomplex; contacts 5S rRNA and P site tRNA; forms a bridge to the 30S subunit in the ribosome by binding to S13 NP_840501.1 located in the peptidyl transferase center and involved in assembly of 30S ribosome subunit; similar to what is observed with proteins L31 and L33, some proteins in this family contain CXXC motifs that are involved in zinc binding; if two copies are present in a genome, then the duplicated copy appears to have lost the zinc-binding motif and is instead regulated by zinc; the proteins in this group do not appear to have the zinc-binding motif NP_840502.1 binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit NP_840503.1 ribosomal protein L6 appears to have arisen as a result of an ancient gene duplication as based on structural comparison of the Bacillus stearothermophilus protein; RNA-binding appears to be in the C-terminal domain; mutations in the L6 gene confer resistance to aminoglycoside antibiotics such as gentamicin and these occur in truncations of the C-terminal domain; it has been localized to a region between the base of the L7/L12 stalk and the central protuberance NP_840504.1 binds 5S rRNA along with protein L5 and L25 NP_840505.1 located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body; contacts S4 and S8; with S4 and S12 plays a role in translational accuracy; mutations in this gene result in spectinomycin resistance NP_840506.1 L30 binds domain II of the 23S rRNA and the 5S rRNA; similar to eukaryotic protein L7 NP_840508.1 forms heterotrimeric complex in the membrane; in bacteria the complex consists of SecY which forms the channel pore and SecE and SecG; the SecG subunit is not essential; in bacteria translocation is driven via the SecA ATPase NP_840509.1 stimulates the activities of the other two initiation factors, IF-2 and IF-3 NP_840510.1 located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA; makes contact with the large subunit via RNA-protein interactions and via protein-protein interactions with L5; contacts P-site tRNA NP_840511.1 located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA; forms part of the Shine-Dalgarno cleft in the 70S ribosome; interacts with S7 and S18 and IF-3 NP_840512.1 primary rRNA binding protein; nucleates 30S assembly; involved in translational accuracy with proteins S5 and S12; interacts with protein S5; involved in autogeneously regulating ribosomal proteins by binding to pseudoknot structures in the polycistronic mRNA; interacts with transcription complex and functions similar to protein NusA in antitermination NP_840513.1 catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Dimerization of the alpha subunit is the first step in the sequential assembly of subunits to form the holoenzyme NP_840514.1 is a component of the macrolide binding site in the peptidyl transferase center NP_840517.1 binds to ssrA RNA (tmRNA) and is required for its successful binding to ribosomes; also appears to function in the trans-translation step by promoting accommodation of tmRNA into the ribosomal A site; SmpB protects the tmRNA from RNase R degradation in Caulobacter crescentus; both the tmRNA and SmpB are regulated in cell cycle-dependent manner; functions in release of stalled ribosomes from damaged mRNAs and targeting proteins for degradation NP_840523.1 involved in methylation of ribosomal protein L3 NP_840525.1 malic enzyme; oxaloacetate-decarboxylating; NAD-dependent; catalyzes the formation of pyruvate form malate NP_840528.1 catalyzes the removal of N-terminal amino acids preferably leucine from various peptides NP_840531.1 valine--tRNA ligase; ValRS; converts valine ATP and tRNA(Val) to AMP PPi and valyl-tRNA(Val); class-I aminoacyl-tRNA synthetase type 1 subfamily; has a posttransfer editing process to hydrolyze mischarged Thr-tRNA(Val) which is done by the editing domain NP_840532.1 catalyzes the formation of coproporphyrinogen from uroporphyrinogen III NP_840547.1 FliP, with proteins FliQ and FliR, forms the core of the central channel in the flagella export apparatus NP_840548.1 similar to MOPB PROTEIN pir||S35275 probable flagellar biosynthesis protein mopB - Erwinia carotovora subsp. atroseptica, evalue=1.00e-16, 50% identity NP_840551.1 interacts with cytoplasmic MS ring of the basal body and may act to stabilize the MotAB complexes which surround the MS ring NP_840592.1 Required for efficient pilin antigenic variation NP_840606.1 catalyzes the formation of D-fructose 6-phosphate from fructose-1,6-bisphosphate NP_840615.1 catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate NP_840629.1 similar to (L31491) ORF1; [Plasmid pTOM9], evalue=4.00e-71, 60% identity NP_840636.1 similar to (NC_002122) yacA [Plasmid ColIb-P9], evalue=3.00e-11, 41% identity NP_840652.1 ThyA; catalyzes formation of dTMP and 7,8-dihydrofolate from 5,10-methylenetetrahydrofolate and dUMP; involved in deoxyribonucleotide biosynthesis; there are 2 copies in some Bacilli, one of which appears to be phage-derived NP_840668.1 binds with the catalytic core of RNA polymerase to produce the holoenzyme; this sigma factor is responsible for the expression of heat shock promoters NP_840673.1 catalyzes the formation of oxaloacetate from phosphoenolpyruvate NP_840674.1 transformation of porphobilinogen to hydroxymethylbilane in porphyrin biosynthesis NP_840678.1 catalyzes the formation of 3,6-dideoxy-D-glycero-D-glycero-4-hexulose NP_840682.1 catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A; involved in coenzyme A biosynthesis NP_840691.1 catalyzes the transfer of a methylene carbon from the methylamine-loaded GcvH protein to tetrahydrofolate, causing the release of ammonia and the generation of reduced GcvH protein NP_840692.1 part of multienzyme complex composed of H, L, P, and T proteins which catalyzes oxidation of glycine to yield carbon dioxide, ammonia, 5,10-CH2-H4folate and a reduced pyridine nucleotide; protein H is involved in transfer of methylamine group from the P to T protein; covalently bound to a lipoyl cofactor NP_840693.1 acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine; forms a heterodimer with subunit 2 to form the P protein NP_840694.1 acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine; forms a heterodimer with subunit 1 to form the P protein NP_840697.1 hydrolyzes P(1),P(4)-bis(5'-adenosyl) tetraphosphate to form 2 ADP NP_840698.1 catalyzes the reduction of 2,3-dihydrodipicolinate to 2,3,4,5-tetrahydrodipicolinate in lysine and diaminopimelate biosynthesis NP_840707.1 Catalyzes the formation of dUTP from dCTP in thymidylate biosynthesis NP_840709.1 methionine--tRNA ligase; MetRS; adds methionine to tRNA(Met) with cleavage of ATP to AMP and diphosphate; some MetRS enzymes form dimers depending on a C-terminal domain that is also found in other proteins such as Trbp111 in Aquifex aeolicus and the cold-shock protein CsaA from Bacillus subtilis while others do not; four subfamilies exist based on sequence motifs and zinc content NP_840720.1 TatA; similar to TatE that is found in some proteobacteria; part of system that translocates proteins with a conserved twin arginine motif across the inner membrane; capable of translocating folded substrates typically those with bound cofactors; similar to a protein import system in thylakoid membranes NP_840722.1 catalyzes the formation of 1-(5-phosphoribosyl)-AMP from 1-(5-phosphoribolsyl)-ATP in histidine biosynthesis NP_840723.1 PR-AMP cyclohydrolase; functions in histidine biosynthesis from PRPP; converts 1-(5-phosphoribosyl)-AMP to 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxyamide during the histidine biosynthesis pathway; binds zinc and magnesium; forms homodimers NP_840724.1 catalyzes the conversion of 5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]- 1-(5-phosphoribosyl)imidazole-4-carboxamideand glutamine to imidazole-glycerol phosphate, 5-aminoimidazol-4-carboxamideribonucleotide and glutamate; the HisF subunit acts as a cyclase NP_840725.1 catalyzes the formation of 5-(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino-l- (5-phosphoribosyl)imidazole-4-carboxamide from 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide NP_840726.1 with HisF IGPS catalyzes the conversion of phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide ribonucleotide phosphate and glutamine to imidazole-glycerol phosphate, 5-aminoimidazol-4-carboxamide ribonucleotide, and glutamate in histidine biosynthesis; the HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of imidazole-glycerol phosphate and 5-aminoimidazol-4-carboxamide ribonucleotide NP_840727.1 catalyzes the dehydration of D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate to 3-(imidazol-4-yl)-2-oxopropyl phosphate in histidine biosynthesis NP_840728.1 catalyzes the formation of L-histidinol phosphate from imidazole-acetol phosphate and glutamate in histidine biosynthesis NP_840730.1 catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP); functions in the nonmevalonate isoprenoid biosynthesis pathway NP_840732.1 catalyzes the formation of 3-dehydroshikimate from 3-dehydroquinate in chorismate biosynthesis NP_840735.1 methylates ribosomal protein L11 at multiple amino acid positions; mutations of these genes in Escherichia coli or Thermus thermophilus has no apparent phenotype NP_840740.1 methionine adenosyltransferase; catalyzes the formation of S-adenosylmethionine from methionine and ATP; methionine adenosyltransferase NP_840741.1 catalyzes the formation of L-homocysteine from S-adenosyl-L-homocysteine NP_840750.1 Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates NP_840763.1 similar to cytochrome-c oxidase (EC 1.9.3.1) chain II [imported] - Bacillus stearothermophilus, evalue=5.00e-12, 36% identity NP_840764.1 dehydratase component, catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate NP_840766.1 catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate in leucine biosynthesis; forms a heterodimer of LeuC/D NP_840767.1 catalyzes the oxidation of 3-isopropylmalate to 3-carboxy-4-methyl-2-oxopentanoate in leucine biosynthesis NP_840768.1 catalyzes the formation of 4-aspartyl phosphate from aspartate 4-semialdehyde NP_840770.1 mediates pseudouridylation (positions 38, 39, 40) at the tRNA anticodon region which contributes to the structural stability NP_840771.1 catalyzes the formation of 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate from N-(5-phospho-beta-D-ribosyl)-anthranilate in tryptophan biosynthesis NP_840772.1 catalyzes the formation of L-tryptophan from L-serine and 1-(indol-3-yl)glycerol 3-phosphate NP_840773.1 catalyzes the formation of indole and glyceraldehyde 3-phosphate from indoleglycerol phosphate in tryptophan biosynthesis NP_840778.1 Catalyzes first step of the de novo purine nucleotide biosynthetic pathway NP_840779.1 catalyzes the conversion of O-succinylhomoserine into homocysteine NP_840780.1 threonine deaminase; threonine dehydratase; in Escherichia coli, IlvA is part of the isoleucine biosynthetic pathway NP_840800.1 COGS group COG0313, Predicted methyltransferases, evalue=3e-63 NP_840806.1 catalyzes the formation of N-carbamoyl-L-aspartate from (S)-dihydroorotate in pyrimidine biosynthesis NP_840811.1 catalyzes the selenophosphate-dependent transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA NP_840831.1 with CobST catalyzes the formation of cobyrinic acid a,c-diamide from hydrogenobyrinic acid a,c-diamide in an ATP-dependent manner; involved in porphyrin and chlorophyll metabolism; vitamin B12 metabolism NP_840833.1 in Streptococcus pneumoniae this gene was found to be essential; structure determination of the Streptococcus protein shows that it is similar to a number of other proteins NP_840834.1 modifies transcription through interactions with RNA polymerase affecting elongation, readthrough, termination, and antitermination NP_840835.1 Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits during initiation of protein synthesis. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex NP_840836.1 associates with free 30S ribosomal subunits; essential for efficient processing of 16S rRNA; in Escherichia coli rbfA is induced by cold shock NP_840837.1 catalyzes isomerization of specific uridines in RNA to pseudouridine; responsible for residues in T loops of many tRNAs NP_840838.1 ATP-binding protein; required for proper cytochrome c maturation NP_840841.1 CycJ; periplasmic heme chaperone that binds heme transiently via a histidine residue and delivers it to newly synthesized and exported c-type cytochromes; requires the ATP hydrolysis activity of the CcmA protein in order to transfer the heme to the apocytochrome; part of the cytochrome c maturation system; periplasmic protein anchored to the inner membrane NP_840847.1 catalyzes the oxidation of malate to oxaloacetate NP_840848.1 Catalyzes the oxidation of dihydrolipoamide to lipoamide NP_840852.1 catalyzes the formation of N-acetyl-L-glutamate from L-glutamate and acetyl-CoA in arginine biosynthesis NP_840853.1 in Escherichia coli RsmE methylates the N3 position of the U1498 base in 16S rRNA; cells lacking this function can grow, but are outcompeted by wild-type; SAM-dependent m(3)U1498 methyltransferase NP_840859.1 The UvrABC repair system catalyzes the recognition and processing of DNA lesions. The beta-hairpin of the Uvr-B subunit is inserted between the strands, where it probes for the presence of a lesion NP_840867.1 similar to (NC_003295) PROBABLE RIBOFLAVIN BIOSYNTHESIS BIFUNCTIONAL RIBD, evalue=1.00e-101, 52% identity NP_840882.1 functions in protein export; can interact with acidic membrane phospholipids and the SecYEG protein complex; binds to preproteins; binds to ATP and undergoes a conformational change to promote membrane insertion of SecA/bound preprotein; ATP hydrolysis appears to drive release of the preprotein from SecA and deinsertion of SecA from the membrane; additional proteins SecD/F/YajC aid SecA recycling; exists in an equilibrium between monomers and dimers; may possibly form higher order oligomers; proteins in this cluster correspond SecA1; SecA2 is not essential and seems to play a role in secretion of a subset of proteins NP_840905.1 catalyzes the formation of 3-deoxy-D-arabino-hept-2-ulosonate 7 phosphate from phosphoenolpyruvate and D-erythrose 4-phosphate, phenylalanine sensitive NP_840926.1 hemoprotein; NADPH dependent; with the alpha subunit (a flavoprotein) catalyzes the reduction of sulfite to sulfide NP_840929.1 catalyzes the reduction of 3'-phosphoadenylyl sulfate into sulfite NP_840930.1 with CysN catalyzes the formation of adenylylsulfate from sulfate and ATP NP_840934.1 similar to (NC_003296) PROBABLE TRANSMEMBRANE NAD(P) TRANSHYDROGENASE (ALPHA SUBUNIT PART 2) TRANSMEMBRANE PROTEIN [Ralstonia solanacearum] evalue=6.00e-34, 69% identity NP_840940.1 catalyzes the formation of (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate and L-aspartate in purine biosynthesis; SAICAR synthase NP_840941.1 With PurE catalyzes the conversion of aminoimidazole ribonucleotide to carboxyaminoimidazole ribonucleotide in the de novo purine nucleotide biosynthetic pathway NP_840945.1 short form of enzyme; requires HisZ for function; catalyzes the formation of N'-5'-phosphoribosyl-ATP from phosphoribosyl pyrophosphate; crucial role in histidine biosynthesis; forms heteromultimer of HisG and HisZ NP_840946.1 catalyzes the oxidation of L-histidinol to L-histidinaldehyde and then to L-histidine in histidine biosynthesis; functions as a dimer NP_840949.1 similar to (NC_003295) PROBABLE FACTOR-FOR-INVERSION-STIMULATION TRANSCRIPTION REGULATOR PROTEIN [Ralstonia solanacearum] evalue=3.00e-17, 50% identity NP_840950.1 involved in de novo purine biosynthesis NP_840951.1 catalyzes the formation of N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-D-ribosylamine and glycine in purine biosynthesis NP_840958.1 catalyzes the transfer of a total of four methyl groups from S-adenosyl-l-methionine (S-AdoMet) to two adjacent adenosine bases A1518 and A1519 in 16S rRNA; mutations in ksgA causes resistance to the translation initiation inhibitor kasugamycin NP_840963.1 similar to (NC_001399) ORF C9 [Ralstonia solanacearum], evalue=6.00e-06, 29% identity NP_840971.1 Involved in peptide bond synthesis; alters the affinity of the ribosome for aminoacyl-tRNA NP_840973.1 Catalyzes the reduction of sulfopyruvate to (R)-sulfolactate much more efficiently than the reverse reaction. Also catalyzes the reduction of oxaloacetate, alpha-ketoglutarate, and to a much lower extent, KHTCA, but not pyruvate. Involved in the biosynthes NP_840985.1 catalyzes the methylthiolation of an aspartic acid residue of the S12 protein of the 30S ribosomal subunit NP_840991.1 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates NP_840996.1 catalyze the formation of cyanophycin which may act to store excess nitrogen NP_840997.1 catalyze the formation of cyanophycin which may act to store excess nitrogen NP_841007.1 The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision NP_841016.1 similar to AMMONIA MONOOXYGENASE gb|AAA66195.1| (L08050) ammonia monooxygenase, 43 kDa subunit [Nitrosomonas europaea], evalue=0.00e+00, 95% identity NP_841018.1 similar to (AB079055) ammonia monooxygenase subunit C2 [Nitrosomonas sp. ENI-11], evalue=1e-160, 95% identity NP_841020.1 InterPro IPR001454 Haloacid dehalogenase/epoxide hydrolase family; COG0637 Predicted phosphatase/phosphohexomutase NP_841023.1 catalyzes the conversion of a phosphate monoester to an alcohol and a phosphate NP_841025.1 This protein is one of the two subunits of integration host factor, a specific DNA-binding protein that functions in genetic recombination as well as in transcriptional and translational control NP_841026.1 catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a tetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 2 subfamily NP_841027.1 catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a heterotetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 1 subfamily NP_841028.1 binds directly to 23S ribosomal RNA prior to in vitro assembly of the 50S ribosomal subunit NP_841031.1 catalyzes a two-step reaction, first charging a threonine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; catalyzes the formation of threonyl-tRNA(Thr) from threonine and tRNA(Thr) NP_841033.1 similar to Cytochrome c-554 precursor (C554) (Hydroxylamine oxidoreductase-linked cytochrome) pir||A59036 cytochrome c554, tetraheme, precursor - Nitrosomonas europaea NP_841035.1 identical to (AB030386) hydroxylamine oxidoreductase [Nitrosomonas sp. ENI-11], evalue=0.00e+00, 100% identity NP_841036.1 catalyzes a sulfuration reaction to synthesize 2-thiouridine at the U34 position of tRNAs NP_841041.1 Catalyzes the conversion of ATP and pantetheine 4'-phosphate to diphosphate and 3'-dephospho-coA NP_841055.1 MraZ; UPF0040; crystal structure shows similarity to AbrB NP_841059.1 involved in cell wall formation; peptidoglycan synthesis; cytoplasmic enzyme; catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-aceylmuramoyl-l-alanyl-d-glutamate NP_841061.1 First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan NP_841062.1 UDP-N-acetylmuramoylalanine--D-glutamate ligase; involved in peptidoglycan biosynthesis; cytoplasmic; catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine during cell wall formation NP_841064.1 UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase; involved in cell wall formation; inner membrane-associated; last step of peptidoglycan synthesis NP_841065.1 Catalyzes the formation of UDP-N-acetylmuramoyl-L-alanine from UDP-N-acetylmuramate and L-alanine in peptidoglycan synthesis NP_841066.1 catalyzes the reduction of UDP-N-acetylglucosamine enolpyruvate to form UDP-N-acetylmuramate in peptidoglycan biosynthesis NP_841067.1 D-alanine--D-alanine ligase; DdlA; DdlB; cytoplasmic; catalyzes the formation of D-alanyl-D-alanine from two D-alanines in peptidoglycan synthesis; there are two forms of this enzyme in Escherichia coli NP_841070.1 GTPase; similar structure to tubulin; forms ring-shaped polymers at the site of cell division; other proteins such as FtsA, ZipA, and ZapA, interact with and regulate FtsZ function NP_841074.1 ATP-binding protein; PstABCS is an ATP dependent phosphate uptake system which is responsible for inorganic phosphate uptake during phosphate starvation NP_841075.1 Catalyzes the conversion of citrate to isocitrate NP_841078.1 catalyzes the phosphorylation of N-acetyl-L-glutamate to form N-acetyl-L-glutamate 5-phosphate NP_841083.1 converts protoheme IX and farnesyl diphosphate to heme O NP_841088.1 involved in the insertion of copper into subunit I of cytochrome C oxidase NP_841094.1 catalyzes the carboxylation of acetyl-CoA to malonyl-CoA; forms a tetramer composed of two alpha (AccA) and two beta (AccD) subunits; one of the two catalytic subunits that can form the acetyl CoA carboxylase enzyme together with a carrier protein NP_841108.1 An RNA-DNA helicase that actively releases nascent mRNAs from paused transcription complexes NP_841109.1 RpmE; there appears to be two types of ribosomal proteins L31 in bacterial genomes; some contain a CxxC motif while others do not; Bacillus subtilis has both types; the proteins in this cluster have the CXXC motif; RpmE is found in exponentially growing Bacilli while YtiA was found after exponential growth; expression of ytiA is controlled by a zinc-specific transcriptional repressor; RpmE contains one zinc ion and a CxxC motif is responsible for this binding; forms an RNP particle along with proteins L5, L18, and L25 and 5S rRNA; found crosslinked to L2 and L25 and EF-G; may be near the peptidyltransferase site of the 50S ribosome NP_841113.1 enolase; catalyzes the formation of phosphoenolpyruvate from 2-phospho-D-glycerate in glycolysis NP_841114.1 CTP synthase; CTP synthase; cytidine triphosphate synthetase; catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; in Escherichia coli this enzyme forms a homotetramer NP_841171.1 MexY; AmrB; involved in resistance to antibiotics; inner membrane protein; belongs to RND (resistance, nodulation, cell division) family of transporters; functions along with MexX and OprM; part of an inducible system that appears to respond to interference with the cellular translation machinery NP_841172.1 MexX; AmrA; involved in resistance to antibiotics; periplasmic membrane fusion protein; functions along with MexY and OprM; part of an inducible system that appears to respond to interference with the cellular translation machinery NP_841183.1 similar to (NC_000919) response regulatory protein (atoC) [Treponema pallidum], evalue=2.00e-86, 39% identity NP_841191.1 similar to (U83995) protease [Porphyromonas gingivalis], evalue=2.00e-22, 40% identity NP_841196.1 leucine--tRNA ligase; LeuRS; class-I aminoacyl-tRNA synthetase; charges leucine by linking carboxyl group to alpha-phosphate of ATP and then transfers aminoacyl-adenylate to its tRNA; due to the large number of codons that tRNA(Leu) recognizes, the leucyl-tRNA synthetase does not recognize the anticodon loop of the tRNA, but instead recognition is dependent on a conserved discriminator base A37 and a long arm; an editing domain hydrolyzes misformed products; in Methanothermobacter thermautotrophicus this enzyme associates with prolyl-tRNA synthetase NP_841197.1 Synthesizes oQ from preQ1 in a single S-adenosylmethionine-requiring step NP_841198.1 Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr) NP_841200.1 part of the preprotein secretory system; when complexed with proteins SecF and YajC, SecDFyajC stimulates the proton motive force-driven protein translocation, and appears to be required for the release of mature proteins from the extracytoplasmic side of the membrane NP_841201.1 forms a complex with SecD and YajC; SecDFyajC stimulates the proton motive force-driven protein translocation; seems to modulate the cycling of SecA by stabilizing its membrane-inserted state and appears to be required for the release of mature proteins from the extracytoplasmic side of the membrane; in some organisms, such as Bacillus subtilis, SecD is fused to SecF NP_841204.1 catalyzes the formation of putrescine from agmatine NP_841205.1 lipoprotein signal peptidase; integral membrane protein that removes signal peptides from prolipoproteins during lipoprotein biosynthesis NP_841206.1 IleRS; catalyzes the formation of isoleucyl-tRNA(Ile) from isoleucine and tRNA(Ile); since isoleucine and other amino acids such as valine are similar, there are additional editing function in this enzyme; one is involved in hydrolysis of activated valine-AMP and the other is involved in deacylation of mischarged Val-tRNA(Ile); there are two active sites, one for aminoacylation and one for editing; class-I aminoacyl-tRNA synthetase family type 1 subfamily; some organisms carry two different copies of this enzyme NP_841216.1 catalyzes the bidirectional exonucleolytic cleavage of DNA NP_841218.1 catalyzes the formation of 1-deoxy-D-xylulose 5-phosphate from pyruvate and D-glyceraldehyde 3-phosphate NP_841220.1 similar protein in Methanocaldococcus converts GTP to 7,8-dihydro-D-neopterin 2',3'-cyclic phosphate as the first step in methanopterin biosynthesis NP_841222.1 Involved in pteridine salvage and antifolate resistance NP_841229.1 bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides NP_841241.1 Converts the D-glycero-beta-D-manno-heptose 1,7-bisphosphate intermediate into D-glycero-beta-D-manno-heptose 1-phosphate NP_841243.1 glycine--tRNA ligase alpha chain; GlyRS; class II aminoacyl tRNA synthetase; tetramer of alpha(2)beta(2); catalyzes a two-step reaction; first charging a glycine molecule by linking its carboxyl group to the alpha-phosphate of ATP; second by transfer of the aminoacyl-adenylate to its tRNA NP_841244.1 Transfers the fatty acyl group on membrane lipoproteins NP_841258.1 similar to (AF145800) immunogenic 75 kDa protein PG4 [Porphyromonas gingivalis], evalue=8.00e-07, 24% identity NP_841330.1 catalyzes the formation of N6-(1,2,-dicarboxyethyl)-AMP from L-aspartate, inosine monophosphate and GTP in AMP biosynthesis NP_841331.1 May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine NP_841336.1 Stimulates the elongation of poly(A) tails NP_841339.1 catalyzes the formation of glutamate 5-phosphate from glutamate in proline biosynthesis NP_841340.1 essential GTPase; exhibits high exchange rate for GTP/GDP; associates with 50S ribosomal subunit; involved in regulation of chromosomal replication NP_841341.1 involved in the peptidyltransferase reaction during translation NP_841344.1 catalyzes the second step in the glutathione biosynthesis pathway, where it synthesizes ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione NP_841365.1 hydrolyzes diadenosine polyphosphate NP_841366.1 catalyzes the formation of prolyl-tRNA(Pro) from proline and tRNA(Pro) NP_841369.1 catalyzes the formation of 2-isopropylmalate from acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis NP_841371.1 catalyzes the decarboxylaton of phospatidyl-L-sering to phosphatidylethanolamine NP_841372.1 catalyzes the formation of (R)-2,3-dihydroxy-3-methylbutanoate from (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and isoleucine biosynthesis NP_841373.1 with IlvI catalyzes the formation of 2-acetolactate from pyruvate, the small subunit is required for full activity and valine sensitivity; E.coli produces 3 isoenzymes of acetolactate synthase which differ in specificity to substrates, valine sensitivity and affinity for cofactors; also known as acetolactate synthase 3 small subunit NP_841378.1 catalyzes the ATP-dependent addition of AMP to a subunit of glutamine synthetase; also catalyzes the reverse reaction - deadenylation; adenylation/deadenylation of glutamine synthetase subunits is important for the regulation of this enzyme NP_841381.1 similar to (NC_003047) CAPSULAR POLYSACCHARIDE BIOSYNTHESIS\EXPORT TRANSMEMBRANE PROTEIN [Sinorhizobium meliloti], evalue 1e-25, 26% identity NP_841435.1 similar to (AF285636) type I polyketide synthase WcbR [Burkholderia mallei], evalue=0.00e+00, 41% identity NP_841443.1 catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate NP_841452.1 similar to (AF073793) ammonia monooxygenase 3 subunit C [Nitrosomonas europaea], evalue=0.00e+00, 100% identity NP_841453.1 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate; involved in isoprenoid and isopentenyl-PP biosynthesis; forms homodimers NP_841460.1 unknown function; YciI from Haemophilus influenzae presents crystal structure similarity to a muconolactone isomerase, but does not seem to catalyze any of the predicted reactions based on sequence and structure similarity NP_841461.1 similar to (AB049412) rubredoxin reductase [Acinetobacter sp. M-1], evalue=7.00e-91, 47% identity NP_841462.1 metalloprotease NP_841464.1 Converts (S)-4-amino-5-oxopentanoate to 5-aminolevulinate during the porphyrin biosynthesis pathway NP_841465.1 Condenses 4-methyl-5-(beta-hydroxyethyl)-thiazole monophosphate and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate to form thiamine monophosphate NP_841469.1 essential respiratory protein A; may be involved in the transfer of iron-sulfur clusters; essential for growth using oxygen or alternate electron acceptors NP_841470.1 catalyzes hydrolysis of 1,6-anhydro bond of anyhydro-N-acetylmuramic acid (anhMurNAc) and phosphorylates anhMurNAc to produce N-acetyl-muramate-6-phosphate; involved in murein recycling NP_841472.1 catalyzes the formation of tyrosyl-tRNA(Tyr) from tyrosine and tRNA(Tyr) NP_841474.1 catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate NP_841477.1 catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine to form methionine NP_841478.1 catalyzes the formation of 2-N(omega)-(L-arginino)succinate from L-citrulline and L-aspartate in arginine biosynthesis, AMP-forming NP_841479.1 catalyzes the formation of L-citrulline from carbamoyl phosphate and L-ornithine in arginine biosynthesis and degradation NP_841480.1 catalyzes the formation of N-acetyl-l-glutamate 5-semialdehyde from 2-oxoglutarate and N(2)-acetyl-L-ornithine NP_841484.1 catalyzes the formation of cysteine from 3-O-acetyl-L-serine and hydrogen sulfide NP_841491.1 with SufCD activates cysteine desulfurase SufS NP_841503.1 catalyzes the formation of dUMP from dUTP NP_841504.1 catalyzes the conjugation of cysteine to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, which is then decarboxylated to form 4'-phosphopantotheine NP_841505.1 Involved in DNA double-strand break repair and recombination. Promotes the annealing of complementary single-stranded DNA and by simulation of RAD51 recombinase NP_841506.1 required for 70S ribosome assembly NP_841507.1 in Escherichia coli BM108, a mutation that results in lack of L33 synthesis had no effect on ribosome synthesis or function; there are paralogous genes in several bacterial genomes, and a CXXC motif for zinc binding and an upstream regulation region of the paralog lacking this motif that are regulated by zinc similar to other ribosomal proteins like L31; the proteins in this group lack the CXXC motif NP_841512.1 catalyzes the formation of O-phospho-L-homoserine from L-homoserine NP_841517.1 protoheme ferro-lyase; catalyzes the insertion of a ferrous ion into protoporphyrin IX to form protoheme; involved in protoheme biosynthesis; in some organisms this protein is membrane-associated while in others it is cytosolic NP_841518.1 Negative regulator of class I heat shock genes (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons NP_841519.1 catalyzes the phosphorylation of NAD to NADP NP_841523.1 catalyzes the reduction of N-acetyl-5-glutamyl phosphate to N-acetyl-L-glutamate 5-semialdehyde in arginine biosynthesis and the reduction of N-acetyl-gamma-aminoadipyl-phosphate to N-acetyl-L-aminoadipate-semialdehyde in lysine biosynthesis; involved in both the arginine and lysine biosynthetic pathways; lysine is produced via the AAA pathway, lysine from alpha-aminoadipate NP_841524.1 forms a direct contact with the tRNA during translation NP_841525.1 in Escherichia coli this protein is one of the earliest assembly proteins in the large subunit NP_841529.1 lipoyl/octanoyltransferase; catalyzes the transfer of the lipoyl/octanoyl moiety of lipoyl/octanoyl-ACP onto lipoate-dependent enzymes like pyruvate dehydrogenase and the glycine cleavage system H protein NP_841530.1 catalyzes the radical-mediated insertion of two sulfur atoms into an acyl carrier protein (ACP) bound to an octanoyl group to produce a lipoyl group NP_841531.1 TtcA; YdaO; catalyzes the thiolation of cytosine 32 in specific tRNAs NP_841567.1 Catalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation NP_841573.1 catalyzes the removal of amino acids from the N termini of peptides NP_841588.1 functions in fatty acid oxidation; converts acyl-CoA and FAD to FADH2 and delta2-enoyl-CoA NP_841612.1 protein associated with Co2+ and Mg2+ efflux NP_841642.1 similar to (NC_003296) GENERAL SECRETION PATHWAY GSPG RELATED TRANSMEMBRANE PROTEIN [Ralstonia solanacearum] evalue=4.00e-46, 53% identity NP_841651.1 involved in lysine biosynthesis; DAP epimerase; produces DL-diaminopimelate from LL-diaminopimelate NP_841653.1 catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template; phosphohydrolase activities include hydrolysis of pyrophosphate, 5'-nucleoside tri- and diphosphates, NADP, and 2'-AMP with the production of Pi, metal-dependent phosphodiesterase activity for 2',3'-cAMP, 2',3'-cGMP, and 2',3'-cCMP, and hydrolysis 2',3'-cyclic substrates with the formation of 2'-nucleotides and 3'-nucleotides; these phosphohydrolase activities are probably involved in the repair of the tRNA 3'-CCA terminus degraded by intracellular RNases NP_841655.1 converts 2-oxoglutarate to glutamate; in Escherichia coli this enzyme plays a role in glutamate synthesis when the cell is under energy restriction; uses NADPH; forms a homohexamer NP_841658.1 one of two methionine synthases in Escherichia coli; MetH catalyzes a methyl transfer reaction from methyltetrahydrofolate to homocysteine to create methionine; requires zinc for activity NP_841659.1 Charges one glutamine molecule and pairs it to its corresponding RNA trinucleotide during protein translation NP_841663.1 glycosyltransferase; polymerizes glycan strands in the peptidoglycan NP_841678.1 some L32 proteins have zinc finger motifs consisting of CXXC while others do not NP_841679.2 involved in acylation of glycerol-3-phosphate to form 1-acyl-glycerol-3 phosphate for use in phospholipid biosynthesis; functions with PlsY NP_841680.1 FabH; beta-ketoacyl-acyl carrier protein synthase III; catalyzes the condensation of acetyl-CoA with malonyl-ACP to initiate cycles of fatty acid elongation; differs from 3-oxoacyl-(acyl carrier protein) synthase I and II in that it utilizes CoA thioesters as primers rather than acyl-ACPs NP_841683.1 carries the fatty acid chain in fatty acid biosynthesis NP_841692.1 activates fatty acids by binding to coenzyme A NP_841696.1 catalyzes production of carbamoyl phosphate from bicarbonate and glutamine in pyrimidine and arginine biosynthesis pathways; forms an octamer composed of four CarAB dimers NP_841698.1 Catalyzes the reversible hydrolysis of the amide bond within dihydroorotate. This metabolic intermediate is required for the biosynthesis of pyrimidine nucleotides NP_841699.1 catalyzes the transfer of the carbamoyl moiety from carbamoyl phosphate to L- aspartate in pyrimidine biosynthesis NP_841700.1 regulates pyrimidine biosynthesis by binding to the mRNA of the pyr genes, also has been shown to have uracil phosphoribosyltransferase activity NP_841701.1 similar to RuvC resolvase with substantial differences; NMR structural information suggests this protein is monomeric; unknown cellular function NP_841705.1 binds to lower part of 30S body where it stabilizes two domains; required for efficient assembly of 30S; in Escherichia coli this protein has nuclease activity NP_841706.1 Essential for efficient processing of 16S rRNA NP_841707.1 methylates guanosine-37 in various tRNAs; uses S-adenosyl-L-methionine to transfer methyl group to tRNA NP_841708.1 this protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site NP_841718.1 CMP-2-keto-3-deoxyoctulosonic acid synthetase; catalyzes the formation of CMP-3-deoxy-D-manno-octulosonate from CTP and 3-deoxy-D-manno-octulosonate which is incorporated into LPS NP_841719.1 similar to (NC_003296) PROBABLE 2,4-DIHYDROXYHEPT-2-ENE-1,7-DIOICACID ALDOLASE PROTEIN [Ralstonia solanacearum] evalue=3.00e-40, 37% identity NP_841736.1 may be involved in chromosome condensation; overexpression in Escherichia coli protects against decondensation by camphor; overexpressing the protein results in an increase in supercoiling NP_841737.1 This protein performs the mismatch recognition step during the DNA repair process NP_841739.1 RNH2; RNase HII; binds manganese; endonuclease which specifically degrades the RNA of RNA-DNA hybrids NP_841740.1 in Pseudomonas aeruginosa this enzyme is a trimer of dimers; essential for membrane formation; performs third step of type II fatty acid biosynthesis; catalyzes dehydration of (3R)-hydroxyacyl-ACP to trans-2-acyl-ACP NP_841744.1 catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate NP_841747.1 Rrf; Frr; ribosome-recycling factor; release factor 4; RF4; recycles ribosomes upon translation termination along with release factor RF-3 and elongation factor EF-G; A GTPase-dependent process results in release of 50S from 70S; inhibited by release factor RF-1; essential for viability; structurally similar to tRNAs NP_841748.1 Catalyzes the phosphorylation of UMP to UDP NP_841749.1 EF-Ts; functions during elongation stage of protein translation; forms a dimer; associates with EF-Tu-GDP complex and promotes exchange of GDP to GTP resulting in regeneration of the active form of EF-Tu NP_841750.1 one of the last subunits in the assembly of the 30S subunit; absence of S2 does not inhibit assembly but results in an inactive subunit NP_841759.1 catalyzes a two-step reaction, first charging a tryptophan molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA NP_841764.1 involved in the modulation of the specificity of the ClpAP-mediated ATP-dependent protein degradation; binds to the N-terminal domain of the chaperone ClpA NP_841766.1 involved in fifth step of pyrimidine biosynthesis; converts orotidine 5'-phosphate and diphosphate to orotate and 5-phospho-alpha-D-ribose 1-diphosphate NP_841773.1 Catalyzes D-ribose 5-phosphate --> D-ribulose 5-phosphate in the nonoxidative branch of the pentose phosphate pathway NP_841784.1 Catalyzes the phosphorolytic cleavage of 6-oxopurine nucleosides NP_841785.1 cleaves off formyl group from N-terminal methionine residues of newly synthesized proteins; binds iron(2+) NP_841792.1 molecular chaperone NP_841794.1 Catalyzes the transfer of electrons from NADH to quinone NP_841795.1 Catalyzes the transfer of electrons from NADH to quinone NP_841797.1 Catalyzes the transfer of electrons from NADH to quinone NP_841799.1 Catalyzes the transfer of electrons from NADH to quinone NP_841801.1 Catalyzes the transfer of electrons from NADH to ubiquinone NP_841803.1 Catalyzes the transfer of electrons from NADH to quinone NP_841804.1 Catalyzes the transfer of electrons from NADH to quinone NP_841805.1 Catalyzes the transfer of electrons from NADH to quinone NP_841806.1 The point of entry for the majority of electrons that traverse the respiratory chain eventually resulting in the reduction of oxygen NP_841809.1 Reversibly isomerizes the ketone sugar dihydroxyacetone phosphate to the aldehyde sugar glyceraldehyde-3-phosphate NP_841810.1 2,3-bisphosphoglycerate-dependent; catalyzes the interconversion of 2-phosphoglycerate to 3-phosphoglycerate NP_841834.1 ChvD; in Agrobacterium tumefaciens, mutations in both Walker boxes were found to affect virulence NP_841836.1 Sms; stabilizes the strand-invasion intermediate during the DNA repair; involved in recombination of donor DNA and plays an important role in DNA damage repair after exposure to mutagenic agents NP_841838.1 catalyzes the formation of malyl-CoA from malate and CoA NP_841839.1 Catalyzes the only substrate-level phosphorylation in the TCA cycle NP_841847.1 translation-associated GTPase; the crystal structure of the Haemophilus influenzae YchF protein showed similarity to the yeast structure (PDB: 1NI3); fluorescence spectroscopy revealed nucleic acid binding; the yeast protein YBR025c interacts with the translation elongation factor eEF1 NP_841848.1 Enables the recycling of peptidyl-tRNAs produced at termination of translation NP_841849.1 the Ctc family of proteins consists of two types, one that contains the N-terminal ribosomal protein L25 domain only which in Escherichia coli binds the 5S rRNA while a subset of proteins contain a C-terminal extension that is involved in the stress response NP_841850.1 catalyzes the formation of 5-phospho-alpha-D-ribose 1-phosphate from D-ribose 5-phosphate and ATP NP_841851.1 An essential enzyme in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis NP_841853.1 works in conjunction with MinC and MinD to enable cell division at the midpoint of the long axis of the cell NP_841858.1 involved in potassium uptake; found to be peripherally associated with the inner membrane in Escherichia coli; contains an NAD-binding domain NP_841862.1 catalyzes the conversion of 4-Hydroxybenzoate into 3-octaprenyl-4-hydroxybenzoate, as part of the ubiquinone biosynthesis pathway NP_841870.1 necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites; arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus NP_841872.1 catalyzes branch migration in Holliday junction intermediates NP_841874.1 adds enolpyruvyl to UDP-N-acetylglucosamine as a component of cell wall formation; gram-positive bacteria have 2 copies of MurA which are active NP_841876.1 catalyzes the formation of arginine from (N-L-arginino)succinate NP_841881.1 regulates chemotaxis by demethylation of methyl-accepting chemotaxis proteins NP_841882.1 catalyzes the conversion of glutamine residues to glutamate on methyl-accepting chemotaxis receptors NP_841888.1 similar to (NC_003296) PROBABLE CHEMOTAXIS SENSOR HISTIDINE KINASE TRANSCRIPTION REGULATOR PROTEIN [Ralstonia solanacearum] evalue=0.00e+00, 51% identity NP_841891.1 an Escherichia coli mutant results in accumulation of octaprenylphenol and no ubiquinone; functions in the formation of 2-octaprenyl-6-hydroxy-phenol from 2-octaprenylphenol in ubiquinone (coenzyme Q) biosynthesis; similar to eukaryotic proteins that exhibit kinase functions NP_841898.1 catalyzes the conversion of the propionic acid groups of rings I and III to vinyl groups during heme synthesis NP_841899.1 catalyzes the formation of chorismate from 5-O-(1-carboxyvinyl)-3-phosphoshikimate in aromatic amino acid biosynthesis NP_841914.1 catalyzes the formation of oxaloacetate from L-aspartate NP_841915.1 catalyzes the transamination of the branched-chain amino acids to their respective alpha-keto acids NP_841923.1 subunit A of antiporter complex involved in resistance to high concentrations of Na+, K+, Li+ and/or alkali; in S. meliloti it is known to be involved with K+ NP_841924.1 subunit C of antiporter complex involved in resistance to high concentrations of Na+, K+, Li+ and/or alkali NP_841925.1 subunit D of antiporter complex involved in resistance to high concentrations of Na+, K+, Li+ and/or alkali; contains an oxidoreductase domain; catalyzes the transfer of electrons from NADH to ubiquinone NP_841926.1 subunit E of antiporter complex involved in resistance to high concentrations of Na+, K+, Li+ and/or alkali; in S. meliloti it is known to be involved specifically with K+ transport NP_841927.1 subunit F of antiporter complex involved in resistance to high concentrations of Na+, K+, Li+ and/or alkali; in S. meliloti it is known to be involved specifically with K+ transport NP_841928.1 subunit G of antiporter complex involved in resistance to high concentrations of Na+, K+, Li+ and/or alkali NP_841935.1 recognizes the termination signals UAG and UAA during protein translation a specificity which is dependent on amino acid residues residing in loops of the L-shaped tRNA-like molecule of RF1; this protein is similar to release factor 2 NP_841936.1 catalyzes the formation of glutamate-1-semialdehyde from glutamyl-tRNA(Glu) and NADPH; the second step of the pathway is catalyzed by glutamate-1-semialdehyde aminomutase which results in the formation of 5-aminolevulinic acid; functions in porphyrin (tetrapyrroles) biosynthesis; the crystal structure showed a C-terminal dimerization domain that appears to be absent in Chlamydial proteins NP_841943.1 type III RuBisCO; involved in carbon fixation NP_841952.1 Catalyzes a two-step reaction, first charging an alanyl molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA NP_841954.1 catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs NP_841956.1 catalyzes the formation of D-fructose 1,6-bisphosphate from D-fructose 6-phosphate in glycolysis NP_841957.1 pyrophosphate-energized proton pump; pyrophosphate-energized inorganic pyrophosphatase; H+-PPase; can cleave pyrophosphate to two phosphates; can generate a proton motive force and drive pyrophosphate synthesis when PMF is sufficient NP_841966.1 chaperone Hsp40; co-chaperone with DnaK; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion NP_841967.1 heat shock protein 70; assists in folding of nascent polypeptide chains; refolding of misfolded proteins; utilizes ATPase activity to help fold; co-chaperones are DnaJ and GrpE; multiple copies in some bacteria NP_841968.1 with DnaK and DnaJ acts in response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins; may act as a thermosensor NP_841969.1 Catalyzes two discrete reactions in the de novo synthesis of purines: the cleavage of adenylosuccinate and succinylaminoimidazole carboxamide ribotide NP_841977.1 type 1 subfamily; involved in last step of pyrimidine biosynthesis; converts orotidine 5'-phosphate to UMP and carbon dioxide; OMP decarboxylase; OMPDCase; OMPdecase NP_841979.1 in Escherichia coli this protein is involved in binding to the leader sequence of mRNAs and is itself bound to the 30S subunit; autoregulates expression via a C-terminal domain; in most gram negative organisms this protein is composed of 6 repeats of the S1 domain while in gram positive there are 4 repeats; the S1 nucleic acid-binding domain is found associated with other proteins NP_841980.1 Catalyzes the formation of (d)CDP from ATP and (d)CMP NP_841981.1 catalyzes the formation of 5-O-(1-carboxyvinyl)-3-phosphoshikimate from phosphoenolpyruvate and 3-phosphoshikimate in tryptophan biosynthesis NP_841983.1 decatenates replicating daughter chromosomes NP_841987.1 cleaves off formyl group from N-terminal methionine residues of newly synthesized proteins; binds iron(2+) NP_841992.1 similar to (NC_003112) nitrogen assimilation regulatory protein NtrX [Neisseria meningitidis MC58], evalue=2.00e-95, 46% identity NP_841993.1 IPP transferase; isopentenyltransferase; involved in tRNA modification; in Escherichia coli this enzyme catalyzes the addition of a delta2-isopentenyl group from dimethylallyl diphosphate to the N6-nitrogen of adenosine adjacent to the anticodon of tRNA species that read codons starting with uracil; further tRNA modifications may occur; mutations in miaA result in defects in translation efficiency and fidelity NP_841997.1 catalyzes the formation of shikimate 3-phosphate from shikimate in aromatic amino acid biosynthesis NP_841998.1 catalyzes the formation of 3-dehydroquinate from 3-deoxy-arabino-heptulonate 7-phosphate; functions in aromatic amino acid biosynthesis NP_841999.1 dGTPase family type 2 subfamily; presumably hydrolyzes dGTP to deoxyguanosine and triphosphate NP_842013.1 SohA; PrlF; involved in protein secretion; when overproduced or mutated, it induces growth defect and increased export of a reporter protein; a PrlF mutation induces the activity of the Lon protease, and a Lon-deficient strain suppresses the phenotype conferred by the PrlF mutation NP_842033.1 similar to (AF112478) bifunctional polymerase [Neisseria meningitidis], evalue=8.00e-46, 30% identity NP_842039.1 catalyzes the transfer of a segment of a 1,4-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain NP_842040.1 catalyzes the formation of ADP-glucose and diphosphate from ATP and alpha-D-glucose 1-phosphate NP_842052.1 similar to Cytochrome c-554 precursor (C554) (Hydroxylamine oxidoreductase-linked cytochrome) pir||A59036 cytochrome c554, tetraheme, precursor - Nitrosomonas europaea NP_842054.1 similar to (AB030386) hydroxylamine oxidoreductase [Nitrosomonas sp. ENI-11], evalue=0.00e+00, 95% identity NP_842055.1 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Subunit beta' binds to sigma factor allowing it to bind to the -10 region of the promoter NP_842056.1 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates; beta subunit is part of the catalytic core which binds with a sigma factor to produce the holoenzyme NP_842057.1 present in two forms; L12 is normal, while L7 is aminoacylated at the N-terminal serine; the only multicopy ribosomal protein; 4:1 ratio of L7/L12 per ribosome; two L12 dimers bind L10; critically important for translation efficiency and fidelity; stimulates GTPase activity of translation factors NP_842058.1 binds the two ribosomal protein L7/L12 dimers and anchors them to the large ribosomal subunit NP_842059.2 in Escherichia coli and Methanococcus, this protein autoregulates expression; the binding site in the mRNA mimics the binding site in the 23S rRNA NP_842060.1 binds directly to 23S ribosomal RNA NP_842061.1 Modulates Rho-dependent transcription termination NP_842062.1 EF-Tu; promotes GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis; when the tRNA anticodon matches the mRNA codon, GTP hydrolysis results; the inactive EF-Tu-GDP leaves the ribosome and release of GDP is promoted by elongation factor Ts; many prokaryotes have two copies of the gene encoding EF-Tu NP_842063.1 EF-G; promotes GTP-dependent translocation of the ribosome during translation; many organisms have multiple copies of this gene NP_842064.1 binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit NP_842065.1 interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone; located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side; mutations in the S12 gene confer streptomycin resistance NP_842074.1 similar to (AB079055) ammonia monooxygenase subunit C2 [Nitrosomonas sp. ENI-11], evalue=1.0e-160, 95% identity NP_842079.1 in some organisms this protein is a transmembrane protein while in others it is periplasmic; involved in some organisms with other components of the MreBCD complex and with penicillin binding proteins in the periplasm or cell wall NP_842080.1 functions in MreBCD complex in some organisms NP_842081.1 allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; some Mycoplasma proteins contain an N-terminal fusion to an unknown domain NP_842082.1 allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA NP_842083.1 allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA NP_842090.1 forms a junction between the M-ring and FlgB during flagella biosynthesis NP_842091.1 similar to (NC_002505) sigma-54 dependent response regulator [Vibrio cholerae], evalue=1.00e-116, 52% identity NP_842108.1 Leon G., Roy P.H., J. Bact. 185(6):2036-2041(2003) NP_842136.1 catalyzes the formation of 4-phospho-L-aspartate from L-aspartate and ATP, in Bacillus, lysine sensitive; regulated by response to starvation. NP_842140.1 catalyzes the reversible formation of D-erythrose 4-phosphate and D-fructose 6-phosphate from sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate NP_842147.1 primary rRNA binding protein; nucleates 30S assembly; involved in translational accuracy with proteins S5 and S12; interacts with protein S5; involved in autogeneously regulating ribosomal proteins by binding to pseudoknot structures in the polycistronic mRNA; interacts with transcription complex and functions similar to protein NusA in antitermination NP_842153.1 catalyzes the dephosphorylation of 2-phosphoglycolate to form glycolate and phosphate NP_842154.1 with component II, the glutamine amidotransferase, catalyzes the formation of anthranilate from chorismate and glutamine NP_842162.1 Catalyzes the phosphorylation of L-glutamate during the proline biosynthesis pathway NP_842171.1 catalyzes the formation of dTDP-D-fucosamine from dTDP-4-oxo-6-deoxy-D-glucose in enterobacterial common antigen biosynthesis NP_842185.1 catalyzes the reversible phosphoryl transfer from adenosine triphosphate (ATP) to thymidine monophosphate (dTMP) to form thymidine diphosphate (dTDP) NP_842190.1 Catalyzes the conversion of acetyl-CoA and L-homoserine to CoA and O-acetyl-L-homoserine NP_842193.1 Leon G., Roy P.H., J. Bact. 185(6):2036-2041(2003) NP_842194.1 Leon G., Roy P.H., J. Bact. 185(6):2036-2041(2003) NP_842195.1 Leon G., Roy P.H., J. Bact. 185(6):2036-2041(2003) NP_842210.1 catalyzes the NAD(P)H-dependent reduction of glycerol 3-phosphate to glycerone phosphate NP_842212.1 molecular chaperone that is required for the normal export of envelope proteins out of the cell cytoplasm; in Escherichia coli this proteins forms a homotetramer in the cytoplasm and delivers proteins to be exported to SecA NP_842215.1 bifunctional arginine biosynthesis protein ArgJ; functions at the 1st and 5th steps in arginine biosynthesis; involved in synthesis of acetylglutamate from glutamate and acetyl-CoA and ornithine by transacetylation between acetylornithine and glutmate NP_842221.1 leucyltransferase; phenylalanyltransferse; functions in the N-end rule pathway; transfers Leu, Phe, Met, from aminoacyl-tRNAs to N-terminal of proteins with Arg or Lys NP_842222.2 Conjugates Arg from its aminoacyl-tRNA to the N-termini of proteins containing an N-terminal aspartate or glutamate NP_842223.1 catalyzes the conversion of dihydroorotate to orotate in the pyrimidine biosynthesis pathway; uses a flavin nucleotide as an essential cofactor; class 2 enzymes are monomeric and compared to the class 1 class 2 possess an extended N terminus, which plays a role in the membrane association of the enzyme and provides the binding site for the respiratory quinones that serve as physiological electron acceptors NP_842231.1 similar to (AF324335) long-chain N-acyl amino acid synthase [uncultured proteobacterium SFB/CJ-2001], evalue=6.00e-55, 48% identity NP_842250.1 nucleotide binding property based on structural studies of Haemophilus influenzae crystallized protein in PDB Accession Number 1IN0 and NMR studies of Escherichia coli YajQ; the YajQ protein from Pseudomonas synringae appears to play a role in activation of bateriophage phi6 segment L transcription NP_842254.1 catalyzes a two-step reaction, first charging an aspartate molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; contains discriminating and non-discriminating subtypes NP_842255.1 converts dATP to dAMP and pyrophosphate NP_842256.1 Essential for recycling GMP and indirectly, cGMP NP_842257.1 Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits NP_842262.1 heat shock protein involved in degradation of misfolded proteins NP_842263.1 heat shock protein involved in degradation of misfolded proteins NP_842264.1 catalyzes the formation of beta-ketovaleryl-CoA from acetyl-CoA and propionyl-CoA NP_842265.1 functions in sugar metabolism in glycolysis and the Embden-Meyerhof pathways (EMP) and in gluconeogenesis; catalyzes reversible isomerization of glucose-6-phosphate to fructose-6-phosphate; member of PGI family NP_842285.1 NADPH-dependent; catalyzes the reduction of 7-cyano-7-deazaguanine to 7-aminomethyl-7-deazaguanine in queuosine biosynthesis NP_842286.1 class II family (does not require metal); tetrameric enzyme; fumarase C; reversibly converts (S)-malate to fumarate and water; functions in the TCA cycle NP_842293.1 DTB synthetase; dethiobiotin synthase; involved in production of dethiobiotin from ATP and 7,8-diaminononanoate and carbon dioxide; contains magnesium NP_842311.1 similar to (NC_003295) PROBABLE FIMBRIAL TYPE-4 ASSEMBLY MEMBRANE TRANSMEMBRANE PROTEIN [Ralstonia solanacearum] evalue=4.00e-37, 39% identity NP_842316.1 E3 component of 2-oxoglutarate dehydrogenase complex; catalyzes the oxidation of dihydrolipoamide to lipoamide NP_842317.1 hydrolyzes the terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides NP_842318.1 Catalyzes the formation of holo-ACP, which mediates the essential transfer of acyl fatty acid intermediates during the biosynthesis of fatty acids and lipids NP_842319.1 involved in the de novo synthesis of pyridoxine (Vitamin B6) NP_842320.1 Era; Escherichia coli Ras-like protein; Bex; Bacillus Era-complementing segment; essential protein in Escherichia coli that is involved in many cellular processes; GTPase; binds the cell membrane through apparent C-terminal domain; mutants are arrested during the cell cycle; Streptococcus pneumoniae Era binds to RNA and Escherichia coli Era binds 16S rRNA and 30S ribosome NP_842321.1 cytoplasmic enzyme involved in processing rRNA and some mRNAs; substrates typically have dsRNA regions; forms a homodimer; have N-terminal nuclease and C-terminal RNA-binding domains; requires magnesium as preferred ion for activity NP_842324.1 binds to the ribosome on the universally-conserved alpha-sarcin loop NP_842328.1 Member of the extracytoplasmic function sigma factors which are active under specific conditions; binds with the catalytic core of RNA polymerase to produce the holoenzyme and directs bacterial core RNA polymerase to specific promoter elements to initiate transcription; this sigma factor is involved in heat shock and oxidative stress response NP_842332.1 catalyzes the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine from N6-(dimethylallyl)adenosine (i(6)A) NP_842334.1 similar to Cytochrome c-554 precursor (C554) (Hydroxylamine oxidoreductase-linked cytochrome) pir||A59036 cytochrome c554, tetraheme, precursor - Nitrosomonas europaea NP_842336.1 similar to (AB030386) hydroxylamine oxidoreductase [Nitrosomonas sp. ENI-11], evalue=0.00e+00, 100% identity NP_842337.1 binds directly to the 16S rRNA and is involved in post-translational inhibition of arginine and ornithine decarboxylase NP_842338.1 Acs; catalyzes the conversion of acetate and CoA to acetyl-CoA NP_842352.1 class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA molecules for lysine that exist in this organism and in addition can charge the tRNAPyl with lysine in the presence of LysRS1 NP_842353.1 catalyzes a two-step reaction, first charging a glutamine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA NP_842356.1 catalyzes the formation of phosphoenolpyruvate from pyruvate NP_842358.1 catalyzes a two-step reaction, first charging a glutamine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA NP_842361.1 catalyzes the formation of phosphoenolpyruvate from pyruvate NP_842363.1 broad specificity; family IV; in Corynebacterium glutamicum this protein can use glutamate, 2-aminobutyrate, and aspartate as amino donors and pyruvate as the acceptor NP_842364.1 catalyzes the formation of L-aspartate 4-semialdehyde from L-homoserine NP_842365.2 catalyzes the formation of L-threonine from O-phospho-L-homoserine NP_842366.1 part of four member succinate dehydrogenase enzyme complex that forms a trimeric complex (trimer of tetramers); SdhA/B are the catalytic subcomplex and can exhibit succinate dehydrogenase activity in the absence of SdhC/D which are the membrane components and form cytochrome b556; SdhC binds ubiquinone; oxidizes succinate to fumarate while reducing ubiquinone to ubiquinol; the catalytic subunits are similar to fumarate reductase NP_842369.1 SucA; E1 component of the oxoglutarate dehydrogenase complex which catalyzes the formation of succinyl-CoA from 2-oxoglutarate; SucA catalyzes the reaction of 2-oxoglutarate with dihydrolipoamide succinyltransferase-lipoate to form dihydrolipoamide succinyltransferase-succinyldihydrolipoate and carbon dioxide NP_842387.1 uses the energy from reduction of ubiquinone-1 to ubiquinol to move Na(+) ions from the cytoplasm to the periplasm NP_842388.1 Part of the NQR complex which consists of NqrA, NqrB, NqrC, NqrD, NqrE and NqrF; NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm; NqrE is probably involved in the second step, the conversion of ubisemiquinone to ubiquinol. NP_842389.1 Part of the NQR complex which catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm NP_842390.1 uses the energy from reduction of ubiquinone-1 to ubiquinol to move Na(+) ions from the cytoplasm to the periplasm NP_842391.1 uses the energy from reduction of ubiquinone-1 to ubiquinol to move Na(+) ions from the cytoplasm to the periplasm NP_842392.1 uses the energy from reduction of ubiquinone-1 to ubiquinol to move Na(+) ions from the cytoplasm to the periplasm NP_842398.1 catalyzes the formation of dihydrodipicolinate from L-aspartate 4-semialdehyde and pyruvate in lysine and diaminopimelate biosynthesis NP_842401.1 With FlhD is involved in the activation of class 2 flagellar genes and as well as a number of other genetic systems NP_842402.1 with FlhC is involved in the activation of class 2 flagellar genes and is involved in the regulation of a number of other genetic systems NP_842416.1 Catalyzes the rate-limiting step in dNTP synthesis NP_842417.1 Catalyzes the rate-limiting step in dNTP synthesis NP_842419.1 similar to (D78321) pyrimidine dimer DNA glycosylase [Micrococcus luteus], evalue=2.00e-37, 52% identity NP_842429.1 involved in the biosynthesis of osmoregulated periplasmic glucans; required for the assembly of the polyglucose structure of glucan NP_842430.1 necessary for biosynthesis of osmoregulated periplasmic glucans possibly involved in the transfer to the periplasmic space NP_842448.1 catalyzes the formation of porphobilinogen from 5-aminolevulinate NP_842449.1 binds guanine nucleotides; in Escherichia coli depletion results in defective cell division and filamentation; in Bacillus subtilis this gene is essential NP_842450.1 type III; catalyzes the formation of (R)-4'-phosphopantothenate from (R)-pantothenate in coenzyme A biosynthesis; type III pantothenate kinases are not subject to feedback inhibition from coenzyme A and have a high Km for ATP NP_842453.1 catalyzes the formation of N-succinyl-2-amino-6-ketopimelate from succinyl-CoA and tetrahydrodipicolinate in the lysine biosynthetic pathway NP_842454.1 catalyzes the formation of succinyldiaminopimelate from N-succinyl-2-amino-6-ketopimelate NP_842466.1 glucose-inhibited division protein B; SAM-dependent methyltransferase; methylates the N7 position of guanosine in position 527 of 16S rRNA NP_842467.1 GidA; glucose-inhibited cell division protein A; involved in the 5-carboxymethylaminomethyl modification (mnm(5)s(2)U) of the wobble uridine base in some tRNAs NP_842472.1 stimulates the release of release factors 1 and 2 from the ribosome after hydrolysis of the ester bond in peptidyl-tRNA has occurred; GDP/GTP-binding protein NP_842476.1 membrane protein responsible for substrate specificity switching from rod/hook-type export to filament-type export NP_842477.1 membrane protein involved in the flagellar export apparatus NP_842478.1 positive regulator of class III flagellar genes NP_842488.1 Blast hits say this is a Type 1 restriction modification system endonuclease R NP_842518.1 McrC protein together with McrB forms the McrBC restriction system; recognizes N4- and C5-methylcytosine (and 5-hydroxy-methylcytosines); appears to act against 5-methylcytosine preceded by a purine residue; MrcC modulates the specificty of McrB and has DNA cleavage activity NP_842520.1 similar to (NC_003295) PROBABLE SIGNAL PEPTIDE PROTEIN [Ralstonia solanacearum], evalue=4.00e-30, 48% identity NP_842536.1 Involved in ubiquinone biosynthesis NP_842541.1 Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases NP_842544.1 catalyzes the formation of riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine NP_842545.1 bifunctional enzyme DHBP synthase/GTP cyclohydrolase II-like protein; functions in riboflavin synthesis NP_842546.1 RibE; 6,7-diimethyl-8-ribityllumazine synthase; DMRL synthase; lumazine synthase; beta subunit of riboflavin synthase; condenses 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate to generate 6,6-dimethyl-8-lumazine (DMRL); riboflavin synthase then uses 2 molecules of DMRL to produce riboflavin (vitamin B12); involved in the last steps of riboflavin biosynthesis; forms a 60mer (icosahedral shell) in both Bacillus subtilis and Escherichia coli; in Bacillus subtilis this 60mer is associated with the riboflavin synthase subunit (alpha) while in Escherichia coli it is not NP_842549.1 similar to (NC_003295) PROBABLE PHOSPHATIDYL-GLYCEROPHOSPHATASE HYDROLASE TRANSMEMBRANE PROTEIN [Ralstonia solanacearum] evalue=2.00e-33, 51% identity